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Towards The Development Of A Sustainable Soya Bean-Based Feedstock For Aquaculture, Hyunwoo Park, Steven Weier, Fareha Razvi, Pamela A. Peña, Neil A. Sims, Jennica Lowell, Cory Hungate, Karma Kissinger, Gavin Key, Paul Fraser, Jonathan Napier, Edgar B. Cahoon, Thomas Clemente 2017 University of Nebraska-Lincoln

Towards The Development Of A Sustainable Soya Bean-Based Feedstock For Aquaculture, Hyunwoo Park, Steven Weier, Fareha Razvi, Pamela A. Peña, Neil A. Sims, Jennica Lowell, Cory Hungate, Karma Kissinger, Gavin Key, Paul Fraser, Jonathan Napier, Edgar B. Cahoon, Thomas Clemente

Faculty Publications from the Center for Plant Science Innovation

Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean-based protein and a high Omega-3 fatty acid soya bean oil, enriched with alpha-linolenic and ...


Supplemental Data, Russell J. Ingram, Foster Levy, Cindy L. Barrett, James T. Donaldson 2017 University of Georgia

Supplemental Data, Russell J. Ingram, Foster Levy, Cindy L. Barrett, James T. Donaldson

ETSU Faculty Works

No abstract provided.


Glucose-Abl1-Tor Signaling Modulates Cell Cycle Tuning To Control Terminal Appressorial Cell Differentiation, Margarita Marroquin-Guzman, Guangchao Sun, Richard Wilson 2017 University of Nebraska-Lincoln

Glucose-Abl1-Tor Signaling Modulates Cell Cycle Tuning To Control Terminal Appressorial Cell Differentiation, Margarita Marroquin-Guzman, Guangchao Sun, Richard Wilson

Papers in Plant Pathology

The conserved target of rapamycin (TOR) pathway integrates growth and development with available nutrients, but how cellular glucose controls TOR function and signaling is poorly understood. Here, we provide functional evidence from the devastating rice blast fungus Magnaporthe oryzae that glucose can mediate TOR activity via the product of a novel carbon- responsive gene, ABL1, in order to tune cell cycle progression during infection-related development. Under nutrient-free conditions, wild type (WT) M. oryzae strains form terminal plant-infecting cells (appressoria) at the tips of germ tubes emerging from three-celled spores (conidia). WT appressorial development is accompanied by one round of mitosis ...


Expression Of Apoplast-Targeted Plant Defensin Mtdef4.2 Confers Resistance To Leaf Rust Pathogen Puccinia Triticina But Does Not Affect Mycorrhizal Symbiosis In Transgenic Wheat, Jagdeep Kaur, John Fellers, Alok Adholeya, Siva L.S. Velivelli, Kaoutar El-Mounadi, Natalya Nersesian, Thomas E. Clemente, Dilip Shah 2017 Donald Danforth Plant Science Center

Expression Of Apoplast-Targeted Plant Defensin Mtdef4.2 Confers Resistance To Leaf Rust Pathogen Puccinia Triticina But Does Not Affect Mycorrhizal Symbiosis In Transgenic Wheat, Jagdeep Kaur, John Fellers, Alok Adholeya, Siva L.S. Velivelli, Kaoutar El-Mounadi, Natalya Nersesian, Thomas E. Clemente, Dilip Shah

Faculty Publications from the Center for Plant Science Innovation

Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control leaf rust, but genetic resistance is ephemeral and breaks down with the emergence of new virulent Pt races. There is a need to develop alternative measures for control of leaf rust in wheat. Development of transgenic wheat expressing an antifungal defensin offers a promising approach to complement the endogenous resistance genes within the wheat ...


Chemical And Non-Chemical Control Of Potato Pink Rot, Xuemei (Missi) Zhang 2016 The University of Maine

Chemical And Non-Chemical Control Of Potato Pink Rot, Xuemei (Missi) Zhang

Electronic Theses and Dissertations

Pink rot of potato (Solanum tuberosum) is a widespread soilborne disease that causes significant losses in the field and storage. It is caused by Phytophthora erythroseptica (Pethybr.), an oomycete pathogen that produces sexual spores that can survive in soil for years. The management of pink rot mainly relies on chemical control. However, the most effective chemical in pink rot control, mefenoxam, is losing its efficacy owing to the development of mefenoxam resistance in P. erythroseptica. To evaluate alternative fungicides (including chemical and biological fungicides) to mefenoxam in pink rot control, two greenhouse experiments and three field trials were conducted. Crop ...


Improved Fungicidal Control Of Large Patch Through Optimal Use Of Surfactants And Spray Rate Volume, Jesse J. Benelli 2016 University of Tennessee, Knoxville

Improved Fungicidal Control Of Large Patch Through Optimal Use Of Surfactants And Spray Rate Volume, Jesse J. Benelli

Doctoral Dissertations

Large patch (Rhizoctonia solani AG 2-2LP) epidemics cause significant damage to Japanese lawngrass (JLG; Zoysia japonica) in the transition zone. Large patch primarily affects the stems and sheaths of JLG and is difficult to control using traditional fungicide sprays. Field and growth chamber experiments were conducted during 2015-2016 in TN and GA to evaluate methods to enhance fungicidal control of large patch in JLG landscapes. The 1st experiment evaluated the most critical application target site that resulted in the greatest amount large patch control. In this experiment, four fungicides (azoxystrobin, flutolanil, tebuconazole, and chlorothalonil) were dispensed as single droplets ...


Predators Catalyze An Increase In Chloroviruses By Foraging On The Symbiotic Hosts Of Zoochlorellae, John DeLong, Zeina Al-Ameeli, Garry A. Duncan, James Van Etten, David D. Dunigan Ph. D. 2016 University of Nebraska-Lincoln

Predators Catalyze An Increase In Chloroviruses By Foraging On The Symbiotic Hosts Of Zoochlorellae, John Delong, Zeina Al-Ameeli, Garry A. Duncan, James Van Etten, David D. Dunigan Ph. D.

James Van Etten Publications

Virus population growth depends on contacts between viruses and their hosts. It is often unclear how sufficient contacts are made between viruses and their specific hosts to generate spikes in viral abundance. Here, we show that copepods, acting as predators, can bring aquatic viruses and their algal hosts into contact. Specifically, predation of the protist Paramecium bursaria by copepods resulted in a >100-fold increase in the number of chloroviruses in 1 d. Copepod predation can be seen as an ecological “catalyst” by increasing contacts between chloroviruses and their hosts, zoochlorellae (endosymbiotic algae that live within paramecia), thereby facilitating viral population ...


Molecular Characterization Of Viruses Infecting Greenhouse Vegetables In Ontario, Bin Chen 2016 The University of Western Ontario

Molecular Characterization Of Viruses Infecting Greenhouse Vegetables In Ontario, Bin Chen

Electronic Thesis and Dissertation Repository

Greenhouse vegetable production is a very important and successful agricultural sector in Ontario. However, newly emerging viral or virus-like diseases have become a major limiting factor in greenhouse vegetable production. To determine the identity of the viral pathogens in the three major greenhouse vegetables in Ontario, we conducted in-depth sequencing of small RNAs isolated from virally infected plants used next-generation sequencing technology. Subsequent bioinformatics analyses revealed six viral pathogens including Bell pepper endornavirus (BPEV), Parietaria mottle virus (PMoV), Cucumber green mottle mosaic virus (CGMMV), Tobacco streak virus (TSV), Pepper mild mottle virus (PMMoV), and Pepino mosaic virus (PepMV). The full-length ...


Orm Expression Alters Sphingolipid Homeostasis And Differentially Affects Ceramide Synthase Activity, Athen N. Kimberlin, Gongshe Han, Kyle D. Luttgeharm, Ming Chen, Rebecca E. Cahoon, Julie M. Stone, Jonathan E. Markham, Teresa M. Dunn, Edgar B. Cahoon 2016 University of Nebraska - Lincoln

Orm Expression Alters Sphingolipid Homeostasis And Differentially Affects Ceramide Synthase Activity, Athen N. Kimberlin, Gongshe Han, Kyle D. Luttgeharm, Ming Chen, Rebecca E. Cahoon, Julie M. Stone, Jonathan E. Markham, Teresa M. Dunn, Edgar B. Cahoon

Faculty Publications from the Center for Plant Science Innovation

Sphingolipid synthesis is tightly regulated in eukaryotes. This regulation in plants ensures sufficient sphingolipids to support growth while limiting the accumulation of sphingolipid metabolites that induce programmed cell death. Serine palmitoyltransferase (SPT) catalyzes the first step in sphingolipid biosynthesis and is considered the primary sphingolipid homeostatic regulatory point. In this report, Arabidopsis (Arabidopsis thaliana) putative SPT regulatory proteins, orosomucoidlike proteins AtORM1 and AtORM2, were found to interact physically with Arabidopsis SPT and to suppress SPT activity when coexpressed with Arabidopsis SPT subunits long-chain base1 (LCB1) and LCB2 and the small subunit of SPT in a yeast (Saccharomyces cerevisiae) SPT-deficient mutant ...


Bacterial Leaf Streak Of Corn Confirmed In Nebraska, Other Corn Belt States August 26, 2016, Tamra Jackson-Ziems, Kevin A. Korus, Tony Adesemoye, Julie Van Meter 2016 University of Nebraska-Lincoln

Bacterial Leaf Streak Of Corn Confirmed In Nebraska, Other Corn Belt States August 26, 2016, Tamra Jackson-Ziems, Kevin A. Korus, Tony Adesemoye, Julie Van Meter

Papers in Plant Pathology

Bacterial leaf streak disease of corn, caused by Xanthomonas vasicola pv. vasculorum, has now been confirmed in Nebraska, as well as in Colorado, Illinois, Iowa, and Kansas. The disease had not been previously identified in the U.S., but had been reported on corn in South Africa. Surveys are currently underway across the Corn Belt to identify the disease distribution. Initial observations and survey results suggest that it may be widely distributed throughout the Corn Belt. Unusual symptoms were first reported on corn samples received by the University of Nebraska Plant and Pest Diagnostic Clinic. Over the last two years ...


Transcriptome Response Of Cassava Leaves Under Natural Shade, Zehong Ding, Yang Zhang, Yi Xiao, Fangfang Liu, Minghui Wang, Xinguang Zhu, Peng Liu, Qi Sun, Wenquan Wang, Ming Peng, Tom Brutnell, Pinghua Li 2016 Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou, Hainan

Transcriptome Response Of Cassava Leaves Under Natural Shade, Zehong Ding, Yang Zhang, Yi Xiao, Fangfang Liu, Minghui Wang, Xinguang Zhu, Peng Liu, Qi Sun, Wenquan Wang, Ming Peng, Tom Brutnell, Pinghua Li

Faculty Publications from the Center for Plant Science Innovation

Cassava is an important staple crop in tropical and sub-tropical areas. As a common farming practice, cassava is usually cultivated intercropping with other crops and subjected to various degrees of shading, which causes reduced productivity. Herein, a comparative transcriptomic analysis was performed on a series of developmental cassava leaves under both full sunlight and natural shade conditions. Gene expression profiles of these two conditions exhibited similar developmental transitions, e.g. genes related to cell wall and basic cellular metabolism were highly expressed in immature leaves, genes involved in lipid metabolism and tetrapyrrole synthesis were highly expressed during the transition stages ...


Validation Of Reference Genes For Robust Qrt-Pcr Gene Expression Analysis In The Rice Blast Fungus Magnaporthe Oryzae, Sarena Che Omar, Michael A. Bentley, Giulia Morieri, Gail M. Preston, Sarah J. Gurr, Richard Wilson (editor) 2016 University of Oxford

Validation Of Reference Genes For Robust Qrt-Pcr Gene Expression Analysis In The Rice Blast Fungus Magnaporthe Oryzae, Sarena Che Omar, Michael A. Bentley, Giulia Morieri, Gail M. Preston, Sarah J. Gurr, Richard Wilson (Editor)

Papers in Plant Pathology

The rice blast fungus causes significant annual harvest losses. It also serves as a genetically- tractable model to study fungal ingress. Whilst pathogenicity determinants have been unmasked and changes in global gene expression described, we know little about Magnaporthe oryzae cell wall remodelling. Our interests, in wall remodelling genes expressed during infection, vegetative growth and under exogenous wall stress, demand robust choice of reference genes for quantitative Real Time-PCR (qRT-PCR) data normalisation. We describe the expression stability of nine candidate reference genes profiled by qRT-PCR with cDNAs derived during asexual germling development, from sexual stage perithecia and from vegetative mycelium ...


Over-Summering Ecology Of The Wheat Curl Mite (Aceria Tosichella Keifer), Anthony J. McMechan 2016 University of Nebraska - Lincoln

Over-Summering Ecology Of The Wheat Curl Mite (Aceria Tosichella Keifer), Anthony J. Mcmechan

Dissertations and Student Research in Entomology

The wheat-mite-virus complex is a consistent and significant threat to winter wheat production in the western Great Plains. This complex consists of three viruses (Wheat streak mosaic virus, Triticum mosaic virus, and Wheat mosaic virus that are transmitted by the wheat curl mite (Aceria tosichella Keifer). Yield impacts from this complex are typically associated with the presence of volunteer wheat that emerges prior to harvest as a result of hail occurring during the heading stages of wheat in early summer. Historical literature on pre-harvest germination has been primarily focused on accelerating breeding programs; however, critical gaps in knowledge exist on ...


Anaerobic Soil Disinfestation: Meta-Analysis And Optimization Of Amendment Carbon Rate And C:N Ratio To Control Key Plant Pathogens And Weeds, Utsala Shrestha 2016 University of Tennessee, Knoxville

Anaerobic Soil Disinfestation: Meta-Analysis And Optimization Of Amendment Carbon Rate And C:N Ratio To Control Key Plant Pathogens And Weeds, Utsala Shrestha

Doctoral Dissertations

Anaerobic soil disinfestation (ASD) is an environmentally friendly and cost effective pre-plant soil treatment technique that allows effective control of soilborne pests by creating anaerobic conditions, particularly for specialty and organic crop production under diverse environmental conditions. In spite of being a proven technique, ASD has to be optimized to fit into local production systems with specific pathogen pressure using locally available amendments for successful implementation on a commercial scale. Our meta-analysis study on soilborne pathogens, plant parasitic nematodes, and weeds validated that ASD is an effective approach to control various soilborne pathogens. This study aims to optimize the carbon ...


Diversity And Virulence Of Soybean Cyst Nematode (Heterodera Glycines Ichinohe) In Nebraska, Kyle C. Broderick 2016 University of Nebraska-Lincoln

Diversity And Virulence Of Soybean Cyst Nematode (Heterodera Glycines Ichinohe) In Nebraska, Kyle C. Broderick

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is one of the most economically important soybean pathogens in the United States. Best management practices are the use of resistant cultivars and crop rotation. Though there are several genetic sources of SCN resistance, most of the SCN-resistant cultivars are derived from a single resistance source (PI 88788). Other states have reported an increase in virulence to PI 88788 due to prolonged use of this resistance. In this thesis, two studies were conducted to characterize the diversity and virulent phenotypes of SCN populations in Nebraska.

The first study assessed the virulent phenotypes of ...


Morphological And Molecular Identification And Characterization Of Dry Bean Fungal Root Rot Pathogens In Zambia, Chikoti Mukuma 2016 University of Nebraska-Lincoln

Morphological And Molecular Identification And Characterization Of Dry Bean Fungal Root Rot Pathogens In Zambia, Chikoti Mukuma

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Dry bean is among the most important food legume crops for direct human consumption in Latin America and Africa. Recently, root and crown rot (RCR) has emerged as an important production constraint. Root and crown rot often involve fungal complexes. Thus, the straw, detached leaf, cup and stem tests were compared on their ability to detect the most common pathogens reported to be associated with RCR: Fusarium solani, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Macrophomina phaseolina.Significant differences (P

Next Generation Sequencing (NGS), conventional Polymerase Chain Reaction (PCR), and classical fungal culturing methods were compared in identification of the ...


Influence Of Application Technology On Foliar Fungicide Efficacy On Cercospora Sojina Infected Soybean, Shawn Alan Butler 2016 University of Tennessee, Knoxville

Influence Of Application Technology On Foliar Fungicide Efficacy On Cercospora Sojina Infected Soybean, Shawn Alan Butler

Masters Theses

Due to the constant concern with off-target contamination and application technology requirements associated with future herbicide-tolerant crops, the use of drift-reduction nozzle technology (DRT) may increase. The primary objective of this research was to evaluate the effects of coarse droplets generated by drift-reduction nozzles on foliar fungicide efficacy and residual in soybean infected by frogeye leaf spot caused by Cercospora sojina. No differences in disease control, soybean yield, spray retention, and residual when applying Quadris Top SB, a premix of azoxystrobin and difenoconazole, using nozzles that produce either a medium or ultra coarse droplet spectrum were determined.

Due to the ...


Timing Of Strobilurin Fungicide For Control Of Top Dieback In Corn, Eric Adee, Stu Duncan 2016 Kansas State University

Timing Of Strobilurin Fungicide For Control Of Top Dieback In Corn, Eric Adee, Stu Duncan

Kansas Agricultural Experiment Station Research Reports

Significant yield losses can result from top dieback (TDB) in dent corn (Zea mays), which is caused by infection by the fungus causing anthracnose (Colletotrichum graminicola). Research is limited on the effectiveness of fungicide application because of the unpredictable nature of the disease. Two field studies were established to assess the timing of fungicide application on TDB, one in Illinois (2010) and the other in Kansas (2015). Fungicide applications at tasseling (VT) and later were effective in reducing the incidence of TDB 20 to 30% and increasing yield 15 to 19 bu/a, or up to 10%, while earlier applications ...


Sudden Death Syndrome And Soybean Planting Date, Eric Adee, C. R. Little, I. A. Ciampitti 2016 Kansas State University

Sudden Death Syndrome And Soybean Planting Date, Eric Adee, C. R. Little, I. A. Ciampitti

Kansas Agricultural Experiment Station Research Reports

The effect of soybean planting date on the severity of sudden death syndrome (SDS) and yield were evaluated in two studies at the Kansas River Valley Experiment Field in 2015. One study was established to promote SDS and the other to minimize SDS. In both studies the severity of SDS was greatest with the earlier planting dates, except for the more tolerant variety. The yield was greatest with the earlier planting date, except for the most susceptible variety. The severity of SDS was not as great as had been observed in previous years.


Interaction Between Seed Treatment And Variety On Sudden Death Syndrome Symptoms And Soybean Yield, Eric Adee 2016 Kansas State University

Interaction Between Seed Treatment And Variety On Sudden Death Syndrome Symptoms And Soybean Yield, Eric Adee

Kansas Agricultural Experiment Station Research Reports

Sudden Death Syndrome (SDS) is a soybean disease that perennially limits yields in the Kansas River Valley (KRV). The presence of soybean cyst nematode (SCN) and saturated soils has been implicated in contributing to the severity of the disease. Selecting varieties with some degree of tolerance to SDS has been the primary cultural practice to reduce yield loss to SDS. Another tool to reduce yield loss to SDS has been made available to growers with the release of ILeVO seed treatment from Bayer CropScience (Research Triangle Park, NC). The potential benefit of ILeVO on varieties with different levels of tolerance ...


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