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Molecular, Cellular, and Tissue Engineering Commons

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Electrospinning Of Poly (Ester Amide) Fibres For Mesenchymal Progenitor Cell Differentiation, Sarah Kiros 2016 The University of Western Ontario

Electrospinning Of Poly (Ester Amide) Fibres For Mesenchymal Progenitor Cell Differentiation, Sarah Kiros

Electronic Thesis and Dissertation Repository

The in vitro vascular tissue engineering paradigm seeks to produce biologically responsive vascular substitutes using cells, biodegradable scaffolds, and bioreactors to mature the tissue for the potential treatment of vascular occlusions and to create 3D tissue models for pre-clinical testing. In this work, a poly (ester amide) (PEA) derived from from L-phenylalanine, sebacoyl chloride and 1,4 butanediol was synthesized and electrospun to form both 3D fibrous mats and tubular constructs. Both the polymer solution concentration and mandrel rotation speed were optimized to fabricate bead-free fibres. Cytocompatibility and proliferation studies using mesenchymal progenitor 10T1/2 cells showed PEA fibres were ...


Surface Engineering Of Titanium Alloy To Stimulate The Bone Regeneration, Yubin Meng, Zhenduo Cui, Zhaoyang Li, Xue Li, Jin Zhao, Xubo Yuan, Xianjin Yang 2016 Tianjin University

Surface Engineering Of Titanium Alloy To Stimulate The Bone Regeneration, Yubin Meng, Zhenduo Cui, Zhaoyang Li, Xue Li, Jin Zhao, Xubo Yuan, Xianjin Yang

The 8th International Conference on Physical and Numerical Simulation of Materials Processing

No abstract provided.


Stem Cell Based Tissue Engineering And Regenerative Medicine: A Review Focusing On Adult Stem Cells, Jordan Greenberg, Veronica R. Fortino, Daniel Pelaez, Herman S. Cheung 2016 University of Miami

Stem Cell Based Tissue Engineering And Regenerative Medicine: A Review Focusing On Adult Stem Cells, Jordan Greenberg, Veronica R. Fortino, Daniel Pelaez, Herman S. Cheung

Veronica Fortino

Tissue engineering has emerged as a field that attempts to harness the bodies' own developmental and repair features to treat diseases and illnesses. Many of these illnesses are caused by necrosis or loss of functionality of complete organs or specific cell types. Early discoveries in embryonic stem cells fueled a wave of research that led to claims about possibly regenerating nonfunctioning organs. Although we are still far away from being able to grow functional organs in a Petri dish, the field continues to progress forward, and new clinical trials have been approved for using both embryonic and adult stem cell ...


Adipose-Derived Stem Cell Differentiation In Cell Aggregates Supplemented With Micronized, Decellularized Extracellular Matrix, Danielle Heinbuch 2016 The University of Western Ontario

Adipose-Derived Stem Cell Differentiation In Cell Aggregates Supplemented With Micronized, Decellularized Extracellular Matrix, Danielle Heinbuch

Electronic Thesis and Dissertation Repository

In the human body there are tissues with limited regenerative potential, raising the need for cell-based regenerative therapies. The main objective of this thesis was to examine how tissue-specific extracellular matrix (ECM) affects the differentiation of human adipose-derived stem/stromal cells (ASCs). The effects of incorporating milled, decellularized adipose (DAT) or cartilage (DCT) ECM particles on ASC lineage-specific differentiation in 3-D cell aggregate cultures were explored. The results demonstrated that the addition of ECM improved differentiation for both adipogenesis and chondrogenesis. Analysis of adipogenic gene and protein expression indicated enhanced differentiation in the DAT+ASC aggregates relative to the DCT ...


Lysis And Amplifciation Of Neonatal Sepsis Causing Pathogens, Gregory Berglund, Elizabeth A. Phillips, Jacqueline C. Linnes 2016 Purdue University

Lysis And Amplifciation Of Neonatal Sepsis Causing Pathogens, Gregory Berglund, Elizabeth A. Phillips, Jacqueline C. Linnes

The Summer Undergraduate Research Fellowship (SURF) Symposium

Neonatal sepsis, resulting from a bloodstream infection within the first few weeks of life, is the leading cause of newborn deaths worldwide. The gold standard of neonatal sepsis diagnosis requires a blood culture to identify the infecting bacteria, however require days of incubation, expensive equipment, and expertise. Any delay in diagnosis is critical, as the condition can be treated easily if appropriate antibiotics are administered promptly. A low-cost, rapid, and sensitive diagnostic test would enable more timely treatment and lead to better patient outcomes with fewer required resources. Point-of-care, nucleic acid amplification assays are a promising alternative to blood culture ...


Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, Evan Surma, Sherry L. Harbin, Hongji Zhang, Stacy Halum 2016 Purdue University

Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, Evan Surma, Sherry L. Harbin, Hongji Zhang, Stacy Halum

The Summer Undergraduate Research Fellowship (SURF) Symposium

Lower back pain from intervertebral disc injury affects around 84% of the population at some point in their life, which at its worst may cause total immobilization. This pain can only be temporarily relieved by spinal fusion or intervertebral disc replacement; however, both of these cause loss of natural motion in patients by removing damaged fibrocartilage discs. While these techniques help mitigate pain briefly, no permanent solution exists currently to both relieve pain and preserve natural motion. My work may be a solution by eventually providing patient-specific implants that resemble native tissue in the regeneration process that could be absorbed ...


Bone Tissue Engineering: Scalability And Optimization Of Densified Collagen-Fibril Bone Graft Substitute Materials, John G. Nicholas, Lauren E. Watkins, Sherry L. Voytik-Harbin 2016 Purdue University

Bone Tissue Engineering: Scalability And Optimization Of Densified Collagen-Fibril Bone Graft Substitute Materials, John G. Nicholas, Lauren E. Watkins, Sherry L. Voytik-Harbin

The Summer Undergraduate Research Fellowship (SURF) Symposium

Over 240 million people missing teeth worldwide experience lingering problems such as difficulty speaking and eating, undesirable aesthetics, and resorption of bone supporting neighboring teeth. The gold standard of treatment utilizes grafts to attach a function-restoring implant to supporting bone. Current graft materials suffer from problems including autologous donor site morbidity, long resorption time, incomplete integration with the maxillae or mandible, and structural weakness. Patient-specific, cellularized bone grafts may be a solution to these issues by accelerating and improving the quality of regenerated bone. Recently, encapsulation of mesenchymal stem cells within self-assembling type I collagen oligomer matrices has been shown ...


Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song 2016 University of Massachusetts Medical School

Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song

Jie Song

Biodegradable polymers have been widely utilized as drug delivery vehicles and tissue engineering scaffolds. We previously designed amphiphilic triblock copolymer poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) (PELA) and its hydroxyapatite (HA) composites for bone tissue engineering applications. The hydrophilic electrospun PELA-HA composite exhibited aqueous stability and elastic handling characteristics, and was able to template the proliferation and osteogenesis of bone marrow stromal cells (BMSCs) in vitro and in vivo when spiral-wrapped into cylinders and press-fit into critical size femoral segmental defects in rats. However, the slow degradation of PELA has prevented timely disappearance of the scaffold and ...


Calcium Phosphate As A Key Material For Socially Responsible Tissue Engineering, Vuk Uskoković, Victoria M. Wu 2016 Chapman University

Calcium Phosphate As A Key Material For Socially Responsible Tissue Engineering, Vuk Uskoković, Victoria M. Wu

Pharmacy Faculty Articles and Research

Socially responsible technologies are designed while taking into consideration the socioeconomic, geopolitical and environmental limitations of regions in which they will be implemented. In the medical context, this involves making therapeutic platforms more accessible and affordable to patients in poor regions of the world wherein a given disease is endemic. This often necessitates going against the reigning trend of making therapeutic nanoparticles ever more structurally complex and expensive. However, studies aimed at simplifying materials and formulations while maintaining the functionality and therapeutic response of their more complex counterparts seldom provoke a significant interest in the scientific community. In this review ...


Lipoaspirate And Adipose Stem Cells As Potential Therapeutics For Chronic Scars, Dylan Perry, Jorge Lujan-Hernandez, Michael S. Chin, So-Yun Min, Ava Chappell, Raziel Rojas-Rodriguez, Raghu Appasani, Patrick Teebagy, Silvia Corvera, Janice F. Lalikos 2016 University of Massachusetts Medical School

Lipoaspirate And Adipose Stem Cells As Potential Therapeutics For Chronic Scars, Dylan Perry, Jorge Lujan-Hernandez, Michael S. Chin, So-Yun Min, Ava Chappell, Raziel Rojas-Rodriguez, Raghu Appasani, Patrick Teebagy, Silvia Corvera, Janice F. Lalikos

UMass Center for Clinical and Translational Science Research Retreat

Introduction: Burn injuries can lead to hypertrophic or keloid scars, causing pain and long lasting mobility issues. Current therapies are often unsatisfactory, costly, or morbid. Prior studies suggest adipose derived stem cells (ADSCs) and lipoaspirate can improve scar outcomes of acute thermal wounds. Clinical reports suggest lipoaspirate and ADSCs can improve chronic burn scar remodeling. However, this has not been extensively studied in animal models. We sought to determine if adipose tissue can improve chronic scar remodeling and to compare the effects of ADSCs and processed lipoaspirate.

Methods: 50 CD1 nu/nu athymic mice received a standardized deep partial-thickness thermal ...


Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song 2016 University of Massachusetts Medical School

Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song

UMass Center for Clinical and Translational Science Research Retreat

Biodegradable polymers have been widely utilized as drug delivery vehicles and tissue engineering scaffolds. We previously designed amphiphilic triblock copolymer poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) (PELA) and its hydroxyapatite (HA) composites for bone tissue engineering applications. The hydrophilic electrospun PELA-HA composite exhibited aqueous stability and elastic handling characteristics, and was able to template the proliferation and osteogenesis of bone marrow stromal cells (BMSCs) in vitro and in vivo when spiral-wrapped into cylinders and press-fit into critical size femoral segmental defects in rats. However, the slow degradation of PELA has prevented timely disappearance of the scaffold and ...


Implantable Microenvironments To Capture Stable-To- Aggressive Tumor Transition, Ryan Carpenter, Jungwoo Lee 2016 University of Massachusetts Amherst

Implantable Microenvironments To Capture Stable-To- Aggressive Tumor Transition, Ryan Carpenter, Jungwoo Lee

UMass Center for Clinical and Translational Science Research Retreat

Clinical stability occurs when cancers reach a state where the disease neither advances nor regresses. Tumors can remain in this state for multiple years before progressing to more aggressive phenotypes. The mechanisms for maintaining a stable state and the factors that contribute to tumor activation are poorly understood. We hypothesized that an implantable biomaterial scaffold would be able to isolate a population of stable tumor cells that could then be used to study the transition to an aggressive phenotype. In this work we developed a tunable and highly controlled, porous acrylamide scaffold and subcutaneously implanted them in immunodeficient (NSG) mice ...


Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez 2016 Augustana College, Rock Island Illinois

Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez

Biology: Student Scholarship & Creative Works

ABSTRACT: The HIV-1 pandemic continues to thrive due to ineffective HIV-1 vaccines. Historically, the world’s most infectious diseases, such as polio and smallpox, have been eradicated or have come close to eradication due to the advent of effective vaccines. Highly active antiretroviral therapy is able to delay the onset of AIDS but can neither rid the body of HIV-1 proviral DNA nor prevent further transmission. A prophylactic vaccine that prevents the various mechanisms HIV-1 has to evade and attack our immune system is needed to end the HIV-1 pandemic. Recent advances in engineered nuclease systems, like the CRISPR/Cas9 ...


Interaction Of Fibrinogen With Fibronectin: Purification And Characterization Of A Room Temperature-Stable Fibrinogen-Fibronectin Complex From Normal Human Plasma, Ayman E. Ismail 2016 University of Nebraska-Lincoln

Interaction Of Fibrinogen With Fibronectin: Purification And Characterization Of A Room Temperature-Stable Fibrinogen-Fibronectin Complex From Normal Human Plasma, Ayman E. Ismail

Chemical & Biomolecular Engineering Theses, Dissertations, & Student Research

A fibrinogen-fibronectin complex (γγ’pdFI-pdFN) was purified from normal human plasma using a sequence of cryoprecipitation, ammonium sulfate fractionation, and DEAE Sepharose chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing condition showed both a 1:1 stoichiometric ratio of fibrinogen (FI) to fibronectin (FN) as well as a stoichiometric ratio of 1:1 of γg to gγ’. The γγ’pdFI-pdFN complex was non-covalent in nature as it was disrupted by affinity adsorption to Gelatin Sepharose where pdFN bound strongly and the disrupted γγ’pdFI fell through the chromatographic column. Surprisingly, the purified γγ’pdFI-pdFN complex was more broadly ...


Inter-Droplet Membranes For Mechanical Sensing Applications, Nima Tamaddoni Jahromi 2016 University of Tennessee - Knoxville

Inter-Droplet Membranes For Mechanical Sensing Applications, Nima Tamaddoni Jahromi

Doctoral Dissertations

This dissertation combines self-assembly phenomena of amphiphilic molecules with soft materials to create and characterize mechanoelectrical transducers and sensors whose sensing elements are thin-film bioinspired membranes comprised of phospholipids or amphiphilic polymers. We show that the structures of these amphiphilic molecules tune the mechanical and electrical properties of these membranes. We show that these properties affect the mechanoelectrical sensing characteristic and range of operation of these membrane transducers. In the experiments, we construct and characterize a membrane-based hair cell embodiment that enables the membrane to be responsive to mechanical perturbations of the hair. The resulting oscillations of membranes formed between ...


Exploring Biomarkers For Point Of Care Bladder Cancer Detection, Cassandra M. Walker 2016 University of Arkansas, Fayetteville

Exploring Biomarkers For Point Of Care Bladder Cancer Detection, Cassandra M. Walker

Biomedical Engineering Undergraduate Honors Theses

Bladder cancer is the 5th most common non-cutaneous human cancer in the United States. While effective methods of detecting bladder cancer are currently practiced, they are often expensive and invasive. There is a need for a noninvasive detection method that can be used in areas with few medical resources. Cell free DNA in urine is normally present only in very low concentrations. Abnormally high levels of cell free DNA in urine could be indicative of disease. This study tests the hypothesis that DNA present in urine can be used as a biomarker for bladder cancer before hematuria is seen in ...


Sustained Cell Differentiation Of 2d H9 Human Embryonic Stem Cells Into Mesenchymal Stem Cells, Hannah M. Christian 2016 University of Nebraska-Lincoln

Sustained Cell Differentiation Of 2d H9 Human Embryonic Stem Cells Into Mesenchymal Stem Cells, Hannah M. Christian

UCARE Research Products

This experiment consisted of the controlled differentiation of H9 embryonic stem cells to mesenchymal stem cells. Though this experiment was repeated twice and improvement was seen in these repetitions, the cells were only able to be partially differentiated. However, the morphology of the differentiated cells is similar to those of healthy adult mesenchymal stem cells.

The progression of the differentiation can be seen in the microscope slide photos below. Throughout the differentiation, there occurred a decrease in cell survival and reduction of cell growth, but an increase in mesenchymal stem cell morphology. Throughout the last week of the differentiation, very ...


Pnipam Particles For Protein Delivery To Tumor-Associated Macrophages., Christopher Isely 2016 Iowa State University

Pnipam Particles For Protein Delivery To Tumor-Associated Macrophages., Christopher Isely

Honors Projects and Posters

Drug delivery vehicles are designed to address limitations associated with traditional drug administration methods. In the case of cancer immunotherapy, the problem often lies in toxicity of drug, local delivery and short residence time in the body. In cancer, macrophages are prime targets for immunotherapy. They exist on a spectrum of phenotypes, with M1 classically activated and M2 alternatively activated macrophages being the traditional ends of this spectrum. M2 macrophages are pro-wound healing, and M1 macrophages are pro-inflammatory. Tumor associated macrophages (TAM’s) are mostly M2-like macrophages and promote tumor growth. Interleukin 12 (IL-12) delivery to macrophages has been shown ...


Patterned Alginate Hydrogels To Induce Chondrocyte Alignment, Jordan Catherine Verplank, Taylor D. Laughlin, Angela K. Pannier 2016 University of Nebraska-Lincoln

Patterned Alginate Hydrogels To Induce Chondrocyte Alignment, Jordan Catherine Verplank, Taylor D. Laughlin, Angela K. Pannier

UCARE Research Products

The growth plate has an intricate architecture, and this architecture is necessary for directional growth of bones. Specifically, the cells align in longitudinal columns. As the growth plate expands with this pattern, the bone elongates with the same alignment pattern. The purpose of this research is to mimic this single celled, columnar alignment in vitro. In developing this alignment in vitro, this research will contribute to the overall study of growing growth for the development of improved therapeutic treatments and engineered tissues for transplants.


Hyaluronic Acid-Conjugated Liposome Nanoparticles For Targeted Delivery To Cd44 Overexpressing Glioblastoma Cells, Stephen L. Hayward, Christina L. Wilson, Srivatsan Kidambi 2016 University of Nebraska-Lincoln

Hyaluronic Acid-Conjugated Liposome Nanoparticles For Targeted Delivery To Cd44 Overexpressing Glioblastoma Cells, Stephen L. Hayward, Christina L. Wilson, Srivatsan Kidambi

Chemical and Biomolecular Engineering -- All Faculty Papers

Glioblastoma Multiforme (GBM) is a highly prevalent and deadly brain malignancy characterized by poor prognosis and restricted disease management potential. Despite the success of nanocarrier systems to improve drug/gene therapy for cancer, active targeting specificity remains a major hurdle for GBM. Additionally, since the brain is a multi-cell type organ, there is a critical need to develop an approach to distinguish between GBM cells and healthy brain cells for safe and successful treatment. In this report, we have incorporated hyaluronic acid (HA) as an active targeting ligand for GBM. To do so, we employed HA conjugated liposomes (HALNPs) to ...


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