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Articles 3361 - 3390 of 7020

Full-Text Articles in Pharmacology, Toxicology and Environmental Health

Environmental Influences On Rates Of Osteo- And Rheumatoid Arthritis, Azad Abdulrahman Mohammed Jan 2019

Environmental Influences On Rates Of Osteo- And Rheumatoid Arthritis, Azad Abdulrahman Mohammed

Legacy Theses & Dissertations (2009 - 2024)

Abstract

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Climate Change And Salmonella, New York State, Kevin Taylor Welch Jan 2019

Climate Change And Salmonella, New York State, Kevin Taylor Welch

Legacy Theses & Dissertations (2009 - 2024)

A study of climate change and Salmonella was conducted, serving as the evidence-based results for action to mitigate adverse Salmonella outcomes for the population of New York State (NYS), and to facilitate dissemination of findings in regard to validated approaches for broader use to mitigate climate change and adverse communicable disease outcomes in a variety of other settings. This study bolsters understanding of dynamic exposures and outcomes for improved and targeted public health approaches to localized climate change and communicable disease outcomes. The approach was developed and refined through continuously validated and calibrated methodology for the enhancement of disease control …

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Epitranscriptomic Writer Systems And Codon Bias Regulate The Response To Environmental Stress, Andrea Leonardi Jan 2019

Epitranscriptomic Writer Systems And Codon Bias Regulate The Response To Environmental Stress, Andrea Leonardi

Legacy Theses & Dissertations (2009 - 2024)

Epitranscriptomic marks in the form of enzyme-catalyzed modifications to RNA nucleosides can be important regulators of translation and play integral roles in the response to stress. Dynamic changes in tRNA modification status can regulate the translation of stress response proteins whose transcripts have distinct codon biases. The epitranscriptomic writer Alkylation repair homolog 8 (ALKBH8) plays a crucial part in the translation of codon biased transcripts, as it regulates stop codon recoding, which is a specialized form of translation used to generate selenoproteins. ALKBH8 modifies the wobble uridine of selenocysteine tRNA (tRNASec) to promote the decoding of an internal UGA codon …

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Accumulation And Spatial Distribution Of Lead And Other Trace Elements In Keratinized Tissues : Investigations Based On Analytical Atomic Spectrometry, Mina W. Tehrani Jan 2019

Accumulation And Spatial Distribution Of Lead And Other Trace Elements In Keratinized Tissues : Investigations Based On Analytical Atomic Spectrometry, Mina W. Tehrani

Legacy Theses & Dissertations (2009 - 2024)

Human biomonitoring for toxic metals such as lead (Pb) provides information about absorbed dose from all exposure routes, and is critical for identifying exposed individuals and assessing health risks. Blood Pb and bone Pb are the primary validated biomarkers for assessing short-term and historical Pb exposures, respectively, while non-traditional matrices, including keratinized tissues, can provide complementary exposure information. Hard keratinized tissues – including hair, nails, hooves, and horns – grow incrementally, potentially preserving chemical exposure histories over a span of days to years. Analytical limitations and poor interlaboratory reproducibility, however, have led to debate around the use of human nails …

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Current And Future Environmental And Human Health Impacts Associated With Biofuel/Biofuel Feedstock Production, Eun Kyung Lee Jan 2019

Current And Future Environmental And Human Health Impacts Associated With Biofuel/Biofuel Feedstock Production, Eun Kyung Lee

Legacy Theses & Dissertations (2009 - 2024)

Background: The demand for biobased products, such as food, fuel, and chemicals, has been continuously increasing. Meanwhile, agricultural production, serving as the primary stage of biobased products, is one of the largest contributors to greenhouse gas (GHG) emissions and nutrient releases. Although assessing spatially and temporally explicit environmental releases and impacts are required to inform a sustainable trajectory for agricultural production, such analyses are largely lacking due to data unavailability and feasibility of data analysis. Moreover, our current understanding of health impacts associated with biofuel production are lacking due to the limited assessment of health impacts resulted from biofuels’ entire …

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The Independent And Combined Effects Of Hypoxia And Tributyltin On The Eastern Oyster, Crassostrea Virginica, Ann Fairly Barnett Jan 2019

The Independent And Combined Effects Of Hypoxia And Tributyltin On The Eastern Oyster, Crassostrea Virginica, Ann Fairly Barnett

Electronic Theses and Dissertations

Oysters are vital to the health of estuarine ecosystems and provide critical ecosystem services, such as water quality improvement, nursery habitat for commercially important fisheries, and shoreline stabilization. Despite their ecological and economic importance, oyster reefs are exposed to many stressors, and oyster populations in Mississippi have declined dramatically in recent years. The present study focuses on the effects of hypoxia and tributyltin (TBT) on the Eastern oyster, Crassostrea virginica. Hypoxic events are increasingly comin coastal ecosystems worldwide, and TBT is a legacy toxicant once used as an antifoulant. The effects of these stressors were assessed on adult oysters deployed …

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Exploration Of Sonic Hedgehog Gene Expression In Fathead Minnows (Pimephales Promelas), Brooke Renee Greiner Jan 2019

Exploration Of Sonic Hedgehog Gene Expression In Fathead Minnows (Pimephales Promelas), Brooke Renee Greiner

Undergraduate Honors Theses

Pollutants, as a result of wastewater treatments, have been shown to have negative impacts on aquatic ecosystems. To better understand the possible consequences caused by effluents on ecosystems, it is important to examine ecotoxicology data. One of the most commonly used species for water quality testing is the fathead minnow, Pimephales promelas. Ecotoxicology can then be taken one step further to understand the effects of pollutants on a molecular level. Previous research had identified effluents as causes for abnormal minnow fin morphology. In order to collect additional data on development, tanks with fathead minnows were placed at the Charleston …

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Do Humans Possess The Capability To Regenerate?, Chasha Wuensch Jan 2019

Do Humans Possess The Capability To Regenerate?, Chasha Wuensch

The Science Journal of the Lander College of Arts and Sciences

Urodele amphibians, including newts and salamanders, are amongst the most commonly studied research models for regeneration. The ability to regenerate, however, is not limited to amphibians, and the regenerative process has been observed in mammals as well. This paper discusses methods by which amphibians and mammals regenerate to lend insights into human regenerative mechanisms and regenerative potential. A focus is placed on the urodele and murine digit tip models, both of which share critical regenerative stages including wound healing, histolysis, and blastema formation. Formation of the blastema proved to be a crucial process necessary for regeneration, and is responsible for …

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Degeneration Of Rods And Cones In Retinitis Pigmentosa, Rachel Stern Jan 2019

Degeneration Of Rods And Cones In Retinitis Pigmentosa, Rachel Stern

The Science Journal of the Lander College of Arts and Sciences

Retinitis Pigmentosa, most commonly characterized by night blindness and loss of peripheral vision, is a rare genetically inherited group of diseases affecting the retina of the eye. It is estimated that 1 in 4000 people in the USA are affected by some form of the disease. Retinitis Pigmentosa (RP), is caused by a mutation or change in one or more of 55 genes. There are many causes to this disease as RP presents with many different symptoms and biological effects on the eye. These are then grouped together because they share a common result, deterioration of vision. Presently, there is …

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Energy Drinks: Cardiovascular Effects And The Specific Components Responsible, Malka B. Gelbfish Jan 2019

Energy Drinks: Cardiovascular Effects And The Specific Components Responsible, Malka B. Gelbfish

The Science Journal of the Lander College of Arts and Sciences

Energy drink usage as a stimulant is on the rise among adolescents and young adults. While these drinks have positive energizing effects, they pose significant health concerns. This paper examines the adverse cardiovascular effects of energy drinks and the components that could possibly be responsible. Analysis was conducted by reviewing and comparing many studies available in this area of research. Studies of energy drinks in general and energy drinks versus drinks containing caffeine alone were analyzed. Additionally, this review investigated studies of the specific ingredients in energy drinks such as caffeine, taurine, guarana, and sugar. This review found that energy …

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Pharmacokinetic Study Of Novel Anthraquinone Analogues, Yuhan Guo Jan 2019

Pharmacokinetic Study Of Novel Anthraquinone Analogues, Yuhan Guo

Electronic Theses and Dissertations

Small molecules functioning as negative regulators of MDM2 have been considered as promising anti-cancer agents. A series of novel anthraquinone (AQ) analogues upregulate p53, a well-known tumor suppressor, by inactivating its antagonist protein MDM2. The novel AQ compounds synthesized through the modifications on the rhein scaffold were reported to have successfully arrested tumor growth. To have a better understanding of their underlying metabolism in vivo, pharmacokinetic (PK) studies were performed for the AQ compounds. A selective, accurate and reproducible assay methodology for PK study was set up for each AQ compound. The stabilities of AQ compounds were then evaluated …

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Detection Of Adsorbed Chlordecone On Microplastics In Marine Sediments In Guadeloupe: A Preliminary Study, Fidji Sandre, Charlotte R. Dromard, Karyn Le Menach, Yolande Bouchon-Navaro, Sébastien Cordonnier, Nathalie Tapie, Hélène Budzinski, Claude Bouchon Jan 2019

Detection Of Adsorbed Chlordecone On Microplastics In Marine Sediments In Guadeloupe: A Preliminary Study, Fidji Sandre, Charlotte R. Dromard, Karyn Le Menach, Yolande Bouchon-Navaro, Sébastien Cordonnier, Nathalie Tapie, Hélène Budzinski, Claude Bouchon

Gulf and Caribbean Research

Plastic pollution in the oceans is recognized as a worldwide problem. Since the 1950s, the production of plastics has been increasing and the first reports of microplastics (particles < 500 μm) in the marine environment began to appear in the 1970s. These particles represent a growing environmental problem due to their dispersion in seawater and marine organisms. Additionally, microparticles in general can adsorb pollutants that will then become bioavailable to organisms by being desorbed during digestion, which could be an important pathway for the contamination of organisms. In Guadeloupe and Martinique, an organochlorine pesticide called “chlordecone” was used from 1972 to 1993 in banana plantations and this very persistent pollutant contaminates soils, rivers, and coastal marine areas and accumulates in marine foodwebs. To examine these issues, we had two goals: 1) to assess the contamination of marine sediments by microplastics surrounding Guadeloupe; and 2) to determine the ability of microplastics to adsorb chlordecone, as has been demonstrated for other organochlorine pollutants. To do so, marine sediments were collected in triplicate from 12 sites in coral reef environments around the island. Microplastics from each sample were then enumerated by size, color and shape under a binocular microscope. The results indicate that microplastics are found in all the studied sites and that their distribution could be linked to marine currents or proximity to areas of significant human activities (port activities, agglomeration, etc.). Finally, our preliminary results indicated that chlordecone could be adsorbed onto microplastics, with a concentration ranging from 0.00036—0.00173 µg/µg of microfilter.

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Island Invasion: The Silent Crisis In Hawaii, Sophia Janssen Jan 2019

Island Invasion: The Silent Crisis In Hawaii, Sophia Janssen

Pomona Senior Theses

Keeping out invasive species may, upon first review, seem like a trivial environmental cry from ecologists and deep environmentalists; a belated wish to return to an undeveloped world where nature was pristine. However invasive species create problems that impact all of us and can have far more severe consequences than changing a stunning landscape. These problems are heightened in islands like Hawaii, where the fragile ecosystems have developed over centuries of evolution and adaptation. The introduction of a disease-carrying mosquito can put the people of Hawaii at risk to many vector-born illnesses and create an epidemic, taking human life. The …

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Efficacy And Tolerability Of Atomoxetine Use For Patients With Autism Spectrum Disorders And Attention-Deficit/Hyperactivity Disorder (Adhd) Symptoms: A Systematic Review And Meta-Analysis, Angie El-Said Jan 2019

Efficacy And Tolerability Of Atomoxetine Use For Patients With Autism Spectrum Disorders And Attention-Deficit/Hyperactivity Disorder (Adhd) Symptoms: A Systematic Review And Meta-Analysis, Angie El-Said

Honors Undergraduate Theses

Introduction: Patients with autism spectrum disorders (ASD) and attention-deficit/hyperactivity disorder (ADHD) show more symptoms of ADHD. Since there are more adverse events caused by psychostimulants compared to non-psychostimulants, the use of a non-psychostimulant such as atomoxetine might prove more beneficial for younger patients and/or those with comorbid ADHD. Objective: The aim of this thesis is to determine the efficacy and tolerability of atomoxetine in ASD patients presenting with ADHD, by examining (a) differences in ADHD symptoms for participants receiving atomoxetine versus those receiving placebos, and (b) risk differences in adverse events between these participants. Methods: An electronic search of both …

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Medication Adherence For Foster Children From The Perspective Of Foster Care Providers, Odette Boyd Jan 2019

Medication Adherence For Foster Children From The Perspective Of Foster Care Providers, Odette Boyd

Walden Dissertations and Doctoral Studies

In 2015, over 427,000 children were in foster care and the largest population were in California's system. Of those children, more than 9,400 were prescribed psychotropic medications. Increases of psychotropic medication use have led to investigations and findings of medication oversights in foster care. Medication oversights included medication nonadherence, which was linked to an increase of problematic behaviors in foster children. The purpose of this phenomenological study was to explore the issues of medication adherence for foster care providers who care for foster children of mild to chronic health concerns who were prescribed medications and experienced multiple placements. By utilizing …

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Development Of Mithramycin Analogues With Improved Efficacy And Reduced Toxicity For Treatment Of Ets-Dependent Tumors In Ewing Sarcoma And Prostate Cancer, Joseph Michael Eckenrode Jan 2019

Development Of Mithramycin Analogues With Improved Efficacy And Reduced Toxicity For Treatment Of Ets-Dependent Tumors In Ewing Sarcoma And Prostate Cancer, Joseph Michael Eckenrode

Theses and Dissertations--Pharmacy

Introduction: Genetic rearrangements in Ewing sarcoma, prostate, and leukemia cells result in activation of oncogenic ETS transcription factor fusions. Mithramycin (MTM) has been identified as an inhibitor of EWS-FLI1 transcription factor, a gene fusion product responsible for oncogenesis in Ewing sarcoma. Despite preclinical success, a phase I/II clinical trial testing MTM therapy in refractory Ewing sarcoma was terminated. Liver and blood toxicities resulted in dose de-escalation and sub-therapeutic exposures. However, the promise of selectively targeting oncogenic ETS transcription factors like EWS-FLI1 prompted us to undertake the discovery of more selective, less toxic analogues of MTM. MTM is a potent inhibitor …

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Toward An Enzyme-Coupled, Bioorthogonal Platform For Methyltransferases: Probing The Specificity Of Methionine Adenosyltransferases, Tyler D. Huber Jan 2019

Toward An Enzyme-Coupled, Bioorthogonal Platform For Methyltransferases: Probing The Specificity Of Methionine Adenosyltransferases, Tyler D. Huber

Theses and Dissertations--Pharmacy

Methyl group transfer from S-adenosyl-l-methionine (AdoMet) to various substrates including DNA, proteins, and natural products (NPs), is accomplished by methyltransferases (MTs). Analogs of AdoMet, bearing an alternative S-alkyl group can be exploited, in the context of an array of wild-type MT-catalyzed reactions, to differentially alkylate DNA, proteins, and NPs. This technology provides a means to elucidate MT targets by the MT-mediated installation of chemoselective handles from AdoMet analogs to biologically relevant molecules and affords researchers a fresh route to diversify NP scaffolds by permitting the differential alkylation of chemical sites vulnerable to NP MTs that are unreactive to …

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Pyocyanin, A Virulence Factor Produced By Sepsis-Causing Pseudomonas Aeruginosa, Promotes Adipose Wasting And Cachexia, Nika Larian Jan 2019

Pyocyanin, A Virulence Factor Produced By Sepsis-Causing Pseudomonas Aeruginosa, Promotes Adipose Wasting And Cachexia, Nika Larian

Theses and Dissertations--Pharmacology and Nutritional Sciences

Sepsis is a leading cause of death among critically ill patients that results in metabolic alterations including hypercatabolism, lipoatrophy, and muscle wasting, contributing to the development of cachexia. Septic cachexia is associated with loss of body weight, fat mass, and lean mass and dysregulated immune function. There are currently no efficacious treatment strategies for septic cachexia, and nutritional interventions have limited success in preventing hypercatabolic wasting. Pyocyanin is a virulence factor produced by sepsis-causing Pseudomonas aeruginosa that has been shown to activate the aryl hydrocarbon receptor (AhR), increase inflammation, and produce reactive oxygen species. Thus, pyocyanin represents a novel mechanistic …

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Azithromycin Therapy Reduces Cardiac Inflammation And Mitigates Adverse Cardiac Remodeling After Myocardial Infarction, Ahmed Hamish Neamah Al-Darraji Jan 2019

Azithromycin Therapy Reduces Cardiac Inflammation And Mitigates Adverse Cardiac Remodeling After Myocardial Infarction, Ahmed Hamish Neamah Al-Darraji

Theses and Dissertations--Pharmacology and Nutritional Sciences

Introduction: Myocardial infarction (MI) remains the leading cause of morbidity and mortality worldwide. Induced by cardiomyocyte death, MI initiates a prolonged and uncontrolled inflammatory response which impairs the healing process. Immune cells, such as macrophages, play a central role in organizing the early post-MI inflammatory response and the subsequent repair phase. Two activation states of macrophages have been identified with distinct and complementary functions (inflammatory vs. reparatory). This bimodal pattern of macrophage activation is an attractive therapeutic target to favorably resolve post-MI inflammation and enhance recovery. It has been demonstrated that azithromycin (AZM), a commonly used antibiotic with immunomodulatory effects, …

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Exploring The Role Of Insulin Receptor Signaling In Hippocampal Learning And Memory, Neuronal Calcium Dysregulation, And Glucose Metabolism, Hilaree N. Frazier Jan 2019

Exploring The Role Of Insulin Receptor Signaling In Hippocampal Learning And Memory, Neuronal Calcium Dysregulation, And Glucose Metabolism, Hilaree N. Frazier

Theses and Dissertations--Pharmacology and Nutritional Sciences

In the late 90’s, emerging evidence revealed that the brain is insulin-sensitive, highlighted by broad expression of brain-specific insulin receptors and reports of circulating brain insulin. Contemporary literature robustly supports the role of insulin signaling in normal brain function and suggests that insulin-related processes diminish with aging, evidenced by decreased signaling markers, reduced insulin receptor density, and lower levels of insulin transport across the blood-brain barrier. In the context of pathological cognitive decline, clinical trials using intranasal insulin delivery have reported positive outcomes on memory and learning in patients with mild cognitive decline or early-stage Alzheimer’s disease. However, while the …

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Dough Properties And Baking Characteristics Of White Bread, As Affected By Addition Of Raw, Germinated And Toasted Pea Flour, Kim A. Millar, Catherine Barry-Ryan, Roisin Burke, Sinead Mccarthy, Eimear Gallagher Jan 2019

Dough Properties And Baking Characteristics Of White Bread, As Affected By Addition Of Raw, Germinated And Toasted Pea Flour, Kim A. Millar, Catherine Barry-Ryan, Roisin Burke, Sinead Mccarthy, Eimear Gallagher

Articles

Thermal and non-thermal processing may alter the structure and improve the techno-functional properties of pulses and pulse flours, increasing their range of applications in protein-enhanced foods. The effects of germination and toasting of yellow peas (Pisum sativum) on flour and dough characteristics were investigated. Wheat flour was substituted with raw, germinated and toasted pea flour (30%). The resulting bread-baking properties were assessed. Toasting increased dough water absorption and improved dough stability compared with germinated and raw pea flour (p < 0.05). This resulted in bread loaves with comparable specific volume and loaf density to that of a wheat flour control. Significant correlations between dough rheological properties and loaf characteristics were observed. Addition of pea flours increased the protein content of the breads from 8.4% in the control white bread, to 10.1–10.8% (p < 0.001). Toasting demonstrated the potential to improve the techno-functional properties of pea flour. Results highlight the potential application of pea flour in bread-making to increase the protein content.

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The Effects Of Bisphenol F On Embryonic Cardiac Output In Zebrafish, Kyle Monnot Jan 2019

The Effects Of Bisphenol F On Embryonic Cardiac Output In Zebrafish, Kyle Monnot

Williams Honors College, Honors Research Projects

Bisphenol F (BPF) is an analog compound of the xenoestrogen bisphenol A (BPA). BPA utilization has decreased dramatically due to its detrimental effects as an endocrine disrupter. In place of BPA, BPF is growing to be a ubiquitous chemical in epoxy resin manufacturing. Recent research has begun to investigate the possible endocrinal effects of BPF as an analog of BPA. This study was performed to examine the potential effects of BPF exposure on cardiac parameters of zebrafish (Danio rerio) embryos. Embryos were exposed to 50 µg/L BPF for 48 hours, and cardiac parameters (stroke volume, heart rate, and …

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Qualitative And Quantitative Analysis Of Therapeutic Solutions Using Raman And Infrared Spectroscopy, Alaa A. Makki, Franck Bonnier, Renaud Respaud, Fatma Chtara, Ali Tfayli, Clovis Tauber, Dominique Bertrand, Hugh Byrne, Elhadi Mohammed, Igor Chourpa Jan 2019

Qualitative And Quantitative Analysis Of Therapeutic Solutions Using Raman And Infrared Spectroscopy, Alaa A. Makki, Franck Bonnier, Renaud Respaud, Fatma Chtara, Ali Tfayli, Clovis Tauber, Dominique Bertrand, Hugh Byrne, Elhadi Mohammed, Igor Chourpa

Articles

Anticancer drugs are prescribed and administrated to an increasing number of patients on a daily basis. As a consequence, a number of concerns have been raised about the patient health and safety in the case that the drugs administered are not at the required concentration or even worse not the correct ones. Quality control of therapeutic solutions has therefore been extensively implemented in hospital environments, in order to avoid any failure in the intense workflow faced by administering pharmacists. In the present study, infrared (IR) and Raman spectroscopy have been employed for the analysis of 3 commercially available therapeutic solutions …

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Role Of Mcp-1 And Ccr2 In Ethanol-Induced Damage In The Developing Brain, Kai Zhang Jan 2019

Role Of Mcp-1 And Ccr2 In Ethanol-Induced Damage In The Developing Brain, Kai Zhang

Theses and Dissertations--Pharmacology and Nutritional Sciences

Fetal alcohol spectrum disorders (FASD) are caused by alcohol exposure during pregnancy and is the leading cause of mental retardation. Alcohol exposure during development results in the loss of neurons in the developing brain. The underlying molecular mechanisms are unclear and there currently is no cure for FASD. Ethanol-induced neuronal death is accompanied by neuroinflammation. Chemokine monocyte chemoattractant protein 1 (MCP-1) and its receptor C-C chemokine receptor type 2 (CCR2) are critical mediators of neuroinflammation and microglial activation. Using a third trimester equivalent mouse model of ethanol exposure, we found that treatment of Bindarit (MCP-1 synthesis inhibitor) and RS504393 (CCR2 …

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The Effect Of Glucose, Adenosine-5’-Triphosphate, And Adenosine On Glucose Transporter 9 Expression In Mdck Cells, Jamras Kanchanapiboon, Thitima Pengsuparp Jan 2019

The Effect Of Glucose, Adenosine-5’-Triphosphate, And Adenosine On Glucose Transporter 9 Expression In Mdck Cells, Jamras Kanchanapiboon, Thitima Pengsuparp

The Thai Journal of Pharmaceutical Sciences

This study aimed to investigate the effect of glucose, adenosine triphosphate (ATP) and adenosine and their concentration dependence on GLUT9 expression in hyperglycemic conditions. These compounds have been reported to be associated with hyperglycemia i.

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Effects Of Canagliflozin On Renal And Urinary Angiotensin Converting Enzyme 2 (Ace2) And Neprilysin (Nep) In Db/Db Diabetic Mice, Unmesha Hemant Thanekar Jan 2019

Effects Of Canagliflozin On Renal And Urinary Angiotensin Converting Enzyme 2 (Ace2) And Neprilysin (Nep) In Db/Db Diabetic Mice, Unmesha Hemant Thanekar

Browse all Theses and Dissertations

Diabetes is one of the major causes of chronic kidney disease (CKD) which could lead to end-stage renal disease. Angiotensin-II (Ang-II), a major bioactive peptide of renin angiotensin system (RAS) plays a pivotal role in the pathogenesis and progression of diabetic nephropathy. The reno-degenerative effects of Ang-II can be partially counteracted by Ang (1-7). Therefore, Ang (1-7) forming enzymes, angiotensin converting enzyme 2 (ACE2) and neprilysin (NEP), are hypothesized to have reno-protective effects. In our previous studies, we have demonstrated increased renal shedding of ACE2 and NEP in the urine of type-2 diabetic mice which was attenuated by normalizing hyperglycemia …

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Kinetics Of Microvesicle Particle Release In Keratinocytes, Pariksha Thapa Jan 2019

Kinetics Of Microvesicle Particle Release In Keratinocytes, Pariksha Thapa

Browse all Theses and Dissertations

Microvesicle particles (MVPs) are subcellular particles that could be involved in inter-cellular communication because they carry various bioactive substances including cytokines. Previous studies from our lab has shown that that the lipid mediator Platelet-activating Factor (1-alkyl-2-acetyl-glycerophosphocholine; PAF) and ultraviolet B radiation (UVB) enhances the release of MVP in various cell types like primary keratinocytes, epithelial cell lines, and murine skin. We hypothesized that there may be synergistic increases in MVP release after combination of treatment of keratinocytes with UVB and PAF agonist (CPAF)/phorbol ester (PMA). The combination treatment significantly increases MVP and cytokine release at 4 to 8 hours time …

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Identification Of New Metabolic Mutations That Enhance The Cell-Killing Effect Of Hydroxyurea, A Clinically Used Drug With Multiple Implications, Rittu Elsa Samuel Jan 2019

Identification Of New Metabolic Mutations That Enhance The Cell-Killing Effect Of Hydroxyurea, A Clinically Used Drug With Multiple Implications, Rittu Elsa Samuel

Browse all Theses and Dissertations

Cancer is one of the leading causes of death and a worldwide health issue. Intensive studies have been conducted in the past to unearth new anti-cancer agents. One such anti-proliferative drug is hydroxyurea (HU). HU has been used in clinics for ≥ 100 years to treat various neoplastic and non-neoplastic diseases. Although newer agents have been developed, as a WHO-enlisted essential medicine, it remains the staple drug for the management of chronic myeloproliferative disorders and sickle cell anemia. A better understanding of the HU-induced cell death may improve or expand the therapeutic spectrum of this clinically important drug. HU arrests …

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Implementing Mass Spectrometry For The Structural And Compositional Analysis Of Proteins, Mohammad Riaz Jan 2019

Implementing Mass Spectrometry For The Structural And Compositional Analysis Of Proteins, Mohammad Riaz

Electronic Theses and Dissertations

Lectins are sugar-binding proteins that perform various biological functions such as cellular recognition attachments etc. Pulses the dried edible seeds of certain plants are great sources of lectins and a proteomics approach directed at lectins identification would bring substantial improvement in the lectin identification process. Lectins from nine different plant species were analyzed through SDS-PAGE and proteomics analysis. After LC-MS/MS analysis proteins were identified with protein database searches using Uniprot. Functionally uncharacterized proteins were identified with database searches were annotated with the Pfam and NCBI-CCD databases. In-vitro pharmacological screening will be carried out to assess the pharmacological effects of these …

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Two-Dimensional Correlation Analysis Of Raman Microspectroscopy Of Subcellular Interactions Of Drugs In Vitro, Hugh Byrne, Franck Bonnier, Zeineb Farhane Jan 2019

Two-Dimensional Correlation Analysis Of Raman Microspectroscopy Of Subcellular Interactions Of Drugs In Vitro, Hugh Byrne, Franck Bonnier, Zeineb Farhane

Articles

Two-dimensional (2D) correlation analysis is explored to data mine the time evolution of the characteristic Raman microspectroscopic signatures of the subcellular responses of the nucleoli of human lung cancer cells to the uptake of doxorubicin. A simulated dataset of experimental control spectra, perturbed with systematically time-dependent spectral changes, constituted by a short-term response which represents the initial binding of the drug in the nucleolus, followed by a longer term response of the organelle metabolism, is used to validate the analysis protocol. Applying 2D correlation analysis, the in phase, synchronous correlation coefficients are seen to contain contributions of both response profiles, …

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