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Full-Text Articles in Plant Breeding and Genetics

Optimization Of Transformation And Regeneration In The Sunflower (Helianthus Annuus), Sara M. Kilar Jan 2025

Optimization Of Transformation And Regeneration In The Sunflower (Helianthus Annuus), Sara M. Kilar

Masters Theses

In addition to their value as prized ornamentals, sunflowers are important crop species due to the nutritional value and high oil content of the seeds. Sunflower oil is a significant contributor to global seed oil production, which can be used as a food additive and cooking agent as well as a raw material for biodiesel production. While traditional breeding efforts have led to the development of many unique cultivars, genetically modified variations have proven more difficult due to the recalcitrance of sunflower plants to genetic transformation. As a result, facile and efficient transformation methods for sunflowers have the potential to …


An Efficient Protocol For Root Studies In The Common Sunflower Using Composite Plants, Tyler Parks Jan 2018

An Efficient Protocol For Root Studies In The Common Sunflower Using Composite Plants, Tyler Parks

Masters Theses

Sunflower, Helianthus annuus, is one of the most important oilseed crop in the world. Oil from the seeds is prized for its' exceptional quality and flavor. Despite this, sunflowers have been forced onto marginal lands often on semi-arid and non-fertile soil, due to disease pressures and economics making it critical to produce more resilient lines and varieties to withstand these new stresses. The recent availability of the sunflower genome can allow genome-wide characterization of gene families. Stable transformation protocols, which can be used for characterization studies, have been developed for H. annuus using Agrobacterium tumefaciens, but they produce …


Investigation Of A Putative Membrane-Bound Protein In Arabidopsis Thaliana And Nicotiana Tabacum, Allen Potter Jan 2018

Investigation Of A Putative Membrane-Bound Protein In Arabidopsis Thaliana And Nicotiana Tabacum, Allen Potter

Masters Theses

The NLG (Novel Leaf Gene) is a recently discovered gene from aspen (Populus tremula x alba). Previous research indicates that NLG impacts leaf shape. Our preliminary analyses indicate that NLG may function as a membrane bound protein with possible localization in plasma membrane and/or nucleus. Sequence and phylogenetic analyses in Arabidopsis thaliana and Populus trichocarpa identified four homologous genes in Arabidopsis and five in Populus. Gene expression analysis was done using online tools and indicated that NLG is expressed in multiple tissue types but predominately in stem and flowers. The protein coding region of the NLG orthologs genes from aspen …


Reproductive Biology Of Stylisma Pickeringii (Convolvulaceae), An Endangered Plant Of Illinois Sand Prairies, Brent L. Todd Jan 2002

Reproductive Biology Of Stylisma Pickeringii (Convolvulaceae), An Endangered Plant Of Illinois Sand Prairies, Brent L. Todd

Masters Theses

The sand prairie is a unique ecosystem in the state of Illinois, being the habitat for many interesting plants and animals not found elsewhere in the state. One such plant is the state-endangered Stylisma pickeringii (Torr. ex M.A. Curtis) Gray var. pattersoni (Fern. & Schub.) Myint (Convolvulaceae), also known as Patterson's bindweed or Patterson's dawn flower. It currently is found in only three Illinois counties—Cass, Henderson, and Mason. Presently, two of the three populations of S. pickeringii are located on private lands. Very little information is known about the reproductive biology of S. pickeringii var. pattersoni. Thus, the purpose of …


Breeding Systems In Spiranthes Magnicamporum Sheviak, Christopher D. Loyet Jan 1993

Breeding Systems In Spiranthes Magnicamporum Sheviak, Christopher D. Loyet

Masters Theses

A study of the ecology, breeding systems, and pollinators of Spiranthes magnicamporum Sheviak was undertaken in fall 1992. An initial survey of three glacial drift hill prairies revealed sexual activity in this species via removed pollinia. Two hill prairie populations were used for pollination study. In each population, five individuals were randomly selected and crossed using autogamic, geitonogamic, and xenogamic methods. In addition, ten individuals were fitted with pollinator-exclusion bags. After capsule formation, all plants were harvested. They were examined in the lab for general morphology, the presence or absence of embryo formation, and the prescence of sexual or asexual …