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2004 Full-Text Articles 3094 Authors 143650 Downloads 21 Institutions

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2004 full-text articles. Page 1 of 30.

Generating An Equation To Predict Post- Farrow Maternal Weight In Multiple Parity Sows, L. L. Thomas, S. S. Dritz, R. D. Goodband, M. D. Tokach, J. M. DeRouchey, J. C. Woodworth 2016 Kansas State University, Manhattan

Generating An Equation To Predict Post- Farrow Maternal Weight In Multiple Parity Sows, L. L. Thomas, S. S. Dritz, R. D. Goodband, M. D. Tokach, J. M. Derouchey, J. C. Woodworth

Kansas Agricultural Experiment Station Research Reports

ost-farrow maternal weight is required when partitioning maternal and fetal weight gains throughout gestation. Equations were developed from the analysis of 150 females (Line 1050, PIC, Hendersonville, TN) to predict the weight of conceptus by difference of pre- and post-farrowing weight change in multi-parity sows. Females were individually weighed as they were moved into the farrowing house at d 110 to 112 of gestation and again at 12 to 24 h after farrowing. Data were divided into 4 groups: (1) parity 1 sows; (2) parity 2 sows; (3) parity 3 sows; and (4) parity 4+ sows. Each group tested 3 ...


Evaluation Of The Effects Of Flushing Feed Manufacturing Equipment With Chemically- Treated Rice Hulls On Porcine Epidemic Diarrhea Virus Cross Contamination During Feed Manufacturing, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, Phillip Charles Gauger, M. D. Tokach, J. M. DeRouchey, R. D. Goodband, M. Muckey, R. A. Cochrane, M. Niederwerder, C. R. Stark, J. Bai, Qi Chen, Jianqiang Zhang, Alejandro Ramirez, Rachel J. Derscheid, Rodger G. Main, S. S. Dritz 2016 Kansas State University, Manhattan

Evaluation Of The Effects Of Flushing Feed Manufacturing Equipment With Chemically- Treated Rice Hulls On Porcine Epidemic Diarrhea Virus Cross Contamination During Feed Manufacturing, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, Phillip Charles Gauger, M. D. Tokach, J. M. Derouchey, R. D. Goodband, M. Muckey, R. A. Cochrane, M. Niederwerder, C. R. Stark, J. Bai, Qi Chen, Jianqiang Zhang, Alejandro Ramirez, Rachel J. Derscheid, Rodger G. Main, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

Various strategies have been proposed to mitigate potential risk of porcine epidemic diarrhea virus (PEDV) transmission via feed and feed ingredients. Wet decontamination has been found to be the most effective decontamination of feed mill surfaces; however, this is not practical on a commercial feed production-scale. Another potential mitigation strategy, easier to implement, would be using chemically-treated rice hulls flushed through the feed manufacturing equipment. The objective of this experiment was to determine the impact of MCFA- or formaldehyde-treated rice hull flush batches as potential PEDV mitigation strategies during feed manufacturing. Feed without evidence of PEDV RNA contamination was inoculated ...


Evaluating The Impact Of Vevovitall And/Or Crina As Potential Porcine Epidemic Diarrhea Virus Mitigation Strategies As Determined By Polymerase Chain Reaction Analysis And Bioassay, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, M. D. Tokach, J. M. DeRouchey, R. D. Goodband, R. A. Cochrane, C. R. Stark, J. Bergstrom, Phillip Charles Gauger, J. Bai, Qi Chen, Jianqiang Zhang, Rodger G. Main, S. S. Dritz 2016 Kansas State University, Manhattan

Evaluating The Impact Of Vevovitall And/Or Crina As Potential Porcine Epidemic Diarrhea Virus Mitigation Strategies As Determined By Polymerase Chain Reaction Analysis And Bioassay, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, M. D. Tokach, J. M. Derouchey, R. D. Goodband, R. A. Cochrane, C. R. Stark, J. Bergstrom, Phillip Charles Gauger, J. Bai, Qi Chen, Jianqiang Zhang, Rodger G. Main, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

Feed and feed ingredients have been shown to be potential vectors of porcine epidemic diarrhea virus (PEDV). Potential strategies to mitigate the risk of disease transmission via feed and feed ingredients would be valuable to the swine and feed milling industries. Therefore, the objective of this experiment was to determine the impact of VevoVitall (5,000 ppm; DSM Nutritional Products Inc., Parsipanny, NJ), CRINA (200 ppm; DSM Nutritional Products Inc., Parsipanny, NJ), and a combination of both products (COMBINATION; 5,000 ppm VevoVitall and 200 ppm CRINA) as feed additives with potential to mitigate the risk of PEDV, in swine ...


Porcine Epidemic Diarrhea Virus Surface Decontamination Strategies Using Chemical Sanitizing To Reduce The Quantity Of Pedv Rna On Feed Manufacturing Surfaces With Environmental Swabbing, M. Muckey, S. S. Dritz, J. C. Woodworth, C. R. Stark, J. Bai, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones 2016 Kansas State University, Manhattan

Porcine Epidemic Diarrhea Virus Surface Decontamination Strategies Using Chemical Sanitizing To Reduce The Quantity Of Pedv Rna On Feed Manufacturing Surfaces With Environmental Swabbing, M. Muckey, S. S. Dritz, J. C. Woodworth, C. R. Stark, J. Bai, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Porcine Epidemic Diarrhea virus (PEDV) is a possible hazard in feed mills that could impact pig health. If the virus enters a feed mill, it quickly becomes widely distributed and is difficult to decontaminate from surfaces.6,7 The objective of this study was to evaluate a variety of liquid and dry chemical treatments that could be used as sanitizers to reduce the amount of PEDV found on feed manufacturing surfaces in mills. This experiment was replicated 3 times and was designed in a 5 × 10 factorial with main effects of 5 different feed manufacturing surfaces and 10 sanitizing treatments ...


Evaluating The Inclusion Level Of Medium Chain Fatty Acids To Reduce The Risk Of Porcine Epidemic Diarrhea Virus In Complete Feed And Spray-Dried Animal Plasma, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. DeRouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Drew Robert Magstadt, Paulo Elias Arruda, Alejandro Ramirez, Rodger G. Main, C. K. Jones 2016 Kansas State University, Manhattan

Evaluating The Inclusion Level Of Medium Chain Fatty Acids To Reduce The Risk Of Porcine Epidemic Diarrhea Virus In Complete Feed And Spray-Dried Animal Plasma, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Drew Robert Magstadt, Paulo Elias Arruda, Alejandro Ramirez, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of MCFA levels below 2% inclusion is unknown. The objective of this experiment was to evaluate if a 1% inclusion of MCFA is as effective at PEDV mitigation as a 2% inclusion or formaldehyde in swine feed and spray-dried animal plasma (SDAP). Treatments were arranged in a 4 × 2 × 7 plus 2 factorial with 4 chemical treatments: 1) PEDV positive with no chemical treatment, 2 ...


Assessing The Effects Of Medium Chain Fatty Acids And Fat Sources On Porcine Epidemic Diarrhea Virus Viral Rna Stability And Infectivity, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. DeRouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Rodger G. Main, C. K. Jones 2016 Kansas State University, Manhattan

Assessing The Effects Of Medium Chain Fatty Acids And Fat Sources On Porcine Epidemic Diarrhea Virus Viral Rna Stability And Infectivity, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of individual MCFA levels are unknown. The objective of this study is to compare the efficacy of commercially-available sources of MCFA and other fat sources versus a synthetic custom blend of MCFA to minimize the risk of PEDV cross-contamination as measured by qRT-PCR and bioassay. Treatments were arranged in a 17 × 4 plus 1 factorial with 17 chemical treatments: 1) Positive control with PEDV and ...


Kinetics Of Uv254 Inactivation Of Selected Viral Pathogens In A Static System, T. Cutler, C. Wang, Q. Qin, F. Zhou, K. Warren, K.-J. Yoon, S. J. Hoff, J. Ridpath, J. Zimmerman 2016 Iowa State University

Kinetics Of Uv254 Inactivation Of Selected Viral Pathogens In A Static System, T. Cutler, C. Wang, Q. Qin, F. Zhou, K. Warren, K.-J. Yoon, S. J. Hoff, J. Ridpath, J. Zimmerman

Steven J. Hoff

Aims:  The objective of this study was to estimate UV254 inactivation constants for four viral pathogens: influenza virus type A, porcine respiratory and reproductive syndrome virus (PRRSV), bovine viral diarrhoea virus (BVDV) and reovirus.

Methods and Results:  Viruses in culture medium were exposed to one of nine doses of UV254 and then titrated for infectious virus. Analysis showed that viral inactivation by UV254 was more accurately described by a two-stage inactivation model vs a standard one-stage inactivation model.

Conclusions:  The results provided evidence for the existence of two heterogeneous viral subpopulations among the viruses tested, one highly ...


Evaluation Of The Cross-Protective Efficacy Of A Chimeric Porcine Reproductive And Respiratory Syndrome Virus Constructed Based On Two Field Strains, Nadeem Shabir, Amina Khatun, Salik Nazki, Bumseok Kim, Eun-Jin Choi, Dong Sun, Kyoung-Jin Yoon, Won-Il Kim 2016 Chonbuk National University

Evaluation Of The Cross-Protective Efficacy Of A Chimeric Porcine Reproductive And Respiratory Syndrome Virus Constructed Based On Two Field Strains, Nadeem Shabir, Amina Khatun, Salik Nazki, Bumseok Kim, Eun-Jin Choi, Dong Sun, Kyoung-Jin Yoon, Won-Il Kim

Veterinary Diagnostic and Production Animal Medicine Publications

One of the major hurdles to porcine reproductive and respiratory syndrome (PRRS) vaccinology is the limited or no cross-protection conferred by current vaccines. To overcome this challenge, a PRRS chimeric virus (CV) was constructed using an FL12-based cDNA infectious clone in which open reading frames (ORFs) 3–4 and ORFs 5–6 were replaced with the two Korean field isolates K08-1054 and K07-2273,respectively. This virus was evaluated as a vaccine candidate to provide simultaneous protection against two genetically distinct PRRS virus (PRRSV) strains. Thirty PRRS-negative three-week-old pigs were divided into five groups and vaccinated with CV, K08-1054, K07-2273, VR-2332 ...


Impacts From The Use Of Antibiotics In Livestock: Methods Of Transmission Of Antibiotic Resistance From Livestock To Humans, Kristin M. Walden 2016 Augustana College, Rock Island Illinois

Impacts From The Use Of Antibiotics In Livestock: Methods Of Transmission Of Antibiotic Resistance From Livestock To Humans, Kristin M. Walden

Biology: Student Scholarship & Creative Works

Antibiotic use in livestock production has been around since the 1950s. Antibiotic feed is used in livestock and other meat producing animals for three reasons: illness prevention, illness treatment, and growth promotion. Unfortunately, since the time that antibiotics were first invented, antibiotic resistant bacteria have become a threat to public health. There are many studies showing methods of transmission of antibiotic resistance from livestock to humans. Antibiotic resistance can spread from livestock to soil, water, insects, and food, which ultimately comes into contact with humans. A proposed study to measure antibiotic resistance when eliminating antibiotic feed will provide a hypothesis ...


Studies On The Prevalence And Control Of Parasitic Helminths In "Natural" Laying Hens, Brittany R. Weir 2016 University of Arkansas, Fayetteville

Studies On The Prevalence And Control Of Parasitic Helminths In "Natural" Laying Hens, Brittany R. Weir

Animal Science Undergraduate Honors Theses

One societal trend that has been gaining much traction and popularity since the 21st century began is “organic” and/or “natural” food products. In 1999, the global market accounted for $15.2 billion dollars worth of organic food and drink, compared to the market in 2014 where we consumed $80 billion dollars worth (Willer et. al, 2016). With “natural” production of food animals however, “natural” parasite transmission may be a consequence. To that end, this experiment examines the prevalence of helminths in 110 “natural” laying hens from three regional farms and the efficacies of fenbendazole, piperazine, and levamisole on ...


Association Of Different Iowa Livestock Truck Wash Stations Service Levels With Enterobacteriaceae Counts, Amber Danielson, Samaneh Azarpajouh, Anna Johnson, James Dickson, Locke Karriker, Rodney Baker, Christopher Rademacher, Troy Bigelow, Kenneth Stalder 2016 Iowa State University

Association Of Different Iowa Livestock Truck Wash Stations Service Levels With Enterobacteriaceae Counts, Amber Danielson, Samaneh Azarpajouh, Anna Johnson, James Dickson, Locke Karriker, Rodney Baker, Christopher Rademacher, Troy Bigelow, Kenneth Stalder

Samaneh Azarpajouh

Data from eighteen different truck washes were used to compare the association of different service levels with Enterobacteriaceae counts. Service levels were classified into three different categories; prewash (n=78), post wash with disinfectant (n=78), and post wash without disinfectant (n=12). A total of 168 drag swabs were used for collection for the purpose of this study. Prewash services were defined as trailers before they were scraped out and washed. Post wash with or without disinfectant services were defined as after the trailers were washed and disinfectant was or was not applied. Prewash trailers tended to have higher ...


Therapeutic Neonatal Hepatic Gene Therapy In Mucopolysaccharidosis Vii Dogs, Katherine Parker Ponder, John Melniczek, Lingfei Xu, Margaret Weil, Thomas O'Malley, Patricia O'Donnell, Van Knox, Gustavo Aguirre, Hamutal Mazrier, N Ellinwood, Margaret Sleeper, Albert Maguire, Susan Volk, Robert Mango, Jean Zweigle, John Wolfe, Mark Haskins 2016 University of Pennsylvania

Therapeutic Neonatal Hepatic Gene Therapy In Mucopolysaccharidosis Vii Dogs, Katherine Parker Ponder, John Melniczek, Lingfei Xu, Margaret Weil, Thomas O'Malley, Patricia O'Donnell, Van Knox, Gustavo Aguirre, Hamutal Mazrier, N Ellinwood, Margaret Sleeper, Albert Maguire, Susan Volk, Robert Mango, Jean Zweigle, John Wolfe, Mark Haskins

Gustavo D. Aguirre, VMD, PhD

Dogs with mucopolysaccharidosis VII (MPS VII) were injected intravenously at 2–3 days of age with a retroviral vector (RV) expressing canine β-glucuronidase (cGUSB). Five animals received RV alone, and two dogs received hepatocyte growth factor (HGF) before RV in an attempt to increase transduction efficiency. Transduced hepatocytes expanded clonally during normal liver growth and secreted enzyme with mannose 6-phosphate. Serum GUSB activity was stable for up to 14 months at normal levels for the RV-treated dogs, and for 17 months at 67-fold normal for the HGF/RV-treated dog. GUSB activity in other organs was 1.5–60% of normal ...


Impact Of Carprofen Administration On Stress And Nociception Responses Of Calves To Cautery Dehorning, M. L. Stock, L. A. Barth, Nicholas K. Van Engen, Suzanne T. Millman, R. Gehring, Chong Wang, E. A. Voris, Larry W. Wulf, Lea Labeur, Walter H. Hsu, Johann F. Coetzee 2016 Iowa State University

Impact Of Carprofen Administration On Stress And Nociception Responses Of Calves To Cautery Dehorning, M. L. Stock, L. A. Barth, Nicholas K. Van Engen, Suzanne T. Millman, R. Gehring, Chong Wang, E. A. Voris, Larry W. Wulf, Lea Labeur, Walter H. Hsu, Johann F. Coetzee

Veterinary Diagnostic and Production Animal Medicine Publications

The objective of this study was to investigate the effects of carprofen administered immediately before cautery dehorning on nociception and stress. Forty Holstein calves aged approximately 6 to 8 wk old were either placebo treated and sham dehorned (n = 10) or cautery dehorned following administration of carprofen (1.4 mg/kg) subcutaneously (n = 10) or orally (n = 10) or a subcutaneous and oral placebo (n = 10) in a randomized, controlled trial. All animals were given a cornual nerve block using lidocaine before dehorning. Response variables including mechanical nociception threshold, ocular temperature, heart rate, and respiratory rate were measured before and ...


Cross-Sectional Analysis Of Salivary Cortisol And Cyathostome Infestation In Horses, Danielle L. Keating 2016 Otterbein University

Cross-Sectional Analysis Of Salivary Cortisol And Cyathostome Infestation In Horses, Danielle L. Keating

Honors Thesis Projects

With an increase in anthelmintic resistance and decreased efficacy of many commercial dewormers, understanding factors that contribute to parasite infestations in horses is integral to their management. The goal of this study was to look at the potential relationship between parasites and stress response by evaluating salivary cortisol levels and cyathostome egg shedding levels. Using a sample size of n = 200 horses from the state of Ohio, fecal and saliva samples were collected from each horse. Fecal egg counts were performed for each horse with a modified Stoll method, and saliva samples were tested with an enzyme-linked immunosorbent assay (ELISA ...


Table Of Contents 2016 Nebraska Beef Cattle Report, 2016 University of Nebraska - Lincoln

Table Of Contents 2016 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

No abstract provided.


A Survey Of Dairy Farm Treatment Practices On Midwest Dairy Farms, Patrick J. Gorden, Cassandra Rice, Adlai Schuler 2016 Iowa State University

A Survey Of Dairy Farm Treatment Practices On Midwest Dairy Farms, Patrick J. Gorden, Cassandra Rice, Adlai Schuler

Animal Industry Report

Judicious antimicrobial use and antimicrobial stewardship have become buzzwords in production animal agriculture over the last few years. While these words are becoming expectations in the industry, very little is understood about their true meaning and the level of implementation of judicious use practices on dairy farms. We conducted an investigation on 85 dairy farms in the Midwest to document drug use practices on these farms. Our results indicate that most farms are doing an adequate job of implementing judicious practices, but there is room for improvement to meet expectations of regulatory officials and consumers.


Altered Plasma Pharmacokinetics Of Ceftiofur Hydrochloride In Cows Affected With Severe Clinical Mastitis, Patrick J. Gorden, Michael D. Kleinhenz, Larry W. Wulf, Butch KuKanich, Chang J. Lee, Chong Wang, Johann F. Coetzee 2016 Iowa State University

Altered Plasma Pharmacokinetics Of Ceftiofur Hydrochloride In Cows Affected With Severe Clinical Mastitis, Patrick J. Gorden, Michael D. Kleinhenz, Larry W. Wulf, Butch Kukanich, Chang J. Lee, Chong Wang, Johann F. Coetzee

Animal Industry Report

Ceftiofur is the most commonly used antimicrobial in lactating dairy cows. Recently, there has been an increase in the number of violative residues of ceftiofur in the tissues of cull dairy cows. This was the first project in a series of projects we will be completing aimed at characterizing the pharmacokinetics of ceftiofur in disease challenged animals. The results of this study indicate that diseased animals have lower plasma concentrations and altered pharmacokinetics compared to healthy animals. Future work will investigate the influence of altered pharmacokinetics on the presence of violative residues.


In Vivo-Expressed Proteins Of Virulent Leptospira Interrogans Serovar Autumnalis N2 Elicit Strong Igm Responses Of Value In Conclusive Diagnosis, Veerapandian Raja, Santhanam Shanmughapriya, Murugesan Kanagavel, Sergey Artiushin, Sridhar Velineni, John F. Timoney, Kalimuthusamy Natarajaseenivasan 2016 Bharathidasan University, India

In Vivo-Expressed Proteins Of Virulent Leptospira Interrogans Serovar Autumnalis N2 Elicit Strong Igm Responses Of Value In Conclusive Diagnosis, Veerapandian Raja, Santhanam Shanmughapriya, Murugesan Kanagavel, Sergey Artiushin, Sridhar Velineni, John F. Timoney, Kalimuthusamy Natarajaseenivasan

Gluck Equine Research Center Faculty Publications

Leptospirosis is a serious zoonosis that is underdiagnosed because of limited access to laboratory facilities in Southeast Asia, Central and South America, and Oceania. Timely diagnosis of locally distributed serovars of high virulence is crucial for successful care and outbreak management. Using pooled patient sera, an expression gene library of a virulent Leptospira interrogans serovar Autumnalis strain N2 isolated in South India was screened. The identified genes were characterized, and the purified recombinant proteins were used as antigens in IgM enzyme-linked immunosorbent assay (ELISA) either singly or in combination. Sera (n = 118) from cases of acute leptospirosis along with sera ...


Iowa Beef Center, Dan Loy, Patrick J. Gunn, Beth E. Doran, Russ Euken, Denise L. Schwab, Chris Clark, Joe Sellers, Patrick B. Wall, Garland R. Dahlke, Sherry Hoyer, Erika L. Lundy, Lee L. Schulz, Grant A. Dewell 2016 Iowa State University

Iowa Beef Center, Dan Loy, Patrick J. Gunn, Beth E. Doran, Russ Euken, Denise L. Schwab, Chris Clark, Joe Sellers, Patrick B. Wall, Garland R. Dahlke, Sherry Hoyer, Erika L. Lundy, Lee L. Schulz, Grant A. Dewell

Animal Industry Report

The Iowa Beef Center (IBC) is Iowa State University’s extension and outreach program to cattle producers. Our center has a dedicated group of faculty and staff from the College of Agriculture and Life Sciences, the College of Veterinary Medicine, and Iowa State University Extension and Outreach. We work together to develop and deliver the latest research-based information to improve the profitability and vitality of Iowa’s beef industry.


Ring Test Evaluation Of The Detection Of Influenza A Virus In Swine Oral Fluids By Real-Time Reverse-Transcription Polymerase Chain Reaction And Virus Isolation, Christa K. Goodell, Jianqiang Zhang, Erin Strait, Karen Harmon, Devi Patnayak, Tracy Otterson, Marie Culhane, Jane Christopher-Hennings, Travis Clement, Pamela Leslie-Steen, Richard Hesse, Joe Anderson, Kevin Skarbek, Amy Vincent, Pravina Kitikoon, Sabrina Swenson, Melinda Jenkins-Moore, Jodi McGill, Rolf Rauh, William Nelson, Catherine O'Connell, Rohn Shah, Chong Wang, Rodger Main, Jeffery J. Zimmerman 2016 Iowa State University

Ring Test Evaluation Of The Detection Of Influenza A Virus In Swine Oral Fluids By Real-Time Reverse-Transcription Polymerase Chain Reaction And Virus Isolation, Christa K. Goodell, Jianqiang Zhang, Erin Strait, Karen Harmon, Devi Patnayak, Tracy Otterson, Marie Culhane, Jane Christopher-Hennings, Travis Clement, Pamela Leslie-Steen, Richard Hesse, Joe Anderson, Kevin Skarbek, Amy Vincent, Pravina Kitikoon, Sabrina Swenson, Melinda Jenkins-Moore, Jodi Mcgill, Rolf Rauh, William Nelson, Catherine O'Connell, Rohn Shah, Chong Wang, Rodger Main, Jeffery J. Zimmerman

Veterinary Diagnostic and Production Animal Medicine Publications

The probability of detecting influenza A virus (IAV) in oral fluid (OF) specimens was calculated for each of 13 assays based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) and 7 assays based on virus isolation (VI). The OF specimens were inoculated with H1N1 or H3N2 IAV and serially diluted 10-fold (10(-1) to 10(-8)). Eight participating laboratories received 180 randomized OF samples (10 replicates × 8 dilutions × 2 IAV subtypes plus 20 IAV-negative samples) and performed the rRT-PCR and VI procedure(s) of their choice. Analysis of the results with a mixed-effect logistic-regression model identified dilution and assay as variables ...


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