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Transcriptome Response Of Cassava Leaves Under Natural Shade, Zehong Ding, Yang Zhang, Yi Xiao, Fangfang Liu, Minghui Wang, Xinguang Zhu, Peng Liu, Qi Sun, Wenquan Wang, Ming Peng, Tom Brutnell, Pinghua Li 2016 Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou, Hainan

Transcriptome Response Of Cassava Leaves Under Natural Shade, Zehong Ding, Yang Zhang, Yi Xiao, Fangfang Liu, Minghui Wang, Xinguang Zhu, Peng Liu, Qi Sun, Wenquan Wang, Ming Peng, Tom Brutnell, Pinghua Li

Faculty Publications from the Center for Plant Science Innovation

Cassava is an important staple crop in tropical and sub-tropical areas. As a common farming practice, cassava is usually cultivated intercropping with other crops and subjected to various degrees of shading, which causes reduced productivity. Herein, a comparative transcriptomic analysis was performed on a series of developmental cassava leaves under both full sunlight and natural shade conditions. Gene expression profiles of these two conditions exhibited similar developmental transitions, e.g. genes related to cell wall and basic cellular metabolism were highly expressed in immature leaves, genes involved in lipid metabolism and tetrapyrrole synthesis were highly expressed during the transition stages ...


Over-Summering Ecology Of The Wheat Curl Mite (Aceria Tosichella Keifer), Anthony J. McMechan 2016 University of Nebraska - Lincoln

Over-Summering Ecology Of The Wheat Curl Mite (Aceria Tosichella Keifer), Anthony J. Mcmechan

Dissertations and Student Research in Entomology

The wheat-mite-virus complex is a consistent and significant threat to winter wheat production in the western Great Plains. This complex consists of three viruses (Wheat streak mosaic virus, Triticum mosaic virus, and Wheat mosaic virus that are transmitted by the wheat curl mite (Aceria tosichella Keifer). Yield impacts from this complex are typically associated with the presence of volunteer wheat that emerges prior to harvest as a result of hail occurring during the heading stages of wheat in early summer. Historical literature on pre-harvest germination has been primarily focused on accelerating breeding programs; however, critical gaps in knowledge exist on ...


Diversity And Virulence Of Soybean Cyst Nematode (Heterodera Glycines Ichinohe) In Nebraska, Kyle C. Broderick 2016 University of Nebraska-Lincoln

Diversity And Virulence Of Soybean Cyst Nematode (Heterodera Glycines Ichinohe) In Nebraska, Kyle C. Broderick

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is one of the most economically important soybean pathogens in the United States. Best management practices are the use of resistant cultivars and crop rotation. Though there are several genetic sources of SCN resistance, most of the SCN-resistant cultivars are derived from a single resistance source (PI 88788). Other states have reported an increase in virulence to PI 88788 due to prolonged use of this resistance. In this thesis, two studies were conducted to characterize the diversity and virulent phenotypes of SCN populations in Nebraska.

The first study assessed the virulent phenotypes of ...


Morphological And Molecular Identification And Characterization Of Dry Bean Fungal Root Rot Pathogens In Zambia, Chikoti Mukuma 2016 University of Nebraska-Lincoln

Morphological And Molecular Identification And Characterization Of Dry Bean Fungal Root Rot Pathogens In Zambia, Chikoti Mukuma

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Dry bean is among the most important food legume crops for direct human consumption in Latin America and Africa. Recently, root and crown rot (RCR) has emerged as an important production constraint. Root and crown rot often involve fungal complexes. Thus, the straw, detached leaf, cup and stem tests were compared on their ability to detect the most common pathogens reported to be associated with RCR: Fusarium solani, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Macrophomina phaseolina.Significant differences (P

Next Generation Sequencing (NGS), conventional Polymerase Chain Reaction (PCR), and classical fungal culturing methods were compared in identification of the ...


Influence Of Application Technology On Foliar Fungicide Efficacy On Cercospora Sojina Infected Soybean, Shawn Alan Butler 2016 University of Tennessee, Knoxville

Influence Of Application Technology On Foliar Fungicide Efficacy On Cercospora Sojina Infected Soybean, Shawn Alan Butler

Masters Theses

Due to the constant concern with off-target contamination and application technology requirements associated with future herbicide-tolerant crops, the use of drift-reduction nozzle technology (DRT) may increase. The primary objective of this research was to evaluate the effects of coarse droplets generated by drift-reduction nozzles on foliar fungicide efficacy and residual in soybean infected by frogeye leaf spot caused by Cercospora sojina. No differences in disease control, soybean yield, spray retention, and residual when applying Quadris Top SB, a premix of azoxystrobin and difenoconazole, using nozzles that produce either a medium or ultra coarse droplet spectrum were determined.

Due to the ...


Anaerobic Soil Disinfestation: Meta-Analysis And Optimization Of Amendment Carbon Rate And C:N Ratio To Control Key Plant Pathogens And Weeds, Utsala Shrestha 2016 University of Tennessee, Knoxville

Anaerobic Soil Disinfestation: Meta-Analysis And Optimization Of Amendment Carbon Rate And C:N Ratio To Control Key Plant Pathogens And Weeds, Utsala Shrestha

Doctoral Dissertations

Anaerobic soil disinfestation (ASD) is an environmentally friendly and cost effective pre-plant soil treatment technique that allows effective control of soilborne pests by creating anaerobic conditions, particularly for specialty and organic crop production under diverse environmental conditions. In spite of being a proven technique, ASD has to be optimized to fit into local production systems with specific pathogen pressure using locally available amendments for successful implementation on a commercial scale. Our meta-analysis study on soilborne pathogens, plant parasitic nematodes, and weeds validated that ASD is an effective approach to control various soilborne pathogens. This study aims to optimize the carbon ...


Timing Of Strobilurin Fungicide For Control Of Top Dieback In Corn, Eric Adee, Stu Duncan 2016 Kansas State University

Timing Of Strobilurin Fungicide For Control Of Top Dieback In Corn, Eric Adee, Stu Duncan

Kansas Agricultural Experiment Station Research Reports

Significant yield losses can result from top dieback (TDB) in dent corn (Zea mays), which is caused by infection by the fungus causing anthracnose (Colletotrichum graminicola). Research is limited on the effectiveness of fungicide application because of the unpredictable nature of the disease. Two field studies were established to assess the timing of fungicide application on TDB, one in Illinois (2010) and the other in Kansas (2015). Fungicide applications at tasseling (VT) and later were effective in reducing the incidence of TDB 20 to 30% and increasing yield 15 to 19 bu/a, or up to 10%, while earlier applications ...


Sudden Death Syndrome And Soybean Planting Date, Eric Adee, C. R. Little, I. A. Ciampitti 2016 Kansas State University

Sudden Death Syndrome And Soybean Planting Date, Eric Adee, C. R. Little, I. A. Ciampitti

Kansas Agricultural Experiment Station Research Reports

The effect of soybean planting date on the severity of sudden death syndrome (SDS) and yield were evaluated in two studies at the Kansas River Valley Experiment Field in 2015. One study was established to promote SDS and the other to minimize SDS. In both studies the severity of SDS was greatest with the earlier planting dates, except for the more tolerant variety. The yield was greatest with the earlier planting date, except for the most susceptible variety. The severity of SDS was not as great as had been observed in previous years.


Interaction Between Seed Treatment And Variety On Sudden Death Syndrome Symptoms And Soybean Yield, Eric Adee 2016 Kansas State University

Interaction Between Seed Treatment And Variety On Sudden Death Syndrome Symptoms And Soybean Yield, Eric Adee

Kansas Agricultural Experiment Station Research Reports

Sudden Death Syndrome (SDS) is a soybean disease that perennially limits yields in the Kansas River Valley (KRV). The presence of soybean cyst nematode (SCN) and saturated soils has been implicated in contributing to the severity of the disease. Selecting varieties with some degree of tolerance to SDS has been the primary cultural practice to reduce yield loss to SDS. Another tool to reduce yield loss to SDS has been made available to growers with the release of ILeVO seed treatment from Bayer CropScience (Research Triangle Park, NC). The potential benefit of ILeVO on varieties with different levels of tolerance ...


Corn Following Corn In 2008, Mahdi Al-Kaisi, Roger W. Elmore, Antonio P. Mallarino, Palle Pedersen, Alison E. Robertson, John E. Sawyer, Jon J. Tollefson 2016 Iowa State University

Corn Following Corn In 2008, Mahdi Al-Kaisi, Roger W. Elmore, Antonio P. Mallarino, Palle Pedersen, Alison E. Robertson, John E. Sawyer, Jon J. Tollefson

John E. Sawyer

Corn following corn is in rough shape in areas across Iowa. Many wonder what is happening. The crop’s condition in general is not normal for this time of year. For example, last Sunday the USDA rated this year’s Iowa corn crop as 54 percent in ‘Good’ to ‘Excellent’ condition and 15 percent is ‘Poor’ or ‘Very Poor.’ Last year 72 percent was rated ‘Good’ to ‘Excellent’ and 5 percent was in ‘Poor’ or ‘Very Poor’ condition during the same week. Average plant height as of Sunday was 24 inches compared to 40 inches at end of the same ...


Corn Field Guide, Lori Abendroth, Roger Elmore, Robert G. Hartzler, Clarke McGrath, Daren S. Mueller, Gary P. Munkvold, Richard Pope, Marlin E. Rice, Alison E. Robertson, John E. Sawyer, Kristine J. P. Schaefer, Jon James Tollefson, Gregory L. Tylka 2016 Iowa State University

Corn Field Guide, Lori Abendroth, Roger Elmore, Robert G. Hartzler, Clarke Mcgrath, Daren S. Mueller, Gary P. Munkvold, Richard Pope, Marlin E. Rice, Alison E. Robertson, John E. Sawyer, Kristine J. P. Schaefer, Jon James Tollefson, Gregory L. Tylka

John E. Sawyer

A reference for identifying diseases, insect pests, and disorders of corn.


Development Of Cold Hardy, Large Patch Resistant Zoysiagrass Cultivars For The Transition Zone, Mingying Xiang, J. Fry, M. Kennelly 2016 Kansas State University, Manhattan

Development Of Cold Hardy, Large Patch Resistant Zoysiagrass Cultivars For The Transition Zone, Mingying Xiang, J. Fry, M. Kennelly

Kansas Agricultural Experiment Station Research Reports

TAES 5645, a Z. japonica genotype that exhibited resistance to large patch in preliminary studies, was used as a breeding parent and crossed with 22 cold hardy zoysiagrasses, resulting in 985 progeny. These progeny were evaluated for cold hardiness and agronomic traits (establishment rate, overall quality, spring greenup, leaf texture, and genetic color) in Manhattan, KS; West Lafayette, IN; and Dallas, TX; from 2012 to 2014. From this work, 60 progeny were identified for further evaluation in larger plots. During the 2015-2016 establishment year, experimental line 6102-47 showed good vigor and spring green up rate, which is comparable to ‘Meyer ...


Preventative Control Of Brown Patch With Select Fungicides, E. Alderman, J. Reeves, J. Hoyle 2016 Kansas State University

Preventative Control Of Brown Patch With Select Fungicides, E. Alderman, J. Reeves, J. Hoyle

Kansas Agricultural Experiment Station Research Reports

Preventative applications of Velista and Heritage Action fungicides will decrease the percentage of brown patch observed in a perennial stand of tall fescue.


Comparative Genomics, Transcriptomics, And Physiology Distinguish Symbiotic From Free-Living Chlorella Strains, Cristian F. Quispe, Olivia Sonderman, Maya Khasin, Wayne R. Riekhof, James Van Etten, Kenneth Nickerson 2016 University of Nebraska-Lincoln

Comparative Genomics, Transcriptomics, And Physiology Distinguish Symbiotic From Free-Living Chlorella Strains, Cristian F. Quispe, Olivia Sonderman, Maya Khasin, Wayne R. Riekhof, James Van Etten, Kenneth Nickerson

Kenneth Nickerson Papers

Most animal–microbe symbiotic interactions must be advantageous to the host and provide nutritional benefits to the endosymbiont. When the host provides nutrients, it can gain the capacity to control the interaction, promote self-growth, and increase its fitness. Chlorella-like green algae engage in symbiotic relationships with certain protozoans, a partnership that significantly impacts the physiology of both organisms. Consequently, it is often challenging to grow axenic Chlorella cultures after isolation from the host because they are nutrient fastidious and often susceptible to virus infection. We hypothesize that the establishment of a symbiotic relationship resulted in natural selection for nutritional and ...


Rna-Seq Based Analysis Of Population Structure Within The Maize Inbred B73, Zhikai Liang, James C. Schnable 2016 University of Nebraska-Lincoln

Rna-Seq Based Analysis Of Population Structure Within The Maize Inbred B73, Zhikai Liang, James C. Schnable

Faculty Publications from the Center for Plant Science Innovation

Recent reports have shown than many identically named genetic lines used in research around the world actually contain large amounts of uncharacterized genetic variation as a result of cross contamination of stocks, unintentional crossing, residual heterozygosity within original stocks, or de novo mutation. 27 public, large scale, RNA-seq datasets from 20 independent research groups around the world were used to assess variation within the maize (Zea mays ssp. mays) inbred B73, a four decade old variety which served as the reference genotype for the original maize genome sequencing project and is widely used in genetic, genomic, and phenotypic research. Several ...


Exploration Of Chemical And Biological Management Strategies For Diaphorina Citri The Primary Vector Of Candidatus Liberibacter Asiaticus, Gretta Marie Sharp 2016 University of Texas at Tyler

Exploration Of Chemical And Biological Management Strategies For Diaphorina Citri The Primary Vector Of Candidatus Liberibacter Asiaticus, Gretta Marie Sharp

Biology Theses

Diaphorina citri (Hemipteran: Psyllidae), the Asian citrus psyllid, is a phloem-feeding, invasive species endemic to southern Asia that was first identified in the United States in Florida in 1998. Since introduction, D. citri has spread across the major citrus growing regions of the United States. This is of great concern to the citrus industry because D. citri is the primary vector of Candidatus Liberibacter asiaticus the causal agent of Haunglongbing (HLB). Haunglongbing is the most economically devastating disease of citrus causing an estimated 8.92 billion dollars lost in revenue and 4.62 billion dollars lost in gross domestic product ...


A Brief History Of Corn: Looking Back To Move Forward, Jon Derek Pruitt 2016 University of Nebraska-Lincoln

A Brief History Of Corn: Looking Back To Move Forward, Jon Derek Pruitt

Doctoral Documents from Doctor of Plant Health Program

Maize was domesticated from teosinte in Mexico some 7,000 to 10,000 years ago and quickly spread through the Americas. It has become one of the most important crops at a local and global level. Two types, Northern Flint corn and Southern Dent corns provided the basis of the genetic background of modern maize hybrids. The development of hybrids, first double-cross and later single-cross hybrids, along with a transition to high input farming provided huge yield increases, which have continued to improve with improving technology.

Increase in maize production also caused a rise in Western corn Rootworm (Diabrotica virgifera ...


Evaluation Of Fungicide Efficacy Against Cercospora Sojina And Selection For Qoi-Fungicide Resistance In Soybean, Alicia Mercedes Cochran 2016 University of Tennessee - Knoxville

Evaluation Of Fungicide Efficacy Against Cercospora Sojina And Selection For Qoi-Fungicide Resistance In Soybean, Alicia Mercedes Cochran

Masters Theses

Frogeye leaf spot (FLS) of soybean is caused by Cercospora sojina. In 2010, resistance to the quinone outside inhibitor (QoI) fungicides was reported. Since then, evaluating FLS for QoI-resistance has been of particular interest in Tennessee and other soybean-producing states. In order to determine alternative fungicide options, fungicides with solo and combination mode of action were tested in 2013-2014. The objectives were,1) to evaluate fungicide efficacy for disease control and yield protection, and 2) evaluate selection pressure for QoI-resistance. Treatments included the following fungicide groups: QoI, DMI, MBC, Chlorothalonil, QoI+DMI, SDHI+QoI, SDHI+QoI+DMI, and DMI+MBC ...


Microbe-Id: An Open Source Toolbox For Microbial Genotyping And Species Identification, Javier F. Tabima, Sydney E. Everhart, Meredith M. Larsen, Alexaandra J. Weisberg, Zhian N. Kamvar, Mathew A. Tancos, Christine D. Smart, Jeff H. Chang, Niklaus J. Grünwald 2016 Oregon State University

Microbe-Id: An Open Source Toolbox For Microbial Genotyping And Species Identification, Javier F. Tabima, Sydney E. Everhart, Meredith M. Larsen, Alexaandra J. Weisberg, Zhian N. Kamvar, Mathew A. Tancos, Christine D. Smart, Jeff H. Chang, Niklaus J. Grünwald

Papers in Plant Pathology

Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by ...


Managing Drought Stress In California Agricultural Systems, Gregory D. Brittain Jr. 2016 University of Nebraska-Lincoln

Managing Drought Stress In California Agricultural Systems, Gregory D. Brittain Jr.

Doctoral Documents from Doctor of Plant Health Program

California is currently facing a historic drought, and this has led many farmers in the state to severely cut back on irrigation. Optimal use of water for irrigation requires a comprehensive understanding of how plants respond physiologically to water stress (Chapter 1). By monitoring water requirements in crops and managing irrigation to meet those requirements, growers can significantly reduce water use (Chapter 2). This can be done through improving application efficiency of irrigation technology as well as increasing the water use efficiency of the crops themselves. Deficit irrigation practices can be used to manipulate the physiology of water use in ...


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