Food Science Commons^™

Spectroscopic Characterization And Extraction Optimization Of Catechins In Green Tea, Zach J. Mckinley, Masakatsu Watanabe

SACAD: Scholarly Activities

This research initiative immerses students in a multifaceted analytical chemistry investigation of catechins—polyphenolic antioxidants prevalent in green tea—through the application of both classical and modern techniques. The project emphasizes hands-on experience with real-world sample analysis, method development, and critical data interpretation.

Catechins were extracted from various commercial green tea products, including loose leaves, powders, and dietary supplements, by trituration with ethyl acetate followed by solvent partitioning using a methanol and water solution. The resulting extracts were analyzed using UV-Visible spectroscopy to quantify catechin content. Selected samples were further characterized by liquid chromatography–mass spectrometry (LC-MS), confirming both the identity and purity …

Identification Of Covalent Catechin Adducts Of Enoyl-Acp Reductase (Fabi) From E. Coli Using Mass Spectrometry, Francisco G. Atwi, P. M. Joyner, Rustin Ghidari

Seaver College Research And Scholarly Achievement Symposium

Enoyl-ACP reductase (FabI), an enzyme which is inhibited by catechin, is essential for lipid biosynthesis in bacteria (E. coli). Another FabI inhibitor, triclosan, has strong antibacterial effects,1 but overuse can be detrimental to the environment. Flavonoids, a class of dietary compounds, are known to have potent inhibitory effects on FabI and other kinases.2,3,4 Adducts form as a result of the biochemical reaction between flavonoids and cysteine, a modification which can be verified by m/z shifts on the mass spectra.5 Samples of E. coli protein with overexpressed FabI are utilized: the wild type with ordinary primary structure, and mutant type lacking …

The Effect Of Livelihood Capital Accessibility On The Financial Security And Financial Well-Being Of Sheep Farming Households (Survey In Wanaraja Subdistrict, Garut Regency), Suhendarsyah Suhendarsyah, Unang Yunasaf, Sondi Kuswaryan

Jurnal Ilmu Ternak Universitas Padjadjaran

This study analyzed the influence of livelihood capital accessibility on the financial security and financial well-being of sheep-farming households in Wanaraja Subdistrict, Garut Regency. A quantitative approach was used through a survey with organized interviews of 95 sheep farmers. The research variables include livelihood capital accessibility (X), financial security (Z), and financial well-being (Y). The livelihood capital accessibility construct was built as a Higher-Order Construct with five dimensions: human, natural, financial and physical capital. Data analysis used Partial Least Square Structural Equation Modeling (PLS_SEM) with a two-stage approach. The results evince that social capital are the most influential dimensions in …

Reproductive Performance Of Friesian Holstein Crossbred Dairy Cattle After A Foot-And-Mouth Disease Outbreak In Lembang, Indonesia, Hanunghanindito Pamungkas, Septiyani Septiyani, Nurcholidah Solihati

Jurnal Ilmu Ternak Universitas Padjadjaran

Foot-and-mouth disease (FMD) is a highly contagious viral disease that may impair reproductive efficiency in dairy cattle. This study aimed to evaluate changes in the reproductive performance of Friesian Holstein crossbred dairy cows following an FMD outbreak at UPTD BPTSP and HPT Cikole, Lembang, West Java, Indonesia. A retrospective study was conducted using reproductive records collected from 2019 to 2023. The reproductive parameters analyzed were calving interval, service per conception, conception rate, service period, and first mating postpartum. Differences between the pre- and post-FMD periods were assessed using paired sample t-tests. The results showed significant differences in calving interval, service …

Polymorphism Of The Mx Gene In Riau Kampung Chickens, Elfawati Elfawati, Restu Misrianti, Sadarman Sadarman

Jurnal Ilmu Ternak Universitas Padjadjaran

One of the genetic markers for avian influenza is the Mx gene. The purposed of this study was to determine the polymorphism of the Mx gene of kampung chicken in Riau Province. A total of 61 samples from three subpopulations have been genotyped using PCR-RFLP. Allele and genotype frequencies have been calculated using PopGene. The Mx gene was amplified, producing a DNA fragment measuring 299 base pairs. This fragment was digested with the Hpy81 enzyme, which yielded three genotypes: the AA genotype showed by a single 299 bp band; AG showed by 299, 200, and 99 bp bands; and GG …

The Potency Of Inulin Extraction Waste Fermentation From Local Tubers As A Feed Additive, Mohamad Septian, Danes Suhendra, Mikael Sihite, Dyah Saputri

Jurnal Ilmu Ternak Universitas Padjadjaran

This study aims to determine the potential of inulin extraction waste fermentation as a feed additive based on the content of lactic acid, dry matter, pH, and Fleigh points. This research was conducted from March to June 2022 at the Animal Husbandry and Fisheries Laboratory, Tidar University. The experimental design used in this study was a Completely Randomized Design with 4 treatments (fermentation of inulin extraction waste: T1 Dioscorea alata (uwi), T2: ganyong (Dioscorea alata discolor), T3: gadung(Dioscorea hispida), and T4: umbi ungu (Ipomoea batatas). Each treatment was replicated 5 times. The variables …

The Potential Of Bioactive Peptides From Goat Milk Kefir With Antibacterial Activity As An Antibiotic Substitute, Dhea Athillah, Jajang Gumilar, Eka Wulandari

Jurnal Ilmu Ternak Universitas Padjadjaran

This study aims to determine the optimal incubation time for goat milk kefir that produces the highest antibacterial peptides, and to determine the antibacterial activity of purified goat milk kefir. Protein levels were determined using the microassay method. Kefir, which is known to have the best incubation time for producing the highest antibacterial activity when ultrafiltered with a 10 kDa cut-off membrane, was analyzed for its molecular weight using SDS-PAGE. The antibacterial activity was tested against pathogenic bacteria E. coli and S. aureus. The highest soluble protein content was found in kefir incubated for 36 hours, with a soluble …

Comparative Evaluation Of Five Bacterial Strains For The Fermentation Of Camel Milk, Sifatun Nesa Ali

Theses

In this study, the comparative fermentation performance of lactic acid bacteria Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lb. helveticus, Lb. casei, and Lactiplantibacillus plantarum in Camel Milk (CM) and Bovine Milk (BM) was investigated. Fermentations were carried out at 42°C for 48 hours, and was monitored through bacterial growth, titratable acidity, pH evolution, proteolytic activity, microstructural properties, and rheological characteristics. Although comparable bacterial viability was observed in CM and BM, acidification kinetics varied significantly between CM and BM (p < 0.05). Proteolysis was significantly higher in fermented CM than in BM (p < 0.001), with consistently greater o-Phthalaldehyde Assay (OPA) values in CM across all strains during fermentation (p < 0.001). Scanning electron microscopy revealed more porous, loose protein matrices in fermented CM than in BM, which supports the rheological results showing weaker gel networks and lower rheological strength in fermented CM. Among the tested bacteria, Lb. casei demonstrated superior adaptability, enhanced viability, balanced acidification, and favorable rheological properties in both milks. Overall, the results of this thesis demonstrate strain-specific fermentation responses and support the possible suitability of different starter cultures for improving the technological performance of fermented camel milk products.

Start With What They Know: Using Motivational Interviewing To Create A Cooking Class, A Culinary Intervention For Teens (Autistic And Non-Autistic), Gwendolyn S. Tedder

LEND Trainee Presentations

Autistic teens are at greater risk for having heart disease and diabetes as adults. Food particularity, sensory issues, fixed mindset, and anxiety over trying new things can slow down their interest in preparing food for themselves. Nurturing basic cooking skills and gently encouraging trying new foods would provide more nutritional options to teens as they move into adulthood. A cooking class preceded by Motivational Interviewing (MI) developed further interest in preparing food afterward in teens, both autistic and non-autistic, and in trying new foods for autistic teens with food concerns.

Growing South Dakota (Spring 2026), College Of Agriculture, Food, And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 National heritage, statewide research, local impact
[Page] 7 About the South Dakota Agricultural Experiment Stations
[Page] 14 Managing the mooo-ment: How SDSU researchers are looking to improve cow health during the Transition Period
[Page] 18 Discovering strength in science: How education through undergraduate research prepares students for the future
[Page] 20 From India to Brookings: One grad student’s path to make a world of difference in virology
[Page] 22 Solving a billion-dollar problem: Identifying disease-resistent wheat genes
[Page] 24 Unlocking manure’s potential: SDSU leads multistate study on the power of swine manure in growing better crops
[Page] 28 …

Detection And Quantification Of Allergen Cross-Contact In Shared Frying Oil Using Targeted Mass Spectrometry, Francisca Asigri

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Cow’s milk and egg allergies are prevalent IgE-mediated food allergies that pose significant public health concerns worldwide. Although strict avoidance remains the primary management strategy, unintended exposure can still occur through allergen cross-contact, particularly in commercial and industrial food operations that utilize shared frying oils. The objective of this study was to identify and quantify allergen transfer in shared frying oil and assess the robustness of existing targeted mass spectrometry methods (LC-MS/MS) for detecting milk and egg proteins in heated oil matrices.

Heated oil spikes were developed by incorporating non-fat dry milk (NFDM) and whole egg powder (WEP) into oil …

Metabolomics In Precision Medicine: Application And Prospect In Cancer Research, Ming-Shi Shiao

Journal of Food and Drug Analysis

Metabolomics is the most recent and final discipline among the post-genomic, multiomics fields. It encompasses key technologies in systems biology, providing an overview of low-molecular-weight metabolites while examining disturbances to offer pathophysiological explanations. The two key analytical tools in metabolomics are nuclear magnetic resonance spectroscopy and mass spectrometry, which includes gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Metabolomic research relies on a three-stage data generation process: the first stage involves precise analytical chemistry to produce data; the second stage employs multivariate statistical analysis; and the last stage uses big data as a reference for comparison to establish biochemical, metabolic, and …

Mass Spectrometry-Based Metabolomics For Studying Gut Microbiota-Derived Metabolites In Biological Samples, Ya-Ting Lin, Huai-Hsuan Chiu, Chih-Ning Cheng, Ching-Hua Kuo

Journal of Food and Drug Analysis

Gut microbiota produces a wide range of metabolites and plays a critical role in maintaining host health. Dysregulation of these metabolites can influence host metabolism through systemic circulation, contributing to the development of various diseases, including immunological, neurological and cancer-related disorders. Chromatography coupled with mass spectrometry (MS) has emerged as a powerful analytical approach, offering high sensitivity and resolution for studying gut microbiota-related metabolites. This review provides a comprehensive overview of chromatographic MS-based methods applied to the study of the gut microbial metabolome. We summarize strategies for sample collection, storage, and preparation of commonly analyzed sample types, including feces, plasma/serum, …

Special Issue: Analytical Technologies In Metabolomics: Applications And Future Prospects, Sung-Fang Chen

Journal of Food and Drug Analysis

No abstract for this article type.

Fortification Of Flour Cultivated In The Territory Of The Republic Of Uzbekistan With Own Iron And Zinc, Nodira B. Kurbanova, Gulnoza Z. Djakhangirova, Bakhtiyar I. Kurbanov, Dase D. Klava

CHEMISTRY AND CHEMICAL ENGINEERING

The aim of the study is to investigate the regional characteristics of iron and zinc content in wheat and flour produced in Uzbekistan using instrumental neutron activation analysis, and on this basis to provide a scientific justification for effective approaches to flour fortification and the preservation of micronutrients during grain processing. The study analyzed wheat flour produced from grain grown in five regions of Uzbekistan (Khorezm, Surkhandarya, Bukhara, Tashkent, and Fergana). Instrumental Neutron Activation Analysis (INAA) was applied for the first time in Uzbekistan to comprehensively assess the microelement composition of flour. It was found that fortification using natural sources …

Preparation Of Semi-Hard Cacciota Cheese From Local Milk And Analysis Of Its Physicochemical And Microbiological Properties, Shahnoza U. Yuldasheva, Dilshod P. Rakhimov, Dilrabo Q. Maksumova, Shahlo I. Akhmedova, Asaloy U. Narimonova

CHEMISTRY AND CHEMICAL ENGINEERING

Including cheese products in daily diet will give tangible results, enriching body with nutrients rich in high-quality proteins. The aim of the research is to create an Italian semi-hard cheese, caciotta, from local cow's milk. In the experiment, pepsin of the CHY-MAX® Powder Plus NB brand was used. The resulting product contained 1.46 mg/kg zinc (Zn), which is approximately 34 times lower than the permissible limit (50 mg/kg), which is considered a high quality indicator. From a biological safety point of view, no aflatoxin M1 was detected in the cheese (0.0 mg/kg), indicating the absence of mycotoxin contamination of cow's …

Utah Altitude Adjustments For Home Canning, Margie Memmott, Ariel Petersen, Callahan Adams, Andrea Schmutz

All Current Publications

Knowing your altitude is critical for canning. Safe home food preservation depends on adjusting processing times and pressures for altitude. This fact sheet provides a county-by-county and town-by-town listing of elevations across Utah, paired with the boiling-water and pressure canner adjustments recommended by the U.S. Department of Agriculture (USDA) and the National Center for Home Food Preservation. By referencing this resource, Extension educators, food preservation instructors, and home canners can quickly identify the correct processing adjustment for their location.

Nexus Of Food Security Versus Armed Conflict In The Context Of Climate Change In Mali, Moussa Macalou, Youssouf Diabate, Abdoulaye Nientao, Adama B. Coulibaly

Professional Agricultural Workers Journal

The nexus between food security and armed conflict, with particular focus on developing countries, has been analyzed by a few studies in the literature. Every country under armed conflict is obligated to solve related food security issues. Therefore, the overall objective of this study was to analyze the nexus between households’ food security and armed conflicts in the context of climate change in Mali. To achieve this objective, first, the study used an ordinary least squares model to determine the factors influencing households’ food security. Second, it used an ordered logit model to determine the factors influencing armed conflict. Apart …

Impact Of In-Depth Adoption Of System Of Rice Intensification On Food Security Of Smallholder Farm Households In Mali, Moussa Macalou, John Baptist D. Jatoe

Professional Agricultural Workers Journal

The system of rice intensification (SRI) was implemented in Mali to boost rice productivity. The objectives of this paper are to identify the constraints faced by SRI adopters, estimate the food security index, and assess the impact of in-depth adoption of SRI on smallholder farm households’ food security. The study uses Kendall’s ranking to rank the six main constraints identified from the literature. The study uses the Household Food Insecurity Access Scale to estimate households’ food security index. Using primary data collected on rice farm households, the study combines the multivalued treatment effect inverse probability weighted regression adjustment and the …

Evaluation Of Two Varieties Of Okra (Abelmoschus Esculentus (L. Moench) Growth In A Wiregrass Tunnel House And Field, James E. Currington Sr, Victor Khan, Billie Grant, George X. Hunter, Raymon Shange, Cassandra Searight, Edward Sparks, Jeffery Moore, James E. Currington Iii

Professional Agricultural Workers Journal

“Clemson Spineless” and “Jambalaya” okra were grown in a Tunnel House and field to compare yield and other growth parameters. A split-plot design with three replications/treatment was used; the Tunnel House and field were main plots, and varieties split plot. Plant height was significantly taller, and pod harvesting began two weeks earlier in the Tunnel House. Yield showed no significant differences between varieties, and Tunnel House vs. field-produced okra. However, “Clemson Spineless” produced 9% higher pod yield in the Tunnel House than “Jambalaya,” which had 23% higher field yield than “Clemson Spineless.” An enterprise budget analysis showed that Tunnel House …

Exploring The Influence Of Environmental Motivations On Consumer Attitudes And Purchase Intentions Toward Upcycled Foods, Peng Lu, Jennifer Waldeck, Keith Weber, Hualu Zhou

Communication Faculty Articles and Research

Food loss and waste (FLW) present pressing environmental and economic challenges. Upcycling technologies provide a sustainable pathway by transforming food by-products into value-added products; however, consumer acceptance remains a critical barrier. Guided by Self-Determination Theory (SDT), this study investigates how environmental motivations influence consumers’ attitudes and purchase intentions toward milk enriched with upcycled peanut skins. Data were collected from a nationally representative sample of 1488 U.S. adults. Structural equation modeling (SEM) was employed to test hypothesized relationships among motivational constructs, and cluster analysis was used to identify consumer segments based on motivational profiles. The results show that intrinsic motivation and …

Alternative Tree Species For Utah Syrup Production, Abigail Porter, Steven Price, Youping Sun, Darren Mcavoy

All Current Publications

When thinking about producing syrup from trees, most people's minds go straight to maple trees. However, a variety of other species in the Intermountain West can be tapped for sap, including other maple species, birch, sycamore, aspen, and walnut. This fact sheet reviews alternative tree species for syrup production in Utah.

The Nexus Between Perceived And Scientific Weather Patterns And Its Effects On Cereal Crop Production In Manyoni District, Tanzania, Fadhili Lufunyo Ngajilo, Helena Elias Myeya, Evarist Fundisha Swai

Journal of Humanities and Social Sciences

This study examines the nexus between perceived and scientific weather patterns, and its effects on cereal crop production in Manyoni District; and involved 157 participants. Both quantitative and qualitative data were collected through a mixed methods research approach. The data were collected through a household survey, FGDs, in-depth interviews and documentary review methods for triangulation and complementarity purposes. The quantitative data were analysed through descriptive and inferential statistics with IBM SPSS (version 27) and Microsoft Excel Office (version 2007). The results indicate that smallholder farmers perceived that rainfall had been fluctuating with increasing trends, while temperature and wind had been …

Farmers Markets As Community Infrastructure: Evidence From A Three-Year Statewide Data Project In Utah, Jaclyn Pace, Roslynn Mccann, Blue Cook, Steven Price, Lorali Smith, Dianna Parkynn, Joshua Cafferty

Outcomes and Impact Quarterly

Utah farmers markets play a critical role in food access, small business viability, and community connection, yet limited statewide data document their impact. The Utah Farmers Market Network (UFMN) and Utah State University (USU) Extension conducted a mixed-methods evaluation (2023–2025) and found that farmers markets make valuable contributions to the community.

Evaluating The Printability And Digestibility Of 3d-Printed Pea-Sorghum Protein Gels, Sorour Barekat, Ali Ubeyitogullari

Food Science Faculty Publications and Presentations

This study explored the 3D printability and digestibility of pea and sorghum protein gels as novel plant-based bioinks. First, 25 % sorghum protein gel (SPG25) and 15–25 % pea protein gels were prepared, and the effect of extruders’ nozzle size on printability was evaluated. Samples produced at the optimized conditions were then used for analyzing particle size, microstructure, secondary structure, and in vitro digestibility. Pea protein gel at 20 % (w/w) (PPG20) and SPG25 showed ideal rheological properties, including shear-thinning behavior and rapid recovery. Dual-nozzle 3D printing was optimized at a nozzle diameter of 0.52 mm and a speed of …

Development Of Antimicrobial Food Packaging Films Based On Electrospun Polycaprolactone Nanofibers Encapsulating Thyme Oil Nanoemulsions, Mahfuzur Rahman, Abdus Sobhan, Hari Kotturi, Sarder Sadique, Morshed Khandaker

Publications

This study developed and characterized a novel antimicrobial food packaging film fabricated from electrospun polycaprolactone (PCL) nanofibers incorporated with thyme oil nanoemulsions (TONEs). The concentration of TONEs in the film ranged from 2.5 to 15 % (v/v). The physicochemical properties, including pH, viscosity, and water absorption of the film were evaluated, with results showing favorable values (TONEs pH ≈ 5.46, viscosity ≈ 0.26 cP, contact angle ≈ 109-127⁰ and water absorption ≈ 21.47 %). The microstructure of the film was observed using scanning electron microscopy (SEM), which revealed the uniform dispersion of TONEs within the PCL nanofibrous matrix, with …

Exploring Non-Conventional Substrates: The Role Of Hibiscus And Whey In Kombucha Fermentation, Benjamin J. Lee

Master's Theses

This study evaluated the impact of hibiscus and whey as alternative fermentation substrates on the physicochemical, phytochemical, and microbial properties of kombucha. A 2 × 2 full factorial design was used to compare four treatments: black tea + water (BW), black tea + whey (BH), hibiscus + water (HW), and hibiscus + whey (HH). The fermentation solvents (water or whey) were heated to 88 °C, infused with 0.6% (w/v) black tea or hibiscus for 10 minutes, sweetened with 10% (w/v) sucrose, cooled to 22 °C, and inoculated with 20% (v/v) kombucha starter culture. Fermentations were carried out at 22 °C …

Utah Farmers Markets Impact Report: Strengthening Local Food Systems, Economic Resilience, And Community Well-Being, Jaclyn Pace, Steven Price, Roslynn Brain-Mccann, Joshua Cafferty, Blue Cook

All Current Publications

Utah's farmers markets are more than places to shop — they are essential community infrastructure. With more than 60 markets in operation across the state, people rely on their local market for food, leisure, and community connection. From 2023–2025, the Utah Farmers Market Network (UFMN) and Utah State University (USU) Extension led a three-year, mixed-methods evaluation to document the economic and social contributions of farmers markets across the state. Over three market seasons, more than 230,000 customer responses were collected at markets in St. George, Cedar City, Hurricane, Ogden, Murray, Wheeler Farm, Millcreek, Downtown Salt Lake City, and Price — …

Alkaline And Acid Extraction Of An L-Tryptophan–Sunflower Flour Red Colorant: Production, Characterization, And Utilization, Spencer Nguyen, Marissa Wong, Lilian Were Senger

Food Science Faculty Articles and Research

The objective was to produce and characterize a red colorant resulting from interaction of sunflower-derived chlorogenic acid with L-tryptophan/Trp and to evaluate its applications. Yield, intensity, solubility, stability, protein, and metal content of colorants from pH 9 and pH 11 lyophilized sunflower flour- Trp colorants were measured. Red color formation took < 3 h with alkalization and subsequent acidification. The sunflower flour: Trp mixture ratio that produced the greatest redness was 10:2. The highest red pigment was in supernatants and not the precipitates. The red colorant intensity and solubility were highest at neutral pH when compared to acidic pH solutions. The color stability of the new red colorant in icing and refrigerated whole milk matrices, when assessed weekly for a month, showed no color change in refrigerated milk. After 30 min of incubation at 63 °C, pinkness in milk remained unchanged or increased. The pink color, however, faded in icing over time due to exposure to light. The present study established a new natural red colorant developed from sunflower protein-Trp mixtures at alkaline pH, as well as its application in icings and whole milk for commercial strawberry milk products. This colorant could serve as a replacement for the synthetic petroleum-based Red 3.

Electric Pressure Cookers: What You Need To Know, Callahan Adams, Emily Smith

All Current Publications

Electric pressure cookers (EPCs) offer convenience, versatility, and efficiency in everyday cooking. EPCs offer multiple cooking functions beyond pressure cooking, including sautéing, slow cooking, steaming, cooking rice, and preparing yogurt. They are not suitable for pressure canning, as confirmed by multiple Extension research studies and national guidelines. When used properly, EPCs provide a safe and effective way to prepare meals quickly while saving counter and storage space.