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Pathogenicity Of An Emergent, Ovine Abortifacient Campylobacter Jejuni Clone Orally Inoculated Into Pregnant Guinea Pigs, Eric R. Burrough, Orhan Sahin, Paul J. Plummer, Qijing Zhang, Michael J. Yaeger 2017 Iowa State University

Pathogenicity Of An Emergent, Ovine Abortifacient Campylobacter Jejuni Clone Orally Inoculated Into Pregnant Guinea Pigs, Eric R. Burrough, Orhan Sahin, Paul J. Plummer, Qijing Zhang, Michael J. Yaeger

Paul Plummer

Objective—To compare pathogenicity of an emergent abortifacient Campylobacter jejuni (IA 3902) with that of reference strains after oral inoculation in pregnant guinea pigs. Animals—58 pregnant guinea pigs. Procedures—12 animals were challenged IP with C jejuni IA 3902 along with 5 sham-inoculated control animals to confirm abortifacient potential. Once pathogenicity was confirmed, challenge via oral inoculation was performed whereby 12 guinea pigs received IA 3902, 12 received C jejuni isolated from ovine feces (OF48), 12 received a fully sequenced human C jejuni isolate (NCTC 11168), and 5 were sham-inoculated control animals. After abortions, guinea pigs were euthanized; samples ...


Comparison Of Two Commercial Ovine Campylobacter Vaccines And An Experimental Bacterin In Guinea Pigs Inoculated With Campylobacter Jejuni, Eric R. Burrough, Orhan Sahin, Paul J. Plummer, Kevin D. DiVerde, Qijing Zhang, Michael J. Yaeger 2017 Iowa State University

Comparison Of Two Commercial Ovine Campylobacter Vaccines And An Experimental Bacterin In Guinea Pigs Inoculated With Campylobacter Jejuni, Eric R. Burrough, Orhan Sahin, Paul J. Plummer, Kevin D. Diverde, Qijing Zhang, Michael J. Yaeger

Paul Plummer

Objective—To compare efficacy of 2 commercial ovine Campylobacter vaccines and an experimental bacterin in guinea pigs following IP inoculation with Campylobacter jejuni IA3902. Animals—51 female guinea pigs. Procedures—Pregnant and nonpregnant animals were randomly assigned to 1 of 4 treatment groups and administered a commercial Campylobacter vaccine labeled for prevention of campylobacteriosis in sheep via two 5-mL doses 14 days apart (vaccine A; n = 13), another labeled for prevention of campylobacteriosis via two 2-mL doses (vaccine B; 12), an experimental bacterin prepared from the challenge strain (12), or a sham vaccine (14). Ten days later, animals were challenged ...


Effect Of Macrolide Usage On Emergence Of Erythromycin-Resistant Campylobacter Isolates In Chickens, Jun Lin, Meiguan Yan, Orhan Sahin, Sonia Pereira, Yun-Juan Chang, Qijing Zhang, Qijing Zhang 2017 University of Tennessee, Knoxville

Effect Of Macrolide Usage On Emergence Of Erythromycin-Resistant Campylobacter Isolates In Chickens, Jun Lin, Meiguan Yan, Orhan Sahin, Sonia Pereira, Yun-Juan Chang, Qijing Zhang, Qijing Zhang

Qijing Zhang

In this work we conducted both in vitro and in vivo experiments to examine the development and mechanisms of erythromycin (Ery) resistance in Campylobacter jejuni and Campylobacter coli. In vitro plating revealed that both Campylobacter species had similar but low spontaneous mutation frequencies (3 × 10−9 to −10) for Ery resistance. Chickens infected with C. jejuni or C. coli were subjected to single or multiple treatments with medicated water containing tylosin (0.53 g/liter), which transiently reduced the level of Campylobacter colonization but did not select for Ery-resistant (Eryr) mutants in the treated birds. However, when tylosin was given ...


The Campylobacter Jejuni Response Regulator, Cbrr, Modulates Sodium Deoxycholate Resistance And Chicken Colonization, Brian H. Raphael, Sonia Pereira, Gary A. Flom, Qijing Zhang, Julian M. Ketley, Michael E. Konkel 2017 Washington State University

The Campylobacter Jejuni Response Regulator, Cbrr, Modulates Sodium Deoxycholate Resistance And Chicken Colonization, Brian H. Raphael, Sonia Pereira, Gary A. Flom, Qijing Zhang, Julian M. Ketley, Michael E. Konkel

Qijing Zhang

Two-component regulatory systems play a major role in the physiological response of bacteria to environmental stimuli. Such systems are composed of a sensor histidine kinase and a response regulator whose ultimate function is to affect the expression of target genes. Response regulator mutants of Campylobacter jejuni strain F38011 were screened for sensitivity to sodium deoxycholate. A mutation in Cj0643, which encodes a response regulator with no obvious cognate histidine kinase, resulted in an absence of growth on plates containing a subinhibitory concentration of sodium deoxcholate (1%, wt/vol). In broth cultures containing 0.05% (wt/vol) sodium deoxycholate, growth of ...


Salicylate Functions As An Efflux Pump Inducer And Promotes The Emergence Of Fluoroquinolone-Resistant Campylobacter Jejuni Mutants, Zhangqi Shen, Xiao-Ying Pu, Qijing Zhang 2017 Iowa State University

Salicylate Functions As An Efflux Pump Inducer And Promotes The Emergence Of Fluoroquinolone-Resistant Campylobacter Jejuni Mutants, Zhangqi Shen, Xiao-Ying Pu, Qijing Zhang

Qijing Zhang

Salicylate, a nonsteroidal anti-inflammatory compound, has been shown to increase the resistance of Campylobacter to antimicrobials. However, the molecular mechanism underlying salicylate-induced resistance has not yet been established. In this study, we determined how salicylate increases antibiotic resistance and evaluated its impact on the development of fluoroquinolone-resistant Campylobacter mutants. Transcriptional fusion assays, real-time quantitative reverse transcription-PCR (RT-PCR), and immunoblotting assays consistently demonstrated the induction of the CmeABC multidrug efflux pump by salicylate. Electrophoretic mobility shift assays further showed that salicylate inhibits the binding of CmeR (a transcriptional repressor of the TetR family) to the promoter DNA of cmeABC, suggesting that ...


Identification Of An Arsenic Resistance And Arsenic-Sensing System In Campylobacter Jejuni, Liping Wang, Byeonghwa Jeon, Orhan Sahin, Qijing Zhang 2017 Iowa State University

Identification Of An Arsenic Resistance And Arsenic-Sensing System In Campylobacter Jejuni, Liping Wang, Byeonghwa Jeon, Orhan Sahin, Qijing Zhang

Qijing Zhang

Arsenic is commonly present in the natural environment and is also used as a feed additive for animal production. Poultry is a major reservoir for Campylobacter jejuni, a major food-borne human pathogen causing gastroenteritis. It has been shown that Campylobacter isolates from poultry are highly resistant to arsenic compounds, but the molecular mechanisms responsible for the resistance have not been determined, and it is unclear if the acquired arsenic resistance affects the susceptibility of Campylobacter spp. to other antimicrobials. In this study, we identified a four-gene operon that contributes to arsenic resistance in Campylobacter. This operon encodes a putative membrane ...


Workshop Report: The 2012 Antimicrobial Agents In Veterinary Medicine: Exploring The Consequences Of Antimicrobial Drug Use: A 3-D Approach, M. Martinez, J. Blondeau, C. E. Cernigilia, J. Fink-Gremmels, S. Guenther, R. P. Hunter, X.-Z. Li, M. Papich, P. Silley, S. Soback, P.-L. Toutain, Q. Zhang 2017 U.S. Food and Drug Administration

Workshop Report: The 2012 Antimicrobial Agents In Veterinary Medicine: Exploring The Consequences Of Antimicrobial Drug Use: A 3-D Approach, M. Martinez, J. Blondeau, C. E. Cernigilia, J. Fink-Gremmels, S. Guenther, R. P. Hunter, X.-Z. Li, M. Papich, P. Silley, S. Soback, P.-L. Toutain, Q. Zhang

Qijing Zhang

Antimicrobial resistance is a global challenge that impacts both human and veterinary health care. The resilience of microbes is reflected in their ability to adapt and survive in spite of our best efforts to constrain their infectious capabilities. As science advances, many of the mechanisms for microbial survival and resistance element transfer have been identified. During the 2012 meeting of Antimicrobial Agents in Veterinary Medicine (AAVM), experts provided insights on such issues as use vs. resistance, the available tools for supporting appropriate drug use, the importance of meeting the therapeutic needs within the domestic animal health care, and the requirements ...


Role Of Cj1211 In Natural Transformation And Transfer Of Antibiotic Resistance Determinants In Campylobacter Jejuni, Byeonghwa Jeon, Wayne Muraoka, Orhan Sahin, Qijing Zhang 2017 Iowa State University

Role Of Cj1211 In Natural Transformation And Transfer Of Antibiotic Resistance Determinants In Campylobacter Jejuni, Byeonghwa Jeon, Wayne Muraoka, Orhan Sahin, Qijing Zhang

Qijing Zhang

Campylobacter jejuni, an important food-borne human pathogen, is increasingly resistant to antimicrobials. Natural transformation is considered to be a main mechanism for mediating the transfer of genetic materials encoding antibiotic resistance determinants in C. jejuni, but direct evidence for this notion is still lacking. In this study, we determined the role of Cj1211 in natural transformation and in the development of antibiotic resistance in C. jejuni. Insertional mutagenesis of Cj1211, a Helicobacter pylori ComH3 homolog, abolished natural transformation in C. jejuni. In vitro coculture of C. jejuni strains carrying either kanamycin or tetracycline resistance markers demonstrated the development of progenies ...


Bile Salts Modulate Expression Of The Cmeabc Multidrug Efflux Pump In Campylobacter Jejuni, Jun Lin, Cédric Cagliero, Baoqing Guo, Yi-Wen Barton, Marie-Christine Maurel, Sophie Payot, Qijing Zhang 2017 Iowa State University

Bile Salts Modulate Expression Of The Cmeabc Multidrug Efflux Pump In Campylobacter Jejuni, Jun Lin, Cédric Cagliero, Baoqing Guo, Yi-Wen Barton, Marie-Christine Maurel, Sophie Payot, Qijing Zhang

Qijing Zhang

CmeABC, a multidrug efflux pump, is involved in the resistance of Campylobacter jejuni to a broad spectrum of antimicrobial agents and is essential for Campylobacter colonization in animal intestine by mediating bile resistance. Previously, we have shown that expression of this efflux pump is under the control of a transcriptional repressor named CmeR. Inactivation of CmeR or mutation in the cmeABC promoter (PcmeABC) region derepresses cmeABC, leading to overexpression of this efflux pump. However, it is unknown if the expression of cmeABC can be conditionally induced by the substrates it extrudes. In this study, we examined the expression of ...


Cmer Functions As A Transcriptional Repressor For The Multidrug Efflux Pump Cmeabc In Campylobacter Jejuni, Jun Lin, Masato Akiba, Orhan Sahin, Qijing Zhang 2017 Iowa State University

Cmer Functions As A Transcriptional Repressor For The Multidrug Efflux Pump Cmeabc In Campylobacter Jejuni, Jun Lin, Masato Akiba, Orhan Sahin, Qijing Zhang

Qijing Zhang

CmeABC, a resistance-nodulation-division (RND) type of efflux pump, contributes to Campylobacter resistance to a broad spectrum of antimicrobial agents and is also essential for Campylobacter colonization of the animal intestinal tract by mediation of bile resistance. As one of the main systems for Campylobacter adaptation to different environments, CmeABC is likely subject to control by regulatory elements. We describe the identification of a transcriptional repressor for CmeABC. Insertional mutagenesis of cmeR, an open reading frame immediately upstream of the cmeABC operon, resulted in overexpression of cmeABC, as determined by transcriptional fusion (PcmeABC-lacZ) and immunoblotting with CmeABC-specific antibodies. Overexpression of the ...


Distribution Of The Multidrug Resistance Gene Cfr In Staphylococcus Species Isolates From Swine Farms In China, Yang Wang, Wanjiang Zhang, Juan Wang, Congming Wu, Zhangqi Shen, Xiao Fu, Yang Yan, Qijing Zhang, Stefan Schwarz, Jianzhong Shen 2017 China Agricultural University

Distribution Of The Multidrug Resistance Gene Cfr In Staphylococcus Species Isolates From Swine Farms In China, Yang Wang, Wanjiang Zhang, Juan Wang, Congming Wu, Zhangqi Shen, Xiao Fu, Yang Yan, Qijing Zhang, Stefan Schwarz, Jianzhong Shen

Qijing Zhang

A total of 149 porcine Staphylococcus isolates with florfenicol MICs of ≥16 μg/ml were screened for the presence of the multiresistance gene cfr, its location on plasmids, and its genetic environment. In total, 125 isolates carried either cfr (16 isolates), fexA (92 isolates), or both genes (17 isolates). The 33 cfr-carrying staphylococci, which included isolates of the species Staphylococcus cohnii, S. arlettae, and S. saprophyticus in which the cfr gene has not been described before, exhibited a wide variety of SmaI pulsed-field gel electrophoresis patterns. In 18 cases, the cfr gene was located on plasmids. Four different types ...


Impaired Fitness And Transmission Of Macrolide-Resistant Campylobacter Jejuni In Its Natural Host, Taradon Luangtongkum, Zhangqi Shen, Virginia W. Seng, Orhan Sahin, Byeonghwa Jeon, Peng Liu, Qijing Zhang 2017 Iowa State University

Impaired Fitness And Transmission Of Macrolide-Resistant Campylobacter Jejuni In Its Natural Host, Taradon Luangtongkum, Zhangqi Shen, Virginia W. Seng, Orhan Sahin, Byeonghwa Jeon, Peng Liu, Qijing Zhang

Qijing Zhang

Campylobacter jejuni is a major zoonotic pathogen transmitted to humans via the food chain and is prevalent in chickens, a natural reservoir for this pathogenic organism. Due to the importance of macrolide antibiotics in clinical therapy of human campylobacteriosis, development of macrolide resistance in Campylobacter has become a concern for public health. To facilitate the control of macrolide-resistant Campylobacter, it is necessary to understand if macrolide resistance affects the fitness and transmission of Campylobacter in its natural host. In this study we conducted pairwise competitions and comingling experiments in chickens using clonally related and isogenic C. jejuni strains, which are ...


Cj0011c, A Periplasmic Single- And Double-Stranded Dna-Binding Protein, Contributes To Natural Transformation In Campylobacter Jejuni, Byeonghwa Jeon, Qijing Zhang 2017 Iowa State University

Cj0011c, A Periplasmic Single- And Double-Stranded Dna-Binding Protein, Contributes To Natural Transformation In Campylobacter Jejuni, Byeonghwa Jeon, Qijing Zhang

Qijing Zhang

Campylobacter jejuni is an important bacterial pathogen causing gastroenteritis in humans. C. jejuni is capable of natural transformation, which is considered a major mechanism mediating horizontal gene transfer and generating genetic diversity. Despite recent efforts to elucidate the transformation mechanisms of C. jejuni, the process of DNA binding and uptake in this organism is still not well understood. In this study, we report a previously unrecognized DNA-binding protein (Cj0011c) in C. jejuni that contributes to natural transformation. Cj0011c is a small protein (79 amino acids) with a partial sequence homology to the C-terminal region of ComEA in Bacillus subtilis. Cj0011c ...


Comparison Of Antimicrobial Susceptibility Testing Of Campylobacter Spp. By The Agar Dilution And The Agar Disk Diffusion Methods, Taradon Luangtongkum, Teresa Y. Morishita, Amna B. El-Tayeb, Aaron J. Ison, Qijing Zhang 2017 The Ohio State University

Comparison Of Antimicrobial Susceptibility Testing Of Campylobacter Spp. By The Agar Dilution And The Agar Disk Diffusion Methods, Taradon Luangtongkum, Teresa Y. Morishita, Amna B. El-Tayeb, Aaron J. Ison, Qijing Zhang

Qijing Zhang

The correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of Campylobacter were investigated. A high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics.


Prevalence Of Tetracycline-Resistant Campylobacter In Organic Broilers During A Production Cycle, Taradon Luangtongkum, Teresa Y. Morishita, Lori Martin, Irene Choi, Orhan Sahin, Qijing Zhang 2017 The Ohio State University

Prevalence Of Tetracycline-Resistant Campylobacter In Organic Broilers During A Production Cycle, Taradon Luangtongkum, Teresa Y. Morishita, Lori Martin, Irene Choi, Orhan Sahin, Qijing Zhang

Qijing Zhang

Tetracycline (tet) resistance in Campylobacter isolated from organically raised broilers was investigated in this study. Two hundred forty-five samples from an organic broiler farm were collected weekly from the first week to the end of the production cycle, and they were cultured for thermophilic Campylobacter. Tetracycline resistance of these Campylobacter isolates was identified by the agar dilution method, whereas DNA fingerprinting profiles of tet-susceptible and tet-resistant strains were determined by pulsed-field gel electrophoresis (PFGE). None of the Campylobacter isolates from the third and the fourth week of the production period were resistant to tetracycline, whereas 66.7% of the isolates ...


Roles Of Lipooligosaccharide And Capsular Polysaccharide In Antimicrobial Resistance And Natural Transformation Of Campylobacter Jejuni, Byeonghwa Jeon, Wayne Muraoka, Alexandra Scupham, Qijing Zhang 2017 Iowa State University

Roles Of Lipooligosaccharide And Capsular Polysaccharide In Antimicrobial Resistance And Natural Transformation Of Campylobacter Jejuni, Byeonghwa Jeon, Wayne Muraoka, Alexandra Scupham, Qijing Zhang

Qijing Zhang

Objectives

This study is aimed to determine the role of capsular polysaccharide (CPS) and lipooligosaccharide (LOS) in modulating antimicrobial resistance and natural transformation of Campylobacter jejuni, an important food-borne human pathogen.

Methods

A series of C. jejuni mutants, which are defective in either CPS or LOS or both, were constructed. The antimicrobial susceptibility, bacterial surface hydrophobicity, natural transformation frequency and DNA binding and uptake were measured and compared between the mutants and the wild-type strain.

Results

Truncation of LOS greatly reduced (8-fold) the intrinsic resistance of C. jejuni to erythromycin, a key antibiotic used for treating human campylobacteriosis, while the ...


Transcriptional Regulation Of The Cmeabc Multidrug Efflux Pump And The Kata Catalase By Cosr In Campylobacter Jejuni, Sunyoung Hwang, Qijing Zhang, Sangryeol Ryu, Byeonghwa Jeon 2017 Seoul National University

Transcriptional Regulation Of The Cmeabc Multidrug Efflux Pump And The Kata Catalase By Cosr In Campylobacter Jejuni, Sunyoung Hwang, Qijing Zhang, Sangryeol Ryu, Byeonghwa Jeon

Qijing Zhang

CosR is an essential response regulator in Campylobacter jejuni, a major food-borne pathogen causing enteritis worldwide. A transcriptomic analysis performed in this study discovered 93 genes whose transcriptional levels were changed >2-fold due to the repression of CosR expression by antisense peptide nucleic acid. The identified CosR-regulated genes are involved in various cellular functions, such as energy production, protein synthesis and folding, flagellum biogenesis, and lipid metabolism. Interestingly, 17 of the 93 CosR-regulated genes (18.3%) are predicted essential genes, indicating that CosR may participate in the regulation of vital biological processes in C. jejuni. In particular, CosR knockdown increased ...


The New Genetic Environment Of Cfr On Plasmid Pbs-02 In A Bacillus Strain, Wan-Jiang Zhang, Cong-Ming Wu, Yang Wang, Zhang-Qi Shen, Lei Dai, Jing Han, Steven L. Foley, Jian-Zhong Shen, Qijing Zhang 2017 China Agricultural University

The New Genetic Environment Of Cfr On Plasmid Pbs-02 In A Bacillus Strain, Wan-Jiang Zhang, Cong-Ming Wu, Yang Wang, Zhang-Qi Shen, Lei Dai, Jing Han, Steven L. Foley, Jian-Zhong Shen, Qijing Zhang

Qijing Zhang

Sir,

The gene cfr, encoding a 23S rRNA methyltransferase, confers resistance to five chemically unrelated antimicrobial classes, including phenicols, lincosamides, oxazolidinones, pleuromutilins and streptogramin A (PhLOPSA), and has been observed mainly in staphylococcal isolates over the past decade.1–3 Our previous study reported a cfr-carrying plasmid, pBS-01, in a novel strain (BS-01) of a Bacillus species isolated from a pig farm in China.4 In our routine surveillance study on antimicrobial resistance in farm animals in 2010, another florfenicol and chloramphenicol-resistant Bacillus species strain (named BS-02) from swine faeces was identified. Gram staining, sequence analysis of the ...


Synergistic Effects Of Anti-Cmea And Anti-Cmeb Peptide Nucleic Acids On Sensitizing Campylobacter Jejuni To Antibiotics, Yang Mu, Zhangqi Shen, Byeonghwa Jeon, Lei Dai, Qijing Zhang 2017 Iowa State University

Synergistic Effects Of Anti-Cmea And Anti-Cmeb Peptide Nucleic Acids On Sensitizing Campylobacter Jejuni To Antibiotics, Yang Mu, Zhangqi Shen, Byeonghwa Jeon, Lei Dai, Qijing Zhang

Qijing Zhang

The CmeABC efflux pump in Campylobacter jejuni confers resistance to structurally divergent antimicrobials, and inhibition of CmeABC represents a promising strategy to control antibiotic-resistant Campylobacter. Antisense peptide nucleic acids (PNAs) targeting the three components of CmeABC were evaluated for inhibition of CmeABC expression. The result revealed a synergistic effect of the PNAs targeting CmeA and CmeB on sensitizing C. jejuni to antibiotics. This finding further demonstrates the feasibility of using PNAs to potentiate antibiotics against antibiotic-resistant Campylobacter.


Target Optimization For Peptide Nucleic Acid (Pna)-Mediated Antisense Inhibition Of The Cmeabc Multidrug Efflux Pump In Campylobacter Jejuni, Euna Oh, Qijing Zhang, Byeonghwa Jeon 2017 University of Alberta

Target Optimization For Peptide Nucleic Acid (Pna)-Mediated Antisense Inhibition Of The Cmeabc Multidrug Efflux Pump In Campylobacter Jejuni, Euna Oh, Qijing Zhang, Byeonghwa Jeon

Qijing Zhang

Objectives CmeABC is a resistance-nodulation-cell division (RND)-type multidrug efflux pump conferring resistance to clinically important antibiotics in Campylobacter. This study aimed to identify the optimal target sites for the inhibition of CmeABC with antisense peptide nucleic acid (PNA). Methods Eighteen PNAs were designed to bind to the translational initiation regions of cmeABC, spanning the ribosome-binding site (RBS) and the start codon of the cmeABC genes. Campylobacter jejuni was treated with CmeABC-specific PNAs (CmeABC-PNAs) at various concentrations and subjected to western blotting to measure changes in the level of CmeABC expression. The MICs of ciprofloxacin and erythromycin were measured to ...


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