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Increasing Production Of Therapeutic Mabs In Cho Cells Through Genetic Engineering, Charles Barentine 2022 Utah State University

Increasing Production Of Therapeutic Mabs In Cho Cells Through Genetic Engineering, Charles Barentine

All Graduate Theses and Dissertations

Between 2014 and 2018, the global market for therapeutic monoclonal antibodies (mAbs) rose from $60 billion to $115.2 billion with a projected value of $300 billion by 2025. These molecules are used to effectively treat some of the most challenging illnesses from auto-immune diseases to cancer. While mAbs are highly valuable with potent applications, their production at scale remains an outstanding challenge. These molecules are largely produced in Chinese Hamster Ovary (CHO) cells that require highly specific conditions to produce a useful product.

Genetic engineering presents one solution to overcome productivity limits. With the advent of CRISPR (clustered regularly ...


Low Resource Assay For Tracking Sars-Cov-2 In Wastewater, Julissa van Renselaar 2022 Utah State University

Low Resource Assay For Tracking Sars-Cov-2 In Wastewater, Julissa Van Renselaar

All Graduate Theses and Dissertations

Wastewater based epidemiology (WBE) allows for the tracking of nucleic acid of SARS-CoV-2 in wastewater which gives the opportunity for the public and government officials to be informed about the infectivity of the virus in a community. Advances have been made in WBE that have allowed for higher performance, lower resource use, and faster turnaround time. An adapted concentration method of spin column direct extraction has yielded a proxy virus recovery of 83%, consumable cost of $2.01 per sample, and a turnaround time of 0.33 hour/sample. Other concentration methods have lower proxy virus recoveries, comparable cost, and ...


Predicting Insulin Pump Therapy Settings, Riccardo L. Ferraro, David Grijalva, Alex Trahan 2022 Southern Methodist University & Tandem Diabetes Care, Inc

Predicting Insulin Pump Therapy Settings, Riccardo L. Ferraro, David Grijalva, Alex Trahan

SMU Data Science Review

Millions of people live with diabetes worldwide [7]. To mitigate some of the many symptoms associated with diabetes, an estimated 350,000 people in the United States rely on insulin pumps [17]. For many of these people, how effectively their insulin pump performs is the difference between sleeping through the night and a life threatening emergency treatment at a hospital. Three programmed insulin pump therapy settings governing effective insulin pump function are: Basal Rate (BR), Insulin Sensitivity Factor (ISF), and Carbohydrate Ratio (ICR). For many people using insulin pumps, these therapy settings are often not correct, given their physiological needs ...


Effects Of Cell-Cell Signaling On Mesenchymal Stem Cell Mechanosensing And Adaptation To Dynamic Material, Matthew E. Lowe 2022 Rowan University

Effects Of Cell-Cell Signaling On Mesenchymal Stem Cell Mechanosensing And Adaptation To Dynamic Material, Matthew E. Lowe

Theses and Dissertations

The goal of this research is to identify the role of engineered cell-cell signals on how cells sense material properties. Mesenchymal stem cells (MSCs) are adult cells whose behavior is regulated by matrix mechanosensing, which is characterized by stiffness-dependent changes in cell shape and the nuclear localization of mechano-transducer proteins including YAP (Yes-associated Protein). MSC area and nuclear YAP translocation increase with increasing stiffness, and although low levels of N-cadherin-based cell-cell signaling reduce this effect, two fundamental questions remain: (1) do engineered cell-cell signals at higher concentrations further reduce matrix mechanosensing, and (2) does N-cadherin signaling affect MSC adaptation to ...


Modelling And Evaluation Of Piezoelectric Actuators For Wearable Neck Rehabilitation Devices, Shaemus D. Tracey 2022 The University of Western Ontario

Modelling And Evaluation Of Piezoelectric Actuators For Wearable Neck Rehabilitation Devices, Shaemus D. Tracey

Electronic Thesis and Dissertation Repository

Neck pain is the most common neck musculoskeletal disorder, and the fourth leading cause of healthy years lost due to disability in the world. Due to the need of hands-on physical therapy and Canada’s aging population, access to treatment will become highly constrained. Wearable devices that allow at-home rehabilitation address this future limitation. However, few have emerged from the laboratory setting because they are limited by the use of conventional actuators. An overlooked type of actuation technology is that of piezoelectric actuators, more specifically, travelling wave ultrasonic motors (TWUM).

In this work, a clear procedure that outlines how the ...


Abbott Cardiac Electrophysiology Wet Lab Project, Melissa Kurani, Fiona Lynn 2022 California Polytechnic State University, San Luis Obispo

Abbott Cardiac Electrophysiology Wet Lab Project, Melissa Kurani, Fiona Lynn

Biomedical Engineering: Graduate Reports and Projects

Cardiac mapping systems provide electrophysiologists with pertinent information about ablation treatment plans for patients who suffer from cardiac arrhythmias. This thesis describes the process of designing a functional wet lab that integrates with Abbott’s EnSite Precision 3D Mapping System, with the purpose of providing Cal Poly students and faculty with an opportunity to have a hands-on learning experience with cardiac mapping. This project encompassed a thorough literature review of cardiology, electrophysiology, and in vitro lab systems, followed by the design, manufacturing, and evaluation of a functional and anatomically representative wet lab. This is a continuation of previous master’s ...


Potassium And Sodium Sensing Isfet Device And Array, Liam Stephen Hayes 2022 California Polytechnic State University, San Luis Obispo

Potassium And Sodium Sensing Isfet Device And Array, Liam Stephen Hayes

Electrical Engineering

The use of Ion-Sensitive Field Effect Transistors (ISFETs) as a means of testing a person’s potassium concentration in real-time has broad applications in the consumer space. An avid runner could use such a device to keep track of their hydration and salt levels. A hospital could use it for patients who require around-the-clock remote monitoring, and a variation of ISFETs are currently being used as continuous glucose monitors for diabetes patients. While ISFETs are not a new development in the field of microelectronics, their use as wearable devices has recently become relevant. The goal of this project is primarily ...


Sensorimotor Content Of Multi-Unit Activity In The Paramedian Lobule Of The Cerebellum, Esma Cetinkaya 2022 New Jersey Institute of Technology

Sensorimotor Content Of Multi-Unit Activity In The Paramedian Lobule Of The Cerebellum, Esma Cetinkaya

Dissertations

Based on Center for Disease Control and Prevention report 2016, around 39.5 million people in the United States suffer from motor disabilities. These disabilities are due to traumatic conditions like traumatic brain injury (TBI), neurological diseases such as amyotrophic lateral sclerosis (ALS), or congenital conditions. One of the approaches for restoring the lost motor function is to extract the volitional information from the central nervous system (CNS) and control a mechanical device that can replace the function of a paralyzed limb through systems called Brain-Computer Interfaces (BCI).

One of the major challenges being faced in BCIs and also in ...


Addressable Microfluidics Platform For Minimally-Invasive Manipulation Of Cells And Chemicals Within Live Cultures, Nguyen Nhat Anh Tong 2022 New Jersey Institute of Technology

Addressable Microfluidics Platform For Minimally-Invasive Manipulation Of Cells And Chemicals Within Live Cultures, Nguyen Nhat Anh Tong

Dissertations

Cell experiments are ubiquitous to the studies of biology, tissue engineering, and drug testing. However, complex cultures are notoriously difficult to analyze nondestructively. Instead, they are typically evaluated using sacrificial means: such as histology sectioning, or by crushing the sample for chemical plate-reader assays. This is inefficient, costly, and results in data discontinuity because each new experiment only provides a single time point. Likewise, delivering new cells or chemicals to custom locations without disturbing an on-going experiment is also difficult. This limits the type of experiments that are feasible. And the inability to deliver nutrients results in cell death in ...


Interactive Software-Based Modeling For Gait Analysis Of Musculoskeletal Structures, Nada Masood Mirza, Adnan Ali, Mohamad Khairi Ishak 2022 School of Electric and Electronic Engineering, Universiti Sains Malaysia, Malaysia, 14300, Nibong Tebal, Penang, Malaysia.

Interactive Software-Based Modeling For Gait Analysis Of Musculoskeletal Structures, Nada Masood Mirza, Adnan Ali, Mohamad Khairi Ishak

Makara Journal of Technology

Software for interactive musculoskeletal modeling applies diverse scientific and technological concepts to stimulate the movement of musculoskeletal figures. Several tools are available for biomechanical analysis in studying motion and capturing the musculoskeletal representation data to facilitate further evaluation for muscle activation. Musculoskeletal software, such as the OpenSim model, animates, and measures in 3D the structural movement of bones, muscles, joints, ligaments, and such structures in the human body. Users apply graphical interfaces to manipulate the movement science for fast and accurate analysis. OpenSim simulation software features a user-friendly interface to allow the proper clinical application in biomechanics and rehabilitation research ...


Analysis And Visualization Of Molecular Docking 2hi4 Protein, innas widiasti 2022 Universitas Indonesia

Analysis And Visualization Of Molecular Docking 2hi4 Protein, Innas Widiasti

Indonesian Journal of Medical Chemistry and Bioinformatics

The crystal structure of the human microsomal complex P450 1A2 with alpha-naphthoflavone, a cytochrome P450 (CYP) enzyme is particularly important, as it is abundant in the human liver and alters a more diverse xenobiotic array than any other group of metabolic enzymes. CYP1A2 is abundantly found in the liver and involved in the metabolism of about 10% of clinically used drugs metabolized by CYP enzymes. The current drug discovery and development mostly uses high-throughput screening (HTS). However, this regular method is time-consuming and costly. To address the issue, an advanced drug discovery and development method namely chemical compound database screening ...


Open-Source And 3-D Printed Autoinjector, Anjutha Selvaraj 2022 Western University

Open-Source And 3-D Printed Autoinjector, Anjutha Selvaraj

Undergraduate Student Research Internships Conference

Autoinjectors have become popular modern injectable medical devices used as drug delivery systems. Due to its ease, capability and reliability compared to other conventional injectable medical devices, the market and manufacturing demand for autoinjector devices are increasing at a staggering rate. Although autoinjectors can offset healthcare treatment costs through self-administered medication, they are expensive for consumers. This paper describes the design and manufacture of a spring-driven and 3-D printed autoinjector using open-source hardware. By making it an open-source resource for everyone, the barrier of advanced research in design and production can be lifted, thus making it easily manufacturable and cost-effective ...


The Role Of Small Gtpases In Regulating Blood-Brain Barrier Mechanotransduction, Brandon Jude DeOre 2022 Rowan University

The Role Of Small Gtpases In Regulating Blood-Brain Barrier Mechanotransduction, Brandon Jude Deore

Theses and Dissertations

Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying the effect of shear on the blood-brain barrier (BBB) have yet to be clarified in cerebral vasculature despite its importance for brain homeostasis.. Neurological symptoms including the formation of microclots, stroke, and other neurological pathologies associated with changes in cerebral blood flow are hallmarks of BBB dysfunction. The in vitro model used in this dissertation is compatible with real-time measurement of barrier function using a transendothelial electrical resistance as well as immunocytochemistry and dextran permeability assays. These experiments reveal that there is a threshold level ...


Multiscale Anisotropy Analysis Of Second-Harmonic Generation Imaging Of Pancreatic Cancer, Joshua D. Hamilton 2022 University of Maine

Multiscale Anisotropy Analysis Of Second-Harmonic Generation Imaging Of Pancreatic Cancer, Joshua D. Hamilton

Electronic Theses and Dissertations

Despite recent advancements in biomedicine, cancer is still the second leading cause of death in the United States. Early detection of cancer is critical to improving patient care, but there are risks of over screening caused by the need for surgical biopsies in many cancers for final diagnostics. Recent advancements in computer aided diagnostics for breast cancer screening has reduced the need for biopsies and resulted in earlier diagnoses which has lowered the mortality rate from breast cancer within the past two decades. Developing new computer aided diagnostic tools that can be applied to a vast majority of cancers would ...


Morphology And Strain In The Subchondral Trabeculae Of Osteoarthritic Humeral Heads, Anna Yang 2022 Western University

Morphology And Strain In The Subchondral Trabeculae Of Osteoarthritic Humeral Heads, Anna Yang

Undergraduate Student Research Internships Conference

This project compares the strains and morphological parameters of subchondral bone in osteoarthritic and non-pathogenic cadaveric humeral heads.


The Development Of A Motion Sensing Device For Use In A Home Setting, Jaspreet K. Kalsi 2022 The University of Western Ontario

The Development Of A Motion Sensing Device For Use In A Home Setting, Jaspreet K. Kalsi

Electronic Thesis and Dissertation Repository

Parkinson's disease (PD) is the second most prevalent neurodegenerative disease, with over 10 million individuals diagnosed with PD world-wide. The most common symptom characterized by PD is tremor. Tremor is an involuntary oscillatory motion that most prominently occurs in upper limb, specifically in the hand and wrist that has a measurable frequency and amplitude. This thesis aims to evaluate the usability and functionality of a tremor sensing device designed to collect quantitative data on individuals with PD. The designed device uses 23 commercially-available inertial measuring units (IMUs) located between 21 joints: distal interphalangeal (DIP) joints, proximal interphalangeal (PIP) joints ...


Bioengineered Scaffolds To Induce Alignment And Promote Axon Regeneration Following Spinal Cord Injury, Kiet Anh Tran 2022 Rowan University

Bioengineered Scaffolds To Induce Alignment And Promote Axon Regeneration Following Spinal Cord Injury, Kiet Anh Tran

Theses and Dissertations

Scaffolds delivered to injured spinal cords to stimulate axon connectivity often act as a bridge to stimulate regeneration at the injured area, but current approaches lack the permissiveness, topology and mechanics to mimic host tissue properties. This dissertation focuses on bioengineering scaffolds through the means of altering topology in injectables and tuning mechanics in 3D-printed constructs as potential therapies for spinal cord injury repair. A self-assembling peptide scaffold, RADA-16I, is used due to its established permissiveness to axon growth and ability to support vascularization. Immunohistochemistry assays verify that vascularized peptide scaffolds promote axon infiltration, attenuate inflammation and reduce astrogliosis. Furthermore ...


Normative Joint Angles And Compensation During Fit-Hansa Test, Jennifer J. Villeneuve 2022 Western University

Normative Joint Angles And Compensation During Fit-Hansa Test, Jennifer J. Villeneuve

Undergraduate Student Research Internships Conference

Imagine a world where no injuries occurred, muscular compensation was able to be detected early on and imbalances resolved before any risks appear. Muscular compensation can cause injuries, especially with repetitive motion or through activities of daily living. The study I conducted focuses on the compensation methods of shoulder abduction and trunk lean during the FIT-HaNSA series. Determining the normative ranges of motion can increase early detection of fatigue for all individuals. This can help make repetitive jobs such as working in a factory much safer for all employees, as they can switch tasks as soon as fatigue is identified ...


Morphology Of The Acromion And Scapular Spine With Special Interest In The Strength & Failure Prediction After Reverse Total Shoulder Replacement, Andrei M. Matusa, Cole Fleet, George S. Athwal, James A. Johnson 2022 Western University

Morphology Of The Acromion And Scapular Spine With Special Interest In The Strength & Failure Prediction After Reverse Total Shoulder Replacement, Andrei M. Matusa, Cole Fleet, George S. Athwal, James A. Johnson

Undergraduate Student Research Internships Conference

Scapular spine fractures following a reverse arthroplasty are a significant clinical concern. This ongoing study looks at the morphology of the acromion and scapular spine with an interest in the strength and failure prediction of the bone. Digital 3D models of the scapular spine were created from cadaver CTs, then bone density and distribution data was obtained. It was found that the cortical bone was most dense just medial of the lateral angle. The cross-sectional area here was also the largest and decreased medially. This has important implications for implant fixation hardware as cortical bone --compared to cancellous bone-- is ...


Tca Fabrication Devices, Brendan R. DiFabio 2022 Western University

Tca Fabrication Devices, Brendan R. Difabio

Undergraduate Student Research Internships Conference

Twisted coil actuators are a recent technology that has different properties from popular actuators used today. Being so new, there is still much to learn about these actuators. This project aims to create devices that can be used to make the fabrication process simple and repeatable.


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