Meat Science Commons^™

Beta-Adrenergic Agonists Increase Maximal Output Of Oxidative Phosphorylation In Bovine Satellite Cells, Renae L. Sieck, Leah K. Treffer, Martonio Ponte Viana, Oleh Khalimonchuk, Ty B. Schmidt, Dustin T. Yates, Jessica L. Petersen

Department of Animal Science: Faculty Publications

Beta-adrenergic agonists (βAA), Ractopamine HCl (RH) and Zilpaterol HCl (ZH), are FDAapproved supplements utilized in pigs and cattle to improve growth performance, carcass weight, and longissimus muscle area (Arp et al., 2014; Lean et al., 2014). Previous studies within our group have focused on understanding molecular changes in skeletal muscle due to βAA supplementation. This work has shown that βAA supplementation increases glucose oxidation in muscle from thermoneutral and heat-stressed lambs (Barnes et al., 2019) and in rat skeletal muscle stimulated with ZH (Cadaret et al., 2017). Skeletal muscle transcriptomics of lambs supplemented ZH revealed upregulation of genes associated with …

Heat Stress And Β-Adrenergic Agonists Alter The Adipose Transcriptome And Fatty Acid Mobilization In Ruminant Livestock, Rachel R. Reith, Renae L. Sieck, Pablo C. Grijalva, Erin M. Duffy, Rebecca M. Swanson, Anna M. Fuller, Kristin A. Beede, Joslyn K. Beard, Duarte E. Diaz, Ty B. Schmidt, Dustin T. Yates, Jessica L. Petersen

Department of Animal Science: Faculty Publications

Growth and feed efficiency of cattle are improved by supplementation with the beta-adrenergic agonists (βAA), ractopamine hydrochloride (RH; β₁AA) or zilpaterol hydrochloride (ZH; β₂AA) (Elam et al., 2009). βAA supplementation alters adipose deposition by inhibiting fatty acid biosynthesis and promoting lipolysis of stored triacylglycerols into free fatty acids (FFAs) (Johnson et al., 2014). However, β₂ adrenoceptors (βAR) desensitize with chronic activation (Re et al., 1997); supplementation is thus limited to the last 20 to 40 d of feeding.

The annual economic impact of heat stress (HS) has been estimated to exceed $2.4 billion (St-Pierre et …

Bovine Respiratory Disease Influences On Nutrition And Nutrient Metabolism, Clinton R. Krehbiel

Department of Animal Science: Faculty Publications

• Inflammation caused by bovine respiratory disease (BRD) continues to be one of the greatest challenges facing beef cattle producers and feedlot managers.

• BRD results in decreased intake, daily gain, and feed efficiency in feedlot calves, decreasing growth rate and increasing required days on feed.

• Morbidity caused by BRD has been associated with decreased hot carcass weight and poor carcass characteristics.

• Acute phase protein production in the liver, initiated by proinflammatory cytokines, may shift the priority for amino acid and energy use by the host animal during periods of sickness.

• Nutrient requirements for stressed calves seem …

Differential Longitudinal Establishment Of Human Fecal Bacterial Communities In Germ-Free Porcine And Murine Models, Nirosh Aluthge, Wesley A. Tom, Alison Bartenslager, Thomas E. Burkey, Phillip S. Miller, Kelly D. Heath, Craig Kreikemeier-Bower, Hatem Kittana, Robert Schmaltz, Amanda Ramer-Tait, Samodha Fernando

Department of Animal Science: Faculty Publications

The majority of microbiome studies focused on understanding mechanistic relationships between the host and the microbiota have used mice and other rodents as the model of choice. However, the domestic pig is a relevant model that is currently underutilized for human microbiome investigations. In this study, we performed a direct comparison of the engraftment of fecal bacterial communities from human donors between human microbiotaassociated (HMA) piglet and mouse models under identical dietary conditions. Analysis of 16S rRNA genes using amplicon sequence variants (ASVs) revealed that with the exception of early microbiota from infants, the more mature microbiotas tested established better …

Age Related Extracellular Matrix And Interstitial Cell Phenotype In Pulmonary Valves, Shaohua Wu, Vikas Kumar, Peng Xiao, Mitchell Kuss, Jung Yul Lim, Chittibabu Guda, Jonathan Butcher, Bin Duan

Department of Animal Science: Faculty Publications

Heart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 …

Susceptibility Of Swine Cells And Domestic Pigs To Sars-Cov-2, David A. Meekins, Igor Morozov, Jessie D. Trujillo, Natasha N. Gaudreault, Dashzeveg Bold, Mariano Carossino, Bianca L. Artiaga, Sabarish V. Indran, Taeyong Kwon, Velmurugan Balaraman, Daniel W. Madden, Heinz Feldmann, Jamie Henningson, Wenjun Ma, Udeni B. R. Balasuriya, Juergen A. Richt

Faculty Publications

No abstract provided.

Artificial Intelligence And Covid-19: Deep Learning Approaches For Diagnosis And Treatment, M. B. Jamshidi, A. Lalbakhsh, J. Talla, Z. Peroutka, F. Hadjilooei, P Lalbakhsh, M. Jamshidi, L. La Spada, M. Mirmozafari, M. Dehghani, A. Sabet, Sa. Roshani, So. Roshani, N. Bayat-Makou, B. Mohamadzade, Z. Malek, A. Jamshidi, S. Kiani, H. Hashemi-Dezaki, W. Mohyuddin

Faculty Publications

COVID-19 outbreak has put the whole world in an unprecedented difficult situation bringing life around the world to a frightening halt and claiming thousands of lives. Due to COVID-19 & x2019;s spread in 212 countries and territories and increasing numbers of infected cases and death tolls mounting to 5,212,172 and 334,915 (as of May 22 2020), it remains a real threat to the public health system. This paper renders a response to combat the virus through Artificial Intelligence (AI). Some Deep Learning (DL) methods have been illustrated to reach this goal, including Generative Adversarial Networks (GANs), Extreme Learning Machine (ELM), …

Effect Of Different Fat Sources And Vitamin E Isoforms/ Levels On Carcass Characteristics, Meat Quality, And Belly/Bacon Characteristics Of Pigs Grown To Heavy Slaughter Weights (>150kg), Marlee Kelley

Theses and Dissertations--Animal and Food Sciences

Two separate studies were conducted to evaluate the potential interaction of fat sources and vitamin E (VE) on heavy slaughter weights. In Study 1, a total of 64 individually-fed pigs (28.41 ± 0.83 kg) were randomly assigned to 8 dietary treatments in a 4×2 factorial arrangement. Fat treatments included cornstarch (CS), tallow (TW), corn oil (CO), and coconut-oil (CN). VE treatments were dietary alpha-tocopheryl-acetate (ATA) at 11 and 200 ppm. In Study 2, a total of 72 individually fed pigs (28.55 ± 1.16 kg) were randomly assigned to 12 dietary treatments in a 2 × 6 factorial arrangement. Fat treatments …

The “Challenge" Of Depletion: Why The Oyster Fishery Is Not Self-Regulating, Eric N. Powell, John M. Klinck, Leanne M. Poussard

CCPO Publications

The possibility that the economics of the oyster fishery impose a self-limitation on overharvesting has been proffered on occasion. The inefficiency of harvesting by the fishery has been evaluated and estimates of the exploitation rate permissible under conditions of maximum sustainable yield have been obtained in previous studies. The question becomes to what extent does the inefficiency of harvest interact with the economics of the fishery to compromise ready detection of overfishing? This study explores the possibility that the constraint of economics on the fishery occurs at oyster exploitation rates that are higher than maximum sustainable yield, leading ineluctably to …

Growth And Performance Of Terminal Sired Calves Grazing Range Or Meadow Pasture, Robert L. Ziegler, Tonya L. Meyer, Jacki A. Musgrave, J. C. Macdonald, J. Travis Mulliniks, Rick N. Funston

Nebraska Beef Cattle Reports

Multiparous dams were assigned to be bred by artificial insemination or natural service to bulls with terminal traits. Additionally, the cow- calf pairs grazed upland range or sub- irrigated meadow from June 1 to weaning in November. Two weeks aft er weaning, calves entered the feedlot as calffeds. Natural service range calves had the lightest weaning weights, final live weights, and hot carcass weights. Additional days on feed may be required for natural service range calves to reach similar body weights and carcass characteristics as other treatments. Average daily gain and feed conversion was improved in calves that grazed range …

Evaluation Of Masters Choice Corn Silage On Growing Steer Performance, Jiehua Xiong, Bradley M. Boyd, Levi Mcphillips, Kyle Vosburgh, Galen E. Erickson

Nebraska Beef Cattle Reports

A growing study evaluated three corn silage hybrids on growing steer performance. Th e three hybrids were: a conventional hybrid- Farm Choice (CON) commonly grown in Eastern Nebraska which served as the control, Masters Choice hybrids MCT6365 RIB (MC1) selected to improve fi ber and starch digestion and MCT6733 GT3000 (MC2) that has been selected to improve fi ber digestion in cattle. Relative to CON, feeding hybrid MC1 resulted in similar DMI, but numerically increased ADG which significantly improved F:G compared to CON. Feeding MC2 led to greater DMI, similar ADG, and poorer (greater) F:G compared to CON. Feeding Masters …

Evaluation Of Level Of Milk Potential On Nutrient Balance In 2- And 4- Year- Old May- Calving Range Cows Grazing Sandhills Upland Range, J. Travis Mulliniks, Don C. Adams

Nebraska Beef Cattle Reports

A modeling study evaluated the effects of milk production level on nutrient balance in May- calving cows grazing Sandhills upland range during the breeding season. Forage quality of upland range peaks in June and steadily declines in July until November. With timing of forage quality decline and increasing nutrient demands due to lactation, cows were in a negative energy balance in late June and early July prior to deficiency of metabolizable protein. Supplementation to meet energy deficiencies in June and July and MP deficiencies in July with distiller grains that is high ruminally undegradable protein and high fiber energy may …

Evaluation Of Rup Content Of Nexpro Dried Distillers Grains Plus Solubles And Their Effect On Growing Calf Performance In Corn Silage Based Diets, Aksel R. Wiseman, Zachary E. Carlson, Levi J. Mcphillips, Galen E. Erickson, Andrea K. Watson, Scott L. Tilton

Nebraska Beef Cattle Reports

A growing study was conducted to evaluate the effect of supplementing NexPro, a high- protein dried distillers grains plus solubles (DDGS) from the FluidQuip MSC post- fermentation separation process, in a corn- silage based diet and to determine the rumen undegradable protein (RUP) content of NexPro. Three test proteins (NexPro, SoyPass, and soybean meal) and 4 inclusion levels (4.5, 9, 13.5, and 18%) were evaluated against a common control (0% test protein). Th ere were no differences in dry matter intake among treatments. Increasing inclusion of NexPro resulted in improved feed conversion and daily gain. SoyPass and soybean meal supplementation …

Forage Production And Calf Gains When Grazing Oats Following Corn Harvest, Mckenna M. Brinton, Benjamin H. Hansen, Kristen M. Ulmer, Zachary E. Carlson, F. Henry Hilscher, Mary E. Drewnoski, Jim C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A 4 yr. study was conducted to evaluate forage yield and grazing potential of double cropped annual forages following corn silage or high- moisture corn harvest. An irrigated field in a corn- soybean rotation was split in half and harvested as either corn silage or high- moisture corn, and crops were sampled to determine any effects on subsequent yield due to cover and grazing. Over the four years, steers grazing oats aft er corn silage harvest gained an average of 2.35 lb/d, while those grazing corn residue and oats aft er high- moisture corn harvest averaged 1.28 lb/d. Average oat …

Evaluation Of Fluidquip Fiber Stream Dried Distillers Grains Plus Solubles On Performance And Carcass Characteristics In Finishing Diets, Aksel R. Wiseman, Bradley M. Boyd, Levi J. Mcphillips, Galen E. Erickson, Andrea K. Watson, Scott L. Tilton

Nebraska Beef Cattle Reports

A finishing study was conducted to evaluate the effect of feeding dried distillers grains plus solubles (DDGS) from the MSC- fi ber stream of the FluidQuip post- fermentation fiber separation process compared to conventional DDGS at two inclusion levels (20% and 40% of diet dry matter). Inclusion of DDGS from MSC or conventional processing methods resulted in increased dry matter intake and daily gain. Despite increased gain, feed conversion tended to be worse for MSC DDGS. Daily gain tended to respond quadratically with increasing inclusion of conventional DDGS with gain increasing from 0 to 20% inclusion, but decreasing from 20 …

Statistics Used In The Nebraska Beef Cattle Report And Their Purpose

Nebraska Beef Cattle Reports

Th e purpose of beef cattle and beef product research at UNL is to provide reference information that represents the various populations (cows, calves, heifers, feeders, carcasses, retail products, etc) of beef production. Obviously, the researcher cannot apply treatments to every member of a population; therefore he/she must sample the population. Th e use of statistics allows the researcher and readers of the Nebraska Beef Cattle Report the opportunity to evaluate separation of random (chance) occurrences and real biological effects of a treatment. Following is a brief description of the major statistics used in the beef report. For a more …

Impact Of Myoglobin Oxygenation State On Color Stability Of Frozen Beef Steaks, Morgan L. Henriott, Felipe A. Ribeiro, Nicolas J. Herrera, Kellen B. Hart, Nicolas A. Bland, Chris Calkins

Nebraska Beef Cattle Reports

The objective of this study was to determine the impacts of myoglobin oxygenation level and frozen storage duration on frozen beef color. Strip loins were wet- aged for 4 or 20 days and were fabricated into steaks that were assigned a myoglobin oxygenation level (highly oxygenated, lowly oxygenated, or deoxymyoglobin) and packaging film (impermeable or permeable). Steaks were then frozen for 0, 2, 4, or 6 months of storage and analyzed for various beef color measurements. Highly oxygenated steaks had greater a* values (redness) and percent oxymyoglobin compared to the other treatments. Frozen storage beyond 4 months and oxygen impermeable …

Dose Titration Of Wet Distillers Grains Plus Solubles Replacing Syngenta Enogen Feed Corn And Interaction Between Corn Type And Distillers Inclusion, Mckenna M. Brinton, Bradley M. Boyd, Brianna B. Conroy, Hannah C. Wilson, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

incomplete 2×4 factorial finishing study evaluated the effect of corn hybrid fed as dry- rolled corn, and inclusion level of wet distillers grains plus solubles on finishing performance of yearling steers. Th e two hybrids included a conventional commercial corn and Syngenta’s Enogen Feed Corn which contains an alpha amylase enzyme trait. Diets contained 0, 15, 30, or 45% with Syngenta Enogen Feed Corn and 0 or 30% wet distillers grains plus solubles in control corn diets. Increasing wet distillers grains plus solubles with Syngenta Enogen Feed Corn linearly increased hot carcass weight, dry matter intake, and average daily gain, …

Nutrient Digestibility Of Condensed Algal Residue Solubles In Beef Cattle Fishing Diets, Mitchell M. Norman, Hannah C. Wilson, Andrea K. Watson, Galen E. Erickson, Jonathan W. Wilson

Nebraska Beef Cattle Reports

Condensed algal residue solubles (CARS) were evaluated in finishing cattle diets. Six treatments were evaluated (2 × 3 factorial arrangement), CARS inclusion in the diet at 0, 5, or 10% of diet dry matter with 0 or 20% wet distillers grains. Th e remainder of the diets consisted of 57.5– 87.5% dry rolled corn, 7.5% sorghum silage and 5% supplement. Increasing wet distillers grains in the diet had no effect on dry matter and organic matter intake but decreased dry matter and organic matter digestibility. Increasing CARS inclusion in the diet resulted in lower dry matter and organic matter intake …

Innovation In Meat Production: A Problem And An Opportunity, Christopher J. Bryant

Animal Sentience

Innovation in meat production has enabled modern humans to inflict far greater harm on animals, the environment, and public health than was possible just a few decades ago. Wiebers & Feigin aptly express the urgency with which these issues must be addressed. Those advocating for animals on moral grounds face resistance from omnivores citing taste, price and convenience. Further innovation in meat production (plant-based and cultured meat) will enable us to preserve the experience of eating meat whilst phasing out the many problems caused by industrial animal farming.

Prediction Of Warner-Bratzler Shear Force, Intramuscular Fat, Drip-Loss And Cook-Loss In Beef Via Raman Spectroscopy And Chemometrics, Raquel Cama-Moncunill, Jamie Cafferky, Caroline Augier, Torres Sweeney, Paul Allen, Alessandro Ferragina, Carl Sullivan, Andrew Cromie, Ruth Hamill

Articles

Rapid prediction of beef quality remains a challenge for meat processors. This study evaluated the potential of Raman spectroscopy followed by chemometrics for prediction of Warner-Bratzler shear force (WBSF), intramuscular fat (IMF), ultimate pH, drip-loss and cook-loss. PLS regression models were developed based on spectra recorded on frozen-thawed day 2 longissimus thoracis et lumborum muscle and validated using test sets randomly selected 3 times. With the exception of ultimate pH, models presented notable performance in calibration (R2 ranging from 0.5 to 0.9; low RMSEC) and, despite variability in the results, promising predictive ability: WBSF (RMSEP ranging from 4.6 to 9 …

Feeding The Future Meat Doesn’T Come Cheap, Lukas C. Southard

Capstones

Cultured – or as it is referred to by companies innovating the technology clean – meat is expected to be the next innovation to change the way the world gets its animal protein. Meat from animal cells grown in a lab seems like science fiction but it is around the corner from hitting your supermarket shelves. The technology has been developed but how these companies intend on scaling up their production to meet retail demands remains a mystery. So far companies have relied on seed and early stage investment from venture capital companies and private sources to fund research. Predictions …

Effects Of 50:50 Proportion Bull:Cow Blend Levels And Incorporation Of Finely Textured Beef On The Color Of Precooked Ground Beef Patties, Colton Allen Althaber

Graduate Theses and Dissertations

Variation in internal cooked color of ground beef is an economic concern for the ground beef market. Persistent pink color in hamburger patties can lead consumers to the perception of an undercooked product. Therefore, the objective of this study was to characterize the effects of bull:cow blend proportions, with or without finely textured beef (FTB), and cookery method on internal color of precooked ground beef patties. Batches (9.1 kg) of 85% lean ground beef were manufactured with 0, 33, 67, or 100% 50:50 bull:cow blend (remainder of lean was 100, 67, 33, or 0% Select-grade knuckles, respectively) mixed with 2 …

Methods To Evaluate Ruminant Animal Production Responses, Jose Manuel Diaz Gomez

Graduate Theses and Dissertations

In experiment 1, 80 steers (197.0 kg initial body weight; BW for fall, 116.9 kg for spring), were stocked at 2.45 and 4.1 calves/ha in fall and spring, respectively in 16 tall fescue pastures [fall ergovaline (EV) = 1,475 ppb and spring EV = 1,173 ppb] under 2 treatments, mineral (MIN) (n = 8) and cumulative management (CM) (n = 8). Forage allowance did not differ (P = 0.76) between CM and MIN during fall but differed during spring (P ≤ 0.05, 2.55 vs. 3.22 kg DM/kg BW, for MIN and CM, respectively). For fall, average daily gain (ADG) resulted …

Best Management Practices For Internal Parasite Control In Small Ruminants, Susan Schoenian, Enrique Nelson

Professional Agricultural Workers Journal

Abstract

Internal parasites represent a significant obstacle to a profitable and sustainable US goat industry. In some states, internal parasites are a year-round problem, while in others, they are a more seasonal challenge. Traditionally, parasite control methods relied heavily on antiparasitic drugs called anthelmintics or dewormers. Continued use and misuse of these drugs have resulted in increasingly resistant worm populations, necessitating more holistic or integrated methods of parasite control. A combination of practices will usually be necessary to achieve a satisfactory level of control. This paper discusses best management practices for controlling internal parasites in small ruminants, including pasture and …

Reproductive Technologies Used To Make Goats More Efficient, Seyedmehdi Mobini

Professional Agricultural Workers Journal

Abstract

With the introduction of Boer and Kiko breeds for meat goat production in the United States, more emphasis is being placed on the reproductive management to increase the number of offspring born and weaned and the frequency with which they are produced. It is also desirable to produce out of season kids to take advantage of a market premium for milk and meat. Reproductive manipulations, commercial Artificial Insemination (AI) programs using fresh or frozen semen, and Embryo Transfer (ET) have been developed and are in use most commonly in goats for reproductive efficiency.

Keywords: Meat Goats, Reproductive Technology, Reproductive …

Goat Mortality Composting, Roger Merkel

Professional Agricultural Workers Journal

One lawful method to dispose of animal carcasses is composting. Mortality composting is a “green” method that provides the producer with valuable soil amendments. Composting sites should be away from water sources and public areas. Some states require a facility and/or permit for carcass composting so producers should contact their State Veterinarian. Animal composting generates little to no odor and generates temperatures high enough to kill pathogens. Mortality composting is an aerobic process and requires a proper C:N ratio achieved by using a high carbon source to envelop the carcass, moisture content of roughly 50%, and available oxygen. Common composting …

Optimizing Reproductive Performance In The Goat Herd, Dahlia O'Brien, Stephen Wildeus

Professional Agricultural Workers Journal

Abstract

Reproductive efficiency impacts pregnancy rates, kidding/lambing frequency and number of offspring available for market each year. To enhance reproductive performance, consider breed, selection, nutrition and health, and understand basic female and male reproduction. Proper ration formulation and feeding promotes reproductive function and leads to more offspring. Selecting the highest performing females while culling unproductive does increases reproductive rates. Diseases affect the reproduction and survival of kids, so adopting disease prevention strategies including routine vaccinations and deworming protocols that promote good herd health is necessary. Understanding male and female reproduction are critical in managing puberty, minimizing the effects of seasonality …

Effects Of Poor Sanitation Procedures On Cross-Contamination Of Animal Species In Ground Meat Products, Sunjung Chung, Rosalee S. Hellberg

Food Science Faculty Articles and Research

The presence of <1% of an undeclared species in ground meat is generally thought to be indicative of cross-contamination as opposed to intentional mislabeling; however, this has not been experimentally tested. The objective of this study was to quantify the effects of poor sanitation on the cross-contamination of animal species in ground meat products, with the example of undeclared pork in ground beef. Cross-contamination was quantified using real-time polymerase chain reaction (PCR). Three different sanitation treatments were tested with a commercial grinder (“no cleaning”, “partial cleaning”, or “complete cleaning”) in between grinding of pork and beef samples (13.6 kg each). A 100-g sample was collected for each 0.91 kg (2 lb) of beef processed with the grinder and each sanitation treatment was tested twice. For the “no cleaning” treatment, the first 100-g sample of ground beef run through the grinder contained 24.42 ± 10.41% pork, while subsequent samples (n = 14) contained <0.2% pork. With “partial cleaning,” the first sample of ground beef contained 4.60 ± 0.3% pork and subsequent samples contained <0.2% pork. Pork was not detected in ground beef following “complete cleaning.” These results indicate that incomplete cleaning of grinding equipment leads to species cross-contamination at levels of <1% in most cases. Proper sanitation procedures must be followed when grinding multiple species in order to prevent cross-contamination and product mislabeling.

Development Of A Dna Mini-Barcoding Protocol Targeting Coi For The Identification Of Elasmobranch Species In Shark Cartilage Pills, Rowena J. Zahn, Anthony J. Silva, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Many elasmobranch (shark and ray) species are considered threatened and their identification in processed products is important for conservation and authentication purposes. However, identification of elasmobranch species in shark cartilage pills has proven difficult using existing methodologies. The objective of this study was to develop a DNA mini-barcoding protocol using a ~130 bp region of the cytochrome c oxidase subunit I (COI) gene for species identification in shark cartilage pills. A total of 22 shark cartilage products underwent DNA extraction in duplicate using the DNeasy Blood and Tissue Kit (Qiagen). The effectiveness of a clean-up step following DNA extraction was …