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Molecular, Cellular, and Tissue Engineering Commons

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All Articles in Molecular, Cellular, and Tissue Engineering

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Characterization Of Molecular Communication Based On Cell Metabolism Through Mutual Information And Flux Balance Analysis, Zahmeeth Sayed Sakkaff 2016 University of Nebraska - Lincoln

Characterization Of Molecular Communication Based On Cell Metabolism Through Mutual Information And Flux Balance Analysis, Zahmeeth Sayed Sakkaff

Computer Science and Engineering: Theses, Dissertations, and Student Research

Synthetic biology is providing novel tools to engineer cells and access the basis of their molecular information processing, including their communication channels based on chemical reactions and molecule exchange. Molecular communication is a discipline in communication engineering that studies these types of communications and ways to exploit them for novel purposes, such as the development of ubiquitous and heterogeneous communication networks to interconnect biological cells with nano and biotechnology-enabled devices, i.e., the Internet of Bio-Nano Things. One major problem in realizing these goals stands in the development of reliable techniques to control the engineered cells and their behavior from ...


A Multi-Channel 3d-Printed Bioreactor For Evaluation Of Growth And Production In The Microalga Dunaliella Sp, Cristian A. Cox Mr 2016 University of Maine

A Multi-Channel 3d-Printed Bioreactor For Evaluation Of Growth And Production In The Microalga Dunaliella Sp, Cristian A. Cox Mr

Electronic Theses and Dissertations

We explored the capabilities of additive manufacturing using a photo-cured jetted material 3D printer to manufacture a milli-microfluidic device with direct application in microalgae Dunaliella sp growth and intracellular compounds biosynthesis tests. A continuous microbioreactor for microalgae culture was CAD designed and successfully built in 1 hour and 49 minutes using black photopolymer cured by UV and a support material. The microreactor was made up of 2 parts including the bioreactor itself and a microchannel network for culture media fluids and microalgae. Both parts were assembled to form a single unit. Additional optical and auxiliar components were added. An external ...


Electrospinning Of Poly (Ester Amide) Fibres For Mesenchymal Progenitor Cell Differentiation, Sarah Kiros 2016 The University of Western Ontario

Electrospinning Of Poly (Ester Amide) Fibres For Mesenchymal Progenitor Cell Differentiation, Sarah Kiros

Electronic Thesis and Dissertation Repository

The in vitro vascular tissue engineering paradigm seeks to produce biologically responsive vascular substitutes using cells, biodegradable scaffolds, and bioreactors to mature the tissue for the potential treatment of vascular occlusions and to create 3D tissue models for pre-clinical testing. In this work, a poly (ester amide) (PEA) derived from from L-phenylalanine, sebacoyl chloride and 1,4 butanediol was synthesized and electrospun to form both 3D fibrous mats and tubular constructs. Both the polymer solution concentration and mandrel rotation speed were optimized to fabricate bead-free fibres. Cytocompatibility and proliferation studies using mesenchymal progenitor 10T1/2 cells showed PEA fibres were ...


Surface Engineering Of Titanium Alloy To Stimulate The Bone Regeneration, Yubin Meng, Zhenduo Cui, Zhaoyang Li, Xue Li, Jin Zhao, Xubo Yuan, Xianjin Yang 2016 Tianjin University

Surface Engineering Of Titanium Alloy To Stimulate The Bone Regeneration, Yubin Meng, Zhenduo Cui, Zhaoyang Li, Xue Li, Jin Zhao, Xubo Yuan, Xianjin Yang

The 8th International Conference on Physical and Numerical Simulation of Materials Processing

No abstract provided.


Stem Cell Based Tissue Engineering And Regenerative Medicine: A Review Focusing On Adult Stem Cells, Jordan Greenberg, Veronica R. Fortino, Daniel Pelaez, Herman S. Cheung 2016 University of Miami

Stem Cell Based Tissue Engineering And Regenerative Medicine: A Review Focusing On Adult Stem Cells, Jordan Greenberg, Veronica R. Fortino, Daniel Pelaez, Herman S. Cheung

Veronica Fortino

Tissue engineering has emerged as a field that attempts to harness the bodies' own developmental and repair features to treat diseases and illnesses. Many of these illnesses are caused by necrosis or loss of functionality of complete organs or specific cell types. Early discoveries in embryonic stem cells fueled a wave of research that led to claims about possibly regenerating nonfunctioning organs. Although we are still far away from being able to grow functional organs in a Petri dish, the field continues to progress forward, and new clinical trials have been approved for using both embryonic and adult stem cell ...


Adipose-Derived Stem Cell Differentiation In Cell Aggregates Supplemented With Micronized, Decellularized Extracellular Matrix, Danielle Heinbuch 2016 The University of Western Ontario

Adipose-Derived Stem Cell Differentiation In Cell Aggregates Supplemented With Micronized, Decellularized Extracellular Matrix, Danielle Heinbuch

Electronic Thesis and Dissertation Repository

In the human body there are tissues with limited regenerative potential, raising the need for cell-based regenerative therapies. The main objective of this thesis was to examine how tissue-specific extracellular matrix (ECM) affects the differentiation of human adipose-derived stem/stromal cells (ASCs). The effects of incorporating milled, decellularized adipose (DAT) or cartilage (DCT) ECM particles on ASC lineage-specific differentiation in 3-D cell aggregate cultures were explored. The results demonstrated that the addition of ECM improved differentiation for both adipogenesis and chondrogenesis. Analysis of adipogenic gene and protein expression indicated enhanced differentiation in the DAT+ASC aggregates relative to the DCT ...


Characterization Of Histological Changes In The Microvasculature Of Rat Skeletal Muscle After Spinal Cord Injury, Sally Lin 2016 Marquette University

Characterization Of Histological Changes In The Microvasculature Of Rat Skeletal Muscle After Spinal Cord Injury, Sally Lin

Master's Theses (2009 -)

The purpose of this study was to determine whether there are histological changes in the microvasculature of rat skeletal muscle following chronic spinal cord injury both above and below the level of injury. This study is important because microvascular structure likely impacts muscle performance and cardiovascular health. To the best of our knowledge, this is the only study to investigate microvascular structure within rat skeletal muscle after spinal cord injury. We hypothesized structural remodeling would occur in both the myofibers and microvasculature, which would then manifest in differences in myofiber cross sectional area and microvascular diameter, wall thickness, wall to ...


Bone Tissue Engineering: Scalability And Optimization Of Densified Collagen-Fibril Bone Graft Substitute Materials, John G. Nicholas, Lauren E. Watkins, Sherry L. Voytik-Harbin 2016 Purdue University

Bone Tissue Engineering: Scalability And Optimization Of Densified Collagen-Fibril Bone Graft Substitute Materials, John G. Nicholas, Lauren E. Watkins, Sherry L. Voytik-Harbin

The Summer Undergraduate Research Fellowship (SURF) Symposium

Over 240 million people missing teeth worldwide experience lingering problems such as difficulty speaking and eating, undesirable aesthetics, and resorption of bone supporting neighboring teeth. The gold standard of treatment utilizes grafts to attach a function-restoring implant to supporting bone. Current graft materials suffer from problems including autologous donor site morbidity, long resorption time, incomplete integration with the maxillae or mandible, and structural weakness. Patient-specific, cellularized bone grafts may be a solution to these issues by accelerating and improving the quality of regenerated bone. Recently, encapsulation of mesenchymal stem cells within self-assembling type I collagen oligomer matrices has been shown ...


Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, Evan Surma, Sherry L. Harbin, Hongji Zhang, Stacy Halum 2016 Purdue University

Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, Evan Surma, Sherry L. Harbin, Hongji Zhang, Stacy Halum

The Summer Undergraduate Research Fellowship (SURF) Symposium

Lower back pain from intervertebral disc injury affects around 84% of the population at some point in their life, which at its worst may cause total immobilization. This pain can only be temporarily relieved by spinal fusion or intervertebral disc replacement; however, both of these cause loss of natural motion in patients by removing damaged fibrocartilage discs. While these techniques help mitigate pain briefly, no permanent solution exists currently to both relieve pain and preserve natural motion. My work may be a solution by eventually providing patient-specific implants that resemble native tissue in the regeneration process that could be absorbed ...


Lysis And Amplifciation Of Neonatal Sepsis Causing Pathogens, Gregory Berglund, Elizabeth A. Phillips, Jacqueline C. Linnes 2016 Purdue University

Lysis And Amplifciation Of Neonatal Sepsis Causing Pathogens, Gregory Berglund, Elizabeth A. Phillips, Jacqueline C. Linnes

The Summer Undergraduate Research Fellowship (SURF) Symposium

Neonatal sepsis, resulting from a bloodstream infection within the first few weeks of life, is the leading cause of newborn deaths worldwide. The gold standard of neonatal sepsis diagnosis requires a blood culture to identify the infecting bacteria, however require days of incubation, expensive equipment, and expertise. Any delay in diagnosis is critical, as the condition can be treated easily if appropriate antibiotics are administered promptly. A low-cost, rapid, and sensitive diagnostic test would enable more timely treatment and lead to better patient outcomes with fewer required resources. Point-of-care, nucleic acid amplification assays are a promising alternative to blood culture ...


Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song 2016 University of Massachusetts Medical School

Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song

Jie Song

Biodegradable polymers have been widely utilized as drug delivery vehicles and tissue engineering scaffolds. We previously designed amphiphilic triblock copolymer poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) (PELA) and its hydroxyapatite (HA) composites for bone tissue engineering applications. The hydrophilic electrospun PELA-HA composite exhibited aqueous stability and elastic handling characteristics, and was able to template the proliferation and osteogenesis of bone marrow stromal cells (BMSCs) in vitro and in vivo when spiral-wrapped into cylinders and press-fit into critical size femoral segmental defects in rats. However, the slow degradation of PELA has prevented timely disappearance of the scaffold and ...


Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin 2016 University of Nebraska - Lincoln

Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin

Biological Systems Engineering--Dissertations, Theses, and Student Research

The establishment of in vitro culture models utilizes tissue engineering principles to design functional mimics of in vivo environments in vitro. Advantages for the use of in vitro culture models include ethical alleviation of animal models for therapeutic testing, cost efficiency, and a greater ability to study specific mechanisms via a systematic, ground-up approach to development. In this thesis, alginate hydrogels are utilized in the development of in vitro culture models of porcine embryo elongation and growth plate cartilage development. First, the effect of scaffold and modifications to the scaffold were explored in both projects. In order to modulate cell-scaffold ...


Automated Microscopy Platform For High-Throughput Analysis Of Cellular Characteristics, Hussam Ibrahim 2016 Augustana College - Rock Island

Automated Microscopy Platform For High-Throughput Analysis Of Cellular Characteristics, Hussam Ibrahim

Physics: Student Scholarship & Creative Works

Existing microscopy platforms allow analysis post-hoc, but not in real time. This is an issue in the world of Bioengineering because you are limited to performing further analysis on specimen. The aim of my research was to design a sophisticated system whereby information can be exchanged between the software which acquires images and software that analyzes the images immediately after acquisition. In this system, images would be acquired by the microscope and analyzed by customized scripts (MATLAB, Mathworks) in real time. Specifically, MATLAB would wait for new images to be saved on the hard drive, import these images, and perform ...


Calcium Phosphate As A Key Material For Socially Responsible Tissue Engineering, Vuk Uskoković, Victoria M. Wu 2016 Chapman University

Calcium Phosphate As A Key Material For Socially Responsible Tissue Engineering, Vuk Uskoković, Victoria M. Wu

Pharmacy Faculty Articles and Research

Socially responsible technologies are designed while taking into consideration the socioeconomic, geopolitical and environmental limitations of regions in which they will be implemented. In the medical context, this involves making therapeutic platforms more accessible and affordable to patients in poor regions of the world wherein a given disease is endemic. This often necessitates going against the reigning trend of making therapeutic nanoparticles ever more structurally complex and expensive. However, studies aimed at simplifying materials and formulations while maintaining the functionality and therapeutic response of their more complex counterparts seldom provoke a significant interest in the scientific community. In this review ...


Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song 2016 University of Massachusetts Medical School

Optimization Of The Design Of An Amphiphilic Biodegradable Polymer For Tissue-Engineering Application, Ben Zhang, Tera M. Filion, Jie Song

UMass Center for Clinical and Translational Science Research Retreat

Biodegradable polymers have been widely utilized as drug delivery vehicles and tissue engineering scaffolds. We previously designed amphiphilic triblock copolymer poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) (PELA) and its hydroxyapatite (HA) composites for bone tissue engineering applications. The hydrophilic electrospun PELA-HA composite exhibited aqueous stability and elastic handling characteristics, and was able to template the proliferation and osteogenesis of bone marrow stromal cells (BMSCs) in vitro and in vivo when spiral-wrapped into cylinders and press-fit into critical size femoral segmental defects in rats. However, the slow degradation of PELA has prevented timely disappearance of the scaffold and ...


Implantable Microenvironments To Capture Stable-To- Aggressive Tumor Transition, Ryan Carpenter, Jungwoo Lee 2016 University of Massachusetts Amherst

Implantable Microenvironments To Capture Stable-To- Aggressive Tumor Transition, Ryan Carpenter, Jungwoo Lee

UMass Center for Clinical and Translational Science Research Retreat

Clinical stability occurs when cancers reach a state where the disease neither advances nor regresses. Tumors can remain in this state for multiple years before progressing to more aggressive phenotypes. The mechanisms for maintaining a stable state and the factors that contribute to tumor activation are poorly understood. We hypothesized that an implantable biomaterial scaffold would be able to isolate a population of stable tumor cells that could then be used to study the transition to an aggressive phenotype. In this work we developed a tunable and highly controlled, porous acrylamide scaffold and subcutaneously implanted them in immunodeficient (NSG) mice ...


Lipoaspirate And Adipose Stem Cells As Potential Therapeutics For Chronic Scars, Dylan Perry, Jorge Lujan-Hernandez, Michael S. Chin, So-Yun Min, Ava Chappell, Raziel Rojas-Rodriguez, Raghu Appasani, Patrick Teebagy, Silvia Corvera, Janice F. Lalikos 2016 University of Massachusetts Medical School

Lipoaspirate And Adipose Stem Cells As Potential Therapeutics For Chronic Scars, Dylan Perry, Jorge Lujan-Hernandez, Michael S. Chin, So-Yun Min, Ava Chappell, Raziel Rojas-Rodriguez, Raghu Appasani, Patrick Teebagy, Silvia Corvera, Janice F. Lalikos

UMass Center for Clinical and Translational Science Research Retreat

Introduction: Burn injuries can lead to hypertrophic or keloid scars, causing pain and long lasting mobility issues. Current therapies are often unsatisfactory, costly, or morbid. Prior studies suggest adipose derived stem cells (ADSCs) and lipoaspirate can improve scar outcomes of acute thermal wounds. Clinical reports suggest lipoaspirate and ADSCs can improve chronic burn scar remodeling. However, this has not been extensively studied in animal models. We sought to determine if adipose tissue can improve chronic scar remodeling and to compare the effects of ADSCs and processed lipoaspirate.

Methods: 50 CD1 nu/nu athymic mice received a standardized deep partial-thickness thermal ...


Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez 2016 Augustana College, Rock Island Illinois

Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez

Biology: Student Scholarship & Creative Works

ABSTRACT: The HIV-1 pandemic continues to thrive due to ineffective HIV-1 vaccines. Historically, the world’s most infectious diseases, such as polio and smallpox, have been eradicated or have come close to eradication due to the advent of effective vaccines. Highly active antiretroviral therapy is able to delay the onset of AIDS but can neither rid the body of HIV-1 proviral DNA nor prevent further transmission. A prophylactic vaccine that prevents the various mechanisms HIV-1 has to evade and attack our immune system is needed to end the HIV-1 pandemic. Recent advances in engineered nuclease systems, like the CRISPR/Cas9 ...


Inter-Droplet Membranes For Mechanical Sensing Applications, Nima Tamaddoni Jahromi 2016 University of Tennessee - Knoxville

Inter-Droplet Membranes For Mechanical Sensing Applications, Nima Tamaddoni Jahromi

Doctoral Dissertations

This dissertation combines self-assembly phenomena of amphiphilic molecules with soft materials to create and characterize mechanoelectrical transducers and sensors whose sensing elements are thin-film bioinspired membranes comprised of phospholipids or amphiphilic polymers. We show that the structures of these amphiphilic molecules tune the mechanical and electrical properties of these membranes. We show that these properties affect the mechanoelectrical sensing characteristic and range of operation of these membrane transducers. In the experiments, we construct and characterize a membrane-based hair cell embodiment that enables the membrane to be responsive to mechanical perturbations of the hair. The resulting oscillations of membranes formed between ...


Interaction Of Fibrinogen With Fibronectin: Purification And Characterization Of A Room Temperature-Stable Fibrinogen-Fibronectin Complex From Normal Human Plasma, Ayman E. Ismail 2016 University of Nebraska-Lincoln

Interaction Of Fibrinogen With Fibronectin: Purification And Characterization Of A Room Temperature-Stable Fibrinogen-Fibronectin Complex From Normal Human Plasma, Ayman E. Ismail

Chemical & Biomolecular Engineering Theses, Dissertations, & Student Research

A fibrinogen-fibronectin complex (γγ’pdFI-pdFN) was purified from normal human plasma using a sequence of cryoprecipitation, ammonium sulfate fractionation, and DEAE Sepharose chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing condition showed both a 1:1 stoichiometric ratio of fibrinogen (FI) to fibronectin (FN) as well as a stoichiometric ratio of 1:1 of γg to gγ’. The γγ’pdFI-pdFN complex was non-covalent in nature as it was disrupted by affinity adsorption to Gelatin Sepharose where pdFN bound strongly and the disrupted γγ’pdFI fell through the chromatographic column. Surprisingly, the purified γγ’pdFI-pdFN complex was more broadly ...


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