University of Nebraska-Lincoln
Langston University
University of Tennessee, Knoxville
University of Arkansas, Fayetteville
Department of Primary Industries and Regional Development, Western Australia
Utah State University
Foliar Expression Of Candidate Genes Involved In Condensed Tannin Biosynthesis In White Clover (Trifolium Repens),
2023
Molecular Plant Breeding Cooperative Research Centre, Australia
Foliar Expression Of Candidate Genes Involved In Condensed Tannin Biosynthesis In White Clover (Trifolium Repens), Stephen N. Panter, J. Simmonds, A. Winkworth, A. Mouradov, G. C. Spangenberg
International Grassland Congress Proceedings
Bloat disease in cattle and sheep is caused by the rapid microbial degradation of protein-rich fodder in the rumen. This leads to the production of protein foams that trap gases, causing bloat, a condition that is often fatal to livestock and costly to farmers. Condensed tannins (CTs) are phenolic polymers produced by the phenylpropanoid pathway of plants (Figure 1). CTs bind to proteins under acidic to neutral conditions, such as those present in the rumen, slowing their breakdown. A diet with a CT content of between 2% and 4% by dry weight, which is provided by some pasture legumes (e.g. …
Discovery, Isolation And Characterisation Of Promoters In White Clover (Trifolium Repens),
2023
La Trobe University, Australia
Discovery, Isolation And Characterisation Of Promoters In White Clover (Trifolium Repens), C. M. Labandera, Y. H. Lin, E. Ludlow, M. Emmerling, U. John, P. W. Sale, C. Pallaghy, G. C. Spangenberg
International Grassland Congress Proceedings
The availability of a suite of promoters with a range of spatial, temporal and inducible expression patterns is of significant importance to control targeted expression of genes for molecular breeding in forage species. A range of resources and tools have been developed for promoter isolation and characterisation in white clover (Trifolium repens L.), including a comprehensive BAC library and a 15K unigene microarray.
Novel Genotypes Of The Subtropical Grass Eragrostis Curvula For The Analysis Of Apomixis (Diplospory),
2023
Universidad de Buenos Aires, Argentina
Novel Genotypes Of The Subtropical Grass Eragrostis Curvula For The Analysis Of Apomixis (Diplospory), S. Cardone, P. Polci, J. P. Selva, M. Mecchia, S. C. Pessino, P. Voigt, G. C. Spangenberg, V. Echenique
International Grassland Congress Proceedings
Eragrostis curvula (Schrad.) Nees is a variable grass native to Southern Africa. Its several forms, known as lovegrasses, were introduced to Australia, USA and Argentina as forage perennial grasses. Apomixis is a common trait in the genus Eragrostis, with diplospory being the most frequent type. Sexual reproduction also occurs in Eragrostis, although not frequently. Since most tetraploid Eragrostis lines are apomictic, the generation of a sexual tetraploid strain is a requirement for linkage analysis of the gene(s) governing the apomictic character. Furthermore, isogenic lines of the same ploidy, reproducing alternatively by sexuality or apomixes, represent an ideal system …
Isolation And Characterisation Of Genes Encoding Malate Synthesis And Transport Determinants In The Aluminum-Tolerant Australian Weeping-Grass (Microlaena Stipoides),
2023
La Trobe University, Australia
Isolation And Characterisation Of Genes Encoding Malate Synthesis And Transport Determinants In The Aluminum-Tolerant Australian Weeping-Grass (Microlaena Stipoides), R. M. Polotnianka, E. Ribarev, L. Mackin, K. A. Sivakumaran, G. D. Nugent, U. P. John, G. C. Spangenberg
International Grassland Congress Proceedings
Acid soils cover some 40% of the Earth’s arable land where they represent a major limitation to plant production. Plant growth on acid soils is primarily limited due to aluminium (Al) solubilized by acidity into toxic Al3+ cations which will inhibit root growth resulting in poor uptake of water and nutrients. Many important pasture species lack sufficient Al tolerance within their germplasm to allow effective breeding for this character.
Development And Use Of A Tool For Automated Alignments Of Genes In The Rice Bac's Genbank Card Against Other Species,
2023
INRA, France
Development And Use Of A Tool For Automated Alignments Of Genes In The Rice Bac's Genbank Card Against Other Species, P. Barre, G. Darrieutort, J. Auzanneau, B. Julier
International Grassland Congress Proceedings
In many cases, the analysis of the genetic bases of any trait requires molecular markers and if possible co-dominant PCR-based ones. In perennial fodder species, the number of publicly available markers (microsatellites and Sequence Tagged Site (STS)) is limited. Our goal is to use sequences from model grass species, i.e. rice, wheat, maize, barley, in L. perenne in order to develop STS markers in interesting regions such as under a QTL (Quantitative Trait Loci) or around a candidate gene,. As the genome sequence of rice is now available, the objective was to use the sequences of genes included in the …
Screening Of Perennial Grasses And A Mutant Maize Collection By Fourier-Transformed Infrared (Ftir) Spectroscopy For Improved Biofuel Traits,
2023
Institute of Grassland & Environmental Research, UK
Screening Of Perennial Grasses And A Mutant Maize Collection By Fourier-Transformed Infrared (Ftir) Spectroscopy For Improved Biofuel Traits, S. C. Thain, P. Morris, S. Hawkins, C. Morris, I. S. Donnison
International Grassland Congress Proceedings
Currently the potential of biomass crops, including grasses, is limited because most species have not been bred for this purpose. However traits such as lignification, phenolic cross-linking and carbohydrate accessibility, which are also important for nutritive quality in forage grasses, can affect potential biofuel quality in applications such as combustion, fast-pyrolysis or fermentation. A collection of Lolium and Festuca species known to exhibit a range of lignin, cell wall phenolic and carbohydrate concentrations have been used to test optimum characteristics for biofuel processing. This collection formed a “calibration” set for subsequent high through-put FTIR chemical screening of additional plant lines: …
Application Of Molecular Markers Derived From Medicago Truncatula In White Clover (Trifolium Repens L.),
2023
Institute of Grassland and Environmental Research, UK
Application Of Molecular Markers Derived From Medicago Truncatula In White Clover (Trifolium Repens L.), C. Jones, M. T. Abberton
International Grassland Congress Proceedings
White clover is the major forage legume of temperate areas. Genome maps have been produced recently (Jones et al., 2003; Barrett et al., 2004) and the location of QTL for important agricultural traits reported (Abberton et al., 2004). White clover is closely related to the model legume Medicago truncatula and there is likely to be considerable benefit in applying genomic resources from model to crop. However, the extent of synteny between the species must be established. Here we present preliminary results detailing progress towards this goal.
A Proposal For An International Transcriptome Initiative For Forage And Turf: Microarray Tools For Expression Profiling In Ryegrass, Clover And Grass Endophytes,
2023
La Trobe University, Australia
A Proposal For An International Transcriptome Initiative For Forage And Turf: Microarray Tools For Expression Profiling In Ryegrass, Clover And Grass Endophytes, T. Webster, N. Nguyen, C. Rhodes, S. A. Felitti, R. Chapman, D. Edwards, G. C. Spangenberg
International Grassland Congress Proceedings
Knowledge of the expression pattern of genes provides a valuable insight into gene function and role in determining the observed heritable phenotype. High–density cDNA and oligonucleotide microarrays represent powerful tools for transcriptome analysis to gain an understanding of gene expression patterns for thousands of genes. Internationally coordinated efforts in transcriptome analyses and sharing of microarray resources will benefit the advancement of our understanding of gene function in forage and turf species.
Screening Genes For Association With Loci For Nitrogen-Use Efficiency In Perennial Ryegrass By PyrosequencingTm,
2023
Plant Research International B.V., The Netherlands
Screening Genes For Association With Loci For Nitrogen-Use Efficiency In Perennial Ryegrass By PyrosequencingTm, O. Dolstra, D. Dees, J. -D. Driesprong, E. N. Van Loo
International Grassland Congress Proceedings
The application of marker-assisted selection to improve quantitative traits in perennial ryegrass (Lolium perenne) is cumbersome. It requires a priori knowledge on the association of markers and genes. The knowledge on the chromosomal location of major genes for quantitative traits as well as on gene sequences is rapidly growing. However, determination of the genetic constitution of parents prior to their use in breeding still is impractical. More realistic is to collect association data along with the testing activities needed for breeding new varieties. This study uses changes in allele frequency due to selection as a criterion for gene-trait …
Gene-Associated Single Nucleotide Polymorphism (Snp) Discovery In Perennial Ryegrass (Lolium Perenne L.),
2023
La Trobe University, Australia
Gene-Associated Single Nucleotide Polymorphism (Snp) Discovery In Perennial Ryegrass (Lolium Perenne L.), J. W. Forster, N. O. I. Cogan, A. C. Vecchies, R. C. Ponting, M. C. Drayton, J. George, J. L. Dumsday, G. C. Spangenberg, K. F. Smith
International Grassland Congress Proceedings
Perennial ryegrass (Lolium perenne L.) is the most important grass species for temperate pasture systems world-wide. Varietal improvement programs for this obligate outbreeding species are based on polycrossing of multiple parents to produce heterogeneous synthetic populations. The complexity of breeding systems creates challenges and opportunities for molecular marker technology development and implementation. Previous research has led to: the generation of a comprehensive suite of simple sequence repeat (SSR) markers, reference genetic map construction, comparative genetic studies, QTL identification, and population structure analysis. Emphasis has now shifted from the use of anonymous genetic markers linked to trait-specific genes to the …
Isolation Of Candidate Genes Involved In Cold Temperatures Response In Festuca Pratensis Huds., Using Suppression Subtractive Hybridisation And Microarray Approaches,
2023
Agricultural University of Norway, Norway
Isolation Of Candidate Genes Involved In Cold Temperatures Response In Festuca Pratensis Huds., Using Suppression Subtractive Hybridisation And Microarray Approaches, H. Rudi, V. Alm, L. Opseth, A. Larsen, O. A. Rognli
International Grassland Congress Proceedings
The objective of this work was to isolate candidate genes which are differentially expressed following cold-acclimation and develop SNPs to test for associations between candidate genes and frost tolerance. The ability to develop sufficient levels of tolerance against freezing temperatures through cold-acclimation (hardening) is crucial for survival of grasses and winter cereals in temperate climate. Meadow fescue (Festauca pratensis Huds.) is one of the most important forage grass species in Northern Europe. The preference of Festuca instead of Lolium in Norway is due to its superior combination of winter hardiness and forage quality.
Ryegrass Astra: A Web-Based Resource For Lolium Est Analysis,
2023
La Trobe University, Australia
Ryegrass Astra: A Web-Based Resource For Lolium Est Analysis, G. C. Spangenberg, T. Sawbridge, E. K. Ong, C. G. Love, T. A. Erwin, E. G. Logan, D. Edwards
International Grassland Congress Proceedings
Perennial ryegrass (Lolium perenne L.) is a major grass species of temperate pastoral agriculture.
Positive Effect Of Increased Aflp Diversity Among Parental Plants On Yield Of Polycross Progenies In Perennial Ryegrass (Lolium Perenne L.),
2023
Swiss Federal Research Station for Agroecology and Agriculture, Switzerland
Positive Effect Of Increased Aflp Diversity Among Parental Plants On Yield Of Polycross Progenies In Perennial Ryegrass (Lolium Perenne L.), R. Kölliker, B. Boller, F. Widmer
International Grassland Congress Proceedings
In outbreeding forage crops such as perennial ryegrass (Lolium perenne L.), genetic diversity among parental plants may greatly influence the success of a cultivar through mechanisms such as heterosis and inbreeding depression. The aim of this study was to evaluate the use of amplified fragment length polymorphism (AFLP) markers to select polycross parents with contrasting levels of genetic diversity and to analyse genetic diversity and agronomic performance of first and second generation synthetic progenies (Syn1, Syn2).
Genomic Constitution Of Festulolium Varieties,
2023
Institute of Experimental Botany, Czech Republic
Genomic Constitution Of Festulolium Varieties, D. Kopecky, V. Cernoch, R. Capka, J. Dolezel
International Grassland Congress Proceedings
Hybrids between species of ryegrass (Lolium) and fescue (Festuca) combine useful agronomical characteristics such as rapid establishment from seed and fodder quality from ryegrass and tolerance against abiotic and biotic stressses from fescue. The superior potential of hybrids has stimulated breeding programs generating so called Festulolium varieties. While the varieties have been evaluated extensively for their agronomic characteristics, little information is publicly available on their genomic constitution. The aim of our study was to analyse genomic constitution of a representative set of commercially available European Festulolium cultivars. To do this, we have employed genomic in situ …
Genetic Changes Over Breeding Generations Of Festulolium,
2023
Institut National de la Recherche Agronomique, France
Genetic Changes Over Breeding Generations Of Festulolium, M. Ghesquière, P. Barre, L. Barrot
International Grassland Congress Proceedings
Festulolium hybrids are a valuable breeding source for tolerance to abiotic stress and to make grass more persistent under drought and in cold environments. In 2004, the EU Commission enlarged the definition of Festulolium which may now include all hybrids between Lolium sp. and Festuca sp. and not only those between L. multiflorum and F. pratensis. We here report allele frequencies at two unlinked PCR-based marker loci in populations derived from tetraploid (2n=4x=28) L. multiflorum x F. glaucescens hybrids where breeding history enables us to test the effects of selection vs that of genetic drift.
Development And Testing Of Novel Chloroplast Markers For Perennial Ryegrass From De Novo Sequencing And In Silico Sequences,
2023
Teagasc, Ireland
Development And Testing Of Novel Chloroplast Markers For Perennial Ryegrass From De Novo Sequencing And In Silico Sequences, S. Mcgrath, T. R. Hodkinson, S. Barth
International Grassland Congress Proceedings
Chloroplast DNA is uniparentally inherited and non-recombinant in Lolium perenne. These properties make the chloroplast genome a useful tool for studying inter- and intra- specific relationships. Previous genetic studies on L. perenne have used chloroplast sequence data. However, the relative lack of variation in the chloroplast genome limits its usefulness for analysis at the single individual level within a species. However, chloroplast SSR markers have recently been shown to have high levels of polymorphism (Provan et al., 2004). This is the first study to design and employ such markers for L. perenne. The objectives of this study …
Genetic Analysis Of The Interaction Between Perennial Ryegrass And The Fungal Endophyte Neotyphodium Lolii,
2023
Molecular Plant Breeding Cooperative Research Centre, Australia
Genetic Analysis Of The Interaction Between Perennial Ryegrass And The Fungal Endophyte Neotyphodium Lolii, E. Van Zijll De Jong, A. C. Vecchies, M. P. Dobrowolski, N. O. I. Cogan, K. F. Smith, G. C. Spangenberg, J. W. Forster
International Grassland Congress Proceedings
The fungal endophyte Neotyphodium lolii is widely distributed in perennial ryegrass pastures, especially in Australia and New Zealand. The presence of the endophyte is associated with improved tolerance to water and nutrient stress and resistance to insect pests, but is accompanied by reduced herbivore feeding. The molecular mechanisms responsible for these endophyte-related traits are in general poorly understood. Comparisons of different grass-endophyte associations show that endophyte-related traits are affected by both endophyte and host genotype, and environmental interactions.
Microarray-Based Transcriptome Analysis Of The Interaction Between Perrenial Ryegrass (Lolium Perenne) And The Fungal Endophyte Neotyphodium Lolii,
2023
La Trobe University, Australia
Microarray-Based Transcriptome Analysis Of The Interaction Between Perrenial Ryegrass (Lolium Perenne) And The Fungal Endophyte Neotyphodium Lolii, S. Felitti, P. Tian, T. Webster, D. Edwards, G. C. Spangenberg
International Grassland Congress Proceedings
Neotyphodium lolii, Neotyphodium coenophialum and Epichloë festucae are common symbiotic fungal endophytes of the temperate pasture grasses perennial ryegrass (Lolium perenne), tall fescue (Festuca arundinacea) and red fescue (Festuca rubra), respectively. A genomic resource of 13,964 expressed sequence tags (ESTs), representing 7,585 unique endophyte genes, has been established for Neotyphodium and Epichloë fungal endophytes.
A High-Throughput Gene Silencing Approach For Studying The Interaction Between Perennial Ryegrass (Lolium Perenne) And The Fungal Endophyte Neotyphodium Lolii,
2023
La Trobe University, Australia
A High-Throughput Gene Silencing Approach For Studying The Interaction Between Perennial Ryegrass (Lolium Perenne) And The Fungal Endophyte Neotyphodium Lolii, S. Felitti, P. Tian, D. Edwards, G. C. Spangenberg
International Grassland Congress Proceedings
Perennial ryegrass (Lolium perenne L.) and its fungal endophyte (Neotyphodium lolii) are known to establish a mutualistic association that impacts on the agronomic productivity of endophyte-infected ryegrass pastures. To study this interaction at the molecular level, a genomic resource consisting of 13,964 endophyte ESTs has been generated. However, the functions of a large proportion of these genes remain to be elucidated. Recent work has demonstrated the potential for RNA-mediated gene silencing to suppress gene expression in a sequence specific manner thus allowing for the subsequent analysis of gene function.
Isolation And Characterization Of Novel Btb Domain Protein Encoding Genes From Fungal Grass Endophytes,
2023
La Trobe University, Australia
Isolation And Characterization Of Novel Btb Domain Protein Encoding Genes From Fungal Grass Endophytes, M. Ramsperger, S. A. Felitti, D. Edwards, G. C. Spangenberg
International Grassland Congress Proceedings
Pasture grasses belonging to the Pooideae sub-family of the Poaceae family frequently host symbiotic fungal endophytes. These include the sexual Epichloë species and the anamorphic asexual Neotyphodium species, which are thought to have evolved from Epichloë species either by the direct loss of sexual reproduction or by interspecific hybridisation. The two key temperate pasture grasses, tall fescue (Festuca arundinacea Schreb) and perennial ryegrass (Lolium perenne L.) interact with the fungal endophytes N. coenophialum and N. lolii, respectively. Large insert genomic DNA libraries are valuable resources for the discovery and isolation of genes and their regulatory sequences, for …
