Bioresource and Agricultural Engineering Commons^™

Nitric Oxide Sensors For Biological Applications, Nicole M. Iverson, Eric M. Hofferber, Joseph A. Stapleton

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Nitric oxide (NO) is an essential signaling molecule within biological systems and is believed to be involved in numerous diseases. As a result of NO’s high reaction rate, the detection of the concentration of NO, let alone the presence or absence of the molecule, is extremely difficult. Researchers have developed multiple assays and probes in an attempt to quantify NO within biological solutions, each of which has advantages and disadvantages. This review highlights many of the current NO sensors, from those that are commercially available to the newest sensors being optimized in research labs, to assist in the understanding and …

Evaluation Of Critical Nitrogen And Phosphorus Models For Maize Under Full And Limited Irrigation Conditions, Koffi Djaman, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Proper nitrogen (N) fertiliser application rates and timing of application, coupled with optimum irrigation management can improve the sustainability of maize production and reduce the risk of environmental contamination by nutrients. The impact of full and limited irrigation and rainfed conditions on in-season maize (Zea mays L.) shoot biomass nutrient concentration and critical N and phosphorus (P) indices were evaluated using a combination of measured nutrients and critical N and P models in south central Nebraska in 2009 and 2010. Four irrigation treatments [fully-irrigated treatment (FIT), 75% FIT, 60% FIT and 50% FIT) and rainfed] were imposed. Irrigation regimes …

Phenotypic Responses To Interspecies Competition And Commensalism In A Naturally Derived Microbial Co-Culture, Nymul Khan, Yukari Maezato, Ryan S. Mcclure, Colin J. Brislawn, Jennifer M. Mobberley, Nancy Isern, William B. Chrisler, Lye Meng Markillie, Brett M. Barney, Hyun-Seob Song, William C. Nelson, Hans C. Bernstein

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL- 58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We …

Impact Of Vegetative Treatment Systems On Multiple Measures Of Antibiotic Resistance In Agricultural Wastewater, Lisa M. Durso, Daniel N. Miller, Christopher G. Henry

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Wastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and animals, there remain large knowledge gaps surrounding applied details on the types and amounts of ARB/G associated with different agricultural wastewater treatment options and different ag production systems. This study evaluates a vegetative treatment system (VTS) built to treat the wastewater from a beef cattle feedlot. Samples were collected for three years, and plated on multiple media types to …

Special Issue: Microbial Community Modeling: Prediction Of Microbial Interactions And Community Dynamics, Hyun-Seob Song

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Microbial communities are networks of species, the interaction structure of which dynamically reorganizes in a varying environment. Even in a static condition, community dynamics are often difficult to predict due to highly nonlinear interspecies interactions. Understanding the fundamental principles of microbial interactions is therefore key for predicting and harnessing community function and properties. As extensively reviewed previously, mathematical models and computational methods that can predictively link interactions to community behaviors are indispensable tools for achieving this goal [1].

Impacts Of Cover Crops On Soil Physical Properties: Field Capacity, Permanent Wilting Point, Soil-Water Holding Capacity, Bulk Density, Hydraulic Conductivity, And Infiltration, Suat Irmak, Vivek Sharma, Ali T. Mohammed, Koffi Djaman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Field experiments were carried out to quantify the effects of cover cropping on soil physical properties. Field capacity (FC), permanent wilting point (PWP), soil-water holding capacity (SWHC), bulk density (  b), saturated and unsaturated hydraulic conductivity (Ks and Kus, respectively), and infiltration rates were measured and compared for four land cover treatments [cover crop without seed maize (CC), seed maize followed by cover crop (SCCC), bare soil, and seed maize without cover crop (SC)] in three large-scale production fields (~64 ha each) with silt loam soil in the 2012-2013, 2013-2014, 2014-2015, and 2015-2016 cover crop growing seasons. All production …

Three-Dimensional Modeling Of Nitrate-N Transport In Vadose Zone: Roles Of Soil Heterogeneity And Groundwater Flux, Simin Akbariyeh, Shannon L. Bartelt-Hunt, Daniel D. Snow, Xu Li, Zhenghong Tang, Yusong Li

Department of Civil and Environmental Engineering: Faculty Publications

Contamination of groundwater from nitrogen fertilizers in agricultural lands is an important environmental and water quality management issue. It is well recognized that in agriculturally intensive areas, fertilizers and pesticides may leach through the vadose zone and eventually reach groundwater. While numerical models are commonly used to simulate fate and transport of agricultural contaminants, few models have considered a controlled field work to investigate the influence of soil heterogeneity and groundwater flow on nitrate-N distribution in both root zone and deep vadose zone.

In this work, a numerical model was developed to simulate nitrate-N transport and transformation beneath a center …

Field-Based Scoring Of Soybean Iron Deficiency Chlorosis Using Rgb Imaging And Statistical Learning, Geng Bai, Shawn Jenkins, Wenan Yuan, George L. Graef, Yufeng Ge

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Iron deficiency chlorosis (IDC) is an abiotic stress in soybean that can cause significant biomass and yield reduction. IDC is characterized by stunted growth and yellowing and interveinal chlorosis of early trifoliate leaves. Scoring IDC severity in the field is conventionally done by visual assessment. The goal of this study was to investigate the usefulness of Red Green Blue (RGB) images of soybean plots captured under the field condition for IDC scoring. A total of 64 soybean lines with four replicates were planted in 6 fields over 2 years. Visual scoring (referred to as Field Score, or FS) was conducted …

Unmanned Aerial Systems-Based Remote Sensing For Monitoring Sorghum Growth And Development, Sanaz Shafian, Nithya Rajan, Ronnie Schnell, Muthukumar Bagavathiannan, John Valasek, Yeyin Shi, Jeff Olsenholler

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Unmanned Aerial Vehicles and Systems (UAV or UAS) have become increasingly popular in recent years for agricultural research applications. UAS are capable of acquiring images with high spatial and temporal resolutions that are ideal for applications in agriculture. The objective of this study was to evaluate the performance of a UAS-based remote sensing system for quantification of crop growth parameters of sorghum (Sorghum bicolor L.) including leaf area index (LAI), fractional vegetation cover (f_c) and yield. The study was conducted at the Texas A&M Research Farm near College Station, Texas, United States. A fixed-wing UAS equipped with a …

Rapeseed Seedling Stand Counting And Seeding Performance Evaluation At Two Early Growth Stages Based On Unmanned Aerial Vehicle Imagery, Biquan Zhao, Jian Zhang, Chenghai Yang, Guangsheng Zhou, Youchun Ding, Yeyin Shi, Dongyan Zhang, Jing Xie, Qingxi Liao

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The development of unmanned aerial vehicles (UAVs) and image processing algorithms for field-based phenotyping offers a non-invasive and effective technology to obtain plant growth traits such as canopy cover and plant height in fields. Crop seedling stand count in early growth stages is important not only for determining plant emergence, but also for planning other related agronomic practices. The main objective of this research was to develop practical and rapid remote sensing methods for early growth stage stand counting to evaluate mechanically seeded rapeseed (Brassica napus L.) seedlings. Rapeseed was seeded in a field by three different seeding devices. A …

Measuring The Influence Of Bedding On Soil Nitrogen Losses And Corn Crop Nitrogen Characteristics For Fall Applied Solid Beef Cattle Manure In Eastern South Dakota, Mukesh Mehata

Electronic Theses and Dissertations

Nitrogen (N) is a major component of chlorophyll which plays a key role in the photosynthesis process in crops. The N is one of the highest demanded nutrients by all plants for their growth and reproduction. Manure or inorganic fertilizer is often applied to fulfill the crops’ N demand. However, the applied N sources have the potential of N losses in different forms from the soil volume in many ways such as ammonia (NH₃) volatilization, aerial nitrous oxide (N₂O) loss, nitrate (NO₃ --N) leaching, and runoff and/or erosion. Soil fertility, crop yield, water quality, and …

A Quantitative Environmental Assessment Of Incorporating Torrefaction Into Farming Enterprises In Eastern South Dakota, Dinesh Fuyal

Electronic Theses and Dissertations

The use of renewable energy sources has been increasing in the recent years due to population growth and environmental concerns. Biomass is a promising energy source that can be used to produce biofuels or torrefied pellets. Torrefied biomass may be used in power plants, industrial and residential heating, feedstocks for gasification, air and water filtrating, and soil amendment. The interest of torrefied pellets as energy sources for various applications has been increased in the recent years due to the concerns about energy security and environmental issues. This study focuses on the economic and environmental assessment of agricultural feedstocks like corn …

Evaluating E. Coli Particle Attachment And The Impact On Transport During High Flows, Louis Amegbletor

Electronic Theses and Dissertations

Fecal indicator bacteria, including E. coli, are the leading cause of water quality impairments within assessed waters in the United States. The source of E. coli includes WWTP, leaking sewers, animal manure, wildlife, livestock, and stream bed sediment. Storm events contribute to bacteria loading within waters through wash-in of land sources of bacteria and resuspension of bacteria within sediments. Bacteria introduced into the water column are either attached to particles or are unattached (or free-living). The goal of this study was to examine the attachment of E. coli to different particle sizes, including their impact on contributing to water …

Development And Testing Of An Agricultural Nozzle Clog Detection Device Prototype, Chin Nee Vong

Student Theses and Dissertations

Nozzle clogging during spray application results in poor weed management and increased cost in re-spraying. A device that can detect nozzle clogging during the spraying operation is necessary. A novel method and device for detecting clogged nozzles that is externally attachable to each nozzle on the sprayer’s boom has been developed in the Precision Application Technology Lab at Arkansas State University. The study’s objectives include developing prototypes of this device and comparing the spray droplet size and spray pattern in controlled condition and spray coverage in field condition with and without using the device. The research demonstrated that this new …

A Risk-Based Method For Estimating The Carbon Sequestration Budget For A Mixed Hardwood Forest, Erin Cooper

Chemical Engineering Undergraduate Honors Theses

Carbon Dioxide (CO₂) levels in the atmosphere have risen from approximately 310 ppm in the 1950’s to over 400 ppm as of 2015. This rise in CO₂ has likely resulted in the observed warming trend of the earth’s atmosphere in the same time frame, causing significant concern in the scientific community. Several mitigation strategies have arisen to combat the upward trend of CO₂ emissions in recent decades, among them being carbon sequestration- the process of capturing CO₂ from the atmosphere and holding it for an extended period of time. This study used the U.S. Forest …

Design And Development Of An Electronic Granular Urea Applicator As An Attachment With Rice Transplanter, Md Towfiqur Rahman

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

In rice production system, transplanting and fertilizer application are tedious, time consuming and laborious task, and mostly done manually in Bangladesh. Mechanical planting of rice and granular urea placement have distinctive advantages over manual operation. Mechanical planting of rice becoming popular in the country in recent years and few machines have been developed for guti-urea deep placement, however having some limitations. Considering precise guti-urea placement along with rice transplanting, an attempt was under taken to design and fabricate an electronic control guti-urea applicator to be attach with a 4-row walk behind type rice transplanter. Fabrication of the electronic guti-urea applicator …

Performance Assessment Of Electromagnetic Soil Water Sensors In Different Soil Textural, Temperature, And Salinity Conditions, Jasreman Singh

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Determination of accurate and continuous measurements of volumetric water content (θ_v) is extremely valuable for irrigation management and other agronomic decisions. Lately, electromagnetic (EM) sensors are being widely used to monitor θ_v continuously which also offer the benefits of ease of installation, fewer regulatory and safety concerns, and cost effectiveness. However, the accuracy of parameters [soil temperature, electrical conductivity (EC_a), dielectric permittivity (ε_ra), and θ_v] reported by EM sensors need to be evaluated for them to be utilized for agricultural water management. In the current study, the accuracy of a wide …

High Throughput Phenotyping Of Sorghum For The Study Of Growth Rate, Water Use Efficiency, And Chemical Composition, Piyush Pandey

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Plant phenotyping using digital images has increased the throughput of the trait measurement process, and it is considered to be a potential solution to the problem of the phenotyping bottleneck. In this study, RGB images were used to study relative growth rate (RGR) and water use efficiency (WUE) of a diverse panel of 300 sorghum plants of 30 genotypes, and hyperspectral images were used for chemical analysis of macronutrients and cell wall composition. Half of the plants from each genotype were subjected to drought stress, while the other half were left unstressed. Quadratic models were used to estimate the shoot …

Thermodynamic Limitations To Agricultural Productivity And Food Security: Livestock In Sub-Saharan Africa, Calvin Harman

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

By 2050, to feed a nearly tripling and more urbanized population in sub-Saharan Africa (SSA) will require significant increases in crop productivity throughout the region’s agricultural systems, if a greater reliance on imports is to be avoided. Increases in crop yields to maximum potential productivity (closure of crop yield gaps) can produce more calories and protein, but may be insufficient to sustain the burgeoning human population according to recent analysis (van Ittersum et al., 2016). In this thesis, alternative management options (i.e. crop allocation and crop substitutions) are found to increase total energy productivity and the thermodynamic efficiency of food …

Cultivation Of Chlorella Sorokiniana Using Beef Feedlot Runoff Holding Pond Effluent, Mitchell Klaus Goedeken

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

A study was conducted to test the suitability of utilizing beef feedlot runoff holding pond effluent for cultivating algae. The algae strain used, Chlorella sorokiniana, was previously identified as a potential energy feedstock for cattle. The previous research was initiated in pursuit of a goal to develop a cycle of utilizing nutrients from beef manure to cultivate algae and then utilizing dewatered algae as a feed supplement for beef cattle.

Runoff holding pond effluent samples were collected from commercial beef production operations in Nebraska during spring 2016. Equal portions of samples from each cooperating farm were composited and then aliquoted …

Use Of Long Term Weather Data And Spatially Delineated Field Attributes To Predict Water And Energy Conservation From Variable Rate Irrigation, Sahil Sharma

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The declining levels of the Ogallala aquifer calls for more judicious use of water. Studies have shown that VRI has the potential for water savings. But adoption of VRI is still very low. The major reason is lack of information on the returns from the VRI systems and its feasibility in different fields. Also, a quantification of the required reduction in prices of VRI is necessary. So, an economic return analysis of VRI strategies was done to compare it to uniform irrigation management (UIM) using a water balance model based on long term weather data and field properties for a …

Setback Distance Requirements For Removal Of Swine Slurry Constituents In Runoff, John E. Gilley, Shannon L. Bartelt-Hunt, Kent M. Eskridge, Xu Li, Amy M. Schmidt, Daniel D. Snow

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The use of setback distances for manure application on cropland areas adjacent to surface water bodies could serve a function similar to vegetative filter strips. However, little information currently exists to identify the setback distances necessary to effectively reduce the transport of contaminants in runoff. The objective of this study was to determine the effects of setback distance and runoff rate on concentrations of selected constituents in runoff following land application of swine slurry to a no-till cropland area in southeast Nebraska. The study site had a residue cover of 7.73 Mg ha-1 and a slope gradient of 4.9%. The …

Quantifying Effectiveness Of Streambank Stabilization Practices On Cedar River, Nebraska, Naisargi Dave, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Excessive sediment is a major pollutant to surface waters worldwide. In some watersheds, streambanks are a significant source of this sediment, leading to the expenditure of billions of dollars in stabilization projects. Although costly streambank stabilization projects have been implemented worldwide, long-term monitoring to quantify their success is lacking. There is a critical need to document the long-term success of streambank restoration projects. The objectives of this research were to (1) quantify streambank retreat before and after the stabilization of 18 streambanks on the Cedar River in North Central Nebraska, USA; (2) assess the impact of a large flood event; …

Morphological, Thermal And Oxygen Barrier Properties Plasticized Film Polylactic Acid, Kurniawan Yuniarto, Bruce A. Welt, Foliatini -, Hanafi -, Candra Irawan

Journal of Applied Packaging Research

Introducing plasticizer poly(ethylene glycol) (PEG400) was applied into poly(lactic acid) (PLA) to produce film matrix packaging by direct casting. Non mechanical properties were carried out plasticized PLA including morphology, crystallinity structure and degree, thermal properties and oxygen barrier properties. Plasticized PLA revealed improving surface structure of PLA film matrix form fractures and homogenous film were achieved at 5% PEG 400. Chromatogram PLA and plasticized PLA were categorized crystal structure an α-form crystal. Intercalated and exfoliated structure did not occur significantly due to dispersion PEG 400 in the matrix but indicated dispersion structure. Thermal properties did not improve plasticized PLA significantly …

Both Adhe And A Separate Nadph-Dependent Alcohol Dehydrogenase Gene, Adha, Are Necessary For High Ethanol Production In Thermoanaerobacterium Saccharolyticum, Tianyong Zheng, Daniel G. Olson, Sean J. Murphy, Xiongjun Shao, Liang Tian, Lee Lynd

Dartmouth Scholarship

Thermoanaerobacterium saccharolyticum has been engineered to produce ethanol at ∼90% theoretical yield and titer of 70 g/L. Its ethanol-producing ability has drawn attention to its metabolic pathways, which could potentially be transferred to other organisms of interest. Here we report that the iron-containing AdhA is important for ethanol production in the high-ethanol strain of T. saccharolyticum (LL1049). A single-gene deletion of adhA in LL1049 reduced ethanol production by ∼50%, whereas multiple gene deletions of all annotated alcohol dehydrogenases except adhA and adhE did not affect ethanol production. Deletion of adhA in wild-type T. saccharolyticum reduced NADPH-linked ADH activity (acetaldehyde-reducing) by …

Performance Results Of Using Waste Vegetable Oil Based Biodiesel Fuel In A Turboshaft Gas Turbine Engine, Donald Bartlett 5218591, Blaine M. Heisner

ASA Multidisciplinary Research Symposium

The purpose of this research is to record and compare the operating parameters of a turboshaft gas turbine engine using two different blends of biodiesel fuel and the standard Jet A fuel.

An Optimal Milk Production Model Selection And Configuration System For Dairy Cows, Fan Zhang

PhDs

Milk production forecasting in the dairy industry has been an independent research topic since the early 20th century. The accurate prediction of milk yield can benefit both the processor (creameries) and the producer (dairy farmer) through developing short-term production schedules, planning long-term road maps, facilitating trade and investment in the dairy industry, improving business operations, optimising the existing infrastructure of the dairy industry, and reducing operating costs. Additionally, due to the innate characteristics of the milk production process, the accurate prediction of milk yield has been a challenging issue in the dairy industry. With the abolishment of EU milk quotas …

Development Of Cardiac Atrial Kick Shear Wave Elastography To Assess Myocardial Stiffness, Aaron J. Engel

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Increased myocardial stiffness, which is characteristic of many cardiovascular diseases, leads to a loss of diastolic function and is a cause of diastolic heart failure. Current methods to estimate myocardial stiffness rely on pressure-volume relationships derived from invasive cardiac catheter measurements. Noninvasive methods to estimate myocardial stiffness include ultrasound-based and magnetic resonance-based shear wave elastography, where the stiffness of the tissue is related to the propagation speed of a shear wave. Currently, ultrasound-based cardiac shear wave elastography includes acoustic radiation force based methods; however, the in vivo generation and detection of the shear waves in myocardium is significantly degraded due …

Techno-Economic Implications Of Fed-Batch Enzymatic Hydrolysis, Ellen Emanuel

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

As ethanol production continues to increase in the United States, cellulosic ethanol continues to gain traction as a viable option for meeting ethanol demands. In this work, a literature review of techno-economic analyses for cellulosic ethanol was conducted. It was found that pretreatment methods greatly affect the ethanol production costs. There is a lack of techno-economic data comparing a batch enzymatic hydrolysis to a fed-batch enzymatic hydrolysis for a separate hydrolysis and fermentation production process. Consequently, a techno-economic analysis comparing cellulosic ethanol production using batch versus fed-batch enzymatic hydrolysis was conducted. SuperPro Designer software (Intelligen, Scotch Plains, New Jersey), a …

Evaluation Of Physical - Chemical Contaminants In Lumbardhi River In Prizren For The Period May - June 2017, Gashi Sami, Bajmaku Ymerr, Besar Veseli, Drini Petrit

UBT International Conference

This paper covers the monitoring of water pollution in the river basin from the source to the White Drin flow monitoring the river flow both in the rural and urban areas of water quality. The biggest problem in environmental protection is the quality of surface water. The results from the analysis of the study will serve to know the pollution from these physical-chemical parameters and at the same time to manage the eco-status in order to prevent negative trends and create a desirable balancing of so important the ecosystem as it is the system consist of Lumbardhi River, with biodiversity …