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Development Of Molecular Tools For Identification Of Prairie Terrestrial And Wetland Algae, Esther Mundembo Kapinga 2017 University of Nebraska at Omaha

Development Of Molecular Tools For Identification Of Prairie Terrestrial And Wetland Algae, Esther Mundembo Kapinga

Student Research and Creative Activity Fair

Algae are a diverse group of photosynthetic protists. Green algae are typically unicellular, though some may occur as multicellular colonies, and they are found across a wide range of habitats, including marine, freshwater, and terrestrial environments. As photosynthetic producers and sometimes symbionts, algae can occupy important roles in an ecosystem; in other circumstances they can have negative impacts on environmental quality. Estimations of the number of green algal species range from eight thousand to over fifteen thousand. The identification of individual algal species can be problematic due to their small size and typically simple cell anatomy. For this reason, molecular ...


Small Scale Heterogeneity In Vegetation Structure In A Patch-Burn-Grazing Landscape, Eric G. Behrens 2017 ebehrens@unomaha.edu

Small Scale Heterogeneity In Vegetation Structure In A Patch-Burn-Grazing Landscape, Eric G. Behrens

Student Research and Creative Activity Fair

Disturbances are drivers of spatial change in the plant communities of the tallgrass prairie ecosystem. Fire and large herbivore grazing are prevalent disturbances that maintain the vegetation structure and create spatial heterogeneity in vegetation at various scales. The fire-grazing disturbance interaction occurs when areas that have been recently burned are followed by intense grazing due to the high quality of forage regrowth. This interaction has been managed in tallgrass prairies in a homogenous method using uniform burning and grazing and a heterogeneous method using patch-burn-grazing (PBG). Vegetation heterogeneity at a large scale has been studied in PBG and uniformly managed ...


Towards The Development Of A Sustainable Soya Bean-Based Feedstock For Aquaculture, Hyunwoo Park, Steven Weier, Fareha Razvi, Pamela A. Peña, Neil A. Sims, Jennica Lowell, Cory Hungate, Karma Kissinger, Gavin Key, Paul Fraser, Jonathan Napier, Edgar B. Cahoon, Thomas Clemente 2017 University of Nebraska-Lincoln

Towards The Development Of A Sustainable Soya Bean-Based Feedstock For Aquaculture, Hyunwoo Park, Steven Weier, Fareha Razvi, Pamela A. Peña, Neil A. Sims, Jennica Lowell, Cory Hungate, Karma Kissinger, Gavin Key, Paul Fraser, Jonathan Napier, Edgar B. Cahoon, Thomas Clemente

Faculty Publications from the Center for Plant Science Innovation

Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean-based protein and a high Omega-3 fatty acid soya bean oil, enriched with alpha-linolenic and ...


Invasive Species Control And Perennial Plant Establishment In Antelope Pasture, Pocatello, Blm, Kari E. Veblen 2017 Utah State University

Invasive Species Control And Perennial Plant Establishment In Antelope Pasture, Pocatello, Blm, Kari E. Veblen

Funded Research and Data

No abstract provided.


Corn Residue Use By Livestock In The United States, Marty R. Schmer, Rachael M. Brown, Virginia L. Jin, Robert B. Mitchell, Daren D. Redfearn 2017 USDA-ARS

Corn Residue Use By Livestock In The United States, Marty R. Schmer, Rachael M. Brown, Virginia L. Jin, Robert B. Mitchell, Daren D. Redfearn

Agronomy & Horticulture -- Faculty Publications

Corn (Zea mays L.) residue grazing or harvest provides a simple and economical practice to integrate crops and livestock, but limited information is available on how widespread corn residue utilization is practiced by US producers. In 2010, the USDA Economic Research Service surveyed producers from 19 states on corn grain and residue management practices. Total corn residue grazed or harvested was 4.87 million ha. Approximately 4.06 million ha was grazed by 11.7 million livestock (primarily cattle) in 2010. The majority of grazed corn residue occurred in Nebraska (1.91 million ha), Iowa (385,000 ha), South Dakota ...


Supplemental Data, Russell J. Ingram, Foster Levy, Cindy L. Barrett, James T. Donaldson 2017 University of Georgia

Supplemental Data, Russell J. Ingram, Foster Levy, Cindy L. Barrett, James T. Donaldson

ETSU Faculty Works

No abstract provided.


Glucose-Abl1-Tor Signaling Modulates Cell Cycle Tuning To Control Terminal Appressorial Cell Differentiation, Margarita Marroquin-Guzman, Guangchao Sun, Richard Wilson 2017 University of Nebraska-Lincoln

Glucose-Abl1-Tor Signaling Modulates Cell Cycle Tuning To Control Terminal Appressorial Cell Differentiation, Margarita Marroquin-Guzman, Guangchao Sun, Richard Wilson

Papers in Plant Pathology

The conserved target of rapamycin (TOR) pathway integrates growth and development with available nutrients, but how cellular glucose controls TOR function and signaling is poorly understood. Here, we provide functional evidence from the devastating rice blast fungus Magnaporthe oryzae that glucose can mediate TOR activity via the product of a novel carbon- responsive gene, ABL1, in order to tune cell cycle progression during infection-related development. Under nutrient-free conditions, wild type (WT) M. oryzae strains form terminal plant-infecting cells (appressoria) at the tips of germ tubes emerging from three-celled spores (conidia). WT appressorial development is accompanied by one round of mitosis ...


Expression Of Apoplast-Targeted Plant Defensin Mtdef4.2 Confers Resistance To Leaf Rust Pathogen Puccinia Triticina But Does Not Affect Mycorrhizal Symbiosis In Transgenic Wheat, Jagdeep Kaur, John Fellers, Alok Adholeya, Siva L.S. Velivelli, Kaoutar El-Mounadi, Natalya Nersesian, Thomas E. Clemente, Dilip Shah 2017 Donald Danforth Plant Science Center

Expression Of Apoplast-Targeted Plant Defensin Mtdef4.2 Confers Resistance To Leaf Rust Pathogen Puccinia Triticina But Does Not Affect Mycorrhizal Symbiosis In Transgenic Wheat, Jagdeep Kaur, John Fellers, Alok Adholeya, Siva L.S. Velivelli, Kaoutar El-Mounadi, Natalya Nersesian, Thomas E. Clemente, Dilip Shah

Faculty Publications from the Center for Plant Science Innovation

Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control leaf rust, but genetic resistance is ephemeral and breaks down with the emergence of new virulent Pt races. There is a need to develop alternative measures for control of leaf rust in wheat. Development of transgenic wheat expressing an antifungal defensin offers a promising approach to complement the endogenous resistance genes within the wheat ...


The Rna Polymerase-Associated Factor 1 Complex Is Required For Plant Touch Responses, Gregory S. Jensen, Kateryna Fal, Olivier Hamant, Elizabeth S. Haswell 2017 Washington University in St Louis

The Rna Polymerase-Associated Factor 1 Complex Is Required For Plant Touch Responses, Gregory S. Jensen, Kateryna Fal, Olivier Hamant, Elizabeth S. Haswell

Biology Faculty Publications & Presentations

Thigmomorphogenesis is a stereotypical developmental alteration in the plant body plan that can be induced by repeatedly touching plant organs. To unravel how plants sense and record multiple touch stimuli we performed a novel forward genetic screen based on the development of a shorter stem in response to repetitive touch. The touch insensitive (ths1) mutant identified in this screen is defective in some aspects of shoot and root thigmomorphogenesis. The ths1 mutant is an intermediate loss-of-function allele of VERNALIZATION INDEPENDENCE 3 (VIP3), a previously characterized gene whose product is part of the RNA polymerase II-associated factor 1 (Paf1) complex. The ...


Can Functional Traits Predict Plant Community Response To Global Change?, Sarah Kimball, Jennifer L. Funk, Marko J. Spasojevic, Katharine N. Suding, Scot Parker, Michael K. Goulden 2016 University of California - Irvine

Can Functional Traits Predict Plant Community Response To Global Change?, Sarah Kimball, Jennifer L. Funk, Marko J. Spasojevic, Katharine N. Suding, Scot Parker, Michael K. Goulden

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

One primary goal at the intersection of community ecology and global change biology is to identify functional traits that are useful for predicting plant community response to global change. We used observations of community composition from a long-term field experiment in two adjacent plant communities (grassland and coastal sage shrub) to investigate how nine key plant functional traits were related to altered water and nitrogen availability following fire. We asked whether the functional responses of species found in more than one community type were context dependent and whether community-weighted mean and functional diversity were significantly altered by water and nitrogen ...


Assessing Competition Between Invasive And Native Plant Species At Ascot Hills Park, Jacquelyn Galvez 2016 Loyola Marymount University

Assessing Competition Between Invasive And Native Plant Species At Ascot Hills Park, Jacquelyn Galvez

Research & Exhibition

No abstract provided.


Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal 2016 University of Louisville

Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal

Electronic Theses and Dissertations

Unusual monoenoic fatty acids (UMFA’s) and specialized metabolites called anacardic acids (AnAc) are produced in glandular trichomes of Pelargonium ´ hortorum (geranium). The UMFA’s, 16:1∆11 and 18:1∆13 are precursors for the synthesis of unsaturated AnAc 22:1n5and 24:1n5 that contribute to pest resistance in geraniums. UMFAs and their derived AnAc metabolites not only provide a useful biological marker that differentiates the biosynthetic pathway for unusual mononenes from the common fatty acids (i.e. stearic, palmitic, oleic, linoleic and linolenic) but also have industrial, medical and agricultural applications. Fatty acid biosynthesis enzymes like acyl carrier ...


American Chestnut Sem Portfolio, Dakota Matthews 2016 SUNY College of Environmental Science and Forestry

American Chestnut Sem Portfolio, Dakota Matthews

N.C. Brown Center for Ultrastructure Studies

Scanning electron microscopy micrographs of transgenic american chestnut pollen and anthers. Chinkapin anthers and pollen imaged also.


Phytochrome B Integrates Light And Temperature Signals In Arabidopsis, Martina Legris, Cornelia Klose, E Sethe Burgie, Cecilia Costigliolo Rojas Rojas, Maximiliano Neme, Andreas Hiltbrunner, Philip A. Wigge, Eberhard Schäfer, Richard D. Vierstra, Jorge J. Casal 2016 Washington University in St Louis

Phytochrome B Integrates Light And Temperature Signals In Arabidopsis, Martina Legris, Cornelia Klose, E Sethe Burgie, Cecilia Costigliolo Rojas Rojas, Maximiliano Neme, Andreas Hiltbrunner, Philip A. Wigge, Eberhard Schäfer, Richard D. Vierstra, Jorge J. Casal

Biology Faculty Publications & Presentations

Ambient temperature regulates many aspects of plant growth and development, but its sensors are unknown. Here, we demonstrate that the phytochrome B (phyB) photoreceptor participates in temperature perception through its temperature-dependent reversion from the active Pfr state to the inactive Pr state. Increased rates of thermal reversion upon exposing Arabidopsis seedlings to warm environments reduce both the abundance of the biologically active Pfr-Pfr dimer pool of phyB and the size of the associated nuclear bodies, even in daylight. Mathematical analysis of stem growth for seedlings expressing wild-type phyB or thermally stable variants under various combinations of light and temperature revealed ...


The Archaeopalynology Of Crystal River Site (8ci1), Citrus County, Florida, Kendal Jackson 2016 University of South Florida

The Archaeopalynology Of Crystal River Site (8ci1), Citrus County, Florida, Kendal Jackson

Graduate Theses and Dissertations

The Woodland-period (ca. 1000 B.C. to A.D. 1050) fisher-hunter-gatherers of the Crystal River drainage on Florida’s Big Bend Coast are well known among southeastern archaeologists for their elaborate shell mound architecture, maritime lifeway, and exotic exchange goods. Recent archaeological investigations at the Crystal River site have employed high-resolution topographic mapping, geophysical surveys, trench excavations, and coring to model the temporality of mound construction and occupation at the site; this work has set the stage for subsequent research focusing on community structure, resource extraction, and human-ecosystem dynamics. However, like many central and north peninsular Gulf Coast sites, our ...


Studying The Effects Of Serpentine Soil On Adapted And Non-Adapted Species Using Arduino Technology, Kiana Saniee, Edward Himelblau, Brian Paavo 2016 California Polytechnic State University - San Luis Obispo

Studying The Effects Of Serpentine Soil On Adapted And Non-Adapted Species Using Arduino Technology, Kiana Saniee, Edward Himelblau, Brian Paavo

STAR (STEM Teacher and Researcher) Presentations

Abstract: Serpentine soils are formed from ultramafic rocks and are represent an extreme environment for plants. Serpentine soils are unique in that they carry high concentrations of heavy metals, are nutrient deficient, particularly in calcium, and have poor water retention capabilities. Although these soils constitute harsh conditions for plant growth, there are a number of species that are adapted and even endemic to serpentine soil. Water retention by commercial potting mix was compared with serpentine soil. Also, serpentine adapted and non-adapted species were grown in both soil treatments and physiological data were collected. We used the Arduino electronic platform to ...


Orm Expression Alters Sphingolipid Homeostasis And Differentially Affects Ceramide Synthase Activity, Athen N. Kimberlin, Gongshe Han, Kyle D. Luttgeharm, Ming Chen, Rebecca E. Cahoon, Julie M. Stone, Jonathan E. Markham, Teresa M. Dunn, Edgar B. Cahoon 2016 University of Nebraska - Lincoln

Orm Expression Alters Sphingolipid Homeostasis And Differentially Affects Ceramide Synthase Activity, Athen N. Kimberlin, Gongshe Han, Kyle D. Luttgeharm, Ming Chen, Rebecca E. Cahoon, Julie M. Stone, Jonathan E. Markham, Teresa M. Dunn, Edgar B. Cahoon

Faculty Publications from the Center for Plant Science Innovation

Sphingolipid synthesis is tightly regulated in eukaryotes. This regulation in plants ensures sufficient sphingolipids to support growth while limiting the accumulation of sphingolipid metabolites that induce programmed cell death. Serine palmitoyltransferase (SPT) catalyzes the first step in sphingolipid biosynthesis and is considered the primary sphingolipid homeostatic regulatory point. In this report, Arabidopsis (Arabidopsis thaliana) putative SPT regulatory proteins, orosomucoidlike proteins AtORM1 and AtORM2, were found to interact physically with Arabidopsis SPT and to suppress SPT activity when coexpressed with Arabidopsis SPT subunits long-chain base1 (LCB1) and LCB2 and the small subunit of SPT in a yeast (Saccharomyces cerevisiae) SPT-deficient mutant ...


Trends In Nectar Concentration And Hummingbird Visitation: Investigating Different Variables In Three Flowers Of The Ecuadorian Cloud Forest: Guzmania Jaramilloi, Gasteranthus Quitensis, And Besleria Solanoides, Sophie Wolbert 2016 SIT Study Abroad

Trends In Nectar Concentration And Hummingbird Visitation: Investigating Different Variables In Three Flowers Of The Ecuadorian Cloud Forest: Guzmania Jaramilloi, Gasteranthus Quitensis, And Besleria Solanoides, Sophie Wolbert

Independent Study Project (ISP) Collection

Many variables affect hummingbird visitation to flowers, including nectar concentration, time of day, corolla length and other aspects of flower morphology, and elevation. Nectar concentration was measured from flowers of three Ecuadorian subtropical montane species: Guzmania jaramilloi, Gasteranthus quitensis, and Besleria solanoides. Concentration values were compared across varying times of day, corolla lengths, and elevations to determine the effects of these variables on flower nectar concentration. Camera traps were also used to observe hummingbird visitation to the above three flower species. After running regression and ANOVA analyses, nectar concentration was found to be positively correlated with time of day, negatively ...


Arthur Rice Kruckeberg: Geobotanist Extraordinaire (1920–2016), Nishanta Rajakaruna 2016 California Polytechnic State University, San Luis Obispo

Arthur Rice Kruckeberg: Geobotanist Extraordinaire (1920–2016), Nishanta Rajakaruna

Nishanta Rajakaruna

No abstract provided.


Bacterial Leaf Streak Of Corn Confirmed In Nebraska, Other Corn Belt States August 26, 2016, Tamra Jackson-Ziems, Kevin A. Korus, Tony Adesemoye, Julie Van Meter 2016 University of Nebraska-Lincoln

Bacterial Leaf Streak Of Corn Confirmed In Nebraska, Other Corn Belt States August 26, 2016, Tamra Jackson-Ziems, Kevin A. Korus, Tony Adesemoye, Julie Van Meter

Papers in Plant Pathology

Bacterial leaf streak disease of corn, caused by Xanthomonas vasicola pv. vasculorum, has now been confirmed in Nebraska, as well as in Colorado, Illinois, Iowa, and Kansas. The disease had not been previously identified in the U.S., but had been reported on corn in South Africa. Surveys are currently underway across the Corn Belt to identify the disease distribution. Initial observations and survey results suggest that it may be widely distributed throughout the Corn Belt. Unusual symptoms were first reported on corn samples received by the University of Nebraska Plant and Pest Diagnostic Clinic. Over the last two years ...


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