Plant Breeding and Genetics Commons^™

Hormon Etilen Dan Auksin Serta Kaitannya Dalam Pembentukan Tomat Tahan Simpan Dan Tanpa Biji, Syariful Mubarok, Alin Robiah Al Adawiyah, Arin Rosmala, Fathi Rufaidah, Anne Nuraini, Erni Suminar

Jurnal Kultivasi

Tomat merupakan salah satu komoditas tanaman hortikultura penting di Indonesia. Seiring dengan perkembangan teknologi, sudah banyak dikembangkan varietas tomat baru yang mempunyai karakter-karakter yang diinginkan antara lain tahan akan penyakit, produktivitas tinggi tinggi, kandungan nutrisi tinggi dan lain-lain. Penerapan teknologi melalui pendekatan hormonal sangat penting untuk menghasilkan jenis tomat baru, diantaranya adalah hormone etilen dan auksin. Kedua hormone tersebut sangat berpengaruh terhadap pertumbuhan dan hasil tomat khususnya untuk menghasilkan tomat yang adaptif pada kondisi lingkungan tercekam dan ketahanan simpan buah yang tinggi. Teknologi mutasi pada kedua hormone tersebut sudah dapat menghasilkan tomat yang diinginkan diantaranya adalah tomat iaa9-3 yang merupakan …

The Virome Of Peony And The Population Structure Of Its Most Prominent Viruses, Cullen Shaffer

Graduate Theses and Dissertations

Peony (Peonia lactiflora, Pall.) is a popular ornamental that has been cultivated for millennia. Due to its popularity, plant material is frequently moved across international borders allowing for the spread of viruses. The virome of several peony plants was investigated and four viruses; namely Amazon lily mild mottle virus (ALiMMV), Cycas necrotic stunt virus (CNSV), Gentian Kobu-sho associated virus (GKaV) and Lychnis mottle virus (LycMoV) were detected for the first time in the Western Hemisphere. Incidence ranged from a few plants for ALiMMV to near universal infection for CNSV. GKaV was found in individuals that were infected with Lemoine’s disease …

Selection And Propagation Of Pinyon Pine, Kylie M. Lawson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Single-leaf pinyon pines are drought tolerant trees native to the Great Basin. This species is a source of wild-collected, edible pine nuts that are in great demand. With no previous research apparent, this thesis aimed to identify wild trees with high cone production as sources for evaluating grafting onto immature and mature trees. Wild sources of scions were identified from four wild stands and six trees per stand. Counting and analyzing the number of scars left by mature cones along the leader branch provided an estimate for each tree‚Äôs productivity and identified trees with greater productivity within a stand. The …

Fine-Scale Patterns Of Genetic Structure In The Host Plant Chamaecrista Fasciculata (Fabaceae) And Its Nodulating Rhizobia Symbionts, Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa A. Wallace

Biological Sciences Faculty Publications

In natural plant populations, a fine-scale spatial genetic structure (SGS) can result from limited gene flow, selection pressures or spatial autocorrelation. However, limited gene flow is considered the predominant determinant in the establishment of SGS. With limited dispersal ability of bacterial cells in soil and host influence on their variety and abundance, spatial autocorrelation of bacterial communities associated with plants is expected. For this study, we collected genetic data from legume host plants, Chamaecrista fasciculata, their Bradyrhizobium symbionts and rhizosphere free-living bacteria at a small spatial scale to evaluate the extent to which symbiotic partners will have similar SGS …

Characterization Of Genetic Sources Associated With Restorability And Seed Dimension In Arkansas Restorer Rice Lines, Ozgur Azapoglu

Graduate Theses and Dissertations

Hybrid rice (Oryza sativa L.) breeding offers a significant opportunity to enhance rice production, and the cultivation of a male sterile line is the most important factor in the success of cross-breeding. One of the key elements of hybrid rice production is to develop a restorer line that is assigned as the male parent. The restorer lines provide viable pollen for fertilization of the male sterile plant due to the presence of a restorer gene (Rf) in their genomes. Any superior restorer line applied to hybrid rice production must contain genes/quantitative trait loci (QTL) associated with the desirable agronomic traits …

Estimation Of Additive And Dominance Effects Of A Mutant Glutathione S-Transferase Gene On Anthocyanin And Proanthocyanidin Content In Muscadine Grape (Vitis Rotundifolia), Autumn Brown

Horticulture Undergraduate Honors Theses

The skin color of muscadine grapes (Vitis rotundifolia) is typically classified as black or bronze. A glutathione S-transferase, VrunGST4, has been identified as a candidate gene for berry skin color in muscadine grapes. A molecular marker was developed within VrunGST4 to distinguish between muscadine genotypes (cultivars and selections) with bronze (T:T), heterozygote black (C:T), and homozygote black (C:C) berries. The objectives of this study were to determine whether there was a correlation between berry skin color and total anthocyanin content and to calculate additive and dominance effects of VrunGST4 in determining total anthocyanins in the berries and …

Improving Breeding Program Efficiency And Genetic Gain Through The Implementation Of Genomic Selection In Diverse Wheat Germplasm, Dylan Larkin

Graduate Theses and Dissertations

Genomic selection (GS) is an important tool for increasing genetic gain for economically important traits in breeding programs. Genomic selection uses molecular markers across the entire genome in order to predict the performance of breeding lines for a trait of interest prior to phenotyping. A training population (TP) of elite germplasm, representative of the University of Arkansas wheat breeding program, was developed in order to predict important agronomic and Fusarium head blight (FHB) resistance traits within the University of Arkansas wheat breeding program through cross-validation and forward prediction.

A genome-wide association study (GWAS) was performed on the TP to identify …

Resistance To Herbicides Conferred By Amaranthus Palmeri Protoporphyrinogen Ix Oxidase Mutations, Pamela Carvalho De Lima

Graduate Theses and Dissertations

Palmer amaranth (Amaranthus palmeri S. Wats.) is one of the most problematic agronomic weeds to control in fields across Arkansas. Thus far, this species has evolved resistance to several herbicides, including protoporphyrinogen IX oxidase (PPO) inhibitors. The majority of PPO-resistant Palmer amaranth populations harbor a target-site mutation (substitution or deletion of amino acids). The objective of this thesis was to identify the level of fomesafen resistance conferred by PPO2 mutations from Palmer amaranth. The experiments conducted aimed to (1) characterize the level of resistance conferred by the transgene Palmer amaranth ppo2 carrying ΔG210 mutation into the wild type rice (Oryza …

Molecular Genetic Analysis Of Drought Resistance And Productivity Traits Of Rice Genotypes, Yheni Dwiningsih

Graduate Theses and Dissertations

Rice (Oryza sativa L.) is the staple food for a majority of the world’s population, and uses 30% of the global fresh water during its life cycle. Drought at the reproductive stage is the most important abiotic stress factor limiting grain yield. The United States is the third largest exporter of rice, and Arkansas is the top rice-producing state. The Arkansas rice-growing region in the Lower Mississippi belt is among the 10 areas with the highest risk of water scarcity. Adapted U.S. rice cultivars were screened for drought resistant (DR) traits to find sources for breeding U.S. rice cultivars for …

Association Mapping For Soybean (Glycine Max L. Merr.) Protein And Oil Content, Joseph Najjar

Graduate Theses and Dissertations

Soybean [Glycine Max (L.) Merrill] is an important economic crop because of its high content of seed protein and oil. Seed oil and protein content are quantitative inherited traits. The genetics of seed protein and oil levels have been extensively studied, with 367 QTL reported for protein and 475 QTL reported for oil to date. Validation of such QTLs, and identification of easily-automatable molecular markers around these QTL will aid the progression of breeding for such traits. The focus of this research was to discover novel and verify previously reported QTL related to protein and/or oil content via Genome-Wide Association …

Dct4—A New Member Of The Dicarboxylate Transporter Family In C4 Grasse, Sarit Weissmann, Pu Huang, Madeline A. Wiechert, Koki Furuyama, Thomas P. Brutnell, Mitsutaka Taniguchi, James C. Schnable, Todd C. Mockler

Center for Plant Science Innovation: Faculty Publications

Malate transport shuttles atmospheric carbon into the Calvin–Benson cycle during NADP-ME C4 photosynthesis. Previous characterizations of several plant dicarboxylate transporters (DCT) showed that they efficiently exchange malate across membranes. Here, we identify and characterize a previously unknown member of the DCT family, DCT4, in Sorghum bicolor. We show that SbDCT4 exchangesmalateacrossmembranesanditsexpressionpatternisconsistentwitharoleinmalatetransportduringC4 photosynthesis. SbDCT4 is not syntenic to the characterized photosynthetic gene ZmDCT2, and an ortholog is not detectable in the maize reference genome. We found that the expression patterns of DCT family genes in the leaves of Zea mays, and S. bicolor varied by cell type. Our results suggest that …

The Benefits Of The Arkansas Rice Check-Off Program, B. Peterson-Wilhelm, L. L. Nalley, A. Durand-Morat, A. Shew, R. Parajuli, G. Thoma

AAES Research Reports and Research Bulletins

As margins are reducing for agricultural producers there is a concerted effort to analyze all costs. One such cost for rice producers in Arkansas is their contribution to the Rice Check-off Program. This study analyzes the cost-benefit ratio of funds contributed by Arkansas rice producers and the holistic (both economic and environmental) benefits they receive. This study analyzes just five of the many programs the Rice Check-off Program invests in through the University of Arkansas System Division of Agriculture (UASDA) and suggests that every dollar invested generated an average return of $28.49 between 2002–2018 ($70.45 when ecosystem benefits are included). …

Hu Aquaponics Monitoring And Control System : European Annual Edunet Conference 2020, Rachel L. Fogle, Glenn P. Williams, Josh R. Krug

Harrisburg University Presidential Research Grants

The functional purpose of the HU Aquaponics Monitoring and Control System Project is to develop an environmental and plant monitoring and control system for the HU Aquaponics Lab, located in the Student Union. The project involves the design and implementation of technology that will regularly take measurements from the environment (e.g., air temperature, water temperature, pH, dissolved oxygen, etc). PLCnext Technology will systematically collect, store, and web-publish the measurement data for HU researchers and the public to use for scientific research.

Arkansas Wheat Cultivar Performance Tests 2019-2020, R. E. Mason, R. G. Miller, D. E. Moon, J. P. Kelley, J. Carlin

Arkansas Agricultural Experiment Station Research Series

Wheat cultivar performance tests are conducted each year in Ark- ansas by the University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station, Department of Crop, Soil, and Environmental Sciences. The tests provide informa- tion to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating cultivar recommendations for small-grain producers. The tests are conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Newport Extension Center near Newport, the Rohwer Research Station near Rohwer, the Pine …

Summaries Of Arkansas Cotton Research 2019, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

Statewide, temperatures and precipitation were mostly above average (https://www.weather.gov/lzk/cli2019atxt.htm). The 2019 season was the wettest since 2015, and the 7th wettest on record. The wettest month was May, and the driest month was September. By average temperature, the coldest month was January, and the warmest month was August. Considering departures from normal, the most significant warmth occurred in September, which was 7.0 degrees above average. Many fields were muddy or underwater, given a lot of rain early in the year, and extensive river flooding (including the historic Arkansas River flood). It was far too wet in some areas …

Evaluasi Galur-Galur Mutan Kedelai Berdasarkan Daya Hasil Dan Ukuran Biji Di Jatinangor, Sumedang, Jawa Barat, Andrew Yoel, Meddy Rachmadi

Jurnal Kultivasi

Usaha pemuliaan tanaman kedelai saat ini sebagian besar berfokus pada identifikasi genotipe dengan hasil biji yang tinggi dan ukuran biji besar. Tujuan dari penelitian ini adalah untuk mengevaluasi kultivar kedelai dan kedelai mutan untuk daya hasil tinggi dan ukuran biji besar. Penelitian ini dilaksanakan dari September 2017 sampai Januari 2018 di Kebun percobaan Ciparanje Fakultas Pertanian UNP AD, Jatinangor, Jawa Barat. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) yang terdiri dari 20 perlakuan dalam 2 ulangan. Perlakuan terdiri dari 19 genotipe dan 1 kultivar cek. Data dianalisis Sidik Ragam dengan Uji F 5%, nilai beda dengan Uji Dunnet …

Parameter Genetik Karakter Komponen Hasil Dan Seleksi 82 Genotipe Ercis Di Dataran Rendah, Mayang Ayudya Handini, Darmawan Saptadi, Budi Waluyo

Jurnal Kultivasi

Ercis (Pisum sativum L.) ialah salah satu tanaman dari famili Fabaceae dan tergolong tanaman legume yang memiliki nilai ekonomi cukup tinggi. Studi pemuliaan tanaman pada ercis ditujukan untuk mendapat hasil yang optimal di berbagai wilayah penanaman, salah satunya dataran rendah. Keberhasilan program seleksi memerlukan parameter genetik seperti keragaman genetik dan heritabilitas. Penelitian ini bertujuan untuk mengetahui keragaman genetik dan heritabilitas karakter 82 genotipe ercis dan menentukan genotipe yang berpotensi dikembangkan di dataran rendah. Percobaan dilaksanakan berdasarkan rancangan augmented design dengan 82 genotipe uji dan tiga pembanding (cek). Karakter yang memiliki keragaman luas dan heritabilitas tinggi terdapat pada karakter bobot brangkasan …

Respons Dua Mutan Tomat Terhadap Cekaman Kekeringan, Ayu Ratna Ningrum, Anne Nuraini, Erni Suminar, Syariful Mubarok

Jurnal Kultivasi

Kondisi cekaman kekeringan pada tanaman tomat dapat menyebabkan pertumbuhan dan produksi tanaman tomat menurun. Salah satu upaya yang dapat dilakukan untuk mengatasi masalah tersebut adalah dengan perakitan varietas tanaman baru yang tahan terhadap cekaman kekeringan. Beberapa hasil mutasi gen IAA pada tomat mutan Micro-Tom mampu menghasilkan tanaman yang toleran terhadap kondisi stress secara abiotik, yaitu pada galur iaa9-3 dan iaa9-5. Penelitian ini dilakukan untuk mengetahui respons pertumbuhan vegetatif pada iaa9-3 dan iaa9-5 dalam kondisi cekaman kekeringan dengan metode in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan dan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran pada bulan September sampai Desember 2019. Rancangan …

Penurunan Nilai Konduktansi Stomata, Efisiensi Penggunaan Cahaya, Dan Komponen Pertumbuhan Akibat Genangan Air Pada Beberapa Genotip Tanaman Tebu, Mochamad Arief Soleh, Intan Ratna Dewi Anjarsari, Santi Rosniawaty

Jurnal Kultivasi

Fenomena perubahan iklim mempengaruhi distribusi hujan yang tidak merata. Kelebihan air hujan di lahan akan menyebabkan genangan air karena sistem drainase lahannya kurang baik yang berdampak terganggunya pertumbuhan dan hasil tanaman tebu. Dalam kondisi tergenang (G), jumlah anakan beberapa genotip tebu menurun bila dibandingkan dengan kondisi tanpa genangan (TG) pada 77 hari setelah penggenangan (HSP), kecuali genotip tebu GMP1. Jumlah anakan tersebut berkisar antara 6,7 untuk genotip KK sampai 10,3 anakan untuk genotip GMP1. Sedangkan pada kondisi tanpa genangan kisaran jumlah adalah 8 untuk genotip PS864 sampai 13,7 untuk genotip KK. Kondisi genangan air juga telah menurunkan nilai konduktansi stomata …

Genetic Diversity: The Hub Of Plant Breeding, Foster Kangben

International Programs

Agriculture the world over is faced with the threat of food, fiber, and nutrition insecurity especially in the wake of climate change. However, employing two approaches to plant breeding, the molecular and phenotypic methods, can make use of genetic diversity in crop species in order to help surmount these challenges.

B.R. Wells Arkansas Rice Research Studies 2019, K.A. K. Moldenhauer, B. Scott, J. Hardke

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Taxonomic And Genetic Diversity, And Pathogenicity Of Diaporthe Species Associated With Soybean, Fakhir Eraheem Hameed Al Shuwaili

Graduate Theses and Dissertations

Diaporthe species (anamorph: Phomopsis) are associated with a wide range of plant hosts as plant pathogens, asymptomatic endophytes, and saprobes. One of these hosts is soybean, which is one of the most important crops in U.S. agriculture. Several Diaporthe species cause important diseases on soybean in the U.S., and specifically in Arkansas. The taxonomy, genetic diversity, and pathogenicity of Diaporthe species associated with asymptomatic infection of soybean are rarely studied with accurate molecular tools. Therefore, this dissertation aimed to assess the diversity and boundaries of Diaporthe associated with soybean in Arkansas. Furthermore, pathogenicity and alternative lifestyles were assessed among Diaporthe …

Arkansas Corn And Grain Sorghum Research Studies 2019, Victor Ford, Jason Kelley, Nathan Mckinney Ii

Arkansas Agricultural Experiment Station Research Series

The 2020 Arkansas Corn and Grain Sorghum Research Studies Series is the inaugural edition of this annual report and includes research results on all topics pertaining to corn and grain sorghum production including disease management, environmental/sustainability, irrigation, post-harvest drying, soil fertility, weed control, and research verification program results. Our objective is capturing and broadly distributing the results of research projects funded by the Arkansas Corn and Grain Sorghum Board. The intended audience includes producers and their advisors, current investigators, and future researchers. The Series will also serve as a citable archive of research results. Research reports contained in this publication …

Developing A High-Throughput Method To Screen Soybean Germplasm For Hypoxia Tolerance In Hydroponic Systems, Derrick Harrison

Graduate Theses and Dissertations

Soybean [Glycine max (Merr.)], the second most planted crop in the United States, is sensitive to field flooding due to depletion of oxygen and accumulation of CO2 in the rhizosphere. There is a need to breed cultivars that are adapted to areas prone to flooding, but field evaluations in the U.S. are limited because of time (one generation per year) and availability of resources (impermeable soils, irrigation, equipment to build levees). The purpose of this study was to develop and execute a protocol of germplasm screening for hypoxia tolerance using hydroponics in a controlled greenhouse environment. Germination rates and vigor …

Phenotypic And Genotypic Studies In The Peach [Prunus Persica (L.) Batsch] And Muscadine Grape (Vitis Rotundifolia Michx.), Jennifer Anne Lewter

Graduate Theses and Dissertations

Peach: Peaches [Prunus persica (L.) Batsch] are routinely chilled to increase shelf-life. Exposure to temperatures of 5° C for two weeks can induce chilling injury (CI) symptoms, including flesh mealiness (or wooliness) and a lack of juiciness. Phenotypic data were collected on seven biparental F1 peach populations maintained at the University of Arkansas Fruit Research Station. A genome wide association study (GWAS) was performed using TASSEL 5 which identified four quantitative trait loci (QTLs) associated with expressible juice, four QTLs for mealiness, five QTLs for soluble solids, and three QTLs for fruit weight. Exploiting these genetic markers could help breeders …

Genetics Of Physiological Traits Associated With Drought Tolerance In Soybean (Glycine Max), Sumandeep Kaur Bazzer

Graduate Theses and Dissertations

Soybean (Glycine max L.) is one of the major row crops in the United States, and its production is often limited by drought stress. Physiological traits from exotic germplasm that confer drought tolerance may be useful in improving commercial soybean production. For example, carbon isotope ratio (δ13C) is positively correlated with water use efficiency (WUE), and nitrogen isotope ratio (δ15N) is negatively correlated with N2 fixation; canopy temperature (CT) is an indicator for genetic variation in transpiration and stomatal conductance. Therefore, the objectives of this research were to identify the genomic regions associated with: (1) δ13C and δ15N using a …

Dynamic Transcriptomic And Phosphoproteomic Analysis During Cell Wall Stress In Aspergillus Nidulans, Cynthia Chelius, Walker Huso, Samantha Reese, Alexander Doan, Stephen Lincoln, Kelsi Lawson, Boa Tran, Raj Purohit, Trevor Glaros, Ranjan Srivastava, Steven D. Harris, Mark R. Marten

Center for Plant Science Innovation: Faculty Publications

The fungal cell-wall integrity signaling (CWIS) pathway regulates cellular response to environmental stress to enable wall repair and resumption of normal growth. This complex, interconnected, pathway has been only partially characterized in filamentous fungi. To better understand the dynamic cellular response to wall perturbation, a-glucan synthase inhibitor (micafungin) was added to a growing A. nidulans shake-flask culture. From this flask, transcriptomic and phosphoproteomic data were acquired over 10 and 120 min, respectively. To differentiate statistically-significant dynamic behavior from noise, a multivariate adaptive regression splines (MARS) model was applied to both data sets. Over 1800 genes were dynamically expressed and over …

Mapping Quantitative Trait Loci For Peach (Prunus Persica) Resistance To Xanthomonas Arboricola Pv. Pruni (Xap) And Determining The Diversity And Virulence Of A United States Xap Collection, Maxwell Vonkreuzhof

Graduate Theses and Dissertations

Bacterial spot, caused by the bacterium Xanthomonas arboricola pv. pruni (Xap), is a threat to the peach [Prunus persica (L.) Batsch], Japanese and European plum (P. salicina L. and P. domestica L.), and tart and sweet cherry (P. cerasus L. and P. avium L.) industries. Markers for fruit resistance to bacterial spot have been developed however, markers associated with foliar resistance have yet to be developed. A total of 130 progeny and 13 parents (n=143) were evaluated for foliar and fruit Xap resistance in 2013, 2014, and 2015, and 162 progeny and eight parents (n=170) in 2017 and 2018 as …

An Evaluation Of Unmanned Aircraft Systems' Ability To Assess Stripe Rust In Large Wheat Breeding Nursies, Jamison T. Murry

Graduate Theses and Dissertations

Stripe Rust (Puccinia striiformis f. sp. tritici) is a foliar disease that significantly impacts global wheat production, and resistant cultivars provide the most efficient method of control. High-throughput phenotyping using unmanned aircraft systems (UAS) offers a potentially more efficient method for field-based phenotyping compared to visual assessment. Here we tested the ability of remote sensing to predict stripe rust severity in a diverse population of 594 soft red winter wheat lines, planted in single-rows, and evaluated them by visually rating stripe rust intensity and remotely using the dark green color index (DGCI), normalized difference vegetation index (NDVI) and blue NDVI. …

Determination Of Critical Y-Leaf Potassium Concentrations Across Reproductive Growth Stages For Direct-Seeded Delay-Flooded Rice, Chandler Edwin Gruener

Graduate Theses and Dissertations

Rice (Oryza sativa L.) grain yield can be limited by potassium (K) deficiency on soils low in exchangeable K. Visually diagnosing K deficiency during early reproductive growth is not easily done and the interpretation of traditional tissue analysis is limited to select growth-stages. Our primary focus was to define continuous critical Y-leaf-K concentrations during reproductive growth for the production of maximal grain yield. A secondary objective was to examine Y-leaf sap-K concentration, measured using a handheld device, as a rapid in-field method of monitoring rice plant K nutrition. The Y-leaf is defined as the uppermost fully extended leaf with a …