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Detection Of Salmonella Enteritidis In Pooled Poultry Environmental Samples Using A Serotype-Specific Real-Time–Polymerase Chain Reaction Assay, Derek R. Adams, Wendy R. Stensland, Chong Wang, Annette O'Connor, Darrell W. Trampel, Karen M. Harmon, Erin L. Strait, Timothy S. Frana 2017 Iowa State University

Detection Of Salmonella Enteritidis In Pooled Poultry Environmental Samples Using A Serotype-Specific Real-Time–Polymerase Chain Reaction Assay, Derek R. Adams, Wendy R. Stensland, Chong Wang, Annette O'Connor, Darrell W. Trampel, Karen M. Harmon, Erin L. Strait, Timothy S. Frana

Annette O'Connor

While real-time–polymerase chain reaction (RT PCR) has been used as a rapid test for detection of Salmonella Enteritidis in recent years, little research has been done to assess the feasibility of pooling poultry environmental samples with aSalmonella Enteritidis–specific RT PCR assay. Therefore the objective of this study was to compare RT PCR SalmonellaEnteritidis detection in individual and pooled (in groups of two, three, and four) poultry environmental drag swab samples to traditional cultural methods. The drag swabs were collected from poultry facilities previously confirmed positive forSalmonella Enteritidis and were cultured according to National Poultry Improvement Plan guidelines. Initial ...


Endocrine And Metabolic Effects Of Consuming Sugar-Sweetened Beverages: Preclinical And Clinical Studies", Peter Havel 2017 University of California, Davis

Endocrine And Metabolic Effects Of Consuming Sugar-Sweetened Beverages: Preclinical And Clinical Studies", Peter Havel

Science Seminar Series

Peter J. Havel of the School of Veterinary Medicine at UC Davis will speak on his research on Endocrine and Metabolic Effects of Consuming Sugar-Sweetened Beverages: Preclinical and clinical studies at this Science Seminar Series lecture.


Effects Of Temperature And Diet In Stable Fly (Diptera: Muscidae) Development, Melina Florez-Cuadros 2017 University of Nebraska-Lincoln

Effects Of Temperature And Diet In Stable Fly (Diptera: Muscidae) Development, Melina Florez-Cuadros

Dissertations and Student Research in Entomology

Stable flies are among the most important blood feeding ectoparasites of cattle worldwide. The livestock industry is losing more than $2 billion dollar annually in the United States alone. Moist decaying vegetative material with bacteria supports larval development. Temperature and diet quality are important factors for stable fly development, affecting development rates, adult survival, and size, but how temperature affects adult size and interacts with nutrition is unknown. This study characterizes the relationship of temperature and diet quality in the development of stable flies. Two statistical models were used to analyze the effect of temperature alone (Lysyk, 1998) and temperature-diet ...


Assessing Readiness For Clinical Practice: Students’ Perspectives Of Their Veterinary Curriculum, L. Chris Sanchez, Alison Kwiatkowski, Jeff Abbott, Dana N. Zimmel, Linda S. Behar-Horenstein 2016 University of Florida College of Veterinary Medicine

Assessing Readiness For Clinical Practice: Students’ Perspectives Of Their Veterinary Curriculum, L. Chris Sanchez, Alison Kwiatkowski, Jeff Abbott, Dana N. Zimmel, Linda S. Behar-Horenstein

The Qualitative Report

Studies describing the effectiveness of a veterinary curriculum from the student perspective are currently sparse. The overall purpose of this investigation was to describe students’ perceived preparedness for clinical practice. Three focus group meetings with fourth year veterinary students were conducted. Data were open-coded and categorized to identify themes. Four main themes emerged: Challenging communications, Un/appreciating curricular experiences, Documenting demands impede case involvement, and Hungering for timely, effective feedback. Overall students felt comfortable talking to clients about medicine but less comfortable discussing euthanasia or money; they appreciated the split clinical curriculum but questioned the value of the 1st/2nd ...


Surveillance, Epidemiological, And Virological Detection Of Highly Pathogenic H5n1 Avian Influenza Viruses In Duck And Poultry From Bangladesh, Wahedul Karim Ansari, Md Safiullah Parvej, Mohamed E. El Zowalaty, Sally Jackson, Stephen A. Bustin, Adel K. Ibrahim, Md Tanvir Rahman, Han Zhang, Mohammad Ferdousur Rahman Khan, Md Mostakin Ahamd, Md. Fasiur Rahman, Marzia Rahman, KHM Nazmul H. Nazir, Sultan Ahmed, Md Liakot Hossenn, Md Abdul Kafi, Mat Yamage, Nitish C. Debnath, Graba Ahmed, Hossam Ashour, Md Masoud, Ayman Noreddin, Md B. Rahman 2016 Bangladesh Agricultural University

Surveillance, Epidemiological, And Virological Detection Of Highly Pathogenic H5n1 Avian Influenza Viruses In Duck And Poultry From Bangladesh, Wahedul Karim Ansari, Md Safiullah Parvej, Mohamed E. El Zowalaty, Sally Jackson, Stephen A. Bustin, Adel K. Ibrahim, Md Tanvir Rahman, Han Zhang, Mohammad Ferdousur Rahman Khan, Md Mostakin Ahamd, Md. Fasiur Rahman, Marzia Rahman, Khm Nazmul H. Nazir, Sultan Ahmed, Md Liakot Hossenn, Md Abdul Kafi, Mat Yamage, Nitish C. Debnath, Graba Ahmed, Hossam Ashour, Md Masoud, Ayman Noreddin, Md B. Rahman

Pharmacy Faculty Articles and Research

Avian influenza viruses (AIVs) continue to pose a global threat. Waterfowl are the main reservoir and are responsible for the spillover of AIVs to other hosts. This study was conducted as part of routine surveillance activities in Bangladesh and it reports on the serological and molecular detection of H5N1 AIV subtype. A total of 2169 cloacal and 2191 oropharyngeal swabs as well as 1725 sera samples were collected from live birds including duck and chicken in different locations in Bangladesh between the years of 2013 and 2014. Samples were tested using virus isolation, serological tests and molecular methods of RT-PCR ...


Carbon Dioxide For Euthanasia: Concerns Regarding Pain And Distress, With Special Reference To Mice And Rats, Kathleen Conlee, Martin Stephens, Andrew N. Rowan, Lesley A. King 2016 The Humane Society of the United States

Carbon Dioxide For Euthanasia: Concerns Regarding Pain And Distress, With Special Reference To Mice And Rats, Kathleen Conlee, Martin Stephens, Andrew N. Rowan, Lesley A. King

Martin Stephens, Ph.D.

Carbon dioxide (CO2) is the most commonly used agent for euthanasia of laboratory rodents, used on an estimated tens of millions of laboratory rodents per year worldwide, yet there is a growing body of evidence indicating that exposure to CO2 causes more than momentary pain and distress in these and other animals. We reviewed the available literature on the use of CO2 for euthanasia (as well as anaesthesia) and also informally canvassed laboratory animal personnel for their opinions regarding this topic. Our review addresses key issues such as CO2 flow rate and final concentration, presence of oxygen, and prefilled chambers ...


A Novel Educational Tool For Teaching Diagnostic Reasoning And Laboratory Data Interpretation To Veterinary (And Medical) Students., Holly Bender, Jared A. Danielson 2016 Iowa State University

A Novel Educational Tool For Teaching Diagnostic Reasoning And Laboratory Data Interpretation To Veterinary (And Medical) Students., Holly Bender, Jared A. Danielson

Holly Bender

The Diagnostic Pathfinder was designed to help students learn diagnostic problem solving by supporting them in explaining relationships among history and physical examination findings, data abnormalities, and the underlying mechanisms of disease. The Pathfinder has been used to teach diagnostic problem solving to veterinary students since 2001 and is currently in use at 10 colleges of veterinary medicine. This article describes how the Pathfinder works and summarizes results from studies exploring the effect of Pathfinder use on learning and satisfaction. Pathfinder characteristics are described in terms of their influence on cognitive load, and strategies are provided for effective implementation.


Purification And Characterization Of A Fish Granzymea Involved In Cell-Mediated Immunity, Yuta Matsuura, Takeshi Yabu, Hajime Shiba, Teruyuki Nakanishi 2016 Nihon University

Purification And Characterization Of A Fish Granzymea Involved In Cell-Mediated Immunity, Yuta Matsuura, Takeshi Yabu, Hajime Shiba, Teruyuki Nakanishi

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Expansion, Characterization, Differentiation, And Visualization Of Mc 3t3-E1 Preosteoblast Cells: An In Vitro Model To Study Bone Healing And Stem Cell-Mediated Regeneration, Jakob T. Samsel, Madhu Dhar, Austin Bow, Tom Masi 2016 University of Tennessee, Knoxville

Expansion, Characterization, Differentiation, And Visualization Of Mc 3t3-E1 Preosteoblast Cells: An In Vitro Model To Study Bone Healing And Stem Cell-Mediated Regeneration, Jakob T. Samsel, Madhu Dhar, Austin Bow, Tom Masi

University of Tennessee Honors Thesis Projects

No abstract provided.


Persistent Behavior In Domestic Canines (Canis Familiaris), Megan Arant 2016 Carroll College - Helena

Persistent Behavior In Domestic Canines (Canis Familiaris), Megan Arant

Anthrozoology Undergraduate Theses

Behavioral persistence is the continuation of responding without reward. (Weiner, H. 1970) It is important to look at persistence in domestic dog behavior so people can better partner with them professionally in day to day life. Working dogs, such as service dogs and police dogs, often have to learn to work under conditions involving little or no reinforcement or delayed reinforcement conditions. To make training more effective for these working dogs understanding the factors that influence the persistence aspects would be important. Researching persistence also has an application with owned pet dogs, for example, owners may not want to rely ...


Genetic Control Of Immune Response To Pseudorabies And Atrophic Rhinitis Vaccines: Ii: Comparison Of Additive Direct And Maternal Genetic Effects, David Lynn Meeker, Max F. Rothschild, L. L. Christian, C. M. Warner, H. T. Hill 2016 Iowa State University

Genetic Control Of Immune Response To Pseudorabies And Atrophic Rhinitis Vaccines: Ii: Comparison Of Additive Direct And Maternal Genetic Effects, David Lynn Meeker, Max F. Rothschild, L. L. Christian, C. M. Warner, H. T. Hill

Max Rothschild

Data from 52 litters farrowed in two seasons of a cross-fostering experiment were analyzed to estimate variances and covariances for additive direct and maternal genetic effects on immune response to pseudorabies virus and B. bronchiseptica vaccine. Twenty purebred boars and 44 sows of the Duroc, Landrace and Yorkshire breeds were used. Immune response was measured after vaccine challenge. A modified-live pseudorabies (PR) vaccine was administered to piglets at 28 d of age; response was measured by log, serum neutralization titers at 56 d. An inactivated B. bronchiseptica bacterin was administered at 28, 42 and 112 d. Antibody levels were measured ...


Impact Of Carprofen Administration On Stress And Nociception Responses Of Calves To Cautery Dehorning, M. L. Stock, L. A. Barth, Nicholas K. Van Engen, Suzanne T. Millman, R. Gehring, Chong Wang, E. A. Voris, Larry W. Wulf, Lea Labeur, Walter H. Hsu, Johann F. Coetzee 2016 Iowa State University

Impact Of Carprofen Administration On Stress And Nociception Responses Of Calves To Cautery Dehorning, M. L. Stock, L. A. Barth, Nicholas K. Van Engen, Suzanne T. Millman, R. Gehring, Chong Wang, E. A. Voris, Larry W. Wulf, Lea Labeur, Walter H. Hsu, Johann F. Coetzee

Veterinary Diagnostic and Production Animal Medicine Publications

The objective of this study was to investigate the effects of carprofen administered immediately before cautery dehorning on nociception and stress. Forty Holstein calves aged approximately 6 to 8 wk old were either placebo treated and sham dehorned (n = 10) or cautery dehorned following administration of carprofen (1.4 mg/kg) subcutaneously (n = 10) or orally (n = 10) or a subcutaneous and oral placebo (n = 10) in a randomized, controlled trial. All animals were given a cornual nerve block using lidocaine before dehorning. Response variables including mechanical nociception threshold, ocular temperature, heart rate, and respiratory rate were measured before and ...


Retinal Detachment Post-Phacoemulsification In Bichons Frises: A Retrospective Study Of 54 Dogs, Silvia G. Pryor, Ellison Bentley, Gillian J. McLellan, Elizabeth A. Giuliano, Rachel A. Allbaugh, Amy J. Rankin, Amber L. Labelle, Kevin A. Buhr 2016 University of Wisconsin-Madison

Retinal Detachment Post-Phacoemulsification In Bichons Frises: A Retrospective Study Of 54 Dogs, Silvia G. Pryor, Ellison Bentley, Gillian J. Mclellan, Elizabeth A. Giuliano, Rachel A. Allbaugh, Amy J. Rankin, Amber L. Labelle, Kevin A. Buhr

Veterinary Clinical Sciences Publications

Objective—To compare rates of retinal detachment (RD) post phacoemulsification in American Bichons Frises with and without prophylactic retinopexy.

Procedures—Medical records of 54 Bichons Frises undergoing phacoemulsification with or without prophylactic retinopexy between 2003–2013 in one or both eyes were reviewed from five Midwestern university veterinary teaching hospitals. Inclusion criteria were pre-operative ERG, at least 6 months of follow up post phacoemulsification, and absence of pre-existing RD as determined by ophthalmic examination and/or ultrasound. Statistical analyses used chi-squared and Wilcoxon rank-sum tests and Wilson confidence intervals with the p value

Results—Phacoemulsification was performed without retinopexy in ...


Exploring Attitudes Toward Euthanasia Among Shelter Workers And Volunteers In Japan And The U.S., Maho Cavalier 2016 Humane Society University

Exploring Attitudes Toward Euthanasia Among Shelter Workers And Volunteers In Japan And The U.S., Maho Cavalier

STU

Conflicts between foreign and Japanese volunteers concerning decisions to euthanize animals that are in terminal condition were noted during anecdotal observation by this author at a Japanese animal rescue shelter. Thus, this research was undertaken to explore attitudes of shelter staff in Japan. As a point of comparison, U. S. shelter workers were also interviewed. Twenty current or former shelter workers (16 participants from Japan and 4 participants from the U.S.), ranging in age from 20 to 65, were interviewed. Face-to-face interviews were conducted with the Japanese participants; e-mail and/or Skype were used to conduct the interviews with ...


Ring Test Evaluation Of The Detection Of Influenza A Virus In Swine Oral Fluids By Real-Time Reverse-Transcription Polymerase Chain Reaction And Virus Isolation, Christa K. Goodell, Jianqiang Zhang, Erin Strait, Karen Harmon, Devi Patnayak, Tracy Otterson, Marie Culhane, Jane Christopher-Hennings, Travis Clement, Pamela Leslie-Steen, Richard Hesse, Joe Anderson, Kevin Skarbek, Amy Vincent, Pravina Kitikoon, Sabrina Swenson, Melinda Jenkins-Moore, Jodi McGill, Rolf Rauh, William Nelson, Catherine O'Connell, Rohn Shah, Chong Wang, Rodger Main, Jeffery J. Zimmerman 2016 Iowa State University

Ring Test Evaluation Of The Detection Of Influenza A Virus In Swine Oral Fluids By Real-Time Reverse-Transcription Polymerase Chain Reaction And Virus Isolation, Christa K. Goodell, Jianqiang Zhang, Erin Strait, Karen Harmon, Devi Patnayak, Tracy Otterson, Marie Culhane, Jane Christopher-Hennings, Travis Clement, Pamela Leslie-Steen, Richard Hesse, Joe Anderson, Kevin Skarbek, Amy Vincent, Pravina Kitikoon, Sabrina Swenson, Melinda Jenkins-Moore, Jodi Mcgill, Rolf Rauh, William Nelson, Catherine O'Connell, Rohn Shah, Chong Wang, Rodger Main, Jeffery J. Zimmerman

Veterinary Diagnostic and Production Animal Medicine Publications

The probability of detecting influenza A virus (IAV) in oral fluid (OF) specimens was calculated for each of 13 assays based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) and 7 assays based on virus isolation (VI). The OF specimens were inoculated with H1N1 or H3N2 IAV and serially diluted 10-fold (10(-1) to 10(-8)). Eight participating laboratories received 180 randomized OF samples (10 replicates × 8 dilutions × 2 IAV subtypes plus 20 IAV-negative samples) and performed the rRT-PCR and VI procedure(s) of their choice. Analysis of the results with a mixed-effect logistic-regression model identified dilution and assay as variables ...


In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, Kyong-Sup Yoon, Jennifer K. Ketzis, Samuel W. Andrewes, Christopher S. Wu, Kris Honraet, Dorien Staljanssens, Bart Rossel, J Marshall Clark 2015 Southern Illinois University Edwardsville

In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, Kyong-Sup Yoon, Jennifer K. Ketzis, Samuel W. Andrewes, Christopher S. Wu, Kris Honraet, Dorien Staljanssens, Bart Rossel, J Marshall Clark

SIUE Faculty Research, Scholarship, and Creative Activity

The human head louse is a cosmopolitan ectoparasite and frequently infests many people, particularly school-age children. Due to widespread pyrethroid resistance and the lack of efficient resistance management, there has been a considerable interest in the protection of uninfested people and prevention of reinfestation by disrupting lice transfer. In this study, two nonclinical model systems (in vitro and in vivo) were used to determine the efficacy of the infestation deterrents, Elimax lotion and Elimax shampoo, against human head lice or poultry chewing lice, respectively. With in vitro assessments, female head lice exhibited significantly higher avoidance responses to hair tufts treated ...


Quantitative Real-Time Polymerase Chain Reaction For Detecting Mycoplasma Hyosynoviae And Mycoplasma Hyorhinis In Pen-Based Oral, Tonsillar, And Nasal Fluids, Joao C. Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 2015 Iowa State University

Quantitative Real-Time Polymerase Chain Reaction For Detecting Mycoplasma Hyosynoviae And Mycoplasma Hyorhinis In Pen-Based Oral, Tonsillar, And Nasal Fluids, Joao C. Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait

Veterinary Diagnostic and Production Animal Medicine Publications

Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd surveillance. Two methods of genomic DNA extraction, automated versus manual, were used to compare diagnostic test performance. A wean-to-finish longitudinal study was also carried out to demonstrate ...


Survey Of Serum Vitamin D Status Across Stages Of Swine Production And Evaluation Of Supplemental Bulk Vitamin D Premixes Used In Swine Diets, Jessica Arnold, Darin M. Madson, Steve Ensley, Jesse P. Goff, Chris Sparks, Gregory W. Stevenson, Thomas Crenshaw, Chong Wang, Ronald L. Horst 2015 Iowa State University

Survey Of Serum Vitamin D Status Across Stages Of Swine Production And Evaluation Of Supplemental Bulk Vitamin D Premixes Used In Swine Diets, Jessica Arnold, Darin M. Madson, Steve Ensley, Jesse P. Goff, Chris Sparks, Gregory W. Stevenson, Thomas Crenshaw, Chong Wang, Ronald L. Horst

Veterinary Diagnostic and Production Animal Medicine Publications

The objectives of this investigation were to evaluate the serum 25-hydroxyvitamin D [25(OH)D] concentrations in pigs of different age groups, to determine if 25(OH)D concentrations varied with season, and to assess the quality of vitamin D supplements used in swine diets from multiple commercial suppliers. Serum samples (n = 1200) submitted to a diagnostic laboratory for routine surveillance were assayed for serum 25(OH)D concentrations. Vitamin D premix samples were obtained from suppliers and analyzed at two laboratories over a 9-month period. In all age categories, 25(OH)D concentrations in numerous serum samples were lower ...


Uterine Artery Rupture, An Angiopathy Of The Reproductive System Of The Mare: Occurrence And Potential Effects, Ana G. Toro Mayorga 2015 University of Kentucky

Uterine Artery Rupture, An Angiopathy Of The Reproductive System Of The Mare: Occurrence And Potential Effects, Ana G. Toro Mayorga

Theses and Dissertations--Veterinary Science

The intent of this research was to identify if the degenerative changes within arteries in the endometrium (endometrial angiopathies) correlate with degenerative changes in the uterine arteries and can be used as a predictor of increased risk for uterine artery rupture (UAR). With this objective specimens from 20 mares that died from uterine artery rupture and 21 control mares that died from unrelated causes were obtained from cases submitted to the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) over a two-year period. Postmortem specimens of each mare were collected from the left and right uterine arteries at the origin, bifurcation ...


Widespread Detection Of Antibodies To Leptospira In Feral Swine In The United States, K. Pedersen, K. L. Pabilonia, T. D. Anderson, S. N. Bevins, C. R. Hicks, J. M. Kloft, Thomas J. Deliberto 2015 U.S. Department of Agriculture, Animal and Plant Health Inspection Service

Widespread Detection Of Antibodies To Leptospira In Feral Swine In The United States, K. Pedersen, K. L. Pabilonia, T. D. Anderson, S. N. Bevins, C. R. Hicks, J. M. Kloft, Thomas J. Deliberto

USDA National Wildlife Research Center - Staff Publications

As feral swine continue to expand their geographical range and distribution across the United States, their involvement in crop damage, livestock predation, and pathogen transmission is likely to increase. Despite the relatively recent discovery of feral swine involvement in the aetiology of a variety of pathogens, their propensity to transmit and carry a wide variety of pathogens is disconcerting. We examined sera from 2055 feral swine for antibody presence to six serovars of Leptospira that can also infect humans, livestock or domestic animals. About 13% of all samples tested positive for at least one serovar, suggesting that Leptospira infection is ...


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