Bioresource and Agricultural Engineering Commons^™

High-Throughput Robotic Ethanol Inhibition Assays For Engineered Thermophilic Biofuel Strains, Kevin He, Daniel Olson, Marybeth Maloney, Anthony Lanahan

Wetterhahn Science Symposium Posters

Ethanol stress assays are commonly used to evaluate microbial tolerance, metabolic adaptation, and fermentation performance. However, manual liquid handling introduces variability across replicate wells and small-volume pipetting steps, limiting reproducibility and throughput. This study developed an automated OT-2 robotic workflow to generate replicated ethanol concentration gradients for high-throughput inhibition assays in engineered thermophilic biofuel strains. Kinetic plate-reader measurements were used to quantify ethanol-dependent growth responses under anaerobic fermentation conditions. The reasearch question is: How do engineered thermophilic biofuel strains differ in ethanol-dependent growth inhibition under anaerobic fermentation conditions, and can automated robotic assays improve the reproducibility of these measurements? Can …

Ammonia Synthesis By Nanosecond Pulsed Atmospheric Pressure Plasma Jets Impinging On Water, Zach Caudell, Lynnet Rich, Olga Pakhomova, Chunqi Jiang

Bioelectrics Publications

Developing energy-efficient technologies for carbon-neutral ammonia (NH₃) synthesis is critical for decentralized fertilizer production and global decarbonization. This study investigates generating NH₃ from water using a nanosecond pulsed atmospheric pressure plasma jet (ns‑APPJ) operating in either N₂ or dry air. The plasma jet reactor employed approximately 250 ns, up-to-22 kV pulses at 500 Hz to sustain a nonequilibrium discharge impinging directly on static liquid water. The kinetics, energy efficiency, and product selectivity of NH3 formation were quantified as functions of the pulse voltage, repetition frequency (PRF), and gas flow rate. NH₃ production increased linearly with treatment time and scaled strongly …

Assessment Of Soybean Response To Irrigation Variability In Eastern Nebraska Using The Aquacrop Model, Anmol Singh, Saleh Taghvaeian, Yufeng Ge, Derek M. Heeren, Frank Bai

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Soybean [Glycine max (L.) Merr.] is a major irrigated crop in eastern Nebraska, where recent expansions in irrigated agriculture and projected trends in irrigation demand necessitate thorough investigations of approaches that can optimize its irrigation management. In this study, the AquaCrop model was calibrated and validated using 19 variable irrigation treatments from a five-year field experiment. The model accurately simulated canopy cover, soil water content, and grain yield, with validation normalized root mean square error (nRMSE) of 12%, 7%, and 7%, respectively. The model was subsequently applied to estimate soybean yield and water requirement during a 15-year period (2010–2024) …

An Integrated Modeling Framework To Evaluate Circularity Of The Corn–Water–Ethanol–Beef Nexus, Heydi Han

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation examines the transition from conventional linear agricultural and food bioenergy systems toward more circular, resilient, and resource-efficient configurations. Prior research indicates that spatial and temporal variability in soil, weather, animal biological responses, and management decisions strongly influences productivity, nutrient-use efficiency, and profitability. Studies have also shown that a diversified circular system can buffer these sources of variability, improving stability and adaptive capacity. Building on these insights, the central focus of this work is to develop an integrated modeling framework to evaluate circularity within the Corn-Water-Ethanol-Beef (CWEB) nexus and explore how climate variability and management choices shape system-wide performance. …

Locations Of Outfalls In The Indian River Lagoon And Lake Worth Lagoon, Florida, Kelly M. Kibler, Melinda J. Donnelly, Vincent Encomio, Madison Giuntoli, Manisha Thenuwara, Brice Bennett, Laura Andrade Barron, Mariam Sonbol, Wynter Raine Seppala, Namritha Ramakrishnan, Jennifer Jordan Báez, Beth Orlando, Irene Arpayoglou, Melissa Meisenburg

Indian River Lagoon Shorelines

The spatial dataset: UCF_Outfalls_IRL_LWL.shp was created under grants from the Indian River Lagoon National Estuaries Program and Florida Sea Grant. These projects cumulatively led to creation of a living shoreline restoration prioritization model and hydrodynamic habitat suitability models for 1,550 km (963 miles) of estuarine shorelines in Indian River Lagoon (IRL) and Lake Worth Lagoon (LWL).

The dataset presented here includes the location of outfalls identified throughout the study region and general characteristics of the shoreline where they were identified.

Seagrass Persistence In The Indian River Lagoon And Lake Worth Lagoon, Florida, Kelly Kibler, Melinda Donnelly, Vincent Encomio, Madison Giuntoli, Manisha Thenuwara, Brice Bennett, Laura Andrade Barron, Mariam Sonbol, Wynter Raine Seppala, Namritha Ramakrishnan, Jennifer Jordan Báez, Beth Orlando, Irene Arpayoglou, Melissa Meisenburg

Indian River Lagoon Shorelines

The spatial dataset: UCF_seagrasspersistence_IRL_LWL.shp was created with support from the Indian River Lagoon National Estuaries Program and Florida Sea Grant. These projects cumulatively led to creation of a living shoreline restoration prioritization model and hydrodynamic habitat suitability models for 1,550 km (963 miles) of estuarine shorelines in Indian River Lagoon (IRL) and Lake Worth Lagoon (LWL). The dataset presented herein represents persistence of seagrass throughout waters of the IRL and LWL.

Living Shoreline Prioritization And Hydrodynamic Habitat Suitability Models For Restoration In The Indian River Lagoon And Lake Worth Lagoon, Florida, Kelly M. Kibler, Melinda J. Donnelly, Vincent Encomio, Madison Giuntoli, Manisha Thenuwara, Brice Bennett, Laura Andrade Barron, Mariam Sonbol, Wynter Raine Seppala, Namritha Ramakrishnan, Jennifer Jordan Báez, Beth Orlando, Irene Arpayoglou, Melissa Meisenburg

Indian River Lagoon Shorelines

The spatial dataset: UCF_shorelinemodel_IRL_LWL.shp, was created with support from the Indian River Lagoon National Estuaries Program and Florida Sea Grant. These projects cumulatively led to creation of a living shoreline restoration prioritization model and hydrodynamic habitat suitability models for 1,550 km (963 miles) of estuarine shorelines in Indian River Lagoon and Lake Worth Lagoon.

The dataset presented herein includes:

1. shoreline data from >15,000 transects collected in the field,
2. shoreline wave climate data created through hydrodynamic modeling and frequency analysis of historic data,
3. categorization of potential risk for shoreline boat wake impact,
4. persistence of seagrass within 210 m of the shoreline, …

Evaluation Of Performance Characteristics And Groundwater Contamination Risks Associated With On-Farm Swine Carcass Disposal Via Composting And Shallow Burial With Carbon, Gustavo Castro Garcia

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation evaluates the performance of three on-farm swine carcass disposal methods: whole carcass composting (WCC), ground carcass composting (GCC), and shallow burial with carbon (SBC), focusing on biosecurity factors and groundwater contamination risks. Foreign animal diseases (FADs), such as African swine fever and classical swine fever, pose severe economic and animal well-being risks if introduced to the United States. With confirmation of an FAD, swine movement will be halted, creating an urgent need for practical, biosecure, and environmentally responsible on-farm disposal strategies for swine carcasses. Nebraska, as a leading swine-producing state, exemplifies the vulnerability of high-density livestock regions to …

Groundwater Flooding On Highways In The Nebraska Sandhills: Applying Remote Sensing, Precipitation And Groundwater Modeling To Determine Depth, Cause And Frequency, Aaron R. Mittelstet

Nebraska Department of Transportation: Research Reports

Climate change significantly impacts infrastructure sustainability, particularly through shifts in precipitation patterns, intensity, and duration. These precipitation dynamics may increase the occurrence of surface water and groundwater flooding. Groundwater in the Nebraska Sand Hills (NSH), including the thickest portions of the High Plains Aquifer, extends close to the land surface in interdunal areas, making the NSH vulnerable to groundwater flooding. Recent events involving heavy precipitation, snow melt, and rising groundwater levels have caused prolonged highway flooding, disrupting transportation networks. This study estimates groundwater flood inundation depth, duration and frequency in the highways of NSH using remote sensing techniques and groundwater …

Geophysical Tools For Agricultural Management: Trends, Challenges, And Opportunities, Sophia Becker, Trenton Franz, Yufeng Ge, Joe Luck, Derek M. Heeren

School of Natural Resources: Faculty Publications

Agrogeophysics is a convergence of proximal soil sensing and hydrogeophysics in which geophysical methods are used to describe soil patterns and dynamics that shape agronomic management (including precision agriculture). To fully realize its potential for optimizing agricultural production, current barriers and targets for future agrogeophysics research must be identified. In this review, we compare the trajectory of agrogeophysics within peer-reviewed literature, patents, and the US university extension literature and examine the current challenges and opportunities for supporting agricultural production and sustainability with agrogeophysical tools. We conducted a repeatable search of Web of Science and Scopus for seven geophysical methods (electromagnetic …

Ai Tools In Natural Resources And Environmental Systems For The Classroom, Research, Extension And Industry, Derek M. Heeren, C. T. Agouridis, Debabrata Sahoo, Isaya Kisekka, Trisha L. Moore

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This white paper synthesizes a series of panel insights on AI adoption across education, Extension, research and industry contexts within natural resources and environmental systems. It emphasizes that while advanced machine learning and generative AI introduce structural shifts in autonomy, scale, and cognitive augmentation, their value lies in augmenting, not replacing, human expertise. Applied tool examples include decision‑support systems for rural water operators, HAB‑diagnosis apps integrating image classification and natural‑language guidance, an irrigation educational chatbot tailored to user background, and sensor‑driven orchard AI for light interception estimation and data gap filling. A recurring design imperative involves co‑development with end users, …

Response Of Soybean To Variable Irrigation Levels In Eastern Nebraska Using The Aquacrop Model, Anmol Singh

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

AquaCrop was calibrated and validated for soybean [Glycine max (L.) Merr.] using 19 irrigation treatments from five years using data from field experiments. The model accurately simulated canopy cover (CC), soil water content (SWC), and grain yield, with overall validation nRMSE values of 12%, 7%, and 7%, respectively. The overall validation nRMSE for SWC in individual 30-cm soil layers was comparatively higher, at 19%. The model was subsequently applied for long-term simulations (2010–2024) to estimate soybean yield and water requirements for two dominant soil types, Yutan silty clay loam and Tomek silt loam under three irrigation levels - rainfed, …

Optimizing Nitrogen Management: Assessing Nitrate Transport In The Deep Vadose Zone And Its Impact On Groundwater Quality In East-Central Nebraska, Muili O. Lawal

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Groundwater contamination by nitrate (NO₃-N) is a growing environmental and public health concern driven largely by intensive agricultural fertilizer applications. The vadose zone plays an important role in NO₃-N transport, influencing groundwater quality and agricultural sustainability. This study investigated NO₃-N leaching dynamics in the deep vadose zone of Water Quality Sub-Area 30 (WQA30) within the Lower Loup Natural Resources District (LLNRD), northeast of Columbus, Nebraska (mean groundwater NO₃-N: 20.3 mg/L), using soil cores from 16 shallow (to 6.1 m) and four deep (down to 25.9 m) across four zones, two with inorganic fertilizer (Zones 1 and 4) and two with …

Private Property Vs. Public Access: Socio-Engineering Framework For Managing Non-Meandering Waters In South Dakota’S Prairie Pothole Region, Lydia Loken, Sushant Mehan

The Journal of Undergraduate Research

This study addresses the growing importance of balancing private ownership and public interest of Non-Meandering Waters (NMWs) overlaying private property in the South Dakota Prairie Pothole Region (PPR) through both a legal and socio-engineering lens. This study aims to develop an integrated management framework for Non-Meandering Waters in the South Dakota Prairie Pothole Region that combines water allocation principles, the Public Trust Doctrine, and water quality objectives to guide policy decisions, ensuring equitable access, long-term sustainability, and a balanced approach to both public and private interests. Data collection from October 2024 through February 2025 and included a literature review of …

In-Season Nitrogen Mmanagement: Leveraging Data Visualization For Precision Agriculture, Chathurika Harshani Narayana

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Effective nitrogen management is vital for sustainable agriculture, impacting both crop yield and environmental health. Traditional methods often use fixed application rates set before planting, which do not adapt to changing crop needs during the season. This can lead to over- or under-application, reducing efficiency and sustainability. While modern tools like sensors, satellites, and UAVs provide valuable real-time data on crop and field conditions, integrating and using this data to guide timely nitrogen decisions remains a major challenge. In-season nitrogen management offers a solution by allowing for dynamic adjustments to nitrogen applications, addressing crop needs as they arise. This approach …

Crop2cloud Platform: Real-Time Data Integration For Agricultural Water Monitoring, Bryan Nsoh, Abia Katimbo, Kendall Dejonge, Wei-Zhen Liang, Hongzhi N. Guo, Yufeng Ge, Derek M. Heeren, Yeyin Shi, Xin Qiao, Daran R. Rudnick, Hope Njuki Nakabuye, Birru Girma, Isa Kabenge, Joshua Wanyama

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Efficient water management is vital for sustainable agriculture, yet integrating real-time data for precise irrigation remains a challenge. This study designed the Crop2Cloud (C2C) platform, a system that leverages advanced sensors using Internet of Things (IoT), edge and cloud computing techniques, and computed Water Stress Indices (WSIs) and machine learning models (i.e., fuzzy logic), to provide scalable and real-time irrigation decisions. The C2C platform aggregates several data including Volumetric Water Content (VWC) from TDR sensors (Acclima Inc., US) installed at four multiple depths, canopy temperatures (Tc) measured by Infrared Radiometers (IRTs) (Apogee Instruments, US), as well as weather information and …

Cover Crops Can Reduce Greenhouse Gas Emissions From No-Till Maize In Southern Brazil: Insights From A Long-Term Field Experiment, Guilherme Rosa Da Silva, Adam J. Liska, Cimélio Bayer

Adam Liska Papers

Brazil is one of the countries that has the most agricultural area under no-till (NT) management. This research study aims to evaluate life-cycle greenhouse gas (GHG) emissions from maize (M) grain production in agroecosystems that used different cover crops under NT management in southern Brazil. The data for this study were from a long-term 41-year field experiment in southern Brazil. The long-term experiment evaluated the effects of fallow (F) and cover crops (oat (O), vetch (V), cowpea (B), pigeon pea (P), and lablab (L)) on nitrous oxide and methane emissions and soil carbon (C) sequestration in maize agroecosystems. Five cropping …

Impact Of Irrigation Systems On Water Use And Water Productivity: A Case Study Using Aquacrop In Khartoum, Sudan, Abdalhakam Almagzoop

Department of Agricultural and Biological Systems Engineering: Masters Project Reports

Water scarcity significantly challenges agricultural productivity in arid regions like Khartoum, Sudan. This study evaluates the impact of three irrigation systems—furrow, sprinkler, and surface drip—on the water use and productivity of sorghum (Sorghum bicolor L.) under different climatic conditions using the AquaCrop model. Simulations were conducted for three representative years: a wet year (2022), a normal year (2015), and a dry year (2018). The results demonstrate that irrigation substantially improved water use efficiency and crop yield compared to non-irrigated (rainfed) conditions. Rainfed crops resulted in lower biomass, harvest index, and yield, particularly in the dry year, highlighting the importance …

Enhancing Water Sustainability In North Africa: Literature Review And Synthesis Of Current Knowledge Gaps In Sudan, Osman M. A. Adam

Department of Agricultural and Biological Systems Engineering: Masters Project Reports

This study delves into the critical role of groundwater in addressing global water challenges, with a focus on the Nubian Sandstone Aquifer System (NSAS) in north Africa. Groundwater constitutes a source of potable water, irrigation, and industrial use, especially in arid regions where surface water is limited. We analyzed the status of water quantity, withdrawals, recharge, and geological characteristics in the NSAS, specifically in Sudan, Egypt, and Libya. Though the NSAS is largely an untapped resource, water withdrawals from the NSAS in Egypt, Sudan, and Libya are projected to increase substantially from 2000 to 2100 due to population growth, with …

Harvesting Modulation In Three Species Food Chains Using A Robust Control Approach, Hector Puebla, Mariana Rodriguez-Jara, Priti Kumar Roy

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Excavated Tanks (Farm Dams), Department Of Primary Industries And Regional Development, Western Australia

Natural resources factsheets

Guidelines and information for planning, legal requirements, design, construction and management of excavated tanks for water storage on farm.

Excavated tanks (farm dams) provide effective water storage wherever surface water run-off can be harvested for livestock, crop spraying, irrigation and domestic use on rural properties.

Excavated tanks for rainfall storage are common in the southern agricultural areas of WA.

Well-designed and constructed dams are farm assets, safe and require little maintenance.

Poor design and construction can lead to poor water harvesting and storage, excessive costs, downstream erosion and risks to property and people.

DPIRD recommends that excavated tanks should be …

A Step By Step Shoreline Attribute Analysis For Selected Waterbodies In The Gulf Of Mexico To Promote The Use Of Living Shorelines, Christoper Boyd, Xutong Niu, Taylor R. Horn

Journal of Extension

Living Shorelines are being promoted by coastal extension professionals as a more resilient nature-based solution to control shoreline erosion. The Virginia Institute of Marine Sciences Living Shorelines Suitability Model was run in selected waterbodies within the Gulf of Mexico.

The locations of the selected water bodies, coastal data sets used, and shoreline protection recommendations generated by the Model are presented. A step-by-step statistical analysis conducted through ArcGIS Pro from these selected coastal shorelines will illustrate how extension professionals with novice GIS experience can use the model output to promote living shorelines to coastal property owners, city managers, and developers.

Integration Of Matlab And Machine Learning To Accelerate Evaluation Of Biological Activity In Agricultural Soils And Promote Soil Health Improvement Goals, Andrew Stiven Ortiz Balsero

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Traditionally, assessments of soil biological activity have been confined to laboratory settings, creating a disconnect with practical in-field methods. To bridge this gap, cotton fabric degradation has been used to illustrate soil microbial activity under different management practices. While effective, these demonstrations are subjective and labor-intensive.

Researchers have explored using image processing software like ImageJ and Adobe Photoshop to streamline this process. Although these tools accurately quantified fabric degradation under varying soil conditions, the methods remained labor-intensive and complex. Consequently, these methods were still not ideal for on-farm use by agricultural practitioners.

To further address labor and complexity limitations, the …

The Growth Of Ethanol In Brazil: Sugarcane, Corn, And A Few Other Things, Fabio Mattos

Cornhusker Economics

Although we can expect more ethanol produced from corn in the coming years, it is unclear whether it will keep expanding at the same pace as in recent years or how much it will contribute to total ethanol production.

One point to keep an eye on is the typical dynamics of markets. If supply and demand conditions and price movements make corn-ethanol more profitable, ethanol plants in Brazil will keep increasing their production. If it makes more economic sense to produce corn-ethanol (as it happened recently), they will use more corn to produce ethanol.

Supply and demand conditions for ethanol, …

Enhancing Water Sustainability In North Africa: Literature Review And Synthesis Of Current Knowledge Gaps In Sudan, Osman M. A. Adam, Aaron R. Mittelstet, Derek M. Heeren, Troy E. Gilmore

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This study delves into the critical role of groundwater in addressing global water challenges, with a focus on the Nubian Sandstone Aquifer System (NSAS) in North Africa. Groundwater constitutes a source of potable water, irrigation, and industrial use, especially in arid regions where surface water is limited. We analyzed the status of water quantity, withdrawals, recharge, and geological characteristics in the NSAS, specifically in Sudan, Egypt, Libya, and Chad. Though the NSAS is largely an untapped resource, we evaluated various scenarios to determine the quantity of cropland that can be sustainably irrigated. The NSAS is located in an arid region, …

Investigating The Impact Of Secondary Activation Time On The Adsorption Properties Of Activated Biochar For Arsenic Removal From Wastewater, Achyuth Bharadwaj Rayarao

Graduate Theses and Dissertations (2019 - present)

Biochar, a naturally occurring and renewable carbon material, becomes porous through activation. Activated biochar is an ideal candidate for use as an adsorbent. The objective of this study is to investigate whether activated biochar derived from Colorado soft pine serves as a better adsorbent and demonstrates enhanced adsorption capabilities after undergoing secondary physical activation at various activation times. The effect of secondary action time on physical adsorption properties drawn from the analysis of isotherm plots, surface areas and pore size patterns were examined by using a physisorption analyzer. Chemical structures were identified using both ultimate and proximate analyses, while porosity …

Will Saf Turbocharge The Corn Ethanol Market?, Richard K. Perrin, Lilyan E. Fulginiti, Felipe Miranda De Souza Almeida

Cornhusker Economics

The long-run outlook for the corn ethanol industry is questionable, due to a transition to electric and hybrid vehicles. One source of hope for the long run is the potential demand for producing Sustainable Aviation Fuel (SAF). SAF is a key component in the United States Aviation Climate Action Plan, a path to net-zero greenhouse gas (GHG) emissions in the aviation industry by 2050. Demand for ethanol for SAF offers hope to the ethanol industry, but it depends a great deal on policy decisions that are being made now. Here we sketch out this story.

Based on the information available …

Field Research Report: Results From The Enreec Vri Field For The 2021, 2022, And 2023 Crop Seasons, Derek M. Heeren, Ali T. Mohammed, Eric Wilkening, Christopher M. U. Neale, Alan L. Boldt, Ankit Chandra, Precious Nneka Amori, Ivo Z. Goncalves, Yeyin Shi, Guillermo R. Balboa

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Long-term irrigation management research has been conducted from 2014 to 2023 for corn and soybean at the Eastern Nebraska Research, Extension, and Education Center (ENREEC) Variable Rate Irrigation (VRI) Field located in subhumid east-central Nebraska (in the Lower Platte North Natural Resources District). The objective of this report was to present the overall results from the VRI Field for 2021 to 2023. Across the three growing seasons, there were the following irrigation treatments: Best Management Practice (BMP), 50% BMP, 125% BMP, rainfed, Spatial ET Modeling Interface (SETMI), SDD1, SDD2, machine-learning-based Cyber-Physical System (CPS), a student team recommended rate, and industry …

The Efficacy Of Wetland Treatment Systems Used To Treat Runoff Mixtures From Different Landscapes Across Kentucky, Emily Nottingham

Theses and Dissertations--Biosystems and Agricultural Engineering

The overarching goal was to improve our understanding of the cumulative effect of increased use and occurrence of commonly detected contaminants of potential concern (CPCs) on nitrogen (N) transformation processes in wetlands. The central hypothesis was that those various mixtures of nutrients and CPCs entering treatment wetlands impact N removal pathways. The first study assessed the current state of the literature on the implications to N removal in wetlands in the presence of pesticides and antibiotics. 181 primary studies were identified. Removal efficiencies for nitrate varied widely across the studies, with CPCs impacting microbial communities. A knowledge gap remains in …

Stage And Discharge Prediction From Documentary Time-Lapse Imagery, Kenneth W. Chapman, Troy E. Gilmore, Mehrube Mehrubeoglu, Christian D. Chapman, Aaron R. Mittelstet, John E. Stranzl Jr.

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Imagery from fixed, ground-based cameras is rich in qualitative and quantitative information that can improve stream discharge monitoring. For instance, time-lapse imagery may be valuable for filling data gaps when sensors fail and/or during lapses in funding for monitoring programs. In this study, we used a large image archive (> 40,000 images from 2012 to 2019) from a fixed, ground-based camera that is part of a documentary watershed imaging project (https://plattebasintimelapse.com/). Scalar image features were extracted from daylight images taken at one-hour intervals. The image features were fused with United States Geological Survey stage and discharge data as …