Food Science Commons^™

On-Site Sensory Experience Boosts Acceptance Of Cultivated Chicken, Mark Chong, Angela K. Y. Leung, Tricia Marjorie Fernandez

Research Collection Lee Kong Chian School Of Business

This study set out to assess if presenting cultivated chicken in the context of a familiar meal, in a familiar dining setting, would motivate repeat consumption and recommendation. A survey of 107 diners was conducted at Huber's Butchery and Bistro in Singapore – the world's first butchery to serve cultivated meat – from April to June 2023. The findings showed that eating cultivated chicken significantly boosted post-consumption acceptance levels. In addition, cultivated chicken's tastiness may be a more important factor than its integration into a familiar meal or dish in fostering repeat consumption. Implications for the cultivated meat industry, limitations, …

Gaseous Ozone To Improve The Microbial Safety Of Spices, Seeds, And Nuts, Arshpreet Kaur Khattra

Graduate Theses and Dissertations

Due to the increasing number of reported outbreaks of pathogens in low-moisture foods (LMFs), there is a need to investigate novel non-thermal gaseous technologies to increase the microbiological safety of spices, nuts, and seeds without compromising the product quality. The objective of this study was to inactivate Salmonella spp. in different spices, seeds, and nuts using gaseous ozone treatment and evaluate the suitability of Enterococcus faecium as a potential surrogate for Salmonella. Each food product (dried basil leaves, black peppercorn, chia seeds, and walnuts) was separately inoculated with a 5-strain Salmonella and E. faecium cocktail and equilibrated to water activity …

Assessing Impact Of Food Structure On Oral Tribology And In-Vitro Digestion Of Dairy Products, Lamis Ali

All Graduate Theses and Dissertations, Fall 2023 to Present

In this research, we focused on understanding the critical elements impacting consumer experience and the nutritional value of dairy products, specifically their texture, mouthfeel, and protein breakdown in the gastrointestinal tract. Our study aimed to accomplish two main goals. First, we performed analysis of the tribological attributes of various commercially dairy products. The second objective was to investigate the process of disintegration and protein release in selected dairy products.

The study included an assessment of eight dairy products of varied consistencies: solid like cheddar, cheese curd, and parmesan; semi-solid such as cottage cheese, ricotta cheese, and yogurt; and liquid represented …

Application Of Near-Infrared Spectroscopy And Chemometrics For The Rapid Detection Of Insect Protein Adulteration From A Simulated Matrix, Dongdong Ni, Joost L. D. Nelis, Amanda L. Dawson, Nicholas Bourne, Pablo Juliano, Michelle L. Colgrave, Angéla Juhász, Utpal Bose

Research outputs 2022 to 2026

The popularity of insect protein as a food and feed supplement is growing. Protein quality, end use and prices vary considerably between different insect species, which may incentivise insect protein adulteration. Here, near-infrared (NIR) spectroscopy and chemometrics were used to detect the presence of cricket, black soldier fly larvae (BSFL) and mealworm proteins in a simulated complex insect protein mixture. Additionally, BSFL protein powders collected from three commercial sources were investigated to determine whether the NIR-based technology can discriminate the proteins obtained from different companies based on their composition. The proximate analysis suggests compositional protein, fat and chitin differences between …

Moisture Sorption And Quality Characteristics Of Instant Rice, Anna Mays, Griffiths Atungulu

Food Science Undergraduate Honors Theses

Market demand for instant rice--a processed form of rice that is cooked and dehydrated before it is sold--has risen tremendously and become a significant component of the rice industry. Compared to freshly cooked rice, the quality of instant rice (in terms of texture, color, aroma, etc.) is much lower. There exists little information regarding how instant rice’s storage conditions affect its quality. Such information may be elucidated by studying sorption isotherms which describe the storage temperature, relative humidity, and instant rice moisture relationships.

The purpose of this study was to generate moisture sorption isotherms of instant rice for temperatures and …

Cultivating Excellence: A Literature Review On Harnessing The Power Of The Gut Microbiome For Athletic Performance, Maya Katharine Dean

Honors Projects

The interplay between our gut microbiome and health is immense. This literature review analyzes the current research assessing the interplay between gut microbiome and athletic performance. Knowing how to improve gut microbial diversity via nutrition and supplementation can take athletic performance to the next level; namely improvements in immune, mental, and physical health.

Annotation Of Hypothetical Genes In Lactococcus Lactis Ssp. Il403, Jennifer A. Tangires

Student Scholar Showcase

The human gastrointestinal tract (GIT) harnesses various microbial organisms involved in almost all processes of physiological homeostasis, among these are lactic acid bacteria (LAB). These bacteria, almost all of which belong to the order Lactobacillales, are able to produce lactic acid, and play an important role in food preservation because they produce bacteriocins. Bacteriocins are antimicrobial proteins that are used to fight off related bacteria in their environment that are competing for the same resources. This study focuses on a specific LAB strain, Lactococcus lactis ssp. IL1403 where 21.9% of its predicted genes have not yet been assigned a function. …

Foraging Culture In Iceland: Understanding The Transfer Of Plant Knowledge And Personal Views On Foraging, Ella Jane Maurer

Whittier Scholars Program

Foraging is a broad topic, practiced in different locations throughout history. This study looks into the specific practices, values, and views of foraging in Iceland. There is little previous research that has been done, working with Icelanders, and hearing their stories. Through conducting several interviews of local Icelanders with different backgrounds and a brief six-question survey via the Inaturalist application, this study looks into exactly that. By compiling stories from the interview and survey data, themes were highlighted collecting similar and contrasting statements on ways plants are foraged, opinions on Icelandic foraging culture as a whole, and ways knowledge is …

Evaluation Of Yield And Physicochemical Quality Of Pentadesma Butyracea Butter Obtained By Different Traditional Extraction Methods In Ghana, Josephine Akuba Timtey, Francis Alemawor, William Otoo Ellis, Jacob K. Agbenorhevi, Nana Baah Pepra-Ameyaw

Food Science Faculty Articles and Research

Pentadesma butyracea seed butter or fat (PBSB) is a vegetable fat extracted from the seeds of the P. butyracea plant. The butter has potential use in the food, pharmaceutical, and cosmetic industries. The study investigated traditional PBSB processing methods in Ghana and evaluated their effects on yield, physicochemical parameters, and fatty acid composition. Four traditional extraction methods were identified and evaluated, and these methods are direct wet extraction of PBSB from a fresh mixture of the seed paste and water (DEW); wet extraction of PBSB from a 12-hour fermented mixture of the seed paste and water (FWO); direct wet extraction …

Impact Of Fads Genotype On Polyunsaturated Fatty Acid Content In Human Milk Extracellular Vesicles: A Genetic Association Study, John J. Miklavcic, Natalie Paterson, Jennifer Hahn-Holbrook, Laura M. Glynn

Food Science Faculty Articles and Research

Background

Extracellular vesicles in human milk are critical in supporting newborn growth and development. Bioavailability of dietary extracellular vesicles may depend on the composition of membrane lipids. Single-nucleotide polymorphisms (SNPs) in the fatty acid desaturase gene cluster impact the content of long-chain polyunsaturated fatty acids in human milk phospholipids. This study investigated the relation between variation in FADS1 and FADS2 with the content of polyunsaturated fatty acids in extracellular vesicles from human milk.

Methods

Milk was obtained from a cohort of mothers (N = 70) at 2–4 weeks of lactation. SNPs in the FADS gene locus were determined using …

Investigation Of The Growth Of Listeria In Plant-Based Beverages, Klaudia Bartula, Sambou Biagui, Máire Begley, Michael Callanan

Department of Biological Sciences Publications

The objective of the present study was to evaluate whether the content of sugar, protein, fat, or fibre in commercially available and specially formulated plant-based beverages (oat, soya and pea) influences the growth rates of Listeria. Beverages were inoculated with a strain cocktail of Listeria (approximately 1 × 103 CFU/mL), and the data demonstrated that Listeria could proliferate in all tested beverages. Moreover, varying concentrations of naturally occurring or added sugar (0–3.3%), protein (3.3–5%), fat (1.1–3.5%) and added fibre (0–1.5%) did not have a statistically significant (p > 0.05) impact on the growth rates of Listeria in the tested …

Simultaneous Extraction And Quantitative Analysis Of S-Methyl-L-Cysteine Sulfoxide, Sulforaphane And Glucosinolates In Cruciferous Vegetables By Liquid Chromatography Mass Spectrometry, Armaghan Shafaei, Caroline R. Hill, Jonathan M. Hodgson, Lauren C. Blekkenhorst, Mary C. Boyce

Research outputs 2022 to 2026

Sulfur containing compounds including glucosinolates (GLS), sulforaphane (SFN) and S-methyl-L-cysteine sulfoxide (SMCSO) have been proposed to be partly responsible for the beneficial health effects of cruciferous vegetables. As such, greater understanding of their measurements within foods is important to estimate intake in humans and to inform dietary intervention studies. Herein is described a simple and sensitive method for simultaneous analysis of 20 GLS, SFN and SMCSO by liquid chromatography mass spectrometry. Analytes were effectively retained and resolved on an Xbridge C18 column. Detection can be achieved using high resolution or unit resolution mass spectrometry; the latter making the method more …

Desalination As A Source Of Freshwater, Jacob Pensky

Best Integrated Writing

Jacob Pensky's article deals with technology we use to make saltwater drinkable. Drought-stricken coastal communities need desalination plants, especially as Earth's climate warms, but they are expensive and energy-intensive. This article describes ways to reduce the environmental and monetary costs.

Effect Of Harvest Season On The Physical Properties, Fatty Acid Composition, And Volatile Compounds Of Roasted Cacao Beans, Amornrat Chumthong, Nuttarisa Limjumrern, Chutikarn Saensano, Pornthep Teerawattanapong, Aekkaraj Nualla-Ong, Adirek Rugkong, Rawee Chiarawipa

Makara Journal of Science

The prevailing climate is an important factor affecting the development of the physical quality and flavor of cacao (Theobroma cacao L.) beans. The influence of harvest seasons on the physical, chemical, and flavor characteristics of cacao beans from Nakhon Si Thammarat Province, southern Thailand, was assessed. Among cacao beans, those harvested in the rainy season (R) had the highest fresh and dry weights and the lowest bean count per 100 g (87.00 ± 2.83). Although the overall fat content remained relatively constant across harvest seasons, cacao beans harvested in R exhibited a significantly higher fatty acid (C16:0, C18:1, and …

Assessment Of The Genotoxic Effect Of Pesticide (Profenophos And Cypermethrin) On Allium Cepa L. Through Comet Assay, Ali Hasnian, Muhammad Rizwan, Hamed Dadkhah-Aghdash ., Zahid Mehmood, Ayesha Iqbal, Zarbab Zulfiqar, Bin Yameen, Hafiz Naveed Anjum, Sohaib Muhammad, Muhammad Luqman, Andleeb Anwar Sardar, Muhammad Umer Farooq

Journal of Bioresource Management

Pesticides are specially designed to control pests and help in increasing the yield of crops. This study aims to investigate the genotoxic effect of the major ingredients of commonly used pesticides (Profenophos and Cypermethrin) on Allium cepa L. in different regions of the province of Punjab, Pakistan. Profenophos and Cypermethrin pesticides are extensively used by local farmers. six different dilutions of Profenophos and Cypermethrin pesticide ranging from, 0.5 ml/L, 1 ml/L, 1.5 ml/L, 2 ml/L, 2.5 ml/L and 3.0 ml/L were applied initially on seeds germination. Seeds grown in a 3.0 ml/L concentration showed a lower radical length percentage as …

Analysis Of Micro(Nano)Plastics In Food Using Raman Microscopy, Xin Guo

Doctoral Dissertations

Microplastic pollution has emerged as a global concern in both aquatic and terrestrial environments. The presence of microplastics in the food chain poses a potential threat to human health. To accurately assess the risks associated with microplastics, it is crucial to have a reliable analytical technique capable of detecting, quantifying, and identifying microplastics of varying materials, sizes, and shapes in environmental, agricultural, and food samples. Spectroscopic techniques, specifically vibrational spectroscopy (Raman and Infrared), are extensively utilized in microplastic analysis. These techniques enable rapid and high-throughput identification, while also providing size and shape information. However, there are certain gaps in the …

Usage Of Almond And Thyme Oils For The Enhancement Of Shelf Life Of Different Fish Species, Aneeza Kanwal, Roheela Yasmeen, Saima Nazir, Shumaila Usman, Bushra Nisar Khan

Journal of Bioresource Management

Fish is an important source of protein for millions of people around the globe and it's often a healthier option compared to some other meats. However, the fish industry is facing a storage challenge in the form of rapid spoilage and deterioration of freshness due to bacterial growth and enzymatic activity. The usage of oils in fish can enhance shelf life by creating a protective barrier. This research was designed to see the effects of thyme and almond oil on nutritional profile and shelf life of fish. The three fish species rohu (Labeo rohita), Mali (Wallago attu) …

The Impact Of A Non-Ionic Adjuvant To The Persistence Of Pesticides On Produce Surfaces, Daniel Barnes

Masters Theses

Adjuvants can enhance the performance of the pesticide active ingredients in many ways including decreasing surface tension and reducing evaporation. Understanding how adjuvants effect pesticide behavior (e.g., surface persistence) is crucial for developing effective pesticide formulations, as well as facilitating the development of effective approaches to reduce pesticide residues from the surface of fresh produce post-harvest. The objective of this study is to investigate the effect of a non-ionic surfactant, Surf-Ac 910, on the persistence of two model pesticides, thiabendazole and phosmet on apple surfaces. The result shows that the addition of Surf-Ac 910 increased both the maximum wetted area …

Biological Control Of Three Major Cucumber And Pepper Pests: Whiteflies, Thrips, And Spider Mites, In High Plastic Tunnels Using Two Local Phytoseiid Mites, Yusuf Abou Jawdah, Nour Ezzedine, Aya Fardoun, Samer Kharroubi, Hana Sobh, Hagop S. Atamian, Margaret S. Skinner, Bruce Parker

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

To enhance food security, food safety, and environmental health, a bio-based integrated pest management (BIPM) strategy was evaluated at two coastal locations in Lebanon as an alternative to toxic pesticide sprays in commercial high-arched plastic tunnels common in many countries. The evaluation occurred during two cucumber and pepper cropping seasons: spring and fall. At each site, two commercial tunnels were used; farmers’ conventional practices were applied in one tunnel, while the BIPM approach was followed in the second tunnel. In the farmers’ practices, a total of 14 sprays of insecticide/acaricide mixtures were applied during the spring growing season, and 6 …

An Acute Herb-Drug Interaction Of Magnoliae Officinalis Cortex With Methotrexate Via Inhibiting Multidrug Resistance-Associated Protein 2, Chung-Ping Yu, Pei-Ying Li, Szu-Yu Chen, Shiuan-Pey Lin, Ying-Chen Chen, Lu-Ching Ho, Yow-Wen Hsieh, Yu-Chi Hou

Journal of Food and Drug Analysis

Magnoliae Officinalis Cortex (MOC), an herbal drug, contains polyphenolic lignans mainly magnolol (MN) and honokiol (HK). Methotrexate (MTX), a critical drug for cancers and autoimmune deseases, is a substrate of multidrug resistance-associated protein 2 (MRP2) and breast cancer resistance protein (BCRP). This study investigated the effect of coadministration of MOC on the pharmacokinetics of MTX and relevant mechanisms. Sprague-Dawley rats were orally administered MTX alone and with single dose (2.0 and 4.0 g/kg) and repeated seven doses of MOC (2.0 g/kg thrice daily for 2 days, the 7th dose given at 0.5 h before MTX). The serum concentrations of MTX …

An Auxiliary Strategy Of Partial Least Squares Regression In Pharmacokinetic/Pharmacodynamic Studies: A Case Of Application Of Guhong Injection In Myocardial Ischemia/Reperfusion Rats, Hai-Yang Chen, Chang Li, Chong-Yu Shao, Yu-Jia Wu, Hai-Tong Wan, Yu He

Journal of Food and Drug Analysis

Guhong injection (GHI) has been applied in the therapy of cardio-cerebrovascular disease in clinic, but there is no report about the pharmacokinetic/pharmacodynamic (PK/PD) research on GHI treating myocardial ischemia/reperfusion (MI/R) injury in rats. In this study, eight compounds of GHI in plasma, including N-acetyl-L-glutamine (NAG), chlorogenic acid (CGA), hydroxysafflor yellow A (HSYA), p-coumaric acid (pCA), rutin, hyperoside, kaempferol-3-O-rutinoside, and kaempferol-3-O-glucoside, were quantified by LC-MS/MS. We discovered that the values of t_1/2β, k₁₂, V₂, and CL₂ were larger than those of t_1/2α, k₂₁, V₁, and …

Ionizable Copolymer Functionalized Magnetic Nanocomposite As An Adsorbent For Boosting The Extraction Selectivity Of Aristolochic Acids, Qi-Yue Xie, Yang Chen, Chang-Jun Li, Jia-Bin Zhang, Xiu-Jun Cao, Jun Lu

Journal of Food and Drug Analysis

Aristolochic acid nephropathy (AAN) has drawn increasing public attention. Organic anion transporters (OATs) are considered to be responsible for mediating nephrotoxicity of aristolochic acids (AAs), as AAs are typical OAT1 substrates that exhibit anionic properties and contain one hydrophobic domain. Inspired by the OAT1 three-dimensional structure or substrate/protein interactions involved in transport, we designed a magnetic polymeric hybrid, mimicking the effect of basic and aromatic residues of OAT1, for efficient enriching aristolochic acid I (AA I) and aristolochic acid II (AA II) in Traditional Chinese patent medicines (TCPM). N, N-dimethylaminopropyl acrylamide (DMAPAm) was used as a cationic monomer and copolymerized …

Effect Of Indian Gooseberry Extract On Improving Methylglyoxal-Associated Leptin Resistance In Peripheral Tissues Of High-Fat Diet-Fed Rats, Sheng-Yi Chen, Yue-Ning Huang, Jer-An Lin, Gow-Chin Yen

Journal of Food and Drug Analysis

Increased leptin resistance and methylglyoxal (MG) levels are observed in obese patients. However, whether MG deposits contribute to leptin resistance, oxidative stress, and inflammation in peripheral tissues remains unclear. In addition, the edible fruit of Indian gooseberry (Phyllanthus emblica L.) contains abundant bioactive components such asvitamin C, β-glucogallin (β-glu), gallic acid (GA), and ellagic acid (EA). Water extract of Indian gooseberry fruit (WEIG) and GA has been shown to improve cognitive decline by suppressing brain MG-induced insulin resistance in rats administered a high-fat diet (HFD). Accordingly, this study investigated the functions of WEIG and GA in inhibiting MG-induced leptin …

Pomelo (Citrus Grandis (L.) Osbeck) Sponge Layers As A Potential Source Of Soluble Dietary Fiber: Evaluation Of Its Physicochemical, Structural And Functional Properties, Xiaowei Chen, Jiajia Chen, Jian Peng, Yuanshan Yu, Jijun Wu, Jing Wen, Zhiying Kang, Yanhui Wang, Yujuan Xu, Lu Li

Journal of Food and Drug Analysis

Pomelo sponge layer (PSL) had been considered as a potential source of soluble dietary fiber (SDF), while they were mostly disposed of as waste. To promote high-value utilization of pomelo wastes, this study extracted SDF from PSL of six varieties of pomelo, and their physicochemical, structural and functional properties were investigated. Results indicated that all PSL-SDFs showed good physicochemical and functional properties. Among them, PSL-SDF from grapefruit (GRSDF) showed better water holding capacity and swelling capacity, whereas Shatian pomelo PSL-SDF and Guanxi pomelo PSL-SDF had the highest thermal stability and oil holding capacity, respectively. Furthermore, compared with other PSL-SDFs, GRSDF …

Application And Effectiveness Of Artificial Intelligence For The Border Management Of Imported Frozen Fish In Taiwan, Wen-Chin Tu, Wan-Ling Tsai, Chi-Hao Lee, Chia-Fen Tsai, Jen-Ting Wei, King-Fu Lin, Shou-Mei Wu, Yih-Ming Weng

Journal of Food and Drug Analysis

In Taiwan, the number of applications for inspecting imported food has grown annually and noncompliant products must be accurately detected in these border sampling inspections. Previously, border management has used an automated border inspection system (import food inspection (IFI) system) to select batches via a random sampling method to manage the risk levels of various food products complying with regulatory inspection procedures. Several countries have implemented artificial intelligence (AI) technology to improve domestic governmental processes, social service, and public feedback. AI technologies are applied in border inspection by the Taiwan Food and Drug Administration (TFDA). Risk management of border inspections …

Trans-2-Nonadecyl-4-(Hydroxymethyl)-1,3-Dioxolane (Tnhd) Purified From Freshwater Clams Markedly Alleviates Dimethylnitrosamine-Induced Hepatic Fibrosis, Ya-Ru Kuo, Chen-Yu Tsai, Wei-Sheng Lin, Chi-I Chang, Ching-Shu Lai, Min-Hsiung Pan

Journal of Food and Drug Analysis

Liver fibrosis occurs due to injury or inflammation, which results in the excessive production of collagen and the formation of fibrotic scar tissue that impairs liver function. Despite the limited treatment options available, freshwater clams may hold promise in the treatment of liver fibrosis. In this study, we demonstrated the effects of ethanol extract of freshwater clam (FCE), ethyl acetate extract of FCE (EA-FCE), and trans-2-nonadecyl-4-(hydroxymethyl)-1,3-dioxolane (TNHD) on liver fibrosis induced by dimethylnitrosamine (DMN). Administration of FCE and TNHD alleviated liver injury, including tissue damage, necrosis, inflammation scores, fibrosis scores, serum enzymes, and triglyceride levels. Furthermore, we analyzed the expression …

Mushrooom Production Without Solid Substrates, Alexander Baena, Marshall Porterfield

Graduate Industrial Research Symposium

Novel biotechnological approaches are needed to enhance renewable food production and waste recycling capabilities in resource-constrained environments. Edible mushrooms possess nutritious profiles and represent emerging opportunities to develop circular bioproduction by transforming waste organic materials into food and high-value products. However, traditional mushroom cultivation methods utilizing solid substrates have technical limitations like 1) uneven nutrient diffusion, 2) constant sterilization needs, 3) numerous intermediate steps that are energy and time-demanding, and 4) waste production from bags and jars. This work introduces an innovative hydroponic fungal cultivation system called Mycoponics™ using bioengineered ceramic materials. This liquid-nutrient-based technique optimizes fungal metabolism and facilitates …

Online Class-Incremental Learning For Real-World Food Image Classification, Siddeshwar Raghavan, Jiangpeng He, Fengqing Zhu

Graduate Industrial Research Symposium

Food image classification is essential for monitoring health and tracking dietary in image-based dietary assessment methods. However, conventional systems often rely on static datasets with fixed classes and uniform distribution. In contrast, real-world food consumption patterns, shaped by cultural, economic, and personal influences, involve dynamic and evolving data. Thus, it requires the classification system to cope with continuously evolving data. Online Class Incremental Learning (OCIL) addresses the challenge of learning continuously from a single-pass data stream while adapting to the new knowledge and reducing catastrophic forgetting. Experience Replay (ER) based OCIL methods store a small portion of previous data and …

Effects Of Storage Conditions On Chemistry And Technological Properties Of Different Cultivars Of Chickpea, Shirin Kazemzadeh Pournaki, Atanu Biswas, Clifford Hall

Dairy Science and Food Science Faculty Publications: 2022 to Present

The impact of storage conditions, such as 40 °C, high humidities (55% and 65%), and storage (360 days), on the nutritional value, pasting, and functional properties, color differences, and quality of protein and starch in five Chickpea (Cicer arietinum) cultivars (Crown, Royal, Sierra, Orion, and Frontier) was determined. The Sierra cultivar had the highest initial moisture content (MC, 7.7 ± 0.01%), and MC increased over time for all samples stored at 55% and 65% RH and 21 °C. Protein (PC), total starch (TSC), and fat (FC) contents changed in all cultivars during storage. Under the same storage conditions …

Antioxidant Properties Of Kombucha Beverage Infused With Ganoderma Lucidum And Green Tea From Camellia Sinensis (L.) Kuntze With Several Fermentation Times, Rizki Rabeca Elfirta, Pamungkas Rizki Ferdian, Iwan Saskiawan, Tri Hadi Handayani, Kayla Faza Gustafri Mandalika, Rini Riffiani, Kasirah Kasirah, Ukhradiya Magharaniq Safira Purwanto

Karbala International Journal of Modern Science

Kombucha is a functional beverage produced through the fermentation of infused tea or Camelia sinensis (L.) Kuntze (CS) by a symbiotic culture of bacteria and yeast. Apparently, the substrate of kombucha can be substituted to enhance its functional properties. Ganoderma lucidum (GL) is a potential substrate reported to have health benefits. This study aims to evaluate antioxidant properties by comparing different formulations and fermentation times. The formulations prepared in the present study varied in the compositions of GL and CS substrates, namely F1 (100% GL), F2 (75% GL: 25% CS), F3 (50% GL: 50% CS), F4 (25% GL: 75% CS), …