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Talking Plants: Examining The Role Of Podcasts In Communicating Plant Pathology Knowledge, Melissa Lim, Rebecca Swenson 2021 University of Minnesota

Talking Plants: Examining The Role Of Podcasts In Communicating Plant Pathology Knowledge, Melissa Lim, Rebecca Swenson

Journal of Applied Communications

Extension programs must constantly evaluate communication plans and platforms to determine if they are worth the investment of time, money, and resources. Podcasts are growing in popularity as a communication platform for education and entertainment. With the VARK model of learning styles and core plant pathology concepts as a guide, researchers evaluated the prevalence and type of plant pathology information in podcasts to better understand how podcasts could benefit Extension plant pathology programs. Using keywords related to plant pathology, researchers searched popular mobile podcast listening applications and evaluated relevant podcast episodes using content analysis methods. Results indicated few podcast shows ...


Baseline Sensitivity To Dmi Fungicides In Cercospora Spp. And Corynespora Spp. In Arkansas Soybeans, Evan Buckner 2021 University of Arkansas, Fayetteville

Baseline Sensitivity To Dmi Fungicides In Cercospora Spp. And Corynespora Spp. In Arkansas Soybeans, Evan Buckner

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

Cercospora spp. and Corynespora spp. are two common foliar fungal pathogens in Arkansas amongst other soybean producing areas. Two primary diseases caused by Cercospora spp. are Cercospora Leaf Blight (CLB, caused mainly by C. kikuchii) and Frogeye Leaf Spot (C. sojina). Both diseases affect foliage, and when lesions collapse, leaves may fall prematurely resulting in yield loss. In the specific case of CLB, this is a disease on the rise since 2000, and also causes seed infection reducing seed quality. Target spot is a disease caused by Corynespora cassiicola, and is of less damaging for farmers in larger soybean producing ...


Vegetable Diseases Of Utah, Claudia Nischwitz, Mair Murray, Nick Volesky 2021 Utah State University

Vegetable Diseases Of Utah, Claudia Nischwitz, Mair Murray, Nick Volesky

All Current Publications

This guide provides a list of vegetable crop diseases that have been documented in Utah along with some that are likely to occur. Plant diseases are caused by pathogens such as fungi, bacteria, viruses, and fungal-like organisms. Diagnosing specific plant diseases takes careful observation of signs, symptoms, and sometimes culturing and molecular testing in a laboratory setting.


Mancha Anular Necrótica Y Mancha De Verano Del Césped, Claudia Nischwitz, Kelly Kopp, Jaydee Gunnell, Kent Evans, Erin Frank, Celina Wille 2021 Utah State University

Mancha Anular Necrótica Y Mancha De Verano Del Césped, Claudia Nischwitz, Kelly Kopp, Jaydee Gunnell, Kent Evans, Erin Frank, Celina Wille

All Current Publications

La mancha anular necrótica y mancha de verano del césped son enfermedades fúngicas que infectan los pastos de estación fría y causan síntomas similares. Los síntomas comienzan como un crecimiento deficiente y marchitez de las hojas, que se convierten en parches marrones obvios que varían de 1 a 3 pies de diámetro. Debido a los síntomas similares que se observan en la infección por ambos patógenos, se necesita un microscopio para diagnosticar adecuadamente cada enfermedad. El manejo de ambas enfermedades puede ser difícil y frecuentemente requiere una combinación de técnicas de control cultural, químico y varietal.


Gmnf-Yc4-2 Increases Protein, Exhibits Broad Disease Resistance And Expedites Maturity In Soybean, Seth O’Conner, Wenguang Zheng, Mingsheng Qi, Yuba Kandel, Robert Fuller, Steven A. Whitham, Ling Li 2021 Mississippi State University

Gmnf-Yc4-2 Increases Protein, Exhibits Broad Disease Resistance And Expedites Maturity In Soybean, Seth O’Conner, Wenguang Zheng, Mingsheng Qi, Yuba Kandel, Robert Fuller, Steven A. Whitham, Ling Li

Plant Pathology and Microbiology Publications

The NF-Y gene family is a highly conserved set of transcription factors. The functional transcription factor complex is made up of a trimer between NF-YA, NF-YB, and NF-YC proteins. While mammals typically have one gene for each subunit, plants often have multigene families for each subunit which contributes to a wide variety of combinations and functions. Soybean plants with an overexpression of a particular NF-YC isoform GmNF-YC4-2 (Glyma.04g196200) in soybean cultivar Williams 82, had a lower amount of starch in its leaves, a higher amount of protein in its seeds, and increased broad disease resistance for bacterial, viral, and ...


Dynamic Interplay Between The Co-Opted Fis1 Mitochondrial Fission Protein And Membrane Contact Site Proteins In Supporting Tombusvirus Replication, Wenwu Lin, Zhike Feng, K. Reddisiva Prasanth, Yuyan Liu, Peter D. Nagy 2021 University of Kentucky

Dynamic Interplay Between The Co-Opted Fis1 Mitochondrial Fission Protein And Membrane Contact Site Proteins In Supporting Tombusvirus Replication, Wenwu Lin, Zhike Feng, K. Reddisiva Prasanth, Yuyan Liu, Peter D. Nagy

Plant Pathology Faculty Publications

Plus-stranded RNA viruses have limited coding capacity and have to co-opt numerous pro-viral host factors to support their replication. Many of the co-opted host factors support the biogenesis of the viral replication compartments and the formation of viral replicase complexes on subverted subcellular membrane surfaces. Tomato bushy stunt virus (TBSV) exploits peroxisomal membranes, whereas the closely-related carnation Italian ringspot virus (CIRV) hijacks the outer membranes of mitochondria. How these organellar membranes can be recruited into pro-viral roles is not completely understood. Here, we show that the highly conserved Fis1 mitochondrial fission protein is co-opted by both TBSV and CIRV via ...


Non-Transgenic Crispr-Mediated Knockout Of Entire Ergot Alkaloid Gene Clusters In Slow-Growing Asexual Polyploid Fungi, Simona Florea, Jolanta Jaromczyk, Christopher L. Schardl 2021 University of Kentucky

Non-Transgenic Crispr-Mediated Knockout Of Entire Ergot Alkaloid Gene Clusters In Slow-Growing Asexual Polyploid Fungi, Simona Florea, Jolanta Jaromczyk, Christopher L. Schardl

Computer Science Faculty Publications

The Epichloë species of fungi include seed-borne symbionts (endophytes) of cool-season grasses that enhance plant fitness, although some also produce alkaloids that are toxic to livestock. Selected or mutated toxin-free endophytes can be introduced into forage cultivars for improved livestock performance. Long-read genome sequencing revealed clusters of ergot alkaloid biosynthesis (EAS) genes in Epichloë coenophiala strain e19 from tall fescue (Lolium arundinaceum) and Epichloë hybrida Lp1 from perennial ryegrass (Lolium perenne). The two homeologous clusters in E. coenophiala—a triploid hybrid species—were 196 kb (EAS1) and 75 kb (EAS2), and the E. hybrida EAS cluster was 83 ...


Direct Agroinoculation Of Maize Seedlings By Injection With Recombinant Foxtail Mosaic Virus And Sugarcane Mosaic Virus Infectious Clones, Bliss M. Beernink, Katerina L. Holan, Ryan R. Lappe, Steven A. Whitham 2021 Iowa State University

Direct Agroinoculation Of Maize Seedlings By Injection With Recombinant Foxtail Mosaic Virus And Sugarcane Mosaic Virus Infectious Clones, Bliss M. Beernink, Katerina L. Holan, Ryan R. Lappe, Steven A. Whitham

Plant Pathology and Microbiology Publications

Agrobacterium-based inoculation approaches are widely used for introducing viral vectors into plant tissues. This study details a protocol for the injection of maize seedlings near meristematic tissue with Agrobacterium carrying a viral vector. Recombinant foxtail mosaic virus (FoMV) clones engineered for gene silencing and gene expression were used to optimize this method, and its use was expanded to include a recombinant sugarcane mosaic virus (SCMV) engineered for gene expression. Gene fragments or coding sequences of interest are inserted into a modified, infectious viral genome that has been cloned into the binary T-DNA plasmid vector pCAMBIA1380. The resulting plasmid constructs ...


Quantitative Proteomics Reveals Extensive Lysine Ubiquitination In The Arabidopsis Root Proteome And Uncovers Novel Transcription Factor Stability States, Gaoyuan Song, Damilola Olatunji, Christian Montes, Natalie M. Clark, Yunting Pu, Dior R. Kelley, Justin W. Walley 2021 Iowa State University

Quantitative Proteomics Reveals Extensive Lysine Ubiquitination In The Arabidopsis Root Proteome And Uncovers Novel Transcription Factor Stability States, Gaoyuan Song, Damilola Olatunji, Christian Montes, Natalie M. Clark, Yunting Pu, Dior R. Kelley, Justin W. Walley

Genetics, Development and Cell Biology Publications

Protein activity, abundance, and stability can be regulated by posttranslational modification including ubiquitination. Ubiquitination is conserved among eukaryotes and plays a central role in modulating cellular function and yet we lack comprehensive catalogs of proteins that are modified by ubiquitin in plants. In this study, we describe an antibody-based approach to enrich peptides containing the di-glycine (diGly) remnant of ubiquitin and coupled that with isobaric labeling to enable quantification, from up to 16-multiplexed samples, for plant tissues. Collectively, we identified 7,130 diGly-modified lysine residues sites arising from 3,178 proteins in Arabidopsis primary roots. These data include ubiquitin proteasome ...


Introduction To Special Issue Of Plant Virus Emergence, Michael M. Goodin, Jeanmarie Verchot 2021 University of Kentucky

Introduction To Special Issue Of Plant Virus Emergence, Michael M. Goodin, Jeanmarie Verchot

Plant Pathology Faculty Publications

No abstract provided.


72-H Diurnal Rna-Seq Analysis Of Fully Expanded Third Leaves From Maize, Sorghum, And Foxtail Millet At 3-H Resolution, Xianjun Lai, Claire Bendix, Yan Zhang, James Schnable, Frank G. Harmon 2021 University of Nebraska - Lincoln & Xichang University

72-H Diurnal Rna-Seq Analysis Of Fully Expanded Third Leaves From Maize, Sorghum, And Foxtail Millet At 3-H Resolution, Xianjun Lai, Claire Bendix, Yan Zhang, James Schnable, Frank G. Harmon

Faculty Publications from the Center for Plant Science Innovation

Objectives: The purpose of this data set is to capture the complete diurnal (i.e., daily) transcriptome of fully expanded third leaves from the C4 panacoid grasses sorghum (Sorghum bicolor), maize (Zea mays), and foxtail millet (Setaria italica) with RNA-seq transcriptome profiling. These data are the cornerstone of a larger project that examined the conservation and divergence of gene expression networks within these crop plants. This data set focuses on tem- poral changes in gene expression to identify the network architecture responsible for daily regulation of plant growth and metabolic activities. The power of this data set is fine temporal ...


Widespread Mortality Of Trembling Aspen (Populus Tremuloides) Throughout Interior Alaskan Boreal Forests Resulting From A Novel Canker Disease, Roger W. Ruess, Loretta M. Winton, Gerard C. Adams 2021 University of Alaska, Fairbanks

Widespread Mortality Of Trembling Aspen (Populus Tremuloides) Throughout Interior Alaskan Boreal Forests Resulting From A Novel Canker Disease, Roger W. Ruess, Loretta M. Winton, Gerard C. Adams

Papers in Plant Pathology

Over the past several decades, growth declines and mortality of trembling aspen throughout western Canada and the United States have been linked to drought, often interacting with outbreaks of insects and fungal pathogens, resulting in a “sudden aspen decline” throughout much of aspen’s range. In 2015, we noticed an aggressive fungal canker causing widespread mortality of aspen throughout interior Alaska and initiated a study to quantify potential drivers for the incidence, virulence, and distribution of the disease. Stand-level infection rates among 88 study sites distributed across 6 Alaska ecoregions ranged from <1 to 69%, with the proportion of trees with canker that were dead averaging 70% across all sites. The disease is most prevalent north of the Alaska Range within the Tanana Kuskokwim ecoregion. Modeling canker probability as a function of ecoregion, stand structure, landscape position, and climate revealed that smaller-diameter trees in older stands with greater aspen basal area have the highest canker incidence and mortality, while younger trees in younger stands appear virtually immune to the disease. Sites with higher summer vapor pressure deficits had significantly higher levels of canker infection and mortality. We believe the combined effects of this novel fungal canker pathogen, drought, and the persistent aspen leaf miner outbreak are triggering feedbacks between carbon starvation and hydraulic failure that are ultimately driving widespread mortality. Warmer early-season temperatures and prolonged late summer drought are leading to larger and more severe wildfires throughout interior Alaska that are favoring a shift from black spruce to forests dominated by Alaska paper birch and aspen. Widespread aspen mortality fostered by this rapidly spreading pathogen has significant implications for successional dynamics, ecosystem function, and feedbacks to disturbance regimes, particularly on sites too dry for Alaska paper birch.


Impact Of Fungicide On Wheat, G. F. Sassenrath, H. Zhao, X. Lin 2021 Kansas State University

Impact Of Fungicide On Wheat, G. F. Sassenrath, H. Zhao, X. Lin

Kansas Agricultural Experiment Station Research Reports

This is a report of research to test the impact of fungicide and management on wheat yield and quality. Fusarium head blight (FHB), or scab, is a persistent problem in wheat production, especially in high rainfall areas such as eastern Kansas. Two cultivars of winter wheat varying in FHB sensitivity (Everest, moderately resistant, and KanMark, susceptible) were tested for control of FHB using fungicide treatments made to the seed prior to planting or to the wheat plant at heading, in tilled or no-tilled management. The wet spring of 2020 resulted in high FHB pressure, but dry conditions at harvest re ...


Robotic Agricultural Instrument For Automated Extraction Of Nematode Cysts And Eggs From Soil To Improve Integrated Pest Management, Christopher M. Legner, Gregory L. Tylka, Santosh Pandey 2021 Iowa State University

Robotic Agricultural Instrument For Automated Extraction Of Nematode Cysts And Eggs From Soil To Improve Integrated Pest Management, Christopher M. Legner, Gregory L. Tylka, Santosh Pandey

Plant Pathology and Microbiology Publications

Soybeans are an important crop for global food security. Every year, soybean yields are reduced by numerous soybean diseases, particularly the soybean cyst nematode (SCN). It is difficult to visually identify the presence of SCN in the field, let alone its population densities or numbers, as there are no obvious aboveground disease symptoms. The only definitive way to assess SCN population densities is to directly extract the SCN cysts from soil and then extract the eggs from cysts and count them. Extraction is typically conducted in commercial soil analysis laboratories and university plant diagnostic clinics and involves repeated steps of ...


Known Distribution Of The Soybean Cyst Nematode, Heterodera Glycines, In The United States And Canada In 2020, Gregory L. Tylka, Christopher C. Marett 2021 Iowa State University

Known Distribution Of The Soybean Cyst Nematode, Heterodera Glycines, In The United States And Canada In 2020, Gregory L. Tylka, Christopher C. Marett

Plant Pathology and Microbiology Publications

In the United States and Canada, the most damaging pathogen of soybean, Glycine max, is the soybean cyst nematode (SCN), Heterodera glycines. Plant health professionals working for universities and state and provincial departments of agriculture in the United States and Canada are queried periodically about counties and rural municipalities that are newly known to be infested with SCN in their states and provinces. Such a census was conducted in 2020, and the results were compared with results of the most recent survey, published in 2017. Between 2017 and 2020, 55 new SCN-infested counties were reported from 11 U.S. states ...


Biological Control And Microbial Ecology Draft Genome Sequence Data Of Glutamicibacter Sp. Fbe-19, A Bacterium Antagonistic To The Plant Pathogen Erwinia Tracheiphila, Benzhong Fu, Olakunle Olawole, Gwyn A. Beattie 2021 Iowa State University

Biological Control And Microbial Ecology Draft Genome Sequence Data Of Glutamicibacter Sp. Fbe-19, A Bacterium Antagonistic To The Plant Pathogen Erwinia Tracheiphila, Benzhong Fu, Olakunle Olawole, Gwyn A. Beattie

Plant Pathology and Microbiology Publications

Glutamicibacter sp.FBE-19 was isolated based on its strong antagonism to the cucurbit bacterial blight pathogen Erwinia tracheiphila on plates. Members of the Glutamicibacter genus can promote plant growth under saline conditions and antagonize fungi on plates via chitinolytic activity; however, their production of antibacterial compounds has not been examined. Here, we report the genome sequence of strain FBE-19. The genome is 3.85 Mbp with a G+C content of 60.1% and comprises 3,791 genes. Genes that may contribute to its antagonistic activity include genes for the secondary metabolites stenothricin, salinosporamide A, a second β-lactone compound, and ...


A Sugarcane Mosaic Virus Vector For Rapid In Planta Screening Of Proteins That Inhibit The Growth Of Insect Herbivores, Seung Ho Chung, Mahdiyeh Bigham, Ryan R. Lappe, Barry Chan, Ugrappa Nagalakshmi, Steven A. Whitham, Savithramma P. Dinesh-Kumar, Georg Jander 2021 Boyce Thompson Institute for Plant Research

A Sugarcane Mosaic Virus Vector For Rapid In Planta Screening Of Proteins That Inhibit The Growth Of Insect Herbivores, Seung Ho Chung, Mahdiyeh Bigham, Ryan R. Lappe, Barry Chan, Ugrappa Nagalakshmi, Steven A. Whitham, Savithramma P. Dinesh-Kumar, Georg Jander

Plant Pathology and Microbiology Publications

Spodoptera frugiperda (fall armyworm) is a notorious pest that threatens maize production worldwide. Current control measures involve the use of chemical insecticides and transgenic maize expressing Bacillus thuringiensis (Bt) toxins. Although additional transgenes have confirmed insecticidal activity, limited research has been conducted in maize, at least partially due to the technical difficulty of maize transformation. Here, we describe implementation of a sugarcane mosaic virus (SCMV) vector for rapidly testing the efficacy of both endogenous maize genes and heterologous genes from other organisms for the control of S. frugiperda in maize. Four categories of proteins were tested using the SCMV vector ...


Assaying Bacillus Amyloliquefaciens 1ba A=And 1d3 For Utilization Of Petroleum Hydrocarbons And Biosurfactant Production, Pavan Kulkarni 2021 South Dakota State University

Assaying Bacillus Amyloliquefaciens 1ba A=And 1d3 For Utilization Of Petroleum Hydrocarbons And Biosurfactant Production, Pavan Kulkarni

Electronic Theses and Dissertations

Several Bacillus amyloliquefaciens isolates obtained from wheat residue have been studied as biological agents to control wheat diseases. Notable traits of isolates 1BA and 1D3 include growth with high salt (10% NaCl), at temperatures up to 50°C and over a wide pH range. BIOLOG Gen III plates were used to study and further characterize Bacillus amyloliquefaciens 1BA and 1D3. Both isolates showed tolerance to high salt concentration supporting previous studies. They also grew in the presence of lithium chloride, potassium tellurite, and sodium bromate. Both isolates grew at pH 5, with almost identical carbon source utilization fingerprints. However, D-serine ...


Use Of Foliar Chemical Treatments To Induce Disease Resistance In Rhododendrons Inoculated With Phytophthora Ramorum, Craig Ramsey, Paul C. Freebury, Debra H. Newman, Wolfgang Schweigkofler, Leland J. Cseke, Steven E. Newman 2021 Retired– USDA Fort Collins

Use Of Foliar Chemical Treatments To Induce Disease Resistance In Rhododendrons Inoculated With Phytophthora Ramorum, Craig Ramsey, Paul C. Freebury, Debra H. Newman, Wolfgang Schweigkofler, Leland J. Cseke, Steven E. Newman

Natural Sciences and Mathematics | Faculty Scholarship

A field study was conducted at the National Ornamental Research Site at Dominican University California (NORS-DUC). The study goal was to evaluate three chemical inducers applied as foliar treatments for controlling Phytophthora ramorum, on Rhododendron x ‘Cunningham’s White’ nursery plants. The inducers were chlorine dioxide (ElectroBiocide), hydrogen peroxide (OxiDate 2.0), and acibenzolar-s methyl (Actigard). Water samples from the electrostatic sprayer were measured for three physicochemical water properties. Visual assessment of plant foliage, based on the Horsfall- Barratt scale, was conducted at three and five months after chemical treatments. Foliar fluorescence (Fv/Fm) was measured over three dates. The ...


Evaluation Of Corn Agronomic Management Practices Following A Rye Cover Crop, Daniel John Quinn 2021 University of Kentucky

Evaluation Of Corn Agronomic Management Practices Following A Rye Cover Crop, Daniel John Quinn

Theses and Dissertations--Plant and Soil Sciences

Fall implementation of a rye (Secale cereale L.) cover crop (RCC) prior to spring corn (Zea mays L.) planting is a management practice used to improve soil conservation, water quality, and limit herbicide dependence. However, corn growth and yield following a RCC is often reduced due to early-season nitrogen (N) stress and decreased plant emergence, which can limit RCC adoption. The objective(s) of this research were to evaluate corn agronomic management practices (e.g., N and seeding rate management, in-furrow (IF) starter use) following a RCC and determine which management practices can be used to limit corn stress following ...


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