Food Science Commons^™

Fast Examination Of Total Colony Numbers In Electronic Cigarette Liquids Based On Adenosine Triphosphate (Atp) Bioluminescence Method, Zhang Tingting, Feng Yingjie, Yang Zongcan, Liu Xiangzhen, Hao Hui, Zhang Dongyu, Zhu Qi, Zhang Zhan

Food and Machinery

In order to fast evaluate the hygiene situation of electronic cigarette liquids, the fast examination of total colony numbers in electronic cigarette liquids has been achieved which was based on the adenosine triphosphate (ATP) bioluminescence method.The method has been optimized, and the examination results of total colony by ATP method was compared with the results by National standard microbial detection method . Results showed: (1) the optimal temperature for ATP examination was 25 ℃, optimal time for bioluminescence reaction was 30 s, the detection limit of ATP was 2.36 × 10^-12 mol/L, RSD was 3.5%～8.2%, and the sample recovery …

Extraction Optimization, Purification And Characterization Of Polysaccharide From The Roots Of Radix Tetrastigma, Guo Nansheng, Jiang Hedong, Zhang Bing, Deng Zeyuan, Cai Hong

Food and Machinery

The polysaccharide from from roots of Radix Tetrastigma were extracted by water bath, and the yield of polysaccharide was determined by response surface methodology. Based on the single factor test, The optimum conditions were as followed: temperature 96 ℃, ratio of water to liquid 34∶1 （mL/g）, extraction time 2 h. Under the conditions, the yield of polysaccharide was (21.23±0.77)%. After deprotein with sevage method, fractionated and purified by DEAE-Cellulose 52 anion exchange column and Sepharose CL-4B gel fltration column, successively . The purifed polysaccharide was designated as RTP-3-1 and its components, molecular weight, primary structure and domains were identified by …

Optimization Of Polysaccharide Extraction Process From Morinda Officinalis How And Its Biological Activity In Vitro And In Vivo, Liang Xiaojun, Wei Bingdun, Chen Qian, Ma Jinkui, Li Ke, Huang Xiaochen

Food and Machinery

Morinda officinalis polysaccharide is an important bioactive substance, in terms of its anti-depression, immune enhancement, anti-viral and anti-tumor effects, and polysaccharide holds great potential in function food and drug development. In this study, the Box-Behnken design principle and one-factor-at-a-time experiments were employed to optimize the extraction parameters of polysaccharide from Morinda officinalis How in Deqing area, Guangdong province. The in vitro antioxidant activities of extracted polysaccharides were also investigated. The results showed that the optimal extraction conditions were as follows: extraction time was 2 h, extraction times was 3, and solid-liquid ratio was 1∶11 （g/mL）. Under the control of these …

Optimization Of Fermentation Process For Exopolysaccharides Production Of Weissella Cibaria Sj-02, Chen Yuan, Jia Sisi, Hu Lu, Wang Boyang, Liu Shuliang

Food and Machinery

Response surface methodology was used to optimize the components of fermentation medium and the fermentation conditions affecting the exopolysaccharides (EPS) production of Weisssella cinerea SJ-02. Taking EPS production as an index, the effects of carbon sources and nitrogen sources in the medium on the yield of exopolysaccharides were explored. The results showed that using MRS as basal medium, keeping its 2% glucose and replacing peptone with 1.5% soy peptone increased the EPS production of strain SJ-02. The Box-Behnken central combination design was used to study the effects of exopolysaccharides production. The optimal fermentation conditions were as follows: inoculation amount 3.00%, …

Whole Cell Cross-Linking To Discover Host-Microbe Protein Cognate Receptor/Ligand Pairs, Bart C. Weimer, Poyin Chen, Prerak T. Desai, Dong Chen, Jigna Shah

Biological Engineering Faculty Publications

Bacterial surface ligands mediate interactions with the host cell during association that determines the specific outcome for the host–microbe association. The association begins with receptors on the host cell binding ligands on the microbial cell to form a partnership that initiates responses in both cells. Methods to determine the specific cognate partnerships are lacking. Determining these molecular interactions between the host and microbial surfaces are difficult, yet crucial in defining biologically important events that are triggered during association of the microbiome, and critical in defining the initiating signal from the host membrane that results in pathology or commensal association. In …

Almond Milk Powder Fortification With Flaxseed Oil (Linum Usitatissimum) As An Alternative Non-Dairy Milk Powder For Infants And Toddlers, Federico Bueno

LSU Master's Theses

Lactose intolerances in toddlers have become a major concern. Almond milk (AM) has nutritional benefits and a texture like dairy milk without the content of lactose. Because of longer stability and storage, milk is often stored in powdered form. An almond milk fortified with flaxseed oil in powdered form may be an alternative. Flaxseed oil (FO) contains high amounts of alpha-linolenic acid (ALA) which is a precursor to DHA and EPA. The objectives of this study were: the development of an AM spray-dried powder with microencapsulated FO (AMFOP), the determination of the mass and energy balance of that AMFOP production, …

A Retrospective Assessment Of Us Pork Productions: 1960 To 2015, Ben Putman, Jacob Hickman, Prathamesh Bandekar, Marty Matlock, Greg Thoma

Food Systems

The primary goal of this study is to assess the carbon, energy, water and land footprints per kg (2.2 pounds) of live weight (LW) pork produced at five-year increments between 1960 and 2015. This assessment utilizes the Life Cycle Assessment (LCA) methodology, which is a technique to assess the potential environmental impacts associated with a product system by compiling an inventory of relevant energy and material flows, evaluating the associated burdens, and interpreting the results to assist in making more informed decisions and to provide an understanding of the drivers of change over the past 55 years. This LCA is …

Influences Of Spiciness And Visual Color Cue On Salty Taste Intensity Perception In Reduced-Sodium Cheese Dips, Valentina Rosasco Silva

LSU Master's Theses

The current high levels of dietary sodium intake are negatively affecting consumers’ health. Reducing sodium consumption to the recommended amount could help prevent around 2.5 million deaths yearly and save up to $18 billion dollars in health care costs worldwide. The main objective of this research was to evaluate the possibility of increasing the saltiness perception and acceptability of reduced-sodium cheese dips by using the multisensory approach and analyzing gender differences towards salty taste expectations. Two experiments were conducted: I) The combined effects of gender and visual color cue on salty taste expectations, liking, and purchase intent (PI) of cheese …

Synthesis And Characterization Of Polyaniline Agglomerates And Polyaniline Nanoparticles To Use As Printed Sensors To Detect Spoilage Of Catfish During Refrigerated Storage, Vashti Martae Campbell

LSU Master's Theses

There is growing interest in the development of sensors contained in packaging for seafood that are applicable at the consumer level to monitor freshness in a non-destructive manner. Total volatile basic nitrogen (TVB-N) analysis indicates the freshness of seafood. TVB-N analysis is sensitive to the spoilage compounds comprised of ammonia, trimethylamine, dimethylamine, and biogenic amines commonly found in seafood and whose concentration increases when spoilage microbial growth occurs. However, TVB-N analysis is laborious and is not suitable for use at the consumer level. Polyaniline (PANI) is a polymer that may be used as a sensor for detecting TVB-N in seafood. …

The Effect Of Destoning And Enzymatic Pretreatments On The Biofuel Production From Olive Cake, Patrick Tai

Master's Theses

More than 16,000 tons of olive cake was produced in the United States in 2017. Olive cake is a by-product of olive oil extraction, which has limited animal feed potential, and poses an environmental threat when landfilled due to its high organic load and polyphenol content. This residue has potential for biofuel (bioethanol and biomethane) production because it is rich in polysaccharides such as pectin, hemicellulose, and cellulose. Yet, olive cake contains olive stones that can impede its conversion to biofuel. Therefore, two methods of destoning, centrifugation and screening by horizontal screw press, were first compared. Both methods removed an …

Dietary Patterns And Risk Of Colorectal Cancer Subtypes Classified By Fusobacterium Nucleatum In Tumor Tissue, Raaj S. Mehta, Reiko Nishihara, Yin Cao, Mingyang Song, Kosuke Mima, Zhi Rong Qian, Jonathan A. Nowak, Keisuke Kosumi, Tsuyoshi Hamada, Yohei Masugi, Susan Bullman, David A. Drew, Aleksandar D. Kostic, Teresa T. Fung, Wendy S. Garrett, Curtis Huttenhower, Kana Wu, Jeffrey A. Meyerhardt, Xuehong Zhang, Walter C. Willett, Edward L. Giovannucci, Charles S. Fuchs, Andrew T. Chan, Shuji Ogino

Department of Food Science and Technology: Faculty Publications

Importance—Fusobacterium nucleatum appears to play a role in colorectal carcinogenesis through suppression of host immune response to tumor. Evidence also suggests that diet influences intestinal F. nucleatum. However, the role of F. nucleatum in mediating the relationship between diet and the risk of colorectal cancer is unknown.

Objective—To test the hypothesis that the associations of prudent diets (rich in whole grains and dietary fiber) and Western diets (rich in red and processed meat, refined grains, and desserts) with colorectal cancer risk may differ according to the presence of F. nucleatum in tumor tissue.

Design—Prospective cohort study.

Setting—The Nurses’ Health Study …

Structural Characteristics Of Millet Glutenin And Gliadin, Ji Zhongwei, Dai Tiantian, Mao Jian

Food and Machinery

Millet glutenin and gliadin was obtained by alkali extraction and acid precipitation and millet gliadin was distracted by ethanol. Then their structural characteristics were analyzed. SDS-PAGE electrophoresis results showed that the molecular weight of the millet gliadin was small, while millet glutenin was distributed from high molecular weight to low molecular weight. Furthermore, the XRD analysis showed that both the two kinds of proteins were amorphous structures. The secondary structure was measured by circular dichroism. Millet gliadin belonged to all alpha structure, and its order was better than that of millet alkali soluble protein. Moreover, by atomic force microscopy analysis, …

Effect Of Intermolecular Interaction On Gel Formation Of Preserved Egg Yolk, Ai Minmin, Tan Gting, Jiang Aimin, Yang Yijia, You Yuting

Food and Machinery

The preserved eggs were processed by immersion method. The effect of internal interactions on the formation of egg yolk gel during salting and ageing were determined by fluorescence spectrophotometer and FTIR et al. The sum of α-helical and β-turn of the secondary structure of the exterior egg yolk protein decreased by 54.71% under strong alkaline environment, with the surface hydrophobicity decreased significantly (P＜0.05), and the dramatic increase of ion bonds and disulfide bonds promoted the egg yolk proteins to form a gel. The sum of α-helical and β-turn of the secondary structure of the interior egg yolk protein increased by …

Measurement Of Grain Physical Properties Based On Multi-Dimensional Unsteady Thermal Conduction Product Method, Chen Qinghua, Su Guoyong, Sun Meihua, Jiang Kuosheng, Liu Ping

Food and Machinery

Formed constant temperature boundary with constant temperature bath and combined with the brass short cylindrical sample box, the formation of multi-dimensional heat flow is guaranteed in the test sample. The unsteady thermal conductivity multiplication method and parameter estimation method were used to calculate the thermal conductivity and specific heat capacity of grain. At the same time, considering the effect of opening holes on the thermostat, and increasing the difficulty of experimental operation, the ZigBee wireless data acquisition module is used for signal acquisition and transmission. By analyzing the thermophysical parameter sensitivity analysis of corn and paddy, the sensitivity coefficient of …

Analysis Of Chemical Ingredients Changes And Aroma Compounds In Brewing Process Of Mulberry Wine, Yang Fang, Liu Tie, Liu Yan, Wang Sha, Wang Danhong

Food and Machinery

To analyse the variation of reducing sugar, total acid, total flavonoids, ethanol content and aroma compounds in brewing process of mulberry wine. The fermentation processes were: fermentation temperature of 15.3 ℃, inoculation quantity of 8.00 g/L, sucrose content of 12.80 g/100 g, total fermentation time 24 days, and sampling every 3 days to determine reducing sugar, total acid, total flavonoids, and ethanol content of mulberry wine. The aroma components were determined by gas chromatography-mass spectrometry. Results: The reducing sugar in mulberry wine decreased quickly at the first nine days and levelled off. The total acid content were steady between 5~6 …

Effect Of Cooking Methods On The Quality And Nutritive Contents Of Pork, Zhang Jie, He Hang, Xiong Zibiao

Food and Machinery

The effects were andyzed, including different various methods (steaming, boiling, frying and baking) on meat quality, routine nutrient composition, amino acid and fatty acid content of pork. The results showed that the pH value and water content after cooking were significantly lower than those of fresh control (P＜0.05), while, the hardness, elasticity, adhesion, ash, crude protein and PUFA content were significantly increased (P＜0.05). The cooking loss rate of frying and microwave was significantly higher than that of steaming, boiling and baking (P＜0.05). The ash content of boiling have no significant change (P＞0.05), while the other cooking methods were significantly increased …

Aptamer-Based Colorimetric Detection Of Cd2+, Zhao Xu, Wu Shijia, Yue Lin, Wang Zhouping

Food and Machinery

In this study, we presented a simple, rapid and sensitive colorimetric assay for the detection of cadmium (Cd) based on enzyme-linked oligonucleotides. The specific binding of aptamer to Cd²⁺ led to the decrease of horseradish peroxidase(HRP) on the microtiter plate, since HRP could not be bind onto through the hybridization of the aptamer and complementary DNA strand. It resulted in the change of characteristic peaks at 450 nm, helping to quantitatively detect Cd²⁺. Under the optimal conditions, the detection limit could be as low as 0.05 ng/mL, and the linear response for Cd²⁺ in a concentration …

Isolation,Identification,Multilocus Sequence Typing Analysis And Antimicrobial Resistance Of Campylobacter Jejuni Isolated From Commercial Chicken In Northeast China, Fei Peng, Zhao Shengjuan, Jiang Yichao, Jiang Yan, Liu Lili, Xiao Feng, Zhang Huiyun, Tang Haoguo, Kang Huaibin

Food and Machinery

In order to reveal the contamination, population characteristics and antimicrobial resistance of Campylobacter jejuni isolated from commercial chicken in Northeast China, 1 000 chicken samples were collected from the Northeast China. C. jejuni was isolated and identified.The genetic diversity of C. jejuni was analyzed by multilocus sequence typing (MLST). The antimicrobial resistance of C. jejuni to 8 antibiotics was detected by K-B AGAR diffusion method. The results indicated that 62 C. jejuni strains were isolated from 1 000 chicken samples with a contamination rate of 6.2%, and were divided into 14 sequence types (STs). Among them, ST1 and ST5 were …

Mining Of Serotype Specific Genes And Establishment Of A Pcr For Detection Salmonella Enterica Serovar Heidelberg, Zhai Ligong, Guo Yuanxin, Wang Junying, Wang Beibei

Food and Machinery

Salmonella enterica serovar Heidelberg,（SH）is pathogenic serotypes within Salmonella. Using BLAST and PCR validation for all CDSs of SH, four serovar-specific genes were identified including SeHA_C2639, SeHA_C2640, SeHA_C3259 and SeHA_C3258. A PCR protocol was developed by pHAm8 (350 bp) of SH-specific primer and 139-141 (284 bp) of Salmonella-specific primer and evaluated for the detection of SH. The result showed that the 55 strains of serotype of Salmonella and other common foodborne pathogens proved that the PCR assay had very good specificity for the detection of SH. The detection limit was 6.1 × 10² CFU/mL in pure culture. It was shown …

Simultaneous Determination Of Three Prohibited Substances In Fragrance Compound Through Gas Chromatography-Mass Spectrometry, Lu Zhi

Food and Machinery

In this study, a gas chromatography-mass spectrometry (GC/MS) method was established for the determination of geranyl nitrile, 2-pentyl-2-cyclopenten-1-one and phenyl cyanide in fragrance compound, and the method was also validated. The method had good linearity (>0.990 0) with the concentration from 0.000 5 mg/mL to 0.100 0 mg/mL and low limits of detection (LOD) (<0.005 μg/mL) for these three target compounds. And the recovery was from 90% to 116% with relative standard deviation (RSD) from 4.2% to 5.4% at quality control concentration. In a word, the method met the need of trace detection, which can be used to test geranyl nitrile, 2-pentyl-2-cyclopenten-1-one and phenyl cyanide in fragrance compound. When the GC-MS method was applied to detect these three components in different pepppermint fragrance samples, none of them was detected out.

Study On Microwave Drying Based On Temperature And Power Control, Luo Guiyi, Song Chunfang, Li Zhenfeng, Xu Wanxiu

Food and Machinery

Based on a microwave drying system，automatically and continuously regulating microwave power and temperature control, a drying model composed of three different temperature and power controls was used to investigate the potato chips. After analyzing the drying quality and energy consumption of the three drying models, a good microwave drying process for potato chips was found. The research showed that the microwave drying without temperature control was easy to overheat in the last drying stage and made the product burnt. However, the optimal temperature control effect and product quality could be obtained by combining the variable microwave power with the feedback …

Study On The Wet Preparation Of Lotus Leaf Powder And Its Grinding Kinetics In The Stirred Media Mill, Yu Jianfeng, Zhao Jiang, Lou Qi, Liang Jie, Wang Li

Food and Machinery

The stirring medium mill was employed to prepare lotus leaf powder. The Variation of particle size of lotus leaf powder within 30 min at different stirring speeds were investigated. The grinding kinetics of the lotus leaf powder was studied using the analytical model of the crushing process. The grinding chamber shear rate distribution and the average collision energy of the grinding media were analyzed at different stirring speeds coupling CFD-DEM method. The results showed that the grinding rate of the lotus leaf powder increased with the increase of the stirring speeds in the range of 500～3 000 r/min. The fluid …

The Research And Application Of Polynomial Fitting In Cam Curve Design Of Cap Screwing Machine, Wu Jia, He Xueming, He Kai, Zhang Rong

Food and Machinery

In view of the excessive local control conditions, the power of the cam curve designed by traditional polynomial interpolation method got too high to control, which was not conducive to the processing of capping machine cam. The global 5, 6 and 7 polynomial fitting methods were separately used to design the cam curve, so that it could control the power of the polynomial while satisfying the local control conditions. When the parameters of the cam curve got optimized, constructed the cam mechanism into a three-dimensional model and carried out the dynamic simulation analysis. After comparing to the kinematic and dynamic …

Analysis Of Pressure Fluctuation Characteristics Of The Flow Field In The Cycloid Rotor Oil Pump, Wang Wen, Li Wenke, He Shanghong, Liu Guangming

Food and Machinery

In order to investigate the characteristics of pressure fluctuation in cycloid rotor oil pump, based on RNG k-ε turbulence model and moving mesh, the transient flows for different operating conditions in cycloid rotor pump were investigated numerically and the computational results of flow and efficiency were obtained. The pressure fluctuation at different monitoring points were measured and analyzed in time and frequency domains. The results show that the pressure fluctuation of the flow field in the cycloid rotor oil pump is periodical obviously, the amplitude of pressure fluctuation is smaller in the oil inlet chamber and decreases gradually along the …

A Research On Uniform Motion Of Sliding Block In Slider-Crank Mechanism, Li Haoxuan, He Hengjian, Liu Haonan, Li Yuelin

Food and Machinery

A variable crank length device with cam slot was added to the slide mechanism for achieving the uniform motion of the slider in the working stroke. The kinematics and dynamics analysis of this mechanism was carried out by using complex vector method and dynamic statics theory. Finally, the motion module of SolidWorks was used for the Motion analysis of this mechanism, and the motion characteristics and dynamic characteristics of the mechanism were obtained. The results showed that it was feasible to achieve uniform motion of slider in variable length crank slider mechanism.

Research On Food Packaging Design Based On Multidimensional Constraints, He Xuemei, Cao Tinglei, Lu Danqiong

Food and Machinery

Through analysis and research, this paper proposed a multidimensional constraint design model for food packaging, and clarified its advantages to the traditional packaging design. The model was built from the three dimensions of demand constraints, goal constraints, and resource constraints. Through the researches of user requirements, market requirements, first party requirements, resource limitations, etc., the corresponding design mapping domains under the relevant constraints were obtained, to clarify the elements of food packaging design, color, graphics, specifications, style, culture, and completed the design ultimately. The feasibility of the method and the model was verified by the actual case of the Qian …

Effect Of Different Tipping Glue And Tipping Paper On The Permeability Of Glue, Zhang Jing, Ma Xiaowei, Feng Xin, Qu Guofu, Wang Dongfei, Wang Jianmin

Food and Machinery

In order to evaluate the effect of glue penetration on cigarette filter ventilation rate, and provide the basis for tipping glue and tipping paper selection, using the PVC rectangular pore pressure plate control the area of the original coating area, calculate the penetration area of coloring glue using pixel method, estsblished a method to measure the natural permeability of the tipping glue, and the natural permeability of 2 kinds of tipping glue on 12 kinds of tipping paper was measured and analyzed. The results showed that: ① the natural permeability of 2 kinds of tipping glue on 12 kinds of …

Research On Experiential Food Package Design From The Perspective Of Body Perception, Tang Xinxing, Sun Xiangming, Gu Xiaofeng

Food and Machinery

Analyzed the neglects of body perception on current food package design, emphasized the significance of the research on experiential food package design and through practical examples explained the five types of experiential food package, which are sensory package, sustainable package, interesting package, situational package and emotional package.

Application Of Regional Cultural Symbol Translation In Design Of Nianli Food Package In Western Guangdong, Yan Li, Jiang Leige

Food and Machinery

Yuexi Nianli food packages have demands of blessing culture, the diet culture and regional culture, through analyzing the history and culture, the specific form and the humanities spirit in local cultural, refined the culture symbol elements in west of Guangdong. With the help of regional cultural symbol of translation technique, took the concept of Yuexi Nianli’s packaging design system: expressing of advancing with The Times’ spirit of “simple modelling＋ingenious structure＋AR technology applications”; conveying of the low-key and pragmatic attitude of “local materials＋green processing technology”; transmitting of the hope of “auspicious patterns and lucky colors”, aimed to promote the inheritance and …

Design Of Hickory Nuts Shell And Kernel Sorting System Based On Fuzzy Clustering Algorithm, Wang Tianyu, Cao Chengmao, Xie Chengjian, Li Zheng

Food and Machinery

In order to solve the problem of hickory nuts’ material mixing which was difficult to screen after breaking the shells, an image processing algorithm for hickory nuts color and texture selection was proposed based on fuzzy c-means clustering algorithm. The program with user interface for the separation of hickory nuts’ shells and kernels was developed on the Labview software platform. By extracting the characteristic data the hickory nuts with separation substances value of hue, saturation, value, angular second moment, entropy, inverse different moment and correlation, building a sample set of characteristic data, optimal data subset is preferred in accordance with …