Food Science Commons^™

Evaluation Of Inhibitory Activity Of A Polyoxometallate On Polyphenol Oxidase And Its Application In The Preservation Of Litopenaeus Vannamei, Fang Jia-Qi, Han Qing, Ma Yang-Yu, Wang Fang, Li Li-Li, Li Wen-Jie, Wang Li

Food and Machinery

Objective: To design new and effective polyphenol oxidase （PPO） inhibitors and expand their application in the field of aquatic product preservation. Methods: The synthesized H₈[P₂Mo₁₇Ni（OH₂）O₆₁] （P₂Mo₁₇Ni） structure was characterized by ultraviolet and infrared spectrum. The inhibitory effect, type and mechanism of P₂Mo₁₇Ni on the relative activity of mushroom PPO in vitro was clarified. Then, the preservation effects of P₂Mo₁₇Ni on Litopenaeus vannamei Boone were evaluated through studying the index change of shrimps treated with P₂Mo_{17 …}

Comparative Nutritional Assessment And Metabolomics Of A Wrky Rice Mutant With Enhanced Germination Rates, Santiago Bataller, Anne J. Villacastin, Qingxi J. Shen, Christine Bergman

Life Sciences Faculty Research

Rice is the primary staple food for half the world’s population. Climate change challenges and food insecurity supports the need for rice with agronomically advantageous traits. We report on a transposon insertional rice mutant with enhanced germination rates. This trait is advantageous for rice growth in limited water regions and to reduce yield constraints caused by weed and bird competition. Evaluations of vital nutritional components, compositional analysis, and comparative metabolomics on threshed grain samples are performed, as these assays are those used to assess the safety of foods from genetically modified crops. Compared with the wild type (cv. Nipponbare), oswrky71 …

Fatty Acid, Carotenoid And Fat-Soluble Vitamin Composition Of Multispecies Swards Grown In Ireland—Implications For A Sustainable Feed In Dairy Farming, Samuel Rapisarda, Graham O'Neill, Nissreen Abu-Ghannam

Articles

Although traditional ryegrass pastures are still commonly used, they require intense management and input and do not perform well during the summer months. Multispecies systems have been recognised as more sustainable, needing less fertiliser and being tolerant to drought and heat. While data on monoculture system fat composition exist, information for multispecies is scarce. The present study compared the fatty acid (FA), carotenoid and fat-soluble composition of a multispecies system (consisting of perennial ryegrass, timothy, white clover, red clover, chicory and plantain) (MULTI) with two other conventional grazing systems (monoculture perennial ryegrass (PRG) and a binary mixture of perennial ryegrass …

The Science Of Baking Bread, Admin Stem For Success

STEM for Success Showcase

Activity plan to teach biology and chemistry using a lesson on the baking of bread.

Fusarium Species Structure In Nebraska Corn, Yuchu Ma

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Fusarium species are known to infect corn and cause significant yield losses and mycotoxin contamination worldwide. In this study, we investigated the diversity of Fusarium species infecting corn in Nebraska and their potential to produce fumonisins and trichothecenes. A total of 259 Fusarium isolates were collected from different corn tissues (ear, stalk, and root), revealing a significant association between the various Fusarium species complexes and different plant parts (p < 0.05). Fusarium incarnatum-equiseti species complex (FIESC) was the most widespread and abundant, followed by the Fusarium sambucinum (FSAMSC) and Fusarium fujikuroi species complexes (FFSC). In the subsequent analysis, we investigated the mycotoxin …

Effects Of Folding On Laminated Dough, Shelby Thorpe, Sarah Moore, Heather Burr, Colton Coombs

Student Research Symposium

The purpose of this experiment is to determine the effect on volume, flakiness, and air cell size and amount in basic croissants when the dough is folded less than and more than what’s currently considered standard. The process of dough lamination, or the creation of alternating layers of dough and fat, is a technique used in various pastries. The layer of fat, often butter or margarine, creates an impenetrable barrier to the steam, or water vapor, produced by surrounding dough layers. This facilitates lift within the pastry and while many types don’t contain yeast, lamination becomes crucial to the finalized …

How Does Protein Content Of Flour Affect Sourdough Bread?, Julie Eggett, Callie Goble, Whitney Nelson

Student Research Symposium

The goal of this experiment was to evaluate if the micro-differences in protein content of bread flour made a difference in volume, air cell size, appearance, texture and flavor of sourdough bread. The flour used in this experiment included Bob’s Red Mill Artisan Bread Flour, Big J Mill Golden Loaf Bread Flour, Gold Medal Bread Flour. The protein contents were 13%, 12.7%, and 10.5%, respectively. The bread was baked using a standard recipe with only the brand of bread flour varying from sample to sample. The samples were then objectively and subjectively measured. Volume of bread samples of equal weight …

Assessing How Various Cooking Methods Influence Several Physical Qualities Of Chicken Breasts, Matthew Hulse, Jinjer Smith, Sami Allen, Melody Loutzenhiser

Student Research Symposium

Juiciness and tenderness are two of the characteristics which define meat quality and acceptability for a consumer. The purpose of this experiment is to determine the effect on moisture loss, tenderness, chewability, taste, aroma, color, and texture on chicken breast when cooked using different methods such as: sous-vide (water bath), baking, air-frying, and pan-frying. Killing harmful organisms by cooking is necessary for meat, and these different methods result in different moisture retention and texture of the product. The intention of this experiment is to equip consumers with the information they need to choose which cooking method best aligns with their …

Thematic Analysis Of Food Safety Literature: The Fda Food Code And State Legislation, Sabrina Nicole Backlund

Doctoral Dissertations and Projects

One of the most important purposes of communication is to warn people of environmental dangers. More specifically, communication can identify risks associated with food safety. The government and food safety experts are to provide information on these dangers. This study aims to determine whether these entities use consistent language to communicate these dangers. The reader will first take a journey through relevant communication concepts and an introduction to food safety. This qualitative applied communication analysis uses MAXQDA software to ascertain similarities in word choice between the Food and Drug Administration (FDA) food code and state legislative material. This comparison uses …

Dairy Digest 2023 : Live Like Someone Left The Gate Open, South Dakota State University Dairy Club

Dairy Digest

This is the 2023 Dairy Digest. The Diary Digest is published annually by the South Dakota State University Dairy Club. The Digest contains information and news about the activities and members of the SDSU Dairy Club for the academic year 2022 - 2023.

Development Of Value-Added Butter By Incorporating Whey Protein Hydrolysate-Encapsulated Probiotics, Kritika Gaba, Sanjeev Anand, Athira Syamala

Dairy Science and Food Science Faculty Publications: 2022 to Present

The probiotic foods market is growing exponentially; however, probiotics’ survivability and interaction with product attributes pose major challenges. A previous study of our lab developed a spray-dried encapsulant utilizing whey protein hydrolysate-maltodextrin and probiotics with high viable counts and enhanced bioactive properties. Viscous products such as butter could be suitable carriers for such encapsulated probiotics. The objective of the current study was to standardize this encapsulant in salted and unsalted butter, followed by storage stability studies at 4 ◦C. Butter was prepared at a lab-scale level, and the encapsulant was added at 0.1% and 1%, followed by physiochemical and microbiological …

Growing South Dakota (Spring 2023), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 Developing Climate-Smart Beef and Bison Commodities
[Page] 5 Joseph Cassady Leads CAFES as New Dean
[Page] 7 Inaugural Class of Klingbeil Scholars
[Page] 9 Jackrabbits to Future Veterinarians
[Page] 11 Protecting South Dakota’s Grasslands
[Page] 13 Growing Youth Programs
[Page] 15 Partnerships for Positive Growth
[Page] 17 Building Highly Effective Boards
[Page] 19 SDSU Extension Podcasts
[Page] 21 Providing Good Food for All
[Page] 23 Improving Seed Varieties
[Page] 25 CAFES Endowed Positions
[Page] 29 Jackrabbits Now and Then

Bioavailability Of Citrulline In Watermelon Flesh, Rind, And Skin Using A Human Intestinal Epithelial Caco-2 Cell Model, Inah Gu, Olugbenga Balogun, Cindi Brownmiller, Hye Won Kang, Sun-Ok Lee

Food Science Faculty Publications and Presentations

Watermelon produces many byproducts (watermelon rind and skin) even though those components contain various bioactive compounds, including citrulline. This study evaluated the citrulline concentration, total phenolic content, and antioxidant activity (DPPH and FRAP assays) of different parts of watermelon and investigated the bioavailability of citrulline from different parts of watermelon using an in vitro human intestinal epithelial Caco-2 cell monolayer model. Solid-phase extracted watermelon flesh, rind, and skin samples were treated on a Caco-2 cell monolayer for 1, 2, and 4 h. The collected basolateral solution at each time point was analyzed for the percentage of citrulline transport. Watermelon flesh …

Effect Of The Physical Fibrillated Sweet Potato (Ipomoea Batatas) Stem On The Plant-Based Patty Analogues, Suyoon Lee, You Young Lee, Yang Kim, Seung Hwan Ham, Min Gyu Lee, Jungwoo Hahn, Young Jin Choi

Faculty, Staff and Student Publications

In this study, a dietary fiber extracted from sweet potato stems (Ipomoea batatas, PS) was evaluated for its ability to improve the quality of vegetable patty analogues. A patty analogues containing 0–50 wt% dietary fiber were prepared to analyze the utilized dietary fiber's performance. To evaluate the manufactured patty analogues, texture profile analysis, color analysis, emulsion stability, and microstructural analysis were conducted. As the PS increased, the hardness decreased, while the total expressible fluids tended to increase. The color analysis revealed that the a* value, which represents red, declined as the PS content increased, and heterogeneous colors showed …

Effect Of Uv-C Light Against Listeria Monocytogenes On Hydroponically Grown Lettuce And Its Effect On Quality, Ivannova Lituma

LSU Master's Theses

Controlled agriculture environments like hydroponic systems are a growing industry. However, studies have shown potential of microbial growth in hydroponic systems, highlighting the need for decontamination techniques to prevent the risk of foodborne diseases. This study evaluated the effect of UV-C light against Listeria monocytogenes on hydroponically grown lettuce and seeds. Lettuce leaves were spot inoculated (100μL) with Listeria monocytogenes cocktail and exposed to UV-C light (0.28mW/cm2) for 10 to 50 seconds in 10-second increments and 1 to 5 minutes in 1- minute increments. UV treated leaves were stored at 4C° and visually inspected for 5 days. Additionally, the smooth …

Effects Of Vacuum Packaging And Chilling Storage On The Microbiological Changes Of The Superheated Steam Dried Sardines, Prissillah Antelm, Ibrahim Juma, Leonard M.P. Rweyemamu, Lillian Daniel Kaale

Tanzania Journal of Science

Fish are important sources of protein and micronutrients for combating malnutrition. Since fish are highly perishable, methods for processing and preserving them should be the main focus to increase their shelf life and maintain their quality. The present study assessed the effectiveness of the superheated steam dryer (SSD) in processing sardines, and examined the effects of packaging and storage conditions on their microbiological quality. Sardines collected from the Mafia Island were dried in SSD, air-packed and stored at room temperature (AR), air-packed and stored at chilling temperature (AC), vacuum-packed and stored at room temperature (VR), and vacuum-packed and stored at …

Household And Community Factors Affecting Nutritional Status Of Under-Five Children (6-59 Months) In Gairo District Using Composite Index Of Anthropometric Failure, Tausi M. Mohamed, Cornelio N. Nyaruhucha

Tanzania Journal of Science

In Tanzania, where the majority of under-five children are affected, undernutrition is still a significant public health concern. The study aimed at assessing the prevalence of undernutrition and its determinants among under-five children in Gairo District using a Composite Index of Anthropometric Failure (CIAF). A household-based cross-sectional study was employed involving 300 under-five children with their mothers/caregivers in three wards in the Gairo District. Data collection on socio-demographics, dietary diversity, and healthcare factors were collected by interviewer-based semi-structured questionnaires. Anthropometric measurements using standard procedures were performed to collect anthropometric data. Odds ratios with a 95% confidence interval and p-value ≤ …

Evaluation Of Free Radical Scavenging  Activity Of Sauce And Formulation Of Tamarindus Indica (L.) And Prunus Domestica (L.) Fruits, Zulnoreen Sarfaraz, Mehmooda Munazir, Mubashrah Munir, Asma Ahmed, Rehana Badar, Ayesha Tahir

Journal of Bioresource Management

The formulation and sauce of Tamarindus indica  (L.) and Prusnus  dometica (L.) are very popular recipes across globe. But, not even a single study existed on free-radical scavenging activity (FRSA) of afore-mentioned recipes. So, the present study was designed to bridge this gap of information. The FRSA of individual samples were also determined and compared with that of combinations of both. The highest value for FRSA among all samples was that of T. indica (158.9732 ± 12.658 % at 100 μg ml^-1). It was followed by that of individual extract of P. domestica fruit (96.647 ± 0.554 %). …

Design, Biological Evaluation, And Computer-Aided Analysis Of Dihydrothiazepines As Selective Antichlamydial Agents, Luana Janaína De Campos, Mohamed A. Seleem, Jiachen Feng, Kelly Mari Pires De Oliveira, João Víctor De Andrade Dos Santos, Shivdeep Hayer, Jonathan B. Clayton, Sharvath Kathi, Derek J. Fisher, Scot P. Ouellette, Martin Conda-Sheridan

Department of Food Science and Technology: Faculty Publications

Chlamydia trachomatis (CT) causes the most prevalent sexually transmitted bacterial disease in the United States. The lack of drug selectivity is one of the main challenges of the current antichlamydial pharmacotherapy. The metabolic needs of CT are controlled, among others, by cylindrical proteases and their chaperones (e.g., ClpX). It has been shown that dihydrothiazepines can disrupt CT-ClpXP. Based on this precedent, we synthesized a dihydrothiazepine library and characterized its antichlamydial activity using a modified semi-high-throughput screening assay. Then, we demonstrated their ability to inhibit ClpX ATPase activity in vitro, supporting ClpX as a target. Further, our lead …

Effect Of Type Of Mulch And Biological Soil Amendments Of Animal Origin (Bsaao) On Microbial Food Safety Risk On Cucumbers And Carrots Irrigated With Contaminated Or Chlorinated Water, Juan Fernando Moreira Calix

LSU Doctoral Dissertations

A comprehensive understanding of the effect biological soil amendments of animal origin and physical soil amendments have on produce contamination is important for developing strategies to mitigate this risk. Our studies investigated the persistence of generic E. coli, E. coli O157:H7 and Salmonella spp. in soil and produce surfaces with different BSAAO and mulch applications, as well as the effectiveness of chlorine treatments in reducing enteric bacteria in the field. Cucumbers harvested from plots with paper mulch had the highest levels of E. coli (3.76 Log CFU/cm²) while samples from maize mulch had the lowest levels (1.68 …

Multilingual Zambia - Language Issues In Primary/Secondary Schools Of The Eastern/Southern Provinces, Kenzie Steiner

NUTR/GLST 498b: Global Research Experiences in Nutrition and Health

Introduction: Zambia is a multilingual country that uses 8 different languages for instruction including English and 7 other indigenous languages.

Methods: Survey research conducted between May-June 2022 on 6-7th graders within 9 Zambian schools. Classroom observations made and teachers interviewed.

Results: In Eastern and Southern Provinces, Zambian teachers speak an average of 5 languages while students speak an average of 2. Both teachers and students say English remains the most important language followed by first languages.

Conclusion: Continued research on language-in-education policies and impacts on student performance must be conducted if “One Zambia, One Nation” is meant to promote all …

Evaluating Sensory Quality, Willingness To Try, Risk And Product Appropriateness Of Seafood Byproducts, Silvia Jessenia Murillo Miguez

LSU Master's Theses

Valorization of food wastes/byproducts has become a major subject of research to increase sustainability of the global food supply chain. Seafood industries generate a substantial quantity of byproducts. An online survey was used to evaluate Willingness-to-try (WTT), the effect of a Consumer Safety Statement (CSS) on WTT, perceived risks and product appropriateness associated with seafood byproducts in food as influenced by gender and race. Based on the finding from this survey, a clean label catfish bone powder (CBP) was developed and used for breaded catfish sticks which were evaluated for sensory liking, emotional profile, and purchase intent. Previous consumers of …

Polyvinylpyrrolidone-Passivated Fluorescent Iron Oxide Quantum Dots For Turn-Off Detection Of Tetracycline In Biological Fluids, Sri Sudewi, Lutfi Chabib, Muhammad Zulfajri, Gangaraju Gedda, Genin Gary Huang

Journal of Food and Drug Analysis

Tetracycline is an antibiotic that has been prescribed for COVID-19 treatment, raising concerns about antibiotic resistance after long-term use. This study reported fluorescent polyvinylpyrrolidone-passivated iron oxide quantum dots (IO QDs) for detecting tetracycline in biological fluids for the first time. The as-prepared IO QDs have an average size of 2.84 nm and exist a good stability under different conditions. The IO QDs’ tetracycline detection performance could be attributed to a combination of static quenching and inner filter effect. The IO QDs displayed high sensitivity and selectivity toward tetracycline and achieved a good linear relationship with the corresponding detection limit being …

Chemical Transformation Of Cannabidiol Into Psychotropic Cannabinoids Under Acidic Reaction Conditions: Identification Of Transformed Products By Gc-Ms, Minsun Jeong, Sangin Lee, Chaeyoung Seo, Eunjeong Kwon, Soohyang Rho, Mansoo Cho, Moon Yeon Kim, Wonwoong Lee, Yong Sup Lee, Jongki Hong

Journal of Food and Drug Analysis

Recently, cannabidiol (CBD), one of the major components of the Cannabis species, has been a focus in the cannabis industry due to its various pharmacological effects. Interestingly, CBD can be converted into several psychoactive cannabinoids, such as 9-tetrahydrocannabinol (Δ⁹-THC) and its structural isomers, under acidic reaction conditions. In this study, chemical transformation of CBD in ethanol solution was conducted with variation in pH at 2.0, 3.5, and 5.0 by addition of 0.1 M hydrochloric acid (HCl). These resulting solutions were derivatized with trimethylsilyl (TMS) reagent and analyzed using GC/MS-scan mode. Time profiles of CBD degradation and transformation of …

Development Of A Metabolomics-Based Data Analysis Approach For Identifying Drug Metabolites Based On High-Resolution Mass Spectrometry, Hsiao-Hsien Ting, Yi-Shiou Chiou, Tien-Yi Chang, Guan-Yu Lin, Pei-Jhen Li, Chia-Lung Shih

Journal of Food and Drug Analysis

A metabolomics-based approach to data analysis is required for drug metabolites to be identified quickly. This study developed such an approach based on high-resolution mass spectrometry. Our approach is a two-stage one that combines a time-course experiment with stable isotope tracing. Pioglitazone (PIO) was used to improve glycemic management for type 2 diabetes mellitus. Consequently, PIO was taken as a model drug for identifying metabolites. During Stage I of data analysis, 704 out of 26626 ions exhibited a positive relationship between ion abundance ratio and incubation time in a time-course experiment. During Stage II, 25 isotope pairs were identified among …

Untargeted Metabolomics Analysis Assisted By Signal Selection For Comprehensively Identifying Metabolites Of New Psychoactive Substances: 4-Meo-Α-Pvp As An Example, Hsin-Yi Wu, Yuan-Chih Chen, Jing-Fang Hsu, Hsiang-Ting Lu, Yu-Yi Pan, Mi-Chia Ma, Pao-Chi Liao

Journal of Food and Drug Analysis

New psychoactive substances (NPS) have been rapidly emerged as legal alternatives to controlled drugs, which raised severe public health issue. The detection and monitoring of its intake by complete metabolic profiling is an urgent and vital task. Untargeted metabolomics approach has been applied for several NPS metabolites studies. Although the number of such works is relatively limited but with a rapidly growing need. The present study aimed to propose a procedure that includes liquid chromatography high-resolution mass spectrometry (LC-HRMS) analysis and a signal selection software, MetaboFinder, programed as a web tool. The comprehensive metabolites profile of one kind of NPS, …

Guilu Erxian Jiao Enhances Protein Synthesis, Glucose Homeostasis, Mitochondrial Biogenesis And Slow-Twitch Fibers In The Skeletal Muscle, Wei-Yu Fang, Wan-Hsuan Chang, Yi-Hong Tsai, Hung-Te Hsu, Fang-Rong Chang, Chih-Lung Lin, Yi-Ching Lo

Journal of Food and Drug Analysis

Guilu Erxian Jiao (GEJ) is a commonly used nutritional supplement due to its rich content of amino acids. It is also a traditional herbal medicine for improving degenerative joint. This study aimed to investigate the effect and mechanism of GEJ water extract (GEJ-WE) on skeletal muscle in C2C12 myotubes and C57BL/6J mice. Analysis of GEJ-WE were performed by high-performance liquid chromatography fingerprinting with chemical standards. Protein expression, mRNA level, glycogen content, mitochondria activity and ATP level were evaluated by western blots, real-time PCR, PAS staining, MTT and ATP bioluminescence assay, respectively. Skeletal muscle strength was evaluated by grip strength. Skeletal …

High-Resolution 1h Nmr Analysis Of Continuous And Discontinuous Thermo-Oxidative Susceptibility Of Culinary Oils During Frying At 180℃, Gilbert Ampem, Adam Le Gresley, Martin Grootveld, Declan P Naughton

Journal of Food and Drug Analysis

Lipid oxidations products (LOPs) are reactive mutagenic and carcinogenic species known to be generated in thermally stressed culinary oils. Mapping the evolution of LOPs in culinary oils exposed to standard frying practices – both continuous and discontinuous thermo-oxidation – at 180℃ is vital to our understanding of these processes, and to the development of scientific solutions for their effective suppression. Modifications in the chemical compositions of the thermo-oxidised oils were analysed using a high-resolution proton nuclear magnetic resonance (¹H NMR) technique. Research findings acquired showed that polyunsaturated fatty acid (PUFA)-rich culinary oils were the most susceptible to thermo-oxidation. …

Investigation Of Monacolin K, Yellow Pigments, And Citrinin Production Capabilities Of Monascus Purpureus And Monascus Ruber (Monascus Pilosus), Tzu-Shing Lin, Shih-Hau Chiu, Chien-Chi Chen, Chih-Hui Lin

Journal of Food and Drug Analysis

Red mold rice (RMR) is a traditional Chinese medicine prepared using Monascus fermentation. M. ruber (pilosus) and M. purpureus have a long history of use as food and medicine. As an economically important starter culture, the relationship between the taxonomy of Monascus and production capabilities of secondary metabolites is crucial for the Monascus food industry. In this study, monacolin K, monascin, ankaflavin, and citrinin production by M. purpureus and M. ruber were genomically and chemically investigated. Our findings suggest that M. purpureus can produce monascin and ankaflavin in a correlated manner, whereas M. ruber produces monascin with minimum …

Determination Of 24 Sulfonamide Antibiotics In Instant Pastries By Modified Quechers Coupled With Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry, Shuo Li, Can Zhang, Hui-Xin Tang, Yue Gu, Ai-Jing Guo, Ke Wang, Kao-Qi Lian

Journal of Food and Drug Analysis

There were few reports about antibiotic residues in egg-containing products. In the study, an effective method for the simultaneous determination of 24 sulfonamide antibiotics in two instant pastries based on a modified QuEChERS sample preparation technique coupled with ultra performance liquid chromatography-tandem mass spectrometry was developed. The results show that the average recoveries of the SAs at 5, 10, and 50 µg·kg^-1 levels were 67.6%-103.8%, with relative standard deviations (RSD) of 0.80-9.23%. The limit of detections (LODs) and limit of quantitations (LOQs) were 0.01-0.14 µg∙kg^-1 and 0.02-0.45 µg∙kg^-1, respectively. This method was suitable for analysis of …