Other Plant Sciences Commons^™

Increased Light Exposure Decreases Lichen Abundance On Linden Trees In The Prairie Grassland Biome Of West Central Minnesota, Cameron Berthiaume, Sanjana Kidambi, Madelyn Schoenberger

Scholarly Horizons: University of Minnesota, Morris Undergraduate Journal

Percent lichen cover on the four cardinal faces of linden trees on the University of Minnesota Morris campus was recorded in order to determine if lichen abundance is linked to the varying levels of sunlight each cardinal face receives.

Pearl Millet Response To Drought: A Review, Nikee Shrestha, Hao Hu, Kumar Shrestha, Andrew N. Doust

Agronomy & Horticulture -- Faculty Publications

The C4 grass pearl millet is one of the most drought tolerant cereals and is primarily grown in marginal areas where annual rainfall is low and intermittent. It was domesticated in sub-Saharan Africa, and several studies have found that it uses a combination of morphological and physiological traits to successfully resist drought. This review explores the short term and long-term responses of pearl millet that enables it to either tolerate, avoid, escape, or recover from drought stress. The response to short term drought reveals fine tuning of osmotic adjustment, stomatal conductance, and ROS scavenging ability, along with ABA and ethylene …

Screening Spring Wheat Genotypes For Tadreb-B1 And Fehw3 Genes Under Severe Drought Stress At The Germination Stage Using Kasp Technology, Elsayed A. Elsayed, Asmaa A. M. Ahmed, Matías Schierenbeck, Schierenbeck Y. Hussein, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Agronomy & Horticulture -- Faculty Publications

Drought stress is a major yield-limiting factor throughout the world in wheat (Triticum aestivum L.), causing losses of up to 80% of the total yield. The identification of factors affecting drought stress tolerance in the seedling stage is especially important to increase adaptation and accelerate the grain yield potential. In the current study, 41 spring wheat genotypes were tested for their tolerance to drought at the germination stage under two different polyethylene glycol concentrations (PEG) of 25% and 30%. For this purpose, twenty seedlings from each genotype were evaluated in triplicate with a randomized complete block design (RCBD) in …

Long-Term Manure Application Improves Soil Health And Stabilizes Carbon In Continuous Maize Production System, Saurav Das, Daniel Liptzin, Bijesh Maharjan

Agronomy & Horticulture -- Faculty Publications

Soil health lies at the core of a sustainable food production system. A comprehensive evaluation of different agronomic practices and their effect on soil health is essential to determine the best practices that support soil ecosystem services. However, it may take years or decades to observe measurable changes in soil health under varying management practices. The objective of this experiment was to evaluate the effects of long-term (>77 years) manure and inorganic nitrogen (N) fertilizer on soil health and determine the interrelationship among the measured soil bio-physicochemical indicators. The study also aims to understand the sustainability of the monocropping …

The Intertwining Of Zn-Finger Motifs And Abiotic Stress Tolerance In Plants: Current Status And Future Prospects, Debojyoti Moulick, Karma Landup Bhutia, Sukamal Sarkar, Anirban Roy, Udit Nandan Mishra, Biswajit Pramanick, Sagar Maitra, Tanmoy Shankar, Swati Hazra, Milan Skalicky, Marian Brestic, Viliam Barek, Akbar Hossain

Agronomy & Horticulture -- Faculty Publications

Environmental stresses such as drought, high salinity, and low temperature can adversely modulate the field crop’s ability by altering the morphological, physiological, and biochemical processes of the plants. It is estimated that about 50% + of the productivity of several crops is limited due to various types of abiotic stresses either presence alone or in combination (s). However, there are two ways plants can survive against these abiotic stresses; a) through management practices and b) through adaptive mechanisms to tolerate plants. These adaptive mechanisms of tolerant plants are mostly linked to their signalling transduction pathway, triggering the action of plant …

Editorial: Genetic Validation And Its Role In Crop Improvement, Ahmed Sallam, Ahmad M. Alqudah, P. Stephen Baenziger, Awais Rasheed

Agronomy & Horticulture -- Faculty Publications

Gene discovery for economically important traits has remained a challenging Frontier in crop genomics and breeding. The recent advances in DNA sequencing technologies and genetic analysis approaches paved the way for discovering many genes and hotspot genomic regions controlling target traits. The detection of novel genomic regions or candidate genes is very useful for plant breeders and geneticists to improve crops, dissect the genetics of complex traits, and understand the biological mechanisms of genes underpinning traits of interest. Quantitative trait loci (QTL) mapping and genome-wide association studies (GWAS) dominated recent crop gene discovery research. These studies are becoming routine activities …

Transformation And Gene Editing In The Bioenergy Grass Miscanthus, Anthony Trieu, Mohammad B. Belaff, Pradeepa Hirannaiah, Shilpa Manjunatha, Rebekah Wood, Yokshitha Bathula, Rebecca L. Billingsley, Anjali Arpan, Erik J. Sacks, Thomas E. Clemente, Stephen P. Moose, Nancy A. Reichert, Kankshita Swaminathan

Agronomy & Horticulture -- Faculty Publications

Background: Miscanthus, a C4 member of Poaceae, is a promising perennial crop for bioenergy, renewable bioproducts, and carbon sequestration. Species of interest include nothospecies M. x giganteus and its parental species M. sacchariforus and M. sinensis. Use of biotechnology-based procedures to genetically improve Miscanthus, to date, have only included plant transformation procedures for introduction of exogenous genes into the host genome at random, non-targeted sites.

Results: We developed gene editing procedures for Miscanthus using CRISPR/Cas9 that enabled the mutation of a specific (targeted) endogenous gene to knock out its function. Classified as paleo-allopolyploids (duplicated ancient …

Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail

Agronomy & Horticulture -- Faculty Publications

Common bunt (caused by Tilletia caries and T. Foetida) is a major wheat disease. It occurs frequently in the USA and Turkey and damages grain yield and quality. Seed treatment with fungicides is an effective method to control this disease. However, using fungicides in organic and low-income fields is forbidden, and planting resistant cultivars are preferred. Due to the highly effective use of fungicides, little effort has been put into breeding resistant genotypes. In addition, the genetic diversity for this trait is low in modern wheat germplasm. Synthetic wheat genotypes were reported as an effective source to increase the …

Inheritance Of 2,4‑Dichlorophenoxyacetic Acid (2,4‑D) Resistance In Amaranthus Palmeri, Chandrima Shyam, Dallas E. Peterson, Amit J. Jhala, Mithila Jugulam

Agronomy & Horticulture -- Faculty Publications

In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F₁ progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F₁ family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F₁ families were used in pairwise crosses to generate pseudo-F₂ families. Segregation (resistance or susceptibility) of progenies from the F₂ families in response to a …

A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace

Agronomy & Horticulture -- Faculty Publications

Average dietary fibre intakes have increased little in the past twenty years in many countries, including the USA¹ . Multi-million-dollar campaigns promoting fruits, vegetables, whole grains and other foods high in fibre have delivered only small changes in diets² , and consumers have not changed from traditional staples to whole-grain options³ . UK millers report that consumption of whole-wheat bread has actually declined over the past decade (P. Shewry, personal communication). In the US, white flour, which is lower in fibre than whole-wheat flour, accounts for nearly 40% of the fibre intake⁴ . We believe that …

Early Detection, Rapid Response Invasives Management: Documenting Perennial Pepperweed In New Hampshire Using Eddmaps, Final Project Report, December 2022, Malin Clyde

UNH Cooperative Extension

No abstract provided.

Genetic Variations Of Narcissus Tazetta And Selected Narcissus Cultivars Based On The Sequence Analysis Of Nrits And TrnL‑Is‑TrnF Regions, Seo Young Park, Mi Jin Jeon, Young Hee Joung, Keenan L. Amundsen, Gianluca Burchi, Angelo Porcelli, Mark S. Roh

Agronomy & Horticulture -- Faculty Publications

Narcissus tazetta var. chinensis (N. taz. var. chi.) accessions collected from the coastal areas of China, Korea, and Japan were compared with accessions of lower ranks of Narcissus tazetta (N. taz.) from Italy to study the genetic variations and with several morphologically similar cultivars available in the trade. Sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrITS) dendrogram and the chloroplast trnL-IS-trnF intergeneric spacer-gene (cpIS) were analyzed by maximum likelihood (ML) and posterior probability using STRUCTURE program. All single fower form of N. taz. var. chi. collected from China, Korea, and …

Evaluation For Using Expended Bioelectrochemical Systems As Soil Amendments For Improved Corn Plant Growth And A Drought Resistant Soil., Jackson Lee Sauers

Theses and Dissertations

A long-held practice is to mix agricultural soil with a soil amendment to improve growing conditions in crops. A common soil amendment is biosolids produced from both municipal and dairy wastewater due to the macro- and micronutrients within it. Both the agricultural and wastewater industries are participating in the Circular Economy concept (CEC). Two experiments explored using expended bioelectrochemical systems (BES) that treated either synthetic dairy wastewater (DWW) or synthetic municipal wastewater (SWW) as soil amendments to improve corn plant growth when treated with three different nutrient treatments: 100%- 50%- and 0% Hoagland Nutrient Solutions. Biochar and used terracotta clay …

Poisson Hurdle Model-Based Method For Clustering Microbiome Features, Zhili Qiao, Elle Barnes, Susannah Tringe, Daniel P. Schachtman, Peng Liu

Agronomy & Horticulture -- Faculty Publications

Motivation: High-throughput sequencing technologies have greatly facilitated microbiome research and have generated a large volume of microbiome data with the potential to answer key questions regarding microbiome assembly, structure and function. Cluster analysis aims to group features that behave similarly across treatments, and such grouping helps to highlight the functional relationships among features and may provide biological insights into microbiome networks. However, clustering microbiome data are challenging due to the sparsity and high dimensionality.

Results: We propose a model-based clustering method based on Poisson hurdle models for sparse microbiome count data. We describe an expectation–maximization algorithm and a …

Deciphering The Genetic Architecture Of Key Female Floral Traits For Hybrid Wheat Seed Production, Juan Jimenez

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Wheat (Triticum aestivum L.) is a staple cereal that provides 20% of the calories and proteins in human intake (Ray et al., 2013). Global population is projected to increase to 9.7 billion by 2050. Food production must increase by 70% to feed this future population. Wheat production is in crisis due to political and environmental challenges and is projected to decline by 0.8% in 2022 (FAO, 2022). To ensure food security yield genetic gain must increase by around 1.4% annually. Taking advantage of heterosis, hybrid wheat has the potential to boost grain yield. However, hybrid wheat seed production systems …

Nitrification Inhibitor, Nitrogen Source, And Herbicide Effects On Soil Nitrogen Transformations And Corn Yield, William Neels

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Nitrogen fertilizer management continue to be challenging due to potential nitrogen losses under variable weather conditions. The objective of this was to evaluate the performance of nitrification inhibitors, nitrogen sources and herbicides on in-season nitrogen availability and agronomy indicators. A two site-year field experiment was conducted in silty clay loam soil in corn phase of the corn-soybean rotation at Central Nebraska. The treatments included three herbicide (no pre-emergence, Acuron, Resicore) and five nitrogen treatments: 1) control, 2) anhydrous ammonia, 3) anhydrous ammonia without nitrification inhibitor, 4) urea with nitrification inhibitors, and 5) urea without nitrification inhibitors. Results indicated that nitrogen …

Addressing Challenges Of Dryland Production Of Sunflowers And Corn In The Semi-Arid High Plains Of Nebraska, Zhan Orazov

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Corn and sunflower are value crops for America. Cultivation of corn and sunflower often vary depending on growing environment. Selecting appropriate planting dates, hybrids and plant density frequently concerns farmers. This concern is understandable because the decision made directly impacts final income. The objectives were to evaluate interaction of corn and sunflower planting dates and hybrid maturity and evaluate interaction of corn flex hybrids and plant density under conditions of western Nebraska. Nine corn hybrids with relative maturity ranging from 86 to 105 days were sown between early May and late June in first and between late April and early …

Using Xbeach To Describe The Performance Of An Intertidal Vegetation Shoreline Stabilization Treatment, Elizabeth Winter

Theses and Dissertations

The purpose of this project is to predict the hydrodynamic and morphodynamics of an engineered vegetation-only shoreline restoration project in Little Lagoon, Alabama under different storm and sea level rise scenarios. Little Lagoon is a shallow, single-inlet lagoon located in Baldwin County, Alabama that has been experiencing shoreline erosion for the past 28 years. A living shoreline using vegetation only (Spartina alterniflora) was implemented in the southwest corner of the lagoon, located within Bon Secour National Wildlife Refuge, to create habitat, improve water quality, and prevent future erosion. This research compares “with-project” and “without-project” hydrodynamics and morphodynamics using XBeach in …

A Glass Bead Semi-Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez, Karin V. Van Dijk

Agronomy & Horticulture -- Faculty Publications

Background: Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudate collection. Here, we present a newly designed semi-hydroponic system that uses glass beads as solid support to simulate soil impedance, which combined with drip irrigation, facilitates growth of healthy maize plants, collection and analysis of root exudates, and phenotyping of the roots with minimal growth disturbance or root damage. Results: This system was used to collect root exudates from seven maize genotypes using water or 1 mM CaCl2, …

Comparative Genome Analysis Of Plant Ascomycete Fungal Pathogens With Different Lifestyles Reveals Distinctive Virulence Strategies, Yansu Wang, Jie Wu, Jiacheng Yan, Ming Guo, Lei Xu, Liping Hou, Quan Zou

Agronomy & Horticulture -- Faculty Publications

Background: Pathogens have evolved diverse lifestyles and adopted pivotal new roles in both natural ecosystems and human environments. However, the molecular mechanisms underlying their adaptation to new lifestyles are obscure. Comparative genomics was adopted to determine distinct strategies of plant ascomycete fungal pathogens with different lifestyles and to elucidate their distinctive virulence strategies. Results: We found that plant ascomycete biotrophs exhibited lower gene gain and loss events and loss of CAZyme-encoding genes involved in plant cell wall degradation and biosynthesis gene clusters for the production of secondary metabolites in the genome. Comparison with the candidate effectome detected distinctive variations between …