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Stability Analysis Of Kernel Quality Traits In Exotic-Derived Doubled Haploid Maize Lines, Adam Vanous, Candice Gardner, Michael Blanco, Adam Martin-Schwarze, Jinyu Wang, Xianran Li, Alexander E. Lipka, Sherry Flint-Garcia, Martin Bohn, Jode Edwards, Thomas Lubberstedt 2019 Iowa State University

Stability Analysis Of Kernel Quality Traits In Exotic-Derived Doubled Haploid Maize Lines, Adam Vanous, Candice Gardner, Michael Blanco, Adam Martin-Schwarze, Jinyu Wang, Xianran Li, Alexander E. Lipka, Sherry Flint-Garcia, Martin Bohn, Jode Edwards, Thomas Lubberstedt

Agronomy Publications

Variation in kernel composition across maize (Zea mays L.) germplasm is affected by a combination of the plant’s genotype, the environment in which it is grown, and the interaction between these two elements. Adapting exotic germplasm to the US Corn Belt is highly dependent on the plant’s genotype, the environment where it is grown, and the interaction between these components. Phenotypic plasticity is ill-defined when specific exotic germplasm is moved over large latitudinal distances and for the adapted variants being created. Reduced plasticity (or stability) is desired for the adapted variants, as it allows for a more rapid ...


Risks And Opportunities Of Gm Crops: Bt Maize Example, Andrea K. Carzoli, Siddique I. Aboobucker, Leah L. Sandall, Thomas T. Lubberstedt, Walter P. Suza 2018 Iowa State University

Risks And Opportunities Of Gm Crops: Bt Maize Example, Andrea K. Carzoli, Siddique I. Aboobucker, Leah L. Sandall, Thomas T. Lubberstedt, Walter P. Suza

Agronomy Publications

The anticipated world population growth emphasizes a need to produce more food on less land. Cutting-edge technologies, including genetic engineering, can help to develop improved crop varieties and protect natural resources. In spite of the potential for genetically-modified (GM) crops to make crop production more efficient, they remain a polarizing issue due to safety concerns. This paper provides an overview of the risk assessment process. The safety of Bacillus thuringiensis (Bt) proteins is used as an example for how risk assessment is applied to GM crops. Risks associated with GM crops have proven to be low to non-existent. Developing countries ...


Influence Of Loss Of Function Of The Fatty Acid Desaturase 7 Gene On Photosynthetic Activity And Foliar Redox Status, Janithri Savindya Wickramanayake 2018 University of Arkansas, Fayetteville

Influence Of Loss Of Function Of The Fatty Acid Desaturase 7 Gene On Photosynthetic Activity And Foliar Redox Status, Janithri Savindya Wickramanayake

Theses and Dissertations

Fatty Acid Desaturase7 (FAD7) is a chloroplast-localized enzyme that converts 16 and 18 carbon dienoic fatty acids to trienoic fatty acids. The suppressor of prosystemin-mediated response2 (spr2) mutant in tomato (Solanum lycopersicum) and the fad7-1 mutant in Arabidopsis (Arabidopsis thaliana) result in the loss of function of FAD7, which alter the fatty acid profiles of chloroplast membranes and enhance resistance against aphids. This research contributes toward the long-term goal of identifying factors that determine aphid resistance in FAD7 mutants. Previous data suggested constitutive differences between spr2 and WT including increased expression of genes associated with photosynthesis and differences in redox ...


Linkage Mapping For Soybean (Glycine Max) Flood Tolerance, Wade Stiles Hummer 2018 University of Arkansas, Fayetteville

Linkage Mapping For Soybean (Glycine Max) Flood Tolerance, Wade Stiles Hummer

Theses and Dissertations

Flood tolerance in soybean (Glycine max) is not a well-characterized trait, yet flooding damage is second only to drought stress in terms of yield reduction. The objectives of this study were to determine genetic variation for flooding tolerance in two populations of soybean recombinant inbred lines (RILs) and to identify and confirm flood tolerant QTL. Population A (WHA) consisted of 111 RILs derived from the cross 5002T by 91210-350 and Population B (WHB) consisted of 79 RILs from the cross RA-452 by Osage. Experiments were conducted at the Rice Research and Extension Center (RREC) near Stuttgart, AR in 2015 and ...


Generation And Evaluation Of Modified Opaque-2 (O2) Popcorn Suggests A Route To Quality Protein Popcorn (Qpp), Ying Ren 2018 University of Nebraska-Lincoln

Generation And Evaluation Of Modified Opaque-2 (O2) Popcorn Suggests A Route To Quality Protein Popcorn (Qpp), Ying Ren

Theses, Dissertations, and Student Research in Agronomy and Horticulture

I have been working on a Quality Protein Popcorn breeding project where QPM conversion is carried out simultaneously for several elite popcorn germplasms. During my study in the graduate program, I led the following aspects of the Quality Protein Popcorn Breeding Project:

1. Identified suitable QPMs as opaque-2 allele donors.

2.Examined the feasibility of quick introgression of the opaque-2 allele into popcorn lines via marker-assisted selection.

3. Monitored modification by SDS-PAGE zein profiling and light box phenotypic selection to make sure multiple modifier loci for opaque-2 were incorporated each time generation advancement was carried out.

4. Carried out high ...


Insights Into The Evolution Of The New World Diploid Cottons (Gossypium, Subgenus Houzingenia) Based On Genome Sequencing, Corrinne E. Grover, Mark A. Arick II, Adam Thrash, Justin L. Conover, William S. Sanders, Daniel G. Peterson, James E. Frelichowski, Jodi A. Scheffler, Brian E. Scheffler, Jonathan F. Wendel 2018 Iowa State University

Insights Into The Evolution Of The New World Diploid Cottons (Gossypium, Subgenus Houzingenia) Based On Genome Sequencing, Corrinne E. Grover, Mark A. Arick Ii, Adam Thrash, Justin L. Conover, William S. Sanders, Daniel G. Peterson, James E. Frelichowski, Jodi A. Scheffler, Brian E. Scheffler, Jonathan F. Wendel

Ecology, Evolution and Organismal Biology Publications

We employed phylogenomic methods to study molecular evolutionary processes and phylogeny in the geographically widely dispersed New World diploid cottons (Gossypium, subg. Houzingenia). Whole genome resequencing data (average of 33X genomic coverage) were generated to reassess the phylogenetic history of the subgenus and provide a temporal framework for its diversification. Phylogenetic analyses indicate that the subgenus likely originated following trans-oceanic dispersal from Africa about 6.6 mya, but that nearly all of the biodiversity evolved following rapid diversification in the mid-Pleistocene (0.5-2.0 mya), with multiple long-distance dispersals required to account for range expansion to Arizona, the Galapagos Islands ...


Haplotype Loci Under Selection In Canadian Durum Wheat Germplasm Over 60 Years Of Breeding: Association With Grain Yield, Quality Traits, Protein Loss, And Plant Height, Amidou N'Diaye, Jemanesh K. Haile, Kirby T. Nilsen, Sean Walkowiak, Yuefeng Ruan, Asheesh K. Singh, Fran R. Clarke, John M. Clarke, Curtis J. Pozniak 2018 University of Saskatchewan

Haplotype Loci Under Selection In Canadian Durum Wheat Germplasm Over 60 Years Of Breeding: Association With Grain Yield, Quality Traits, Protein Loss, And Plant Height, Amidou N'Diaye, Jemanesh K. Haile, Kirby T. Nilsen, Sean Walkowiak, Yuefeng Ruan, Asheesh K. Singh, Fran R. Clarke, John M. Clarke, Curtis J. Pozniak

Agronomy Publications

Durum wheat was introduced in the southern prairies of western Canada in the late nineteenth century. Breeding efforts have mainly focused on improving quality traits to meet the pasta industry demands. For this study, 192 durum wheat lines were genotyped using the Illumina 90K Infinium iSelect assay, and resulted in a total of 14,324 polymorphic SNPs. Genetic diversity changed over time, declining during the first 20 years of breeding in Canada, then increased in the late 1980s and early 1990s. We scanned the genome for signatures of selection, using the total variance Fst-based outlier detection method (Lositan), the hierarchical ...


Genotypic Variation In Anthocyanins, Phenolic Compounds, And Antioxidant Activity In Cob And Husk Of Purple Field Corn, Ponsawan Khamphasan, Khomsorn Lomthaisong, Bhornchai Harakotr, Danupol Ketthaisong, Marvin Paul Scott, Kamol Lertrat, Bhalang Suriharn 2018 Khon Kaen University

Genotypic Variation In Anthocyanins, Phenolic Compounds, And Antioxidant Activity In Cob And Husk Of Purple Field Corn, Ponsawan Khamphasan, Khomsorn Lomthaisong, Bhornchai Harakotr, Danupol Ketthaisong, Marvin Paul Scott, Kamol Lertrat, Bhalang Suriharn

Agronomy Publications

Information on phytochemicals in the cob and husk of field corn is important for the use of corn waste in the production of value-added corn products. The objectives of this study were to evaluate the variation in monomeric anthocyanin content (MAC), total phenolic content (TPC), and antioxidant activity, as determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) in 53 purple field corn genotypes, and to study the correlations of these traits with color parameters. Fifty-three corn genotypes were planted in a randomized complete block design with three replications in two locations in the ...


High Density Genetic Mapping Of Fusarium Head Blight Resistance Qtl In Tetraploid Wheat, Ehsan Sari, Samia Berraies, Ron E. Knox, Asheesh K. Singh, Yuefeng Ruan, Richard D. Cuthbert, Curtis J. Pozniak, Maria Antonia Henriquez, Santosh Kumar, Andrew J. Burt, Amidou N'Diaye, David J. Konkin, Adrian L. Cabral, Heather L. Campbell, Krystalee Wiebe, Janet Condie, Prabhath Lokuruge, Brad Meyer, George Fedak, John M. Clarke, Daryl J. Somers, Perre R. Fobert 2018 Agriculture and Agri-Food Canada

High Density Genetic Mapping Of Fusarium Head Blight Resistance Qtl In Tetraploid Wheat, Ehsan Sari, Samia Berraies, Ron E. Knox, Asheesh K. Singh, Yuefeng Ruan, Richard D. Cuthbert, Curtis J. Pozniak, Maria Antonia Henriquez, Santosh Kumar, Andrew J. Burt, Amidou N'Diaye, David J. Konkin, Adrian L. Cabral, Heather L. Campbell, Krystalee Wiebe, Janet Condie, Prabhath Lokuruge, Brad Meyer, George Fedak, John M. Clarke, Daryl J. Somers, Perre R. Fobert

Agronomy Publications

Breeding for Fusarium head blight (FHB) resistance in durum wheat is complicated by the quantitative trait expression and narrow genetic diversity of available resources. High-density mapping of the FHB resistance quantitative trait loci (QTL), evaluation of their co-localization with plant height and maturity QTL and the interaction among the identified QTL are the objectives of this study. Two doubled haploid (DH) populations, one developed from crosses between Triticum turgidum ssp. durum lines DT707 and DT696 and the other between T. turgidum ssp. durum cv. Strongfield and T. turgidum ssp. carthlicum cv. Blackbird were genotyped using the 90K Infinium iSelect chip ...


Color Space Standardization And Image Analysis For High-Throughput Phenotyping Of Sorghum Bicolor, Alexandria A. Pokorny 2018 Department of Mathematics, Illinois State University

Color Space Standardization And Image Analysis For High-Throughput Phenotyping Of Sorghum Bicolor, Alexandria A. Pokorny

Annual Symposium on Biomathematics and Ecology: Education and Research

No abstract provided.


Aac Viewfield Hard Red Spring Wheat, R. D. Cuthbert, R. M. DePauw, A. K. Singh, B. McCallum, T. Fetch 2018 Agriculture and Agri-Food Canada

Aac Viewfield Hard Red Spring Wheat, R. D. Cuthbert, R. M. Depauw, A. K. Singh, B. Mccallum, T. Fetch

Agronomy Publications

AAC Viewfield hard red spring wheat (Triticum aestivum L.) has grain yield significantly higher than the check cultivars Katepwa and Lillian and is similar to Carberry. AAC Viewfield matures significantly later than Katewpa and Lillian but is similar to Carberry. AAC Viewfield has an awned spike, a low lodging score indicative of strong straw, and significantly shorter plant stature than all checks. AAC Viewfield expressed resistance to prevalent races of yellow rust and stem rust, moderate resistance to leaf rust and common bunt, and intermediate resistance to Fusarium head blight. AAC Viewfield has quality attributes within the range of the ...


Multi-Objective Optimized Genomic Breeding Strategies For Sustainable Food Improvement, Deniz Akdemir, William D, Beavis, Roberto Fritsche-Neto, Asheesh K. Singh, Julio Isidro-Sánchez 2018 Cornell University

Multi-Objective Optimized Genomic Breeding Strategies For Sustainable Food Improvement, Deniz Akdemir, William D, Beavis, Roberto Fritsche-Neto, Asheesh K. Singh, Julio Isidro-Sánchez

Agronomy Publications

The purpose of breeding programs is to obtain sustainable gains in multiple traits while controlling the loss of genetic variation. The decisions at each breeding cycle involve multiple, usually competing, objectives; these complex decisions can be supported by the insights that are gained by applying multi-objective optimization principles to breeding. The discussion in this manuscript includes the definition of several multi-objective optimized breeding approaches within the phenotypic or genomic breeding frameworks and the comparison of these approaches with the standard multi-trait breeding schemes such as tandem selection, independent culling and index selection. Proposed methods are demonstrated with two empirical data ...


Identification Of Quantitative Trait Loci (Qtl) For Tolerance To Metribuzin In Soft Red Winter Wheat (Triticum Aestivum L.), Hugo Alfonso Anzueto Ponciano 2018 Louisiana State University and Agricultural and Mechanical College

Identification Of Quantitative Trait Loci (Qtl) For Tolerance To Metribuzin In Soft Red Winter Wheat (Triticum Aestivum L.), Hugo Alfonso Anzueto Ponciano

LSU Master's Theses

Wheat is one of the world’s most important food crops and provides nearly 20% of the calories consumed. Wheat is the third most important crop after corn and rice on a global production scale. In Louisiana wheat harvest was about 6000 hectares in 2017. Wheat production is negatively affected by several biotic and abiotic factors including weather, diseases, and weeds. Metribuzin is a broad-spectrum herbicide commonly used in wheat because it is inexpensive and controls most of the common weeds in wheat fields. Metribuzin has benefits for the grower controlling weeds that might cause yield loss in varieties or ...


Yardwork: A Biography Of An Urban Place By Daniel Coleman, Vivian M. Hansen 2018 University of Calgary

Yardwork: A Biography Of An Urban Place By Daniel Coleman, Vivian M. Hansen

The Goose

Review of Daniel Coleman's Yardwork: A Biography of an Urban Place.


Genetic Diversity In An Invasive Clonal Plant? A Historical And Contemporary Perspective, Elliot D. Weidow 2018 University of New Orleans

Genetic Diversity In An Invasive Clonal Plant? A Historical And Contemporary Perspective, Elliot D. Weidow

University of New Orleans Theses and Dissertations

Introduced populations of Eichhornia crassipes (Pontederiaceae) possess extremely low levels of genetic diversity due to severe bottleneck events and clonal reproduction. While populations elsewhere have been well studied, North American populations of E. crassipes remain understudied. We used Amplified Fragment Length Polymorphism markers to assess genetic diversity and population structure in North American E. crassipes populations. Patterns of diversity over the past fifty years were analyzed using herbarium specimens. Furthermore, we sampled populations across the Gulf Coast of the United States throughout a year to determine contemporary genetic diversity and assess potential seasonal effects. Genetic diversity was found to be ...


Genetic Control And Geo-Climate Adaptation Of Pod Dehiscence Provide Novel Insights Into The Soybean Domestication And Expansion, Jiaoping Zhang, Asheesh K. Singh 2018 Iowa State University

Genetic Control And Geo-Climate Adaptation Of Pod Dehiscence Provide Novel Insights Into The Soybean Domestication And Expansion, Jiaoping Zhang, Asheesh K. Singh

Agronomy Publications

Loss of pod dehiscence is a key step during soybean [Glycine max (L.) Merr.] domestication. Genome-wide association analysis for soybean shattering identified loci harboring Pdh1, NST1A and SHAT1-5. Pairwise epistatic interactions were observed, and the dehiscent Pdh1 overcomes the resistance conferred by NST1A or SHAT1-5 locus, indicating that Pdh1 predominates pod dehiscence expression. Further candidate gene association analysis identified a nonsense mutation in NST1A associated with pod dehiscence. Allele composition and population differential analyses unraveled that Pdh1 and NST1A, but not SHAT1-5, underwent domestication and modern breeding selections. Geographic analysis showed that in Northeast China (NEC), indehiscence at both Pdh1 ...


Expression Of Carbohydrates Biosynthetic Genes In Developing Soybean Seeds, Jayden Rosen, Karen A. Hudson 2018 Purdue

Expression Of Carbohydrates Biosynthetic Genes In Developing Soybean Seeds, Jayden Rosen, Karen A. Hudson

The Summer Undergraduate Research Fellowship (SURF) Symposium

An essential part of livestock diets is soybean meal, which is a major source of protein, but which also consists of antinutritional carbohydrates. Antinutritional carbohydrates such as raffinose and stachyose lead to irritation to the gut for monogastric livestock as well as unhealthy weight gain. A major objective of soybean genetics is to reduce these antinutritional carbohydrates within the seed and increase the levels of good carbohydrates. This will lead to healthier livestock and better meat quality. To select genes potentially responsible for variation in carbohydrate levels in seeds, the expression of genes encoding several biosynthetic enzymes was measured during ...


Identification And Characterization Of Salinity Tolerance Genes By Activation Tagging In Arabidopsis, Jawaher Alkahtani 2018 University of Arkansas, Fayetteville

Identification And Characterization Of Salinity Tolerance Genes By Activation Tagging In Arabidopsis, Jawaher Alkahtani

Theses and Dissertations

Salinity often affects irrigated areas in arid and semi-arid regions of the world. The existence and accumulation of soluble salts in the soil layers limit the growth of crops essential for our food. Salt stress dramatically affects plant growth, plant development, as well as crop yield. Arabidopsis thaliana is the plant model that provides a comprehensive knowledge of plant development, genetics and physiology, and response to abiotic stresses such as salinity. The redundancy of genes due to duplication, even in the simple model genome of Arabidopsis, limits the value of knockout (KO) mutagenesis to provide complete information on gene function ...


Dissection Of Soil Waterlogging Tolerance In Soft Red Winter Wheat Using Genomic Approaches, Marlovi Andrea Acuna-Galindo 2018 University of Arkansas, Fayetteville

Dissection Of Soil Waterlogging Tolerance In Soft Red Winter Wheat Using Genomic Approaches, Marlovi Andrea Acuna-Galindo

Theses and Dissertations

Genomic methods including genome wide association analysis (GWAS), genomic selection (GS) and RNA-seq allow for faster selection of superior breeding lines and for identification and resolution of candidate genes. A panel of 240 soft red winter wheat (Triticum aestivum L.) cultivars and breeding lines were subjected to soil waterlogging stress over two seasons at Stuttgart, AR and St. Joseph, LA, US. Total concentrations of P, K, Ca, Mg, Mn, Fe, Al, B, Cu, Na, S and Zn were determined in wheat shoots post-waterlogging using inductively coupled plasma spectroscopy. Yield components kernel number per spike (KNPS), kernel weight per spike (KWS ...


Association Mapping And Genomic Selection For Yield And Agronomic Traits In Soft Winter Wheat, Dennis Bulusan Lozada 2018 University of Arkansas, Fayetteville

Association Mapping And Genomic Selection For Yield And Agronomic Traits In Soft Winter Wheat, Dennis Bulusan Lozada

Theses and Dissertations

Tools such as genome-wide association study (GWAS) and genomic selection (GS) have expedited the development of crops with improved genetic potential. While GWAS aims to identify significant markers associated with a trait of interest, the goal of GS is to utilize all marker effects to predict the performance of new breeding lines prior to testing. A GWAS for grain yield (GY), yield components, and agronomic traits was conducted using a diverse panel of 239 soft winter wheat (SWW) lines evaluated in eight site-years in Arkansas and Oklahoma. Broad sense heritability of GY (H2=0.48) was moderate compared to other ...


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