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Molecular Characterisation Of Bacterial Wilt Resistance In Lolium Multiflorum Lam., B. Studer, B. Boller, F. Windmer, U. K. Posselt, E. Bauer, R. Kölliker 2023 Swiss Federal Research Station for Agroecology and Agriculture, Switzerland

Molecular Characterisation Of Bacterial Wilt Resistance In Lolium Multiflorum Lam., B. Studer, B. Boller, F. Windmer, U. K. Posselt, E. Bauer, R. Kölliker

International Grassland Congress Proceedings

Italian ryegrass (Lolium multiflorum Lam.), a forage grass of prime importance throughout the world, is adversely affected by the pathogen Xanthomonas translucens pv graminis. Breeding for resistant cultivars is the only practicable means of disease control. However, the inheritance of bacterial wilt resistance is largely unknown. The aim of our research is to elucidate genetic control of bacterial wilt resistance using molecular technologies such as genetic linkage mapping and the analysis of quantitative trait loci (QTL).


Ear Emergence Of Different Grass Species Under Finnish Growing Conditions, M. Niskanen, O. Niemeläinen, L. Jauhiainen 2023 MTT Agrifood Research, Finland

Ear Emergence Of Different Grass Species Under Finnish Growing Conditions, M. Niskanen, O. Niemeläinen, L. Jauhiainen

International Grassland Congress Proceedings

Timothy is the most commonly cultivated grass species in Finland. Swards cultivated for silage of hay are of pure timothy or timothy is the dominant species of the mixture. Successful timing of harvesting of the primary growth is very critical in Northern latitudes where the stand develops very rapidly at daylengths of above 18 hours with a daily mean temperature close to 20 °C. The primary growth has to be harvested within a very short period to obtain a yield of high and uniform quality. Short harvesting periods require high capacity harvest machinery which lead to high costs. If it …


The Effect Of Harvest Management On Forage Production And Self-Reseeding Potential Of Italian Ryegrass (Lolium Multiflorum L.), P. W. Bartholomew, R. D. Williams 2023 USDA

The Effect Of Harvest Management On Forage Production And Self-Reseeding Potential Of Italian Ryegrass (Lolium Multiflorum L.), P. W. Bartholomew, R. D. Williams

International Grassland Congress Proceedings

Italian ryegrass (Lolium multiflorum L.) (IRG) can be managed to produce a seed output sufficient for effective re-establishment, without compromising forage yield, it may provide an alternative to perennial cool-season grasses in the Southern Great Plains of the U.S.A. The reduction in cost of replanting and avoidance of cultivation offered by a self-seeding crop may be particularly useful in low-input production systems. We examined the effect of dates of initial harvest in spring and of partial harvests on forage yield, seed output and re-establishment of Italian ryegrass.


The Influence Of Fertiliser Application To Strip-Sown Grasslands On Herbage Production And Quality, A. Kohoutek, P. Komárek, V. Odstrčilová, P. Nerušil 2023 Research Institute of Crop Production, Czech Republic

The Influence Of Fertiliser Application To Strip-Sown Grasslands On Herbage Production And Quality, A. Kohoutek, P. Komárek, V. Odstrčilová, P. Nerušil

International Grassland Congress Proceedings

To increase the productivity of dairy cattle in the Czech Republic requires an improvement in herbage quality and an increase in the net energy of herbage. The decrease in cattle numbers by 50 % and the expansion of the grassland area both result in a surplus of feedstuffs of low quality. Introduction of strip-seeding of legumes and grasses into grasslands in interaction with fertilisation provides a possible solution to this problem.


Constraints On Dairy Cattle Production From Locally Available Forages In Bangladesh, M. A. S. Khan 2023 Bangladesh Agricultural University, Bangladesh

Constraints On Dairy Cattle Production From Locally Available Forages In Bangladesh, M. A. S. Khan

International Grassland Congress Proceedings

The productivity of milk producing animals in Bangladesh is low because of low individual yield and poor fertility. The reasons for the low productivity are complex but, in order of priority, appear to be (a) the imbalanced nature of the nutrients that arise from the digestion of the forage resources, (b) the incidence of disease/parasitism, and (c) the often harsh climatic circumstances. Thus, the purpose of this study was to find out the practical constraints on dairy cattle production from locally available forages under small holding village conditions of Bangladesh.


Pasture Management In Deer Farms In Mauritius, P. Grimaud, P. Thomas, J. Sauzier 2023 CIRAD, France

Pasture Management In Deer Farms In Mauritius, P. Grimaud, P. Thomas, J. Sauzier

International Grassland Congress Proceedings

Rusa deer (Cervus timorensis russa) were introduced into Mauritius in 1639, and with a population of 65,000 deer now provide the first source of red meat in the island. From June to September, the hunting period, extensive ranches ensure the venison market, while intensive farms provide it for the rest of the year. A pasture survey was carried out over 3 years to advise deer farmers on their sward management.


Discriminating Stay-Green Grasses Using Hyperspectral Imaging And Chemometrics, J. Taylor, B. Moore, J. J. Rowland, H. Thomas, H. J. Ougham 2023 Institute of Grassland and Environmental Research, UK

Discriminating Stay-Green Grasses Using Hyperspectral Imaging And Chemometrics, J. Taylor, B. Moore, J. J. Rowland, H. Thomas, H. J. Ougham

International Grassland Congress Proceedings

Screening of plant collections for traits can be expensive, in terms of the number of plants to be screened, the duration of the plant lifecycle and the required observations. This study describes the application of a non-invasive method, hyperspectral imaging, combined with multivariate analysis, to distinguish between homozygous wild-type (YY) Lolium multiflorum and Lolium multiflorum F2 back cross plants heterozygous for y, a recessive Festuca pratensis stay-green gene (Thomas et al., 1997).


Non-Destructive Assessment Of Quality And Yield For Grass-Breeding, A. G. T. Schut, M. J. J. Pustjens, P. W. Wilkins, J. Meuleman, P. Reyns, A. Lovatt, G. W. A. M. van der Heijden 2023 Plant Research International, The Netherlands

Non-Destructive Assessment Of Quality And Yield For Grass-Breeding, A. G. T. Schut, M. J. J. Pustjens, P. W. Wilkins, J. Meuleman, P. Reyns, A. Lovatt, G. W. A. M. Van Der Heijden

International Grassland Congress Proceedings

Selection of cultivars has, until now, been based mainly on dry matter (DM) yields because of the high costs of sampling and chemical analysis. Imaging spectroscopy could reduce costs by limiting sampling and harvesting of individual plots to reference samples (Schut et al., accepted). In this study, the prediction accuracy of DM yields and chemical composition with imaging spectroscopy is evaluated for cultivar selection purposes.


Root Senescence In Red Clover (Trifolium Pratense L.), K. J. Webb, E. Tuck, S. Heywood 2023 Institute of Grassland and Environmental Research, UK

Root Senescence In Red Clover (Trifolium Pratense L.), K. J. Webb, E. Tuck, S. Heywood

International Grassland Congress Proceedings

Legume root systems form a mosaic of living, ageing and dead roots and nodules. The balance between these stages alters during plant development. Stressful events (drought, temperature change, reduced carbon supply, etc.) disturb the balance (Butler et al., 1959). Effects of root and nodule death on soil structure, composition and leaching and on plant persistency are understood poorly. Plants with differing senescence patterns are useful tools to study these effects. Molecular studies of root senescence need detailed knowledge of the process and timing of root senescence and death. Biochemical and histochemical markers of senescence were used to generate preliminary …


Development Of Simple Sequence Repeat (Ssr) Markers And Their Use To Assess Genetic Diversity In Apomictic Guineagrass (Panicum Maximum Jacq.), M. Ebina, Kaori Kouki, S. Tsuruta, M. Takahara, M. Kobayashi, T. Yamamoto, K. Nakajima, H. Nakagawa 2023 Okinawa Prefectural Livestock Experimental Station, Japan

Development Of Simple Sequence Repeat (Ssr) Markers And Their Use To Assess Genetic Diversity In Apomictic Guineagrass (Panicum Maximum Jacq.), M. Ebina, Kaori Kouki, S. Tsuruta, M. Takahara, M. Kobayashi, T. Yamamoto, K. Nakajima, H. Nakagawa

International Grassland Congress Proceedings

Guineagrass is an important and widely grown tropical forage grass. Despite its importance and increasing popularity, only little is known about its genetic diversity (Ebina et al., 2001). Such information is useful for the selection of diverse parents in breeding programmes. Moreover, no simple sequence repeat (SSR) markers have been reported in any apomixis species. In this study SSR markers were developed and used to investigate genetic diversity in germplasm of apomictic guineagrass.


Construction Of Microsatellite-Enriched Libraries For Tropical Forage Species And Characterization Of The Repetitive Sequences Found In Brachiaria Brizantha, Letícia Jungmann, C. B. do Valle, P. R. Laborda, Rosangela M. S. Resende, Liana Jank, A. P. de Souza 2023 Universidade Esdatual de Campinas, Brazil

Construction Of Microsatellite-Enriched Libraries For Tropical Forage Species And Characterization Of The Repetitive Sequences Found In Brachiaria Brizantha, Letícia Jungmann, C. B. Do Valle, P. R. Laborda, Rosangela M. S. Resende, Liana Jank, A. P. De Souza

International Grassland Congress Proceedings

The Brazilian cattle herd comprises 185 million animals fed with about 177 million hectares of native and cultivated pastures (IBGE, 2002). Of the grass species used for forage in Brazil, the African genus Brachiaria is the most widely planted, followed by Panicum, which also has an African origin. Legumes of the Stylosanthes genus, native to the South America, have emerged in the last few years as potential forage species for use with the grasses. These forage species have been bred at Embrapa Beef Cattle and the breeding programs have shown the need for more genetic information including the use …


Development Of Ssr Markers For Variety Identification In Italian Ryegrass (Lolium Multiflorum Lam.), M. Inoue, N. Yuyama, H. Cai 2023 Forage Crop Research Institute, Japan

Development Of Ssr Markers For Variety Identification In Italian Ryegrass (Lolium Multiflorum Lam.), M. Inoue, N. Yuyama, H. Cai

International Grassland Congress Proceedings

Italian ryegrass (IRG, Lolium multiflorum Lam.) is one of the most important cool-season forage grasses in the world, and is the most widely cultivated annual forage grass in Japan. Simple sequence repeat (SSR) markers have the advantages of being PCR-based, multiallelic and possessing high levels of polymorphism. They are very suitable for variety identification, especially for out-crossing species including IRG. The objective of this study was the development of SSR markers for variety identification in IRG.


Development Of A Microsatellite Library In Lolium Perenne, J. King, I. P. King, D. Thorogood, L. Roberts, K. Skøt, K. Elborough 2023 IGER, UK

Development Of A Microsatellite Library In Lolium Perenne, J. King, I. P. King, D. Thorogood, L. Roberts, K. Skøt, K. Elborough

International Grassland Congress Proceedings

Lolium perenne, as one of the most important forage grasses of temperate regions, combines a number of very useful characteristics, e.g., good seedling establishment, with a low resistance to drought and limited winter hardiness. Trait selection and introgression can be greatly enhanced by the use of molecular markers in a genetic linkage map. The aim of this project was the generation of a genomic microsatellite library which when combined with microsatellites developed from a Genethresher database would give good genome coverage coupled to high levels of marker polymorphism.


Isolation Of Ssr Markers From Zoysiagrass, H. Cai, N. Yuyama, M. Inoue 2023 Forage Crop Research Institute, Japan

Isolation Of Ssr Markers From Zoysiagrass, H. Cai, N. Yuyama, M. Inoue

International Grassland Congress Proceedings

The genus Zoysia consists of 16 species that are naturally distributed on sea coasts and grasslands around the Pacific. In Japan, five species of natural zoysiagrasses have been identified from southern Hokkaido to the southwest islands. Of these, Z. japonica Steud. and Z. matrella Merr. have been utilized extensively as turf in Japan and other countries in East Asia. Linkage maps based on RFLP and AFLP markers have been reported in Zoysia (Yaneshita et al., 1999, Cai et al., 2004). Simple sequence repeat (SSR) markers have the advantages of being PCR-based and multiallelic. They are highly polymorphic compared …


A Medicago Truncatula Population Segregating For Aluminum Tolerance, M. Sledge, B. Narasimhamoorthy, G. Jiang 2023 The Samuel Roberts Noble Foundation, USA

A Medicago Truncatula Population Segregating For Aluminum Tolerance, M. Sledge, B. Narasimhamoorthy, G. Jiang

International Grassland Congress Proceedings

Aluminium (Al) toxicity, manifested in inhibition of root elongation and reduced plant growth, is a major cause of poor crop yields on acid soils, which comprise up to 40% of the world’s arable land. Al toxicity associated with acid soils has been a major obstacle in alfalfa (Medicago sativa) production in the USA, as well as in tropical areas of the world. Recent molecular marker mapping studies indicate that the genomes of M. truncatula and M. sativa are highly similar (Choi et al., 2004). Thus, M. truncatula could be used as a source of genes that could …


Quantitative Trait Locus Analysis Of Morphogenetic And Developmental Traits In An Ssr And Aflp-Based Genetic Map Of White Clover (Trifolium Repens L.), M. T. Abberton, N. O. I. Cogan, K. F. Smith, G. Kearney, A. H. Marshall, A. Williams, T. P. T. Michaelson-Yeates, C. Bowen, E. S. Jones, A. C. Vecchies, J. W. Forster 2023 Institute of Grassland and Environmental Research, UK

Quantitative Trait Locus Analysis Of Morphogenetic And Developmental Traits In An Ssr And Aflp-Based Genetic Map Of White Clover (Trifolium Repens L.), M. T. Abberton, N. O. I. Cogan, K. F. Smith, G. Kearney, A. H. Marshall, A. Williams, T. P. T. Michaelson-Yeates, C. Bowen, E. S. Jones, A. C. Vecchies, J. W. Forster

International Grassland Congress Proceedings

Molecular marker-assisted plant breeding is a key target for the temperate legume pasture crop white clover (Trifolium repens L.). The first genetic linkage map of white clover has been constructed using self-fertile mutants to derive an intercross based fourth and fifth generation inbred parental genotypes (F2[I.4R x I.5J]). The framework map was constructed using simple sequence repeat (TRSSR) and amplified fragment length polymorphism (AFLP) markers. Eighteen linkage groups (LG) corresponding to the anticipated 16 chromosomes of white clover (2n = 4x = 32), with a total map length of 825 cM were derived from a total of …


Genetic Mapping In Tetraploid Alfalfa: Results And Prospects, E. C. Brummer, J. G. Robins, B. Alarcón Zúñiga, D. Luth 2023 Iowa State University

Genetic Mapping In Tetraploid Alfalfa: Results And Prospects, E. C. Brummer, J. G. Robins, B. Alarcón Zúñiga, D. Luth

International Grassland Congress Proceedings

Among the difficulties of improving forages is their perennial nature, which necessarily requires long selection cycles to fully evaluate genotypes. Further, traits of particular importance—yield and winter hardiness—are difficult to assess on single plants, necessitating evaluation of progeny, which is both time consuming and expensive. Because of this, yield of many forages, and particularly alfalfa, has not improved substantially over the past 25 years (Riday and Brummer, 2002). Winter hardiness often has a negative correlation with autumn growth, although some evidence suggests this is not always true (Brummer et al., 2000). One way to overcome some of these limitations …


A Glucanase Gene Cosegregates With A Qtl For Crown Rust Resistance In L. Perenne, H. Muylle, J. Baert, E. Van Bockstaele, I. Roldán-Ruiz 2023 Centre of Agricultural Research, Belgium

A Glucanase Gene Cosegregates With A Qtl For Crown Rust Resistance In L. Perenne, H. Muylle, J. Baert, E. Van Bockstaele, I. Roldán-Ruiz

International Grassland Congress Proceedings

An important disease in Lolium spp. is crown rust caused by the fungal pathogen Puccinia coronata. In order to study the genetic background of crown rust resistance in L. perenne, a mapping study was carried out and is discussed below. To identify genomic regions or genes involved in resistance, STS markers are extremely useful. This candidate gene approach was applied in the present study.


Qtl Analysis Of Mineral Content And Grass Tetany Potential In Leymus Wildryes, S. R. Larson, H. F. Mayland 2023 Utah State University

Qtl Analysis Of Mineral Content And Grass Tetany Potential In Leymus Wildryes, S. R. Larson, H. F. Mayland

International Grassland Congress Proceedings

Grass tetany is a metabolic ailment in ruminants, occurring when animals graze rapidly growing C3 grasses with a K/(Mg+Ca) ratio (KRAT) greater than 2.2. High KRAT values have been documented in several forage grasses including diploid Russian wildrye (Jefferson et al., 2001). The objective of this experiment was to identify quantitative trait loci (QTLs) controlling KRAT in allotetraploid wildryes.


Crown Rust Resistance Of Italian Ryegrass Cultivar ‘Axis’ To An Isolate From Japan, T. Kiyoshi, M. Hirata, T. Takamizo, H. Sato, Y. Mano, M. Fujimori 2023 National Institute of Livestock and Grassland Science, Japan

Crown Rust Resistance Of Italian Ryegrass Cultivar ‘Axis’ To An Isolate From Japan, T. Kiyoshi, M. Hirata, T. Takamizo, H. Sato, Y. Mano, M. Fujimori

International Grassland Congress Proceedings

Crown rust (Puccinia coronata) is one the most serious diseases of Italian ryegrass. Crown rust resistance genes in Italian ryegrass have been identified from 'Yamaiku130' and ‘Harukaze’. The aim of this study was to identify novel major resistance genes for gene pyramiding in order to develop cultivars with high levels of durable resistance to crown rust


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