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Full-Text Articles in Food Science

Analysis Of Micro(Nano)Plastics In Food Using Raman Microscopy, Xin Guo Mar 2024

Analysis Of Micro(Nano)Plastics In Food Using Raman Microscopy, Xin Guo

Doctoral Dissertations

Microplastic pollution has emerged as a global concern in both aquatic and terrestrial environments. The presence of microplastics in the food chain poses a potential threat to human health. To accurately assess the risks associated with microplastics, it is crucial to have a reliable analytical technique capable of detecting, quantifying, and identifying microplastics of varying materials, sizes, and shapes in environmental, agricultural, and food samples. Spectroscopic techniques, specifically vibrational spectroscopy (Raman and Infrared), are extensively utilized in microplastic analysis. These techniques enable rapid and high-throughput identification, while also providing size and shape information. However, there are certain gaps in the …


The Impact Of A Non-Ionic Adjuvant To The Persistence Of Pesticides On Produce Surfaces, Daniel Barnes Mar 2024

The Impact Of A Non-Ionic Adjuvant To The Persistence Of Pesticides On Produce Surfaces, Daniel Barnes

Masters Theses

Adjuvants can enhance the performance of the pesticide active ingredients in many ways including decreasing surface tension and reducing evaporation. Understanding how adjuvants effect pesticide behavior (e.g., surface persistence) is crucial for developing effective pesticide formulations, as well as facilitating the development of effective approaches to reduce pesticide residues from the surface of fresh produce post-harvest. The objective of this study is to investigate the effect of a non-ionic surfactant, Surf-Ac 910, on the persistence of two model pesticides, thiabendazole and phosmet on apple surfaces. The result shows that the addition of Surf-Ac 910 increased both the maximum wetted area …


Detection And Control Of Environmentally Transmissible Viruses, Anand R. Soorneedi Nov 2023

Detection And Control Of Environmentally Transmissible Viruses, Anand R. Soorneedi

Doctoral Dissertations

Viruses, owing to their ubiquitous nature and ability to infect almost every other species, have long been a subject of interest for scientists. Some of the virus species can be very deadly to humans and animals alike and can impose a huge economic and health burden across the world. The recent CoVID-19 pandemic underscores the importance of timely detection for developing effective intervention strategies. Unfortunately, some of the virus species that cause significant health and economic impacts do not have robust and reliable detection methods due to several reasons. In some cases, despite having gold standard methods for detection of …


Understanding And Developing Safer Sanitation Agents And Strategies In Food Production Environments, Pragathi Kamarasu Nov 2023

Understanding And Developing Safer Sanitation Agents And Strategies In Food Production Environments, Pragathi Kamarasu

Doctoral Dissertations

Food is a primary resource for survival of human beings, and it is also one of the primary resources for spread of infectious diseases. When both these factors come together it does not only cause ill effects on our health but also causes a burden on the food and agricultural industry. Through the help of science, us researchers and scientists have always tried to reduce this burden. And I hope like a drop of water in the ocean in some way my research contributes towards this. According to WHO currently in 2023 there are about 1 in 10 people falling …


Analysis Of Antioxidant Synergism And Its Mechanisms In Different Food Systems, Ipek Bayram Nov 2023

Analysis Of Antioxidant Synergism And Its Mechanisms In Different Food Systems, Ipek Bayram

Doctoral Dissertations

Lipid oxidation results in off-flavors, toxic aldehydes, and co-oxidation of proteins and color compounds. Combining antioxidants to achieve synergistic interactions has been practiced for decades to improve oxidative stability. Nevertheless, synergism mechanisms have been poorly understood and rarely studied. This dissertation examines the mechanisms of antioxidant synergism in a model system with α-tocopherol (α-TOC) and myricetin (MYR). The interactions between α-tocopherol and taxifolin (TAX) were also tested because it has structural similarities to myricetin but has a higher redox potential. The first part of this research focused on the antioxidant interactions between α-tocopherol and myricetin in stripped soybean oil-in-water emulsions …


Evaluating The Potential Of Ozone Microbubbles For Inactivation Of Tulane Virus, A Human Norovirus Surrogate, Bozhong Guan Nov 2023

Evaluating The Potential Of Ozone Microbubbles For Inactivation Of Tulane Virus, A Human Norovirus Surrogate, Bozhong Guan

Masters Theses

Microbubbles are small gas-filled bubbles with diameters ranging from 50 to 1 μm, and less than 200 nm are called nanobubbles. Their small sizes and large specific surface area result in a high gas dissolution rate and long lifetime in liquid. Ozone is a strong oxidant that destroys microorganisms and only produces oxygen as the final by-product in fresh water. However, due to the poor stability of aqueous ozone, critical gas waste happens during treatments which leads to a high economic loss. Microbubbles have shown promising enhancement of ozone treatment. In previous studies, ozone microbubbles exhibited excellent efficacy in the …


Impact Of Fermented And Non-Fermented Plant-Based Foods Supplementation On Gut Microbiota And Metabolites In C57bl/6j Mice, Priya Darshan Gandhi Nov 2023

Impact Of Fermented And Non-Fermented Plant-Based Foods Supplementation On Gut Microbiota And Metabolites In C57bl/6j Mice, Priya Darshan Gandhi

Masters Theses

Plant-based proteins have gained popularity because of their high nutritional value and more sustainable alternative to animal-based proteins. Soybean and chickpea are two widely consumed plant-based proteins, whereas tempeh is a popular plant-based fermented whole food product that is rich in protein. With the increase in the development of plant-based food products, there is little research into how plant proteins affect gut microbiota characteristics and metabolites. Therefore, there is a need to understand the underlying mechanisms surrounding the consumption of these foods. The purpose of this study was to investigate the health benefits of soybean, chickpea, and their tempeh counterparts’ …


Sucrose Concentration And Fermentation Temperature Impact The Sensory Characteristics And Liking Of Kombucha, Gil Cohen Nov 2023

Sucrose Concentration And Fermentation Temperature Impact The Sensory Characteristics And Liking Of Kombucha, Gil Cohen

Masters Theses

Kombucha is a fermented tea beverage consumed for its probiotics and functional properties and has a unique sensory profile driven by the properties of tea polyphenols and fermentation products, including organic acids. Fermentation temperature and sucrose content affect the fermentation process and the production of organic acids, yet less is known about the impact on the sensory profile and consumer acceptance. Thus, we aim to examine the impact of sucrose concentration and fermentation temperature on sensory attributes and liking. For this study, kombucha tea was fermented at three different concentrations of sucrose and fermented at two temperatures for 11 days. …


Sucrose Concentration And Fermentation Temperature Impact The Sensory Characteristics And Liking Of Kombucha - Data, Gil Cohen, David A. Sela, Alissa A. Nolden Aug 2023

Sucrose Concentration And Fermentation Temperature Impact The Sensory Characteristics And Liking Of Kombucha - Data, Gil Cohen, David A. Sela, Alissa A. Nolden

Data and Datasets

In this data set we can see the pH, brix, and titratable acidity measurements in triplicate, as well as the sensory study data. The kombucha batches were combined prior to sensory testing and measured for their combined titratable acidity as well.


Impact Of Animal Protein And Plant Protein On The Gut Microbiota And Metabolites Of C57bl/6j Mice, Levina Soetyono Aug 2023

Impact Of Animal Protein And Plant Protein On The Gut Microbiota And Metabolites Of C57bl/6j Mice, Levina Soetyono

Masters Theses

Plant-derived protein has gained popularity in recent years due to its health and environmental impact. Studies comparing the health benefits of animal and plant protein have mostly focused on soy as a plant protein representative due to its popularity. Demand for other protein sources such as peas and fava beans has been increasing; thus, the health impact of plant protein sources other than soy must be assessed. Evidence has shown that diet influences the gut microbiota and the metabolites in the body. Metabolites associated with amino acids are strong contributors to the metabolite distinction between dietary animal and plant foods. …


Bifidobacterial Metabolism Of Fucosylated Human Milk Oligosaccharides Influences Structure And Function Of The Infant Gut Microbiome, Liv R. Dedon Apr 2023

Bifidobacterial Metabolism Of Fucosylated Human Milk Oligosaccharides Influences Structure And Function Of The Infant Gut Microbiome, Liv R. Dedon

Doctoral Dissertations

Human milk contains human milk oligosaccharides (HMOs) that are indigestible and pass intact through the infant gastrointestinal tract where they are available for microbial metabolism. HMOs incorporate the same monosaccharide building blocks but vary structurally in primary sequence of monomeric components. Primary sequences are further diversified by degree of polymerization, branching, and secondary modifications such as fucosylation. Fucosylated HMOs (fHMOs) are highly abundant and can account for over 30% of total HMOs. Infant-colonizing Bifidobacterium longum subsp. infantis (B. infantis) possesses a specialized gene cluster conveying the ability to metabolize fHMOs. This study presents an in-depth analysis of B. …


The Genomic Dynamics Of Fermented Food Microbes, Katherine Chacón-Vargas Apr 2023

The Genomic Dynamics Of Fermented Food Microbes, Katherine Chacón-Vargas

Doctoral Dissertations

Along with the agriculture and domestication revolution, humans have utilized bacteria, yeasts, and molds for millennia in the production of traditionally fermented foods and beverages. Fermentation is a very ancient practice of high relevance nowadays since it contributes with a great variety of foods worldwide. Microbial fermentation allows metabolic transformation of the raw food materials leading to biochemical changes that played a key role in food preservation, health benefits, nutrition, flavors, and texture, among others. Food fermentation practices could diverge from traditional artisanal spontaneous fermentation to industrialize methods with specialized microbial starters and although fermented environments tend to be very …


Analysis Of Food Colorants Using Raman Spectroscopy, Haochen Dai Oct 2022

Analysis Of Food Colorants Using Raman Spectroscopy, Haochen Dai

Doctoral Dissertations

Color is one of the most important quality attributes that affect consumers' selection of food. The increasing demand of consumers for natural colorants over artificial ones has placed challenges in both product development and regulatory practices. However, current analytical solutions for food colorants are mostly limited to a sophisticated laboratory setting with tedious sample preparation procedures. Herein, this research focuses on the analytical developments toward cost-effective determination of colorant adulteration and stability analysis. The main technique explored is Raman spectroscopy, which measures the inelastic light scattering and allows one to obtain unique molecular fingerprints for specific molecules. Compared with chromatographic …


Novel Approaches For Preventing Lipid Oxidation In Emulsion-Based Food Systems, Mitchell D. Culler Oct 2022

Novel Approaches For Preventing Lipid Oxidation In Emulsion-Based Food Systems, Mitchell D. Culler

Doctoral Dissertations

Consumer interest in “clean” labels has continued to be a key driver of consumer behavior and purchasing habits over the past decade. Food manufacturers are therefore eager to replace synthetic antioxidants such as ethylenediaminetetraacetic acid (EDTA) with natural alternatives, however alternative chelators that will bind iron at low pH remain elusive. Coupled with the current oil shortages and supply chain challenges that have arisen recently, there is an urgent need for innovative solutions to increase the oxidative stability of edible oils. One available strategy is diluting oils high in unsaturated fatty acids into more stable, more saturated oils, thus delaying …


Foaming Properties Of Dilute Pea Protein Solutions, Jiani Bao Jun 2022

Foaming Properties Of Dilute Pea Protein Solutions, Jiani Bao

Masters Theses

Plant-derived protein such as pea protein is a promising replacement for animal protein and is becoming popular in recent years because of its high nutritional value and potential reduction of environmental footprint. However, the increasing demand for plant-derived proteins is accompanied by the increase of wastes during protein processing such as wastewater containing dilute protein content, which may raise the cost for the downstream processing. Therefore, there is an emerging need to develop novel processing strategies to reduce waste while valorizing useful ingredients. Several researchers suggest that foam fractionation technology can be a viable approach to extract and concentrate protein …


Enhancement In Photoinactivation Of Food-Borne Pathogenic Bacteria With The Use Of Curcumin And Surfactants, Victor Ryu Jun 2022

Enhancement In Photoinactivation Of Food-Borne Pathogenic Bacteria With The Use Of Curcumin And Surfactants, Victor Ryu

Doctoral Dissertations

Photosensitizers and UV or visible light could photoinactivate bacteria. Curcumin was utilized as a food-grade photosensitizer, and an enhancement of its water-dispersibility, chemical stability, and antimicrobial activity when introduced into surfactant solutions was observed. Stock curcumin-surfactant solutions were prepared by titrating curcumin dissolved in ethanol into either Surfynol 465 or Tween 80 solutions (5 mM sodium citrate buffer). Stock curcumin surfactant solutions with different surfactant concentrations were diluted to 1 µM prior to irradiation. The resulting solutions also had surfactant concentrations below, near, and above their critical micelle concentrations (CMCs) of the respective surfactants. The antimicrobial activity of the curcumin-loaded …


Detection Of Benzoyl Peroxide In Flour Using Raman Spectroscopy, Yu Ho Mar 2022

Detection Of Benzoyl Peroxide In Flour Using Raman Spectroscopy, Yu Ho

Masters Theses

Benzoyl peroxide (BPO) is a common bleaching agent used in wheat flour. Due to its ability to damage existing nutrients in food and potential adverse effect to health, BPO have been strictly banned as a food additive in several countries and regions, such as China and Europe. However, the United States specifies that BPO is generally recognized as safe (GRAS). So, the WHO/FAO created a Codex Alimentarius Commission (CAC) to regulate the international BPO usage standard. According to the CAC, it is restricted at 75 mg/kg or parts per million (ppm). BPO is very unstable and easily converts to benzoic …


Double Emulsions Relevant To Food Systems: Fabrication, Characterization, And Applications, Jinning Liu Mar 2022

Double Emulsions Relevant To Food Systems: Fabrication, Characterization, And Applications, Jinning Liu

Doctoral Dissertations

Systems of the W/O/W type continue to be of growing interest to food scientists and technologists for two main reasons. First, there is the possibility of preparing a reduced-fat emulsion product by the replacement of a conventional oil-in-water emulsion by the equivalent W/O/W emulsion having a lower actual oil content but a similar in-mouth perceived texture. Second, there is the capability to encapsulate and protect a sensitive hydrophilic bioactive compound. However, it is always a bottleneck to obtain robust formulations using economically viable ingredients and processing operations. To improve its performance for practical applications in food production, efforts have been …


Detection Of Mycotoxins Using Surface-Enhanced Raman Spectroscopy, Lourdes B. Martinez Rojas Mar 2022

Detection Of Mycotoxins Using Surface-Enhanced Raman Spectroscopy, Lourdes B. Martinez Rojas

Doctoral Dissertations

Mycotoxins are toxic metabolites produced by fungus that can be parasites or saprophytes of crops or livestock forage. Consumer demand for plant-based foods and interest in animal-based foods originating from animals fed plant-based feed has been on the rise. Therefore, monitoring mycotoxins occurring in the food supply is more critical than ever. The goal of this project is to improve surface-enhanced Raman spectroscopy’s (SERS) ability to identify and detect mycotoxins using label-free SERS substrates. Two simple approaches were designed to enhance the detection of mycotoxins produced by the Aspergillus and Penicillium genera, ochratoxin A and aflatoxin B1. Ochratoxin A was …


Analysis Of Plant And Animal Proteins Using Raman Spectroscopy, Noopur Bapardekar Mar 2022

Analysis Of Plant And Animal Proteins Using Raman Spectroscopy, Noopur Bapardekar

Masters Theses

There has been a notable rise in the alternative protein market in the recent years which promotes an interest in the research of both animal and plant proteins to establish better structure-function relationships. Over the years many analytical tools have been used to study proteins and compare them, however Raman Spectroscopy and Surface Enhanced Raman Spectroscopy (SERS) have not been as much used for this application. SERS consolidates Raman Spectroscopy that primarily measures molecular vibrations and nanostructures that enhance the weak Raman signals. The objective of this study is to explore the capability of the Raman instrumentation in combination with …


Fate And Effect Of Food-Grade Titanium Dioxide Particles/Polystyrene Nanoplastics On Nutrients Bioaccessibility Of Food In The Simulated Human Gastrointestinal Tract, Chunyang Li Feb 2022

Fate And Effect Of Food-Grade Titanium Dioxide Particles/Polystyrene Nanoplastics On Nutrients Bioaccessibility Of Food In The Simulated Human Gastrointestinal Tract, Chunyang Li

Doctoral Dissertations

Food grade titanium dioxide particles (E171), as whitening agent, are commonly used in chewing gums, candies, sauces, salad dressings, and powdered milk. Recently, nanoplastics (NPs) (defined as < 1 µm), which are degraded from plastic debris undergoing environmental process, have received global attention. Because nanoplastics are ubiquitous in aquatic and terrestrial systems, and have been detected in marine animals, table salts, drinking water, and air. Thus, the presence of E171 as additives in the food, and the nanoplastics in food chains, both pose potential risks to human health through retarded processes in the gastrointestinal tract (GIT). However, the knowledge about the fate of E171 or nanoplastics and their impact on digestive enzymes activity or nutrient bioaccessiblity are currently limited. Therefore, it is necessary to investigate potential impacts of E171 or nanoplastics on digestion processes and nutrient bioaccessibility in the simulated human gastrointestinal tract, which includes mouth, stomach and intestinal phases, to mimic the digestion processes of food incubating with E171 or polystyrene (PS) nanoplastics. The objectives of my dissertation and major findings are presented below: 1) The impacts of E171 on lipid digestion and vitamin D3 (VD3) bioaccessibility encapsulated within oil-in-water emulsions in a simulated human gastrointestinal tract model were explored; 2) The impacts of E171 on the bioaccessibility of minerals (Ca, K, Mg, Fe, Mn, Zn, P and S) released from spinach leaves using a simulated human digestion tract were investigated; 3) The impacts of various concentrations or different functional group (-COOH, -NH2) of PS NPs on the starch hydrolysis in the simulated …


Mapping Selected Polyphenols Metabolism By Gut Bacteria And Their Genes, Ermin Zhao Feb 2022

Mapping Selected Polyphenols Metabolism By Gut Bacteria And Their Genes, Ermin Zhao

Doctoral Dissertations

The human gut microbiome is a huge enzyme repository for dietary polyphenols metabolism, especially considering most of the polyphenols cannot be digested in the host and their biological functions are limited. Poor bioaccessibility based on traditional pharmaceutical ADME (absorption, distribution, metabolism, and excretion) assessment is the main problem facing the widely medical application of most polyphenols. Gut bacteria have the potential to mediate a wide range of biotransformation reactions of polyphenols, which leads to the production of many bioactive metabolites. In the past decades, mounting evidence in traditional ADME study have demonstrated gut bacteria play an irreplaceable role in dietary …


Antibiotic Sensitivity Testing Of Foodborne Bacteria Using Surface-Enhanced Raman Spectroscopy, Joshua Gukowsky Oct 2021

Antibiotic Sensitivity Testing Of Foodborne Bacteria Using Surface-Enhanced Raman Spectroscopy, Joshua Gukowsky

Doctoral Dissertations

The spread of antibiotic resistant bacteria around the world has become a major public health issue, and it is essential that effective detection methods exist for identifying these organisms and preventing them from spreading throughout our food systems and into the environment. The goal of this research is to develop a novel analytical procedure that is capable of easily identifying antibiotic resistance in bacterial samples, and also provides more information about the biochemical characteristics of the bacteria and their responses to antibiotic exposure. Surface-enhanced Raman Spectroscopy (SERS), an analytical technique that uses light scattering to produce a spectrum based on …


Factors Affecting Sensory Acceptance Of Thickened Liquids Used In Dysphagia Management, Allison N. Cox Oct 2021

Factors Affecting Sensory Acceptance Of Thickened Liquids Used In Dysphagia Management, Allison N. Cox

Masters Theses

Between 4-16% of adults in the United States have experienced difficulty swallowing at some point during their lives. Difficulty swallowing, or clinically referred to as dysphagia, poses increased concern when drinking beverages. While no treatment is currently available, it is often recommended that liquids be thickened to improve the safety of swallowing and prevent liquids from being aspirated in the lungs. However, thickened liquids are poorly accepted by individuals with dysphagia. Taste and flavor suppression has been shown in various thickened liquid matrices, but the mechanisms for understanding these changes in perception are quite complex. Additionally, literature focused on dysphagic …


Antioxidant Synergism Between Α-Tocopherol And A High Phosphatidylserine Modified Lecithin, Harshika Arora Oct 2021

Antioxidant Synergism Between Α-Tocopherol And A High Phosphatidylserine Modified Lecithin, Harshika Arora

Masters Theses

Phospholipids, such as phosphatidylserine (PS) have been shown to work synergistically with tocopherols to extend the shelf life of oil-in-water emulsions. However, the high cost of PS prevents it from being used as a food additive. This work investigated the potential use of a high PS enzyme-modified lecithin to be used along with α-tocopherol to extend the lag phase of oil-in-water emulsions stabilized using Tween 20. Phospholipase D from Streptomyces sp. and L-serine were used to modify lecithin to increase PS concentration. Enzyme activity was optimized as a function of pH and temperature using a high PC soybean lecithin. The …


Antioxidant Combination Of High Phosphatidylserine (Ps) Lecithin With Mixed Tocopherol In Soybean Oil-In-Water Emulsion: Effect Of Ph And Salt, Princy Agnihotri Oct 2021

Antioxidant Combination Of High Phosphatidylserine (Ps) Lecithin With Mixed Tocopherol In Soybean Oil-In-Water Emulsion: Effect Of Ph And Salt, Princy Agnihotri

Masters Theses

Lipid oxidation is one of the major challenges faced by the food industry as it contributes to the loss of nutritional quality and loss of flavor in food products. Studies have shown that naturally occurring phospholipids like phosphatidylserine (PS) and phosphatidylethanolamine (PE) can regenerate oxidized tocopherols and help delay the lipid oxidation in bulk oils and oil-in-water emulsions. Since consumers desire simpler and cleaner labels, without chemically synthesized antioxidants, this research is of great interest. The combination of PS and PE with tocopherols has already been studied. However, PS was a better antioxidant in combination with tocopherols in the oil-in-water …


Fat Lowering Effects Of Fisetin In Caenorhabditis Elegans, Nikolas J. Rodriguez Jul 2021

Fat Lowering Effects Of Fisetin In Caenorhabditis Elegans, Nikolas J. Rodriguez

Masters Theses

Fisetin, a flavanol with anti-inflammatory, anti-cancer, and anti-aging properties, has shown promise for reducing fat accumulation in tissue culture and animal models. This plant sourced compound has limited studies supporting its effects on fat accumulation. Therefore, this study was completed to determine fisetin’s role in fat reduction along with its mechanism of action using Caenorhabditis elegans. C. elegans is a small roundworm with roughly 65% of its genes being conserved in humans related to disease. In this study, 100 and 200 µM fisetin has shown to reduce fat accumulation in wild-type worms. Body size, locomotion, and pumping rate were …


Improvement Of Solubility, Stability, And Bioaccessibility Of Curcumin Using Colloidal Delivery Systems, Bingjing Zheng Jul 2021

Improvement Of Solubility, Stability, And Bioaccessibility Of Curcumin Using Colloidal Delivery Systems, Bingjing Zheng

Doctoral Dissertations

Curcumin is a yellow-orange crystalline substance found in certain foods (turmeric) that is claimed to exhibit a broad range of biological activities. Its application as a nutraceutical in functional foods and beverages is often limited by its relatively low solubility in aqueous media, its chemical instability, and its low bioavailability. Recent research suggests that colloidal delivery systems can overcome these hurdles and improve the efficacy and commercial value of curcumin in the food, supplement, and pharmaceutical fields. The purpose of this research was to develop colloidal delivery systems to improve the application of curcumin as a nutraceutical in foods. First, …


Investigation On The Factors Impacting The Bioaccessibility Of Oil-Soluble Vitamins: Food Matrix Effects, Yunbing Tan Jul 2021

Investigation On The Factors Impacting The Bioaccessibility Of Oil-Soluble Vitamins: Food Matrix Effects, Yunbing Tan

Doctoral Dissertations

Food, nutrition, and pharmaceutical scientists are trying to elucidate the major factors impacting the bioavailability of macronutrients (e.g., lipids), micronutrients (e.g., vitamins), and nutraceuticals (e.g., carotenoids) so as to improve their efficacy. Currently, however, there is a limited understanding of how food matrix effects impact nutrient digestion and bioavailability. In this thesis, the impact of food matrix effects was elucidated by systematically examining the influence of several important factors (including oil type, oil concentration, droplet size, emulsifier type, and nutrient type) on lipid digestion and the bioaccessibility of hydrophobic bioactive agents. Oil-in-water emulsions were used as model …


Formation, Characterization, And Utilization Of Multilayer Nanoemulsions In Foods, Jorge L. Muriel Mundo Jun 2021

Formation, Characterization, And Utilization Of Multilayer Nanoemulsions In Foods, Jorge L. Muriel Mundo

Doctoral Dissertations

Multilayer coatings have been proposed as a promising nanotechnology for improving the performance of emulsion-based products in numerous research fields. In foods, these multilayer coatings can be used to improve the encapsulation and protection of bioactive ingredients in delivery systems during storage and passage through the gastrointestinal tract (1, 2). Therefore, there is strong interest in understanding the formation, properties, and performance of these novel coatings. Multilayer coatings are formed by layer-by-layer electrostatic deposition of oppositely charged biopolymers, such as proteins and polysaccharides. A better understanding of the formation and properties of biopolymer multilayer coatings could lead to …