Plant Breeding and Genetics Commons^™

The Effect Of Thidiazuron Treatment On The Growth Of Vanda Limbata Blume In Vivo, Novika Ditasya Putri, Nintya Setiari, Yulita Nurchayati, Sri Widodo Agung Suedy

Makara Journal of Science

Vanda limbata is one of the most popular ornamental orchids. Currently, its growth is slow, making it necessary to improve its cultivation techniques. One of the possible ways to achieve this is to use plant growth regulator (PGR) treatment. Thidiazuron (TDZ) is a cytokinin used to increase the growth of V. limbata. This study aimed to determine the effect of TDZ concentration on the growth rate of V. limbata. The study involved cultivating V. limbata on planting media and spraying it with TDZ at various concentrations. This research uses a CRD (Completely Randomized Design) single-factor, which is TDZ concentration at …

Maize Hybrids’ Genetic Variability Based On Qualitative And Quantitative Traits, Slamet Bambang Priyanto, Herawati, Suwarti, Ayyub Ar Rahman, Nining Nurini Andayani, Abdul Fattah, Muhammad Azrai

Jurnal Kultivasi

Genetic variability was a prerequisite to doing a plant breeding program. A broad genetic variability allows plant breeders to select a desired genotype. This research aims to assess the maize hybrid's genetic variability based on qualitative and quantitative traits. This research was conducted in the Bone district, south Sulawesi, from November 2022 to March 2023. Fifteen maize hybrids were arranged in a randomized complete block design with three replications. The variables observed are qualitative and quantitative traits. Principal component (PCA) and cluster analyses assessed the genetic variability. The result indicated that based on a loading factor greater than 0.70, the …

Diversity Of Gibberellin 2-Oxidase Genes In The Barley Genome Offers Opportunities For Genetic Improvement, Jingye Cheng, Yong Jia, Camilla Hill, Tianhua He, Ke Wang, Ganggang Guo, Sergey Shabala, Meixue Zhou, Yong Han, Chengdao Li

Grain and Other Field Crops Research Articles

Introduction

Gibberellin (GA) is a vital phytohormone in regulating plant growth and development. During the “Green Revolution”, modification of GA-related genes created semi-dwarfing phenotype in cereal crops but adversely affected grain weight. Gibberellin 2-oxidases (GA2oxs) in barley act as key catabolic enzymes in deactivating GA, but their functions are still less known.

Objectives

This study investigates the physiological function of two HvGA2oxgenes in barley and identifies novel semi-dwarf alleles with minimum impacts on other agronomic traits.

Methods

Virus-induced gene silencing and CRISPR/Cas9 technology were used to manipulate gene expression of HvGA2ox9 and HvGA2ox8ain barley and RNA-seq …

Investigate The Possibility Of Producing Healthier, Safer Peanuts Through Molecular Breeding And Novel Fungal Pathogen Management Strategies, Tariq Alam

All Dissertations

Peanut allergy, a critical health concern with high morbidity and mortality rates, is a leading cause of fatal anaphylactic reactions in the United States. Managing peanut allergy is challenging due to the prevalence of peanuts in processed foods and the risk of contamination. This dissertation evaluates the U.S. peanut mini-core collection for four major immunogenic proteins, Ara h1, Ara h2, Ara h3, and Ara h6, using gel electrophoresis, enzyme-linked immunosorbent assay, liquid chromatography, and mass spectrometry. The study identified 26 genotypes with reduced concentrations of allergenic proteins. Genome-wide association studies (GWAS) of protein traits revealed 10 significant SNPs and identified …

Morphological And Physiological Changes Of Katuray (Sesbania Grandiflora) During Seed Maturation [Research Note], Alangelico San Pascual, Lucille Elna De Guzman, Maurice Gravidez, Elijah Estante

The Philippine Agricultural Scientist

Katuray (Sesbania grandiflora), whose flowers are consumed as steamed vegetables, is an important green manure crop in the Philippines. Understanding different processes leading to seed maturity can aid in determining S. grandiflora’s seed quality, preventing premature harvesting for seed increase, ensuring efficient propagation and production of seedlings, and breeding new varieties of this crop. Multiple flowers were tagged, and three to five pods were collected five days after anthesis (DAA) and at a five-day interval thereafter until 100% germination was achieved. Seed size, moisture content (MC), dry weight, germination percentage, and embryo development were observed and recorded. Seed length was …

Root Plasticity Of Selected Corn (Zea Mays L.) Varieties Grown For Food And Forage In Response To Fertigroe®-N And Urea, Vanica Apostol, Edna Aguilar, Lilia Fernando, Lourdes Edano Ma., Tonette Laude, Eureka Teresa Ocampo

The Philippine Agricultural Scientist

Root plasticity, the capacity of a corn root system to modify its growth and development in response to varying environmental conditions, is a crucial adaptability trait for optimizing water and nutrient uptake, especially in a challenging environments. Understanding root plasticity provides insights into the pattern basis of crop adaptation, contributing to the development of improved corn varieties and agricultural practices that optimize the production of both ear shoots and forage. This study assessed root traits and other morphological-physiological responses of three corn varieties (NK8840, IPB Var 6, and Pearl Sweet) grown for food (ear shoots) and forage (baby corn fodder) …

Comparison Of Statistical And Machine Learning Genomic Prediction Methods In Plant Breeding: Case Studies In Maize And Soybean, Igor Kuivjogi Fernandes

Graduate Theses and Dissertations

Plant breeding is essential to increase genetic gain and food production worldwide. This study was conducted to evaluate new ways to use machine learning (ML) to tackle plant breeding challenges, where two ideas were tested — the first chapter focuses on how to combine genetic and environmental data using ML to improve the prediction of maize grain yield in multi-environment trials, while the second chapter centers on how to couple feature selection of molecular markers with ML to enhance prediction of yield in soybean, and, in both cases, ML approaches were compared to well-established statistical methods greatly adopted by the …

Pangenome And Pantranscriptome As The New Reference For Gene-Family Characterization: A Case Study Of Basic Helix-Loop-Helix (Bhlh) Genes In Barley, Cen Tong, Yong Jia, Haifei Hu, Zhanghui Zeng, Brett Chapman, Chengdao Li

Grain and Other Field Crops Research Articles

Genome-wide identification and comparative gene-family analyses have commonly been performed to investigate species-specific evolution linked to various traits and molecular pathways. However, most previous studies have been limited to gene screening in a single reference genome, failing to account for the gene presence/absence variations (gPAVs) in a species. Here, we propose an innovative pangenome-based approach for gene-family analyses based on orthologous gene groups (OGGs). Using the basic helix-loop-helix (bHLH) transcription factor family in barley as an example, we identified 161–176 bHLHs in 20 barley genomes, which can be classified into 201 OGGs. These 201 OGGs were further classified into 140 …

Evaluation Of Cotton Advanced Lines/Varieties For Genetic Diversity And Correlation Studies Of Cotton Leaf Curl Virus Disease With Yield Contributing Traits In Cotton, Nouman Bilal, Wajid Nazeer, Abdul Latif Tipu, Shoaib Liaqat, Muhammad Sarwar, Ansar Ali, Khadim Hussain, Maria Basheer

Journal of Bioresource Management

Plant genetic diversity aids in the creation of new varieties that are more resistant to pests and unfavorable climatic conditions. In order to discover varied parents and assess their cross-performance, the current study was carried out. The findings showed a substantial positive connection (0.81) between green bolls, plant height (0.57), and open bolls, as well as a significant positive correlation (0.52) between open bolls. On the other hand, a highly significant negative connection was discovered between the number of open bolls (-0.59), number of green bolls (-0.72), plant height (-0.78), and major stem nodes (-0.70) for the cotton leaf curl …

Effects Of Soil Type, Irrigation Method, And Biomass Application On Grain Yield And Its Determinants In Irrigated Lowland Rice Of Agusan [Research Note], Jehru C. Magahud

The Philippine Agricultural Scientist

Information about the influence of soil type, irrigation method, and biomass use on rice productivity in Agusan is insufficient, despite their importance in developing or improving irrigated lowland rice production. Hence, this study determined the influence of these factors on grain yield and yield-contributing crop traits. It also explained grain yield based on yield determinants and recommended water management for improving grain yield. The greenhouse experiment was laid out in a three-factor randomized complete block design with 10 replications. The treatments were [a] Butuan sandy loam soil (SLS) and Butuan loam soil (LS); [b] continuously flooded (CF) and controlled irrigation …

Are You Planting Resistant Weeds With Your Crop Seed?, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Key messages

• West Australian (WA) grain growers have significant weed seed contamination in their crop seed.

• WA growers are sowing herbicide resistant weed seed into their cropping paddocks.

• Crop hygiene (harvest, seeding, transporting) is important to minimise spread of weeds.

Harvest is well underway throughout the WA grainbelt, and growers will be harvesting grain and collecting seed for next year’s crop.

However, this crop seed can be contaminated with weed seeds and, if seed cleaning operations are only partially effective, this weed seed may be sown into paddocks with crop seed in the following season.

The Effect Of Various Doses Of Gamma Ray Irradiation On Growth And Chlorophyll Changes Of Three Adlay Genotypes, Warid Ali Qosim, Anas, Suseno Amien, Meddy Rachmadi, Sidik Ramdani, Romi Zamhir Islami

Jurnal Kultivasi

Adlay has great potential to be developed into an alternative food source in Indonesia because it contains carbohydrates and high fat. The aim was to effect of various doses of gamma-ray irradiation on growth,chlorophyll changes, and determination of lethal dose (LD50) three adlay genotypes in M1 generation. This study was conducted by using the experimental quantitative method without design. The material used consists of three adlay genotypes, including genotypes #28, #37, and #38. The seeds were treated with 0 Gy (control), 100 Gy, 200 Gy, 300 Gy, 400 Gy, and 500 Gy doses of gamma-ray irradiation by the Research Center …

Genetic Parameters Of Yield Component And Yield In M1 Rice (Oryza Sativa L.) Generation Irradiated With Gamma-Ray, Agus Riyanto, Eka Oktaviani, Nur Kholida Wulansari, Totok Agung Dwi Haryanto

Jurnal Kultivasi

High-yielding varieties are the primary determinant of the success of increasing Indonesia's rice production. Plant breeding for new high-yielding varieties is possible through mutation induction and selecting desired traits. The effectiveness and efficiency of selection require comprehensive genetic parameters. This study aimed to study the genetic diversity, heritability, genetic advance, and relationship between yield components and yield in the M1 generation of mutant rice irradiated with gamma ray. This research used a factorial randomized complete block design, involving gamma irradiation doses of 100 Gy, 150 Gy, and 200 Gy, as the first factor; and the second factor was the rice …

Enhancing Crop Resilience: Physiological And Omics Approaches For Drought And Heat Stress Tolerance In Soybean And Peanut, Jyoti Prasad Kakati

All Dissertations

Soybean and peanut are two major crops globally grown for oil and protein. Climate change-associated factors like drought and increased temperature (heat stress) challenge the global production of soybeans and peanuts. Though the United States is the second-largest producer of soybean and the fourth-largest producer of peanuts, it still faces production constraints associated with drought and heat stresses. In the present study, we have examined the responses of soybean and peanut plants to drought and heat stresses during germination and reproductive phases. We conducted field and controlled environmental studies to elucidate the impact of drought stress on germination and seedling …

B.R. Wells Arkansas Rice Research Studies 2023, J. Hardke, X. Sha, N. Bateman

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading rice producer in the United States. The state represents 49.0% of total U.S. rice production and 49.6% of the total acres planted to rice in 2023. Rice cultural practices vary across the state and across the U.S. However, these practices are also dynamic and continue to evolve in response to changing political, environmental, and economic times. This survey was initiated in 2002 to monitor and record changes in the way Arkansas rice producers approach their livelihood. The survey was conducted by polling county extension agents in each of the counties in Arkansas that produce rice. Questions …

Leveraging The Genomic Tools To Explore The Molecular Basis Of Salinity Tolerance In Rice And Soybean, Rajat Pruthi

LSU Doctoral Dissertations

Salinity stress significantly challenges agricultural productivity, particularly affecting major crops like rice and soybean. This research investigates the genetic basis of salt tolerance in both crops through high-resolution genetic mapping, genome-wide association studies (GWAS), and RNA-Seq analysis. In rice, we examined quantitative trait loci (QTLs) associated with salt tolerance at the seedling and flowering stages using a population of advanced backcrossed introgression lines (ILs). A genetic map, constructed with 14,230 polymorphic SNP markers, identified distinct QTLs and candidate genes for salt tolerance at both stages, suggesting differential genetic controls. Notable genes such as OsHAK13 and OsCYP21-4 were upregulated under salt …

Transcriptome-Wide Genetic Variations In The Legume Genus Leucaena For Fingerprinting And Breeding, Yong Han, Alexander Abair, Julian Van Der Zanden, Madhugiri Nageswara-Rao, Saipriyaa Purushotham Vasan, Roopali Bhoite, Marieclaire Castello, Donovan Bailey, Clinton K. Revell Dr, Chengdao Li, Daniel Real

Grain and Other Field Crops Research Articles

Leucaena is a versatile legume shrub/tree used as tropical livestock forage and in timber industries, but it is considered a high environmental weed risk due to its prolific seed production and broad environmental adaptation. Interspecific crossings between Leucaena species have been used to create non-flowering or sterile triploids that can display reduced weediness and other desirable traits for broad use in forest and agricultural settings. However, assessing the success of the hybridisation process before evaluating the sterility of putative hybrids in the target environment is advisable. Here, RNA sequencing was used to develop breeding markers for hybrid parental identification in …

New Insights On Hybridization In Potamogeton Floridanus (The Florida Pondweed), Kaitlyn R. Sampson

Graduate Theses and Dissertations (2019 - present)

Freshwater ecosystems are some of the most important and highest threatened habitats in the world, and aquatic plants play an important, but often-overlooked, role in maintaining them. Potamogeton is a diverse and ecologically important aquatic plant genus well known for taxonomic difficulty and rampant hybridization. lbis study aimed to 1) test the hypothesis that Potamogeton jloridanus (Florida pondweed) is a hybrid between P. oakesianus and P. pulcher, and 2) to investigate correlations in ecological conditions for the focal species. This study revealed the discovery of a new population of P. jloridanus in Big Coldwater Creek in Santa Rosa Co., FL, …

Evaluating Past Progress And Assessing Prediction Breeding Strategies For Sustained Genetic Gains In The Louisiana Sugarcane Variety Development Program, Brayden A. Blanchard

LSU Doctoral Dissertations

The aim of this dissertation is to outline important considerations for the Louisiana Sugarcane Variety Development Program (LSVDP) as it pertains to historical progress, impact, goal setting, and new strategies for continued genetic gains. Industry progress was evaluated with robust regression models to quantify rates of productivity gains. Over the last 50 years, statistically significant productivity gains were identified in sucrose content (45%), cane yield (32.2%), and sugar yield (93%) while pairwise comparisons of decades showed that progress was incremental rather than rapid and sustained once achieved. The decade from 1990-1999 was identified as the only decade with a significant …

Isolation Of Gametophytic Selection And Selective Embryo Abortion In Mimulus Guttatus, Desmond C. Willson

University Honors Theses

A lack of genomic studies examining gametophytic selection (GS) and selective embryo abortion (SEA)--which occur during the reproduction of angiosperms--leaves questions regarding the adaptive and evolutionary effects of these processes. Analyzing deviations from Mendelian segregation offers an avenue for identifying loci targeted by GS and SEA, and their contributions to purging of genetic load. However, other selective processes such as meiotic drive and cytonuclear interactions, as well as pollen and ovule abortion, can cause distortion. To distinguish the effects of GS and SEA from other causes of distortion, we will perform reciprocal crosses between highly homozygous and highly heterozygous individuals …

Genetic Diversity Analysis Of Greater Yam (Dioscorea Alata L.) Collections Using Tuber Morphology And Simple Sequence Repeats (Ssr) Markers, Junelyn Ravelo, Lara Jaaziel Batalon, Antonio Lalusin

The Philippine Agricultural Scientist

The diversity of 148 greater yam (Dioscorea alata L.) accessions from the collections of the National Plant Genetics Resources Laboratory (NPGRL), Visayas State University (VSU), and the Institute of Crop Science - University of the Philippines Los Baños (ICROPS-UPLB) was evaluated using 54 Dioscorea-based SSR markers. Out of 54 SSR markers, polymorphic bands were amplified in 50 SSR markers with Polymorphic Information Content (PIC) values ranging from 0.65 to 0.89. Subsequent cluster analysis generated nine distinct clusters with a Jaccard’s distance index of 0.85, implying 85% dissimilarity among the greater yam accessions. Each formed cluster from the analysis comprised …

Genetic Approaches To Improving Coffee Resistance Against Diseases And Pests In The Philippines [Review Paper], Nick Rainier Santos, Ernelea Cao, Daisy May Santos

The Philippine Agricultural Scientist

There is a huge demand for coffee in the Philippines but local supply has a long way to go at catching up. One way to aid local farmers is to develop coffee varieties with combined desirable traits including natural resistance against various diseases and insect pests. This can increase product output and reduce costs on chemical control that can, in turn, potentially increase their profits. This paper reviews and gives an overview of the genetic research on coffee toward improving plant defense. The most prominent diseases and pests plaguing plantations worldwide are noted. From studies using classical tools such as …

Identification Of Downy Mildew Resistance In Wild Arugula And Evaluation Of Downy Mildew Causing Pathogens And Management Practices, Reilly B. Blair

Master's Theses

Downy mildew has been a potentially devastating constraint to crop production within the Brassicaceae family worldwide (CABI, 2022) and on the Central Coast of California (Smukler, et al, 2008; Koike, et al. 2007). Downy mildew is a foliar disease, caused by the pathogens in the Hyaloperonospora genus on plants within the Brassicaceae family (Goker, et al, 2009). Downy mildew is a disease that is common in region of the world with Coastal climates that provide humidity and moderate temperatures (Sarahan, et al, 2017). Crops within the Brassicaceae family maintain a predominant role in agricultural systems worldwide (Cartea, et al, 2011) …

Summaries Of Arkansas Cotton Research 2023, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

With current production costs and cotton prices, either record or near-record yields are needed for profitable cotton production in Arkansas. As usual, price volatility in 2023 added another level of difficulty in the quest for being profitable. Cotton futures traded between 80 and 90 cents per pound in 2023, with brief times below 80 cents and above 90 cents. In April, cotton futures dropped below 80 cents per pound, the lowest level in almost four months. This was due to an increase in certificated stocks, declining demand, and a growing world carryover. The average cotton price received by U.S. growers …

Reaction-Diffusions System Simulated On Irregular Shapes And Surfaces Model Petal Spot Patterns In Monkeyflower Hybrids, Emily Simmons, Arielle M. Cooley, Joshua R. Puzey, Gregory D. Conradi Smith

Biology and Medicine Through Mathematics Conference

No abstract provided.

The De-Domestication Of Ornithopus Sativus Brot. To Develop Cultivars With Physical Dormancy (Hardseed), Robert J. Harrison, Bradley J. Nutt, Ronald J. Yates, Belinda F. Hackney, John G. Howieson

Grain and Other Field Crops Research Articles

Ornithopus sativus Brot. (French serradella) is a forage legume that is well adapted to acidic coarse textured soils (sands) which are characterized by poor nutrition and an inability to retain water. During the process of domestication of O. sativus, there was an unintentional loss of seed physical dormancy (PY) thus compromising its self-regeneration after a cropping interval. Through mass screening of seed, we identified for the first time that heritable sources of PY exist in three populations of O. sativus. This rare genetic material was then incorporated into suitable genetic backgrounds of differing maturity through targeted hybridization. We demonstrated …

Transcriptomic Profile Of Armillaria Root Rot Fungi Affecting The U.S. Stone Fruit Industry, Jared Weaver

All Theses

Armillaria Root Rot (ARR) is a significant replant disease threatening the future of the United States stone fruit industry. Economic loss in South Carolina peach orchards is estimated to be $8M annually due to approximately 3-4% of annual tree deaths caused by ARR on replant sites. Management solutions are insufficient and can at best extend the life of the orchards on replant sites. The only solution to solving the ARR problem, apart from abandoning the replant site for peach production, is planting orchards on ARR-resistant rootstock. Significant progress has been made towards providing growers with genetically resistant rootstock, however, for …

The Effect Of Fruit Maturation Stage On Seed Germination Of Iaa9-3 And Iaa9-5 Tomato Mutants, Elfa Muhammad Ihsan Al Aufa, Syariful Mubarok, Anne Nuraini, Rahmat Budiarto, Nurmalita Waluyo, Hiroshi Ezura

Jurnal Kultivasi

One of the internal factors that play an important role in seed quality is the level of the phytohormone auxin. Efforts to increase auxin in seeds include developing new varieties with increased indigenous auxin, such as those found in mutant tomatoes from Micro-Tom, namely iaa9-3 and iaa9-5. This research was conducted to determine the germination response of iaa9-3 and iaa9-5 mutant tomato seeds at different levels of the fruit maturation stage. The research was carried out at the Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University, and the Seed Testing Laboratory of the Center for Standard Testing of Vegetable Plant …

Post-Translational Regulation Of A Bidomain Glycerol-3-Phosphate Dehydrogenase Catalyzing Glycerol Synthesis Under Salinity Stress In Chlamydomonas Reinhardtii, Itzela Cruz-Powell, Binita Subedi, Yeongho Kim, Daniela Morales-Sánchez, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty Publications

Core chlorophytes possess glycerol-3-phosphate dehydrogenases (GPDs) with an unusual bidomain structure, consisting of a glycerol-3-phosphate phosphatase (GPP) domain fused to canonical GPD domains. These plastid-localized enzymes have been implicated in stress responses, being required for the synthesis of glycerol under high salinity and triacylglycerols under nutrient deprivation. However, their regulation under varying environmental conditions is poorly understood. C. reinhardtii transgenic strains expressing constitutively bidomain GPD2 did not accumulate glycerol or triacylglycerols in the absence of any environmental stress. Although the glycerol contents of both wild type and transgenic strains increased significantly upon exposure to high salinity, cycloheximide, an inhibitor of …

Enhancement Of Applied Rice Breeding Programs: Exploring Factors Impacting Genomic Selection Accuracy And Discovery Of A Novel Gene For Grain Shape, Maria G. Montiel

LSU Doctoral Dissertations

Plant breeding is a crucial tool in addressing global challenges such as climate change, population growth, and the need for more resilient crops. Rice serves as a staple food for a significant portion of the global population and has served as a model crop for genetic and genomic studies. Genomic selection (GS) has become valuable tool in plant breeding, allowing breeders to predict breeding lines’ performance based on their genotypes. The training (TS) set is a central component of genomic selection and the optimization of the training set is crucial for efficient implementation of genomic selection. The first two objectives …