Veterinary Infectious Diseases Commons^™

Pharmacokinetics Of Tulathromycin In Pregnant Ewes (Ovis Aries) Challenged With Campylobacter Jejuni, Michael Yaeger, Jonathan P. Mochel, Zuowei Wu, Paul Plummer, Orhan Sahin, Joseph Smith, Melda Ocal, Ashenafi Beyi, Changyun Xu, Qijing Zhang, Ronald W. Griffith

Faculty Publications and Other Works -- Large Animal Clinical Sciences

The purpose of this study was to evaluate the pharmacokinetics of tulathromycin in the plasma and maternal and fetal tissues of pregnant ewes when administered within 24 hours of a single, IV Campylobacter jejuni (C. jejuni) challenge. Twelve, pregnant ewes between 72–92 days of gestation were challenged IV with C. jejuni IA3902 and then treated with 1.1 ml/45.36 kg of tulathromycin subcutaneously 18 hours post-challenge. Ewes were bled at predetermined time points and euthanized either at a predetermined time point or following the observation of vaginal bleeding or abortion. Following euthanasia, tissues were collected for bacterial ...

First Molecular Detection Of Hepatitis E Virus Genome In Camel And Pig Faecal Samples In Ethiopia, Fufa Dawo Bari, Haimanot Belete Wodaje, Umar Said, Hika Waktole, Melaku Sombo, Samson Leta, Tesfaye Rufael Chibsa, Paul J. Plummer

Veterinary Diagnostic and Production Animal Medicine Publications

Background: Hepatitis E is an enteric and zoonotic disease caused by hepatitis E virus (HEV) that is mainly transmitted via the faecal-oral route through contaminated food or the environment. The virus is an emerging infectious agent causing acute human infection worldwide. A high seroprevalence of the disease was reported in pregnant women in Addis Ababa, Ethiopia, raising significant public health concern. The presence of HEV specific antibodies were also reported in dromedary camels in the country; however, the infectious virus and/or the viral genome have not been demonstrated to date in animal samples.

Methods: To address this gap, a ...

Self-Assembling Synthetic Nanoadjuvant Scaffolds Cross-Link B Cell Receptors And Represent New Platform Technology For Therapeutic Antibody Production, Sujata Senapati, Ross J. Darling, Kathleen A. Ross, Michael J. Wannemeuhler, Balaji Narasimhan, Surya K. Mallapragada

Chemical and Biological Engineering Publications

Host antibody responses are pivotal for providing protection against infectious agents. We have pioneered a new class of self-assembling micelles based on pentablock copolymers that enhance antibody responses while providing a low inflammatory environment compared to traditional adjuvants. This type of “just-right” immune response is critical in the rational design of vaccines for older adults. Here, we report on the mechanism of enhancement of antibody responses by pentablock copolymer micelles, which act as scaffolds for antigen presentation to B cells and cross-link B cell receptors, unlike other micelle-forming synthetic block copolymers. We exploited this unique mechanism and developed these scaffolds ...

Investigating The Relationship Of Porcine Reproductive And Respiratory Syndrome Virus Rna Detection Between Adult/Sow Farm And Wean-To-Market Age Categories, Yiqun Jiang, Qing Li, Giovani Trevisan, Daniel C. L. Linhares, Cameron A. Mackenzie

Veterinary Diagnostic and Production Animal Medicine Publications

Porcine reproductive and respiratory syndrome (PRRS) is a disease caused by the PRRS virus (PRRSV) that has spread globally in the last 30 years and causes huge economic losses every year. This research aims to 1) investigate the relationship between the PRRSV detection in two age categories (wean-to-market and adult/sow farm), and 2) examine the extent to which the wean-to-market PRRSV positive rate forecasts the adult/sow farm PRRSV positive rate. The data we used are the PRRSV RNA detection results between 2007 and 2019 integrated by the US Swine Disease Reporting System project that represent 95% of all ...

Complete Coding Genome Sequence Of A Novel Porcine Reproductive And Respiratory Syndrome Virus 2 Restriction Fragment Length Polymorphism 1-4-4 Lineage 1c Variant Identified In Iowa, Usa, Giovani Trevisan, Ganwu Li, Cesar A.A. Moura, Katie Coleman, Pete Thomas, Jianqiang Zhang, Phillip Gauger, Michael Zeller, Daniel C.L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

A porcine reproductive and respiratory syndrome virus 2 strain was identified in lung samples from nursery piglets associated with a 17.15% mortality rate on a swine farm in Iowa. Open reading frame 5 (ORF5) sequencing indicated that this strain is a restriction fragment length polymorphism (RFLP) 1-4-4 lineage 1C variant strain, and its complete coding genome sequence was determined.

Comparison Of Mycoplasma Hyopneumoniae Response To Infection By Route Of Exposure, Ana Paula S. Poeta Silva, Thaire P. Marostica, Aric Mcdaniel, Bailey L. Arruda, Carmen Alonso, Rachel J. Derscheid, Paul Yeske, Daniel C.L. Linhares, Luis Giménez-Lirola, Locke A. Karriker, Eduardo Fano, Jeffrey J. Zimmerman, Maria J. Clavijo

Veterinary Diagnostic and Production Animal Medicine Publications

Mycoplasma hyopneumoniae (MHP) is a concern both for pig well-being and producer economic viability. In the absence of fully protective health interventions, producers rely on controlled exposure to induce an immune response in pigs and minimize the clinical outcomes of MHP infection in pig populations. This study compared the effect of route of exposure on MHP infection, antibody response, clinical signs, and pathology. Six-week-old MHP-negative pigs (n = 78) were allocated to negative control (n = 6) or one of three MHP exposure routes: intratracheal (n = 24, feeding catheter), intranasal (n = 24, atomization device), and aerosol (n = 24, fogger). Body weight ...

Designing And Testing Of A System For Aerosolization And Recovery Of Viable Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): Theoretical And Engineering Considerations, Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Steven J. Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, Myeongseong Lee, Baitong Chen, William S. Jenks

Agricultural and Biosystems Engineering Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) infections cause significant economic losses to swine producers every year. Aerosols containing infectious PRRSV are an important route of transmission, and proper treatment of air could mitigate the airborne spread of the virus within and between barns. Previous bioaerosol studies focused on the microbiology of PRRSV aerosols; thus, the current study addressed the engineering aspects of virus aerosolization and collection. Specific objectives were to (1) build and test a virus aerosolization system, (2) achieve a uniform and repeatable aerosol generation and collection throughout all replicates, (3) identify and minimize sources of variation, and ...

Determination Of Ownership Practices And Cytauxzoon Felis Prevalence In Domestic Felines Of Northwest Arkansas, Daniella M. Duran

Animal Science Undergraduate Honors Theses

Cytauxzoon felis is a protozoan pathogen that causes the infectious disease, cytauxzoonosis in wild and domestic felines. The natural host of this pathogen is the bobcat (Lynx rufus), which is why this disease is also commonly referred to as “bobcat fever”. The C. felis pathogen infects wild and domestic cats upon transmission by infected ticks. Amblyomma americanum, also known as the Lone Star tick, the primary transmission vector for C. felis. This tick species is largely populated throughout the state of Arkansas, making C. felis a large threat to wild and domestic felines in these areas. Cytauxzoonosis is commonly thought ...

Production Of Monoclonal And Polyclonal Antibodies Against Prrsv Mlvd23-S Tag Peptide Sequence, Katelyn Bettin

Schultz-Werth Award Papers

Porcine reproductive and respiratory syndrome virus (PRRSV) is an RNA virus which belongs to the family Arteriviridae. Since its emergence in the late 1980’s and early 1990’s, PRRSV has had a major economic effect on the swine industry. The most notable effects of this disease are respiratory distress in piglets and late-term pregnancy failures in sows. To track and control spread of this disease, the industry must be able determine which animals have been naturally infected with PRRSV as this contributes to major disease outbreaks and localized pandemics. While vaccines help control the spread of a disease, they ...

Longitudinal Piglet Sampling In Commercial Sow Farms Highlights The Challenge Of Prrsv Detection, Marcelo Nunes De Almeida, Cesar A. Corzo, Jeffrey J. Zimmerman, Daniel C. L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

Background: Processing fluids (PF) and family oral fluids (FOF) are population-based surveillance samples collected from 2- to 5-day-old piglets and due-to-wean piglets, respectively. Although they are described for the surveillance of PRRSV in sows and piglet populations at processing and weaning, there is limited information on their use in commercial herds. This observational study described PRRSV RNA detection over time in PF, FOF, and piglet serum collected from farrowing groups in commercial breeding farms with the objective of achieving robust, practical, and effective PRRSV surveillance protocols. Weekly PF (an aggregate sample of all litters processed in a week from each ...

Using Enclosed Y-Mazes To Assess Chemosensory Behavior In Reptiles, M. Rockwell Parker, Andrea F. Currylow, Eric A. Tillman, Charlotte J. Robinson, Jilian M. Josimovich, Isabella M.G. Bukovich, Lauren A. Nazarian, Melia G. Nafus, Bryan M. Kluever, Amy A. Yackel Adams

USDA Wildlife Services - Staff Publications

Reptiles utilize a variety of environmental cues to inform and drive animal behavior such as chemical scent trails produced by food or conspecifics. Decrypting the scent-trailing behavior of vertebrates, particularly invasive species, enables the discovery of cues that induce exploratory behavior and can aid in the development of valuable basic and applied biological tools. However, pinpointing behaviors dominantly driven by chemical cues versus other competing environmental cues can be challenging. Y-mazes are common tools used in animal behavior research that allow quantification of vertebrate chemosensory behavior across a range of taxa. By reducing external stimuli, Y-mazes remove confounding factors and ...

Genomics Of Response To Prrsv In Purebred And Crossbred Sows: Antibody Response And Performance Following Natural Infection Versus Vaccination, Leticia P. Sanglard, Felipe M. W. Hickmann, Yijian Huang, Kent A. Gray, Daniel C. L. Linhares, Jack C. M. Dekkers, Megan C. Niederwerder, Rohan L. Fernando, Joseph Braccini Neto, Nick V. L. Serão

Veterinary Diagnostic and Production Animal Medicine Publications

Antibody response, measured as sample-to-positive (S/P) ratio, to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) following a PRRSV-outbreak (S/POutbreak) in a purebred nucleus and following a PRRSV-vaccination (S/PVx) in commercial crossbred herds have been proposed as genetic indicator traits for improved reproductive performance in PRRSV-infected purebred and PRRSV-vaccinated crossbred sows, respectively. In this study, we investigated the genetic relationships of S/POutbreak and S/PVx with performance at the commercial (vaccinated crossbred sows) and nucleus level (non-infected and PRRSV-infected purebred sows), respectively, and tested the effect of previously identified SNP for these indicator traits. Antibody response was ...

Data Standardization Implementation And Applications Within And Among Diagnostic Laboratories: Integrating And Monitoring Enteric Coronaviruses, Giovani Trevisan, Leticia C. M. Linhares, Kent J. Schwartz, Eric R. Burrough, Edison De S. Magalhães, Bret Crim, Poonam Dubey, Rodger G. Main, Phillip C. Gauger, Mary Thurn, Paulo T. F. Lages, Cesar A. Corzo, Jerry Torrison, Jamie Henningson, Eric Herrman, Rob Mcgaughey, Giselle Cino, Jon Greseth, Travis Clement, Jane Christopher-Hennings, Daniel C. L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

Every day, thousands of samples from diverse populations of animals are submitted to veterinary diagnostic laboratories (VDLs) for testing. Each VDL has its own laboratory information management system (LIMS), with processes and procedures to capture submission information, perform laboratory tests, define the boundaries of test results (i.e., positive or negative), and report results, in addition to internal business and accounting applications. Enormous quantities of data are accumulated and stored within VDL LIMSs. There is a need for platforms that allow VDLs to exchange and share portions of laboratory data using standardized, reliable, and sustainable information technology processes. Here we ...

Mitigation Of Airborne Prrsv Transmission With Uv Light Treatment: Proof-Of-Concept, Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, William S. Jenks, Myeongseong Lee, Baitong Chen, Steven J. Hoff

Agricultural and Biosystems Engineering Publications

Proper treatment of infectious air could potentially mitigate the spread of airborne viruses such as porcine reproductive and respiratory syndrome virus (PRRSV). The objective of this research is to test the effectiveness of ultraviolet (UV) in inactivating aerosolized PRRSV, specifically, four UV lamps, UV-A (365 nm, both fluorescent and LED-based), “excimer” UV-C (222 nm), and germicidal UV-C (254 nm), were tested. The two UV-C lamps effectively irradiated fast-moving PRRSV aerosols with short treatment times (<2 s). One-stage and two-stage UV inactivation models estimated the UV doses needed for target percentage (%) reductions on PRRSV titer. UV-C (254 nm) dose needed for 3-log (99.9%) reduction was 0.521 and 0.0943 mJ/cm², respectively, based on one-stage and two-stage models. An order of magnitude lower UV-C (222 nm) doses were needed for a 3-log reduction, i.e., 0 ...

Distribution Of Crispr Types In Fluoroquinolone-Resistant Campylobacter Jejuni Isolates, Mehmet Cemal Adiguzel, Debora Brito Goulart, Zuowei Wu, Jinji Pang, Seyda Cengiz, Qijing Zhang, Orhan Sahin

Veterinary Microbiology and Preventive Medicine Publications

To aid development of phage therapy against Campylobacter, we investigated the distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) systems in fluoroquinolone (FQ)-resistant Campylobacter jejuni. A total of 100 FQ-resistant C. jejuni strains from different sources were analyzed by PCR and DNA sequencing to determine resistance-conferring mutation in the gyrA gene and the presence of various CRISPR systems. All but one isolate harbored 1–5 point mutations in gyrA, and the most common mutation was the Thr86Ile change. Ninety-five isolates were positive with the CRISPR PCR, and spacer sequences were found in 86 of them. Among the ...

Pioneer Science And The Great Plagues: How Microbes, War, And Public Health Shaped Animal Health, Norman F. Cheville, Purdue University Press

Purdue University Press Books

Pioneer Science and the Great Plagues covers the century when infectious plagues—anthrax, tuberculosis, tetanus, plague, smallpox, and polio—were conquered, and details the important role that veterinary scientists played. The narrative is driven by astonishing events that centered on animal disease: the influenza pandemic of 1872, discovery of the causes of anthrax and tuberculosis in the 1880s, conquest of Texas cattle fever and then yellow fever, German anthrax attacks on the United States during World War I, the tuberculin war of 1931, Japanese biological warfare in the 1940s, and today’s bioterror dangers.

Veterinary science in the rural Midwest ...

Sars-Cov-2 Exposure In Escaped Mink, Utah, Usa, Susan A. Shriner, Jeremy W. Ellis, J. Jeffrey Root, Annette Roug, Scott R. Stopak, Gerald W. Wiscomb, Jared R. Zierenberg, Hon S. Ip, Mia Kim Torchetti, Thomas J. Deliberto

USDA Wildlife Services - Staff Publications

In August 2020, outbreaks of coronavirus disease were confirmed on mink farms in Utah, USA. We surveyed mammals captured on and around farms for evidence of infection or exposure. Free-ranging mink, presumed domestic escapees, exhibited high antibody titers, suggesting a potential severe acute respiratory syndrome coronavirus 2 transmission pathway to native wildlife.

We report a wildlife epidemiologic investigation of mammals captured on or near properties in Utah, USA, where outbreaks of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection occurred in farmed mink. Mink farms are relatively common in the United States, and most are small family farms. The US ...

Toxicity Of Sodium Nitrite-Based Vertebrate Pesticides For European Starlings (Sturnus Vulgaris), Scott J. Werner, Shelagh T. Deliberto, Hailey E. Mclean, Katherine E. Horak, Kirt C. Vercauteren

USDA Wildlife Services - Staff Publications

In the 21st century, invasive animals rank second only to habitat destruction as the greatest threat to global biodiversity. Socially-acceptable and cost-effective strategies are needed to reduce the negative economic and environmental impacts of invasive animals. We investigated the potential for sodium nitrite (SN; CAS 7632-00-0) to serve as an avian toxicant for European starlings (Sturnus vulgaris L.). We also assessed the non-target hazard of an experimental formulation of SN that is being developed as a toxicant for invasive wild pigs (Sus scrofa L.). In gavage experiments with European starlings, we identified a lowest observed adverse effect level (LOAEL) for ...

Great Expectations: Deconstructing The Process Pathways Underlying Beaver-Related Restoration, Caroline S. Nash, Gordon E. Grant, Susan Charnley, Jason B. Dunham, Hannah Gosnell, Mark B. Hausner, David S. Pilliod, Jimmy Taylor

USDA Wildlife Services - Staff Publications

Beaver-related restoration is a process-based strategy that seeks to address wide-ranging ecological objectives by reestablishing dam building in degraded stream systems. Although the beaver-related restoration has broad appeal, especially in water-limited systems, its effectiveness is not yet well documented. In this article, we present a process-expectation framework that links beaver-related restoration tactics to commonly expected outcomes by identifying the set of process pathways that must occur to achieve those expected outcomes. We explore the contingency implicit within this framework using social and biophysical data from project and research sites. This analysis reveals that outcomes are often predicated on complex process ...

Effect Of Prrsv Stability On Productive Parameters In Breeding Herds Of A Swine Large Integrated Group In Spain, D. Torrents, J. Miranda, Phillip C. Gauger, Alejandro Ramirez, Daniel C. L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

Background In breeding herds, porcine reproductive and respiratory syndrome (PRRS) clinically manifests as increased abortions, number of stillbirths, and pre-weaning mortality, and as a direct consequence, results in a decrease of the number of piglets weaned per sow per year. Breeding farm classification according the PRRS virus (PRRSV) status (unstable or stable) is a key control strategy for this disease. The aim of this study was to evaluate the production improvement related to achieving a PRRSV stable status in breeding herds in Spain. For this purpose, epidemiological and productivity data were collected from a systematic PRRSV monitoring program in 35 ...