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Intestinal Colonization And Acute Immune Response In Commercial Turkeys Following Inoculation With Campylobacter Jejuni Constructs Encoding Antibiotic-Resistance Markers, Matthew J. Sylte, Timothy A. Johnson, Ella L. Meyer, Matt H. Inbody, Julian Trachsel, Torey Looft, Leonardo Susta, Zuowei Wu, Qijing Zhang 2019 United State Department of Agriculture

Intestinal Colonization And Acute Immune Response In Commercial Turkeys Following Inoculation With Campylobacter Jejuni Constructs Encoding Antibiotic-Resistance Markers, Matthew J. Sylte, Timothy A. Johnson, Ella L. Meyer, Matt H. Inbody, Julian Trachsel, Torey Looft, Leonardo Susta, Zuowei Wu, Qijing Zhang

Veterinary Microbiology and Preventive Medicine Publications

Consumption of contaminated poultry products is one of the main sources of human campylobacteriosis, of which Campylobacter jejuni subsp. jejuni (C. jejuni) is responsible for approximately 90% of the cases. At slaughter, the ceca of commercial chickens and turkeys are the main anatomical site where C. jejuni asymptomatically colonizes. We have previously colonized commercial turkey poults with different isolates of C. jejuni and evaluated different media to best enumerate Campylobacter from intestinal samples, but the host-response is unknown in turkeys. Enumeration of Campylobacter(colony forming units (cfu)/gram of intestinal contents) can be challenging, and can be confounded if animals ...


A Review Of Relevant Therapeutic Targets For The Management Of Congestive Heart Failure In Dogs: Present And Future Directions, Jonathan P. Mochel 2019 Iowa State University

A Review Of Relevant Therapeutic Targets For The Management Of Congestive Heart Failure In Dogs: Present And Future Directions, Jonathan P. Mochel

Jonathan Mochel

Congestive heart failure (CHF) is a major cause of morbidity and mortality with an increasing prevalence in human and canine populations. Similar to humans, overactivation of the renin-angiotensin aldosterone system is involved in the pathophysiology of CHF in dogs. Current therapeutic strategies for the management of canine CHF include the use of RAAS inhibitors, diuretics and inodilators. The present review summarizes data from our own research on the modulation of the renin-angiotensin cascade in dogs in dogs, together with new findings (including novel therapeutic targets) from the veterinary and the human literature.


Still Moving Toward Automation Of The Systematic Review Process: A Summary Of Discussions At The Third Meeting Of The International Collaboration For Automation Of Systematic Reviews (Icasr), Annette M. O'Connor, Guy Tsafnat, Stephen B. Gilbert, Kristina A. Thayer, Ian Shemilt, James Thomas, Paul Glasziou, Mary S. Wolfe 2019 Iowa State University

Still Moving Toward Automation Of The Systematic Review Process: A Summary Of Discussions At The Third Meeting Of The International Collaboration For Automation Of Systematic Reviews (Icasr), Annette M. O'Connor, Guy Tsafnat, Stephen B. Gilbert, Kristina A. Thayer, Ian Shemilt, James Thomas, Paul Glasziou, Mary S. Wolfe

Stephen B. Gilbert

The third meeting of the International Collaboration for Automation of Systematic Reviews (ICASR) was held 17–18 October 2017 in London, England. ICASR is an interdisciplinary group whose goal is to maximize the use of technology for conducting rapid, accurate, and efficient systematic reviews of scientific evidence. The group seeks to facilitate the development and widespread acceptance of automated techniques for systematic reviews. The meeting’s conclusion was that the most pressing needs at present are to develop approaches for validating currently available tools and to provide increased access to curated corpora that can be used for validation. To that ...


That Sheep May Safely Graze: Rebuilding Animal Health Care In War-Torn Afghanistan, David M. Sherman 2019 Purdue University

That Sheep May Safely Graze: Rebuilding Animal Health Care In War-Torn Afghanistan, David M. Sherman

Purdue University Press Book Previews

The very mention of Afghanistan conjures images of war, international power politics, the opium trade, and widespread corruption. Yet the untold story of Afghanistan’s seemingly endless misfortune is the disruptive impact that prolonged conflict has had on ordinary rural Afghans, their culture, and the timeless relationship they share with their land and animals. In rural Afghanistan, when animals die, livelihoods are lost, families and communities suffer, and people may perish. That Sheep May Safely Graze details a determined effort, in the midst of war, to bring essential veterinary services to an agrarian society that depends day in and day ...


Endoscopic Laser-Ablation For The Treatment Of Orthotopic And Ectopic Ureteroceles In Dogs: 13 Cases (2008-2017)., Cleo P. Rogatko, Allyson C. Berent, Larry G. Adams, Chick W. Weisse, Demetrius H. Bagley 2019 The Animal Medical Center

Endoscopic Laser-Ablation For The Treatment Of Orthotopic And Ectopic Ureteroceles In Dogs: 13 Cases (2008-2017)., Cleo P. Rogatko, Allyson C. Berent, Larry G. Adams, Chick W. Weisse, Demetrius H. Bagley

Department of Urology Faculty Papers

BACKGROUND: Ureteroceles are a rare condition in dogs in which conventional treatments can result in substantial morbidity. Cystoscopic and fluoroscopic-guided laser ablation (CLA) of ureteroceles can successfully relieve obstruction.

OBJECTIVES: To describe the technique and outcomes of attempting CLA for treatment of ureteroceles in dogs.

ANIMALS: Thirteen client-owned dogs that underwent CLA for treatment of ureteroceles.

METHODS: Retrospective multicentered study. Medical records were reviewed in all dogs that underwent CLA for ureterocele(s). A laser was used to extend the opening of the ureteral orifice (UO) unless surgical conversion was necessary. Data collected included signalment, clinicopathologic data, imaging, procedural findings ...


Pentablock Copolymer Micelle Nanoadjuvants Enhance Cytosolic Delivery Of Antigen And Improve Vaccine Efficacy While Inducing Low Inflammation, Sujata Senapati, Ross J. Darling, Darren Loh, Ian C. Schneider, Michael J. Wannemeuhler, Balaji Narasimhan, Surya K. Mallapragada 2019 Iowa State University

Pentablock Copolymer Micelle Nanoadjuvants Enhance Cytosolic Delivery Of Antigen And Improve Vaccine Efficacy While Inducing Low Inflammation, Sujata Senapati, Ross J. Darling, Darren Loh, Ian C. Schneider, Michael J. Wannemeuhler, Balaji Narasimhan, Surya K. Mallapragada

Chemistry Publications

As the focus has shifted from traditional killed or live, attenuated vaccines towards subunit vaccines, improvements in vaccine safety have been confronted with low immunogenicity of protein antigens. This issue has been addressed by synthesizing and designing a wide variety of antigen carriers and adjuvants, such as Toll-like receptor agonists (e.g., MPLA, CpG). Studies have focused on optimizing adjuvants for improved cellular trafficking, cytosolic availability, and improved antigen presentation. In this work, we describe the design of novel amphiphilic pentablock copolymer (PBC) adjuvants that exhibit high biocompatibility and reversible pH- and temperature-sensitive micelle formation. We demonstrate improved humoral immunity ...


Data Evidencing Slow Anaerobic Digestion In Emergency Treatment And Disposal Of Infectious Animal Carcasses, Jacek A. Koziel, Heekwon Ahn, Thomas D. Glanville, Timothy S. Frana, J. (Hans) van Leeuwen, Lam T. Nguyen 2019 Iowa State University

Data Evidencing Slow Anaerobic Digestion In Emergency Treatment And Disposal Of Infectious Animal Carcasses, Jacek A. Koziel, Heekwon Ahn, Thomas D. Glanville, Timothy S. Frana, J. (Hans) Van Leeuwen, Lam T. Nguyen

Agricultural and Biosystems Engineering Publications

Burial of infectious and potentially infectious livestock and poultry animals is the most common response to an emergency situation. The data set summarizes 22-week-long experiment that simulates the environment found within conventional burial trenches for emergency disposal of animal carcasses, worldwide, sometimes with a topical application of quicklime as it is required in the Republic of Korea. This data set shows the rarely presented evidence of the extremely slow decay of animal carcasses. Besides visual evidence of no visible breakdown of carcass material, i.e., carcass (or carcass quarters and coarse cuts) still resembled the initial material at the end ...


The Role Of The Gut-Brain Axis In Neurodegenerative Diseases And Relevance Of The Canine Model: A Review, Yoko Ambrosini, Dana Borcherding, Anumantha Kanthasamy, Hyun Jung Kim, Albert Jergens, Karin Allenspach, Jonathan Mochel 2019 Iowa State University

The Role Of The Gut-Brain Axis In Neurodegenerative Diseases And Relevance Of The Canine Model: A Review, Yoko Ambrosini, Dana Borcherding, Anumantha Kanthasamy, Hyun Jung Kim, Albert Jergens, Karin Allenspach, Jonathan Mochel

Biomedical Sciences Publications

Identifying appropriate animal models is critical in developing translatable in vitro and in vivo systems for therapeutic development and investigating disease pathophysiology. These animal models should have direct biological and translational relevance to the underlying disease they are supposed to mimic. Aging dogs naturally develop a cognitive decline in many aspects including learning and memory, but also exhibit human-like individual variability in the aging process. Neurodegenerative processes that can be observed in both human and canine brains include the progressive accumulation of β-amyloid (Aβ) found as diffuse plaques in the prefrontal cortex, including the gyrus proreus, the hippocampus, and in ...


Use Of Flexible Sensor To Characterize Biomechanics Of Canine Skin, Austin R. J. Downey, Jin Yan, Eric M. Zellner, Karl H. Kraus, Iris V. Rivero, Simon Laflamme 2019 University of South Carolina

Use Of Flexible Sensor To Characterize Biomechanics Of Canine Skin, Austin R. J. Downey, Jin Yan, Eric M. Zellner, Karl H. Kraus, Iris V. Rivero, Simon Laflamme

Civil, Construction and Environmental Engineering Publications

Background: Suture materials and techniques are frequently evaluated in ex vivo studies by comparing tensile strengths. However, the direct measurement techniques to obtain the tensile forces in canine skin are not available, and, therefore, the conditions suture lines undergo is unknown. A soft elastomeric capacitor is used to monitor deformation in the skin over time by sensing strain. This sensor was applied to a sample of canine skin to evaluate its capacity to sense strain in the sample while loaded in a dynamic material testing machine. The measured strain of the sensor was compared with the strain measured by the ...


Comparison Of Traditional And Alternative Curing Ingredients On Curing Reactions In A Model Meat System, Faith D. Rasmussen, Gary A. Sullivan 2019 University of Nebraska-Lincoln

Comparison Of Traditional And Alternative Curing Ingredients On Curing Reactions In A Model Meat System, Faith D. Rasmussen, Gary A. Sullivan

Nebraska Beef Cattle Reports

To meet consumer trends, alternative curing ingredients are used to replace sodium nitrite and cure accelerators. Due to the complexity of meat, it is challenging to compare traditional and alternative ingredients for curing reactions. Using a model system, sources of nitrite (traditional, sodium nitrite and alternative, cultured celery juice powder), salt, and cure accelerators (traditional, sodium erythorbate, or alternative, cherry juice powder) at ingoing sodium nitrite concentrations of 10, 50, 100, 150, or 200 ppm were evaluated for curing reactions. More complete curing reactions were indicated by a higher concentration of cured meat pigment, and lower sulfhydryl groups. Lower residual ...


Evaluation Of Lactation Demands On Nutrient Balance In Two Calving Seasons In Range Cows Grazing Sandhills Upland Range, Travis Mulliniks, Don C. Adams 2019 University of Nebraska - Lincoln

Evaluation Of Lactation Demands On Nutrient Balance In Two Calving Seasons In Range Cows Grazing Sandhills Upland Range, Travis Mulliniks, Don C. Adams

Nebraska Beef Cattle Reports

A modeling study evaluated the effects of milk production level on nutrient balance in March-and May-calving cows grazing Sandhills upland range during the breeding season. Forage quality of upland range peaks in June and steadily declines in July until November. In March-calving cows, metabolizable protein (MP) and energy were deficient by July 1 in all milking potential cows, which is exacerbated in greater milking potential cows. May-calving cows with 20 to 30 lbs of milk are predicted to enter the breeding season with a deficiency in MP and energy. In an effort to match cow type to environment in the ...


Economic Analysis Of Beef Systems, Terry J. Klopfenstein, Shebly E. Loeffelholz, Karla H. Jenkins PAS, Andrea K. Watson, Galen E. Erickson 2019 University of Nebraska - Lincoln

Economic Analysis Of Beef Systems, Terry J. Klopfenstein, Shebly E. Loeffelholz, Karla H. Jenkins Pas, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

Economic analysis of complete beef production systems is important. An analysis of 5 systems was conducted. Three of the systems were based on cows calving in March, June or August and grazing Sandhills range and corn residue. The other 2 systems were based on complete confinement feeding or confinement in the summer and corn residue grazing in the winter. The range-based systems gave similar results, however, complete confinement was not competitive. Partial confinement with stalk grazing was competitive with range-based systems. Stalk grazing is very economical and important for many cow-calf systems.


Effects Of Supplemental Soypass In Forage-Based Diets Containing Distillers Grains On Performance Of Growing Steers, Tyler J. Spore, Zachary E. Carlson, Galen E. Erickson Erickson, Terry J. Klopfenstein, Andrea K. Watson 2019 University of Nebraska - Lincoln

Effects Of Supplemental Soypass In Forage-Based Diets Containing Distillers Grains On Performance Of Growing Steers, Tyler J. Spore, Zachary E. Carlson, Galen E. Erickson Erickson, Terry J. Klopfenstein, Andrea K. Watson

Nebraska Beef Cattle Reports

SoyPass was supplemented in two grass hay diets containing 20% or 35% wet distillers grains with solubles (WDGS) to analyze the effects on growing cattle performance. The SoyPass supplement replaced 0, 30, or 60% of dietary WDGS for a total of 6 treatments with a factorial design. Substituting SoyPass into the diet did not affect average daily gain (ADG) of calves; however, calves consuming the 35% WDGS diet gained 31% more than the 20% WDGS treatment calves. Dry matter intake (DMI) and feed to gain (F:G) increased linearly in the 35% WDGS diet with the inclusion of SoyPass. In ...


Evaluation Of Corn Bran Plus Solubles On Performance And Carcass Characteristics In Finishing Diets, Shelby A. Garland, Bradley M. Boyd, F. Henry Hilscher Hilscher, Galen E. Erickson, Jim C. MacDonald, Ryan A. Mass 2019 University of Nebraska-Lincoln

Evaluation Of Corn Bran Plus Solubles On Performance And Carcass Characteristics In Finishing Diets, Shelby A. Garland, Bradley M. Boyd, F. Henry Hilscher Hilscher, Galen E. Erickson, Jim C. Macdonald, Ryan A. Mass

Nebraska Beef Cattle Reports

A finishing study was conducted to determine the effect of feeding corn bran plus solubles, a new feed resulting from a pre-fermentation fiber removal process, compared to wet distillers grains plus solubles at two levels of inclusion (20% and 40% of diet DM). Intake increased with inclusion of byproduct, and steers fed 20% wet distillers had the greatest intakes numerically. Byproduct inclusion, regardless of type, increased daily gain over the corn-based control. Feed conversions were improved with increased inclusion of both Bran + Solubles and wet distillers, and both were superior to the control. Increased inclusion of both byproducts resulted in ...


A Comparison Of Synovex One® Alone To Synovex Choice® Followed By Synovex Plus® As Implant Strategies For Finishing Heifers, Elizabeth A. Schumacher, Galen E. Erickson, D. J. Jordon Jordon, Robert J. Cooper, Tony L. Scott, Sheri Bundy, Gary Sides, Ralph M. Cleale 2019 University of Nebraska - Lincoln

A Comparison Of Synovex One® Alone To Synovex Choice® Followed By Synovex Plus® As Implant Strategies For Finishing Heifers, Elizabeth A. Schumacher, Galen E. Erickson, D. J. Jordon Jordon, Robert J. Cooper, Tony L. Scott, Sheri Bundy, Gary Sides, Ralph M. Cleale

Nebraska Beef Cattle Reports

A commercial feedlot study utilizing 1,737 crossbred heifers (initial BW 690 lb) compared the effect of two implant strategies [Synovex ONE Feedlot (day 0) or Synovex Choice (day 0) followed by Synovex Plus (day 95)] on performance and carcass characteristics. No differences were observed in carcass weight, final body weight, or gain, but heifers implanted with Synovex ONE Feedlot had slightly greater feed conversion and greater intake than heifers implanted using Synovex Choice/Synovex Plus. Heifers implanted with Synovex Choice/Synovex Plus had lower marbling score and yield grade, higher dressing percentage, and greater loin muscle area compared to ...


Impact Of Shade In Beef Feedyards On Performance, Body Temperature, And Heat Stress Measures, Brett A. Melton, Bradley M. Boyd, Casey Macken Macken, Andrea K. Watson, James C. MacDonald, Galen E. Erickson 2019 University of Nebraska-Lincoln

Impact Of Shade In Beef Feedyards On Performance, Body Temperature, And Heat Stress Measures, Brett A. Melton, Bradley M. Boyd, Casey Macken Macken, Andrea K. Watson, James C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A study using crossbred steers was conducted at a commercial feedyard to determine the effects of pen shades on cattle performance, body temperature, and cattle activity. Two heat events (Event 1 and Event 2) and one cool event were defined for the feeding period. No significant differences were observed for average daily gain, dry matter intake, feed to gain, or carcass characteristics at the end of the trial. During Event 1, cattle in pens with shade had lower panting scores than cattle in open pens. During Event 2, cattle in shade pens had greater dry matter intake, lower panting scores ...


Effects Of Maternal Late Gestation Nutrition On May-Born Steer Progeny, Alicia C. Lansford, Jacki A. Musgrave, T. L. Meyer Meyer, Rick N. Funston 2019 University of Nebraska–Lincoln

Effects Of Maternal Late Gestation Nutrition On May-Born Steer Progeny, Alicia C. Lansford, Jacki A. Musgrave, T. L. Meyer Meyer, Rick N. Funston

Nebraska Beef Cattle Reports

May-calving dams were assigned to graze either sub-irrigated meadow or upland range with or without supplementation (1 lb/d 33% CP). Treatment began at approximately 160 d of gestation. Supplementation continued for 85 ± 2 d, while grazing system treatment continued for 116 ± 2 d. Steer progeny were backgrounded after weaning and then assigned to either a short or long yearling feedlot system. Dam supplementation tended to decrease marbling score within both feedlot systems. Short yearling steers had an increased percentage of carcasses grade USDA low Choice or greater if their dams grazed meadow in late gestation. Long yearling steers had ...


Pooled Analysis Of Individually Fed Finishing Trials, Brett A. Melton, James C. MacDonald, Andrea K. Watson Watson, Galen E. Erickson 2019 University of Nebraska - Lincoln

Pooled Analysis Of Individually Fed Finishing Trials, Brett A. Melton, James C. Macdonald, Andrea K. Watson Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

A pooled analysis of 21 finishing trials (2002–2016; 1530 animals) with cattle individually fed in Calan gate barns was conducted. Mixed model regression analysis following random coefficient methodology was used to evaluate relationships between performance variables and carcass characteristics. Gain had a greater effect on efficiency (R2 = 0.72) compared to intake (R2 = 0.02). The relationship between gain and efficiency was cubic, while intake had a quadratic relationship. The cubic response of gain relative to efficiency was continually increasing with relatively slight curves in the line heavily influenced by points that lay on the ends of ...


Three Year Summary: Comparison Of Diets Collected From Esophageally Fistulated Cows To Forage Quality Estimated From Fecal Analysis, Bethany M. Johnston, Jay D. Jenkins, Jacki A. Musgrave Musgrave, J. Travis Mulliniks, Mitchell B. Stephenson, James C. MacDonald, L. Aaron Stalker 2019 University of Nebraska-Lincoln

Three Year Summary: Comparison Of Diets Collected From Esophageally Fistulated Cows To Forage Quality Estimated From Fecal Analysis, Bethany M. Johnston, Jay D. Jenkins, Jacki A. Musgrave Musgrave, J. Travis Mulliniks, Mitchell B. Stephenson, James C. Macdonald, L. Aaron Stalker

Nebraska Beef Cattle Reports

Inconsistency was found in forage quality (crude protein and energy) when esophageally fistulated diets were compared to Nutrition Balance Analyzer (NUTBAL) analysis of fecal samples. On upland range sites, hand-clipping of samples (not a recommended practice to measure forage quality), was closer to fistulated diets than NUTBAL analysis. If cattle managers are solely utilizing NUTBAL for estimates of forage value, incorrect supplemental energy and protein decisions will likely be made resulting in the purchase of unnecessary supplements, thereby reducing the profitability of the operation.


Nitrate Concentrations Of Annual Forages Grown For Grazing In Nebraska, Mary E. Lenz, Rebecca J. Kern, Carrie E. Orvis Orvis, Mary E. Drewnoski 2019 University of Nebraska, Lincoln

Nitrate Concentrations Of Annual Forages Grown For Grazing In Nebraska, Mary E. Lenz, Rebecca J. Kern, Carrie E. Orvis Orvis, Mary E. Drewnoski

Nebraska Beef Cattle Reports

Annual forage samples sent by producers to Ward Laboratories Inc. for nitrate analysis were evaluated to determine which cover crop species in Nebraska are most likely to accumulate nitrates, and how often the accumulated nitrates are considered toxic by traditional recommendations. Additionally, nitrate levels of cover crop mixes grown in research trials were analyzed to ensure species differences were repeated when grown together in the same fields. Brassicas accumulate more nitrate than small grains, millet, sorghum/sudan grasses, or cover crop mixes. Brassicas accumulated levels of nitrate considered moderately to highly toxic in 48% of the samples. The other cover ...


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