Meat Science Commons^™

Comparison Of Wet And Dry Distillers Grains Plus Solubles To Corn As An Energy Source In Forage-Based Diets, Nerissa Ann Ahern, Brandon L. Wingeyer, Terry J. Klopfenstein, James C. Macdonald, Galen E. Erickson, Andrea K. Watson

Department of Animal Science: Faculty Publications

Four experiments compared wet or dry distillers grains plus solubles (WDGS or DDGS) to corn as energy sources in forage-based diets. In Exp. 1, 66 individually fed steers (268 kg of initial BW) were fed a 60:40 blend of sorghum silage and alfalfa hay and supplemented at 0, 0.33, 0.67, or 1.0% of BW with either WDGS or DDGS. In Exp. 2, 160 steers (286 kg of initial BW) were fed 25% WDGS or 33.6% dry rolled corn (DRC) in 35% sorghum silage and grass hay diets (DM basis). In Exp. 3, 60 individually fed steers (231 kg of initial …

Vegfa Splicing: Divergent Isoforms Regulate Spermatogonial Stem Cell Maintenance, Kevin M. Sargent, Debra T. Rozell, Ningxia Lu, William E. Pohlmeier, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Despite being well-known for regulating angiogenesis in both normal and tumorigenic environments, vascular endothelial growth factor A (VEGFA) has been recently implicated in male fertility, namely in the maintenance of spermatogonial stem cells (SSC). The VEGFA gene can be spliced into multiple distinct isoforms that are either angiogenic or antiangiogenic in nature. Although studies have demonstrated the alternative splicing of VEGFA, including the divergent roles of the two isoform family types, many investigations do not differentiate between them. Data concerning VEGFA in the mammalian testis are limited, but the various angiogenic isoforms appear to promote seminiferous cord formation and to …

Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Catherine Purcell, Timothy P. L. Smith, Tad Sonstegard, Jerry Taylor, Bhanu Telugu, Alison Van Eenennaam, Curtis P. Van Tassell, Kevin Wells

Department of Animal Science: Faculty Publications

In 2008, a consortium led by the Agricultural Research Service (ARS) and the National Institute for Food and Agriculture (NIFA) published the “Blueprint for USDA Efforts in Agricultural Animal Genomics 2008–2017,” which served as a guiding document for research and funding in animal genomics. In the decade that followed, many of the goals set forth in the blueprint were accomplished. However, several other goals require further research. In addition, new topics not covered in the original blueprint, which are the result of emerging technologies, require exploration. To develop a new, updated blueprint, ARS and NIFA, along with scientists in the …

The Role Of Direct-Fed Microbials In Conventional Livestock Production, Joe O. Buntyn, Ty Schmidt, David J. Nisbet, Todd R. Callaway

Department of Animal Science: Faculty Publications

Supplementation of direct-fed microbials (DFM) as a means to improve the health and performance of livestock has generated significant interest over the past 15+ years. A driving force for this increased interest in DFM is to reduce or eliminate the use of low-dose antibiotics in livestock production. This increased attention toward DFM supplementation has generated an extensive body of research. This effort has resulted in conflicting reports. Although there has been considerable variation in the design of these studies, one of the main causes for this lack of consistency may be attributed to the variation in the experimental immune challenge …

Determination Of Yolk Contamination In Liquid Egg White Using Raman Spectroscopy, K. Cluff, G. Konda Naganathan, D. Jonnalagada, I. Mortensen, R. Wehling, Jeyamkondan Subbiah

Department of Animal Science: Faculty Publications

Purified egg white is an important ingredient in a number of baked and confectionary foods because of its foaming properties. However, yolk contamination in amounts as low as 0.01% can impede the foaming ability of egg white. In this study, we used Raman spectroscopy to evaluate the hypothesis that yolk contamination in egg white could be detected based on its molecular optical properties. Yolk contaminated egg white samples (n = 115) with contamination levels ranging from 0% to 0.25% (on weight basis) were prepared. The samples were excited with a 785 nm laser and Raman spectra from 250 to 3,200 …

The Effect Of Copper And Zinc Source On Pre-Weaning Performance Of Cows, Health And Performance Of Suckling Calves, And Post-Weaning Feedlot Performance, Carcass Composition, And Meat Quality Of Calves, Jesse Fulton

Electronic Theses and Dissertations

During gestation an elevated level of nutrient intake is required by the cow in order to meet the requirements of the maturing fetus and if these requirements are not met, changes in fetal development can occur. However, little is understood about the effects of gestational manipulation of specific nutrients, such as minerals, on progeny growth, performance and carcass traits. Previous research supports the hypothesis that minerals provided in an organic form should be more soluble and readily available to the body for absorption. Therefore the objectives of this study were to determine if source of gestational and post-natal Cu and …

Metabolic And Body Temperature Responses To Environmental Conditions Across Seasons In Finishing Steers, Bradley M. Boyd, Terry L. Mader, Curtis J. Bittner Bittner, Dirk Burken, Henry Hilscher, Gene Wijffels, John B. Gaughan, Megan Sullivan, Judy Cawdell- Smith, Galen E. Erickson

Nebraska Beef Cattle Reports

Two trials (summer and winter) were conducted with 80 steers each at the UNL ARDC near Mead, Neb. Continuous body temperature was collected throughout both trials and blood samples were taken every other week until four weeks prior to harvest. Th is resulted in a total of 6 collections for the summer and 8 collections for the winter. Blood measures responded differently between seasons with significant quadratic interactions between collection time and season suggesting environmental conditions affect blood metabolites.

Effects Of Shade And Feeding Zilpaterol Hydrochloride To Finishing Steers On Performance, Carcass Quality, Heat Stress, Mobility, And Body Temperature, Bradley M. Boyd, Steven D. Shackelford, Kristin E. Hales Hales, Tami M. Brown- Brandl, Meredith L. Bremer, Matthew L. Spangler, Tommy L. Wheeler, David A. King, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study evaluated the effects of shade and feeding zilpaterol hydrochloride (ZH) on performance, carcass quality, mobility, and body temperature. No effect on body temperature, or performance was observed for shaded cattle versus cattle in open pens. Feeding ZH increased HCW, LM area and decreased yield grade. Zilpaterol hydrochloride increased respiration rate but did not significantly affect body temperature or mobility. Across all treatments mobility decreased with time, therefore, cattle were least mobile at the time of harvest.

Feeding Vitamin E May Reverse Sarcoplasmic Reticulum Membrane Instability Caused By Feeding Wet Distillers Grains Plus Solubles To Cattle, Michael D. Chao, Katherine I. Domenech, Chris R. Calkins Calkins

Nebraska Beef Cattle Reports

Steers were finished on either 0% or 30% wet distillers grains plus solubles with four antioxidant treatments (control; vitamin E; Agrado; vitamin E + Agrado). Feeding wet distillers grains plus solubles increased polyunsaturated fatty acids contents in SR membrane and altered sarcoplasmic reticulum phospholipid profiles. Steaks from steers fed wet distillers grains plus solubles also had more protein degradation. Supplementing vitamin E reversed the alteration of sarcoplasmic reticulum phospholipid profiles and prevented the accelerated protein degradation resulted from feeding wet distillers grains plus solubles. These findings suggest that feeding wet distillers grains plus solubles in the finishing diet may accelerate …

Granulosa Cell Exposure To Excess Androgens Inhibits Their Ability To Proliferate In The Cow Which May Cause Or Perpetuate Androgen Excess, Andrea S. Cupp, Sarah Romereim, Adam F. Summers Summers, William E. Pohlmeier, Renee M. Mcfee, Renata Spuri- Gomes, Scott G. Kurz, Anthony K. Mcneel, Robert A. Cushman, John S. Davis, Jennifer R. Wood

Nebraska Beef Cattle Reports

Within the UNL physiology herd, a group of cows have been identified with excess androgen (androstenedione, A4) in their dominant follicle (30 fold higher than controls) and a 17% reduction in calving rate, suggesting subfertility. Th e objective was to identify altered granulosa cell gene expression that could be preventing these cells from converting excess androgen into estrogen. Microarray analysis suggests these granulosa cells experience inhibited proliferation resulting in a reduced total population of cells. Improved understanding of the causes of this phenotype may provide beef producers with tools to identify potentially subfertile cattle and improve reproductive efficiency.

Effect Of Dam Age On Offspring Productivity, Aline G. Da Silva, Jacqueline A. Musgrave, John Nollette Nollette, Andy Applegarth, Rick N. Funston

Nebraska Beef Cattle Reports

Records collected from 1997 to 2014 were analyzed to evaluate the effects of dam age on off spring productivity. Steer calves born from young mothers (2 and 3 yr old dams) had lighter carcasses with more carcasses grading Choice and upper 2/3 Choice. Th e calving performance and reproductive performance in the second breeding season for heifer calves born from first calving dams were the lowest compared with heifers born from multiparous dams. A quadratic effect of dam age on off spring performance was observed; as dam age increased, off spring performance increased, until dam age reached 7 to 8 …

Impact Of Supplementing Cattle With Omnigen- Af At The Receiving Or Finishing Phase On Beef Shelf- Life, Katherine I. Domenech, Michael D. Chao, Joe Buntyn Buntyn, Ty B. Schmidt, Chris R. Calkins

Nebraska Beef Cattle Reports

A patented proprietary product, OmniGen- AF, was evaluated to extend beef steak shelf- life at 8, 22 and 29 days of aging. Th e three treatment groups included a control group with no supplementation, OmniGen- AF supplementation for28 d aft er receiving, and supplementation for 210 d during finishing. Th e inclusion of OmniGen- AF had no effect on tenderness or visual discoloration under retail display conditions and minimal effects on fatty acid composition. Although color stability seemed to be unaffected by the supplementation, feeding OmniGen- AF throughout the entire feeding period tended to decrease oxidation. Supplementing cattle with a …

Effects Of Replacing A Traditional Growing Diet With A Complete Pelleted Feed On Total Tract Digestibility Of Growing Diets, Jana L. Harding, Melissa L. Jolly, Galen E. Erickson Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

A digestion study evaluated the effects of replacing traditional growing diets containing 60% untreated corn stover \or 60% alkaline treated corn stover, with one of two types of complete pelleted feeds (Pellet C or Pellet S) containing alkaline treated corn stover and dry distillers grains. Pellet C and S were harvested using different methods. Pellet S had the greatest OM and NDF digestibility, while the untreated stover had the lowest. Pellet C and the treated stover fell intermediate. Replacing a traditional growing diet with a complete pelleted feed resulted in similar or improved diet digestibility, depending on stover harvesting method.

Evaluating Syngenta Enhanced Feed Corn On Finishing Cattle Performance And Carcass Characteristics, Marie E. Harris, Melissa L. Jolly- Breithaupt, Brandon L. Nuttelman Nuttelman, Dirk Burken, Galen E. Erickson, Ivan G. Rush, Matt K. Luebbe

Nebraska Beef Cattle Reports

Two experiments were conducted to compare Syngenta Enhanced Feed Corn™ containing an alpha amylase enzyme trait (SYT- EFC) with commercially available corn grain without the alpha amylase enzyme trait (Conventional) for cattle performance and carcass characteristics at 2 locations. In Exp. 1, steers were fed SYT- EFC or Conventional corn with or without the addition of 25% Sweet Bran, or a BLEND (Conventional and SYT- EFC) without Sweet Bran. In Exp. 2, steers were fed SYT- EFC, Conventional, BLEND, or Conventional with an alpha amylase enzyme supplement (NZ). In Exp. 1, feed conversion improved 8.5% for SYT- EFC compared with …

Effect Of Harvest Method On Residue Quality, Tasha M. King, Robert G. Bondurant, Jana L. Harding Harding, Jim C. Macdonald, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A growing study was conducted to evaluate the effect of residue quality due to harvest method and inclusion of supplemental rumen undegradable protein on performance of growing steers. The residue harvested with an alternative method to minimize stem increased gain and improved efficiency compared to conventionally harvested corn residue. Inclusion of rumen undegradable protein increased gain and improved feed effi ciency compared to diets without supplemental rumen undegradable protein.

Finishing Yearling Heifers Using Self- Fed Dried Distillers Grains On Pasture, Jared M. Mracek, Jason M. Warner, Bradley R. Wetovick Wetovick, Dennis E. Bauer, Twig T. Marston, Jim C. Macdonald, Jerry D. Volesky, Richard J. Rasby

Nebraska Beef Cattle Reports

A 2- yr study compared a traditional system of grazing yearlings followed by a grain- based drylot finishing program to a system using a self- fed dried distillers grain supplement during a spring/summer/fall pasture grazing season. Th e self- fed (SF) heifers had greater ADG and ending BW on pasture but the traditionally managed heifers had greater final BW and HCW. At harvest, SF heifers had greater 12th rib back fat. When data were adjusted to a common empty body fat, carcass weight and marbling score were greater for traditionally managed heifers.

Effect Of Safeguard® On Fecal Egg Count And Steer Performance In Newly Received Calves, Antonio J. Neto, Curtis J. Bittner, Brandon L. Nuttelman Nuttelman, Galen E. Erickson

Nebraska Beef Cattle Reports

The effects of Safeguard® on fecal egg count (FEC) and performance of newly received calves in the feedlot were evaluated. Treatments were Safeguard® and Dectomax® injectable or only Dectomax® injectable. Th e basal diet consisted of 30% dry- rolled corn, 36% Sweet Bran® Cargill, 30% alfalfa hay, and 4% supplement. Th ere were no diff erences in DMI, ADG, F:G, and initial FEC between treatments. However, FEC on d 19 was lower for animals receiving Safeguard® and Dectomax® compared to Dectomax®. Th e combination of Safeguard® and Dectomax ® reduced FEC to very low amounts of newly received calves in …

How Many Clean-Up Bulls Are Needed Aft Er Estrus Synchronization And Artificial Insemination?, Hazy R. Nielson, Rick N. Funston

Nebraska Beef Cattle Reports

To evaluate the ideal number of bulls to use following estrus synchronization and artificial insemination (AI), research reporting AI and final pregnancy rates and bull to female ratio in Bos taurus cattle was reviewed and summarized. Pregnancy rate means were weighted based on the number of females in each study. Final pregnancy rates for a normal bull to female ratio (1:20 to 30) in a natural service setting were 87.8%. In comparison, final pregnancy rates following estrus synchronization and AI for a normal, intermediate (1:31 to 49), and half the number of bulls (1:50 to 60) were 87.8, 82.6, and …

Effect Of Feeding De-Oiled Dry Distillers Grains Plus Solubles On Beef Oxidation, Color And Tenderness, Keni E.Z. Nubiato, Katherine I. Domenech, Galen E. Erickson Erickson, Chris R. Calkins

Nebraska Beef Cattle Reports

Cattle fed a de-oiled dry distillers grains plus solubles (DDGS) diet (50% DM basis) were compared to cattle fed a corn- based control diet to determine effects on discoloration, oxidation, color, and tenderness of beef aged for 2, 8, 14 and 21 days. Dietary treatment had no effect on tenderness. From the fourth day of retail display, beef from animals fed de-oiled DDGS had greater lipid oxidation and greater percentages of discoloration. Th e de-oiled DDGS treatment also showed greater discoloration aft er 21 days of aging. While feeding de-oiled DDGS did not impact lipid oxidation at 2, 8 and …

Effects Of Direct- Fed Microbial Supplementation In Different Diets On Performance And Carcass Characteristics Of Beef Feedlot Heifers, Laura F. Prados, Nirosh D. Aluthge, Curtis J. Bittner Bittner, F. Henry Hilscher, Samodha C. Fernando, Galen E. Erickson

Nebraska Beef Cattle Reports

The objective of this study was to evaluate performance and carcass characteristics of heifers fed a newly developed direct- fed microbial (DFM), using 336 heifers in a pen study. Th e experiment consisted of feeding corn (CON) or 40% modified distillers grains plus solubles (40MDGS) and presence or absence of DFM added as a top- dress. No significant differences were observed for heifer performance and carcass characteristics due to DFM. Feeding MDGS increased ADG, while reducing F:G compared to CON. The DFM developed for this study did not enhance performance as was hypothesized, while feeding MDGS did.

Observations Of Forage Quality And Calf Gain When Grazing Double Cropped Forage Following Wheat Harvest, Kristen M. Ulmer, Robert G. Bondurant, Jana L. Harding Harding, Gary Lesoing, M. E. Drewnoski, James C. Macdonald

Nebraska Beef Cattle Reports

A 2- year cover crop grazing study was conducted following wheat harvest to evaluate the quality and yield of a 5- way forage annual forage mix in addition to cattle performance. In 2013, gain of steer calves was 2.03 lb/day, while 2014 steer calves had gain of 1.55 lb/day. Above ground forage production was 1.08 and 1.72 tons/acre in 2013 and 2014, respectively. Daikon radish and purple top turnip (brassicas) were high in sulfur and low in fiber. Fall forage production of brassica and oat based mixes following wheat grain harvest provide 97 to 137 lb of gain per acre.

Effect Of Pelleted Feed Products And Bambermycins On Performance When Fed To Cattle Grazing Residue, Cody A. Welchons, Robert G. Bondurant, F. Henry Hilscher Hilscher, Jim C. Macdonald, Galen E. Erickson, Cody A. Nichols

Nebraska Beef Cattle Reports

Steer calves grazing non- irrigated corn residue were supplemented with a corn residue and by- product pellet at 0.3, 0.7, or 1.1% of BW. Th e, pellet was formulated to deliver either 0 or 10 mg/steer daily of bambermycins (Gainpro). Th ere was no interaction between bambermycins inclusion and level of supplementation. Likewise, there was no effect of bambermycins on ending BW or ADG. As level of supplement increased, both ending BW and ADG increased linearly.

A Life Cycle Analysis Of Land Use In Us Pork Production, Greg Thoma, Marty Matlock, Ben Putman, Jasmina Burek

Food Systems

The goal of this study was to analyze land use in the production of US pork using Life Cycle Assessment (LCA). LCA is a comprehensive methodology for quantitatively analyzing potential environmental impacts associated with complex systems. Identification of processes contributing to high environmental impacts often highlights opportunities for gains in efficiency, which can increase the profitability and sustainability of US pork. The environmental impact category analyzed in this assessment was land use. After reviewing existing information regarding land use in agriculture and livestock production, analysis for US pork production was performed at two scales: cradle-to-grave and cradle-to-farm gate. The cradle-to-grave …

Genetic Parameters For Docility, Weaning Weight, Yearling Weight, And Intramuscular Fat Percentage In Hereford Cattle, J. A. Torres-Vázquez, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Cattle behavior, including measures of docility, is important to beef cattle producers not only from a human safety perspective but also due to potential correlations to economically relevant traits. Field data from the American Hereford Association was used to estimate genetic parameters for chute score (CS; n = 25,037), weaning weight (WW; n = 24,908), yearling weight (YW; n = 23,978), and intramuscular fat percentage (IMF; n = 12,566). Single-trait and bivariate animal models were used to estimate heritabilities and genetic correlations. All models included fixed effects of sex and contemporary group, defined as herd–year–season, and direct genetic and residual …

Production And Economic Efficiencies Of Intensified Cow–Calf Production Management Systems, Jason M. Warner

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of four experiments conducted was to evaluate production and economic efficiencies of intensified cow-calf production management systems. The first experiment tested the effect of calf age at weaning on cow and calf performance and feed utilization at 2 locations. Body weight change from early to conventional weaning time was greater for early-weaned cows. Cow BCS and conception rates were not impacted by weaning. Calf BW at conventional weaning time was greater for conventionally-weaned calves than early-weaned calves at ARDC, but greater for early-weaned than conventionally-weaned calves at PHREC. Calf ADG per unit of total feed energy intake was …

Failure Of A Heterologous Recombinant Sca5/Ompb Protein-Based Vaccine To Elicit Effective Protective Immunity Against Rickettsia Rickettsii Infections In C3h/Hen Mice, Sean P. Riley, Marissa M. Cardwell, Yvonne G. Chan, Ludovic Pruneau, Fabio Del Piero, Juan J. Martinez

Faculty Publications

Spotted fever group (SFG) rickettsial species are obligate intracellular tick-borne pathogens that are responsible for important human diseases. Previous reports have demonstrated the feasibility of using recombinant surface cell antigen Sca5/OmpB to elicit protective immunity against homologous challenges using murine models of Mediterranean spotted fever and Rocky Mountain spotted fever. In addition, the feasibility of generating cross-protective immunity against related rickettsial species has also been established, but the molecular basis for these phenomena was not explored. Here, we demonstrate that vaccination of C3H/HeN mice with a recombinant OmpB domain derived from Rickettsia conorii induced high titer humoral immune responses that …

Performance, Body Temperature And Blood Metabolites Of Feedlot Steers As Influenced By Environmental Conditions And Supplementation Of Zilpaterol Hydrochloride, Bradley M. Boyd

Department of Animal Science: Dissertations, Theses, and Student Research

Trial one was conducted at the United States Meat Animal Research Center (MARC) near clay center, NE during the summer of 2014. The objective of this trial was to measure the effects of supplementing zilpaterol hydrochloride (ZH) for the final 21 days of the finishing period, and shade, on performance, body temperature, respiration rate, and mobility of finishing beef steers. Feeding ZH increased hot carcass weight, dressing percent, longissimus muscle area, and reduce USDA yield grade. Shade did not affect steer performance and did not reduce body temperature. Zilpaterol hydrochloride increased respiration rate when compared to control cattle and had …

Genomic Analysis Of Sow Reproductive Traits: Identification Of Selective Sweeps, Major Genes, And Genotype By Diet Interactions, Melanie D. Trenhaile

Department of Animal Science: Dissertations, Theses, and Student Research

Reproductive traits, such as litter size and reproductive longevity, are economically important. However, selection for these traits is difficult due to low heritability, polygenic nature, sex-limited expression, and expression late in life. Marker-assisted selection may provide an alternative to increase genetic progress.

Nebraska Index Line (NIL) has been selected for litter size related traits since 1981. It is one of the main contributing lines to the UNL reproductive longevity resource population (n > 1,500), which was genotyped for 60,000 SNPs, phenotyped for age at puberty (AP), lifetime number of parities (LTNP), litter size traits, and other reproductive traits, and fed …

Dietary Lecithin Supplementation Can Improve The Quality Of The M. Longissimus Thoracis, Darryl N. D'Souza, Bronwyn L. Blake, Ian H. Williams, Bruce P. Mullan, Dave Pethick, Frank R. Dunshea

Animal production and livestock research articles

Forty crossbred (Large White × Landrace × Duroc) female pigs (16.4 kg ± 0.94 kg) were used to investigate the effect of dietary lecithin supplementation on growth performance and pork quality. Pigs were randomly allocated to a commercial diet containing either 0, 3, 15 or 75 g lecithin/kg of feed during the grower and finisher growth phase. Pork from pigs consuming the diets containing 15 g and 75 g lecithin/kg had lower hardness (P < 0.001) and chewiness (P < 0.01) values compared to the controls. Dietary lecithin supplementation at 75 g/kg significantly increased (P < 0.05) the linoleic acid and reduced (P < 0.05) the myristic acid levels of pork compared to the control and the 3 g/kg and 15 g/kg lecithin supplemented treatments. Pigs fed the 75 g/kg lecithin supplemented diet had lower plasma cholesterol (P < 0.05) at slaughter compared to pigs fed the control diet and the 3 g/kg and 15 g/kg lecithin supplemented treatments. These data indicate that dietary lecithin supplementation has the potential to improve the quality attributes of pork from female pigs.

Effects Of Shade And Feeding Zilpaterol Hydrochloride To Finishing Steers On Performance, Carcass Quality, Heat Stress, Mobility, And Body Temperature, Bradley M. Boyd, Steven D. Shackelford, Kristin E. Hales, Tami M. Brown-Brandl, Meredith L. Bremer, Matthew L. Spangler, T. L. Wheeler, D. A. King, Galen Erickson

Department of Animal Science: Faculty Publications

Steers (n = 480; 22% with black hides and 78% with red hides) were used to study the effects of shade and feeding zilpaterol hydrochloride (ZH) on performance, carcass quality, heat stress, mobility, and body temperature (BT). A randomized block design with a 2 × 2 factorial treatment arrangement was used with 4 replicates per treatment. Factors included housing type (open or shaded pens) and the feeding of ZH (0 or 8.33 mg/kg DM) the last 21 d on feed with a 3-d withdrawal. Cattle were blocked by BW into a heavy or light block and randomly assigned to pen …