Meat Science Commons^™

Assessing The Impact Of Toxic Fescue Consumption During Gestation On Muscle Growth Gene Expression And Beef Color Quality Of The Offspring, Madison Blank

Animal Science Undergraduate Honors Theses

Endophyte-infected tall, toxic fescue is the most common forage for beef cattle in the southeastern U.S. However, exposure to this forage causes detrimental effects on the health of gestating cattle. The exposure to toxic fescue during gestation has been shown to impact fetal development during pregnancy and their offspring’s subsequent growth. However, limited information is currently available on the impact of this exposure on the meat quality of the offspring. Our objective is to determine how the dam’s exposure to toxic fescue during gestation impacts the beef color and quality of the final offspring.

A total of 22 animals (11 …

The Detection Of Morbidity Using A Remote Sensing Ear Tag On Newly Received Steers And Feedlot Performance And The Effects Of Feeding Experior And Optaflexx On Carcass Characteristics And Meat Tenderness In Beef Steers, Lauryn O. Smidt

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments were conducted to evaluate the effects of remote sensing health technology and growth-promoting feed additives on feedlot cattle performance, health outcomes, and carcass characteristics. In the first study, 638 crossbred steers (initial BW = 270 kg; SD = 18.6 kg) were used in a randomized block design to assess the effectiveness of a remote sensing ear tag (RST; HerdDogg, Seward, Nebraska) versus conventional pen-rider observation (PR) for detecting morbidity during a 35-d receiving period and subsequent 180-d finishing period. No differences were detected between PR and TAG for ending BW, average daily gain (ADG), dry matter intake (DMI), …

Carcass Mass And Cooling Dynamics As Determinants Of Beef Color, Tenderness, And Dark-Cutting Risk, Grace C. Johnson

Department of Animal Science: Dissertations, Theses, and Student Research

Discoloration in beef has important implications to the beef industry. Dark-cutting (DC) beef remains one of the most economically significant color defects; however, industry prevalence has only marginally declined (≈ 0.1%) despite mitigation efforts. This lack of improvement largely stems from research focused on pre-harvest stress and glycogen depletion leading to dark, firm, and dry (DFD) beef. However, not all darkcutting beef originates from DFD phenotypes. Atypical dark-cutting (ATDC) phenotypes have recently been observed and do not exhibit the classic metabolic indicators of DFD. This trend coincides with steadily increasing carcass weights in the beef industry and raises the possibility …

Lipid Deposition In Beef Cattle, Kyra L. Elliott

Department of Animal Science: Dissertations, Theses, and Student Research

Both the amount and types of lipid deposition in beef cattle are key determinants of carcass value and meat quality. Amounts of intramuscular, subcutaneous, and visceral fat influence carcass grading outcomes, palatability, and composition traits that are important to beef producers as well as consumers. The objectives of this thesis were to: 1) compare beef grading camera estimates of intramuscular fat to amounts of chemically extractable lipid, 2) characterize the lipidomes of beef intramuscular fat, subcutaneous fat, and lean muscle using shotgun lipidomics, and 3) identify and characterize a novel genetic variant in cattle that may have important implications for …

Application Of High-Pressure Processing To Improve The Color Of Dark Beef, Kara Ann Reynolds

Department of Animal Science: Dissertations, Theses, and Student Research

Deviation of beef from the bright cherry-red color preferred by consumers is often discounted or discarded at retail, resulting in substantial annual economic loss to the beef industry. One such deviation is dark cutting beef, which is characterized by a darker lean appearance that is undesirable to consumers and often rejected. Similarly, beef from mature cattle tends to exhibit a darker surface color, which can also reduce consumer acceptability. High-Pressure processing (HPP) has been investigated as a strategy to improve the color of dark cutting beef. Previous studies have focused on identifying pressure levels and holding times that optimize color …

Impact Of Feeding Palm Oil To Finishing Cattle On Performance, Carcass, And Meat Characteristics, Tate S. Johnson, Rebecca L. Mcdermott, Josh R. Benton, Galen E. Erickson, Jessie C. Morrill, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

This finishing study evaluated the effect of feeding palm oil products on performance and carcass characteristics, and beef quality measures. Dietary treatments included no supplemental fat or feeding: crude palm oil, crude high-oleic palm oil (HOPO), refined, bleached, and de-odorized palm olein oil (RBD olein), or tallow. Supplemental fat was included at 3.5% of diet dry matter. Cattle fed diets containing supplemental fat had improved feed conversion compared to no supplemental fat. Feeding cattle crude palm oil resulted in improved F:G compared to cattle fed RBD olein, with steers fed tallow and crude HOPO being intermediate and …

Carcass Weight Influences Chilling Rate, Postmortem Metabolism, And Beef Color, Grace C. Johnson, Mariane Beline, Leila G. Venzor, Kara A. Reynolds, Gary A. Sullivan, David E. Gerrad, Jordan C. Wicks

Nebraska Beef Cattle Reports

Summary with Implications

This study investigated the relationship between carcass weight and the development of atypical dark cutting (ATDC) beef by examining differences in chilling rates, muscle metabolism, and meat quality traits. Forty-four beef carcasses (heavy weight, HW, 510.17 ± 4.6 lb.; light weight, LW, 399.21 ± 4.6 lb.) were monitored postmortem for temperature, pH, and metabolite levels. LW carcasses cooled faster from 6 to 24 hours postmortem and exhibited higher pH at 12 hours, darker lean, and lower tenderness compared to HW carcasses. Elevated levels of glycogen and glucose-6-phosphate were observed in LW carcasses, suggesting that faster chilling may …

Impact Of Storage And Cooking Temperature On Listeria Innocua Growth In Plant-Based And Beef Proteins, Esther Vanoverbeke, Oliver Munezero, Gary A. Sullivan, Samodha C. Fernando

Nebraska Beef Cattle Reports

Summary with Implications

Listeriosis, caused by Listeria monocytogenes and other Listeria spp., is a food-borne illness, that accounts for 260 deaths annually. The objective of this study was to identify how storage method and cooking temperature impacts Listeria innocua growth in ground beef and plant-based protein patties. Patties (162 ground beef; 162 plant-based) were used in 3 replications. For each replication, patties were either inoculated (1×108 CFU) or not inoculated and stored for 26 hr at refrigeration, freezer, or room temperature. Three patties per storage method were cooked to either 145°F or 165°F and were plated before/after cooking. Nine patties …

Inclusion Of Palm Oil Products In Finishing Diets On Ground Beef Quality And Retail Stability, Anna Kobza, Galen E. Erickson, Jim C. Macdonald, Mitch L. Norman, Josh R. Benton, Rebecca L. Mcdermott, Long Zou, Kyra L. Elliott, Anna G. Hilton, Chad A. Russell, Jessie C. Morrill

Nebraska Beef Cattle Reports

Summary with Implications

Rising costs and availability of supplemental fats have increased interest in alternative fat sources in feedlot finishing diets, including palm oils. This experiment evaluated how refined, bleached, and deodorized (RBD) palm oil products included in the diet of feedlot finishing steers affect beef fat color and ground beef shelf-life. Steers were fed diets with no supplemental fat (No Oil), corn oil (Corn Oil), whole palm (Whole Palm), palm stearin (Stearin), or palm olein (Olein) at 4% dietary DM. Subcutaneous fat from cattle fed corn oil was more yellow than that from No Oil or palm oils. Ground …

Development Of A Shelf-Stable Proxy For Camera-Based Beef Grading Systems, Anna G. Hilton, Jessie C. Morrill

Nebraska Beef Cattle Reports

Summary with Implications

The American Meat Science Association's USDA Grading Committee recently expressed the need for a proxy device to verify that various camera grading systems produce consistent readings. To fulfill this need, we developed a proxy device made from 3D printed epoxy resin and other materials. The proxy was designed to have a known fat thickness and ribeye area. The proxy has a ribeye area of approximately 14 square inches and a fat thickness that ranges from 0.4 - 0.6 inches depending on the exact measurement location. The amount of marbling in the proxy can be adjusted incrementally and …

Prediction Of Intramuscular Fat Content Of Beef Longissimus Muscle, Kyra L. Elliott, Willy J. Horne, Bucky L. Gwartney, Jessie C. Morrill

Nebraska Beef Cattle Reports

Summary with Implications

In this study, we report the percentage of extractable intramuscular fat that is associated with beef longissimus muscle from a range of USDA marbling scores. The marbling scores were determined by two beef grading camera systems. We also assessed the utility of an open-source imaging software (ImageJ) for objectively quantifying the percentage of intramuscular fat, represented by pixels, in digital images of beef longissimus muscle.

Effect Of Nitrite Source & Reducing Compounds On Cured Color Development In An All-Beef Model System, Leila G. Venzor, Grace C. Johnson, Ashley A. Hahn, Benjamin L. Weinandt, Jordan C. Wicks, Gary A. Sullivan

Nebraska Beef Cattle Reports

Summary with Implications

This study evaluated the effects of nitrite source (sodium nitrite, celery powder, beet powder) and reducing agents (none, sodium erythorbate, acerola) on cured color development, cured meat pigment formation, and residual nitrite in an all-beef emulsion as a model system for frankfurters and smoked sausage. Treatments were prepared and analyzed for color, pigment concentration, and nitrite retention. Nitrite source did not significantly affect cured pigment or residual nitrite. The inclusion of reducing compounds significantly increased cured pigment and redness in cooked samples while lowering residual nitrite. No treatment differences were found for initial raw color. These results …

Application Of High-Pressure Processing Lightens The Color Of Cow Tenderloins, Kara A. Reynolds, Leila G. Venzor, Ashley A. Hahn, Grace C. Johnson, Jordan C. Wicks, Mary-Grace C. Danao, Ranjith Ramanathan, Gary A. Sullivan

Nebraska Beef Cattle Reports

Summary with Implications

This study evaluated the impact of high-pressure processing (HPP) on color, oxidative stability, and tenderness of tenderloins (psoas major) from cull cows to determine if HPP could enhance their marketability. Forty cull cow tenderloins were treated at two different HPP pressures and two different holding times, then assessed for instrumental and visual color, lipid oxidation, and Warner-Bratzler shear force during a 7-day stimulated shelf period. HPP increased initial lightness, especially at the highest pressure and longest holding time and improved redness initially. However, redness declined, and discoloration increased over the 7-day period, particularly after processing at higher …

Using High-Pressure Processing To Improve Dark-Cutting Beef Color In Tenderloins, Ashley A. Hahn, Leila G. Venzor, Shelley A. Curry, Grace C. Johnson, Mary-Grace C. Danao, Ranjith Ramanathan, Gary A. Sullivan, Jordan C. Wicks

Nebraska Beef Cattle Reports

Summary with Implications

Dark-cutting beef is a quality defect that negatively impacts consumer appeal and affects the carcass value for both producers and meat processors. These economic losses stem from poor visual appearance and reduced consumer appeal in the retail setting. Notably, different muscles within a carcass exhibit varying degrees of color stability which can influence how dark-cutting manifests across cuts. Therefore, this study aimed to examine the effect of high-pressure processing (HPP) on dark-cutting color-labile muscle. Dark-cutting and normal tenderloins were collected and portioned into two equal sections. Dark-cutting tenderloins were assigned one of 3 treatments: No HPP, 300 …

Defining The Parameters Of High-Pressure Processing To Improve The Quality Of Dark-Cutting Beef, Ashley A. Hahn, Leila G. Venzor, Shelley A. Curry, Grace C. Johnson, Mary-Grace C. Danao, Ranjith Ramanathan, Jordan C. Wicks, Gary A. Sullivan

Nebraska Beef Cattle Reports

Summary with Implications

Dark-cutting beef is a result of preharvest stress and devalues the resulting carcass. High-pressure processing (HPP) has been recently identified as a potential strategy to improve the undesirable appearance of dark-cutting beef. Dark-cutting beef strip loins were collected from a local processing facility, sectioned, and assigned an HPP pressure (300 or 450 MPa) and holding time (0, 1, 30, 60, or 90 s). Overall, HPP increased the lightness and redness of dark-cutting beef, particularly the longer the pressure was held at both pressure levels. Additionally, negative effects such as increased meat discoloration, decreased tenderness, and increased rancidity …

Are Midwest Region Extension Professionals Prepared To Answer Food Safety Questions About Various Meat Products?, Esther Vanoverbeke, Byron D. Chaves

Nebraska Beef Cattle Reports

Summary with Implications

Forty-eight million consumers in the U.S. get a foodborne illness each year; 128,000 are hospitalized and 3,000 die. Approximately one-third of those illnesses are related to animal-based foods, including meat. With meat-like alternatives being more readily available, it is critical to ensure these foods are handled appropriately to reduce foodborne illnesses. This project evaluated the food safety knowledge of extension professionals related to plant-based products and meat alternatives. A survey was distributed via the North Central Region Extension Center to Extension professionals (N=52). Most respondents, 58%, neither agreed or disagreed that plant-based meats/meat alternatives are microbiologically riskier …

2026 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

The 2026 Nebraska Beef Cattle Report brings together applied research, emerging technologies, and management insights that directly address the evolving challenges and opportunities facing today’s beef industry. This year’s collection includes 36 articles that span the full beef production system, from cow/calf and forage management to feedlot nutrition, animal health, behavior, meat quality, and sustainability, providing science-based guidance grounded in real-world production contexts.

Cow/Calf Nutrition, Reproduction, and Management

This section highlights management strategies that influence reproductive success and long-term herd performance. Articles focus on late-gestation heifer nutrition and practical applications of artificial insemination to support efficient, productive cow/calf systems.

Forage, …

One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika

All Peer-Reviewed Publications

The global human population is rapidly increasing, escalating interactions of people, animals and the environment. This has led to more convoluted health challenges, for which African smallholder farmers bear the brunt. The One Health (OH) approach recognises the interconnectedness of these health challenges and thus follows a transdisciplinary approach involving diverse stakeholders to address them. Although there have recently been global concerted efforts and studies on OH, an information gap exists in Africa, particularly regarding smallholder farmers and their needs. Additionally, evaluation frameworks and outcome metrics for OH initiatives are still unclear. Thus, this study was conducted to critically map …

Evaluating Predictive Models For Predicting Total Score Of Beef Carcasses, Emmanuel Forson

Electronic Theses and Dissertations

The beef industry plays a vital role in global agriculture, with carcass quality and consumer preference being key determinants of market success. This thesis examines predictive modeling techniques for estimating the Total Score of beef carcasses, a composite measure representing yield and quality, primarily used by the Nebraska Cattlemen Association. Using data from the Nebraska Cattlemen’s Foundation Retail Value Steer Challenge (2000–2023), the study compares the performance of First Order Multiple Linear Regression (MLR) with three machine learning techniques: K-Nearest Neighbors (KNN), Random Forest, and Gradient Boosting Machine (GBM).

The analysis focuses on six key predictors: Hot Carcass Weight, Back …

Artificial Insemination Of Beef Heifers With Multi-Sire Semen, Dempster Christenson, Jordan M. Thomas, Daniel J. Kelly, Richard N. Funston

Nebraska Beef Cattle Reports

This study compared pregnancy rates of beef heifers artificially inseminated with multi-sire semen to single-sire semen. It was hypothesized pregnancy rates resulting from multi-sire semen would be increased compared to single-sire semen. Heifers were artificially inseminated with semen from one of three single-sires or semen from a combination of the same three sires. Pregnancy rates did not significantly differ. Paternity testing suggests sire parentage can be unequal when semen is mixed from multiple sires. In summary, producers can expect similar pregnancy rates using single-sire and multi-sire semen, but progeny may have unequal sire representation.

Fat Deposition, Β- Carotene, And Vitamin A Metabolism In Bulls, Steers, And Heifers, Tesla Trammell

Masters Theses & Specialist Projects

Research highlights the impact of Vitamin A on cattle growth and meat quality. Supplementing Vitamin A early in life has been linked to enhanced IMF content and growth rates in calves, especially when administered at birth or during early developmental stages. This is attributed to the role of the ZFP423 gene in adipocyte formation, with studies noting its involvement in the development of IMF during the finishing phase.

This study aims to add to the present research by evaluate the effect of calves’ sexual class on blood β-carotene and Vitamin A before weaning. The second study evaluated the β- carotene …

Using High-Pressure Processing To Improve The Quality Of Dark-Cutting Beef, Ashley A. Hahn

Department of Animal Science: Dissertations, Theses, and Student Research

Previous studies have shown high-pressure processing (HPP) can be utilized to improve lean color of dark-cutting beef color by increasing the lightness and redness of steaks. However, the optimal parameters of length of time product is held under pressure and at which pressure must be applied have yet to be fully defined. Furthermore, the impact of HPP on color labile muscles in dark-cutting beef are currently unknown. Therefore, two studies were designed and specific efforts were focused on first defining optimal parameters for color stable muscles, followed by determining the effect of HPP on dark-cutting color labile muscles.

In the …

Managing The Beef Breeder Herd In Southern Western Australia, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Kilograms of beef produced per hectare is a significant driver of profit for a beef business. Cow longevity (length of productive life) plays an important role in achieving a profitable business. A balanced approach to genetic selection and nutritional management are essential factors underpinning the efficiency of the breeding herd.

Cattle Performance Grazing Canola And Cereal Crops, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

The Department of Primary Industries and Regional Development conducted research to investigate and demonstrate the performance of cattle grazing canola and cereal compared to animals left on pasture and investigate the value of spelling the pasture during the time the cattle are grazing crops.

Cattle grazing cereal crops had a higher liveweight gain compared to the cattle that remained on the pasture. However to see a significant benefit from grazing dual purpose crops, the grazing period may need to be longer than 4 weeks particularly when grazing canola.

The highest growth rates for cattle grazing the cereal crops were achieved …

Balancing The Scales: Evaluating Variables Of Greatest Impact To Profit Margins When Finishing Cattle, Ryan Bake

All Graduate Reports and Creative Projects, Fall 2023 to Present

This study examines the risk associated with finishing cattle in a feedlot, specifically in the context of historically high cattle prices and quality grade grid marketing systems that have not adjusted to these elevated prices. The analysis also looks at performance factors that drive profitability differences in pens of cattle in a commercial feedlot, and how market factors also impact profitability. Regression analysis determines the significance of performance variables such as days on feed, average daily gain, feed conversion rates, and carcass characteristics on pen profitability. The impact of cattle breeding and pre-feedlot management of the cattle are also considered …

Greenfeed Methane Measuring Machine, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

The Department of Primary Industries and Regional Development (DPIRD) now has the cutting-edge GreenFeed machine which measures emissions from individual cows and sheep, allowing us to determine Greenhouse Gas (GHG) emissions more accurately in the paddock.

The Effects Of Sous Vide Cooking And Cysteine Proteases On Instrumental And Sensory Textural Properties Of Lower Value Cuts Of Beef, Ian Smith

All Theses

Sous vide cooking has the potential to increase the value of lower quality cuts of beef. Understanding the textural properties following a cook-chill sous vide process is crucial to maximizing this potential. The use of enzymes may further increase the value of these products if able to positively alter the texture of the final product.

The first research objective was to use instrumental texture analysis to evaluate potential relationships between enzymatic treatments and sous vide processing time of lower value cuts of beef. Three cuts were evaluated: infraspinatus (top blade), semitendinosus (eye of round), and beef tongue. Infraspinatus and semitendinosus …

Measuring The Phytochemical Richness Of Meat: Effects Of Grass/Grain Finishing Systems And Grapeseed Extract Supplementation On The Fatty Acid And Phytochemical Content Of Beef, Lucas Krusinski, Isabella C. F. Maciel, Stephan Van Vliet, Muhammad Ahsin, Guanqi Lu, Jason E. Rowntree, Jenifer I. Fenton

Nutrition, Dietetics, and Food Sciences Faculty Publications

Grass-finished beef (GFB) can provide beneficial bioactive compounds to healthy diets, including omega-3 polyunsaturated fatty acids (n-3 PUFAs), conjugated linoleic acid (CLA), and secondary bioactive compounds, such as phytochemicals. The objective of this study was to compare fatty acids (FAs), micronutrients, and phytochemicals of beef fed a biodiverse pasture (GRASS), a total mixed ration (GRAIN), or a total mixed ration with 5% grapeseed extract (GRAPE). This was a two-year study involving fifty-four Red Angus steers (n = 54). GFB contained higher levels of n-3 PUFAs, vitamin E, iron, zinc, stachydrine, hippuric acid, citric acid, and succinic …

Texas A&M Beef Cattle Short Course: Program Evaluation, Nicola Oosthuizen, Pedro Fontes, Graham C. Lamb, Jason J. Cleere

Journal of Extension

Survey results (n = 3,748) collected over a period of 7 years from the Texas A&M Beef Cattle Short Course (BCSC) were analyzed to evaluate course demographics and the impact of the course on attendees. Results of this survey demonstrate that attendee demographics of the BCSC are representative of beef cattle producers in the United States and that the BCSC is effective at delivering information that positively impacts beef production in Texas. Extension professionals can make use of these findings to tailor future education programs to better serve the needs of beef cattle producers nation-wide.

How Do I Grow Tedera?, Daniel Real

Animal production and livestock published reports

The issue:

Livestock producers are faced with climate variability challenges and rising input costs while trying to maximise livestock productivity and profitability.

The impact:

Traditional or older pasture systems can limit production due to poor performance in dry periods or shorter production windows.

The opportunity:

Tedera is a resilient legume pasture that can fill feed gaps at a lower cost while offering livestock productivity gains.