Plant Breeding and Genetics Commons^™

Vitisnet: ‘‘Omics’’ Integration Through Grapevine Molecular Networks, Jerome Grimplet, Grant R. Cramer, Julie A. Dickerson, Kathy Mathiason, John Van Hemert, Anne Y. Fennell Dr

Agronomy, Horticulture and Plant Science Faculty Publications

Background: Genomic data release for the grapevine has increased exponentially in the last five years. The Vitis vinifera genome has been sequenced and Vitis EST, transcriptomic, proteomic, and metabolomic tools and data sets continue to be developed. The next critical challenge is to provide biological meaning to this tremendous amount of data by annotating genes and integrating them within their biological context. We have developed and validated a system of Grapevine Molecular Networks (VitisNet). Methodology/Principal Findings: The sequences from the Vitis vinifera (cv. Pinot Noir PN40024) genome sequencing project and ESTs from the Vitis genus have been paired and the …

Wheat (Triticum Aestivum) Nam Proteins Regulate The Translocation Of Iron, Zinc, And Nitrogen Compounds From Vegetative Tissues To Grain, Brian M. Waters, Cristobal Uauy, Jorge Dubcovsky, Michael A. Grusak

Department of Agronomy and Horticulture: Faculty Publications

The NAM-B1 gene is a NAC transcription factor that affects grain nutrient concentrations in wheat (Triticum aestivum). An RNAi line with reduced expression of NAM genes has lower grain protein, iron (Fe), and zinc (Zn) concentrations. To determine whether decreased remobilization, lower plant uptake, or decreased partitioning to grain are responsible for this phenotype, mineral dynamics were quantified in wheat tissues throughout grain development. Control and RNAi wheat were grown in potting mix and hydroponics. Mineral (Ca, Cu, Fe, K, Mg, Mn, P, S, and Zn) and nitrogen (N) contents of organs were determined at regular intervals to …

Evaluating Hazelnut Cultivars For Yield, Quality And Disease Resistance, Sam Tobin

Department of Environmental Studies: Undergraduate Student Theses

ABSTRACT This long term study focuses on testing various hazelnut cultivars for yield, nut quality and disease resistance. There are various cultivars that are being tested for these desired traits but only the Grand Traverse and Skinner will be applicable for the results of this localized study. The desired traits of commercial nut production are best matched by these two cultivars. Results from previous harvests will be used to draw trends to recommend commercially functional cultivars in Eastern Nebraska.

Crop Updates - 2009 Katanning, Murray Scholz, Peter Newman, Glenn Adam, Trevor Bell, Michael Whitehead, W. K. Anderson, Chris Gazey, J. Andrew, R. Pearce, Bill Bowden, Wayne Pluske, Jeremy Lemon, D. C. Edmeades, J. J. Russell, Blakely H. Paynter, David Ferris, Abul Hashen, Catherine Borger, Stephen B. Powels, Qin Yu, Mechelle Owen, Roberto Busi, Sudheesh Manalil, Sally Peltzer, David Minkey, Raj Malik

Crop Updates

This session covers seventeen papers from different authors

1. GM canola – How will it affect the way I farm? Murray Scholz, 2008 Nuffield scholar, Southern NSW

2. Eight years of IWM smashes tyegrass seed banks by 98% over 31 focus paddocks, Peter Newman, Glenn Adam & Trevor Bell, Department of Agriculture and Food

3. The global economic climate and impacts on agriculture, profile on Michael Whitehead Rabobank New York

4. Lessons from five years of cropping systems research, W.K. Anderson, Department of Agriculture and Food

5. Case study of a 17year old agricultural lime trial, C. Gazey, Department of Agriculture …

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Crop Updates 2009 - Cereals, Jeff Baldock, Doug Edmeades, Mark Seymour, Paul Carmody, Ian Pritchard, Alan Meldrum, Michael Robertson, Roger Lawes, Rob Sands, Peter White, Felicity Byrne, Andrew Bathgate, Kedar Adhikari, Tanveer Khan, Stuart Morgan, Alan Harris, P. Gaur, K. M. H. Siddique, H. Clarke, N. C. Turner, W. Macleod, S. Morgan, Chris Veitch, Tony Leonforte, Kithsiri Jayasena, Geoff J. Thomas, Rob Loughman, Kazue Tanaka, Ravjit Khangura, Mohammad Amjad, Richard Oliver, Dusty Severtson, Peter Mangano, John Botha, Brenda A. Coutts, Manisha Shankar, Kasia Rybak, Michael Baker, Andrea Hills, Shahajahan Miyan, Peter Portmann, Nicole Rice, Robert Henry, Jeff Russell, Blakely H. Paynter, Linda Price, Brenda Shackley, Vicki Scanlan, Darshan Sharma, Christine M. Zaicou-Kunesch

Crop Updates

This session covers twenty seven papers from different authors:

PLENARY

1. Building soil carbon for productivity and implications for carbon accounting, Jeff Baldock, CSIRO Land and Water, Adelaide, SA

2. Fact or Fiction: Who is telling the truth and how to tell the difference, Doug Edmeades, agKnowledge Ltd, Hamilton

3. Four decades of crop sequence trials in Western Australia, Mark Seymour,Department of Agriculture and Food

BREAK CROPS

4. 2008 Break Crops survey Report, Paul Carmody,Development Officer, Department of Agriculture and Food

5. Attitudes of Western Australian wheatbelt growers to ‘Break Crops’, Paul Carmody and Ian …

Crop Updates 2009 - Genetically Modified Crops, Nutrition, Soils, & Others, Wallace Cowling, Art Diggle, Caroline Peek, Frank D'Emden, Fiona Evans, Bob French, Rob Grima, Sam Harburg, Abul Hashem, John Holmes, Jeromy Lemon, Peter Newman, Janet Paterson, Steve Penny, Peter Portman, Mark Slatter, Angus Maclennan, Andrew Wells, Bill Bowden, Wayne Pluske, Wen Chen, Geoff Anderson, Ross Brennan, Richard Bell, Hannah Rigby, Deborah Pritchard, David Collins, Katrina Walton, David Allen, Nancy Penney, Michael Robertson, Roger Lawes, Stephen Davies, Chris Gazey, Breanne Best, David Gartner, Quenten Knight, Joel Andrew, Ryan Pearce, F. C. Hoyle, A. Bennett, Rick Llewellyn, Ken Flower, Julian Krieg, Owen Catto, Cindy Parsons

Crop Updates

This session covers fifteen papers from different authors:

1. Performance of Canola Breeders Roundup Ready^® canola hybrid CHYB-166 in 2008, Wallace Cowling, Canola Breeders Western Australia Pty Ltd

2. The implications of GM glyphosate resistant lupin, Art Diggle, Caroline Peek, Frank D’Emden, Fiona Evans, Bob French, Rob Grima, Sam Harburg, Abul Hashem,, John Holmes, Jeremy Lemon, Peter Newman, Janet Paterson, Steve Penny,Department of Agriculture and Food, Peter Portmann, Agriconnect

3. Nufarm Roundup Ready® Canola Systems Trials— 2008 Mark Slatter, Research and Development …

Genetic Control Of Sorghum Grain Color, René Clará Valencia, William L. Rooney

INTSORMIL Presentations

Today there is a real interest in the utilization of sorghum grain as a substitute for wheat in baked goods and as a substitute for yellow maize in animal feed. Sorghum grain color and quality are important characteristics in the baking and livestock feed industries. For that reason it is necessary to develop varieties that are suitable for the food and feed industries.

The purpose of this bulletin is to briefly describe the main genetic characteristics that objectively affect sorghum grain appearance and quality.

The pericarp and glume color affect the color of the finished products made with sorghum grain. …

Control Genético Del Color Del Grano De Sorgo, René Clará Valencia, William L. Rooney

INTSORMIL Presentations

Actualmente existe un gran interés en la utilización del grano de sorgo como fuente de energía en la elaboración de alimentos para el consumo humano y/o animal, sustituyendo al trigo en la panificación y al maíz amarillo en la elaboración de alimentos concentrados; para tal efecto es necesario conocer el control genético del color del grano para mejorar su calidad.

El objetivo de esta información es describir brevemente las características genéticas principales, actualmente conocidas y que afectan objetivamente la apariencia del grano de sorgo.

El color del pericarpio y la gluma de la flor afectan la coloración de los productos …

Current And Potential Distributions Of Three Non-Native Invasive Plants In The Contiguous Usa, Chad C. Jones, Sarah Reichard

Botany Faculty Publications

Biological invasions pose a serious threat to biodiversity, but monitoring for invasive species is time consuming and costly. Understanding where species have the potential to invade enables land managers to focus monitoring efforts. In this paper, we compared two simple types of models to predict the potential distributions of three non-native invasive plants (Geranium robertianum, Hedera spp., and Ilex aquifolium) in the contiguous USA. We developed models based on the climatic requirements of the species as reported in the literature (literature-based) and simple climate envelope models based on the climate where the species already occur (observation-based). We then compared the …

Roadmap For Future Research On Plant Pathogen Effectors, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

Bacterial and eukaryotic plant pathogens deliver effector proteins into plant cells to promote pathogenesis. Bacterial pathogens containing type III protein secretion systems are known to inject many of these effectors into plant cells. More recently, oomycete pathogens have been shown to possess a large family of effectors containing the RXLR motif, and many effectors are also being discovered in fungal pathogens. Although effector activities are largely unknown, at least a subset suppress plant immunity. A plethora of new plant pathogen genomes that will soon be available thanks to next-generation sequencing technologies will allow the identification of many more effectors. This …

Histone H3 Phosphorylation: Universal Code Or Lineage Specific Dialects?, Heriberto Cerutti, J. Armando Casas-Mollano

Center for Plant Science Innovation: Faculty Publications

Post-translational modifications of histones modulate the functional landscape of chromatin and impinge on many DNA-mediated processes. Phosphorylation of histone H3 plays a role in the regulation of gene expression and in chromosome condensation/segregation. Certain evolutionarily conserved residues on histone H3—namely Thr3, Ser10, Thr11, and Ser28—are phosphorylated during interphase or mitosis in both metazoa and plants. However, many of the kinases involved in these events appear to have evolved independently in different lineages. Likewise, the mechanistic function of specific phosphorylated amino acids, although poorly understood, also seems to differ among eukaryotes. Moreover, some modifications, such as phosphorylation of histone H3 Ser10, …

Protein-Protein Interactions Of Tandem Affinity Purified Protein Kinases From Rice, Jai S. Rohila, Mei Chen, Shuo Chen, Johann Chen, Ronald L. Cerny, Christopher Dardick, Patrick Canlas, Hiroaki Fujii, Michael Gribskov, Siddhartha Kanrar, Lucas Knoflicek, Becky Stevenson, Mingtang Xie, Xia Xu, Xianwu Zheng, Jing-Kang Zhu, Pamela Ronald, Michael E. Fromm

Center for Plant Science Innovation: Faculty Publications

Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAPtagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 …

Participation Of Leaky Ribosome Scanning In Protein Dual Targeting By Alternative Translation Initiation In Higher Plants, Yashitola Wamboldt, Saleem Mohammed, Christian Elowsky, Chris Wittgren, Wilson B. M. De Paula, Sally Ann Mackenzie

Center for Plant Science Innovation: Faculty Publications

Postendosymbiotic evolution has given rise to proteins that are multiply targeted within the cell. Various mechanisms have been identified to permit the expression of proteins encoding distinct N termini from a single gene. One mechanism involves alternative translation initiation (aTI). We previously showed evidence of aTI activity within the Arabidopsis thaliana organellar DNA polymerase gene POLg2. Translation initiates at four distinct sites within this gene, two non-AUG, to produce distinct plastid and mitochondrially targeted forms of the protein. To understand the regulation of aTI in higher plants, we used Polg2 as a model to investigate both cis- and trans …

Intergenic Transcription By Rna Polymerase Ii Coordinates Pol Iv And Pol V In Sirna-Directed Transcriptional Gene Silencing In Arabidopsis, Binglian Zheng, Zhengming Wang, Shengben Li, Bin Yu, Jin -Yuan Liu, Xuemei Chen

Center for Plant Science Innovation: Faculty Publications

Intergenic transcription by RNA Polymerase II (Pol II) is widespread in plant and animal genomes, but the functions of intergenic transcription or the resulting noncoding transcripts are poorly understood. Here, we show that Arabidopsis Pol II is indispensable for endogenous siRNA-mediated transcriptional gene silencing (TGS) at intergenic low-copy-number loci, despite the presence of two other polymerases—Pol IV and Pol V—that specialize in TGS through siRNAs. We show that Pol II produces noncoding scaffold transcripts that originate outside of heterochromatic, siRNA-generating loci. Through these transcripts and physical interactions with the siRNA effector protein ARGONAUTE4 (AGO4), Pol II recruits AGO4/siRNAs to homologous …

Letter From The Dean, Lalit Verma

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Determinants Of Chloroplast Gene Expression And Applications Of Chloroplast Transformation In Lactuca Sativa And Nicotiana Tabacum, Tracey Ruhlman

Electronic Theses and Dissertations

Genetic modification of plastids in the model plant tobacco (Nicotiana tabacum) has demonstrated that numerous foreign gene products can accumulate to high levels in this setting. Plastid biotechnology is maturing to encompass the improvement of food and feed species and the production of biopharmaceutical proteins for oral delivery necessitating development of stable transplastomic edible plants. In the interest of establishing an edible platform we have investigated the use of native and foreign regulatory elements in relation to foreign gene expression in plastids. Multiple sequence alignments of intergenic regions for 20 species of angiosperm showed that despite 95% identity in the …

Plan To Support Grains Industry Development 2009-2012, Department Of Agriculture And Food, Western Australia

Grain and other field crops published reports

The Plan to Support Grains Industry Development (‘the Plan’) recognises that Western Australian agricultural industries are integral to the economic wellbeing of regional and rural communities. The Plan promotes a progressive and profitable grains industry through modern best practice services and research to enhance continuous improvement in farming and supply chain practices.

The Plan has been developed to align with the Government of Western Australia’s initiatives, strategies and tactics for ‘making every hectare count’ (see Appendix 1) and with grains industry needs as identified by industry stakeholders during a series of consultation forums. These industry needs will be addressed in …