Plant Breeding and Genetics Commons^™

Epigenetics Of Stress Adaptation In Arabidopsis: The Case Of Histone Modifications, Behnaz Najafi Majdabadi Farahani

University of Western Ontario - Electronic Thesis and Dissertation Repository

Changes in the epigenetic status of plants, which contribute to changes in gene expression, in response to abiotic stress are well documented. However, their stability and transmission to subsequent generations, and their incorporation into plant stress adaptation are still a matter for debate. Using chromatin immuno-precipitation and Next Generation Sequencing (ChIP-seq), we compared genome wide enrichment of two histone marks, H3K9ac and H3K4me2, in the progeny of salt stressed and control Arabidopsis plants. Data showed less enrichment of the H3K9ac in the chromatin of the progeny of salt stressed plants, but no changes were detected in the enrichment of the ...

Detection Of Soybean Amino Acid Qtls And Seed Yield Qtls Using Selective Genotyping, Benjamin David Fallen

Doctoral Dissertations

The U.S. Census Bureau projects the world’s population will top more than nine billion by 2050. Today, soybeans account for 56 % of the world oilseed production and 68 % of the world protein meal consumption, with U.S. soybean production accounting for 33 % of the world soybean production. So, to meet the demand of the world’s growing population and of the livestock industry improvements in both the composition and the yield of soybean is essential.

The primary objective of this project was to use molecular markers to identify genomic regions associated with amino acid composition and yield in ...

Cucumber Variety Trials 2012, Rebecca Brown

Rhode Island Agricultural Experiment Station Bulletin

Slicing and pickling cucumber variety trials conducted at Kingston, RI, during the summer of 2012.

Leek Variety Trials, 2012, Rebecca Brown, Noah Leclaire-Conway

Rhode Island Agricultural Experiment Station Bulletin

Variety trial of leek varieties conducted at Kingston, RI, in 2012.

Melon Variety Trials, 2012, Rebecca Brown

Rhode Island Agricultural Experiment Station Bulletin

Melon variety trial conducted at Kingston, RI, in summer of 2012.

Onion Variety Trials, 2012, Rebecca Brown, Noah Leclaire-Conway

Rhode Island Agricultural Experiment Station Bulletin

No abstract provided.

Heritability And Genetic Gain Of Seed Protein, Oil, And Yield Among Ril Of Soybean, Benjamin T. Wiggins

Masters Theses

Soybean is a remarkable crop that is used in many aspects of our everyday lives. The seed protein is used primarily for livestock feed, though it is becoming more available as a meat substitute for human consumption. Soybean oil is used in many products such as plastics, foods, and industrial products. Seed yield is the major trait that is selected for in a breeding program. A population of 239 F₄ derived RIL from the cross of USG 5601T × U99-310255 was used to calculate heritability estimates by parent offspring regression and on an entry mean basis. This population was grown ...

Functional Characterization Of Two Putative Nucleobase Transporters In Arabidopsis Using Heterologous Complementation In Yeast, Sara E. Miller

Master's Theses

To identify the substrate profile function for AtNAT5, one of the twelve members of the NAT/NCS2 gene family putatively identified to transport xanthine and/or uric acid, and to identify other functional aspects of transport for the already characterized AtNCS1 adenine-guanine-uracil transporter (Mourad et al., 2012) we used heterologous complementation techniques in S. cerevisiae mutant strains deficient in nucleobase transport. The ability of transformed yeast cells expressing the AtNAT5 locus to grow in the presence of a panel of nucleobase toxic analogs revealed that the AtNAT5 protein is unable to transport adenine, guanine, cytosine, or uracil as expected by ...

Biochemical And Kinetic Analysis Of Arabidopsis Nucleobase Transport, Yvonne W. Gicheru

Master's Theses

Due to its relevance in plant growth, reproduction and development, inter-and intracellular transport of nucleobases remains to be an important area of basic research. In this work, two nucleobase transporters of Arabidopsis thaliana from two distinct families were partially characterized with the goal of determining their solute transport specificity. The first transporter studied is nucleobase ascorbate transporter 2 (AtNAT2), one of a 12-member putative gene family predicted to function as a nucleobase-ascorbate transporters. The coding sequence of AtNAT2 was cloned into a yeast expression vector driven by a yeast constitutive promoter. Yeast expressing AtNAT2 were tested through growth studies ...