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Chromosome Counts For Packera Paupercula Variety Gypsophila, Chad Larson 2014 Minnesota State University, Mankato

Chromosome Counts For Packera Paupercula Variety Gypsophila, Chad Larson

Journal of Undergraduate Research at Minnesota State University, Mankato

New plant species develop when diploid plants (having two sets of chromosomes) spontaneously double their chromosomes and the resulting tetraploids (having four sets of chromosomes) are no longer able to back cross to diploid members of the population. The North American plant species Packera paupercula (balsam-leafed ragwort), is widespread and morphologically diverse. An isolated group of populations from the chalky soils of west-central Alabama was recently named Packera paupercula variety gypsophila (the chalk-loving balsam ragwort). The variety’s large diameter pollen grains suggest it may be tetraploid. This project attempts the first chromosome count for the variety by examining cells ...


Characterization Of Fall Leaves As A Source Of Cellulosic Ethanol, Justin Burum 2014 Minnesota State University, Mankato

Characterization Of Fall Leaves As A Source Of Cellulosic Ethanol, Justin Burum

Journal of Undergraduate Research at Minnesota State University, Mankato

Ethanol is becoming increasingly popular as a fossil fuel additive or substitute. However, current production of ethanol from corn impacts food prices and appears to have an overall low net yield. New sources need to be identified and new processes developed for ethanol production. Cellulosic ethanol is one such new source. Plant material contains a large amount of cellulose and other polysaccharides which are potential feed stocks for ethanol production. The purpose of this experiment was to characterize the polysaccharide, lignin, and ash content of fall leaves to estimate their potential for ethanol production. A slight modification of the NREL ...


Expression Of 9/13 Hydroperoxide Lyase In Cucumber Leaves, Samee Ranginwala 2014 Minnesota State University, Mankato

Expression Of 9/13 Hydroperoxide Lyase In Cucumber Leaves, Samee Ranginwala

Journal of Undergraduate Research at Minnesota State University, Mankato

Fatty Acid 9/13-Hydroperoxide Lyase (9/13-HPL) in the cucumber plant is an enzyme that cleaves either 9- or 13-hydroperoxides of polyunsaturated fatty acids to form volatile C9 or C6 aldehydes respectively. Since these aldehydes may play a role in the plant’s defenses against pathogens (K. Matsui, et. al. Phytochemistry 67 (2006) 649-657), the enzyme is stress induced during vulnerable times such as when the plant is injured. In order to better understand how this enzyme is induced, we have developed a Real Time Polymerase Chain Reaction method to quantitate levels of 9/13-HPL mRNA. In initial experiments, the ...


Effects Of Culture Media And Plant Growth Regulators On Micropropagation Of Willow (Salix Matsudana ‘Golden Spiral’) And Hazelnut (Corylus Colurna ‘Te Terra Red), Dongxue Shi 2014 University of Nebraska - Lincoln

Effects Of Culture Media And Plant Growth Regulators On Micropropagation Of Willow (Salix Matsudana ‘Golden Spiral’) And Hazelnut (Corylus Colurna ‘Te Terra Red), Dongxue Shi

Theses, Dissertations, and Student Research in Agronomy and Horticulture

The branches and leaves of Salix matsudana ‘Golden Spiral’ , willow, grow in a twisted manner, which makes it an important bonsai plant. Its mature stems have potential for the woody cut floral industry. Corylus colurna ‘ Te Terra Red’, hazelnut, distinguished by red or purple leaves in the spring, has potential ornamental value in the horticulture industry for landscape use. Micropropagation of these two plants could provide more and healthier plantlets for rapid commercial scale-up by the nursery industry. Nas and Read Medium (NRM) is a newer medium developed specifically for hazelnut species by Nas and Read based on the composition ...


An Evaluation Of Watermelon (Citrullus Spp.) Germplasm For Additional Sources Of Resistance To The Twospotted Spider Mite (Tetranychus Urticae Koch)., Hector Cantu Jr. 2014 University of Nebraska - Lincoln

An Evaluation Of Watermelon (Citrullus Spp.) Germplasm For Additional Sources Of Resistance To The Twospotted Spider Mite (Tetranychus Urticae Koch)., Hector Cantu Jr.

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Fourteen U.S plant introduction (PI) accessions of C. lanatus var. lanatus (4), C. lanatus var. citroides (5) C. colocynthis (5) and a known susceptible commercial cultivar ‘Sugar Baby’ were evaluated for resistance to the twospotted spider mite, Tetranychus urticae Koch, in a limited free-choice and free-choice bioassay under laboratory conditions. The limited free choice bioassay, involved nine Petri dish cages that held five randomly assigned leaves individually inoculated with two adult females and one adult male. Eggs, larva, and adults were counted over a nine day period. The free choice bioassay involved the even distribution of three mite infested ...


Functional Genomics Of Maize Endosperm Maturation And Protein Quality, Lingling Yuan 2014 University of Nebraska - Lincoln

Functional Genomics Of Maize Endosperm Maturation And Protein Quality, Lingling Yuan

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Maize is one of the most important cereal crops and widely cultivated throughout the world. The study on maize kernel development including protein quality improvement is essential for removing dietary protein deficiency because of the lack of essential amino acids, especially lysine and tryptophan, in maize kernel. Quality Protein Maize (QPM) is a hard kernel variant of the high-lysine mutant, opaque-2. We created opaque QPM variants to identify opaque-2 modifier genes and to investigate deletion mutagenesis combined with Illumina sequencing as a maize functional genomics tool. A K0326Y-QPM deletion mutant, line 107, was null for the 27- and 50-kD γ-zeins ...


Improving Cocoa Production Through Lean: A Case Study Of The Ghanaian Cocoa Industry, Kwasi Boateng 2014 Western Kentucky University

Improving Cocoa Production Through Lean: A Case Study Of The Ghanaian Cocoa Industry, Kwasi Boateng

Masters Theses & Specialist Projects

Cocoa has been useful in several ways, especially to countries that produce it and consumers all over the world. Its benefits range from improving economies to satisfying millions all over the world who desire one cocoa product or the other. The production of cocoa however is not without its own attendant problems. Aging tree stock, spread of diseases, and production capacity problems are, but a few of many challenges that seed gardens that produce cocoa face. This study investigated the pollination process on selected seed gardens in Ghana, the second largest world producer of cocoa. The purpose of this study ...


Genetic Variation And Trait Associations For Forage Yield And Quality Among F1 Half-Sib Families Of Switchgrass, Matthew Eric Bobbitt 2014 University of Tennessee, Knoxville

Genetic Variation And Trait Associations For Forage Yield And Quality Among F1 Half-Sib Families Of Switchgrass, Matthew Eric Bobbitt

Masters Theses

Switchgrass (Panicum virgatum L.) is widely accepted as a forage crop throughout the United States. It is known for its performance on field sites that may be marginal for row crop production and as a warm season grass to fill gaps for cool season forages. With the increase of fuel and fertilizer costs, forage producers need higher yields and better quality than ever before. The objectives of this research were to: (i) compare four F1 [first generation] half-sib populations for their potential of producing superior lines for forage production, (ii) assess the genetic variances for yield, and (iii) evaluate ...


Genetic Variability For Biomass Yield, Yield Components, And Ethanol Yield Among Half-Sibs Of Switchgrass, Eifion Wyn Hughes 2014 University of Tennessee, Knoxville

Genetic Variability For Biomass Yield, Yield Components, And Ethanol Yield Among Half-Sibs Of Switchgrass, Eifion Wyn Hughes

Masters Theses

For bioenergy crops to be an economical alternative to fossil fuels, rapid biological and technological advancements will need to occur. Some advancements can be accomplished by producing new switchgrass (Panicum virgatum L.) varieties with higher biomass and ethanol yields. The objective of this research was to quantify biomass and ethanol yield potential of four F1 [F1] half-sib populations for future variety development as a bioenergy crop.

The four parental lines were PI 421999 (AR), PI 607837 (TX), PI 421552 (Cimarron), and Exp. # NSL-2001-1 (OK). Seed for one hundred and forty F1 [F1] half-sib progeny were produced in a ...


The Development Of Polyamines Throughout Brassica Rapa Over Its Lifecycle, Matthew Ryan Sullivan, Jon Larsen, Subhash C. Minocha 2014 University of New Hampshire

The Development Of Polyamines Throughout Brassica Rapa Over Its Lifecycle, Matthew Ryan Sullivan, Jon Larsen, Subhash C. Minocha

Student Research Projects

Polyamines are naturally produced chemicals in plants involved in growth, development and stress response. The primary objective of my study is to create a profile of changes in the entire life of the plant, in every organ at all stages of development from seed germination to seed formation. We have analyzed polyamines putrescine, spermidine and spermine in all parts of Brassica rapa, a small, rapid growing plant. Parallel to the polyamines, we will also study changes in the activities of the polyamine biosynthetic enzymes and the expression of their genes in different organs at different times. In the next stage ...


Eliminating Host-Mediated Effects Demonstrates Bt Maize Producing Cry1f Has No Adverse Effects On The Parasitoid Cotesia Marginiventris, Jun-Ce Tian, Xiang-Ping Wang, Li-Ping Long, Jörg Romeis, Steven E. Naranjo, Richard L. Hellmich, Anthony M. Shelton 2014 Iowa State University

Eliminating Host-Mediated Effects Demonstrates Bt Maize Producing Cry1f Has No Adverse Effects On The Parasitoid Cotesia Marginiventris, Jun-Ce Tian, Xiang-Ping Wang, Li-Ping Long, Jörg Romeis, Steven E. Naranjo, Richard L. Hellmich, Anthony M. Shelton

Entomology Publications and Papers

The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is an important pest of maize in the United States and many tropical areas in the western hemisphere. In 2001, Herculex I® (Cry1F) maize was commercially planted in the United States to control Lepidoptera, including S. frugiperda. In 2006, a population of S. frugiperda was discovered in Puerto Rico that had evolved resistance to Cry1F maize in the field, making it the first well-documented case of an insect with field resistance to a plant producing protein from Bacillus thuringiensis (Bt). Using this resistant population, we conducted tri-trophic studies with a ...


Reassessment Of Practical Subspecies Identifications Of The Usda Daucus Carota L. Germplasm Collection: Morphological Data, David M. Spooner, Mark P. Widrlechner, Kathleen R. Reitsma, Debra E. Palmquist, Slim Rouz, Zeineb Ghrabi-Gammar, Mohamed Neffati, Béchir Bouzbida, Philipp W. Simon, Mohammed El Koudrim 2014 Iowa State University

Reassessment Of Practical Subspecies Identifications Of The Usda Daucus Carota L. Germplasm Collection: Morphological Data, David M. Spooner, Mark P. Widrlechner, Kathleen R. Reitsma, Debra E. Palmquist, Slim Rouz, Zeineb Ghrabi-Gammar, Mohamed Neffati, Béchir Bouzbida, Philipp W. Simon, Mohammed El Koudrim

NCRPIS Publications and Papers

The genus Daucus includes about 20 recognized species. The most widespread and economically important species, Daucus carota L., occurs on almost every continent. The cultivated carrot, subsp. sativus (Hoffm.) Schübl. and G. Martens, has been selected from wild populations that are extremely diverse, especially in the western Mediterranean. The predominant outcrossing and the lack of sexual isolating mechanisms among recognized infraspecific taxa complicate the taxonomy and identification of the wild populations, resulting in widely different interpretations of the number of infraspecific taxa. We measured 36 morphological characters from multiple individuals within each of 155 accessions of D. carota and from ...


Registration Of ‘Mattern’ Waxy (Amylose-Free) Winter Wheat, P S. Baenziger, R A. Graybosch, D K. Santra, T Regassa, Y Jin, S Wegulo, G Bai, P S. Amand, X Chen, B Seabourn, F Dowell, R Bowden, D M. Marshall 2014 University of Nebraska, Lincoln

Registration Of ‘Mattern’ Waxy (Amylose-Free) Winter Wheat, P S. Baenziger, R A. Graybosch, D K. Santra, T Regassa, Y Jin, S Wegulo, G Bai, P S. Amand, X Chen, B Seabourn, F Dowell, R Bowden, D M. Marshall

Teshome H. Regassa

‘Mattern’ (Reg. No. CV-1092, PI 665947) hard winter waxy wheat (Triticum aestivum L.) was developed cooperatively by the USDA–ARS and the Nebraska Agricultural Experiment Station and released in 2012. Mattern has red grain color and waxy (amylose-free) endosperm starch. It was released primarily for its unique end-use quality attributes and for grain yield competitive with currently grown Nebraskaadapted cultivars. The waxy starch is conditioned by the presence of three naturally occurring mutations that eliminate production of the enzyme granule-bound starch synthase. Granule-bound starch synthase synthesizes amylose in typical wheats and other cereal crops. Mattern was tested experimentally as NX04Y2107 ...


Using Resistant Prey Demonstrates That Bt Plants Producing Cry1ac, Cry2ab, And Cry1f Have No Negative Effects On Geocoris Punctipes And Orius Insidiosus, Jun-Ce Tian, Li-Ping Long, Xiang-Ping Wang, Steven E. Naranjo, Jörg Romeis, Richard L. Hellmich, Ping Wang, Anthony M. Shelton 2014 Iowa State University

Using Resistant Prey Demonstrates That Bt Plants Producing Cry1ac, Cry2ab, And Cry1f Have No Negative Effects On Geocoris Punctipes And Orius Insidiosus, Jun-Ce Tian, Li-Ping Long, Xiang-Ping Wang, Steven E. Naranjo, Jörg Romeis, Richard L. Hellmich, Ping Wang, Anthony M. Shelton

Entomology Publications and Papers

Geocoris punctipes (Say) and Orius insidiosus (Say) are generalist predators found in a wide range of crops, including cotton (Gossypium hirsutum L. ) and maize (Zea mays L. ), where they provide important biological control services by feeding on an array of pests, including eggs and small larvae of caterpillars. A high percentage of cotton and maize in the United States and several other countries are transgenic cultivars that produce one or more of the insecticidal Cry proteins ofBacillus thuringiensis Berliner (Bt). Here we quantify effects of three Cry proteins on the life history of these predators over two generations when ...


Fusarium Head Blight Resistance And Agronomic Performance In Soft Red Winter Wheat Populations, Daniela Sarti Dvorjak 2014 University of Kentucky

Fusarium Head Blight Resistance And Agronomic Performance In Soft Red Winter Wheat Populations, Daniela Sarti Dvorjak

Theses and Dissertations--Plant and Soil Sciences

Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe [telomorph: Gibberella zeae Schwein.(Petch)], is recognized as one of the most destructive diseases of wheat (Triticum aestivum L. and T. durum L.) and barley (Hordeum vulgare L.) worldwide. Breeding for FHB resistance must be accompanied by selection for desirable agronomic traits. Donor parents with two FHB resistance quantitative trait loci (QTL) Fhb1 (chromosome 3BS) and QFhs.nau-2DL (chromosome 2DL) were crossed to four adapted SRW wheat lines to generate backcross and forward cross progeny. F2 individuals were genotyped and assigned to 4 different groups according to presence/ absence of ...


Testing The Fertility And Allelopathic Abilities Of Arctotheca Prostrata (Salisb.) Britten (Asteraceae, Arctotideae), A South African Plant Species That Has Naturalized In California, Jordy Veit 2014 Minnesota State University, Mankato

Testing The Fertility And Allelopathic Abilities Of Arctotheca Prostrata (Salisb.) Britten (Asteraceae, Arctotideae), A South African Plant Species That Has Naturalized In California, Jordy Veit

All Theses, Dissertations, and Other Capstone Projects

Arctotheca prostrata is a perennial plant species native to South Africa that reproduces vegetatively by long runners. In South Africa it also reproduces sexually, producing fruits with fertile seeds. The species was brought to California to serve as a ground cover but fruits had not been reported. Historically, it has been said to be infertile. This study tested the fertility of A. prostrata in California by crossing plants from California with each other and with plants grown from imported South African seeds. Pollen viability was tested and morphological measures were made on heads, leaves, and pollen. Mean Californian and South ...


The Performance Of Bacterial Phytosensing Transgenic Tobacco Under Field Conditions, Michael Harrison Fethe 2013 University of Tennessee, Knoxville

The Performance Of Bacterial Phytosensing Transgenic Tobacco Under Field Conditions, Michael Harrison Fethe

Masters Theses

Currently the platforms for wide-area detection of environmental contamination are limited. Therefore, there is interest in developing new platforms, especially for use in crop plants to detect and report the presence of biotic and abiotic stress agents. A biosensor uses a biological organism or substrate to detect the presence of an elicitor (i.e., heavy metal, TNT, or bacteria). The foundational groundwork to create biosensors in transgenic plants exists. The creation of bacterial phytosensing transgenic tobacco containing an orange fluorescent protein (OFP) reporter driven by synthetic pathogen-inducible promoters provides a fluorescent signal when infected with phytopathogens for earlier detection in ...


Availability And Accessibility Of Research Outputs In Nars: A Case Study With Iari, Gitanjali Tandon, Sridhar Gutam, Lalitha Poluru, A K. Mishra, P S. Pandey, Chandrasekharan H 2013 SelectedWorks

Availability And Accessibility Of Research Outputs In Nars: A Case Study With Iari, Gitanjali Tandon, Sridhar Gutam, Lalitha Poluru, A K. Mishra, P S. Pandey, Chandrasekharan H

Sridhar Gutam

This article focuses on the trends in publication, authorship pattern, availability, and accessibility of articles during 2008–10 from the Indian Agricultural Research Institute (IARI), a constituent of the National Agricultural Research System in India. The data reveal that during the period of study, researchers from IARI produced 1,833 publications, most of which were jointly authored, and that the most preferred journal for publication by researchers is the Indian Journal of Agricultural Science, which is now an Open Access journal. While publications from IARI are available to subscribers of the Consortium for e-Resources in Agriculture (CeRA), public availability to ...


Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati 2013 SelectedWorks

Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati

Rajesh Pati

Mango (Mangifera indica L.) is the most important fruit crop of the world. India ranks first among world’s mango producing countries accounting for 57.18 % of the total world mango production of 19.22 million tones. With the development of rDNA technology, plant breeders have access to a large number of genes that can be integrated into the plant genome.


Economic Impact Assessment Of Sorghum, Millet And Other Grains Crsp: Sorghum And Millet Germplasm Development Research, Timothy J. Dalton, Yacob A. Zereyesus 2013 University of Nebraska - Lincoln

Economic Impact Assessment Of Sorghum, Millet And Other Grains Crsp: Sorghum And Millet Germplasm Development Research, Timothy J. Dalton, Yacob A. Zereyesus

INTSORMIL Scientific Publications

As a result of the offsetting effect of a rise in productivity and decline in harvested area, the world sorghum production (tonnes) has been on a horizontal trend. The combined effect of a decline in area harvested and a rise in yield productivity have resulted in an overall increase in world millet production (tonnes) trend.

During the last three decades, significant numbers of breeding lines, parental stocks, germplasm and cultivars have been released through INTSORMIL/host countries collaboration around the world. Some of the remarkable breeding success stories include the release of the first hybrid sorghum Hageen Dura (HD-1) and ...


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