Calcium Phosphate As A Key Material For Socially Responsible Tissue Engineering, 2016 Chapman University
Calcium Phosphate As A Key Material For Socially Responsible Tissue Engineering, Vuk Uskoković, Victoria M. Wu
Pharmacy Faculty Articles and Research
Socially responsible technologies are designed while taking into consideration the socioeconomic, geopolitical and environmental limitations of regions in which they will be implemented. In the medical context, this involves making therapeutic platforms more accessible and affordable to patients in poor regions of the world wherein a given disease is endemic. This often necessitates going against the reigning trend of making therapeutic nanoparticles ever more structurally complex and expensive. However, studies aimed at simplifying materials and formulations while maintaining the functionality and therapeutic response of their more complex counterparts seldom provoke a significant interest in the scientific community. In this review ...
Implantable Microenvironments To Capture Stable-To- Aggressive Tumor Transition, 2016 University of Massachusetts Amherst
Implantable Microenvironments To Capture Stable-To- Aggressive Tumor Transition, Ryan Carpenter, Jungwoo Lee
UMass Center for Clinical and Translational Science Research Retreat
Clinical stability occurs when cancers reach a state where the disease neither advances nor regresses. Tumors can remain in this state for multiple years before progressing to more aggressive phenotypes. The mechanisms for maintaining a stable state and the factors that contribute to tumor activation are poorly understood. We hypothesized that an implantable biomaterial scaffold would be able to isolate a population of stable tumor cells that could then be used to study the transition to an aggressive phenotype. In this work we developed a tunable and highly controlled, porous acrylamide scaffold and subcutaneously implanted them in immunodeficient (NSG) mice ...
Lipoaspirate And Adipose Stem Cells As Potential Therapeutics For Chronic Scars, 2016 University of Massachusetts Medical School
Lipoaspirate And Adipose Stem Cells As Potential Therapeutics For Chronic Scars, Dylan Perry, Jorge Lujan-Hernandez, Michael S. Chin, So-Yun Min, Ava Chappell, Raziel Rojas-Rodriguez, Raghu Appasani, Patrick Teebagy, Silvia Corvera, Janice F. Lalikos
UMass Center for Clinical and Translational Science Research Retreat
Introduction: Burn injuries can lead to hypertrophic or keloid scars, causing pain and long lasting mobility issues. Current therapies are often unsatisfactory, costly, or morbid. Prior studies suggest adipose derived stem cells (ADSCs) and lipoaspirate can improve scar outcomes of acute thermal wounds. Clinical reports suggest lipoaspirate and ADSCs can improve chronic burn scar remodeling. However, this has not been extensively studied in animal models. We sought to determine if adipose tissue can improve chronic scar remodeling and to compare the effects of ADSCs and processed lipoaspirate.
Methods: 50 CD1 nu/nu athymic mice received a standardized deep partial-thickness thermal ...
Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, 2016 University of Massachusetts Medical School
Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song
UMass Center for Clinical and Translational Science Research Retreat
Biodegradable polymers have been widely utilized as drug delivery vehicles and tissue engineering scaffolds. We previously designed amphiphilic triblock copolymer poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) (PELA) and its hydroxyapatite (HA) composites for bone tissue engineering applications. The hydrophilic electrospun PELA-HA composite exhibited aqueous stability and elastic handling characteristics, and was able to template the proliferation and osteogenesis of bone marrow stromal cells (BMSCs) in vitro and in vivo when spiral-wrapped into cylinders and press-fit into critical size femoral segmental defects in rats. However, the slow degradation of PELA has prevented timely disappearance of the scaffold and ...
Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, 2016 Augustana College, Rock Island Illinois
Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez
Biology: Student Scholarship & Creative Works
ABSTRACT: The HIV-1 pandemic continues to thrive due to ineffective HIV-1 vaccines. Historically, the world’s most infectious diseases, such as polio and smallpox, have been eradicated or have come close to eradication due to the advent of effective vaccines. Highly active antiretroviral therapy is able to delay the onset of AIDS but can neither rid the body of HIV-1 proviral DNA nor prevent further transmission. A prophylactic vaccine that prevents the various mechanisms HIV-1 has to evade and attack our immune system is needed to end the HIV-1 pandemic. Recent advances in engineered nuclease systems, like the CRISPR/Cas9 ...
Interaction Of Fibrinogen With Fibronectin: Purification And Characterization Of A Room Temperature-Stable Fibrinogen-Fibronectin Complex From Normal Human Plasma, Ayman E. Ismail
Chemical & Biomolecular Engineering Theses, Dissertations, & Student Research
A fibrinogen-fibronectin complex (γγ’pdFI-pdFN) was purified from normal human plasma using a sequence of cryoprecipitation, ammonium sulfate fractionation, and DEAE Sepharose chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing condition showed both a 1:1 stoichiometric ratio of fibrinogen (FI) to fibronectin (FN) as well as a stoichiometric ratio of 1:1 of γg to gγ’. The γγ’pdFI-pdFN complex was non-covalent in nature as it was disrupted by affinity adsorption to Gelatin Sepharose where pdFN bound strongly and the disrupted γγ’pdFI fell through the chromatographic column. Surprisingly, the purified γγ’pdFI-pdFN complex was more broadly ...
Inter-Droplet Membranes For Mechanical Sensing Applications, 2016 University of Tennessee - Knoxville
Inter-Droplet Membranes For Mechanical Sensing Applications, Nima Tamaddoni Jahromi
This dissertation combines self-assembly phenomena of amphiphilic molecules with soft materials to create and characterize mechanoelectrical transducers and sensors whose sensing elements are thin-film bioinspired membranes comprised of phospholipids or amphiphilic polymers. We show that the structures of these amphiphilic molecules tune the mechanical and electrical properties of these membranes. We show that these properties affect the mechanoelectrical sensing characteristic and range of operation of these membrane transducers. In the experiments, we construct and characterize a membrane-based hair cell embodiment that enables the membrane to be responsive to mechanical perturbations of the hair. The resulting oscillations of membranes formed between ...
Exploring Biomarkers For Point Of Care Bladder Cancer Detection, 2016 University of Arkansas, Fayetteville
Exploring Biomarkers For Point Of Care Bladder Cancer Detection, Cassandra M. Walker
Biomedical Engineering Undergraduate Honors Theses
Bladder cancer is the 5th most common non-cutaneous human cancer in the United States. While effective methods of detecting bladder cancer are currently practiced, they are often expensive and invasive. There is a need for a noninvasive detection method that can be used in areas with few medical resources. Cell free DNA in urine is normally present only in very low concentrations. Abnormally high levels of cell free DNA in urine could be indicative of disease. This study tests the hypothesis that DNA present in urine can be used as a biomarker for bladder cancer before hematuria is seen in ...
Sustained Cell Differentiation Of 2d H9 Human Embryonic Stem Cells Into Mesenchymal Stem Cells, 2016 University of Nebraska-Lincoln
Sustained Cell Differentiation Of 2d H9 Human Embryonic Stem Cells Into Mesenchymal Stem Cells, Hannah M. Christian
UCARE Research Products
This experiment consisted of the controlled differentiation of H9 embryonic stem cells to mesenchymal stem cells. Though this experiment was repeated twice and improvement was seen in these repetitions, the cells were only able to be partially differentiated. However, the morphology of the differentiated cells is similar to those of healthy adult mesenchymal stem cells.
The progression of the differentiation can be seen in the microscope slide photos below. Throughout the differentiation, there occurred a decrease in cell survival and reduction of cell growth, but an increase in mesenchymal stem cell morphology. Throughout the last week of the differentiation, very ...
Patterned Alginate Hydrogels To Induce Chondrocyte Alignment, 2016 University of Nebraska-Lincoln
Patterned Alginate Hydrogels To Induce Chondrocyte Alignment, Jordan Catherine Verplank, Taylor D. Laughlin, Angela K. Pannier
UCARE Research Products
The growth plate has an intricate architecture, and this architecture is necessary for directional growth of bones. Specifically, the cells align in longitudinal columns. As the growth plate expands with this pattern, the bone elongates with the same alignment pattern. The purpose of this research is to mimic this single celled, columnar alignment in vitro. In developing this alignment in vitro, this research will contribute to the overall study of growing growth for the development of improved therapeutic treatments and engineered tissues for transplants.
Pnipam Particles For Protein Delivery To Tumor-Associated Macrophages., 2016 Iowa State University
Pnipam Particles For Protein Delivery To Tumor-Associated Macrophages., Christopher Isely
Honors Projects and Posters
Drug delivery vehicles are designed to address limitations associated with traditional drug administration methods. In the case of cancer immunotherapy, the problem often lies in toxicity of drug, local delivery and short residence time in the body. In cancer, macrophages are prime targets for immunotherapy. They exist on a spectrum of phenotypes, with M1 classically activated and M2 alternatively activated macrophages being the traditional ends of this spectrum. M2 macrophages are pro-wound healing, and M1 macrophages are pro-inflammatory. Tumor associated macrophages (TAM’s) are mostly M2-like macrophages and promote tumor growth. Interleukin 12 (IL-12) delivery to macrophages has been shown ...
Equilibrium Testing Of Rat Tail Tendon: An Analysis Of The Viscoelastic Properties Of Collagen Under Different Strain Points, 2016 Rose-Hulman Institute of Technology
Equilibrium Testing Of Rat Tail Tendon: An Analysis Of The Viscoelastic Properties Of Collagen Under Different Strain Points, Joshua C. Witt
Graduate Theses - Biology & Biomedical Engineering
Instantaneous tensile testing and stress-relaxation testing are forms of mechanical testing used to determine the elastic and viscoelastic properties of biological tissue. Equilibrium testing is a form of testing that combines both of these testing approaches at different strain points to determine the elastic properties of a material and also assess their viscoelastic properties in the same test. This testing method is commonly used on highly viscoelastic materials such as cartilage but has never been fully described in dense collagenous materials such as tendon or ligament. This analysis utilizes different strain points selected to capture the classic non-linear behavior of ...
Novel Small Airway Model Using Electrospun Decellularized Lung Extracellular Matrix, Bethany M. Young
Theses and Dissertations
Chronic respiratory diseases affects many people worldwide with little known about the mechanisms diving the pathology, making it difficult to find a cure. Improving the understanding of smooth muscle and extracellular matrix (ECM) interaction is key to developing a remedy to this leading cause of death. With currently no relevant or controllable in vivo or in vitro model to investigate diseased and normal interactions of small airway components, the development of a physiologically relevant in vitro model with comparable cell attachment, signaling, and organization is necessary to develop new treatments for airway disease. The goal of this study is to ...
A Scalable Low-Cost Cgmp Process For Clinical Grade Production Of The Hiv Inhibitor 5p12-Rantes In Pichia Pastoris, 2016 University of Geneva, Geneva, Switzerland
A Scalable Low-Cost Cgmp Process For Clinical Grade Production Of The Hiv Inhibitor 5p12-Rantes In Pichia Pastoris, Fabrice Cerini, Hubert Gaertner, Knut Madden, Ilya Tolstorukov, Scott Brown, Bram Laukens, Nico Callewaert, Jay C. Harner, Anna M. Oommen, John T. Harms, Anthony R. Sump, Robert C. Sealock, Dustin J. Peterson, Scott K. Johnson, Stephan B. Abramson, Michael M. Meagher, Robin Offord, Oliver Hartley
Chemical and Biomolecular Engineering -- All Faculty Papers
In the continued absence of an effective anti-HIV vaccine, approximately 2 million new HIV infections occur every year, with over 95% of these in developing countries. Calls have been made for the development of anti-HIV drugs that can be formulated for topical use to prevent HIV transmission during sexual intercourse. Because these drugs are principally destined for use in low-resource regions, achieving production costs that are as low as possible is an absolute requirement. 5P12-RANTES, an analog of the human chemokine protein RANTES/CCL5, is a highly potent HIV entry inhibitor which acts by achieving potent blockade of the principal ...
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, 2015 Worcester Polytechnic Institute
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Olga Kashpur, David Lapointe, Sakthikumar Ambady, Elizabeth Ryder, Tanja Dominko
BACKGROUND: Adult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury - by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent (iRC) fibroblasts to identify transcriptional changes that may be related to their regeneration competence. RESULTS: We identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion molecules ...
Next-Generation Mrna Sequencing Reveals Pyroptosis-Induced Cd4+ T Cell Death In Early Simian Immunodeficiency Virus-Infected Lymphoid Tissues, 2015 University of Nebraska—Lincoln
Next-Generation Mrna Sequencing Reveals Pyroptosis-Induced Cd4+ T Cell Death In Early Simian Immunodeficiency Virus-Infected Lymphoid Tissues, Wuxun Lu, Andrew J. Demers, Fangrui Ma, Guobin Kang, Zhe Yuan, Yanmin Wan, Yue Li, Jiangqing Xu, Mark Lewis, Qingsheng Li
Papers from the Nebraska Center for Biotechnology
Lymphoid tissues (LTs) are the principal sites where human immunodeficiency virus type 1 (HIV-1) replicates and virus-host interactions take place, resulting in immunopathology in the form of inflammation, immune activation, and CD4+ T cell death. The HIV-1 pathogenesis in LTs has been extensively studied; however, our understanding of the virus-host interactions in the very early stages of infection remains incomplete. We investigated virus-host interactions in the rectal draining lymph nodes (dLNs) of rhesus macaques at different times after intrarectal inoculation (days postinoculation [dpi]) with simian immunodeficiency virus (SIV). At 3 dpi, 103 differentially expressed genes (DEGs) were detected using next-generation ...
Incorporation Of Fibrin Into A Collagen–Glycosaminoglycan Matrix Results In A Scaffold With Improved Mechanical Properties And Enhanced Capacity To Resist Cell-Mediated Contraction, 2015 Dublin Institute of Technology
Incorporation Of Fibrin Into A Collagen–Glycosaminoglycan Matrix Results In A Scaffold With Improved Mechanical Properties And Enhanced Capacity To Resist Cell-Mediated Contraction, Claire Brougham, Tanya J. Levingstone, Stefan Jockenhoevel, Thomas C. Flanagan, Fergal J. O'Brien
Fibrin has many uses as a tissue engineering scaffold, however many in vivo studies have shown a reduction in function resulting from the susceptibility of fibrin to cell-mediated contraction. The overall aim of the present study was to develop and characterise a reinforced natural scaffold using fibrin, collagen and glycosaminoglycan (FCG), and to examine the cell-mediated contraction of this scaffold in comparison to fibrin gels. Through the use of an injection loading technique, a homogenous FCG scaffold was developed. Mechanical testing showed a sixfold increase in compressive modulus and a thirtyfold increase in tensile modulus of fibrin when reinforced with ...
Investigation Of Bovine Serum Albumin (Bsa) Attachment Onto Self-Assembled Monolayers (Sams) Using Combinatorial Quartz Crystal Microbalance With Dissipation (Qcm-D) And Spectroscopic Ellipsometry (Se), 2015 University of Nebraska-Lincoln
Investigation Of Bovine Serum Albumin (Bsa) Attachment Onto Self-Assembled Monolayers (Sams) Using Combinatorial Quartz Crystal Microbalance With Dissipation (Qcm-D) And Spectroscopic Ellipsometry (Se), Hanh T.M. Phan, Shannon L. Bartelt-Hunt, Keith B. Rodenhausen, Mathias Schubert, Jason C. Bartz
Civil Engineering Faculty Publications
Understanding protein adsorption kinetics to surfaces is of importance for various environmental and biomedical applications. Adsorption of bovine serum albumin to various selfassembled monolayer surfaces including neutral and charged hydrophilic and hydrophobic surfaces was investigated using in-situ combinatorial quartz crystal microbalance with dissipation and spectroscopic ellipsometry. Adsorption of bovine serum albumin varied as a function of surface properties, bovine serum albumin concentration and pH value. Charged surfaces exhibited a greater quantity of bovine serum albumin adsorption, a larger bovine serum albumin layer thickness, and increased density of bovine serum albumin protein compared to neutral surfaces at neutral pH value. The ...
Three-Dimensional Confocal Microscopy Indentation Method For Hydrogel Elasticity Measurement, 2015 University of Nebraska-Lincoln
Three-Dimensional Confocal Microscopy Indentation Method For Hydrogel Elasticity Measurement, Donghee Lee, Md Mahmudur Rahman, You Zhou, Sangjin Ryu
Md Mahmudur Rahman
No abstract provided.
Bioengineered Cell Niche For Skeletal Muscle Regeneration, 2015 Purdue University
Bioengineered Cell Niche For Skeletal Muscle Regeneration, Nicole M. Whittern, Naagarajan Narayanan, Chunhui Jiang, Owen Jones, Jay Gilbert, Michael Whittern, Shihuan Kuang, Meng Deng
The Summer Undergraduate Research Fellowship (SURF) Symposium
Skeletal muscles can self-repair minor strains, lacerations, and contusions; however, in cases of volumetric muscle lossand muscle degenerative diseases, tissue fails to regenerate. Current cell-based therapies, such as myoblast transplantation, have significant drawbacks of low survival rates and engraftment efficacy, mainly due to the absence of supportive cell microenvironment. Scaffolds that mimic the natural cell microenvironment provide a robust platform to support cell adhesion, migration, proliferation, and differentiation. Electrospinning is a versatile technology platform used for fabricating the fiber scaffold that mimics the extracellular matrix. Thus, we aim to reconstitute the cell microenvironment through development of aligned fiber scaffolds by ...