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Full-Text Articles in Meat Science

One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika Jun 2025

One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika

All Peer-Reviewed Publications

The global human population is rapidly increasing, escalating interactions of people, animals and the environment. This has led to more convoluted health challenges, for which African smallholder farmers bear the brunt. The One Health (OH) approach recognises the interconnectedness of these health challenges and thus follows a transdisciplinary approach involving diverse stakeholders to address them. Although there have recently been global concerted efforts and studies on OH, an information gap exists in Africa, particularly regarding smallholder farmers and their needs. Additionally, evaluation frameworks and outcome metrics for OH initiatives are still unclear. Thus, this study was conducted to critically map …


Confined Paddock Feeding And Feedlotting Of Sheep, Department Of Primary Industries And Regional Development, Western Australia Nov 2024

Confined Paddock Feeding And Feedlotting Of Sheep, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Confinement feeding (also referred to as lot feeding or feedlotting) is an intensive feeding system in a confined area where all, or the majority of, feed and water is supplied to the contained animals. The department recommends using confinement feeding as part of a whole farm livestock, pasture and erosion management program.

We recommend having a confinement feeding system as an integral part of a whole farm livestock, pasture and erosion management program. Guides and resources that provide more detailed information are listed at the bottom of this fact sheet.


Breeding Ewes Are Worth Feeding, Department Of Primary Industries And Regional Development, Western Australia Sep 2024

Breeding Ewes Are Worth Feeding, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Despite potentially elevated grain prices, strong prices for meat and wool mean that Western Australian sheep farmers can confidently supplementary feed their underweight ewes in summer/autumn.

Analysis in 2019, based on then-current prices, showed it was a profitable investment to feed all ewes to maintain condition during pregnancy, and to feed low condition score single bearing ewes and low and medium condition score twin bearing ewes to gain condition.

In 2019, John Young from Farming Systems Analysis Service completed an analysis on the value of a ewe in the WA farming system. His report had some important messages for the …


Alternative Energy And Protein Feed Sources For Sheep And Cattle, Department Of Primary Industries And Regional Development, Western Australia May 2024

Alternative Energy And Protein Feed Sources For Sheep And Cattle, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

During dry times and drought there are a wide range of alternative feedstuffs producers may consider using to maintain stock. Some of these feeds can have health risks associated with them and should not be used as the sole food source as they often lack sufficient energy and protein.


Greenfeed Methane Measuring Machine, Department Of Primary Industries And Regional Development, Western Australia Jan 2024

Greenfeed Methane Measuring Machine, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

The Department of Primary Industries and Regional Development (DPIRD) now has the cutting-edge GreenFeed machine which measures emissions from individual cows and sheep, allowing us to determine Greenhouse Gas (GHG) emissions more accurately in the paddock.


Carbon Neutral By 2030 - Katanning Research Station, Department Of Primary Industries And Regional Development, Western Australia Jan 2024

Carbon Neutral By 2030 - Katanning Research Station, Department Of Primary Industries And Regional Development, Western Australia

Climate Science Published Reports

The Department of Primary Industries and Regional Development's (DPIRD) Katanning Research Station (KRS) is developing practical techniques and methods for mitigating carbon emissions from the livestock industry.

This research will help the livestock industry in Western Australia (WA) access anticipated future markets for carbon neutral food and fibre products.


Mirna Regulation Of Skeletal Muscle Hypertrophy, Maslyn Greene Aug 2023

Mirna Regulation Of Skeletal Muscle Hypertrophy, Maslyn Greene

All Dissertations

Muscle tissue is vital to animal survival and economically essential to livestock producers. Reduced muscle growth can occur from various reasons, including genetics, nutrition, and the pre and postnatal environment. Understanding the mechanisms behind muscle growth can aid in developing strategies to increase muscle mass. Several methods to increase muscle mass in livestock animals are currently being pursued, including nutrition, hormonal therapy, and genetic selection. One newly developing avenue is using miRNA technologies to change the expression of protein-coding genes. miRNA are a type of RNA that regulate over half of protein-coding gene expression. Here we evaluate muscle characteristics such …


Microbial Community Structure From Southern High Plains Beef Cattle Feedyard Manure And Relationship With Nitrous Oxide Emissions, Heidi Waldrip, David Parker, Sierra Miller, Lisa M. Durso, Byeng R. Min, Daniel N. Miller, Kenneth Casey, Bryan Woodbury, Mindy J. Spiehs Oct 2022

Microbial Community Structure From Southern High Plains Beef Cattle Feedyard Manure And Relationship With Nitrous Oxide Emissions, Heidi Waldrip, David Parker, Sierra Miller, Lisa M. Durso, Byeng R. Min, Daniel N. Miller, Kenneth Casey, Bryan Woodbury, Mindy J. Spiehs

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Modern molecular techniques enable characterization of the microbial biome in livestock manure, from which there is particular concern over emission of greenhouse gases. This study evaluated how sampling depth, time, temperature, and artificial rainfall affected microbial community structure in feedyard manure, and relationships between the manure biome and known parameters related to nitrous oxide (N2O) emissions. In three large incubation chambers, maintained at different temperatures that received two applications of artificial rainfall, we evaluated manure microbiome composition and abundance of N2O-producing enzymes (nirK and nirS) using quantitative polymerase chain reaction (qPCR). These data were …


Evaluating The Shelf Life And Sensory Properties Of Beef Steaks From Cattle Raised On Different Grass Feeding Systems In The Western United States, Toni L. Duarte, Bakytzhan Bolkenov, Sarah C. Klopatek, James W. Oltjen, D. Andy King, Steven D. Shackelford, Tommy L. Wheeler, Xiang Yang Jul 2022

Evaluating The Shelf Life And Sensory Properties Of Beef Steaks From Cattle Raised On Different Grass Feeding Systems In The Western United States, Toni L. Duarte, Bakytzhan Bolkenov, Sarah C. Klopatek, James W. Oltjen, D. Andy King, Steven D. Shackelford, Tommy L. Wheeler, Xiang Yang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Consumer interest in grass-fed beef has been steadily rising due to consumer perception of its potential benefits. This interest has led to a growing demand for niche market beef, particularly in the western United States. Therefore, the objective of this study was to assess the impact of feeding systems on the change in microbial counts, color, and lipid oxidation of steaks during retail display, and on their sensory attributes. The systems included: conventional grain-fed (CON), 20 months-grass-fed (20GF), 25-months-grass-fed (25GF) and 20-months-grass-fed + 45-day-grain-fed (45GR). The results indicate that steaks in the 20GF group displayed a darker lean and fat …


Genes Involved In Feed Efficiency Identified In A Meta-Analysis Of Rumen Tissue From Two Populations Of Beef Steers, Amanda K. Lindholm-Perry, Allison M. Meyer, Rebecca J. Kern-Lunbery, Hannah C. Cunningham-Hollinger, Taran H. Funk, Brittney N. Keel Jun 2022

Genes Involved In Feed Efficiency Identified In A Meta-Analysis Of Rumen Tissue From Two Populations Of Beef Steers, Amanda K. Lindholm-Perry, Allison M. Meyer, Rebecca J. Kern-Lunbery, Hannah C. Cunningham-Hollinger, Taran H. Funk, Brittney N. Keel

Roman L. Hruska U.S. Meat Animal Research Center: Reports

In cattle, the rumen is an important site for the absorption of feed by-products released by bacterial fermentation, and variation in ruminal function plays a role in cattle feed efficiency. Studies evaluating gene expression in the rumen tissue have been performed prior to this. However, validating the expression of genes identified in additional cattle populations has been challenging. The purpose of this study was to perform a meta-analysis of the ruminal transcriptome of two unrelated populations of animals to identify genes that are involved in feed efficiency across populations. RNAseq data from animals with high and low residual feed intake …


Prenatal Transportation Stress Did Not Impact Ovarian Follicle Count For Three Generations Of Female Brahman Offspring, Lacey K. Quail, Ronald D. Randel, Thomas H. Welsh Jr., Robert A. Cushman, Hannah K. Yake, Rui A. D'Orey Branco, Donald A. Neuendorff, Charles R. Long, George A. Perry Jun 2022

Prenatal Transportation Stress Did Not Impact Ovarian Follicle Count For Three Generations Of Female Brahman Offspring, Lacey K. Quail, Ronald D. Randel, Thomas H. Welsh Jr., Robert A. Cushman, Hannah K. Yake, Rui A. D'Orey Branco, Donald A. Neuendorff, Charles R. Long, George A. Perry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

As prenatal transportation stress altered behavior and adrenal glucocorticoid secretion of calves, we hypothesized that prenatal transportation stress would decrease ovarian reserve size and negatively impact female offspring fertility. The impact of prenatal transportation stress on ovarian follicle numbers in female offspring for three generations was studied. Brahman cows were transported for 2 h on day 60 ± 5, 80 ± 5, 100 ± 5, 120 ± 5, and 140 ± 5 of gestation. Ovaries were collected from offspring of transported or non-transported dams at multiple ages. Primordial, primary, secondary, and antral follicles were histologically analyzed. Antral follicle numbers were …


Efficacy Of Antimicrobial Interventions Used In Meat Processing Plants Against Antimicrobial Tolerant Non–Antibiotic-Resistant And Antibiotic-Resistant Salmonella On Fresh Beef, Norasak Kalchayanand, Sapna Chitlapilly Dass, Yangjunna Zhang, Eric L. Oliver, Bing Wang, Tommy L. Wheeler Jun 2022

Efficacy Of Antimicrobial Interventions Used In Meat Processing Plants Against Antimicrobial Tolerant Non–Antibiotic-Resistant And Antibiotic-Resistant Salmonella On Fresh Beef, Norasak Kalchayanand, Sapna Chitlapilly Dass, Yangjunna Zhang, Eric L. Oliver, Bing Wang, Tommy L. Wheeler

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Salmonella is a common cause of foodborne illness in the United States, and several strains of Salmonella have been identified as resistant to antibiotics. It is not known whether strains that are antibiotic resistant (ABR) and that have some tolerance to antimicrobial compounds are also able to resist the inactivation effects of antimicrobial interventions used in fresh meat processing. Sixty-eight Salmonella isolates (non-ABR and ABR strains) were treated with half concentrations of lactic acid (LA), peracetic acid (PAA), and cetylpyridinium chloride (CPC), which are used in beef processing plants to screen for tolerant strains. Six strains each from non-ABR and …


Effects Of Administration Of A Growth Promoting Implant During The Suckling Phase Or At Weaning On Growth, Reproduction, And Ovarian Development In Replacement Heifers Grazing Native Range, Shelby L. Rosasco, Emily A. Melchior-Tiffany, Cierrah J. Kassetas, Shad H. Cox, Richard L. Dunlap, Jennifer A. Hernandez Gifford, Eric J. Scholljegerdes, Robert A. Cushman, Adam F. Summers May 2022

Effects Of Administration Of A Growth Promoting Implant During The Suckling Phase Or At Weaning On Growth, Reproduction, And Ovarian Development In Replacement Heifers Grazing Native Range, Shelby L. Rosasco, Emily A. Melchior-Tiffany, Cierrah J. Kassetas, Shad H. Cox, Richard L. Dunlap, Jennifer A. Hernandez Gifford, Eric J. Scholljegerdes, Robert A. Cushman, Adam F. Summers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Management strategies utilized during pre-breeding development of replacement heifers can impact fertility and the ovarian reserve. Angus- Hereford crossbred heifers (n = 233) were utilized over a 3-yr period to determine the effects of administration of a growth promoting implant at either branding or weaning on growth, reproduction, and ovarian development. Heifer calves were randomly assigned to one of three treatments: 1) nonimplanted controls (CON; n = 79), 2) implanted at approximately 2 mo of age (average calf age = 58 d) with Synovex-C (BIMP, n = 82), or 3) implanted at approximately 7 mo of age (average calf …


Interaction Of Replacing Corn Silage With Soyhulls As A Roughage Source With Or Without 3% Added Wheat Straw In The Diet: Impacts On Intake, Digestibility, And Ruminal Fermentation In Steers Fed High-Concentrate Diets, Bryan W. Neville, Wayde J. Pickinpaugh, Lea J. Mittleider, Rebecca L. Moore, Kendall C. Swanson, Joel S. Caton May 2022

Interaction Of Replacing Corn Silage With Soyhulls As A Roughage Source With Or Without 3% Added Wheat Straw In The Diet: Impacts On Intake, Digestibility, And Ruminal Fermentation In Steers Fed High-Concentrate Diets, Bryan W. Neville, Wayde J. Pickinpaugh, Lea J. Mittleider, Rebecca L. Moore, Kendall C. Swanson, Joel S. Caton

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Six ruminally cannulated steers [475.0 ± 49.6 kg initial body weight (BW)] were used in a 6 × 3 incomplete Latin square design (six treatments and three periods), to evaluate the impacts replacing of corn silage with pelleted soyhulls as roughage in high-concentrate finishing diets containing 30% modified distillers grains with solubles. Treatments were based on increasing dietary inclusion of soyhulls and consisted of: (1) Control (0), roughage supplied by dietary inclusion of 20% corn silage [dry matter (DM) basis]; (2) 50% replacement of corn silage with soyhulls (50); (3) 100% replacement of corn silage with soyhulls (100), and the …


Development And Validation Of High-Resolution Melting Assays For The Detection Of Potentially Virulent Strains Of Escherichia Coli O103 And O121, Frank J. Velez, Joseph M. Bosilevac, Sabine Delannoy, Patrick Fach, Ravinder Nagpal, Prashant Singh May 2022

Development And Validation Of High-Resolution Melting Assays For The Detection Of Potentially Virulent Strains Of Escherichia Coli O103 And O121, Frank J. Velez, Joseph M. Bosilevac, Sabine Delannoy, Patrick Fach, Ravinder Nagpal, Prashant Singh

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Virulent strains of Shiga toxin-producing Escherichia coli (STEC) serogroups O103 and O121 are considered adulterants in beef. Two high-resolution melting (HRM) real-time PCR assays were standardized for the specific detection and discrimination of potentially virulent and avirulent strains of E. coli O103 and O121. The O103 HRM assay offered the possibility to distinguish clearly STEC O103:H2 from STEC O103:H25. The two standardized assays were extensively validated using 215 pure culture strains, laboratory inoculated food samples, and naturally contaminated beef (n = 84) and pork (n = 84) enrichments collected from the red meat surveillance program. Both HRM assays showed 100% …


Impacts Of Added Roughage On Growth Performance, Digestibility, Ruminal Fermentation, And Ruminal Ph Of Feedlot Steers Fed Wheat-Based Feedlot Diets Containing 30% Modified Distillers Grains With Solubles, Wayde J. Pickinpaugh, Bryan W. Neville, Rebecca L. Moore, Joel S. Caton May 2022

Impacts Of Added Roughage On Growth Performance, Digestibility, Ruminal Fermentation, And Ruminal Ph Of Feedlot Steers Fed Wheat-Based Feedlot Diets Containing 30% Modified Distillers Grains With Solubles, Wayde J. Pickinpaugh, Bryan W. Neville, Rebecca L. Moore, Joel S. Caton

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Two experiments were conducted to evaluate the inclusion rate roughage in wheat-based diets containing modified distillers grains with solubles (MDGS) on feedlot performance (Feedlot Experiment), as well as digestibility, ruminal pH, and ruminal fermentation characteristics (Digestibility Experiment). The feedlot experiment utilized 72 Angus steers (392 ± 46.3 kg initial body weight) which were randomly assigned to 1 of 12 pens, 3 pens per treatment, to evaluate feedlot performance and carcass characteristics. Dietary treatments were 1) control; 10% roughage, 2) 12% roughage, 3) 14% roughage, and 4) 16% roughage. The digestibility experiment used four ruminally and duodenally cannulated steers (393 ± …


Greater Numbers Of Antral Follicles In The Ovary Are Associated With Increased Concentrations Of Glucose In Uterine Luminal Fluid Of Beef Heifers, Alexandria P. Snider, Matthew S. Crouse, Shelby L. Rosasco, Kaitlin M. Epperson, Emmalee J. Northrop-Albrecht, Jerica J.J. Rich, Chadwick C. Chase Jr., Jeremy R. Miles, George A. Perry, Adam F. Summers, Robert A. Cushman Mar 2022

Greater Numbers Of Antral Follicles In The Ovary Are Associated With Increased Concentrations Of Glucose In Uterine Luminal Fluid Of Beef Heifers, Alexandria P. Snider, Matthew S. Crouse, Shelby L. Rosasco, Kaitlin M. Epperson, Emmalee J. Northrop-Albrecht, Jerica J.J. Rich, Chadwick C. Chase Jr., Jeremy R. Miles, George A. Perry, Adam F. Summers, Robert A. Cushman

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Increased antral follicles are associated with greater fertility and a uterine environment that is more supportive of early embryonic development in beef heifers. Glucose is a primary energy source for embryos, and glucose concentrations are elevated in uterine luminal fluid (ULF) of pregnant heifers. We hypothesized that ULF glucose concentrations and endometrial transcript abundance for glucose transporters on d16 after insemination would be greater in heifers with increased numbers of antral follicles. Heifers classified with either increased or diminished antral follicle counts were artificially inseminated following the CO-Synch protocol (d0). On d16 after insemination, reproductive tracts of heifers were collected …


Proteomic Analyses Identify Differences Between Bovine Epididymal And Ejaculated Spermatozoa That Contribute To Longevity, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie A. Walker, Robert A. Cushman, George A. Perry Mar 2022

Proteomic Analyses Identify Differences Between Bovine Epididymal And Ejaculated Spermatozoa That Contribute To Longevity, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie A. Walker, Robert A. Cushman, George A. Perry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Sperm are stored for extended periods of time in the epididymis, but upon ejaculation motility is increased and lifespan is decreased. The objective of this study was to identify differences in proteins between epididymis and ejaculated samples that are associated with longevity. Ejaculated semen was collected from mature Angus bulls (n = 9); bulls were slaughtered and epididymal semen was collected. Epididymal and ejaculated semen were centrifuged to separate sperm and fluid. Fluids were removed and sperm pellets were resuspended in a high ionic solution and vortexed to remove loosely attached proteins. Sperm samples were centrifuged, and the supernatant was …


Analysis Of Livestock Movements And The Benefits And Costs Of Livestock Standstills In Australia As Part Of The Response To Foot-And-Mouth Disease, Ann Seitzinger, Graeme Garner, Richard Bradhurst, Walter Okelo, Michelle Rodan Mar 2022

Analysis Of Livestock Movements And The Benefits And Costs Of Livestock Standstills In Australia As Part Of The Response To Foot-And-Mouth Disease, Ann Seitzinger, Graeme Garner, Richard Bradhurst, Walter Okelo, Michelle Rodan

Animal production and livestock published reports

This project involved analysis of livestock movements and used epidemiological modelling and cost analysis to estimate the benefits and costs of implementing livestock standstills in response to a foot-and-mouth disease (FMD) outbreak in Australia. This was done in four steps: 1) assessing the extent of livestock movement, 2) conducting a cross sectional analysis of the impact (reduction of outbreak size and economic cost) of implementing a national standstill/movement control, 3) use of case specific studies to estimate impact of state/territory level implementation of standstill, and 4) cost analysis and comparison of national standstill with the state/jurisdictional level and risk-based approach …


Effects Of Increasing Dietary Zinc Sulfate Fed To Gestating Ewes: Ii. Milk Somatic Cell Count, Microbial Populations, And Fatty Acid Composition, C. M. Page, R. M. Knuth, T. W. Murphy, D. C. Rule, B. Bisha, J. B. Taylor, W. C. Stewart Jan 2022

Effects Of Increasing Dietary Zinc Sulfate Fed To Gestating Ewes: Ii. Milk Somatic Cell Count, Microbial Populations, And Fatty Acid Composition, C. M. Page, R. M. Knuth, T. W. Murphy, D. C. Rule, B. Bisha, J. B. Taylor, W. C. Stewart

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Objective: The objective of the research was to evaluate the effects of increasing dietary Zn sulfate concentration for primiparous gestating ewes on subsequent milk SCC, intramammary microbial identifications, and fatty acid composition.

Materials and Methods: Commercial white-face (WF; n = 27) and black-face (BF; n = 24) ewes (age ≈18 mo; BW = 87.48 ± 8.37 kg) were sorted into breed-type groups and within groups ranked by BW, and then, they were randomly divided into 3 dietary supplement treatment groups: CON (n = 13; 40 mg/kg Zn; ≈1 × NASEM recommendations), Zn500 (n = 21; 500 mg/kg Zn; …


Effects Of Harvest Season On Carcass Characteristics Of Lambs In The Intermountain West, J. R. Whaley, T. W. Murphy, C. L. Gifford, W. J. Means, J. P. Ritten, H. N. Mckibben, C. M. Page, W. C. Stewart Jan 2022

Effects Of Harvest Season On Carcass Characteristics Of Lambs In The Intermountain West, J. R. Whaley, T. W. Murphy, C. L. Gifford, W. J. Means, J. P. Ritten, H. N. Mckibben, C. M. Page, W. C. Stewart

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Objective: The objectives of this study were to survey characteristics including hot carcass weight (HCW), 12th rib fat thickness (RFT), body-wall thickness (BWT), longissimus muscle area (LMA), USDA yield grade (USDA YG), percentage closely trimmed retail cuts (RC), and calculated yield grade (Calc YG) of lamb carcasses in the Intermountain West to determine the effects of season of slaughter and interrelationships among carcass characteristics.

Materials and Methods: Lamb carcass characteristics were evaluated in 2 commercial Intermountain West processing plants over one year (n = 10,027). Carcasses were evaluated by season: spring (December–April, n = 2,322) and summer (May–August, n …


Evaluation Of Salmonella Biofilm Cell Transfer From Common Food Contact Surfaces To Beef Products, Rong Wong, David A. King, Norasak Kalchayanand Dec 2021

Evaluation Of Salmonella Biofilm Cell Transfer From Common Food Contact Surfaces To Beef Products, Rong Wong, David A. King, Norasak Kalchayanand

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Meat contamination by Salmonella enterica is a serious public health concern. Available data have suggested that biofilm formation at processing plants and contaminated contact surfaces might contribute to meat contamination. Because transfer from contact surfaces to food products via direct contact has been deemed the most common bacteria transmission route that can lead to contamination, we evaluated the effect of Salmonella biofilm forming ability, contact surface material, and beef surface tissue type on Salmonella biofilm transfer from hard surfaces to beef products. Salmonella biofilms developed on the common contact surfaces stainless steel (SS) and polyvinylchloride (PVC) were transferred consecutively via …


Considerations And Best Practices In Animal Science 16s Ribosomal Rna Gene Sequencing Microbiome Studies, Margaret D. Weinroth, Aeriel D. Belk, Chris Dean, Noelle Noyes, Dana K. Dittoe, Michael J. Rothrock Jr, Steven C. Ricke, Phillip R. Myer, Madison T. Henniger, Gustavo A. Ramírez, Brian B. Oakley, Katie Lynn Summers, Asha M. Miles, Taylor B. Ault-Seay, Zhongtang Yu, Jessica L. Metcalf, James E. Wells Nov 2021

Considerations And Best Practices In Animal Science 16s Ribosomal Rna Gene Sequencing Microbiome Studies, Margaret D. Weinroth, Aeriel D. Belk, Chris Dean, Noelle Noyes, Dana K. Dittoe, Michael J. Rothrock Jr, Steven C. Ricke, Phillip R. Myer, Madison T. Henniger, Gustavo A. Ramírez, Brian B. Oakley, Katie Lynn Summers, Asha M. Miles, Taylor B. Ault-Seay, Zhongtang Yu, Jessica L. Metcalf, James E. Wells

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Microbiome studies in animal science using 16S rRNA gene sequencing have become increasingly common in recent years as sequencing costs continue to fall and bioinformatic tools become more powerful and user-friendly. The combination of molecular biology, microbiology, microbial ecology, computer science, and bioinformatics—in addition to the traditional considerations when conducting an animal science study—makes microbiome studies sometimes intimidating due to the intersection of different fields. The objective of this review is to serve as a jumping-off point for those animal scientists less familiar with 16S rRNA gene sequencing and analyses and to bring up common issues and concerns that arise …


Twenty-Four-Month Longitudinal Study Suggests Little To No Horizontal Gene Transfer In Situ Between Third-Generation Cephalosporin-Resistant Salmonella And Third-Generation Cephalosporin-Resistant Escherichia Coli In A Beef Cattle Feedyard, John W. Schmidt, Sarah A. Murray, Aaron M. Dickey, Tommy L. Wheeler, Dayna M. Harhay, Terrance M. Arthur Nov 2021

Twenty-Four-Month Longitudinal Study Suggests Little To No Horizontal Gene Transfer In Situ Between Third-Generation Cephalosporin-Resistant Salmonella And Third-Generation Cephalosporin-Resistant Escherichia Coli In A Beef Cattle Feedyard, John W. Schmidt, Sarah A. Murray, Aaron M. Dickey, Tommy L. Wheeler, Dayna M. Harhay, Terrance M. Arthur

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Third-generation cephalosporins (3GCs) are preferred treatments for serious human Salmonella enterica infections. Beef cattle are suspected to contribute to human 3GC-resistant Salmonella infections. Commensal 3GC-resistant Escherichia coli are thought to act as reservoirs of 3GC resistance because these strains are isolated more frequently than are 3GC-resistant Salmonella strains at beef cattle feedyards. During each of 24 consecutive months, four samples of pen surface material were obtained from five pens (N = 480) at a Nebraska feedyard to determine to the contribution of 3GC-resistant E. coli to the occurrence of 3GC-resistant Salmonella. Illumina whole genome sequencing was performed, and …


Meat Tenderness: Advances In Biology, Biochemistry, Molecular Mechanisms And New Technologies, Robyn D. Warner, Tommy L. Wheeler, Minh Ha, Xin Li, Alaa El-Din Bekhit, James Morton, Rozita Vaskoska, Frank R. Dunshea, Rui Lui, Peter Purslow, Wangang Zhang Aug 2021

Meat Tenderness: Advances In Biology, Biochemistry, Molecular Mechanisms And New Technologies, Robyn D. Warner, Tommy L. Wheeler, Minh Ha, Xin Li, Alaa El-Din Bekhit, James Morton, Rozita Vaskoska, Frank R. Dunshea, Rui Lui, Peter Purslow, Wangang Zhang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Meat tenderness is an important quality trait critical to consumer acceptance, and determines satisfaction, repeat purchase and willingness-to-pay premium prices. Recent advances in tenderness research from a variety of perspectives are presented. Our understanding of molecular factors influencing tenderization are discussed in relation to glycolysis, calcium release, protease activation, apoptosis and heat shock proteins, the use of proteomic analysis for monitoring changes, proteomic biomarkers and oxidative/nitrosative stress. Each of these structural, metabolic and molecular determinants of meat tenderness are then discussed in greater detail in relation to animal variation, postmortem influences, and changes during cooking, with a focus on recent …


Gain-Of-Function Polymorphisms In Human Inflammasomes: Implications For Cystic Fibrosis, Duane Jeansonne, Samithamby Jeyaseelan Aug 2021

Gain-Of-Function Polymorphisms In Human Inflammasomes: Implications For Cystic Fibrosis, Duane Jeansonne, Samithamby Jeyaseelan

Faculty Publications

No abstract provided.


Sustainable Winter-Feeding Practices For Meat Goats Within The Southeastern United States, Nikisha Ryan, Nar Gurung, Jeremy Byrant, Frank Abrahamsen, Wendell Mcelhenney Apr 2021

Sustainable Winter-Feeding Practices For Meat Goats Within The Southeastern United States, Nikisha Ryan, Nar Gurung, Jeremy Byrant, Frank Abrahamsen, Wendell Mcelhenney

Professional Agricultural Workers Journal

Winter feeding systems were evaluated on male Kiko meat goats against the traditional practice of raising goats in the winter months. Forty-Five Kiko bucks were randomly assigned to one of three treatments; Treatment 1, Treatment 2, and Treatment 3 consisted, respectively, of Cosaque Oats, annual Ryegrass, Crimson Clover mix, and 1.0% of BW concentrate supplementation with free choice hay. Forage clippings were analyzed for dry matter, crude protein, and neutral detergent fiber. Body weights were monitored over the grazing period. Blood samples were collected during the experiment, and animals were slaughtered for the evaluation of carcass traits. Data were analyzed …


Impact Of Mixed Biofilm Formation With Environmental Microorganisms On E. Coli O157:H7 Survival Against Sanitization, Sapna Chitlapilly Dass, Joseph M. Bosilevac, Maggie Weinroth, Christian Elowsky, You Zhou, Angela Anandappa, Rong Wang Oct 2020

Impact Of Mixed Biofilm Formation With Environmental Microorganisms On E. Coli O157:H7 Survival Against Sanitization, Sapna Chitlapilly Dass, Joseph M. Bosilevac, Maggie Weinroth, Christian Elowsky, You Zhou, Angela Anandappa, Rong Wang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Biofilm formation by foodborne pathogens is a serious threat to food safety and public health. Meat processing plants may harbor various microorganisms and occasional foodborne pathogens; thus, the environmental microbial community might impact pathogen survival via mixed biofilm formation. We collected floor drain samples from two beef plants with different E. coli O157:H7 prevalence history and investigated the effects of the environmental microorganisms on pathogen sanitizer tolerance. The results showed that biofilm forming ability and bacterial species composition varied considerably based on the plants and drain locations. E. coli O157:H7 cells obtained significantly higher sanitizer tolerance in mixed biofilms by …


Effect Of Method Of Drying Piglets At Birth On Rectal Temperature Over The First 24 H After Birth, Katherine D. Vande Pol, Andres F. Tolosa, Caleb M. Shull, Catherine B. Brown, Stephan A.S. Alencar, Michael Ellis Oct 2020

Effect Of Method Of Drying Piglets At Birth On Rectal Temperature Over The First 24 H After Birth, Katherine D. Vande Pol, Andres F. Tolosa, Caleb M. Shull, Catherine B. Brown, Stephan A.S. Alencar, Michael Ellis

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Piglets are born wet, and evaporation of that moisture decreases body temperature, increasing the risk of mortality. The objective of this study was to compare the effect of two commercially applicable methods for drying piglets at birth on piglet rectal temperature over 24 h after birth. The study was carried out in standard commercial farrowing facilities with 52 litters, using a completely randomized design with three Drying Treatments: Control (not dried); Desiccant (dried at birth using a cellulose-based desiccant); Paper Towel (dried at birth using paper towels). Litters were randomly allotted to treatments at the birth of the first piglet. …


Effect Of Drying And/Or Warming Piglets At Birth On Rectal Temperature Over The First 24 H After Birth, Katherine D. Vande Pol, Andres F. Tolosa, Caleb M. Shull, Catherine B. Brown, Stephan A. S. Alencar, Michael Ellis Oct 2020

Effect Of Drying And/Or Warming Piglets At Birth On Rectal Temperature Over The First 24 H After Birth, Katherine D. Vande Pol, Andres F. Tolosa, Caleb M. Shull, Catherine B. Brown, Stephan A. S. Alencar, Michael Ellis

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Piglets experience a rapid decrease in body temperature immediately after birth, increasing the risk of mortality. The objective of this study was to determine the effect of drying and/or warming piglets at birth on rectal temperature over the first 24 h after birth. The study was carried out at a commercial sow facility using a completely randomized design with four treatments (applied to piglets at birth): Control (no drying or warming), Desiccant (dried using a desiccant), Warming Box (placed in a box under a heat lamp for 30 min), and Desiccant + Warming Box (both dried and warmed as above). …