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Molecular, Cellular, and Tissue Engineering Commons

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319 full-text articles. Page 1 of 13.

Influence Of Fibroblasts On Functional Arteriogenesis In A Murine Chronic Hindlimb Ischemia Model, Ashli A. Santos 2017 California Polytechnic State University, San Luis Obispo

Influence Of Fibroblasts On Functional Arteriogenesis In A Murine Chronic Hindlimb Ischemia Model, Ashli A. Santos

Biomedical Eng/General Eng.

Peripheral arterial occlusive disease (PAOD) occurs when there is a narrowing or blockage – usually a buildup of plaque - within the arteries that reduces blood flow to tissues which can chronic ischemia. As with most diseases, early detection and proactive treatment are important to maximize prognosis. Exercise effectively treats PAOD, but due to ischemic pain in the limbs, or intermittent claudication (IC), exercise can become painful and difficult. Due to the buildup of plaque, occlusions create an ischemic environment that changes the pressure distribution in collateral networks and increases the shear stress in transverse collaterals. Those two responses signal the beginning ...


A Spatial Stochastic Model Of Ampar Trafficking And Subunit Dynamics, Tyler VanDyk, Matthew C. Pharris, Tamara L. Kinzer-Ursem 2017 Purdue University

A Spatial Stochastic Model Of Ampar Trafficking And Subunit Dynamics, Tyler Vandyk, Matthew C. Pharris, Tamara L. Kinzer-Ursem

The Summer Undergraduate Research Fellowship (SURF) Symposium

In excitatory neurons, the ability of a synaptic connection to strengthen or weaken is known as synaptic plasticity and is thought to be the cellular basis for learning and memory. Understanding the mechanism of synaptic plasticity is an important step towards understanding and developing treatment methods for learning and memory disorders. A key molecular process in synaptic plasticity for mammalian glutamatergic neurons is the exocytosis (delivery to the synapse) of AMPA-type glutamate receptors (AMPARs). While the protein signaling pathways responsible for exocytosis have long been investigated with experimental methods, it remains unreasonable to study the system in its full complexity ...


A Novel High-Throughput, High-Content Three-Dimensional Assay For Determination Of Tumor Invasion And Dormancy, Mahera M. Husain, Theodore J. Puls, Sherry Voytik-Harbin 2017 Purdue University

A Novel High-Throughput, High-Content Three-Dimensional Assay For Determination Of Tumor Invasion And Dormancy, Mahera M. Husain, Theodore J. Puls, Sherry Voytik-Harbin

The Summer Undergraduate Research Fellowship (SURF) Symposium

Metastasis accounts for most cancer deaths, while dormancy of tumor cells leads to unexpected cancer recurrence. These two aspects of cancer remain relatively untreatable in part because current two-dimensional (2D) methods of high-throughput drug screening cannot quantify outcomes related to these phenotypes. Three-dimensional (3D) in-vitro tumor models are a promising alternative because they better recreate the tumor microenvironment and relevant phenotypes. However, outcome measures for high-throughput screening of these systems are often limited to single measures such as metabolic activity using assays that are not standardized or optimized for 3D models. To address this gap, the objective of this work ...


Localized Immunosuppression Therapy For Islet Cell Encapsulation, Madeline McLaughlin, Clarissa Stephens, Sherry Voytik-Harbin 2017 Purdue University

Localized Immunosuppression Therapy For Islet Cell Encapsulation, Madeline Mclaughlin, Clarissa Stephens, Sherry Voytik-Harbin

The Summer Undergraduate Research Fellowship (SURF) Symposium

Type 1 diabetes, an autoimmune disease in which the body’s immune system destroys the insulin-producing beta cells necessary for managing a person’s blood glucose levels, affects 1.25 million Americans. A potential treatment for this disease is islet cell transplantation where Islets of Langerhans, containing the beta cells, are transplanted from a normal donor to a diabetic recipient to regulate blood glucose levels and provide insulin independence. Similar to whole organ transplantation, immune modulation through immunosuppression therapy is necessary for successful transplantation of islets without rejection. However, long-term systemic immunosuppression therapy can be toxic to the patient and ...


Multi-Color Ultra-High Resolution Imaging, David A. Miller, Michael Mlodzianoski, Sheng Liu, Fang Huang 2017 Purdue University

Multi-Color Ultra-High Resolution Imaging, David A. Miller, Michael Mlodzianoski, Sheng Liu, Fang Huang

The Summer Undergraduate Research Fellowship (SURF) Symposium

Fluorescence microscopy, which allows multiple-color imaging, plays an important role in observing structures inside cells with high specificity. The advent of super-resolution fluorescence microscopy, or nanoscopy techniques such as single-molecule switching nanoscopy (SMSN), has extended the application range of fluorescence microscopy beyond the diffraction limit, achieving up to 10-fold improvement in spatial resolution. At the same time, the recent development of expansion microscopy (ExM) allows samples to be physically expanded by 4-fold in the lateral dimensions providing another independent method to resolve structures beyond the diffraction limit. When combined, ExM-SMSN makes it possible to achieve another significant leap in resolution ...


Three-Dimensional Microfluidic Tumor Vascular Model For Investigating Breast Cancer Metastasis, Anastasiia Vasiukhina, Brian H. Jun, Luis Solorio, Pavlos P. Vlachos 2017 Miami University - Oxford

Three-Dimensional Microfluidic Tumor Vascular Model For Investigating Breast Cancer Metastasis, Anastasiia Vasiukhina, Brian H. Jun, Luis Solorio, Pavlos P. Vlachos

The Summer Undergraduate Research Fellowship (SURF) Symposium

Metastasis is one of the primary reasons for the high mortality rates in female patients diagnosed with breast cancer. It involves the migration of cancer cells into the circulatory system allowing for the dissemination of cancer cells in distal tissues. Understanding the major processes that occur in cells and tissues during metastasis can help improve currently existing therapeutic methods. In order to understand such mechanisms, developing physiologically relevant tissue models is crucial. Advancements in microfluidics have led to the fabrication of 3D culture models with shear stress gradients and flow control that can recapitulate aspects of the tumor microenvironment in ...


Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus 2017 Purdue University

Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus

The Summer Undergraduate Research Fellowship (SURF) Symposium

Benzene, a toxin and carcinogen found in air polluted by cigarette smoke, car exhaust, and industrial processes, is associated with the development of leukemia and lymphoma. Other than avoiding exposure, there is no current method to deter the effects of benzene. One potential strategy to prevent these effects is to engineer the bacteria of the human lung microbiome to degrade benzene. To evaluate this novel approach, we must verify that the bacteria remain viable within the lung microenvironment. To do so, lungs were harvested from rats and swabbed to determine the contents of the original lung microbiome. Then green fluorescent ...


Fret Biosensors: Engineering Fluorescent Proteins As Biological Tools For Studying Parkinson’S Disease, Nathan J. LeRoy, Jacob R. Norley, Saranya Radhakrishnan, Mathew Tantama 2017 Purdue University

Fret Biosensors: Engineering Fluorescent Proteins As Biological Tools For Studying Parkinson’S Disease, Nathan J. Leroy, Jacob R. Norley, Saranya Radhakrishnan, Mathew Tantama

The Summer Undergraduate Research Fellowship (SURF) Symposium

Parkinson’s Disease (PD) is a common neurodegenerative disease with over 200,000 new cases each year. In general, the cause of the disease is unknown, but oxidative stress inside of neurons has been associated with the disease’s pathology for some time. Currently, techniques to study the onset of PD inside of neurons are limited. This makes treatments and causes difficult to discover. One solution to this has been fluorescent protein biosensors. In short, these proteins can be engineered to glow when a certain state is achieved inside a cell. The present research discusses the engineering of a genetically-encoded ...


Intravenous Administration Of Iron Oxide Nanoparticles In The Chicken Model, Huong Thi Ngoc Le 2017 University of Arkansas, Fayetteville

Intravenous Administration Of Iron Oxide Nanoparticles In The Chicken Model, Huong Thi Ngoc Le

Theses and Dissertations

To be used in health care, the safety and effectiveness of nanoparticles needs to be tested in a living organism. The objective of this project was to develop the chicken as a convenient animal model to examine tissue targeting of intravenously (i.v.)-injected iron oxide (IO) nanoparticles. In Experiment 1, different doses of IO-COOH were i.v. injected into chickens; blood was collected at 0, 5, 15, 30, and 60 minutes post-injection; liver, spleen, lung, and kidney were collected after the last blood collection. For Experiment 2, IO-COOH and IO-PEG were i.v. injected into chickens; blood and the ...


The Impact Of The Mitochondrial Metabolism Of Induced Pluripotent Stem Cells Upon Differentiation, Stefanie T. Shahan 2017 Washington University

The Impact Of The Mitochondrial Metabolism Of Induced Pluripotent Stem Cells Upon Differentiation, Stefanie T. Shahan

Engineering and Applied Science Theses & Dissertations

Induced pluripotent stem cells (iPSCs) can be differentiated into any cell type found in the body. The derivation of a stem cell derived β cell (SC-β) capable of responding to glucose by secreting insulin was hugely significant for diabetes research and opened up the possibility of cell replacement therapy to combat this widespread disease (Pagliuca et al. 2014). The optimization of differentiation procedures such as this could improve yield, function, cost, and efficiency of a stem cell-derived product. Current approaches to improve differentiation are primarily focused on signal transduction pathways, while the metabolic state of the cells has received little ...


Mri Applications In Tissue Engineering, Shadi Othman 2017 University of the Pacific

Mri Applications In Tissue Engineering, Shadi Othman

Science Seminar Series

Shadi Othman of the School of Engineering and Computer Science Bioengineering Program, will speak on his research on MRI applications in tissue engineering.


Ultrasonically Responsive Tissue Engineering Scaffolds For The Temporal Control Over Osteo-Inductive Growth Factor Delivery, Catherine Linh 2017 University of Rhode Island

Ultrasonically Responsive Tissue Engineering Scaffolds For The Temporal Control Over Osteo-Inductive Growth Factor Delivery, Catherine Linh

Senior Honors Projects

In 2012, approximately 6.8 million people in the United States were diagnosed with orthopedic injuries or diseases. Over 500,000 people in the United States underwent bone grafting procedures, which cost 2.5 billion dollars per year and can result in complications. Polymer-based grafting scaffolds can facilitate 3D bone tissue growth in a localized, sustained manner. However, bone regeneration requires the orchestration of a sequence of events. Current scaffolds based on degradation and diffusion cannot provide sequential deliveries. We aimed to design a polymer scaffold that can release one payload diffusively at early time points, followed by ultrasonically triggered ...


Development And Evaluation Of A Biocompatible Electroactive Sensor For Continuous Blood Pressure Measurement., Scott D. Cambron 2017 University of Louisville

Development And Evaluation Of A Biocompatible Electroactive Sensor For Continuous Blood Pressure Measurement., Scott D. Cambron

Electronic Theses and Dissertations

Piezo-active composites have been implemented for sensing and transduction for decades. The 0-3 ceramic/polymer composite is one of the most common composite types used for sensing applications, owing to their tailorable properties of the two-phase composition, consisting of a three-dimensionally connected polymer/rubber matrix (inactive phase) with a dispersion of isolated piezo-ceramic particles (active phase). This thesis describes a method to develop novel biocompatible perivascular band comprised of a two-phase piezo-active composite to be fabricated using simple manufacturing processes. Biomaterials such as tissue scaffolds comprised of silk fibroin (SF) and chitosan (CS), and biocompatible soft rubbers will be implemented ...


3d Printing Of Biodegradable Scaffolds For Tissue Engineering Applications, Joe Morin, Michael Pickett, Amy Abraham, Tiera Martinelli 2017 Cedarville University

3d Printing Of Biodegradable Scaffolds For Tissue Engineering Applications, Joe Morin, Michael Pickett, Amy Abraham, Tiera Martinelli

The Research and Scholarship Symposium

With the recent improvements in three dimensional (3D) printing technologies, the potential for tissue engineering and regenerative medicine have significantly improved. One key idea in tissue engineering is to specifically design scaffolds to aid in the healing process by being incorporated into the body’s own tissue. The overall goal of this project is to investigate 3D printable scaffold design to access suitability for tissue replacement. This was accomplished by analyzing the effect of the material used to create the scaffolds, pore size, and pore shape on mechanical stiffness and cell culturability. Based on published literature, it was determined that ...


Chemical And Physical Priming Of Human Mesenchymal Stem Cells To Alter Nonviral Gene Delivery Outcomes, Tyler Kozisek, Andrew Hamann, Amy Mantz, Mathias Schubert, Eva Schubert, Angela K. Pannier 2017 University of Nebraska-Lincoln

Chemical And Physical Priming Of Human Mesenchymal Stem Cells To Alter Nonviral Gene Delivery Outcomes, Tyler Kozisek, Andrew Hamann, Amy Mantz, Mathias Schubert, Eva Schubert, Angela K. Pannier

Biological Systems Engineering--Dissertations, Theses, and Student Research

Human Mesenchymal Stem Cells: Human mesenchymal stem cells (hMSCs) are a multipotent cell, meaning they are able to differentiate into a more mature cell type, such as osteocytes, chondrocytes, and adipocytes, that are found in numerous tissues in the human body, such as bone marrow, fat, and muscle. Since hMSCs can be derived from adult human tissues, they do not have the same ethical concern associated with them as other stem cells, such as embryonic stem cells. Due to hMSCs multipotency and ease of obtaining, they have become one of the most widely researched stem cell types in areas such ...


Computing Spatiotemporal Heat Maps Of Lipid Electropore Formation: A Statistical Approach, Willy Wriggers, Frederica Castellani, Julio A. Kovacs, P. Thomas Vernier 2017 Old Dominion University

Computing Spatiotemporal Heat Maps Of Lipid Electropore Formation: A Statistical Approach, Willy Wriggers, Frederica Castellani, Julio A. Kovacs, P. Thomas Vernier

Mechanical & Aerospace Engineering Faculty Publications

We extend the multiscale spatiotemporal heat map strategies originally developed for interpreting molecular dynamics simulations of well-structured proteins to liquids such as lipid bilayers and solvents. Our analysis informs the experimental and theoretical investigation of electroporation, that is, the externally imposed breaching of the cell membrane under the influence of an electric field of sufficient magnitude. To understand the nanoscale architecture of electroporation, we transform time domain data of the coarse-grained interaction networks of lipids and solvents into spatial heat maps of the most relevant constituent molecules. The application takes advantage of our earlier graph-based activity functions by accounting for ...


Shape Recovery With Concomitant Mechanical Strengthening Of Amphiphilic Shape Memory Polymers In Warm Water, Ben Zhang, Janae E. DeBartolo, Jie Song 2017 University of Massachusetts Medical School

Shape Recovery With Concomitant Mechanical Strengthening Of Amphiphilic Shape Memory Polymers In Warm Water, Ben Zhang, Janae E. Debartolo, Jie Song

University of Massachusetts Medical School Faculty Publications

Maintaining adequate or enhancing mechanical properties of shape memory polymers (SMPs) after shape recovery in an aqueous environment are greatly desired for biomedical applications of SMPs as self-fitting tissue scaffolds or minimally invasive surgical implants. Here we report stable temporary shape fixing and facile shape recovery of biodegradable triblock amphiphilic SMPs containing a poly(ethylene glycol) (PEG) center block and flanking poly(lactic acid) or poly(lactic-co-glycolic acid) blocks in warm water, accompanied by concomitant enhanced mechanical strengths. Differential scanning calorimetry (DSC), wide-angle X-ray diffraction (WXRD), and small-angle X-ray scattering (SAXS) analyses revealed that the unique stiffening of the amphiphilic ...


Differentiation Of Kidney Progenitors Using Induced Pluripotent Stem Cells And Conditioned Media Of Renal Cortical Tubular Epithelial Cells, Matthew Nielsen 3644978, Steven Passey, Maxwell Beers, Alonzo Cook 2017 Brigham Young University - Provo

Differentiation Of Kidney Progenitors Using Induced Pluripotent Stem Cells And Conditioned Media Of Renal Cortical Tubular Epithelial Cells, Matthew Nielsen 3644978, Steven Passey, Maxwell Beers, Alonzo Cook

Biomedical Engineering Western Regional Conference

No abstract provided.


Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D. 2017 Brigham Young University - Provo

Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D.

Biomedical Engineering Western Regional Conference

Currently, the only permanent cure for Type 1 Diabetes is a pancreatic or islet transplant. With the shortage of donors, we are progressing research towards alternative therapies by differentiating induced pluripotent stem cells into derived pancreatic β-cells that can be transplanted and used as treatment for Type 1 Diabetes. We are investigating methods to increase the yield of fully derived, insulin producing pancreatic β-cells and are additionally researching the use of hydrophilized expanded polytetrafluoroethylene for use in cell containment devices. These devices could offer a future alternative for islet transplantation in human patients.


Beating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes On Porcine Extracellular Matrix, Kaitlyn J. McEntire, Alonzo Cook, Toph Knutson, Gevan Eldredge, Hunter Behrmann, Clayton Holding, Matthew Hodgson, Dillon Despain, Jacob Preslar, Joseph Rich, Matthew Stephens, Matthew Trone, Michael Neff, Sam Worrall, Joshua McClellan, Abbie Kondel, Donnie Pfeifer 2017 Brigham Young University - Provo

Beating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes On Porcine Extracellular Matrix, Kaitlyn J. Mcentire, Alonzo Cook, Toph Knutson, Gevan Eldredge, Hunter Behrmann, Clayton Holding, Matthew Hodgson, Dillon Despain, Jacob Preslar, Joseph Rich, Matthew Stephens, Matthew Trone, Michael Neff, Sam Worrall, Joshua Mcclellan, Abbie Kondel, Donnie Pfeifer

Biomedical Engineering Western Regional Conference

Despite modern technology and developments in heart disease treatment and prevention, heart disease remains the number one cause of death in America. With an inability to meet an ever-increasing demand for heart transplants and the dangers of immunosuppressant drugs, any potential alternative to cardiac transplantation must be pursued. The end goal of this research is to engineer biocompatible tissues that are fully functional to repair or replace damaged portions of the heart following the loss of cardiac function. This research investigates the effect of porcine extracellular matrix as the scaffold for beating IPS-differentiated cardiomyocytes.


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