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Structural Basis For Light Control Of Cell Development Revealed By Crystal Structures Of A Myxobacterial Phytochrome, Nicole C. Woitowich, Andrei S. Halavaty, Patricia Waltz, Christopher Kupitz, Joseph Valera, Gregory Tracy, Kevin D. Gallagher, Elin Claesson, Takanori Nakane, Suraj Pandey, Garrett Nelson, Rie Tanaka, Eriko Nango, Eiichi Mizohata, Shigeki Owada, Kensure Tono, Yasumasa Joti, Angela C. Nugent, Hardik Patel, Ayesha Mapara, James Hopkins, Phu Duong, Dorina Bizhga, Svetlana E. Kovaleva, Rachel St. Peter, Cynthia N. Hernandez, Wesley B. Ozarowski, Shatabdi Roy-Chowdhuri, Jay-How Yang, Petra Edlund, Heikki Takala, Janne Ihalainen, Jennifer Brayshaw, Tyler Norwood, Ishwor Poudyal, Petra Fromme, John C.H. Spence, Keith Moffat, Sebastian Westenhoff, Marius Schmidt, Emina A. Stojkovic 2018 Northeastern Illinois University

Structural Basis For Light Control Of Cell Development Revealed By Crystal Structures Of A Myxobacterial Phytochrome, Nicole C. Woitowich, Andrei S. Halavaty, Patricia Waltz, Christopher Kupitz, Joseph Valera, Gregory Tracy, Kevin D. Gallagher, Elin Claesson, Takanori Nakane, Suraj Pandey, Garrett Nelson, Rie Tanaka, Eriko Nango, Eiichi Mizohata, Shigeki Owada, Kensure Tono, Yasumasa Joti, Angela C. Nugent, Hardik Patel, Ayesha Mapara, James Hopkins, Phu Duong, Dorina Bizhga, Svetlana E. Kovaleva, Rachel St. Peter, Cynthia N. Hernandez, Wesley B. Ozarowski, Shatabdi Roy-Chowdhuri, Jay-How Yang, Petra Edlund, Heikki Takala, Janne Ihalainen, Jennifer Brayshaw, Tyler Norwood, Ishwor Poudyal, Petra Fromme, John C.H. Spence, Keith Moffat, Sebastian Westenhoff, Marius Schmidt, Emina A. Stojkovic

Biology Faculty Publications

Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs in photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr and Pfr with distinct absorption spectra in the red and far-red. They have recently been engineered as enzymatic photoswitches for fluorescent-marker applications in non-invasive tissue imaging of mammals. This article presents cryo- and room-temperature crystal structures of the unusual phytochrome from the non-photosynthetic myxobacterium Stigmatella aurantiaca (SaBphP1) and reveals its role in the fruitingbody formation of this photomorphogenic bacterium. SaBphP1 lacks a conserved histidine (His) in the chromophore-binding ...


Effects Of Foliar Redox Status On Leaf Vascular Organization Suggest Avenues For Cooptimization Of Photosynthesis And Heat Tolerance, Jared J. Stewart, Christopher R. Baker, Carlie S. Sharpes, Shannon Toy Wong-Michalak, Stephanie K. Polutchko, William W. Adams III, Barbara Demmig-Adams 2018 University of Colorado Boulder

Effects Of Foliar Redox Status On Leaf Vascular Organization Suggest Avenues For Cooptimization Of Photosynthesis And Heat Tolerance, Jared J. Stewart, Christopher R. Baker, Carlie S. Sharpes, Shannon Toy Wong-Michalak, Stephanie K. Polutchko, William W. Adams Iii, Barbara Demmig-Adams

University Libraries Open Access Fund Supported Publications

The interaction of heat stress with internal signaling networks was investigated through Arabidopsis thaliana mutants that were deficient in either tocopherols (vte1 mutant) or non-photochemical fluorescence quenching (NPQ; npq1, npq4, and npq1 npq4 mutants). Leaves of both vte1 and npq1 npq4 mutants that developed at a high temperature exhibited a significantly different leaf vascular organization compared to wild-type Col-0. Both mutants had significantly smaller water conduits (tracheary elements) of the xylem, but the total apparent foliar water-transport capacity and intrinsic photosynthetic capacity were similarly high in mutants and wild-type Col-0. This was accomplished through a combination of more numerous (albeit ...


Yardwork: A Biography Of An Urban Place By Daniel Coleman, Vivian M. Hansen 2018 University of Calgary

Yardwork: A Biography Of An Urban Place By Daniel Coleman, Vivian M. Hansen

The Goose

Review of Daniel Coleman's Yardwork: A Biography of an Urban Place.


Genetic Diversity In An Invasive Clonal Plant? A Historical And Contemporary Perspective, Elliot D. Weidow 2018 University of New Orleans

Genetic Diversity In An Invasive Clonal Plant? A Historical And Contemporary Perspective, Elliot D. Weidow

University of New Orleans Theses and Dissertations

Introduced populations of Eichhornia crassipes (Pontederiaceae) possess extremely low levels of genetic diversity due to severe bottleneck events and clonal reproduction. While populations elsewhere have been well studied, North American populations of E. crassipes remain understudied. We used Amplified Fragment Length Polymorphism markers to assess genetic diversity and population structure in North American E. crassipes populations. Patterns of diversity over the past fifty years were analyzed using herbarium specimens. Furthermore, we sampled populations across the Gulf Coast of the United States throughout a year to determine contemporary genetic diversity and assess potential seasonal effects. Genetic diversity was found to be ...


Identification And Characterization Of Salinity Tolerance Genes By Activation Tagging In Arabidopsis, Jawaher Alkahtani 2018 University of Arkansas, Fayetteville

Identification And Characterization Of Salinity Tolerance Genes By Activation Tagging In Arabidopsis, Jawaher Alkahtani

Theses and Dissertations

Salinity often affects irrigated areas in arid and semi-arid regions of the world. The existence and accumulation of soluble salts in the soil layers limit the growth of crops essential for our food. Salt stress dramatically affects plant growth, plant development, as well as crop yield. Arabidopsis thaliana is the plant model that provides a comprehensive knowledge of plant development, genetics and physiology, and response to abiotic stresses such as salinity. The redundancy of genes due to duplication, even in the simple model genome of Arabidopsis, limits the value of knockout (KO) mutagenesis to provide complete information on gene function ...


Association Mapping And Genomic Selection For Yield And Agronomic Traits In Soft Winter Wheat, Dennis Bulusan Lozada 2018 University of Arkansas, Fayetteville

Association Mapping And Genomic Selection For Yield And Agronomic Traits In Soft Winter Wheat, Dennis Bulusan Lozada

Theses and Dissertations

Tools such as genome-wide association study (GWAS) and genomic selection (GS) have expedited the development of crops with improved genetic potential. While GWAS aims to identify significant markers associated with a trait of interest, the goal of GS is to utilize all marker effects to predict the performance of new breeding lines prior to testing. A GWAS for grain yield (GY), yield components, and agronomic traits was conducted using a diverse panel of 239 soft winter wheat (SWW) lines evaluated in eight site-years in Arkansas and Oklahoma. Broad sense heritability of GY (H2=0.48) was moderate compared to other ...


Floristic Survey Of Crawford And Cherokee Counties In Southeast Kansas: An Evaluation Of Change Over Five Decades, Samantha Pryer 2018 Pittsburg State University

Floristic Survey Of Crawford And Cherokee Counties In Southeast Kansas: An Evaluation Of Change Over Five Decades, Samantha Pryer

Electronic Thesis Collection

This project had two main objectives. The first objective was to document comprehensively the vascular flora of Crawford and Cherokee counties in extreme southeast Kansas. Each county had seen limited collecting in recent decades, though a comprehensive survey had never been done for Cherokee County. PSU student E.S. Gibson summarized the flora for Crawford County in 1963. Fieldwork for the present study occurred mostly in 2014 and 2015. Based on 6500+ newly collected specimens, vouchers from Gibson’s study, and taxa documented by Biota of North America (BONAP) (Kartesz 2017), 1440 taxa are reported, which includes 33 state and ...


Leaf Micromorphology Aids Taxonomic Delineation Within The Hypervariable Genus Metrosideros (Myrtaceae) On O'Ahu, Gary L. Sur, Richard Keating, Elizabeth Stacy 2018 University of Hawaii, Hilo

Leaf Micromorphology Aids Taxonomic Delineation Within The Hypervariable Genus Metrosideros (Myrtaceae) On O'Ahu, Gary L. Sur, Richard Keating, Elizabeth Stacy

Life Sciences Faculty Publications

Variation in leaf micromorphology can aid delimitation of taxonomically difficult groups. The woody genus Metrosideros (Myrtaceae) dominates Hawai'i's native forests and comprises striking, predominantly infraspecific, macromorphological variation, including many forms unrecognized in current taxonomic treatments. On taxonomically rich O'ahu, 10 taxa (varieties or unnamed morphotypes of M. polymorpha and other species) occur in a predictable sequence with overlapping ranges from low to high elevation along the numerous leeward ridges of the Ko'olau Range. We used scanning electron and light microscopy to examine mature, nonsenescing sun leaves from each of these 10 taxa. Parametric and nonparametric tests ...


The Effect Of Kalanchoe Pinnata On The Cell Migration Of Astrocytomas, Jorge Rosa 2018 University High School

The Effect Of Kalanchoe Pinnata On The Cell Migration Of Astrocytomas, Jorge Rosa

The International Student Science Fair 2018

Astrocytomas, from cell line 1321N1,are cancerous astrocytes. These are a type of glial cell in the brain which is responsible for maintaining synaptic contact and the signaling capacities among neurons. They also represent a fundamental element in controlling blood flow and defining brain micro structure. Due to the fact that this variant is cancerous, a condition commonly described as an abnormal cell cycle and uncontrolled reproduction of cells, they have the ability to metastasize.

In the metastatic process of cancer cells the initial phase is know as tumor invasion. This stage consists mainly of the cells migrating away from ...


Lipid Transport Required To Make Lipids Of Photosynthetic Membranes, Evan LaBrant, Allison C. Barnes, Rebecca Roston 2018 University of Nebraska–Lincoln

Lipid Transport Required To Make Lipids Of Photosynthetic Membranes, Evan Labrant, Allison C. Barnes, Rebecca Roston

Biochemistry -- Faculty Publications

Photosynthetic membranes provide much of the usable energy for life on earth. To produce photosynthetic membrane lipids, multiple transport steps are required, including fatty acid export from the chloroplast stroma to the endoplasmic reticulum, and lipid transport from the endoplasmic reticulum to the chloroplast envelope membranes. Transport of hydrophobic molecules through aqueous space is energetically unfavorable and must be catalyzed by dedicated enzymes, frequently on specialized membrane structures. Here, we review photosynthetic membrane lipid transport to the chloroplast in the context of photosynthetic membrane lipid synthesis. We independently consider the identity of transported lipids, the proteinaceous transport components, and membrane ...


The Effect Of Kalanchoe Pinnata In Astrocitome Metastasis, Jorge Rosa 2018 University High School

The Effect Of Kalanchoe Pinnata In Astrocitome Metastasis, Jorge Rosa

The International Student Science Fair 2018

Astrocytomas, from cell line 1321N1,are cancerous astrocytes. These are a type of glial cell in the brain which is responsible for maintaining synaptic contact and the signaling capacities among neurons. They also represent a fundamental element in controlling blood flow and defining brain micro structure. Due to the fact that this variant is cancerous, a condition commonly described as an abnormal cell cycle and uncontrolled reproduction of cells, they have the ability to metastasize.

In the metastatic process of cancer cells the initial phase is know as tumor invasion. This stage consists mainly of the cells migrating away from ...


Field Pea Response To Seeding Rate, Depth, And Inoculant In West-Central Nebraska, Strahinja V. Stepanovic, Charles Burr, Julie A. Peterson, Daran Rudnick, Cody F Creech, Rodrigo Werle 2018 University of Nebraska-Lincoln

Field Pea Response To Seeding Rate, Depth, And Inoculant In West-Central Nebraska, Strahinja V. Stepanovic, Charles Burr, Julie A. Peterson, Daran Rudnick, Cody F Creech, Rodrigo Werle

West Central Research and Extension Center, North Platte

Increased market demand and larger adoption of field pea (Pisum sativum L.) in semiarid west-central Nebraska has provided opportunities to replace summer fallow and diversify crop rotations. As a relatively new crop, its response to different seeding practices has not been evaluated in this eco-region. Field pea grain yield response to seeding depth (25, 50, and 75 mm), inoculation with Rhizobium leguminosarum bv. viciae (yes and no rhizobia inoculant), and seeding rates (35, 50, 65, 75, 90, 105, and 120 plants m–2) was investigated in 2015 and 2016 at five sites in Perkins County, NE. There were no differences ...


Nonpolar Residues In The Presumptive Pore‐Lining Helix Of Mechanosensitive Channel Msl10 Influence Channel Behavior And Establish A Nonconducting Function, Grigory Maksaev, Jennette K. Shoots, Simran Ohri, Elizabeth S. Haswell 2018 Washington University in St Louis

Nonpolar Residues In The Presumptive Pore‐Lining Helix Of Mechanosensitive Channel Msl10 Influence Channel Behavior And Establish A Nonconducting Function, Grigory Maksaev, Jennette K. Shoots, Simran Ohri, Elizabeth S. Haswell

Biology Faculty Publications & Presentations

Mechanosensitive (MS) ion channels provide a universal mechanism for sensing and responding to increased membrane tension. MscS‐like (MSL) 10 is a relatively well‐studied MS ion channel from Arabidopsis thaliana that is implicated in cell death signaling. The relationship between the amino acid sequence of MSL10 and its conductance, gating tension, and opening and closing kinetics remains unstudied. Here, we identify several nonpolar residues in the presumptive pore‐lining transmembrane helix of MSL10 (TM6) that contribute to these basic channel properties. F553 and I554 are essential for wild type channel conductance and the stability of the open state. G556 ...


Arabidopsis Thaliana Ei-5: Minor Vein Architecture Adjustment Compensates For Low Vein Density In Support Of Photosynthesis, Jared J. Stewart, Stephanie K. Polutchko, Barbara Demmig-Adams, William W. Adams III 2018 University of Colorado Boulder

Arabidopsis Thaliana Ei-5: Minor Vein Architecture Adjustment Compensates For Low Vein Density In Support Of Photosynthesis, Jared J. Stewart, Stephanie K. Polutchko, Barbara Demmig-Adams, William W. Adams Iii

University Libraries Open Access Fund Supported Publications

An Arabidopsis thaliana accession with naturally low vein density, Eifel-5 (Ei-5), was compared to Columbia-0 (Col-0) with respect to rosette growth, foliar vein architecture, photosynthesis, and transpiration. In addition to having to a lower vein density, Ei-5 grew more slowly, with significantly lower rates of rosette expansion, but had similar capacities for photosynthetic oxygen evolution on a leaf area basis compared to Col-0. The individual foliar minor veins were larger in Ei-5, with a greater number of vascular cells per vein, compared to Col-0. This compensation for low vein density resulted in similar values for the product of vein density ...


Biological Soil Crusts In A Northeastern Pine Barren: Composition And Ecological Effects, Jessica Gilbert 2018 Union College - Schenectady, NY

Biological Soil Crusts In A Northeastern Pine Barren: Composition And Ecological Effects, Jessica Gilbert

Honors Theses

Biological soil crusts (BSCs), otherwise known as cryptogamic soil crusts, biocrusts, or cyanobacterial crusts, are soil aggregations hosting diverse biotic communities. They are composed of cyanobacteria and algae, and generally have a covering of moss and/or lichen. BSCs are typically found in arid to semi-arid regions throughout the world, and are integral soil stabilizers, moisture retainers, and nitrogen fixers in these communities. Along with these factors, BSCs are able to impact germination and establishment of plants, either as an accompanying influence, or direct result of those listed above. BSCs have yet to be formally described in the inland northeastern ...


Generation Of Nonlinear-Differential-Equations System From A Model Of Boolean Relationships In Arabidopsis Salt Stress Network, Renee Dale 2018 Louisiana State University and Agricultural and Mechanical College

Generation Of Nonlinear-Differential-Equations System From A Model Of Boolean Relationships In Arabidopsis Salt Stress Network, Renee Dale

Biology and Medicine Through Mathematics Conference

No abstract provided.


Factors Affecting The Regrowth Of Ilex Glabra In A Routinely Burned Longleaf Forest, Jaybus Price 2018 The University of Southern Mississippi

Factors Affecting The Regrowth Of Ilex Glabra In A Routinely Burned Longleaf Forest, Jaybus Price

Master's Theses

This study examines the effects of historical management by use of prescribed fire on Ilex glabra stems/m2 and factors affecting the regrowth of I. glabra after a prescribed burn to gain beneficial knowledge for management purposes. Environmental factors and morphological parameters of I. glabra were sampled before and after a prescribed burn of the Longleaf Trace Nature Preserve in September 2016. The study site is located in Lamar County, Mississippi, just west of Hattiesburg, MS. Stem densities of I. glabra were collected once before the prescribed burn and twice after the prescribed burn at 2 month and 9 ...


Investigation Of The Role Of Gene Clusters In Terpene Biosynthesis In Sorghum Bicolor, Rebecca Hay 2018 University of Missouri, St. Louis

Investigation Of The Role Of Gene Clusters In Terpene Biosynthesis In Sorghum Bicolor, Rebecca Hay

Theses

The staple crop Sorghum bicolor shows potential as a source of secondary metabolite-based biofuels due to its diverse phenotype and chemical profile. S. bicolor produces a variety of high-energy metabolites, including terpenes which are a potential renewable source of fuel additives. Information on the biosynthetic and genetic pathways by which S. bicolor terpenes are produced is limited and these pathways must be better understood before they can be engineered for human applications. Recent work on plant biosynthetic pathways has shown that terpenes can be modified by the products of clustered genes. Identification of biosynthetic gene clusters may accelerate the elucidation ...


The Role Of Ferric Reduction Oxidases In Plant Anti-Herbivore Defense., Virginia C. Nunamaker 2018 University of Louisville

The Role Of Ferric Reduction Oxidases In Plant Anti-Herbivore Defense., Virginia C. Nunamaker

College of Arts & Sciences Senior Honors Theses

Iron is an essential element required for plants to carry out metabolic functions such as photosynthesis, heme biosynthesis, and chlorophyll biosynthesis. Within Arabidopsis thaliana, eight ferric reduction oxidase (FRO) genes function in iron uptake and homeostasis with tissue specific expression. However, little else is known regarding the biological role of FROs. Recent studies identify the FRO gene family as particularly responsive to the green leaf plant derived volatile (GLV) cis-3-hexenyl acetate (z3HAC). Since z3HAC acts as a wound signal and cues unaffected parts of the plant to prime defenses prior to herbivory, an increase in FRO activity in response to ...


Non-Target-Site Based Tolerance To Herbicides In Amaranthus Palmeri, Reiofeli Algodon Salas-Perez 2018 University of Arkansas, Fayetteville

Non-Target-Site Based Tolerance To Herbicides In Amaranthus Palmeri, Reiofeli Algodon Salas-Perez

Theses and Dissertations

Palmer amaranth, one of the most aggressive and damaging broadleaf weeds in the USA, has evolved resistance to multiple herbicide modes of action. The overall objective of this research was to elucidate the mechanisms by which Palmer amaranth adapt to herbicide selection stress. This research aimed (1) to evaluate the efficacy of fomesafen, glufosinate, glyphosate and trifloxysulfuron to Amaranthus populations; (2) identify candidate genes for endowing tolerance to glufosinate; (3) investigate the involvement of non-target-site resistance (NTSR) mechanism in an ALS-resistant population; and (4) to examine the molecular basis of resistance to PPO inhibitors in Palmer amaranth populations from Arkansas ...


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