Food Science Commons^™

Microencapsulated Ascorbic Acid: Development, Characterization, And Release Profile In Simulated Gastrointestinal Fluids, Michele Cristina Rodrigues Da Cruz, Camila Augusto Perussello, Maria Lúcia Masson

Articles

The aim of this study was to produce ascorbic acid (AA) microcapsules by complex coacervation, characterize them, and evaluate the release profile in simulated gastrointestinal fluids. The microcapsules were made of gelatin and pectin at concentrations of 1.0, 2.0, and 3.0%. The coacervated microcapsules were assessed for morphology (by optical microscopy and scanning electron microscopy), water activity (Aw), hygroscopicity, solubility, encapsulation efficiency (EE), thermal behavior, and AA release profile. The AA microcapsules were characterized by low solubility, low hygroscopicity, high thermal stability, and low diameter (<10 μm), with EE ranging from 23.7 to 94.3%. The release profile was faster in gastric fluid (pH 1.8) than in intestinal fluid (pH 6.8). The sample containing 2.0% of wall material and 50% (w/w) of the simple emulsion (core) had the highest EE and the best in vitro release profile, with release of only 68% of AA in 120 min in the gastric fluid.

Practical applications Ascorbic acid (AA) is widely used in the food industry as an …

Assessing Environmental Flow Targets Using Pre-Settlement Land Cover: A Swat Modeling Application, Sean J. Zeiger, Jason A. Hubbart

Faculty & Staff Scholarship

Determining environmental flow requirements to sustain aquatic ecosystem health remains a challenge. The purpose of this research was to quantify the extent of current flow alterations relative to baseline hydrologic conditions of a simulated historic flow regime prior to anthropogenic flow disturbance (i.e., pre-settlement flows). Results allowed assessment of the efficacy of environmental flow targets based on pre-settlement land cover in a contemporary mixed-land-use catchment (i.e., urban, agricultural, and forested). Pre-settlement flows were simulated using the Soil and Water Assessment Tool (SWAT). Pre-settlement land cover, based on soil physical characteristics, was used to simulate pre-settlement flows with the SWAT model. …

Exploring The Short-Term Effects Of Probiotic And Prebiotic Supplementation On The Microbiota And Physiology Of Male C57bl/6 Mice, Miguel Angel Rivas

Wayne State University Theses

The gut microbiome may play a role in the development of chronic diseases like obesity, diabetes, and heart disease. Diets including prebiotics or probiotics can alter the abundance of gut bacterial groups and have subsequent health effects. In this study we wanted to establish a method for comparing the benefits of commercial supplements that alter the microbiota by monitoring fecal microbial profiles in male C57BL/6 mice (n = 24) exposed to 15 days of dietary supplementation. A probiotic diet (VSL#3) and a prebiotic diet (potato starch) were compared to a standard diet (n = 8 for each group). Microbial profiles …

The Stability Of Tea Polyphenols As A Food Additive And Its Effect On Streptococcus Mutans, Chia-Liang Huang

Theses, Dissertations and Culminating Projects

Dental caries is one of the most concerning chronic diseases, Streptococcus mutans is one the most prominent contributing bacterium. S. mutans metabolizes sugar in processed foods to demineralize the tooth’s surface leading to tooth caries. This study focuses on whether green tea polyphenols epigallocatechin gallate (EGCG), and/or its modifications, palmitoyl-epigallocatechin gallate (P-EGCG) and epigallocatechin-3-gallate-stearate (EGCG-S), can reduce the number of S. mutans in different concentrations of sucrose. A colony forming unit assay was utilized to test viability. Results suggested that the tea polyphenols were efficient in inhibiting S. mutans up to 5 grams of sucrose per 100ml of artificial saliva …

Green Tea Polyphenols As Potential Food Additives, Danxia Shi

Theses, Dissertations and Culminating Projects

As time has progressed, companies have increasingly produced foods for longer term storage. This has led to an increased use of food preservatives in processed foods. People have now come to expect many foods to have a significantly longer shelf life, and this is a matter of government concern for public health due to foodborne contaminations. Foodborne illnesses have become a common problem caused by bacteria (i.e. Escherichia coli). Finding an effective natural preservative source might be better received by the public and alleviate some health concerns over chemical sources. The most important active ingredient found in green tea is …

Differentiation Of Potential False Positives From Human Saliva Using Raman Spectroscopy For Forensic Purposes, Selina Casadei

Legacy Theses & Dissertations (2009 - 2024)

Traces of body fluids can be found at a crime scene. Being able to identify and differentiate the body fluids while preserving DNA is fundamentally important for forensic applications. Saliva is an important body fluid that can be found on bite marks, cigarette butts, and more, being an excellent source for DNA extraction. Current tests for saliva and other body fluids are destructive and body fluid specific, and are prone to false positives. Our laboratory has developed a universal method for identification of all main body fluids; saliva, semen, sweat, peripheral blood, and vaginal fluid, using Raman spectroscopy combined with …

Effects Of Yogurt Fortification With Different Legumes Protein On The Physio-Chemical, Microbiological, And Rheological Properties, Prachi Pahariya

Electronic Theses and Dissertations

Nowadays, yogurt (a nutritious fermented dairy product) is getting popular around all over the world due to beneficial action provided by bacteria present in yogurt culture. Along with that, diet plans, which are high in protein and low in fat, has become appealing for growing healthy conscious population that makes a huge shift toward plantbased protein consumption. Therefore, the main objective of this research was to study the effects of yogurt fortification with extracted protein of different beans (legumes)- pinto and kidney beans on the physio-chemical (Moisture, Fat, Protein, Ash, Total Solids Content (TSC), pH, Titratable Acidity (TA), Water Holding …

The Effects Of Chitosan On Resveratrol-Loaded Zein Nanoparticles, Brian Sohn

Undergraduate Research Symposium Posters

Resveratrol is a naturally occurring polyphenolic compound showing health benefits, including anti-carcinogenic, anti-microbial, anti-HIV/AIDS, neuro-protective, and cardio-protective activities. It has been proposed as a promising dietary supplement or functional ingredient in foods to lower the prevalence of many chronic diseases. However, due to the molecule’s chemical instability, low bioavailability, and poor water solubility, its application was vastly limited. Therefore, extensive research has been conducted regarding microencapsulation of resveratrol. In this study, resveratrol was double encapsulated with zein, a prolamine protein found in maize, and chitosan, a hydrophilic, linear polysaccharide derived from chitin via a deacetylation reaction. Due to their biocompatible, …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Nutraceutical Snack Prepared From Sprouted Rough Rice And Green Gram And Its Physicochemical Properties And In Vitro Glycemic Index, Rachel Browder, Navam Hettiarachchy, Ronny Horax

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Snacks make up a large portion of the U.S. daily meals, but unhealthy snacks are a concern that can lead to being overweight or obese. Healthy alternatives can be germinated cereals and legumes, which undergo chemical compositional changes producing smaller size molecules for easier digestion and generate bioactives that can have health benefits. The objective of this research was to develop a healthy and nutritional snack chip from germinated rough rice and germinated green gram that will be easier for the body to digest, provide much higher protein than conventional chips or crackers with low glycemic index. Rough rice and …

Determining Millennial Food Buying Preferences: Based On Product Marketing With “Buzzwords”, Victoria N. Hamilton

Theses and Dissertations--Community & Leadership Development

This research focuses on the importance on the Millennial Generation and their perceptions of food buzzwords. Since the Millennial Generation is the largest group purchasing and preparing their own foods, the food industry is becoming dependent on their buying preferences. A survey reflected the participants’ demographics and their buying preferences based on a series of food buzzwords when they are purchasing foods. Results show the Millennial Generation prefers “local” buzzwords. As the Millennial Generation continues to purchase foods for themselves and their families, it can be expected their choices will encourage others to do the same based on their family …

Towards A Healthier Chocolate Formula Which Is Rich In Polyphenols And Low In Fat, Lakmani Tharaka Galaniha

Theses and Dissertations--Nutrition and Food Systems

Chocolate is a food that is craved by many in the world and healthy chocolates have become a current topic in the healthy eating trend. The healthiness of a chocolate depends mainly on the polyphenol, fat and sugar contents. Although the literature serves several interventions to reduce the fat content and total calorie content of chocolates, it does not provide many interventions that consider both improving the polyphenol and reducing the fat content simultaneously. Considering this gap in the literature, this research project sought to develop a chocolate that is both low in fat and high in polyphenol content, without …

Letter From The Dean, Deacue Fields

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

The Relationship Between Early Pregnancy Dietary Intakes And Subsequent Birthweight And Neonatal Adiposity, R.A.K. Kennedy, Laura Mullaney, A.C. O'Higgins, A. Doolan, Daniel Mccartney, M.J. Turner

Articles

Background: Maternal nutrition intakes may influence neonatal birthweight and adiposity; however, inconsistencies within the literature exist. The relationships between maternal dietary intakes in early pregnancy and both birthweight and neonatal adiposity requires elucidation. This study examined the relationship between early pregnancy dietary intakes and subsequent birthweight and neonatal adiposity.

Methods: Women were recruited at their convenience after sonographic confirmation of a singleton pregnancy. Women completed a Willet food frequency questionnaire evaluating habitual food and nutrient intakes at their first antenatal visit. Neonatal body composition was measured using air-displacement plethysmography.

Results: Of the 385 mother-neonate dyads, mean maternal age was 30.8 …

Sodium Caprate Enables The Blood Pressure-Lowering Effect Of Ile- Pro-Pro And Leu-Lys-Pro In Spontaneously Hypertensive Rats By Indirectly Overcoming Pept1 Inhibition, John Gleeson, Jesus Maria Frias, Sinead Ryan, David J. Braydon

Articles

The tripeptides, Ile-Pro-Pro (IPP) and Leu-Lys-Pro (LKP), inhibit angiotensin-converting enzyme (ACE) resulting in lowered blood pressure. Our hypothesis was that the medium chain fatty acid permeation enhancer, sodium caprate (C10), may prevent the decrease in permeability of the tripeptides when PepT1 is inhibited by glycyl-sarcosine (Gly-Sar), a situation that may occur in the presence of food hydrolysates. Using Caco-2 monolayers and isolated rat jejunal tissue, the apparent permeability coefficients (Papp) of [3H]-IPP and [3H]- LKP were assessed in the presence of Gly-Sar with and without C10. Gly-Sar decreased the Papp of both tripeptides across monolayers and isolated jejunal tissue, but …

Lignocellulosic Biorefineries In Europe: Current State And Prospects, Amit Jaiswal, Shady Hassan, Gwilym A. Williams

Articles

In 2012, the European Bioeconomy strategy was launched, defining the bioeconomy as "the 33 production of renewable biological resources and the conversion of these resources and waste 34 streams into value added products". This strategy and its action plans increased the turnover 35 of the total bioeconomy in the EU from 2.09 trillion Euro in 2008 to 2.29 trillion in 2015 .

Translation Of Plasma Technology From The Lab To The Food Industry, Patrick J. Cullen, James Lalor, Laurence Scally, Daniela Boehm, Vladimir Milosavljevic, Paula Bourke, Kevin Keener

Articles

The potential of cold plasma as a food processing aid has been demonstrated for a range of processes and products. The potential applications of plasma technology are extensive and include microbial decontamination, pest control, toxin elimination, food and package functionalisation and many others. However, studies reported to date have principally been at laboratory scale. This paper discusses the status and challenges of transferring the technology to the industry. The major challenges discussed for adoption of atmospheric plasma as a food processing tool by industry are (1) demonstration of product/process specific efficacies; (2) development of process compatible technology designs and scale‐up; …

Lactic Acid Fermentation Of Arthrospira PlatensisIs (Spirulina) Biomass For Probiotic-Based Products, Alberto Niccolai, Emer Shannon, Nissreen Abu-Ghannam, Natascia Biondi, Liliana Rodolfi, Mario R. Tredici

Articles

The first objective of this study was to evaluate the use of lyophilised biomass of the cyanobacterium Arthrospira platensis F&M-C256 as the sole substrate for lactic acid fermentation by the probiotic bacterium Lactobacillus plantarum ATCC 8014. After 48 h of fermentation, the bacterial concentration was 10.6 log CFU mL−1 and lactic acid concentration reached 3.7 g L−1. Lyophilised A. platensis F&M-C256 biomass was shown to be a suitable substrate for L. plantarum ATCC 8014 growth. The second objective of the study was to investigate whether lactic acid fermentation could enhance in vitro digestibility and antioxidant activity of A. platensis biomass. …

The Effect Of Non-Thermal Plasma On The Lipid Oxidation And Microbiological Quality Of Sushi, Piotr Kulawik, Carlos Alvarez, Patrick J. Cullen, Ramon Aznar-Roca, Anne Maria Mullen, Brijesh Tiwari

Articles

The study examined the effect of non-thermal plasma (NTP) on the total viable count and lipid oxidation of two common sushi products: nigiri and hosomaki. Sushi samples were treated with NTP using a dielectric barrier discharge system with 70 and 80kV of potential differences for 5min. The samples were stored at 4°C for 11days and analysed for total aerobic count, moisture and protein content, TBA index and fatty acids composition. Although the effect of NTP on the total aerobic counts was not statistically significant, a tendency in log reduction could be observed, with 1–1.5logcfu/g reduction. Moisture and protein content, as …

Ripeness Attributes Of Arkansas-Grown Peaches And Nectarines At Harvest And During Postharvest Storage, Mary C. Siebenmorgen, Renee T. Threlfall, Margaret Worthington

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Since peaches and nectarines are a valued fresh-market crop worldwide, evaluating postharvest potential helps determine feasibility for commercial markets. The ripeness attributes of 10 peach and nectarine genotypes were evaluated at harvest (day 0) and after 7 and 14 d storage at 4° C. The fruit was hand harvested at tree ripeness (ripened on the tree) and commercial ripeness (ripened during storage). The attributes of the tree-ripened fruit and commercially-ripened fruit varied at harvest and included chlorophyll (0.04-0.86 abs), peach weight (132-264 g), soluble solids (7.23-12.57%), pH (3.18-4.66), titratable acidity (0.16-1.21%), and flesh firmness (6.92-35.72 N). In general, tree-ripened fruit …

Characterization Of Short-Grain Rice Cultivars Grown In Japan, California And Arkansas, Michiyo Nishiwaki, Eric Lii, Andy Mauromoustakos, Ya-Jane Wang

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Arkansas and California are the two leading rice-producing states in the U.S. Arkansas predominantly long- and medium-grain rice and California primarily medium- and short-grain rice. Although short-grain rice accounts for less than 2 % of U.S. rice production, the demand for short-grain rice is rising because of increasing popularity of sushi and sake. Because of its premium price and different applications, short-grain rice may open up new opportunities for rice farmers in Arkansas. The objective of this study was to characterize the physical, physicochemical and texture properties of rice cultivars grown in Arkansas versus in Japan and California. Three short-grain …

Formulation And In Vitro Characterisation Of Fungal Chitosan Nanoparticles Coated With Zein For Improved Oral Delivery Of Selenoamino Acids, Giuliana Vozza

Doctoral

Selenium (Se) is an essential micronutrient in both human and animal nutrition that exists in a wide array of different forms, both organic and inorganic. Selenoamino acids (SeAAs), such as selenocystine (SeCys2), selenomethionine (SeMet), and methylselenocysteine (MSC) are organic species with reported health benefits of cancer prevention, increased fertility and improved immunological status. However, supplementation of SeAAs can be challenging, due to their reported narrow therapeutic range of indices, low bioavailability and increased susceptibility to oxidation. To address this, SeAAs were encapsulated into nanoparticles (NPs) to lower their toxicity profiles in comparison to their native form, in addition to offering …

Protein And Lipid Characterization Of Acheta Domesticus, Bombyx Mori, And Locusta Migratoria Dry Flours, Emily N. Brogan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cricket (Acheta domesticus), silkworm pupae (Bombyx mori), and locust (Locusta migratoria) dry flours were obtained to examine the biochemical properties and composition of the flours. This study aimed to characterize the protein and lipid components of three insect species utilizing proximate composition analyses, amino acid composition analysis, protein solubility, SDS-PAGE, fatty acid composition analysis, and thin layer chromatography to determine lipid classes, and lipid extraction efficiency. Kjeldahl determined the cricket, locust, and silkworm flours contained 72.0%, 53.1%, and 71.2% protein, respectively. All proximate composition analyses were significantly (p< 0.05) different between species. Amino acid composition analysis revealed that the flours contained 3.6-3.9% and 0.90-1.5% of lysine and methionine, respectively. Many countries have cereal grains and legumes as a staple in their diet that contain these limiting amino acids. Essential amino acids were 22% of total amino acids. Protein solubility revealed that the three species studied were most soluble in alkaline environments with highest protein solubility occurring at pH 13 at 66% solubility in silkworm. Lowest solubility occurred in more acidic conditions between pH 4-5. SDS-PAGE revealed five major protein fractions with estimated molecular weights of 27 (cuticle proteins), 41 (arginine kinase), which has been identified as an allergen in shrimp, 42 (actin), 71 (hemocyanin), and 220 myosin) kDa. Soxhlet extraction determined cricket, silkworm, and locust flours contained 15.4%, 33.3%, and 11.4% lipid, respectively. The only omega-3 fatty acid found in the flours was α-linolenic acid. Silkworm flour contained the most α-linolenic acid at 33.3% of total fatty acids, followed by locust (13.7%), and cricket (0.6%). TLC resolved lipids for typical lipid classes. The four major lipid classes noted were triacylglycerol, free fatty acid, cholesterol, and phospholipid. Lipid extraction efficiency found that the organic solvents chloroform and methanol had the highest lipid extraction yield in both cricket (69.3%) and locust (93.0%). Methyl-tertiary-butyl ether (MTBE) extracted lipids most efficiently in the silkworm. Proteins and lipids found in insects need to be isolated to further or commercial use.

Influence Of Dietary Ractopamine And Supranutritional Supplementation Of Vitamin E On Proteome Profile Of Postmortem Beef Longissimus Lumborum Muscle, Hyun Mok Kim

Theses and Dissertations--Animal and Food Sciences

The effects of dietary ingredients on the proteome profile of postmortem beef longissimus lumborum (LL) muscle were evaluated. In the first experiment, the influence of dietary ractopamine on the whole-muscle proteome of beef LL was examined. Five proteins were differentially abundant between ractopamine-fed (RAC) and non-ractopamine fed (CON) groups. The differentially abundant proteins were over-abundant in RAC and were related to muscle structure development (F-actin-capping protein subunit beta-2 and PDZ and LIM domain protein-3), chaperone (heat shock protein beta-1), oxygen transportation (myoglobin), and glycolysis (L-lactate dehydrogenase A chain). These findings indicated that ractopamine influences the abundance of proteins associated with …

Rapid And Simultaneous Determination Of Nutritional Constituents Of United States Grown Oats Using Near Infrared Reflectance Spectroscopy (Nirs), Devendra Paudel

Electronic Theses and Dissertations

Oats is a unique cereal owing to its nutritional and health benefits. Near Infrared Reflectance Spectroscopy (NIRS) is an efficient tool for monitoring the quality of cereal foods. NIRS holds potential for non-destructive multicomponent oat analyses with advantage of large sample throughput, speed, and reduced cost. The purpose of this study was to develop predictive calibration models for estimating beta-glucan, protein, and oil content of US oat cultivars using NIRS and validated AACCI reference methods. A rapid, non-destructive (whole oat groat), and secondary NIRS method was developed to estimate beta-glucan, protein, and oil content based on the standard reference analyses …

Shared Mechanisms Among Probiotic Taxa: Implications For General Probiotic Claims, Mary Ellen Sanders, Andrew K. Benson, Sarah Lebeer, Daniel J. Merenstein, Todd R. Klaenhammer

Department of Food Science and Technology: Faculty Publications

Strain-specificity of probiotic effects has been a cornerstone principle of probiotic science for decades. Certainly, some important mechanisms are present in only a few probiotic strains. But scientific advances now reveal commonalities among members of certain taxonomic groups of probiotic microbes. Some clinical benefits likely derive from these shared mechanisms, suggesting that sub-species-specific, species specific or genus-specific probiotic effects exist. Human trials are necessary to confirm specific health benefits. However, a strain that has not been tested in human efficacy trials may meet the minimum definition of the term ‘probiotic’ if it is a member of a well-studied probiotic species …

Modification Of Wheat Gluten For Improvement Of Binding Capacity With Keratin In Hair, Shukun Wang, Danyang Meng, Sisi Wang, Zhong Zhang, Ruijin Yang, Wei Zhao

Department of Food Science and Technology: Faculty Publications

In this study, enzymatic hydrolysis and cationization with epoxypropyldodecyldimethylammonium chloride of wheat protein, an economic protein complex containing great amount of disulfide bonds, were conducted to improve properties such as solubility and disassociation behaviour for recovery of damaged hair when used in shampoo. The optimal conditions for enzymatic hydrolysis were pH 8.2, 55°C with Alcalase for 60min. After the selected hydrolysis, the degree of hydrolysis, nitrogen solubility index, foaming capacity index, foam stability index, emulsifying activity index and emulsion stability index of hydrolysate with 58.71% of shortchain peptides (less than 1000 Da) were 8.81%, 39.07%, 225%, 56.67%, 9.62m² g …

Development Of Omega-3-Rich Camelina Sativa Seed Oil Emulsions, Henok D. Belayneh, Randy L. Wehling, Yue Zhang, Ozan N. Ciftci

Department of Food Science and Technology: Faculty Publications

Camelina sativa seed is an underutilized oil source rich in omega-3 fatty acids; however, camelina oil is not fully explored for food applications. Its high omega-3 content makes it susceptible to oxidation, which may limit food applications. Therefore, the main objective of this study was to investigate the potential of camelina seed oil to form physically and oxidatively stable emulsions as a potential delivery system for omega-3 fatty acids. Effects of homogenization conditions, namely, pressure (15 MPa-30 MPa), number of passes (1,3,5, and 7), and type of homogenizers (high pressure and high shear) on the structural properties and stability of …

Shared Mechanisms Among Probiotic Taxa: Implications For General Probiotic Claims, Mary Ellen Sanders, Andrew K. Benson, Sarah Lebeer, Daniel J. Merenstein, Todd R. Klaenhammer

Department of Food Science and Technology: Faculty Publications

Strain-specificity of probiotic effects has been a cornerstone principle of probiotic science for decades. Certainly, some important mechanisms are present in only a few probiotic strains. But scientific advances now reveal commonalities among members of certain taxonomic groups of probiotic microbes. Some clinical benefits likely derive from these shared mechanisms, suggesting that sub-species-specific, speciesspecific or genus-specific probiotic effects exist. Human trials are necessary to confirm specific health benefits. However, a strain that has not been tested in human efficacy trials may meet the minimum definition of the term ‘probiotic’ if it is a member of a well-studied probiotic species expressing …

Who/Iuis Allergen Nomenclature: Providing A Common Language, Anna Pomes, Janet M. Davies, Gabriele Gadermaier, Christiane Hilger, Thomas Holzhauser, Jonas Lidholm, Andreas L. Lopata, Geoffrey A. Mueller, Andreas Nandy, Christian Radauer, Sanny K. Chan, Uta Jappe, Jorg Kleine-Tebbe, Wayne R. Thomas, Martin D. Chapman, Marianne Van Hage, Ronald Van Ree, Stefan Vieths, Monika Raulf, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

A systematic nomenclature for allergens originated in the early 1980s, when few protein allergens had been described. A group of scientists led by Dr. David G. Marsh developed a nomenclature based on the Linnaean taxonomy, and further established the World Health Organization/International Union of Immunological Societies (WHO/IUIS) Allergen Nomenclature Sub-Committee in 1986. Its stated aim was to standardize the names given to the antigens (allergens) that caused IgE-mediated allergies in humans. The Sub-Committee first published a revised list of allergen names in 1986, which continued to grow with rare publications until 1994. Between 1994 and 2007 the database was a …