Meat Science Commons^™

Evaluating The Shelf Life And Sensory Properties Of Beef Steaks From Cattle Raised On Different Grass Feeding Systems In The Western United States, Toni L. Duarte, Bakytzhan Bolkenov, Sarah C. Klopatek, James W. Oltjen, D. Andy King, Steven D. Shackelford, Tommy L. Wheeler, Xiang Yang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Consumer interest in grass-fed beef has been steadily rising due to consumer perception of its potential benefits. This interest has led to a growing demand for niche market beef, particularly in the western United States. Therefore, the objective of this study was to assess the impact of feeding systems on the change in microbial counts, color, and lipid oxidation of steaks during retail display, and on their sensory attributes. The systems included: conventional grain-fed (CON), 20 months-grass-fed (20GF), 25-months-grass-fed (25GF) and 20-months-grass-fed + 45-day-grain-fed (45GR). The results indicate that steaks in the 20GF group displayed a darker lean and fat …

Native Beef Collagenase Mmp-9 May Contribute To Tenderness Improvement By Degrading Connective Tissues In Extended Aged Beef, L. A. Koulicoff, A. A. Welter, P. A. Hammond, C. K. Chun, T. G. O'Quinn, G. Magnin-Bissel, M. D. Chao

Kansas Agricultural Experiment Station Research Reports

Objective:Collagen is one of the main components in the connective tissue (CT) and contributes to background toughness in beef. It is known that in living animals, collagen can be degraded and remodeled by collagenase matrix metalloproteinases (MMP); however, it is unclear if collagenase MMP can impact CT texture during postmortem aging of beef. Therefore, this study aimed to understand how collagenase MMP activity may impact postmortem connective tissue degradation in beef in three different cuts and four different aging periods.

Study Description:Beef boneless striploin, top sirloin butt, and heel were acquired from 10 U.S. Department of Agriculture high …

An Investigation On The Influence Of Various Biochemical Tenderness Factors On Eight Different Bovine Muscles, P. A. Hammond, C. K. Chun, W. Wu, A. A. Welter, T. G. O'Quinn, G. Magnin-Bissel, E. Geisbrecht, M. D. Chao

Kansas Agricultural Experiment Station Research Reports

Objective:Beef tenderness is a complex palatability trait with many tenderness-contributing components. The objective of this study is to understand the relative contribution of each tenderness component to eight different beef muscles.

Study Description:Top sirloin butt, ribeye, brisket, flank, knuckle, eye of round, mock tender, and shoulder clod were collected from 10 U.S. Department of Agriculture high choice beef carcasses and assigned to a 2- or 21-day aging period (n = 160). Protein degradation, collagen content, mature collagen crosslink density, intramuscular lipid content, pH, shear force, and trained sensory panel analysis were determined. A Pearson correlation analysis was used …

Assessment Of Meat Quality Attributes Of Four Commercial Broiler Strains Processed At Various Market Weights, Ashleigh Jo Mueller

Graduate Theses and Dissertations

As the demand for poultry meat continues to grow, the industry is challenged with how to meet these needs of consumers. The growth rate of broilers is continuously improving in order to increase production efficiently resulting in greater yields and an increase in product to satisfy consumer needs. However, the industry has seen an increase in quality issues of the meat as growth rates and growth periods continue to increase. The objective of this study was to evaluate the effect of various strains (standard and high-yielding) and target weights on meat quality attributes such as pH, water holding capacity (WHC), …

A Preliminary Investigation Of The Contribution Of Different Tenderness Factors To Beef Loin, Tri-Tip, And Heel Tenderness, C. K. Chun, W. Wu, A. A. Welter, T. G. O'Quinn, G. Magnin-Bissel, D. L. Boyle, M. D. Chao

Kansas Agricultural Experiment Station Research Reports

Objective:The objective is to better understand the contribution of each tenderness factor to the perception of tenderness of three specific beef muscles with similar tenderness ratings.

Study Description:Longissimus lumborum (loin), tensor fascia latae (tri-tip), and gastrocnemius (heel) were collected from 10 U.S. Department of Agriculture low Choice beef carcasses and assigned to a 5- or 21-day aging period (n = 60). Steaks from each aging period from each subprimal were assigned to one of three assays: 1) trained sensory analysis; 2) objective tenderness evaluation (Warner-Bratzler shear force); or 3) physiochemical analysis (sarcomere length, proteolysis, intramuscular fat content, collagen …

Characterization Of Growth Patterns And Meat Quality Characteristics Of Four Commercial Broiler Strains In Small Bird And Large Bird Programs In The United States, Clay Johnny Maynard

Graduate Theses and Dissertations

Through current trends in animal protein consumption, the United States poultry industry has seen a drastic rise in production and popularity. Over the last few decades, poultry meat has surpassed both beef and pork production to become the most widely consumed animal protein. This rise may be accredited to an increased transition to more health-conscious consumers. As more consumers purchase poultry as a lean source of protein, the need for a superior quality product is of great interest to poultry integrators.

Relationships between commercial broiler lines have been well documented in previous years, but constant and intensive genetic selection in …

Free Calcium Concentration, Calpain-2 Activity, And Final Product Tenderness Of Electrically Stimulated Beef, B J. Buseman, T M. Weber, J A. Nasados, P D. Bass, J B. Van Buren, J M. Lancaster, J H. Smart, M E. Doumit, G K. Murdoch, W J. Price, K Insausti, M J. Colle

Department of Animal Science: Faculty Publications

The objective of this study was to evaluate the effect of timing of electrical stimulation on free calcium concentration, calpain-2 activity, Warner-Bratzler shear force (WBSF), and consumer sensory analysis. Twenty-three beef steers were harvested and stimulated (S) using extra-low voltage or not stimulated (NS), at exsanguination and/or 1 h postmortem, resulting in 4 different stimulation treatments: NS-NS, NS-S, S-NS, or S-S. Samples were removed from the longissimus lumborum (LL) and semimembranosus (SM) for free calcium and calpain-2 analysis on days 1, 4, and 14 postmortem. WBSF and sensory analysis steaks were removed on day 4 and frozen (4 d) or …

Using Genetic Panels To Predict Tenderness In Beef Cattle, Tanya M. Weber, Michael J. Colle, Gordon K. Murdoch, B J. Buseman, Jessica M. Lancaster, Jessie B. Van Buren, James A. Nasados, Phillip D. Bass

Department of Animal Science: Faculty Publications

Genetic panel use as a selection tool has grown in popularity in the beef industry. The objective of the study was to determine whether beef cattle genetically selected for tenderness generated a tender product. Igenity® (IT) panel results were provided by a cattle producer for 52 steers, which were harvested at a commercial harvest facility. Boneless strip loins (Institutional Meat Purchase Specifications #180; United States Department of Agriculture [USDA] Choice, n = 32; USDA Prime n = 20) were collected from the left side of each carcass and transported to the University of Idaho Meat Science Laboratory. Four steaks were …

Evaluation Of The Quality Characteristics Of Premium Pork Loins, L. L. Prill, L. N. Drey, E. A. Rice, T. G. O'Quinn

Kansas Agricultural Experiment Station Research Reports

The objective of this study was to determine shear force, pH, marbling, color characteristics, percentage of intramuscular fat, and purge loss of pork loins from various premium brands in comparison to commodity products. Pork loins (n = 30/brand; Institutional Meat Purchasing Specifications #414) from five premium (PRE A, B, C, D, and E) and two commodity brands (COM A and B) were purchased from food service purveyors and commercial abattoirs. Loins were transported to the Kansas State University Meat Laboratory, Manhattan, KS, and allowed to age 14 to 15 days under refrigerated conditions (36 to 39°F) before fabrication. All PRE …

Relationship Of Myofibrillar Fragmentation Index To Warner-Bratzler Shear Force And Palatability Tenderness Of Longissimus Lumborum And Semitendinosus Steaks, L. L. Prill, K. J. Phelps, J. M. Gonzalez, T. A. Houser, E. A. Boyle, T. G. O'Quinn

Kansas Agricultural Experiment Station Research Reports

Objective:The objective of this study was to determine the relationship between the myofibrillar fragmentation index, Warner-Bratzler shear force and sensory traits oflongissimus lumborum(strip loin), and thesemitendinosus(eye of round) steaks.

Study Description:Forty beef strip loins and 40 eye of rounds were collected, divided into anatomical location, and cut into steaks. Steaks used for Warner-Bratzler shear force and sensory panel review were cooked to 160°F. For Warner-Bratzler, six 1-in cores were sheared with a Warner-Bratzler shear head. Sensory steaks were served to panelists trained according to American Meat Science Association guidelines for sensory evaluation. Procedures described …

Brahman Genetics Negatively Impact Protein Degradation And Tenderness Of Longissimus Lumborum Steaks, But Do Not Influence Collagen Cross-Linking, K. J. Phelps, D. D. Johnson, M. A. Elzo, C. B. Paulk, J. M. Gonzalez

Kansas Agricultural Experiment Station Research Reports

Beef tenderness is an important factor contributing to consumer eating satisfaction of beef products. Tenderness is dependent on several factors including: breed-type, postmortem age time, myofibrillar muscle protein degradation, and collagen content. During the past 30 years, numerous studies have indicated steaks from cattle with a greater percentage of Brahman genetics are tougher than steaks from Bos taurus cattle. The cause of tougher steaks is commonly attributed to Brahman cattle having a greater calpastatin activity which inhibits calpains, the enzymes responsible for myofibrillar protein degradation during the postmortem aging process. Some researchers have reported calpastatin activity was poorly correlated to …

Age End-Point Effects On Performance, Carcass Measurements, And Tenderness In Goats, Kelsey Lynn Basinger

Graduate Theses and Dissertations

Goat meat is consumed all across the world, especially in developing countries. Moreover, the meat goat industry is growing in popularity in the United States due to ethnic demand of an increasing immigrant population. The objective of this study was to measure age end-point effects on performance, carcass measurements, and tenderness in goats. Intact Kiko × Boer F1 male (n = 46) kids were born on pasture in the spring of 2014 and 2015, weaned at approximately 100 d of age, and weighed monthly. Creep feed was offered to kids from birth to weaning and a high concentrate diet was …

Effects Of Postmortem Aging Time And Muscle Location On Objective Measures Of Semitendinosus Steak Tenderness, M. B. Da Silva, L. D. F. Miranda, K. J. Phelps, S. M. Ebarb, C. L. Van Bibber, T. G. O'Quinn, J. S. Drouillard, J. M. Gonzalez

Kansas Agricultural Experiment Station Research Reports

Beef tenderness is the most important trait affecting consumer beef eating satisfaction. Cuts from muscles with superior tenderness (tenderloin, ribeye, striploin) are consequently most highly valued, but because of recent record-high beef prices, the need to identify more economical cuts that will meet consumer expectations for tenderness is greater than ever. The eye of round (Semitendinosus) has traditionally been marketed as a lower-value cut, primarily owing to its inherent toughness. Tenderness improves throughout postmortem aging and continues to improve in muscles aged for greater than 35 days. Retail steaks from theSemitendinosusreceive, on average, 17 days of …

Growth Promoting Implants And Nutrient Restriction Prior To Feeding: Effect On Carcass Composition, Carcass Quality, And Consumer Acceptability Of Beef, Jessica Ann Robinette

Graduate Theses and Dissertations

Implant strategy and nutrient restriction prior to finishing may alter feedlot performance, as well as carcass characteristics and consumer acceptability of beef. The objectives of these studies were to determine the effect of prefinishing implant strategy and plane of nutrition on prefinishing and feedlot performance, carcass characteristics and quality, and consumer acceptability of beef. In 2 experiments, spring-born calves were weaned in the fall (Exp. 1, n =120; and Exp. 2, n = 96) and were either finished as calves (CALF-FED) or placed on a growing program with a target ADG of 0.45 kg/d (RSTR) or 0.91 kg/d (UNRSTR) before …

The Efficacy Of Three Objective Systems For Identifying Beef Cuts That Can Be Guaranteed Tender, T.L. Wheeler, D. Vote, J.M. Leheska, S.D. Shackelford, K.E. Belk, D.M. Wulf, B.L. Gwartney, M. Koohmaraie

Animal Science Faculty Publications

The objective of this study was to determine the accuracy of three objective systems (prototype BeefCam, colorimeter, and slice shear force) for identifying guaranteed tender beef. In Phase I, 308 carcasses (105 Top Choice, 101 Low Choice, and 102 Select) from two commercial plants were tested. In Phase II, 400 carcasses (200 rolled USDA Select and 200 rolled USDA Choice) from one commercial plant were tested. The three systems were evaluated based on progressive certification of the longissimus as “tender” in 10% increments (the best 10, 20, 30%, etc., certified as “tender” by each technology; 100% certification would mean no …

Mapping Intramuscular Tenderness Variation In Four Major Muscles Of The Beef Round, B.J. Reuter, D.M. Wulf, R.J. Maddock

Animal Science Faculty Publications

The objective of this study was to quantify intramuscular tenderness variation within four muscles from the beef round: biceps femoris (BF), semitendinosus (ST), semimembranosus (SM), and adductor (AD). At 48 h postmortem, the BF, ST, SM, and AD were dissected from either the left or right side of ten carcasses, vacuum packaged, and aged for an additional 8 d. Each muscle was then frozen and cut into 2.54- cm-thick steaks perpendicular to the long axis of the muscle. Steaks were broiled on electric broilers to an internal temperature of 71°C. Location-specific cores were obtained from each cooked steak, and Warner- …

Technical Note: The Effect Of Freezing On Warner-Bratzler Shear Force Values Of Beef Longissimus Steaks Across Several Postmortem Aging Periods, B.C. Shanks, D.M. Wulf, R.J. Maddock

Animal Science Faculty Publications

The objective of this study was to compare fresh and frozen protocol procedures for Warner- Bratzler shear force (WBSF) determination on steaks aged for different periods of time. The fresh protocol consisted of measuring WBSF on steaks cooked on the exact day the aging period ended. The frozen protocol consisted of measuring WBSF on steaks that were aged, frozen (−16°C) for approximately 2 mo, thawed for 24 h, and then cooked. Twenty-two strip loin steaks from each of 20 crossbred heifers and steers were individually vacuum-packaged and assigned to either the fresh or frozen protocol and one of 11 aging …

Relationships Among Glycolytic Potential, Dark Cutting (Dark, Firm, And Dry) Beef, And Cooked Beef Palatability, D.M. Wulf, R.S. Emnett, J.M. Leheska, S.J. Moeller

Animal Science Faculty Publications

One hundred beef carcasses were selected at three packing plants and were used to determine the relationship between glycolytic potential (GP) and dark, firm, and dry (DFD) beef and to determine the effects of DFD status and GP on cooked beef palatability. Eight individual muscles were excised from one hindquarter of each carcass at d 7 postmortem: longissimus lumborum, psoas major, gluteus medius, tensor fasciae latae, rectus femoris, semimembranosus, biceps femoris, and semitendinosus. Ultimate pH, colorimeter readings, and Warner-Bratzler shear force were determined for all eight muscles at d 7 postmortem. A ninemember trained sensory panel evaluated cooked longissimus lumborum, …

Evaluating The Point Of Separation, During Carcass Fabrication, Between The Beef Wholesale Rib And The Beef Wholesale Chuck, B.J. Reuter, D.M. Wulf, B.C. Shanks, R.J. Maddock

Animal Science Faculty Publications

This study determined whether there is a logical point of value change, related to either tenderness or consumer acceptance, at which to separate the beef carcass within the rib/chuck region. Rib/chuck rolls (RCR); (n = 30) consisting of the ribeye roll and chuck eye roll subprimals (2nd through 12th rib locations) were cut into 22 steaks each (two steaks per rib location), and Warner-Bratzler shear force and consumer purchase preference were evaluated for steaks at each rib location. Steaks from different locations of the RCR were composed of differing proportions of several muscles: longissimus muscle (LM), spinalis dorsi and multifidus …

A Muscle Hypertrophy Condition In Lamb (Callipyge): Characterization Of Effects On Muscle Growth And Meat Quality Traits, M. Koohmaraie, S. D. Shackelford, T. L. Wheeler, Steven M. Lonergan, M. E. Doumit

Steven M. Lonergan

The present experiment was conducted to determine the effect of the callipyge phenotype on traits affecting muscle growth and meat tenderness. Dorset wethers (n = 40) that were either carriers or non-carriers were fed grain and slaughtered at 169 d of age. Callipyge phenotype did not affect ( P > .05) slaughter weight, hot carcass weight, or weights of the heart, spleen, viscera, kidney-pelvic fat, head, and pelt; however, callipyge lambs had a higher dressing percentage and lighter lungs, liver, and kidneys ( P < .01). Callipyge lambs had reduced fat thickness and marbling score and higher leg scores and longissimus area (34%). Adductor (30%), biceps femoris (42%), gluteus group (31%), longissimus (32%), psoas group (20%), quadriceps femoris (18%), semimembranosus (38%), and semitendinosus (26%) weights were higher in the callipyge phenotype ( P < .01); however, phenotype did not affect ( P > .05) weights of infraspinatus or supraspinatus. Longissimus pH and temperature declines were not affected ( P > .05) …

Relationship Of Restriction Fragment Length Polymorphisms (Rflp) At The Bovine Calpastatin Locus To Calpastatin Activity And Meat Tenderness, Steven M. Lonergan, C. W. Ernst, M. D. Bishop, C. R. Calkins, M. Koohmaraie

Steven M. Lonergan

Restriction fragment length polymorphisms (RFLP) have been identified at the bovine calpastatin locus. The objective of the present study was to determine whether these polymorphisms are related to variations in calpastatin activity or beef tenderness in unrelated animals of mixed breeding. A sample of 83 crossbred steers from sires representing eight different breeds was examined to determine this relationship. A 2.2-kb cDNA coding for domains 2 through 4 plus a 3¢ untranslated region of bovine skeletal muscle calpastatin was used as a probe for calpastatin RFLP. Polymorphisms were found using the restriction enzymes BamHI and EcoRI. Polymorphic restriction fragments for …