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Department of Primary Industries and Regional Development, Western Australia

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Full-Text Articles in Plant Breeding and Genetics

Adaptation Of The Australian Parastagonospora Nodorum Population To Alterations In The Host, Huyen T T Phan, Manisha Shankar, Darcy A B Jones, Eiko Furuki, Kasia Rybak, Fiona Kamphuis, Hossein Golzar, Richard P. Oliver Nov 2026

Adaptation Of The Australian Parastagonospora Nodorum Population To Alterations In The Host, Huyen T T Phan, Manisha Shankar, Darcy A B Jones, Eiko Furuki, Kasia Rybak, Fiona Kamphuis, Hossein Golzar, Richard P. Oliver

Grain and Other Field Crops Research Articles

Septoria nodorum blotch (SNB) is an economically important fungal disease of wheat caused by Parastagonospora nodorum. It is primarily controlled by the breeding of resistant wheat cultivars, but experience over the last 50 years shows that new pathogen populations soon evolve that are more virulent on the current popular cultivars. In this study, we assembled a panel of 360 P. nodorum isolates. The collection resolved into eight subpopulations. One core and seven transient populations were found possessing contrasting characters in term of spatial and temporal distribution, mating-type, effector haplotypes and patterns of intact and degraded copies of a Tc-1 mariner …


Lethal And Sublethal Effects Of Cotton Expressing Single And Pyramided Proteins Of Bacillus Thuringiensis (Bt) On Helicoverpa Armigera (Lepidoptera: Noctuidae), Spodoptera Litura (Lepidoptera: Noctuidae), And Spodoptera Frugiperda (Lepidoptera: Noctuidae), Sharna Holman, Paul Grundy, Helen Spafford, Michael Furlong May 2025

Lethal And Sublethal Effects Of Cotton Expressing Single And Pyramided Proteins Of Bacillus Thuringiensis (Bt) On Helicoverpa Armigera (Lepidoptera: Noctuidae), Spodoptera Litura (Lepidoptera: Noctuidae), And Spodoptera Frugiperda (Lepidoptera: Noctuidae), Sharna Holman, Paul Grundy, Helen Spafford, Michael Furlong

Grain and Other Field Crops Research Articles

The susceptibility to proteins from Bacillus thuringiensis (Bt) can vary among lepidopteran pest species. While Bollgard 3 cotton (BG3) effectively controls the primary pest Helicoverpa armigera (Hübner) in Australia, its effectiveness against other pests, such as Spodoptera litura (Fabricius) and Spodoptera frugiperda (J. E. Smith), is unknown. This laboratory study assessed the survival and development of H. armigera, S. litura, and S. frugiperda larvae when fed foliage from a non-transgenic cotton variety (CC) and 3 transgenic cotton varieties: Bollgard (BG1) expressing Cry1Ac, Bollgard II (BG2) expressing Cry1Ac and Cry2Ab, and Bollgard 3 (BG3) expressing Cry1Ac, Cry2Ab, and Vip3A. Pyramided …


Hordeum I Genome Unlocks Adaptive Evolution And Genetic Potential For Crop Improvement, Hao Feng, Qingwei Du, Ying Jiang, Yong Jia, Tianhua He, Yibin Wang, Brett Chapman, Jiaxin Yu, Hiawen Zhang, Mengxue Gu, Mengwei Jiang, Shanshan Gao, Xinjie Zhang, Yameng Song, Vanika Garg, Rajeev K. Varshney, Jianhua Wei, Chengdao Li, Xingtan Zhang, Ruifen Li Mar 2025

Hordeum I Genome Unlocks Adaptive Evolution And Genetic Potential For Crop Improvement, Hao Feng, Qingwei Du, Ying Jiang, Yong Jia, Tianhua He, Yibin Wang, Brett Chapman, Jiaxin Yu, Hiawen Zhang, Mengxue Gu, Mengwei Jiang, Shanshan Gao, Xinjie Zhang, Yameng Song, Vanika Garg, Rajeev K. Varshney, Jianhua Wei, Chengdao Li, Xingtan Zhang, Ruifen Li

Grain and Other Field Crops Research Articles

Crop wild relatives (CWRs) are invaluable for crop improvement. Among these, Hordeum I-genome species exhibit exceptional tolerance to alkali and salt stresses. Here we present a chromosome-scale genome assembly of Hordeum brevisubulatum (II, 2n = 2x =14) and genome resequencing of 38 diploid germplasms spanning 7 I-genome species. We reveal that the adaptive evolution of the H. brevisubulatum genome is shaped by structural variations, some of which may contribute to its adaptation to high alkali and salt environments. Evolutionary duplication of the stress sensor-responder module CaBP-NRT2 and the horizontally transferred fungal gene Fhb7 were identified as novel alkaline–saline …


A Barley Pan-Transcriptome Reveals Layers Of Genotype-Dependent Transcriptional Complexity, Wenbin Guo, Miriam Schreiber, Vanda B. Marosi, Paolo Bagnaresi, Morten Egevang Jorgensen, Katarzyna B. Braune, Ken J. Chalmers, Brett Chapman, Viet Hoang Dang, Christoph Dockter, Anne Fiebig, Geoffrey B. Fincher, Agostino Fricano, John Fuller, Allison Haaning, Georg Haberer, Axel Himmelbach, Murukarthick Jayakodi, Yong Jia, Nadia Kamal, Peter Langridge, Chengdao Li Feb 2025

A Barley Pan-Transcriptome Reveals Layers Of Genotype-Dependent Transcriptional Complexity, Wenbin Guo, Miriam Schreiber, Vanda B. Marosi, Paolo Bagnaresi, Morten Egevang Jorgensen, Katarzyna B. Braune, Ken J. Chalmers, Brett Chapman, Viet Hoang Dang, Christoph Dockter, Anne Fiebig, Geoffrey B. Fincher, Agostino Fricano, John Fuller, Allison Haaning, Georg Haberer, Axel Himmelbach, Murukarthick Jayakodi, Yong Jia, Nadia Kamal, Peter Langridge, Chengdao Li

Grain and Other Field Crops Research Articles

A pan-transcriptome describes the transcriptional and post-transcriptional consequences of genome diversity from multiple individuals within a species. We developed a barley pan-transcriptome using 20 inbred genotypes representing domesticated barley diversity by generating and analyzing short- and long-read RNA-sequencing datasets from multiple tissues. To overcome single reference bias in transcript quantification, we constructed genotype-specific reference transcript datasets (RTDs) and integrated these into a linear pan-genome framework to create a pan-RTD, allowing transcript categorization as core, shell or cloud. Focusing on the core (expressed in all genotypes), we observed significant transcript abundance variation among tissues and between genotypes driven partly by RNA …


Diversity Of Gibberellin 2-Oxidase Genes In The Barley Genome Offers Opportunities For Genetic Improvement, Jingye Cheng, Yong Jia, Camilla Hill, Tianhua He, Ke Wang, Ganggang Guo, Sergey Shabala, Meixue Zhou, Yong Han, Chengdao Li Dec 2024

Diversity Of Gibberellin 2-Oxidase Genes In The Barley Genome Offers Opportunities For Genetic Improvement, Jingye Cheng, Yong Jia, Camilla Hill, Tianhua He, Ke Wang, Ganggang Guo, Sergey Shabala, Meixue Zhou, Yong Han, Chengdao Li

Grain and Other Field Crops Research Articles

Introduction

Gibberellin (GA) is a vital phytohormone in regulating plant growth and development. During the “Green Revolution”, modification of GA-related genes created semi-dwarfing phenotype in cereal crops but adversely affected grain weight. Gibberellin 2-oxidases (GA2oxs) in barley act as key catabolic enzymes in deactivating GA, but their functions are still less known.

Objectives

This study investigates the physiological function of two HvGA2oxgenes in barley and identifies novel semi-dwarf alleles with minimum impacts on other agronomic traits.

Methods

Virus-induced gene silencing and CRISPR/Cas9 technology were used to manipulate gene expression of HvGA2ox9 and HvGA2ox8ain barley and RNA-seq …


Pangenome And Pantranscriptome As The New Reference For Gene-Family Characterization: A Case Study Of Basic Helix-Loop-Helix (Bhlh) Genes In Barley, Cen Tong, Yong Jia, Haifei Hu, Zhanghui Zeng, Brett Chapman, Chengdao Li Nov 2024

Pangenome And Pantranscriptome As The New Reference For Gene-Family Characterization: A Case Study Of Basic Helix-Loop-Helix (Bhlh) Genes In Barley, Cen Tong, Yong Jia, Haifei Hu, Zhanghui Zeng, Brett Chapman, Chengdao Li

Grain and Other Field Crops Research Articles

Genome-wide identification and comparative gene-family analyses have commonly been performed to investigate species-specific evolution linked to various traits and molecular pathways. However, most previous studies have been limited to gene screening in a single reference genome, failing to account for the gene presence/absence variations (gPAVs) in a species. Here, we propose an innovative pangenome-based approach for gene-family analyses based on orthologous gene groups (OGGs). Using the basic helix-loop-helix (bHLH) transcription factor family in barley as an example, we identified 161–176 bHLHs in 20 barley genomes, which can be classified into 201 OGGs. These 201 OGGs were further classified into 140 …


Are You Planting Resistant Weeds With Your Crop Seed?, Department Of Primary Industries And Regional Development, Western Australia Sep 2024

Are You Planting Resistant Weeds With Your Crop Seed?, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Key messages

• West Australian (WA) grain growers have significant weed seed contamination in their crop seed.

• WA growers are sowing herbicide resistant weed seed into their cropping paddocks.

• Crop hygiene (harvest, seeding, transporting) is important to minimise spread of weeds.

Harvest is well underway throughout the WA grainbelt, and growers will be harvesting grain and collecting seed for next year’s crop.

However, this crop seed can be contaminated with weed seeds and, if seed cleaning operations are only partially effective, this weed seed may be sown into paddocks with crop seed in the following season.


Transcriptome-Wide Genetic Variations In The Legume Genus Leucaena For Fingerprinting And Breeding, Yong Han, Alexander Abair, Julian Van Der Zanden, Madhugiri Nageswara-Rao, Saipriyaa Purushotham Vasan, Roopali Bhoite, Marieclaire Castello, Donovan Bailey, Clinton K. Revell Dr, Chengdao Li, Daniel Real Jul 2024

Transcriptome-Wide Genetic Variations In The Legume Genus Leucaena For Fingerprinting And Breeding, Yong Han, Alexander Abair, Julian Van Der Zanden, Madhugiri Nageswara-Rao, Saipriyaa Purushotham Vasan, Roopali Bhoite, Marieclaire Castello, Donovan Bailey, Clinton K. Revell Dr, Chengdao Li, Daniel Real

Grain and Other Field Crops Research Articles

Leucaena is a versatile legume shrub/tree used as tropical livestock forage and in timber industries, but it is considered a high environmental weed risk due to its prolific seed production and broad environmental adaptation. Interspecific crossings between Leucaena species have been used to create non-flowering or sterile triploids that can display reduced weediness and other desirable traits for broad use in forest and agricultural settings. However, assessing the success of the hybridisation process before evaluating the sterility of putative hybrids in the target environment is advisable. Here, RNA sequencing was used to develop breeding markers for hybrid parental identification in …


The De-Domestication Of Ornithopus Sativus Brot. To Develop Cultivars With Physical Dormancy (Hardseed), Robert J. Harrison, Bradley J. Nutt, Ronald J. Yates, Belinda F. Hackney, John G. Howieson May 2024

The De-Domestication Of Ornithopus Sativus Brot. To Develop Cultivars With Physical Dormancy (Hardseed), Robert J. Harrison, Bradley J. Nutt, Ronald J. Yates, Belinda F. Hackney, John G. Howieson

Grain and Other Field Crops Research Articles

Ornithopus sativus Brot. (French serradella) is a forage legume that is well adapted to acidic coarse textured soils (sands) which are characterized by poor nutrition and an inability to retain water. During the process of domestication of O. sativus, there was an unintentional loss of seed physical dormancy (PY) thus compromising its self-regeneration after a cropping interval. Through mass screening of seed, we identified for the first time that heritable sources of PY exist in three populations of O. sativus. This rare genetic material was then incorporated into suitable genetic backgrounds of differing maturity through targeted hybridization. We demonstrated …


Advances And Opportunities In Unraveling Cold-Tolerance Mechanisms In The World's Primary Staple Food Crops, Sofora Jan, Sachin Rustgi, Rutwik Barmukh, Asif B. Shikari, Brenton Leske, Amanuel Bekuma, Darshan L. Sharma, Wujun Ma, Upendra Kumar, Uttam Kumar, Abhishek Bohra, Rajeev K. Varshney, Reyazul Rouf Mir Nov 2023

Advances And Opportunities In Unraveling Cold-Tolerance Mechanisms In The World's Primary Staple Food Crops, Sofora Jan, Sachin Rustgi, Rutwik Barmukh, Asif B. Shikari, Brenton Leske, Amanuel Bekuma, Darshan L. Sharma, Wujun Ma, Upendra Kumar, Uttam Kumar, Abhishek Bohra, Rajeev K. Varshney, Reyazul Rouf Mir

Grain and Other Field Crops Research Articles

Temperatures below or above optimal growth conditions are among the major stressors affecting productivity, end-use quality, and distribution of key staple crops including rice (Oryza sativa), wheat (Triticum aestivum), and maize (Zea mays L.). Among temperature stresses, cold stress induces cellular changes that cause oxidative stress and slowdown metabolism, limit growth, and ultimately reduce crop productivity. Perception of cold stress by plant cells leads to the activation of cold-responsive transcription factors and downstream genes, which ultimately impart cold tolerance. The response triggered in crops to cold stress includes gene expression/suppression, the accumulation of sugars upon chilling, and signaling molecules, among …


Exome-Based New Allele-Specific Pcr Markers And Transferability For Sodicity Tolerance In Bread Wheat (Triticum Aestivum L.), Roopali Bhoite, Rosemary Smith, Urmil Bansal, Mirza Dowla, Harbans Bariana, Darshan L. Sharma Aug 2023

Exome-Based New Allele-Specific Pcr Markers And Transferability For Sodicity Tolerance In Bread Wheat (Triticum Aestivum L.), Roopali Bhoite, Rosemary Smith, Urmil Bansal, Mirza Dowla, Harbans Bariana, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Targeted exome-based genotype by sequencing (t-GBS), a sequencing technology that tags SNPs and haplotypes in gene-rich regions was used in previous genome-wide association studies (GWAS) for sodicity tolerance in bread wheat. Thirty-nine novel SNPs including 18 haplotypes for yield and yield-components were identified. The present study aimed at developing SNP-derived markers by precisely locating new SNPs on ~180 bp allelic sequence of t-GBS, marker validation, and SNP functional characterization based on its exonic location. We identified unknown locations of significant SNPs/haplotypes by aligning allelic sequences on to IWGSC RefSeqv1.0 on respective chromosomes. Eighteen out of the target 39 SNP locations …


Genetic Resources And Precise Gene Editing For Targeted Improvement Of Barley Abiotic Stress Tolerance, Sakura Karunarathne, Esther Walker, Darshan Sharma, Chengdao Li, Yong Han Jul 2023

Genetic Resources And Precise Gene Editing For Targeted Improvement Of Barley Abiotic Stress Tolerance, Sakura Karunarathne, Esther Walker, Darshan Sharma, Chengdao Li, Yong Han

Grain and Other Field Crops Research Articles

Abiotic stresses, predominately drought, heat, salinity, cold, and waterlogging, adversely affect cereal crops. They limit barley production worldwide and cause huge economic losses. In barley, functional genes under various stresses have been identified over the years and genetic improvement to stress tolerance has taken a new turn with the introduction of modern gene-editing platforms. In particular, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a robust and versatile tool for precise mutation creation and trait improvement. In this review, we highlight the stress-affected regions and the corresponding economic losses among the main barley producers. We collate about …


Genomic Approaches To Enhance Adaptive Plasticity To Cope With Soil Constraints Amidst Climate Change In Wheat, Roopali Bhoite, Yong Han, Alamuru Krishna Chaitanya, Rajeev K. Varshney, Darshan L. Sharma Jun 2023

Genomic Approaches To Enhance Adaptive Plasticity To Cope With Soil Constraints Amidst Climate Change In Wheat, Roopali Bhoite, Yong Han, Alamuru Krishna Chaitanya, Rajeev K. Varshney, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Climate change is varying the availability of resources, soil physicochemical properties, and rainfall events, which collectively determines soil physical and chemical properties. Soil constraints—acidity (pH < 6), salinity (pH ≤ 8.5), sodicity, and dispersion (pH > 8.5)—are major causes of wheat yield loss in arid and semiarid cropping systems. To cope with changing environments, plants employ adaptive strategies such as phenotypic plasticity, a key multifaceted trait, to promote shifts in phenotypes. Adaptive strategies for constrained soils are complex, determined by key functional traits and genotype × environment × management interactions. The understanding of the molecular basis of stress tolerance is particularly challenging for plasticity traits. Advances in sequencing and high-throughput genomics …


An International Wheat Diversity Panel Reveals Novel Sources Of Genetic Resistance To Tan Spot In Australia, Julian Taylor, Dorthe Jorgensen, Caroline S. Moffat, Ken J. Chalmers, Rebecca Fox, Grant J. Hollaway, Melissa J. Cook, Stephen M. Neate, Pao Theen See, Manisha Shankar Mar 2023

An International Wheat Diversity Panel Reveals Novel Sources Of Genetic Resistance To Tan Spot In Australia, Julian Taylor, Dorthe Jorgensen, Caroline S. Moffat, Ken J. Chalmers, Rebecca Fox, Grant J. Hollaway, Melissa J. Cook, Stephen M. Neate, Pao Theen See, Manisha Shankar

Biosecurity Research Articles

Tan spot is a foliar disease in wheat caused by the fungal pathogen Pyrenophora tritici-repentis (Ptr) and has been reported to generate up to 50% yield losses under favourable disease conditions. Although farming management practices are available to reduce disease, the most economically sustainable approach is establishing genetic resistance through plant breeding. To further understand the genetic basis for disease resistance, we conducted a phenotypic and genetic analysis study using an international diversity panel of 192 wheat lines from the Maize and Wheat Improvement Centre (CIMMYT), the International Centre for Agriculture in the Dry Areas (ICARDA) and Australian (AUS) wheat …


New Semi-Dwarfing Alleles With Increased Coleoptile Length By Gene Editing Of Gibberellin 3-Oxidase 1 Using Crispr-Cas9 In Barley (Hordeum Vulgare L.), Jingye Cheng, Camilla Hill, Yong Han, Tianhua He, Xingguo Ye, Sergey Shabala, Ganggang Guo, Meixue Zhou, Ke Wang, Chengdao Li Dec 2022

New Semi-Dwarfing Alleles With Increased Coleoptile Length By Gene Editing Of Gibberellin 3-Oxidase 1 Using Crispr-Cas9 In Barley (Hordeum Vulgare L.), Jingye Cheng, Camilla Hill, Yong Han, Tianhua He, Xingguo Ye, Sergey Shabala, Ganggang Guo, Meixue Zhou, Ke Wang, Chengdao Li

Grain and Other Field Crops Research Articles

The green revolution was based on genetic modification of the gibberellin (GA) hormone system with “dwarfing” gene mutations that reduces GA signals, conferring shorter stature, thus enabling plant adaptation to modern farming conditions. Strong GA-related mutants with shorter stature often have reduced coleoptile length, discounting yield gain due to their unsatisfactory seedling emergence under drought conditions. Here we present gibberellin (GA) 3-oxidase1 (GA3ox1) as an alternative semi-dwarfing gene in barley that combines an optimal reduction in plant height without restricting coleoptile and seedling growth. Using large-scale field trials with an extensive collection of barley accessions, we showed that a natural …


Successful Creation Of Seedless (Sterile) Leucaena Germplasm Developed From Interspecific Hybridisation For Use As Forage, Daniel Real, Clinton K. Revell Dr, Yong Han, Chengdao Li, Marieclaire Castello, C. D. Bailey Dec 2022

Successful Creation Of Seedless (Sterile) Leucaena Germplasm Developed From Interspecific Hybridisation For Use As Forage, Daniel Real, Clinton K. Revell Dr, Yong Han, Chengdao Li, Marieclaire Castello, C. D. Bailey

Grain and Other Field Crops Research Articles

Context: The legume shrub/tree leucaena (Leucaena leucocephala (Lam.) de Wit.) is highly regarded as a cattle fodder, although it is considered an environmental weed in many parts of Australia.

Aims: We investigated the feasibility of developing a forage variety of triploid leucaena through interspecific hybridisation that is sterile (without seeds) as a strategy to mitigate its weed risk.

Methods: A conventional breeding strategy was used to create triploid hybrids from hand-crosses between tetraploid and diploid species of leucaena. Molecular markers were developed to confirm successful crosses and flow cytometry was used to confirm the ploidy level. The plants are being …


2023 Western Australian Crop Sowing Guide, B. Shackley, B. Paynter, J. Bucat, M. Seymour, H. Dhammu Oct 2022

2023 Western Australian Crop Sowing Guide, B. Shackley, B. Paynter, J. Bucat, M. Seymour, H. Dhammu

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Welcome to the 2023 edition of the Crop Sowing Guide for WA, which introduces 31 new variety releases: two wheat, seven barley (one as feed only and six currently under malt evaluation), 15 canola, three hay-only oat and four lentil varieties.

The Crop Sowing Guide for WA has been compiled by officers in the Department of Primary Industries and Regional Development. It provides information to support variety decisions for each of the major crops for the upcoming season.

In this edition, the canola NVT series are now identified by GRDC/NVT as ‘Low-Med Rainfall’ and ‘Med–High Rainfall’ to reflect the environments …


Molecular Pathways Of Wrky Genes In Regulating Plant Salinity Tolerance, Lewis Price, Yong Han, Tefera Angessa, Chengdao Li Sep 2022

Molecular Pathways Of Wrky Genes In Regulating Plant Salinity Tolerance, Lewis Price, Yong Han, Tefera Angessa, Chengdao Li

Grain and Other Field Crops Research Articles

Salinity is a natural and anthropogenic process that plants overcome using various responses. Salinity imposes a two-phase effect, simplified into the initial osmotic challenges and subsequent salinity-specific ion toxicities from continual exposure to sodium and chloride ions. Plant responses to salinity encompass a complex gene network involving osmotic balance, ion transport, antioxidant response, and hormone signalling pathways typically mediated by transcription factors. One particular transcription factor mega family, WRKY, is a principal regulator of salinity responses. Here, we categorize a collection of known salinity-responding WRKYs and summarize their molecular pathways. WRKYs collectively play a part in regulating osmotic balance, ion …


Adaptation Of Quinoa (Chenopodium Quinoa Willd.) To Australian Environments, Richard Snowball, Harmohinder S. Dhammu, Mario Francesco D'Antuono, David Troldahl, Ian Biggs, Callen Thompson, Mark Warmington, Amanda Pearce, Darshan L. Sharma Aug 2022

Adaptation Of Quinoa (Chenopodium Quinoa Willd.) To Australian Environments, Richard Snowball, Harmohinder S. Dhammu, Mario Francesco D'Antuono, David Troldahl, Ian Biggs, Callen Thompson, Mark Warmington, Amanda Pearce, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Quinoa is being evaluated in cropping systems in many countries outside of its natural range of South America. Very few attempts have been made by farmers or researchers to grow or evaluate quinoa under Australian environments. Given the growing popularity of quinoa with consumers, new commercial opportunities for farmers and international interest in the crop, it was timely to undertake a comprehensive evaluation of the potential of quinoa in Australia. Two advanced selections and nine germplasm lines (six of Chilean and three of Bolivian origin) identified in an earlier project were tested in 23 field trials at 14 locations on …


Genome Architecture And Diverged Selection Shaping Pattern Of Genomic Differentiation In Wild Barley, Wenying Zhang, Cong Tan, Haifei Hu, Rui Pan, Yuhui Xiao, Kai Ouyang, Yong Jia, Xiao-Qi Zhang, Camilla Hill, Penghao Wang, Brett Chapman, Yong Han, Gaofeng Zhou, Le Xu, Yanhao Xu, Tefera Angessa, Hao Luo, Sharon Westcott, Darshan Sharma, Eviatar Nevo, Roberto Barrero, Matthew I. Bellgard, Tianhua He, Xiaohai Tian, Chengdao Li Aug 2022

Genome Architecture And Diverged Selection Shaping Pattern Of Genomic Differentiation In Wild Barley, Wenying Zhang, Cong Tan, Haifei Hu, Rui Pan, Yuhui Xiao, Kai Ouyang, Yong Jia, Xiao-Qi Zhang, Camilla Hill, Penghao Wang, Brett Chapman, Yong Han, Gaofeng Zhou, Le Xu, Yanhao Xu, Tefera Angessa, Hao Luo, Sharon Westcott, Darshan Sharma, Eviatar Nevo, Roberto Barrero, Matthew I. Bellgard, Tianhua He, Xiaohai Tian, Chengdao Li

Grain and Other Field Crops Research Articles

Divergent selection of populations in contrasting environments leads to functional genomic divergence. However, the genomic architecture underlying heterogeneous genomic differentiation remains poorly understood. Here, we de novo assembled two high-quality wild barley (Hordeum spontaneum K. Koch) genomes and examined genomic differentiation and gene expression patterns under abiotic stress in two populations. These two populations had a shared ancestry and originated in close geographic proximity but experienced different selective pressures due to their contrasting micro-environments. We identified structural variants that may have played significant roles in affecting genes potentially associated with well-differentiated phenotypes such as flowering time and drought response between …


Critical P, K And S Concentrations In Soil And Shoot Samples For Optimal Tedera Productivity And Nodulation, Daniel Real, Richard G. Bennett, Nazanin K. Nazeri, David M. Weaver Jun 2022

Critical P, K And S Concentrations In Soil And Shoot Samples For Optimal Tedera Productivity And Nodulation, Daniel Real, Richard G. Bennett, Nazanin K. Nazeri, David M. Weaver

Grain and Other Field Crops Research Articles

Tedera is a forage legume that can provide out-of-season green feed in Mediterranean climates. To date, growers have had no comprehensive soil nutrition guidelines to optimise tedera production. We undertook field and glasshouse studies to understand tedera’s macronutrient requirements. Three field experiments were sown with tedera cv. Lanza® at Cunderdin, Dandaragan and Three Springs in Western Australia. These experiments evaluated seven levels of phosphorus (P) (0–30 kg ha−1) and potassium (K) (0–80 kg ha−1) and two combined treatments with P and K. Glasshouse pot experiments were conducted using tedera cultivars Lanza® and Palma and lucerne cultivar SARDI Grazer. Ten concentrations …


Fine-Mapping And Characterisation Of Genes On Barley (Hordeum Vulgare) Chromosome 2h For Salinity Stress Tolerance During Germination, Edward Mwando, Yong Han, Tefera Angessa, Xiao-Qi Zhang, Chengdao Li Jun 2022

Fine-Mapping And Characterisation Of Genes On Barley (Hordeum Vulgare) Chromosome 2h For Salinity Stress Tolerance During Germination, Edward Mwando, Yong Han, Tefera Angessa, Xiao-Qi Zhang, Chengdao Li

Grain and Other Field Crops Research Articles

Salinity causes a detrimental impact on plant growth, particularly when the stress occurs during germination and early development stages. Barley is one of the most salt-tolerant crops; previously we mapped two quantitative trait loci (QTL) for salinity tolerance during germination on the short arm of chromosome 2H using a CM72/Gairdner doubled haploid (DH) population. Here, we narrowed down the major QTL to a region of 0.341 or 0.439 Mb containing 9 or 24 candidate genes belonging to 6 or 20 functional gene families according to barley reference genomes v1 and v3 respectively, using two DH populations of CM72/Gairdner and Skiff/CM72, …


Oestrogenic Subterranean Clover Guide. Identification And Remediation, Kevin J. Foster, Megan H. Ryan, Daniel R. Kidd May 2022

Oestrogenic Subterranean Clover Guide. Identification And Remediation, Kevin J. Foster, Megan H. Ryan, Daniel R. Kidd

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Subterranean clover (subclover, Trifolium subterraneum L.) is an annual pasture legume that provides high quality feed for livestock. It is estimated to be sown over 30 million hectares across southern Australia, with cultivars developed for the low, medium and high rainfall zones. Unfortunately, some older cultivars contain high levels of phytoestrogen which can affect the reproductive system of sheep and cause other health issues. This became a significant issue for sheep producers from the 1940s as new land was cleared and sheep grazed subclover-dominant pastures. There was a collection of livestock issues observed and these were grouped together under the …


Estimating Effects Of Radiation Frost On Wheat Using A Field-Based Frost Control Treatment To Stop Freezing Damage, Brenton A. Leske, Ben Biddulph Mar 2022

Estimating Effects Of Radiation Frost On Wheat Using A Field-Based Frost Control Treatment To Stop Freezing Damage, Brenton A. Leske, Ben Biddulph

Grain and Other Field Crops Research Articles

Crop phenotyping experiments have long struggled to have a reliable control treatment that excludes frost and associated freezing damage to plants. Previous attempts used a barrier, such as a removable shelter or cloth to exclude frost. However, these methods were labour intensive and varied in their effectiveness. An automated diesel heater was used to protect field plots of wheat (Triticum aestivum L.) from frost damage. In 2018 and 2019 there were 22 and 33 radiation frost events from July to October at the field site. The heater maintained canopy air temperature above freezing (>0 °C) for the duration of …


Non-Escaping Frost Tolerant Qtl Linked Genetic Loci At Reproductive Stage In Six Wheat Dh Populations, Jingjuan Zhang, Md Shahidul Islam, Yun Zhao, Masood Anwar, Zaid Alhabbar, Maoyun She, Rongchang Yang, Angela Juhasz, Guixiang Tang, Jiansheng Cheng, Darshan L. Sharma, Nathan Height, Ben Biddulph, Hang Liu, Yanjie Jiang, Shengnan Zhai, Xin Hu, Junkang Rong, Yingquan Zhang, Yebo Qin, Qier Liu, Zitong Yu, Yujuan Zhang, Sadegh Balotf, Mirza Dowla, Sonia Afrin, Nandita Roy, Md Resad Mallik, Md Atik Us Saieed, Shanjida Rahman, Nigarin Sultana, Sarah Al-Sheikh Ahmed, Chris Florides, Kefei Chen, Meigin Lu, Jorge E. Mayer, Wujun Ma Feb 2022

Non-Escaping Frost Tolerant Qtl Linked Genetic Loci At Reproductive Stage In Six Wheat Dh Populations, Jingjuan Zhang, Md Shahidul Islam, Yun Zhao, Masood Anwar, Zaid Alhabbar, Maoyun She, Rongchang Yang, Angela Juhasz, Guixiang Tang, Jiansheng Cheng, Darshan L. Sharma, Nathan Height, Ben Biddulph, Hang Liu, Yanjie Jiang, Shengnan Zhai, Xin Hu, Junkang Rong, Yingquan Zhang, Yebo Qin, Qier Liu, Zitong Yu, Yujuan Zhang, Sadegh Balotf, Mirza Dowla, Sonia Afrin, Nandita Roy, Md Resad Mallik, Md Atik Us Saieed, Shanjida Rahman, Nigarin Sultana, Sarah Al-Sheikh Ahmed, Chris Florides, Kefei Chen, Meigin Lu, Jorge E. Mayer, Wujun Ma

Grain and Other Field Crops Research Articles

Reproductive stage frost poses a major constraint for wheat production in countries such as Australia. However, little progress has been made in identifying key genes to overcome the constraint. In the present study, a severe frost event hit two large-scale field trials consisting of six doubled haploid (DH) wheat populations at reproductive stage (young microspore stage) in Western Australia, leading to the identification of 30 robust frost QTL on 17 chromosomes. The major 18 QTL with the phenotype variation over 9.5% were located on 13 chromosomes including 2A, 2B, 2D, 3A, 4A, 4B, 4D, 5A, 5D, 6D, 7A, 7B and …


Phenotypic Evaluation And Genetic Analysis Of Seedling Emergence In A Global Collection Of Wheat Genotypes (Triticum Aestivum L.) Under Limited Water Availability, Michael G. Francki, Grantley Stainer, Esther Walker, Gregory J. Rebetzke, Katia T. Stefanova, Robert J. French Dec 2021

Phenotypic Evaluation And Genetic Analysis Of Seedling Emergence In A Global Collection Of Wheat Genotypes (Triticum Aestivum L.) Under Limited Water Availability, Michael G. Francki, Grantley Stainer, Esther Walker, Gregory J. Rebetzke, Katia T. Stefanova, Robert J. French

Grain and Other Field Crops Research Articles

The challenge in establishing an early-sown wheat crop in southern Australia is the need for consistently high seedling emergence when sowing deep in subsoil moisture ( > 10 cm) or into dry top-soil (4 cm). However, the latter is strongly reliant on a minimum soil water availability to ensure successful seedling emergence. This study aimed to: (1) evaluate 233 Australian and selected international wheat genotypes for consistently high seedling emergence under limited soil water availability when sown in 4 cm of top-soil in field and glasshouse (GH) studies; (2) ascertain genetic loci associated with phenotypic variation using a genome-wide association study …


2022 Western Australian Crop Sowing Guide, B. Shackley, B. Paynter, J. Bucat, M. Seymour, S. Power Oct 2021

2022 Western Australian Crop Sowing Guide, B. Shackley, B. Paynter, J. Bucat, M. Seymour, S. Power

Bulletins 4000 -

Welcome to the 2022 edition of the Crop Sowing Guide for WA, which introduces 27 new variety releases: three wheat, nine barley (three recently accredited for malt and six currently under malt evaluation), twelve canola, two field pea and a lentil.

The Crop Sowing Guide for WA has been compiled by officers in the Department of Primary Industries and Regional Development. It provides information to support variety decisions for each of the major crops for the upcoming season.

In this edition, lupin agzones have been reduced from 8 agzones to 6 so that all varieties for each of the major …


Primary Industries Development Research Highlights 2021, Department Of Primary Industries And Regional Development, Tim Scanlon Aug 2021

Primary Industries Development Research Highlights 2021, Department Of Primary Industries And Regional Development, Tim Scanlon

Books & book chapters

The Primary Industries Development Research Highlights 2021 showcases the breadth and depth of the Department of Primary Industries and Regional Development’s research and development activities over the past several years.

Stories featured in Research Highlights 2021 stem from about 60 (of 140) current and recently-completed projects undertaken by the Department of Primary Industries and Regional Development’s (DPIRD) 1100 scientists, technical experts and economists throughout the State.

Explore our Research Highlights 2021.

The publication demonstrates the innovative and applicable research that DPIRD and its collaborators and investment partners deliver to Western Australia.

Download the Research Highlights 2021 here. Alternatively, …


Hybridisation-Based Target Enrichment Of Phenology Genes To Dissect The Genetic Basis Of Yield And Adaptation In Barley, Camilla Beate Hill, Tefera Angessa, Lee-Anne Mcfawn, Debbie Wong, Josquin Tibbits, Xiao-Qi Zhang, Kerrie Forrest, David Moody, Paul Telfer, Sharon Westcott, Dean A. Diepeveen, Yanhao Xu, Cong Tan, Matthew Hayden, Chengdao Li Nov 2018

Hybridisation-Based Target Enrichment Of Phenology Genes To Dissect The Genetic Basis Of Yield And Adaptation In Barley, Camilla Beate Hill, Tefera Angessa, Lee-Anne Mcfawn, Debbie Wong, Josquin Tibbits, Xiao-Qi Zhang, Kerrie Forrest, David Moody, Paul Telfer, Sharon Westcott, Dean A. Diepeveen, Yanhao Xu, Cong Tan, Matthew Hayden, Chengdao Li

Grain and Other Field Crops Research Articles

Barley (Hordeum vulgare L.) is a major cereal grain widely used for livestock feed, brewing malts and human food. Grain yield is the most important breeding target for genetic improvement and largely depends on optimal timing of flowering. Little is known about the allelic diversity of genes that underlie flowering time in domesticated barley, the genetic changes that have occurred during breeding, and their impact on yield and adaptation. Here, we report a comprehensive genomic assessment of a worldwide collection of 895 barley accessions based on the targeted resequencing of phenology genes. A versatile target-capture method was used to …


Nam Gene Allelic Composition And Its Relation To Grain-Filling Duration And Nitrogen Utilisation Efficiency Of Australian Wheat, Zaid Alhabbar, Rongchang Yang, Angela Juhasz, Xin Hu, Maoyun She, Masood Anwar, Nigarin Sultana, Dean Diepeveen, Wujun Ma, Shahidul Islam Oct 2018

Nam Gene Allelic Composition And Its Relation To Grain-Filling Duration And Nitrogen Utilisation Efficiency Of Australian Wheat, Zaid Alhabbar, Rongchang Yang, Angela Juhasz, Xin Hu, Maoyun She, Masood Anwar, Nigarin Sultana, Dean Diepeveen, Wujun Ma, Shahidul Islam

Grain and Other Field Crops Research Articles

Optimising nitrogen fertiliser management in combination with using high nitrogen efficient wheat cultivars is the most effective strategy to maximise productivity in a cost-efficient manner. The present study was designed to investigate the associations between nitrogen utilisation efficiency (NUtE) and the allelic composition of the NAM genes in Australian wheat cultivars. As results, the non-functional NAM-B1 allele was more responsive to the nitrogen levels and increased NUtE significantly, leading to a higher grain yield but reduced grain protein content. Nitrogen application at different developmental stages (mid-tillering, booting, and flowering) did not show significant differences in grain yield and protein content. …