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Taylor, Charles Forman, Jr., 1862-1946 (Sc 2975), Manuscripts & Folklife Archives 2016 Western Kentucky University

Taylor, Charles Forman, Jr., 1862-1946 (Sc 2975), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 2975. Letter written by Charles Forman Taylor Jr., Bowling Green, Kentucky to Joe Werthan, Nashville, Tennessee, describing a cough syrup to be used on Mr. Werthan’s horses. The letter includes a list of Taylor’s other equine medications with prices.


The ‘Prexcel-Q Method’ For Qpcr, Jack Gallup, Mark Ackermann 2016 Iowa State University

The ‘Prexcel-Q Method’ For Qpcr, Jack Gallup, Mark Ackermann

Jack M Gallup

The purpose of this manuscript is to describe a reliable approach to quantitative real-time polymerase chain reaction (qPCR) assay development and project management, which is currently embodied in the Excel 2003-based software program named “PREXCEL-Q” (P-Q) (formerly known as “FocusField2-6Gallup-qPCRSet-upTool-001,” “FF2-6-001 qPCR set-up tool” or “Iowa State University Research Foundation [ISURF] project #03407”). Since its inception from 1997-2007, the program has been well-received and requested around the world and was recently unveiled by its inventor at the 2008 Cambridge Healthtech Institute’s Fourth Annual qPCR Conference in San Diego, CA. P-Q was subsequently mentioned in a review article by Stephen ...


Increased Concentration Of Iodide In Airway Secretions Is Associated With Reduced Rsv Disease Severity, Rachel Derscheid, Albert van Geelen, Abigail Berkebile, Jack Gallup, Shannon Hostetter, Botond Banfi, Paul McCray, Mark Ackermann 2016 Iowa State University

Increased Concentration Of Iodide In Airway Secretions Is Associated With Reduced Rsv Disease Severity, Rachel Derscheid, Albert Van Geelen, Abigail Berkebile, Jack Gallup, Shannon Hostetter, Botond Banfi, Paul Mccray, Mark Ackermann

Jack M Gallup

Recent studies have revealed that the human and non-rodent mammalian airway mucosa contains an oxidative host defense system. This three-component system consists of hydrogen peroxide (H2O2)-producing enzymes dual oxidase (Duox) 1 and 2, thiocyanate (SCN-), and secreted lactoperoxidase (LPO). The LPO-catalyzed reaction between H2O2 and SCN- yields the bactericidal hypothiocyanite (OSCN-) in airway surface liquid (ASL). Although SCN- is the physiological substrate of LPO, the Duox/LPO/halide system can also generate hypoiodous acid (HOI) when the iodide (I-) concentration is elevated in ASL. Since HOI, but not OSCN-, inactivates respiratory syncytial virus (RSV) in cell culture, we used ...


Differential Expression Of Sheep Beta-Defensin-1 And -2 And Interleukin 8 During Acute Mannheimia Haemolytica Pneumonia, Mark Ackermann, Jack Gallup, Joseph Zabner, Richard Evans, Charles Brockus, David Meyerholz, Branka Grubor, Kim Brogden 2016 Iowa State University

Differential Expression Of Sheep Beta-Defensin-1 And -2 And Interleukin 8 During Acute Mannheimia Haemolytica Pneumonia, Mark Ackermann, Jack Gallup, Joseph Zabner, Richard Evans, Charles Brockus, David Meyerholz, Branka Grubor, Kim Brogden

Jack M Gallup

Beta-defensins are antimicrobial peptides produced by several cell types, including respiratory epithelia and leukocytes. Expression of some beta-defensins is increased by bacterial-induced inflammatory responses whereas expression of other beta-defensins is constitutive. Two beta-defensins are expressed in lungs of sheep (sheep beta-defensin-1 and -2; SBD-1/-2) and expression of SBD-1 is increased during parainfluenza virus type 3 (PI-3) infection. The effect of Mannheimia haemolytica, a Gram-negative bacteria known to induce expression of bovine beta-defensins and NF-kappa B in lung, has not been determined for SBD-1/-2. In this study, different concentrations of M. haemolytica were inoculated into pulmonary bronchi of lambs ...


Exogenous Administration Of Vascular Endothelial Growth Factor Prior To Human Respiratory Syncytial Virus A2 Infection Reduces Pulmonary Pathology In Neonatal Lambs And Alters Epithelial Innate Immune Responses, Alicia Olivier, Jack Gallup, Albert Van Geelen, Mark Ackermann 2016 Iowa State University

Exogenous Administration Of Vascular Endothelial Growth Factor Prior To Human Respiratory Syncytial Virus A2 Infection Reduces Pulmonary Pathology In Neonatal Lambs And Alters Epithelial Innate Immune Responses, Alicia Olivier, Jack Gallup, Albert Van Geelen, Mark Ackermann

Jack M Gallup

Human respiratory syncytial virus (RSV) affects thousands of children every year. Vascular endothelial growth factor (VEGF) is a regulator of vasculogenesis, pulmonary maturation, and immunity. In order to test the extent to which VEGF may alter RSV infection, 4 groups of lambs received either human recombinant VEGF (rhVEGF) or phosphate-buffered saline (PBS) pretreatment followed by inoculation with human RSV strain A2 or sterile medium. Lambs in each group were sacrificed at 2, 4, and 6 days post infection. Expression of surfactant protein-A (SP-A), surfactant protein-D (SP-D), sheep β-defensin-1 (SBD-1), tumor necrosis factor α (TNFα), interleukin (IL)-6, IL-8, interferon β ...


Repeated Intravenous Doses Of All-Trans-Retinoyl Β-D-Glucuronide Is Not Effective In The Treatment Of Bacterial Bronchopneumonia In Lambs But Is Devoid Of Gross And Acute Toxicity, Jack Gallup, Branka Grubor, Arun Barua, Gholamreza Mohammadi, Kim Brogden, James Olson, Mark Ackermann 2016 Iowa State University

Repeated Intravenous Doses Of All-Trans-Retinoyl Β-D-Glucuronide Is Not Effective In The Treatment Of Bacterial Bronchopneumonia In Lambs But Is Devoid Of Gross And Acute Toxicity, Jack Gallup, Branka Grubor, Arun Barua, Gholamreza Mohammadi, Kim Brogden, James Olson, Mark Ackermann

Jack M Gallup

Background
All-trans-retinoyl β-D-glucuronide, a water-soluble glucuronic acid conjugate of all-transretinoic acid, has demonstrated high biological activity and low toxicity in most in vitro and in vivo models. Since the reparative effects of retinoids on epithelium are well-known, our aim was to study the effect(s) of intravenously-administered all-trans-retinoyl β-D-glucuronide in lambs with chronic bacterial bronchopneumonia.

Material/Methods
Two groups of lambs were inoculated intrabronchially with either pyrogen-free saline or Mannheimia haemolytica. Thirty-three days later, lambs were injected four times at five-day intervals with 2 mL of 116 mM all-trans-retinoyl β-D-glucuronide (6.0–9.3 µmol/kg or 2.86–4 ...


Reduction Of Pulmonary Mast Cells In Areas Of Acute Inflammation In Calves With Mannheimia (Pasteurella) Haemolytica Pneumonia, Rafael Ramírez-Romero, Kim Brogden, Jack Gallup, Richard Dixon, Mark Ackermann 2016 Iowa State University

Reduction Of Pulmonary Mast Cells In Areas Of Acute Inflammation In Calves With Mannheimia (Pasteurella) Haemolytica Pneumonia, Rafael Ramírez-Romero, Kim Brogden, Jack Gallup, Richard Dixon, Mark Ackermann

Jack M Gallup

Mast cells in the left cranial pulmonary lobe of colostrum-deprived neonatal calves were quantified 2 and 6 h after intrabronchial inoculation with Mannheimia (Pasteurella) haemolytica A1. The mast cells were detected (1) immunohistochemically with a mouse anti-human mast cell tryptase monoclonal antibody, and (2) by metachromatic staining with low pH toluidine blue. A greater number of mast cells was demonstrated by the second method than by the first. At 6 h after inoculation, but not at 2 h, the number of mast cells was significantly reduced at the site of the main lesions. Treatment of calves with a sialyl Lewis ...


Influence Of Β2-Integrin Adhesion Molecule Expression And Pulmonary Infection With Pasteurella Haemolytica On Cytokine Gene Expression In Cattle, Haa-Yung Lee, Marcus Kehrli, Kim Brogden, Jack Gallup, Mark Ackermann 2016 United States Department of Agriculture

Influence Of Β2-Integrin Adhesion Molecule Expression And Pulmonary Infection With Pasteurella Haemolytica On Cytokine Gene Expression In Cattle, Haa-Yung Lee, Marcus Kehrli, Kim Brogden, Jack Gallup, Mark Ackermann

Jack M Gallup

β2-Integrins are leukocyte adhesion molecules composed of alpha (CD11a, -b, -c, or -d) and beta (CD18) subunit heterodimers. Genetic CD18 deficiency results in impaired neutrophil egress into tissues that varies between conducting airways and alveoli of the lung. In this study, we investigated whether CD18 deficiency in cattle affects proinflammatory cytokine (PIC) expression in pulmonary tissue after respiratory infection with Pasteurella haemolytica. Cattle were infected with P. haemolyticavia fiberoptic deposition of organisms into the posterior part of the right cranial lung lobe. Animals were euthanized at 2 or 4 h postinoculation (p.i.), and tissues were collected ...


Exposure To Ethanol During The Last Trimester Of Pregnancy Alters The Maturation And Immunity Of The Fetal Lung, Tatjana Lazic, Fatoumata Sow, Albert Van Geelen, David Meyerholz, Jack Gallup, Mark Ackermann 2016 Iowa State University

Exposure To Ethanol During The Last Trimester Of Pregnancy Alters The Maturation And Immunity Of The Fetal Lung, Tatjana Lazic, Fatoumata Sow, Albert Van Geelen, David Meyerholz, Jack Gallup, Mark Ackermann

Jack M Gallup

The effects of ethanol exposure on fetal lungs remain under investigation. Previously, we demonstrated that lambs exposed to ethanol during gestation had impaired expression of pulmonary surfactant protein A, a crucial component of lung immunity. In this study, we investigated the effects of in utero exposure to ethanol on maturation and immunity of the fetal lung. Pregnant ewes were surgically implanted with an abomasal cannula and administered 1 g ethanol/kg (n = 8) or water (n = 8) during the last trimester of pregnancy. Lambs were delivered prematurely or naturally. Neonatal lungs were assessed for maturation markers (hypoxia-inducible factor-1α [HIF-1α], HIF-2α ...


Cellular Distribution Of Anionic Antimicrobial Peptide In Normal Lung And During Acute Pulmonary Inflammation, Amanda Fales-Williams, Kim Brogden, E. Huffman, Jack Gallup, Mark Ackermann 2016 Iowa State University

Cellular Distribution Of Anionic Antimicrobial Peptide In Normal Lung And During Acute Pulmonary Inflammation, Amanda Fales-Williams, Kim Brogden, E. Huffman, Jack Gallup, Mark Ackermann

Jack M Gallup

Anionic peptides (APs) are small antimicrobial peptides present in human and ovine lung. In this study APs were also detected in bovine lung, and production of APs in lungs with acute inflammation induced by various stimuli was determined. The distribution and intensity of APs were determined by immunohistochemistry in lungs of 1) neonatal calves (1-3 days of age) inoculated with Mannheimia (Pasteurella) haemolytica, a known inducer of the bovine β-defensin lingual antimicrobial peptide (LAP) or pyrogen-free saline (PFS), and 2) growing calves (3 months of age) similarly inoculated with M. haemolytica, a lipopolysaccharide (LPS) from M. haemolytica, an LPS-associated protein ...


Neonatal Ovine Pulmonary Dendritic Cells Support Bovine Respiratory Syncytial Virus Replication With Enhanced Interleukin (Il)-4 And Il-10 Gene Transcripts, Sasha Fach, David Meyerholz, Jack Gallup, Mark Ackermann, Howard Lehmkuhl, Randy Sacco 2016 Iowa State University

Neonatal Ovine Pulmonary Dendritic Cells Support Bovine Respiratory Syncytial Virus Replication With Enhanced Interleukin (Il)-4 And Il-10 Gene Transcripts, Sasha Fach, David Meyerholz, Jack Gallup, Mark Ackermann, Howard Lehmkuhl, Randy Sacco

Jack M Gallup

The lung microenvironment is constantly exposed to microorganisms and particulate matter. Lung dendritic cells (DCs) play a crucial role in the uptake and processing of antigens found within the respiratory tract. Respiratory syncytial virus (RSV) is a common respiratory tract pathogen in children that induces an influx of DCs to the mucosal surfaces of the lung. Using a neonatal lamb model, we examined the in vivo permissiveness of DCs to RSV infection, as well as overall cell surface changes and cytokine responses of isolated lung DCs after bovine RSV (BRSV) infection. We report that isolated lung DCs and alveolar macrophages ...


Enhanced Surfactant Protein And Defensin Mrna Levels And Reduced Viral Replication During Parainfluenza Virus Type 3 Pneumonia In Neonatal Lambs, Branka Grubor, Jack Gallup, David Meyerholz, Erika Crouch, Richard Evans, Kim Brogden, Howard Lehmkuhl, Mark Ackermann 2016 Iowa State University

Enhanced Surfactant Protein And Defensin Mrna Levels And Reduced Viral Replication During Parainfluenza Virus Type 3 Pneumonia In Neonatal Lambs, Branka Grubor, Jack Gallup, David Meyerholz, Erika Crouch, Richard Evans, Kim Brogden, Howard Lehmkuhl, Mark Ackermann

Jack M Gallup

Defensins and surfactant protein A (SP-A) and SP-D are antimicrobial components of the pulmonary innate immune system. The purpose of this study was to determine the extent to which parainfluenza type 3 virus infection in neonatal lambs alters expression of sheep beta-defensin 1 (SBD-1), SP-A, and SP-D, all of which are constitutively transcribed by respiratory epithelia. Parainfluenza type 3 viral antigen was detected by immunohistochemistry (IHC) in the bronchioles of all infected lambs 3 days postinoculation and at diminished levels 6 days postinoculation, but it was absent 17 days postinoculation. At all times postinoculation, lung homogenates from parainfluenza type 3 ...


Spud Qpcr Assay Confirms Prexcel-Q Software's Ability To Avoid Qpcr Inhibition, Jack Gallup, Fatoumata Sow, Albert Van Geelen, Mark Ackermann 2016 Iowa State University

Spud Qpcr Assay Confirms Prexcel-Q Software's Ability To Avoid Qpcr Inhibition, Jack Gallup, Fatoumata Sow, Albert Van Geelen, Mark Ackermann

Jack M Gallup

Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends. When removal of inhibitory substances by column or reagent-based methods fails or is incomplete, the remaining option of appropriately, precisely and differentially diluting samples and standards to non-inhibitory concentrations is often avoided due to the logistic problem it poses. To address this, we invented the PREXCEL-Q software program to automate the process of calculating the non-inhibitory dilutions for all ...


In Situ Expression Of Intercellular Adhesion Molecule-1 (Icam-1) Mrna In Calves With Acute Pasteurella Haemolytica Pneumonia, Zaher Radi, Karen Register, E.-K. Lee, Marcus Kehrli, Kim Brogden, Jack Gallup, Mark Ackermann 2016 Iowa State University

In Situ Expression Of Intercellular Adhesion Molecule-1 (Icam-1) Mrna In Calves With Acute Pasteurella Haemolytica Pneumonia, Zaher Radi, Karen Register, E.-K. Lee, Marcus Kehrli, Kim Brogden, Jack Gallup, Mark Ackermann

Jack M Gallup

The in situ expression of intercellular adhesion molecule-1 (ICAM-1) mRNA in normal and pneumonic lung tissues of Holstein calves with bovine leukocyte adhesion deficiency (BLAD) was compared with that of age-matched non-BLAD Holstein calves by in situ hybridization. Twenty-four Holstein calves (both BLAD and non-BLAD) were randomly assigned to one of two experimental groups and inoculated intrabronchially with Pasteurella haemolytica or pyrogen-free saline. Lung tissues were collected and fixed in 10% neutral formalin at 2 or 4 hours postinoculation (PI). The expression and distribution of ICAM-1 mRNA in the different cell types of the lung tissue was detected by in ...


Effects Of Formalin-Inactivated Respiratory Syncytial Virus (Fi-Rsv) In The Perinatal Lamb Model Of Rsv, Rachel Derscheid, Jack Gallup, Cory Knudson, Steven Varga, Drew Grosz, Albert Van Geelen, Shannon Hostetter, Mark Ackermann 2016 Iowa State University

Effects Of Formalin-Inactivated Respiratory Syncytial Virus (Fi-Rsv) In The Perinatal Lamb Model Of Rsv, Rachel Derscheid, Jack Gallup, Cory Knudson, Steven Varga, Drew Grosz, Albert Van Geelen, Shannon Hostetter, Mark Ackermann

Jack M Gallup

Respiratory syncytial virus (RSV) is the most frequent cause of bronchiolitis in infants and children worldwide. There are currently no licensed vaccines or effective antivirals. The lack of a vaccine is partly due to increased caution following the aftermath of a failed clinical trial of a formalin-inactivated RSV vaccine (FI-RSV) conducted in the 1960’s that led to enhanced disease, necessitating hospitalization of 80% of vaccine recipients and resulting in two fatalities. Perinatal lamb lungs are similar in size, structure and physiology to those of human infants and are susceptible to human strains of RSV that induce similar lesions as ...


Monocytic/Macrophagic Pneumonitis After Intrabronchial Deposition Of Vascular Endothelial Growth Factor In Neonatal Lambs, David Meyerholz, Branka Grubor, Tatjana Lazic, Jack Gallup, Marcia De Macedo, Paul McCray, Mark Ackermann 2016 University of Iowa

Monocytic/Macrophagic Pneumonitis After Intrabronchial Deposition Of Vascular Endothelial Growth Factor In Neonatal Lambs, David Meyerholz, Branka Grubor, Tatjana Lazic, Jack Gallup, Marcia De Macedo, Paul Mccray, Mark Ackermann

Jack M Gallup

Preterm and young neonates are prone to inadequate surfactant production and are susceptible to respiratory distress syndrome characterized by alveolar damage and hyaline-membrane formation. Glucocorticoid therapy is commonly used in preterm and young infants to enhance lung maturation and surfactant synthesis. Recently, vascular endothelial growth factor (VEGF) was suggested to be a novel therapeutic agent for lung maturation that lacked adverse effects in mice. The purpose of this study was to assess the safety of incremental concentration (0.0005, 0.005, and 0.05 mg/ml) and duration (16, 24, and 32 hours) of recombinant human VEGF after bronchoscopic instillation ...


Depletion Of Alveolar Glycogen Corresponds With Immunohistochemical Development Of Cd208 Antigen Expression In Perinatal Lamb Lung, David Meyerholz, James DeGraaf, Jack Gallup, Alicia Olivier, Mark Ackermann 2016 University of Iowa

Depletion Of Alveolar Glycogen Corresponds With Immunohistochemical Development Of Cd208 Antigen Expression In Perinatal Lamb Lung, David Meyerholz, James Degraaf, Jack Gallup, Alicia Olivier, Mark Ackermann

Jack M Gallup

CD208 DC lysosomal-associated protein is a marker of activated human dendritic cells; however, recently it was described as a marker of adult type II pneumocytes in many species including humans and sheep. Our hypothesis was that CD208 is developmentally regulated in lung pneumocytes. Lamb lungs at varying stages of development were stained immunohistochemically for CD208 and with Nile red (a fluorescent stain for lamellar bodies of type II cells) along with pulmonary markers of maturation (glycogen stores and surfactant protein A [SP-A] expression) or proliferation (Ki-67). CD208 staining and Nile red were localized to rare pneumocytes in young fetal lambs ...


Sucrose Stabilization Of Respiratory Syncytial Virus (Rsv) During Nebulization And Experimental Infection, Drew Grosz, Albert van Geelen, Jack Gallup, Shannon Hostetter, Rachel Derscheid, Mark Ackermann 2016 Iowa State University

Sucrose Stabilization Of Respiratory Syncytial Virus (Rsv) During Nebulization And Experimental Infection, Drew Grosz, Albert Van Geelen, Jack Gallup, Shannon Hostetter, Rachel Derscheid, Mark Ackermann

Jack M Gallup

Background Respiratory syncytial virus (RSV) is a common respiratory pathogen that can cause severe pneumonia. In vivo studies of RSV can be difficult due to variation in viral infection and disease severity in some animal models. Factors that may contribute to the variation are decreases in viral titer due to preparation and storage and method of virus administration. Nebulization is one method of RSV administration that provides even distribution of virus to all lung lobes; however, the exact quantity of the virus killed by nebulization is not defined. To test the hypothesis that sucrose enhances RSV stability and infectivity, a ...


Reduced Clearance Of Respiratory Syncytial Virus Infection In A Preterm Lamb Model, David Meyerholz, Branka Grubor, Sasha Fach, Randy Sacco, Howard Lehmkuhl, Jack Gallup, Mark Ackermann 2016 Iowa State University

Reduced Clearance Of Respiratory Syncytial Virus Infection In A Preterm Lamb Model, David Meyerholz, Branka Grubor, Sasha Fach, Randy Sacco, Howard Lehmkuhl, Jack Gallup, Mark Ackermann

Jack M Gallup

Respiratory syncytial virus (RSV) causes significant respiratory disease in children worldwide. For the study of severe RSV disease seen in preterm infants, a suitable animal model is lacking. The novel hypothesis of this study was that preterm lambs are susceptible to bovine RSV (bRSV) infection, an analogous pneumovirus with ruminant host specificity, and that there would be age-dependent differences in select RSV disease parameters. During RSV infection, preterm lambs had elevated temperatures and respiration rates with mild anorexia and cough compared to controls. Gross lesions included multifocal consolidation and atelectasis with foci of hyperinflation. Microscopic lesions included multifocal alveolar septal ...


Development Of An In Vivo Rnai Protocol To Investigate Gene Function In The Filarial Nematode, Brugia Malayi, Chuanzhe Song, Jack Gallup, Timothy Day, Lyric Bartholomay, Michael Kimber 2016 Iowa State University

Development Of An In Vivo Rnai Protocol To Investigate Gene Function In The Filarial Nematode, Brugia Malayi, Chuanzhe Song, Jack Gallup, Timothy Day, Lyric Bartholomay, Michael Kimber

Jack M Gallup

Our ability to control diseases caused by parasitic nematodes is constrained by a limited portfolio of effective drugs and a paucity of robust tools to investigate parasitic nematode biology. RNA interference (RNAi) is a reverse-genetics tool with great potential to identify novel drug targets and interrogate parasite gene function, but present RNAi protocols for parasitic nematodes, which remove the parasite from the host and execute RNAi in vitro, are unreliable and inconsistent. We have established an alternative in vivo RNAi protocol targeting the filarial nematode Brugia malayi as it develops in an intermediate host, the mosquito Aedes aegypti. Injection of ...


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