Risk Assessment For Food Safety: Application And Evaluation Of Haccp In Hog Slaughter And Processing, 2015 Iowa State University
Risk Assessment For Food Safety: Application And Evaluation Of Haccp In Hog Slaughter And Processing, Helen H. Jensen, George W. Beran, Terry Proescholdt, Laurian Unnevehr, James S. Dickson, Patricia Batres-Marquez
Iowa Ag Review
New federal regulations focus control at the processing plant level. The project described in this article is designed to evaluate the microbial reductions and costs associated with the use of a Hazard Analysis and Critical Control Point (HACCP) system in large pork slaughter and processing plants. The objectives are to measure the efficiency of HACCP systems in achieving lower microbial counts in pork processing, to measure the marginal costs associated with different levels of pathogen reduction in pork processing and to determine implications for mandated HACCP adoption on industry costs. The study considers specific control points or technologies that are ...
Beef Cattle Management Systems For Estrus Synchronization And Heifer Development, 2015 University of Nebraska-Lincoln
Beef Cattle Management Systems For Estrus Synchronization And Heifer Development, Hazy Rae Nielson
Theses and Dissertations in Animal Science
Four experiments were conducted to evaluate estrus synchronization and heifer development systems. In the first experiment delaying AI 19 h following GnRH administration in a hybrid estrus detection and time AI protocol was evaluated. Final pregnancy rate was not different among heifers detected in estrus, AI at the time GnRH administration, or delayed AI. The second study compared the effect of melengestrol acetate (MGA) -PG and 14-d controlled internal drug release (CIDR) -PG estrus synchronization protocols on estrus response and pregnancy rates of 311 d old heifers. Final pregnancy rate was similar between CIDR and MGA treatment groups. The objective ...
Evaluation Of An Interactive Workshop Designed To Teach Practical Welfare Techniques To Beef Cattle Caretakers And Decision Makers, Reneé Dewell, Christy J. Hanthorn, Jared A. Danielson, Rebecca G. Burzette, Johann F. Coetzee, D. Dee Griffin, Alejandro Ramirez, Grant A. Dewell
Veterinary Diagnostic and Production Animal Medicine Publications
The purpose of the project was to evaluate the use of an interactive workshop designed to teach novel practical welfare techniques to beef cattle caretakers and decision makers. Following training, respondents reported being more likely to use or recommend use of local anesthesia for dehorning and castration and were more inclined to use meloxicam for pain management. Respondents also reported improvement in performing all skills taught in the workshop. These results suggest instructional techniques used to teach practical concepts/techniques relevant to beef cattle welfare led to improvements in both perceived competency and estimated likelihood that the material will be ...
Research Data Practices In Veterinary Medicine: A Case Study, 2015 University of Illinois at Urbana-Champaign
Research Data Practices In Veterinary Medicine: A Case Study, Erin E. Kerby
Journal of eScience Librarianship
Objective: To determine trends in research data output, reuse, and sharing of the college of veterinary medicine faculty members at a large academic research institution.
Methods: This bibliographic study was conducted by examining original research articles for indication of the types of data produced, as well as evidence that the authors reused data or made provision for sharing their own data. Findings were recorded in the categories of research type, data type, data reuse, data sharing, author collaboration, and grants/funding and were analyzed to determine trends.
Results: A variety of different data types were encountered in this study, even ...
Repurposing Celecoxib As A Topical Antimicrobial Agent, 2015 Purdue University
Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem
Department of Comparative Pathobiology Faculty Publications
There is an urgent need for new antibiotics and alternative strategies to combat multidrug-resistant bacterial pathogens, which are a growing clinical issue. Repurposing existing approved drugs with known pharmacology and toxicology is an alternative strategy to accelerate antimicrobial research and development. In this study, we show that celecoxib, a marketed inhibitor of cyclooxygenase-2, exhibits broad-spectrum antimicrobial activity against Gram-positive pathogens from a variety of genera, including Staphylococcus, Streptococcus, Listeria, Bacillus, and Mycobacterium, but not against Gram-negative pathogens. However, celecoxib is active against all of the Gram-negative bacteria tested, including strains of, Acinetobacter, and Pseudomonas, when their intrinsic resistance is artificially ...
In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, 2015 Southern Illinois University Edwardsville
In Vitro And In Vivo Evaluation Of Infestation Deterrents Against Lice, Kyong-Sup Yoon, Jennifer K. Ketzis, Samuel W. Andrewes, Christopher S. Wu, Kris Honraet, Dorien Staljanssens, Bart Rossel, J Marshall Clark
SIUE Faculty Research, Scholarship, and Creative Activity
The human head louse is a cosmopolitan ectoparasite and frequently infests many people, particularly school-age children. Due to widespread pyrethroid resistance and the lack of efficient resistance management, there has been a considerable interest in the protection of uninfested people and prevention of reinfestation by disrupting lice transfer. In this study, two nonclinical model systems (in vitro and in vivo) were used to determine the efficacy of the infestation deterrents, Elimax lotion and Elimax shampoo, against human head lice or poultry chewing lice, respectively. With in vitro assessments, female head lice exhibited significantly higher avoidance responses to hair tufts treated ...
Animal Health Matters, 2015 South Dakota State University
Animal Health Matters, Russ Daly, Jane Hennings
Animal Health MATTERS Newsletter
[Page] 1- ADRDL Steps Up in Light of Highly Pathogenic H5N2 Avian Influenza Outbreaks [Page] 2- Director's Message: The SD Animal Disease Research and Diagnostic Laboratory and Emergency Testing for Highly Pathogenic Avian Influenza [Page] 3- Pre-Veterinary Students Honored by SDVMA and SDSU Through Stethoscope Ceremony [Page] 4- Clerical Staff Makes Strides in Efficiency and Service [Page] 5- ADRDL Pathologists Team with Veterinarian to Address Cattle Lameness for Livestock Group [Page] 5- Canine Influenza Testing—SDSU ADRDL [Page] 6- ADRDL Hosts Tours for Legislators and State Agency Staff [Page] 6- ADRDL Offers Next Generation Sequencing [Page] 6- ADRDL at ...
Fecal Volatile Organic Ccompound Profiles From White-Tailed Deer (Odocoileus Virginianus) As Indicators Of Mycobacterium Bovis Exposure Or Mycobacterium Bovis Bacille Calmette-Guerin (Bcg) Vaccination, 2015 U.S. Department of Agriculture
Fecal Volatile Organic Ccompound Profiles From White-Tailed Deer (Odocoileus Virginianus) As Indicators Of Mycobacterium Bovis Exposure Or Mycobacterium Bovis Bacille Calmette-Guerin (Bcg) Vaccination, Randal S. Stahl, Christine K. Ellis, Pauline Nol, W. Ray Waters, Kurt C. Vercauteren
USDA National Wildlife Research Center - Staff Publications
White-tailed deer (Odocoileus virginianus) serve as a reservoir for bovine tuberculosis, caused by Mycobacterium bovis, and can be a source of infection in cattle. Vaccination with M. bovis Bacille Calmette Guerin (BCG) is being considered for management of bovine tuberculosis in deer. Presently, no method exists to non-invasively monitor the presence of bovine tuberculosis in deer. In this study, volatile organic compound profiles of BCG-vaccinated and non-vaccinated deer, before and after experimental challenge with M. bovis strain 95–1315, were generated using solid phase microextraction fiber head-space sampling over suspended fecal pellets with analysis by gas chromatography/mass spectrometry. Chromatograms ...
Development Of An In Vivo Exsheathment Assay Of Infective L3 Haemonchus Contortus Larvae In Fistualated Sheep, 2015 University of Rhode Island
Development Of An In Vivo Exsheathment Assay Of Infective L3 Haemonchus Contortus Larvae In Fistualated Sheep, Holly N. Williams, Katherine Petersson, Carly Barone
Senior Honors Projects
Purpose: Gastrointestinal nematodes (GIN) hinder the sustainable production of small ruminants on pasture and parasite resistance to chemical dewormers is becoming a growing concern. Condensed tannin containing legume forages are being tested to evaluate their anti-parasitic properties and potential contribution to an overall parasite control program for small ruminants such as sheep and goats. One of the most pathogenic GIN of small ruminants is Haemonchus contortus. The final step to full infectivity of H. contortus third stage larvae (L3) is exsheathment in the rumen. The objective of this study was to establish an in-vivo exsheathment assay in fistulated sheep as ...
Novel Clostridium Species, From The Swine Intestinal Tract, 2015 Iowa State University
Novel Clostridium Species, From The Swine Intestinal Tract, Sarah Azam
Symposium on Undergraduate Research and Creative Expression
The swine intestinal tract is one of the most densely populated microbial ecosystems identified. The microbiota greatly impact host physiology, metabolism, and immunity. However a large portion of intestinal microorganisms remain unidentified. Characterization of unclassified members fills an important gap in understanding the microbiota composition and function. To address this need, we isolated and characterized a novel bacterium (strain 68-1-5) from the swine intestinal tract on mucusbased media. This gram-positive bacterium belongs to the genus Clostridium, which includes 150 characterized species. In the intestinal compartment, these organisms promote intestinal health by stimulating the immune system and producing compounds beneficial to ...
Identification Of Mycoplasma Spp. Using Maldi-Tof Mass Spectrometry, 2015 Iowa State University
Identification Of Mycoplasma Spp. Using Maldi-Tof Mass Spectrometry, Aric Mcdaniel
Symposium on Undergraduate Research and Creative Expression
Mycoplasma spp. cause a variety of diseases in cattle including pneumonia, arthritis, and mastitis. Mycoplasma bovis, the most pathogenic species in cattle, has been shown to be accurately identified using Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. After confirming the identity of 13 Mycoplasma standard organisms, 35 Mycoplasma case isolates were run through MALDI-TOF. Of the 35 isolates, 13 had no reliable identification (NRID), 12 identified as M. bovis, and 9 identified as a non-M. bovis species. All 12 isolates identified as M. bovis were confirmed by an M. bovis-specific PCR, and 7 of the non-M. bovis isolates were ...
A Behavioral Analysis Of The Pre-Release Tasmanian Devils (Sarcophilus Harrisii) Currently At Devils@Cradle: An Assessment Of The Impacts Of Captivity On The Wild Behaviors Of Tasmanian Devils That Are Important For Their Reintroduction, Survival, And Inclusion In Wild Populations, 2015 SIT Study Abroad
A Behavioral Analysis Of The Pre-Release Tasmanian Devils (Sarcophilus Harrisii) Currently At Devils@Cradle: An Assessment Of The Impacts Of Captivity On The Wild Behaviors Of Tasmanian Devils That Are Important For Their Reintroduction, Survival, And Inclusion In Wild Populations, Anna Staudenmaier
Independent Study Project (ISP) Collection
The Tasmanian devil, Sarcophilus harrisi, is in danger of extinction in the wild due to the emergence of Devil Facial Tumor Disease (DFTD). In an attempt to save the species the Save the Tasmanian Devil Program (STTDP) has initiated the creation of an ‘Insurance Population’. These insurance animals are a part of the captive breeding population (CBP) designed to conserve the genetic diversity of the species to preserve their ecological function for their future reintroduction into the wild. CBPs are located at various bio-secure zoos, wildlife parks, free-range enclosures (FREs), Tasmanian islands and peninsulas and sanctuaries like Devils@Cradle - Tasmanian ...
Detection Of Streptococus Equi From Environmental Samples, 2015 University of Maine - Main
Detection Of Streptococus Equi From Environmental Samples, Taryn Haller
Equine Strangles, an upper respiratory disease caused by Streptococcus equi subspecies equi (S. equi) causes significant losses in the horse industry. Surveillance for S. equi could be facilitated by quantifying S. equi in environmental samples. The objective of this project was to evaluate ELISA in quantifying S. equi using two monoclonal antibodies (MAB’s) to the SeM protein (MAB-212 for capture, and biotinylated MAB-211 for detection), with Streptococcal phage lysin releasing SeM from the cell surface. Initial results confirmed a fresh culture of S. equi and two S. equi lysates stored at -20 C for 2 years as positive, while ...
Animal Health Matters, 2015 South Dakota State University
Animal Health Matters, Russ Daly, Jane Hennings
Animal Health MATTERS Newsletter
[Page] 1- Latest Version of South Dakota “One Health” Meetings Explores Tuberculosis in Humans and Cattle [Page] 2- Director's Message [Page] 2- Bailey Herd Health Conference Covers Connection Between Facilities and Animal Health [Page] 3- Essential Behind the Scenes Work: Accounting and Human Resources Staff at the VBS and ADRDL [Page] 3- New Hires at South Dakota ADRDL [Page] 4- Dairy Animal and Product Testing Services at the ADRDL [Page] 6- Video Production Highlights ADRDL Value to Animals and Citizens [Page] 6- Bovine Respiratory Coronavirus Testing [Page] 6- Dr. Hennings Named to AAVLD Executive Board [Page] 6- Equine Well-Being ...
Relationship Between Heat-Labile Enterotoxin Secretion Capacity And Virulence In Wild Type Porcine-Origin Enterotoxigenic Escherichia Coli Strains, 2015 University of Nebraska-Lincoln
Relationship Between Heat-Labile Enterotoxin Secretion Capacity And Virulence In Wild Type Porcine-Origin Enterotoxigenic Escherichia Coli Strains, Prageeth Wijemanne, Jun Xing, Emil M. Berberov, David B. Marx, David H. Francis, Rodney A. Moxley
Papers in Veterinary and Biomedical Science
Heat-labile enterotoxin (LT) is an important virulence factor secreted by some strains of enterotoxigenic Escherichia coli (ETEC). The prototypic human-origin strain H10407 secretes LT via a type II secretion system(T2SS). We sought to determine the relationship between the capacity to secrete LT and virulence in porcine-origin wild type (WT) ETEC strains. Sixteen WT ETEC strains isolated from cases of severe diarrheal disease were analyzed by GM1ganglioside enzyme- linked immunosorbent assay tomeasure LT concentrations in culture supernatants. All strains had detectable LT in supernatants by 2 h of culture and 1 strain, which was particularly virulent in gnotobiotic piglets (3030-2 ...
Species-Specific And Pathotype-Specific Binding Of Bacteria To Zymogen Granule Membrane Glycoprotein 2 (Gp2), 2015 Brandenburgische Technische Universität Cottbus-Senftenberg
Species-Specific And Pathotype-Specific Binding Of Bacteria To Zymogen Granule Membrane Glycoprotein 2 (Gp2), Peter Schierack, Stefan Rödiger, Rafal Kolenda, Rico Hiemann, Enrico Berger, Krzysztof Grzymajło, Alexander Swidsinski, Thomas Juretzek, Dirk Meissner, Karsten Mydlak, Dirk Reinhold, Lisa K. Nolan, Dirk Roggenbuck
Veterinary Microbiology and Preventive Medicine Publications and Papers
With interest we read the paper by Juste et al 1 proposing the amount of zymogen-granule membrane glycoprotein 2 (GP2) on the surface of intestinal bacteria as a Crohn's disease (CD) marker. Indeed, a decreased GP2 level was found on microbes in patients with CD as compared to those of healthy controls. GP2 is a homologue to the urinary Tamm–Horsefall protein demonstrating an antimicrobial function by binding type 1-fimbriated uropathogenic Escherichia coli (UPEC). Likewise, GP2 seems to interact with intestinal bacteria as a specific receptor of bacterial type-1 fimbriae (FimH) on intestinal microfold cells that are partaking in ...
Exclusion Of The Unfolded Protein Response In Light-Induced Retinal Degeneration In The Canine T4r Rho Model Of Autosomal Dominant Retinitis Pigmentosa, Stefania Marsilli, Sem Genini, Raghavi Sudharsan, Jeremy Gingrich, Gustavo D. Aguirre, William Beltran
Departmental Papers (Vet)
To examine the occurrence of endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) following acute light damage in the naturally-occurring canine model of RHO-adRP (T4R RHOdog).
The left eyes of T4R RHOdogs were briefly light-exposed and retinas collected 3, 6 and 24 hours later. The contra-lateral eyes were shielded and used as controls. To evaluate the time course of cell death, histology and TUNEL assays were performed. Electron microscopy was used to examine ultrastructural alterations in photoreceptors at 15 min, 1 hour, and 6 hours after light exposure. Gene expression of markers of ...
Plasma Concentrations Of Fenbendazole (Fbz) And Oxfendazole In Alpacas (Lama Pacos) After Single Intravenous And Oral Dosing Of Fbz, 2015 University of Tennessee - Knoxville
Plasma Concentrations Of Fenbendazole (Fbz) And Oxfendazole In Alpacas (Lama Pacos) After Single Intravenous And Oral Dosing Of Fbz, Jeffrey Lakritz, Daniel Linden, David E. Anderson, Terri A. Specht
David Edgar Anderson, DVM, MS, DACVS
The objective of this study was to determine plasma pharmacokinetics and bioavailability of fenbendazole (FBZ) and oxfendazole (OFZ) after intravenous (iv) and oral administrations of FBZ (5 mg/kg) to alpacas. Plasma concentrations of FBZ and OFZ after administration of FBZ iv and orally (5 mg/kg) were determined by high-performance liquid chromatography with ultraviolet detection. Total clearance (CL) of FBZ was 16.5±4 mL/kg/min (range: 4–31 mL/kg/min), and steady-state volume of distribution (Vdss) was 3.3±1 L/kg (range: 1.7–7.4 L/kg). The terminal phase half-life of FBZ after ...
Systems-Based Analysis Of The Sarcocystis Neurona Genome Identifies Pathways That Contribute To A Heteroxenous Life Cycle, 2015 Hospital for Sick Children, Canada
Systems-Based Analysis Of The Sarcocystis Neurona Genome Identifies Pathways That Contribute To A Heteroxenous Life Cycle, Tomasz Blazejewski, Nirvana Nursimulu, Viviana Pszenny, Sriveny Dangoudoubiyam, Sivaranjani Namasivayam, Melissa A. Chiasson, Kyle Chessman, Michelle Tonkin, Lakshmipuram S. Swapna, Stacy S. Hung, Joshua Bridgers, Stacy M. Ricklefs, Martin J. Boulanger, Jitender P. Dubey, Stephen F. Porcella, Jessica C. Kissinger, Daniel K. Howe, Michael E. Grigg, John Parkinson
Veterinary Science Faculty Publications
Sarcocystis neurona is a member of the coccidia, a clade of single-celled parasites of medical and veterinary importance including Eimeria, Sarcocystis, Neospora, and Toxoplasma. Unlike Eimeria, a single-host enteric pathogen, Sarcocystis, Neospora, and Toxoplasma are two-host parasites that infect and produce infectious tissue cysts in a wide range of intermediate hosts. As a genus, Sarcocystis is one of the most successful protozoan parasites; all vertebrates, including birds, reptiles, fish, and mammals are hosts to at least one Sarcocystis species. Here we sequenced Sarcocystis neurona, the causal agent of fatal equine protozoal myeloencephalitis. The S. neurona genome is 127 Mbp, more ...
Ectoparasitism Shortens The Breeding Season In A Colonial Bird, 2015 Department of Biological Sciences, University of Tulsa
Ectoparasitism Shortens The Breeding Season In A Colonial Bird, Charles R. Brown, Mary Bomberger Brown
Papers in Natural Resources
When blood-feeding parasites increase seasonally, their deleterious effects may prevent some host species, especially those living in large groups where parasites are numerous, from reproducing later in the summer. Yet the role of parasites in regulating the length of a host’s breeding season—and thus the host’s opportunity for multiple brooding—has not been systematically investigated. The highly colonial cliff swallow (Petrochelidon pyrrhonota), a temperate-latitude migratory songbird in the western Great Plains, USA, typically has a relatively short (eight to nine week) breeding season, with birds rarely nesting late in the summer. Colonies at which ectoparasitic swallow bugs ...