Plant Breeding and Genetics Commons^™

Pseudomonas SyringaeHrpj Is A Type Iii Secreted Protein That Is Required For Plant Pathogenesis, Injection Of Effectors,And Secretion Of The Hrpz1 Harpin, Zheng Qing Fu, Ming Guo, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The bacterial plant pathogen Pseudomonas syringae requires a type III protein secretion system (TTSS) to cause disease. The P. syringae TTSS is encoded by the hrp-hrc gene cluster. One of the genes within this cluster, hrpJ, encodes a protein with weak similarity to YopN, a type III secreted protein from the animal pathogenic Yersinia species. Here, we show that HrpJ is secreted in culture and translocated into plant cells by the P. syringae pv. tomato DC3000 TTSS. A DC3000 hrpJ mutant, UNL140, was greatly reduced in its ability to cause disease symptoms and multiply in Arabidopsis thaliana …

Sheep Updates 2006 - Part 1, David Sackett, Kevin Foster, Ronald J. Yates, Phil Nichols, Graeme Martin, John Milton, Scott Williams, Garry Mcalister, Mark Suttie, Peter Fennessy, Jack Cocks

Sheep Updates

This session covers seven papers from different authors:

PLENARY

1. Making Dollars from Merinos, David Sackett, Holmes Sackett & Associates Pty Limited, Wagga Wagga, NSW

2. A new variety of sulla (Hedysarun coronarium)for forage production in southern Australia, Kevin Foster, Ron Yates, Phil Nichols, Department of Agriculture and Food, WA and Centre for Legumes in Mediterranean Agriculture, UWA

3. Mating - Short and fast is better, Graeme Martin, John Milton, Faculty of Natural & Agricultural Sciences, The University of Western Australia

4. Breech strike protection in sheep post 2010, Scott Williams, Program Manager Animal Health and Welfare, Australian Wool …

Effects Of Ultraviolet-B Radiation On Leaf Morphology Of Arabidopsis Thaliana (L.) Heynh. (Brassicaceae), Maria Regina Torres Boeger, Mary E. Poulson

Biology Faculty Scholarship

Reduction of the ozone layer results in the increase in ultraviolet radiation reaching the earth's surface, especially the ultraviolet-B (UV-B). The increase of radiation may induce structural and physiological changes in plants, influencing their growth and development. This paper evaluates the effects of ambient UV-B radiation upon to the leaf morphology of Arabidopsis thaliana developed under controlled conditions. The seeds of A. thaliana grown in environmental chamber, with 300 µmol m^-2s^-1 de photosynthetically active radiation (PAR) with and without 6 kJ m^-2 s^-1 of radiation UV-B_be (UV-B_be; UV-B biologically effective). After 21 …

Identifying Superior Hybrid Hazelnut Plants In Southeast Nebraska, Elizabeth A. Hammond

School of Natural Resources: Dissertations, Theses, and Student Research

The goal of this observational study is to identify productive hybrid hazelnut plants suitable for commercial production. The objectives of this studywere to 1) examine a plantation of hybrid hazelnut plants grown in Southeast Nebraska for the presence of eastern filbert blight (EFB); 2) identify high yielding hybrid hazelnut plants; and 3) characterize plant and nut features of selected high yielding plants.

Data were collected from a 3.64 hectare (9 acre) planting of 5,200 hybrid hazelnut plant located at Arbor Day Farm in Nebraska City, Otoe County, Nebraska. Plants were grown on 1.5 m x 4.6 m (5 foot x …

Crop Updates 2006 - Katanning, David Stephens, Michael Meuleners, Meredith Fairbanks, Thomas Schulz, T. J. Flowers, T. D. Colmer, Narelle Simpson, Ron Mctaggart, Wal Anderson, Lionel Martin, Dave Allen, Brenda Shackley, Raj Malik, Kelly Winfield, Rodger Beermier, Shahahan Miyan, Abul Hashem, Shahab Pathan, Vikki Osten, Alex Douglas, Garren Knell, Steve Curtin, Wade Longmuir, Henry Brockman, Justin Hardy, Arjen Ryder, John Paul Collins, Jessica Johns, John Pepall, Allan Herbert

Crop Updates

This session covers sixteen papers from different authors

1. 2006 SEASONAL OUTLOOK, David Stephens and Michael Meuleners, Department of Agriculture

2. Review of climate model summaries reported in the Department of Agriculture’s growing season outlooks, Meredith Fairbanks, Department of Agriculture

3. Farmers commodity outlook 2006, Thomas Schulz, Department of Agriculture

4. Why is salinity such a difficult problem for plant breeders? T J Flowers, TD Colmer, University of Western Australia

5. Matching nitrogen supply to wheat demand in 2005, Narelle Simpson, Ron McTaggart, Wal Anderson, Lionel Martin and Dave Allen, Department of Agriculture

6. Wheat varieties in 2006, Brenda Shackley, …

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Crop Updates 2006 - Cadoux And Calingiri, Raj Malik, Kelly Winfield, David Stephens, Michael Meuleners, Kari-Lee Falconer, Bill Bowden, Narelle Simpson, Anne Wilkins, Nathan Hancock, Peter Newman, Glenn Adam, Andrew Blake, Caroline Peak, Stephen Davies, Chris Gazey, Bob Gilkes, Dan Evans, Tania Liaghati

Crop Updates

This session covers nine papers from different authors

1. Performance of oaten hay varieties in Western Australian environments, Raj Malik and Kellie Winfield, Department of Agriculture

2. Performance of dwarf potential milling varieties in Western Australian environments, Raj Malik and Kellie Winfield, Department of Agriculture

3. 2006 Seasonal outlook, David Stephens, Michael Meuleners and Kari-Lee Falconer, Department of Agriculture

4. Matching nitrogen supply to crop demand in high rainfall cropping, Bill Bowden, Narelle Simpson Department of Agriculture

5. An overview of the potential for a Biofuels Industry in Western Australia, Anne Wilkins and Nathan Hancock, Department of Agriculture

6. IWM performs over 5 years …

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Crop Updates 2006 - Weeds, Alexandra Douglas, Thomas M. Wolf, Harm Van Rees, Bill Gordon, Peter Newman, Glenn Adam, Aik Lee, Siew Lee, Katherine Hollaway, Brad Rayner, John Peirce, Rick Llewellyn, Frank D'Emden, Michelle Owen, Stephen Powles, Michael Walsh, Emma Glasfurd, Kathryn Steadman, David Ferris, Bevan Addison, Aik Cheam, Dave Nicholson, Ruben Vargas, Shahab Pathan, Abul Hashem, Nerys Wilkins, Catherine Borger, Bob French, Kari-Lee Falconer, Martin Harries, Chris Matthews, Vikki Osten, Harnohinder Dhammu, David Nicholson, Richard Quinlan, Debbie Allen, Mark Seymour, Pam Burgess, Owen Coppen, Chris Roberts, Christiaan Valentine, A. K. Basandrai, W. J. Macleod, John Moore, Neil Rothnie, Russell Speed, John Andrew Simons, Ted Spadek, Daya Patabendige, Michael Renton, Sally Peltzer, Art Diggle

Crop Updates

This session covers thirty seven papers from different authors:

1. ACKNOWLEDGEMENTS, Alexandra Douglas, CONVENOR – WEEDS DEPARTMENT OF AGRICULTURE

SPRAY TECHNOLOGY

2. Meeting the variable application goals with new application technology, Thomas M. Wolf, Agriculture and Agri-Food Canada, Saskatoon Research Centre

3. Spray nozzles for grass weed control, Harm van Rees, BCG (Birchip Cropping Group)

4. Boom sprayer setups – achieving coarse droplets with different operating parameters, Bill Gordon, Bill Gordon Consulting

5. Complying with product label requirements, Bill Gordon, Bill Gordon Consulting

6. IWM a proven performer over 5 years in 33 focus paddocks, Peter Newman and …

Crop Updates 2006 - Farming Systems, Wayne Pluske, Bill Bowden, Craig Scanlan, Stephen Davies, Chris Gazey, Peter Tozer, Derk Bakker, Louise Barton, David Gatter, Renee Buck, Daniel Murphy, Christoph Hinz, Bill Porter, Meredith Fairbanks, Nicolyn Short, Ian Foster, James Fisher, Doug Abrecht, Mario D'Antuono, Tracey M. Gianatti, Paul Carmody, Frank D'Amden, Rick Llewellyn, Michael Burton, Caroline Peek, Nadine Eva, Chris Carter, Megan Abrahams, Andrew Blake, Paul Blackwell, Sylvian Pottier, Michael Robertson, Greg Lyle, Lisa Brennan, Tony J. Vyn, Simon Teakle, Peter Norris, Jeff Russell, James Fisher, Roy Murray-Prior, Deb Pritchard, Mike Collins, Greg Hamilton, Rob Hetherington, Andrew Van Burgel, Cliff Spann

Crop Updates

This session covers nineteen papers from different authors:

SOIL AND NUTRIENT MANAGEMENT

1. Inve$tigating fertili$er inve$tment, Wayne Pluske, Nutrient Management Systems

2. KASM, the potassium in Agricultural System Model,Bill Bowden and Craig Scanlan, DAWA Northam and UWA, School of Earth and Geographical Sciences

3. Long term productivity and economic benefits of subsurface acidity management from surface and subsurface liming, Stephen Davies, Chris Gazey and Peter Tozer, Department of Agriculture

4. Furrow and ridges to prevent waterlogging, Dr Derk Bakker, Department of Agriculture

5. Nitrous oxide emissions from a cropped soil in Western Australia, Louise Barton^{1 …}

Crop Updates 2006 - Lupins And Pulses, Peter White, Mark Seymour, Amelia Mclarty, Wayne Parker, Ian Pritchard, Bob French, Rodger Beermier, Bevan Buirchell, Mark Sweetingham, Jon Clements, Geoff Thomas, Roger Jones, Sofia Sipsas, John Quealy, Leigh Smith, Gordon Francis, Huaan Yang, Jeffrey Boersma, Margaret Pallotta, Chengdao Li, Krishnapillai Sivasithamparam, M. Nelson, H. Phan, S. Ellwood, P, Moolhuijzen, M. Bellgard, J. Hane, A. Williams, J. Fos-Nyarko, B. Wolko, M. Książkiewicz, M. Cakir, M. Jones, M. Scobie, C. O'Lone, S. J. Barker, R. Oliver, W. Cowling, Laurie Maiolo, Martin Harries, Jo Walker, Murray Blyth, Stephen Cosh, Dirranie Kirby, Kedar Adhikari, Patrizia Guantini, Katie Bell, Ken Adcock, Ciara Beard, Anne Smith, Tim Maling, Art Diggle, Debbie Thackray, Kadambot Siddique, Ping Si, Huaan Yang, Vijay Jayasena, Florence Kartawinata, Ranil Coorey, Ken Dods, Kerry Regan, Tanveer Khan, Jenny Garlinge, Rod Hunter, Pam Burges, Eric Armstrong, Glen Riethmuller, Greg Shea, Moin Salam, Jean Galloway, Pip Payne, Bill Macleod, Brenda Coutts, Pooran Gaur, Heather Clarke, Stuart Morgan, Alan Harris, Ted Knights, Kristy Hobson, Chris Matthews, Colin Hanbury, Tim Pope, Mike Baker, Leanne Young, Syed Muhammad Nasar-Abbas, Julie Plummer, D. Harris, Phill Chambers, Michael Materne, Dennis Van Gool, Huyen Phan, Simon Ellwood, J. Hane, Angela Williams, R. Ford, S. Thomas, R. Oliver, Rory Coffey, Chris Poole, Theo Pfaff, Judith Lichtenzveig, Lars Kamphuis, Nola D'Souza, Emma Groves, Karam Singh

Crop Updates

This session covers sixty six papers from different authors:

2005 LUPIN AND PULSE INDUSTRY HIGHLIGHTS

1. Lupin Peter White, Department of Agriculture

2. Pulses Mark Seymour, Department of Agriculture

3. Monthly rainfall at experimental sites in 2005

4. Acknowledgements Amelia McLarty EDITOR

5. Contributors

6. Background Peter White, Department of Agriculture

2005 REGIONAL ROUNDUP

7. Northern agricultural region Wayne Parker, Department of Agriculture

8. Central agricultural region Ian Pritchard and Bob French, Department of Agriculture

9. Great southern and lakes Rodger Beermier, Department of Agriculture

10. South east region Mark Seymour, Department of …

Crop Updates 2006 - Oilseeds, Graham Walton, Fiona Martin, Anne Wilkins, Nathan Hancock, Matthew Nelson, Marieclaire Castello, Linda Thompson, Anouska Cousin, Guijun Yan, Wallace Cowling, Moin Salam, Bill Mcloud, Ravjit Khangura, Jean Galloway, Art Diggle, R. F. Brennan, M. D. A. Bolland, P. M. Damon, Z. Rengel, Terry Rose, Qifu Ma, Dave Eksteen

Crop Updates

This session covers thirteen papers from different authors:

1. INTRODUCTION, Graham Walton, CONVENOR, Department of Agriculture

2. The performance of new TT canola varieties in National Variety Testing (NVT) WA, Fiona Martin, Research Agronomist, Agritech Crop Research

3. Comparison of TT Canola Varieties in Oilseeds WA Trials – 2005, Collated by G.H. Walton, Department of Agriculture, WA, from a collaboration between Oilseeds WA, Seed Companies, Agronomists and Growers

4. An overview of the potential for a Biofuels Industry in Western Australia, Anne Wilkins and Nathan Hancock, Department of Agriculture

5. Retrieval of fertile progeny from interspecific …

Crop Updates 2006 - Cereals, Roger Jones, Nichole Burges, Mohommad Amjad, Ben Curtis, Wal Anderson, Darren Chitty, Brianna Peake, Harm Van Rees, Cherie Reilly, James Hunt, Dean Holzworth, Zvi Hochman, Allan Peake, Neal Dalgliesh, Stephen Van Rees, Trudy Mccann, Peter Carberry, Raj Malik, Kelly Winfield, Brenda Shackley, Judith Devenish, Darshan Sharma, Steve Penny Jr, Christine Zaicou-Kunesch, Mike Bolland, Ross Brennan, Paul Damon, Zed Rengel, Jeremy Lemon, Perry Poulton, Nick Poole, Brooke Thompson, Stephen Loss, Jeff Russell, Angie Roe, James Eyres, Narelle Simpson, Ron Mctaggart, Lionel Martin, Dave Allen, Hossein Golzar, Manisha Shankar, Robert Loughman, John Majewski, Donna Foster, Jamie Piotrowski, Nicole Harry, Geoff Thomas, Kith Jayasena, Svetlana Micic, Paul Matson, Tony Dore, Thomas (Ben) Biddulph, Daryl Mares, Julie Plummer, Tim Setter, Garren Knell, Steve Curtin, Wade Longmuir, Nigel Metz, Tina Botwright Acuña, Len Wade, Cindy Mills, Peter Stone

Crop Updates

This session covers twenty nine papers from different authors:

PLENARY

1. The 2005 wheat streak mosaic virus epidemic in New South Wales and the threat posed to the Western Australian wheat industry, Roger Jones and Nichole Burges, Department of Agriculture

SOUTH COAST AGRONOMY

2. South coast wheat variety trial results and best options for 2006, Mohammad Amjad, Ben Curtis and Wal Anderson, Department of Agriculture

3. Dual purpose winter wheats to improve productivity, Mohammad Amjad and Ben Curtis, Department of Agriculture

4. South coast large-scale premium wheat variety trials, Mohammad Amjad and Ben Curtis, Department of Agriculture …

Evaluation Of Cauliflower And Broccoli Varieties: Summer Planting (November 2005 - February 2006), Department Of Food And Agriculture, Western Australia

Horticulture research reports

This document has been prepared from a cauliflower and broccoli variety evaluation trial conducted at the Manjimup Horticultural Research Institute, South Western Highway, Manjimup WA 6258. The results reflect the growth characteristics, yield, quality and density of the cauliflower and broccoli varieties as recorded at this site during the conditions of the growing period (November 2005 to February 2006).

This report is the first in a series of four produced from the evaluation of cauliflower and broccoli varieties conducted at the Manjimup Horticultural Research Institute.

The results are provided to allow seed suppliers, seedling nurseries, producers, packers and marketers to …

Facilitating The Development Of The Strawberry Industry In Western Australia, Dennis Phillips, Aileen Reid

Horticulture research reports

Horticulture Australia Project No. BS05001 Annual report 2005/06

This is the first of three annual reports for the Horticulture Australia project BS05001 — ‘Facilitating the development of the strawberry industry in Western Australia’. This project is managed by the Strawberry Growers Association of WA Inc. and the work is being done in collaboration with the Department of Agriculture and Food WA (DAFWA). The project has been funded by voluntary contributions from the W.A. Strawberry Producers Committee, Toolangi Strawberry Runner Growers Co-operative and DAFWA with matching funding from Horticulture Australia Ltd (HAL). The project has been funded for three years, 2005, …

Australian Temperate Strawberry Breeding Program (Western Australia) Selection Of New And Advanced Short Day Breeding Lines And Cultivars In Western Australia - 2005/2006, Michaela Antoine, Dennis Phillips

Horticulture research reports

Sixty-eight 'short day' selections from the Australian Temperate breeding program were compared in a trial planting on a commercial property at Wanneroo from a mid May planting. Thirty-three of the selections were derived from crosses made in 2003 (new Stage 4 selections) and twenty-nine were from crosses made between 1998 and 2002 (Intermediate Stages 5 and 6). In addition, three advanced selections were compared in large plots of 1,000-1 ,500 plants together with three commercial control cultivars, Gaviota, Camino Real and Kiewa. These selections were 'fast tracked' for future commercialisation by being selected for bulking up in September 2004.

Seven …

A Highly Efficient Transient Protoplast System For Analyzing Defence Gene Expression And Protein–Protein Interactions In Rice, Songbiao Chen, Lizen Tao, Lirong Zeng, Miguel E. Vega-Zanchez, Kenji Umemura, Guo-Liang Wang

Center for Plant Science Innovation: Faculty Publications

The transient assay system based on mesophyll or cultured cell-derived protoplasts has been exploited in several plant species and has become a powerful tool for rapid gene functional analysis and biochemical manipulations. However, the system has not been widely used in rice owing to the difficulties in large-scale isolation of viable rice protoplasts from leaves or suspension-cultured cells. Here, we describe a significantly improved method to isolate a large number of protoplasts from stem and sheath tissues of both young and mature plants. High-level coexpression of multiple constructs and efficient suppression of exogenous and endogenous genes were observed in the …

Evaluation Of Cauliflower And Broccoli Varieties: Autumn Planting (January - May 2006), Kristen Stirling

Horticulture research reports

This document has been prepared from a cauliflower and broccoli variety evaluation trial conducted at the Manjimup Horticultural Research Institute, South Western Highway, Manjimup WA 6258. The results reflect the growth characteristics, yield, quality and density of the cauliflower and broccoli varieties as recorded at this site during the conditions of the growing period (January to May 2006).

This report is the second in a series of four produced from the evaluation of cauliflower and broccoli varieties conducted at the Manjimup Horticultural Research Institute. The results are provided to allow seed suppliers, seedling nurseries, producers, packers and marketers to identify …

Evaluation Of Cauliflower And Broccoli Varieties: Winter Planting (April - August 2006), Kristen Stirling

Horticulture research reports

This document has been prepared from a cauliflower and broccoli variety evaluation trial! conducted at the Manjimup Horticultural Research Institute, South Western Highway, Manjimup WA 6258. The results reflect the growth characteristics, yield, quality and density of the cauliflower and broccoli varieties as recorded at this site during the conditions of the growing period (April - August 2006).

This report is the third in a series of four produced from the evaluation of cauliflower and broccoli varieties conducted at the Manjimup Horticultural Research Institute. The results are provided to allow seed suppliers, seedling nurseries, producers, packers and marketers to identify …

Genetic Diversity And Gene Flow In Fragmented Populations Of The Rare Shrub, Calothamnus Sp. Whicher, Sean Stankowski

Theses : Honours

Ca/othamnus sp. Whicher (Myrtaceae) is a narrow range endemic shrub restricted to ironstone soils near the town of Busselton in south-western Australia. Due to extensive land clearing for agriculture and mineral exploration, the species is fragmented over the majority of its range. In the present study, microsatellite markers were used to characterise levels of genetic diversity and describe levels of differentiation and gene flow among seven small, isolated road verge populations. Allelic diversity within the taxon over the six microsatellites was high (A = 17.6 ± 1.6). Diversity within populations was considerably lower (A = 5.19 ± 1.27), …

Development Of In Vitro Regeneration Protocols For Arkansas Rice Varieties (Oryza Sativa L.), Moytri Roy Chowdhury, Audrei Dabul, John Hubstenberger, Gregory Phillips

Journal of the Arkansas Academy of Science

Efficient regeneration of in vitro cultures of Oryza sativa L.is essential for successful manipulation of recombinant DNA technologies. Arkansas rice varieties perform better on modified Gamborg (B5)media, than on Murashige and Skoog (MS) orN6 media, which are more frequently reported in the literature. While 2,4-dichlorophenoxyacetic acid (2,4-D) is commonly used for regeneration treatments of rice, Picloram (Pic) provides a possible alternative as a synthetic auxin source. Although regeneration frequency appears low, complete regeneration (whole plantlets) was faster and development of the shoot was superior on picloram treatments as compared to 2,4-D. Screening 5 Arkansas varieties for regeneration efficiency on 3 …

Pollen Characteristics Of Grevillea Species Determined By In Vitro Germination, Rebecca Parsons

Theses : Honours

Germination of pollen in vitro is a common technique used to assess the ability of pollen to germinate under a variety of conditions. The ability to determine the viability of a pollen grain, and optimal conditions for maximum germination and storage are important for hybridisation. A means of storing pollen, while maintaining viability, enables inter-species hybridisation between species that are spatially and temporally (flowering time) separated. The ultimate aim of hybridisation is seed set and in order to increase this in a genus renowned for a very low fruit to flower ratio, maximum pollen germination must be obtained. Therefore, by …

Adventitious Shoot Propagation And Cultural Inputs In Nursery Production Of A Primocane-Fruiting Blackberry Selection, Kimberley Dennis, John R. Clark, James A. Robbins

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Studies were conducted from January to October 2005 to determine the effect of root-cutting length on adventitious shoot yield and the management practices necessary to produce nursery-quality primocane-fruiting blackberry plants. The first portion of the study measured the average number of shoots produced from 7.6 cm- and 15.2 cm-long root cuttings of APF-44 blackberry—a primocane-fruiting genotype from the University of Arkansas breeding program. Cuttings were forced in a shallow bin containing a soilless potting medium. The average number of shoots per root cutting from 7.6 cm- and 15.2 cm- long root cuttings averaged 1.6 and 2.7 shoots per root cutting, …

Evaluation Of Cross Pollination Of Zephyranthes And Habranthus Species And Hybrids, Moytri Roy Chowdhury, John Hubstenberger

Journal of the Arkansas Academy of Science

The genus Zephyranthes and the related genus Habranthus in the family Amaryllidaceae are commonly grown ornamental bulbs having new world homologies. Inter-specific and inter-generic hybridizations are complicated by the fact that some of the species are apomictic, cross incompatible, or have widely variable 2n chromosome numbers. A simple, inexpensive method of pollen storage was tested to evaluate the production of hybrid seed. Intact anthers harvested after pollen release and stored at 4°C were used for pollination. Emasculated flowers were pollinated at noon on the first or second day of anthesis. Hybrid crosses were labeled and seed collected when formed. All …

A Guide For Skeleton Weed Management And Control, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

Assisting Western Australian landholders to eradicate skeleton weed and to prevent its further spread within the State