Plant Breeding and Genetics Commons^™

New Semi-Dwarfing Alleles With Increased Coleoptile Length By Gene Editing Of Gibberellin 3-Oxidase 1 Using Crispr-Cas9 In Barley (Hordeum Vulgare L.), Jingye Cheng, Camilla Hill, Yong Han, Tianhua He, Xingguo Ye, Sergey Shabala, Ganggang Guo, Meixue Zhou, Ke Wang, Chengdao Li

Grain and Other Field Crops Research Articles

The green revolution was based on genetic modification of the gibberellin (GA) hormone system with “dwarfing” gene mutations that reduces GA signals, conferring shorter stature, thus enabling plant adaptation to modern farming conditions. Strong GA-related mutants with shorter stature often have reduced coleoptile length, discounting yield gain due to their unsatisfactory seedling emergence under drought conditions. Here we present gibberellin (GA) 3-oxidase1 (GA3ox1) as an alternative semi-dwarfing gene in barley that combines an optimal reduction in plant height without restricting coleoptile and seedling growth. Using large-scale field trials with an extensive collection of barley accessions, we showed that a natural …

Insights Into Early-Stage Inbreeding Depression In Hawaiian Metrosideros, Rosalie Chaleunsouck

Undergraduate Research Symposium Podium Presentations

Inbreeding depression is the reduced biological fitness of offspring resulting from the conception of two genetically related gametes. Selfing, the fusion of gametes from the same individual, is the most extreme form of inbreeding, common in many plant species. Inbreeding depression is most prevalently seen at the seed-development stage (Levin, 1981).

Successful Creation Of Seedless (Sterile) Leucaena Germplasm Developed From Interspecific Hybridisation For Use As Forage, Daniel Real, Clinton K. Revell Dr, Yong Han, Chengdao Li, Marieclaire Castello, C. D. Bailey

Grain and Other Field Crops Research Articles

Context: The legume shrub/tree leucaena (Leucaena leucocephala (Lam.) de Wit.) is highly regarded as a cattle fodder, although it is considered an environmental weed in many parts of Australia.

Aims: We investigated the feasibility of developing a forage variety of triploid leucaena through interspecific hybridisation that is sterile (without seeds) as a strategy to mitigate its weed risk.

Methods: A conventional breeding strategy was used to create triploid hybrids from hand-crosses between tetraploid and diploid species of leucaena. Molecular markers were developed to confirm successful crosses and flow cytometry was used to confirm the ploidy level. The plants are being …

Flowering Habit And Photoperiodic Flowering Response Of Stevia (Stevia Rebaudiana Bertoni) In The Philippines, Kennilyn May Balbin-De Luna, Bong Salazar

The Philippine Agricultural Scientist

Stevia is a perennial shrub commercially cultivated for the sweet-tasting compounds known as steviol glycosides (SVglys). SVglys are extracted from the green leaves, thus maintenance of vegetative growth is critical in stevia production. This study was conducted to describe the flowering habit and response of potted stevia plants to different photoperiods under Philippine conditions. Photoperiodic treatments (10, 11, 12, 13, and 14 h) were provided using light chambers. Stevia has both terminal and axillary flowering habits. It has five white florets enclosed in a capitulum and exhibits an opposite branching pattern. Results showed that stevia is a facultative short-day plant …

Determination Of Some Seed Characteristics Of False Flax (Camelina Sativa L. Crantz) Genotypes Grown Under Semi-Temperate Conditions, İlhan Subaşı, Yusuf Arslan, Tamer Eryigit, Vahdettin Çiftçi, Mahmut Çamlıca

The Philippine Agricultural Scientist

Camelina is an alternative low-input oil plant grown in marginal areas in Turkey. This study aimed to determine some quality characteristics of 39 camellia genotypes in ecological conditions in Bolu, Turkey. The field experiments were carried out in an augmented design with randomized blocks. In this work, some important properties such as thousand seed weight, oil content, fatty acid composition, and protein ratio were investigated. Results showed that mean data for thousand seed weight, oil ratio, and protein ratio were 0.63 – 1.5 g, 26.69 – 39.17%, and 21.81 – 29.81%, respectively. Considering the oil content of the genotypes used …

Micronutrient Concentration Effects On Lettuce Growth And Susceptibility To Pythium, Kalyn M. Helms

Graduate Theses and Dissertations

In hydroponic production waterborne pathogens such as Pythium are ubiquitous and continually threaten a wide range of Controlled Environment Agriculture (CEA) crops in hydroponic production, including but not limited to: lettuce, spinach, basil, arugula, cucumber, tomato, sweet pepper, roses, chrysanthemums, and cannabis (Sutton et al., 2006; Gull, 2002; McGehee and Raudales, 2021; Gillespie, 2020). Despite extensive sanitation measures, disease control in hydroponics is fallible and requires constant surveillance and management to minimize outbreaks (Sutton et al., 2006). A potential disease suppression strategy is to increase micronutrient concentrations within hydroponic systems to naturally strengthen plant defenses against pathogens such as Pythium. …

Arkansas Soybean Research Studies 2021, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading soybean-producing state in the mid-southern United States. Arkansas ranked 11th in soybean production in 2021 compared to the other soybean-producing states in the U.S. The state represented 3.49% of the total U.S. soybean production and 3.49% of the total acres planted in soybean in 2021. The 2021 state soybean average yield was 52.0 bushels per acre, setting a new state record and surpassing the previous yield record of 51.5 bushels per acre set in 2020. The top five soybean-producing counties in 2021 were Mississippi, Phillips, Crittenden, Poinsett, and Arkansas (Table 1). These five counties accounted for …

Characterization Of Algerian Apricots (Prunus Armeniaca) Using Morphological And Pomological Markers, Kaouther Boutiti, Ines Bellil, Douadi Khelifi

Karbala International Journal of Modern Science

The aim of this study was to evaluate the diversity of an Algerian apricot germplasm. This Algerian apricot was characterized by a green-yellow skin, a red ground color, and a light orange flesh color in general. Besides, highly positive and negative significant correlations were revealed between the studied characters. Whereby, the principal component analysis explained 81% of the variability. Fruit, stone and leaves dimensions were the main features that explained evidentially the majority of variability. Moreover, the cluster analysis divided the accessions into two major groups. Thus, Algerian accessions selected in this study may have the potential to be used …

Identification Of Quantitative Trait Loci (Qtls) For Resistance To Bacterial Leaf Streak: Xanthomonas Translucens Using Qtl And Association Mapping In Three Populations Of Soft Red Winter Wheat, Benjamin Tyler Meritt

LSU Doctoral Dissertations

Bacterial leaf streak (BLS) and black chaff, caused by Xanthomonas translucens pv. undulosa (Xtu), can be a very destructive disease of wheat, especially in the warmer, wetter areas of the Southeastern U.S. Yield losses of up to 40 percent have been recorded in some cases in southern wheat growing regions. With no effective agronomic or chemical method of disease control, identification of genetic resistance is seen as a promising solution. Three soft red winter wheat populations (GAWN, ARK-SNP, and AGS 2060- AGS 2035 DH) representative of soft red winter wheat germplasm in the southeastern U.S. developed by …

Genomics And Transcriptomics To Protect Rice (Oryza Sativa L.) From Abiotic Stressors: -Pathways To Achieving Zero Hunger, Mushtaq Ahmad

Center for Plant Science Innovation: Faculty Publications

More over half of the world’s population depends on rice as a major food crop. Rice (Oryza sativa L.) is vulnerable to abiotic challenges including drought, cold, and salinity since it grown in semi-aquatic, tropical, or subtropical settings. Abiotic stress resistance has bred into rice plants since the earliest rice cultivation techniques. Prior to the discovery of the genome, abiotic stressrelated genes were identified using forward genetic methods, and abiotic stress-tolerant lines have developed using traditional breeding methods. Dynamic transcriptome expression represents the degree of gene expression in a specific cell, tissue, or organ of an individual organism at …

2023 Western Australian Crop Sowing Guide, B. Shackley, B. Paynter, J. Bucat, M. Seymour, H. Dhammu

Bulletins 4000 -

Welcome to the 2023 edition of the Crop Sowing Guide for WA, which introduces 31 new variety releases: two wheat, seven barley (one as feed only and six currently under malt evaluation), 15 canola, three hay-only oat and four lentil varieties.

The Crop Sowing Guide for WA has been compiled by officers in the Department of Primary Industries and Regional Development. It provides information to support variety decisions for each of the major crops for the upcoming season.

In this edition, the canola NVT series are now identified by GRDC/NVT as ‘Low-Med Rainfall’ and ‘Med–High Rainfall’ to reflect the environments …

Molecular Pathways Of Wrky Genes In Regulating Plant Salinity Tolerance, Lewis Price, Yong Han, Tefera Angessa, Chengdao Li

Grain and Other Field Crops Research Articles

Salinity is a natural and anthropogenic process that plants overcome using various responses. Salinity imposes a two-phase effect, simplified into the initial osmotic challenges and subsequent salinity-specific ion toxicities from continual exposure to sodium and chloride ions. Plant responses to salinity encompass a complex gene network involving osmotic balance, ion transport, antioxidant response, and hormone signalling pathways typically mediated by transcription factors. One particular transcription factor mega family, WRKY, is a principal regulator of salinity responses. Here, we categorize a collection of known salinity-responding WRKYs and summarize their molecular pathways. WRKYs collectively play a part in regulating osmotic balance, ion …

Variation In Morpho‑Physiological And Metabolic Responses To Low Nitrogen Stress Across The Sorghum Association Panel, Marcin Grzybowski, Mackenzie Zwiener, Mackenzie Zwiener, Hongyu Jin, Nuwan K. Wijewardane, Abbas Atefi, Michael J. Naldrett, Sophie Alvarez, Yufeng Ge, James C. Schnable

Center for Plant Science Innovation: Faculty Publications

Background: Access to biologically available nitrogen is a key constraint on plant growth in both natural and agricultural settings. Variation in tolerance to nitrogen deficit stress and productivity in nitrogen limited conditions exists both within and between plant species. However, our understanding of changes in different phenotypes under long term low nitrogen stress and their impact on important agronomic traits, such as yield, is still limited.

Results: Here we quantified variation in the metabolic, physiological, and morphological responses of a sorghum association panel assembled to represent global genetic diversity to long term, nitrogen deficit stress and the relationship …

Arkansas Wheat Performance Tests 2021-2022, J. F. Carlin, R. B. Morgan, R. D. Bond, D. E. Moon

Arkansas Agricultural Experiment Station Research Series

Wheat variety performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station, Department of Crop, Soil, and Environmental Sciences. The tests provide information to companies developing varieties and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for small-grain producers. The tests are conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Pine Tree Research Station near Colt, and the Rohwer Research Station near Rohwer. Specific location …

Summaries Of Arkansas Cotton Research 2021, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

While the basic growth and development of the cotton plant have not changed significantly in recent history, the business of cotton production is ever-changing. The last two years have seen us plant a crop just about as late as we thought possible, yet extended favorable conditions at season's end have been our salvation, helping to lead us to record yields. The economic environment over the last few years has been such that farmers need to produce record or near-record yields to advance. Unfortunately, production levels at the state yield average barely cover out-of-pocket expenses.

Great uncertainties exist for the upcoming …

Adaptation Of Quinoa (Chenopodium Quinoa Willd.) To Australian Environments, Richard Snowball, Harmohinder S. Dhammu, Mario Francesco D'Antuono, David Troldahl, Ian Biggs, Callen Thompson, Mark Warmington, Amanda Pearce, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Quinoa is being evaluated in cropping systems in many countries outside of its natural range of South America. Very few attempts have been made by farmers or researchers to grow or evaluate quinoa under Australian environments. Given the growing popularity of quinoa with consumers, new commercial opportunities for farmers and international interest in the crop, it was timely to undertake a comprehensive evaluation of the potential of quinoa in Australia. Two advanced selections and nine germplasm lines (six of Chilean and three of Bolivian origin) identified in an earlier project were tested in 23 field trials at 14 locations on …

Genome Architecture And Diverged Selection Shaping Pattern Of Genomic Differentiation In Wild Barley, Wenying Zhang, Cong Tan, Haifei Hu, Rui Pan, Yuhui Xiao, Kai Ouyang, Yong Jia, Xiao-Qi Zhang, Camilla Hill, Penghao Wang, Brett Chapman, Yong Han, Gaofeng Zhou, Le Xu, Yanhao Xu, Tefera Angessa, Hao Luo, Sharon Westcott, Darshan Sharma, Eviatar Nevo, Roberto Barrero, Matthew I. Bellgard, Tianhua He, Xiaohai Tian, Chengdao Li

Grain and Other Field Crops Research Articles

Divergent selection of populations in contrasting environments leads to functional genomic divergence. However, the genomic architecture underlying heterogeneous genomic differentiation remains poorly understood. Here, we de novo assembled two high-quality wild barley (Hordeum spontaneum K. Koch) genomes and examined genomic differentiation and gene expression patterns under abiotic stress in two populations. These two populations had a shared ancestry and originated in close geographic proximity but experienced different selective pressures due to their contrasting micro-environments. We identified structural variants that may have played significant roles in affecting genes potentially associated with well-differentiated phenotypes such as flowering time and drought response between …

Analysis Effect Of Shade Level On The Physiological And Anatomical Characteristics Of Hybrid Phalaenopsis Orchid At The Acclimatization Stage, Ade Arisma Fauziah, Nintya Setiari, Endang Saptiningsih

Jurnal Kultivasi

Production of hybrid Phalaenopsis seedlings is generally applied by in vitro culture techniques. The final stage of in vitro culture is acclimatization. The acclimatization stage is crucial because the plantlets must adapt to the ex-vitro environment. Shade supports plantlet growth during the early stages of acclimatization. This study aims to determine the effect of shade level on the physiological and anatomical characteristics of the Phalaenopsis hybrid orchid at the acclimatization stage. The research used Phalaenopsis plantlet hybrid and shading level. Plantlets were shaded at 40%, 55%, and 70%. Parameters measured included: photosynthetic pigment content, number and area of leaves, number …

Karakter Agronomi Beberapa Jagung Hibrida Padjadjaran Dan Hubungannya Dengan Hasil Di Dataran Medium, Yuyun Yuwariah, Desri Nursyahbani Putri, Dedi Ruswandi, Fiky Yulianto Wicaksono, Dhany Esperanza

Jurnal Kultivasi

Budidaya tanaman jagung dengan menggunakan varietas unggul baru dapat memenuhi kebutuhan jagung, baik sebagai pangan maupun pakan dan industri. Karakter agronomi yang dimiliki oleh varietas unggul menjadi kebutuhan evaluasi perakitan kualitas benih. Penelitian ini bertujuan untuk mengetahui karakter agronomi pada jagung hibrida Padjadjaran secara umum maupun masing-masing Jagung Hibrida Padjadjaran yang menentukan hasil. Penelitian ini dilakukan di Sanggar Penelitian, Latihan dan Pengembangan Pertanian (SPLPP) Universitas Padjadjaran di Desa Arjasari, Kecamatan Arjasari, Kabupaten Bandung yang dilakukan sejak 20 Februari hingga 20 Juli 2020. Percobaan ini menggunakan Rancangan Acak Kelompok dan analisis regresi linear berganda dengan 12 perlakuan dan 3 ulangan. Perlakuan …

Effect Of Paclobutrazol On Growth And Root Morphology Of 12 Crossed Stevia In Vitro, Suseno Amien, Qurrota Aini, Noladhi Wicaksana

Jurnal Kultivasi

Stevia (Stevia rebaudiana Bertoni) is a low-calorie alternative sweetener. The superior varieties of Stevia in Indonesia are limited. Availability of seeds plays an important role in the Stevia plant breeding program for both the selection stage and the production of high-yielding varieties. Tissue culture is the best method of Stevia propagation, but the low adaptability of plantlets causes death in the acclimatization phase. The growth and root morphology of 12 crossbreed Stevia were evaluated. A Completely Randomized Design was used with factorial consisting of two factors were Stevia genotypes (STG1,7,8,10, SBG3,4,5,7,10, SGB2,3, and SBT11) and the plant growth regulator (PGR) …

Rekayasa Budidaya Dan Penanganan Pascapanen Untuk Meningkatkan Kualitas Teh Indonesia Sebagai Minuman Fungsional Kaya Antioksidan, Intan Ratna Dewi Anjarsari

Jurnal Kultivasi

Teh sebagai minuman fungsional sejalan dengan tema hari keamanan pangan 2021 yang bertema Safe food today for a healthy tomorrow, yang menekankan bahwa produksi dan konsumsi produk pangan yang aman akan memiliki manfaat jangka panjang untuk kesehatan dan ekosistem untuk masa depan. Teh (Camelia sinensis L. (O.) Kuntze ) merupakan minuman yang kaya akan senyawa bioaktif berupa antioksidan yang baik untuk kesehatan. Tidak hanya sebagai penghilang dahaga, tetapi kini teh berkembang sebagai minuman fungsional yang berperan dalam aspek kesehatan. Jawa Barat sebagai produsen teh dengan produksi terbanyak di Indonesia tentunya memiliki potensi yang cukup besar dalam mengembangkan industri teh mulai …

Lentil (Lens Culinaris Medik.) Prebiotic Carbohydrates And Protein Quality: Uncovering Genomic Associations And Developing Rapid Ftir Phenotyping Methods, Nathan Johnson

All Dissertations

Lentil (Lens culinaris Medik.) is a cool-season food legume cultivated around the globe. This pulse crop boasts a rich nutrient profile including high concentrations of prebiotic carbohydrates, protein, essential amino acids, and micronutrients, such as folate, iron, zinc, and selenium. Prebiotic carbohydrates promote a healthy gut microbiome, which, in turn, is associated with reduced risk of numerous pathologies including obesity/overweight, type II diabetes, irritable bowel disease, and colon cancer. Known as “poor man’s meat,” lentil also provides high quality plant-based protein at a low cost. As the world increasingly looks to crops to supplement and replace animal-based protein, lentil …

Crosscutting Understandings Of Fusarium Resistance In Sorghum And Wheat For Development Of Breeding Strategies And Novel Opportunities In Germplasm Improvement, Arlyn Ackerman

All Dissertations

Fusarium causes widespread disease in crops resulting in yield and quality loss across a range of host taxa that results in global incidence of carcinogenic mycotoxins contaminating the world’s food systems. This research sought to crosscut frameworks developed across the Fusarium host crop species of wheat (Triticum aesitivum) and sorghum (Sorghum bicolor [L] Moench) to further understand mycotoxin suppression and resistance. A deeper understanding of the biochemical resistances available in sorghum grain mold (SGM) was built by following research of wheat based upon deoxynivalenol resistance to Fusarium head blight (FHB). Through this research, phenotyping methods for Fusarium damaged kernels …

B.R. Wells Arkansas Rice Research Studies 2021, J. Hardke, X. Sha, N. Bateman

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading rice producer in the United States. The state represents 47.5% of total U.S. rice production and 47.8% of the total acres planted to rice in 2021. Rice cultural practices vary across the state and across the U.S. However, these practices are also dynamic and continue to evolve in response to changing political, environmental, and economic times. This survey was initiated in 2002 to monitor and record changes in the way Arkansas rice producers approach their livelihood. The survey was conducted by polling county extension agents in each of the counties in Arkansas that produce rice. Questions …

Genetic Dissection And Qtl Pyramiding To Enhance Salinity Tolerance In Rice (Oryza Sativa L.), Sandeep Chapagain

LSU Doctoral Dissertations

Salinity is emerging as a major threat to rice production worldwide due to climate change and anthropogenic sources. We performed three different population development approaches using exotic salt tolerant donors in US rice backgrounds to identify the salt tolerant QTLs/genes and tolerant breeding lines. First approach involved development of a biparental introgression lines (ILs) population by utilizing a salinity tolerant donor ‘TCCP’ in an elite rice cultivar ‘Cheniere’ background and mapping of QTLs at germination, seedling, and reproductive stages A total of seven QTLs for five germination traits, 16 QTLs for seven seedling stage traits, and 30 QTLs for ten …

Genetic And Biochemical Investigation Of Seed Fatty Acid Accumulation In Arabidopsis, Chinedu Charles Nwafor, Delin Li, Ping Qin, Long Li, Wei Zhang, Yuanwei Zhou, Jingjing Xu, Yongtai Yin, Jianbo Cao, Limin He, Fu Xiang, Chao Liu, Liang Guo, Yongming Zhou, Edgar B. Cahoon, Chunyu Zhang

Center for Plant Science Innovation: Faculty Publications

As a vegetable oil, consisting principally of triacylglycerols, is the major storage form of photosynthetically-fixed carbon in oilseeds which are of significant agricultural and industrial value. Photosynthesis in chlorophyll-containing green seeds, along with photosynthesis in leaves and other green organs, generates ATP and reductant (NADPH and NADH) needed for seed fatty acid production. However, contribution of seed photosynthesis to fatty acid accumulation in seeds have not been well-defined. Here, we report the contribution of seed-photosynthesis to fatty acid production by probing segregating green (photosynthetically-competent) and non-green or yellow (photosynthetically-non-competent) seeds in siliques of an Arabidopsis chlorophyll synthase mutant. Using this …

Insect Pest Management With Sex Pheromone Precursors From Engineered Oilseed Plants, Hong-Lei Wang, Bao-Jian Ding, Jian-Qing Dai, Tara J. Nazarenus, Rafael Borges, Agenor Mafra-Neto, Edgar B. Cahoon, Per Hofvander, Sten Stymne, Christer Löfstedt

Center for Plant Science Innovation: Faculty Publications

Pheromones have become an environmentally friendly alternative to conventional insecticides for pest control. Most current pheromone-based pest control products target lepidopteran pests of high-value crops, as today’s manufacturing processes cannot yet produce pheromones at low enough costs to enable their use for lower-value crops, especially commodity crops. Camelina sativa seeds genetically modified to express (Z)-11-hexadecenoic acid, a sex pheromone precursor of several moth species, provided the oil from which the precursor was isolated, purified and transformed into the final pheromone. Trap lures containing this pheromone were then assessed for their capacity to manage moth pests in the field. Plant-derived pheromone …

Linkage Analysis And Residual Heterozygotes Derived Near Isogenic Lines Reveals A Novel Protein Quantitative Trait Loci From A Glycine Soja Accession, Yia Yang, Thang C. La, Jason D. Gillman, Zhen Lyu, Trupti Joshi, Mariola Usovsky, Qijian Song, Andrew Scaboo

Biomedical Sciences

Modern soybean [Glycine max (L.) Merr] cultivars have low overall genetic variation due to repeated bottleneck events that arose during domestication and from selection strategies typical of many soybean breeding programs. In both public and private soybean breeding programs, the introgression of wild soybean (Glycine soja Siebold and Zucc.) alleles is a viable option to increase genetic diversity and identify new sources for traits of value. The objectives of our study were to examine the genetic architecture responsible for seed protein and oil using a recombinant inbred line (RIL) population derived from hybridizing a G. max …

Critical P, K And S Concentrations In Soil And Shoot Samples For Optimal Tedera Productivity And Nodulation, Daniel Real, Richard G. Bennett, Nazanin K. Nazeri, David M. Weaver

Grain and Other Field Crops Research Articles

Tedera is a forage legume that can provide out-of-season green feed in Mediterranean climates. To date, growers have had no comprehensive soil nutrition guidelines to optimise tedera production. We undertook field and glasshouse studies to understand tedera’s macronutrient requirements. Three field experiments were sown with tedera cv. Lanza® at Cunderdin, Dandaragan and Three Springs in Western Australia. These experiments evaluated seven levels of phosphorus (P) (0–30 kg ha−1) and potassium (K) (0–80 kg ha−1) and two combined treatments with P and K. Glasshouse pot experiments were conducted using tedera cultivars Lanza® and Palma and lucerne cultivar SARDI Grazer. Ten concentrations …

Fine-Mapping And Characterisation Of Genes On Barley (Hordeum Vulgare) Chromosome 2h For Salinity Stress Tolerance During Germination, Edward Mwando, Yong Han, Tefera Angessa, Xiao-Qi Zhang, Chengdao Li

Grain and Other Field Crops Research Articles

Salinity causes a detrimental impact on plant growth, particularly when the stress occurs during germination and early development stages. Barley is one of the most salt-tolerant crops; previously we mapped two quantitative trait loci (QTL) for salinity tolerance during germination on the short arm of chromosome 2H using a CM72/Gairdner doubled haploid (DH) population. Here, we narrowed down the major QTL to a region of 0.341 or 0.439 Mb containing 9 or 24 candidate genes belonging to 6 or 20 functional gene families according to barley reference genomes v1 and v3 respectively, using two DH populations of CM72/Gairdner and Skiff/CM72, …