Bioresource and Agricultural Engineering Commons^™

Specific Salt Effects On The Formation And Thermal Transitions Among Β-Lactoglobulin And Pectin Electrostatic Complexes, Stacey Ann Hirt

Open Access Theses

Factors of ion specificity and ionic strength (I~0-100) were studied in the electrostatic complex formation and protein particle formation by thermal treatment for a β-lactoglobulin and pectin system. ζ-potential showed β-lactoglobulin and pectin began to interact near pH 5.50 and interactions were strengthened with decrease in pH. Visible light turbidimetry and light scattering at 90° revealed a trend in critical pH transitions for electrostatic complex formation based on both the ionic strength and the anion of the salt species, while effects of the monovalent cation was insignificant. Critical pH values for complex formation and separation (pHc and pHΦ) decreased with …

Rheological Properties Of Gluten Free Dough Systems, Stephany Aurea Tandazo

Open Access Theses

Bread is the one of the oldest processed foods and a major wheat based product. The basic process involves mixing of ingredients until the flour is converted into dough, followed by baking the dough into a loaf. A very important step in breadmaking is to know how to make good quality dough. However, the increasing knowledge of people being diagnosed with celiac disease (gluten intolerance) has encouraged scientists to develop healthier and better quality gluten-free products that would greatly improve the quality of life of celiac patients. The main objective of this study was to create a dough system composed …

Chemical Weathering And Mobility Of Inorganic Species In Dry Disposed Ash: An Insight From Geochemical Fractionation And Physicochemical Analysis, S.A. Akinyemi, W.M. Gitari, A. Akinlua, L.F. Petrik

Coal Combustion and Gasification Products Journal Archive

The burning of low-grade coal produces vast amounts of ash and other solid by-products. In South Africa, coal combustion by-products undergo disposal on land as dry heaps or slurried to dams. In this study, the geochemical partitioning, transport, and mobility of elements in dry disposed ash dumps were investigated using a modified sequential extraction scheme. The chemical and mineralogical compositions of 50 drilled core samples were investigated by X-ray fluorescence (XRF), X-ray diffraction (XRD), and inductively coupled plasma mass spectrometry. Ternary plots of major elements as determined by XRF showed that the 15-year-old and 2-week-old dry disposed ashes are sialic, …

2013 Nebraska Groundwater Quality Monitoring Report

Nebraska Department of Environmental Quality: Reports

The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …

Evaluation Of Chemical Additives For The Separation And Recovery Of Bacteria From Food Matrices, Jennifer Leanne Frederick, Stephen P. Walker, Czarena L. Crofcheck, Melissa Newman, Frederick Payne

Biosystems and Agricultural Engineering Faculty Publications

The microbiological testing of foods is a well-established science. Due to the severity of foodborne pathogen illnesses, the widespread use and implementation of rapid detection methods in food testing labs is increasingly important. The first step for successful testing is sampling. Surfactants have been widely used in food microbiology, but there is not much, if any, published research about the use of fatty alcohols and chemical dispersants as aids in microbial separation and recovery. The microbial extraction efficiency of Escherichia coli K12 and Listeria innocua from three representative food matrices (hot dogs, spinach, and milk) was measured using chemical additives …

Alkaline Hydrogen Peroxide Pretreatment For Its Use In An On-Farm Bioprocessing Facility, Mary Kathryn Gray

Theses and Dissertations--Biosystems and Agricultural Engineering

Pretreatment is an essential step in biofuel production from lignocellulose. Disruption of the lignin structure gives enzymes and fermentation organisms access to long chains of cellulose and hemicellulose. For this project’s purposes, the pretreatment must work within the framework of an on-farm butanol bioprocessing facility. Alkaline hydrogen peroxide (AHP) is a delignification method that potentially provides several advantages. At the alkaline pH, powerful hydroxyl radicals are formed; which attack lignin. The objectives of this study were to determine if AHP removes substantial lignin for the feedstocks, corn stover, wheat straw, switchgrass and miscanthus, and to determine if AHP acts as …

Sodium Hydroxide Pretreatment Of Corn Stover And Subsequent Enzymatic Hydrolysis: An Investigation Of Yields, Kinetic Modeling And Glucose Recovery, Alicia Modenbach

Theses and Dissertations--Biosystems and Agricultural Engineering

Many aspects associated with conversion of lignocellulose to biofuels and other valuable products have been investigated to develop the most effective processes for biorefineries. The goal of this research was to improve the efficiency and effectiveness of the lignocellulose conversion process by achieving a more basic understanding of pretreatment and enzymatic hydrolysis at high solids, including kinetic modeling and separation and recovery of glucose.

Effects of NaOH pretreatment conditions on saccharide yields from enzymatic hydrolysis were characterized in low- and high-solids systems. Factors associated with pretreatment and hydrolysis were investigated, including duration of pretreatment at different temperatures and NaOH loadings, …

Hydrologic Characterization Of A Rain Garden Mitigating Stormwater Runoff From A Commercial Area, John T. Mcmaine

Theses and Dissertations--Biosystems and Agricultural Engineering

Impervious surfaces such as roads, sidewalks, and roofs increase the volume of runoff generated in a watershed. Traditional stormwater management techniques emphasize conveyance of runoff away from impervious surfaces in order to reduce flooding. Rain gardens are becoming popular as a different means to manage stormwater in such a way that runoff is captured and infiltrated onsite rather than conveyed offsite. A stormwater management system consisting of a rainwater harvest system, rain garden, and infiltration chamber was built at the Coca-Cola Refreshments USA, Inc. distribution center in Lexington, Kentucky during the fall of 2011. Precipitation, inflow, and water level were …

Nutrients Recycling Strategy For Microalgae-Based Co2 Mitigation System, Xinyi E

Theses and Dissertations--Biosystems and Agricultural Engineering

Coal-fired electricity production is the major emitter of CO₂ and other greenhouse gases including NO_x and SO_x. Microalgae-based CO₂ mitigation systems have been proposed to reduce the net CO₂ emission from coal-fired power plants. This study focused on developing an optimum culture media and exploring the possibilities for recycling nutrients, which were added as commercial mineralized chemicals at the beginning of cultivation.

In order to release the nutrients embedded in the cells so that they can be used as a nutrient source for new cells, Scenedesmus biomass was digested by anaerobic bacteria. Results showed …

Evaluation Of Handling Equipment Sound Pressure Levels As Stressors In Beef Cattle, Christina M. Lyvers

Theses and Dissertations--Biosystems and Agricultural Engineering

Sound pressure level (SPL) is known to cause stress in cattle but is often overlooked as a potential source of fear for cattle when designing handling equipment. Current literature does not offer guidelines for the design of equipment with regard to SPL. It is, however, recommended that handling equipment should be designed to minimize the SPL during handling. The purpose of this experiment was to measure stress levels in a group of cattle which were subjected to a series of varying sounds in order to determine a design threshold limit for handling equipment. Treatments included two frequencies, 1 kHz and …

Precise Evaluation Of Gnss Position And Latency Errors In Dynamic Agricultural Applications, Michael P. Sama

Theses and Dissertations--Biosystems and Agricultural Engineering

A method for precisely synchronizing an external serial data stream to the pulse-per-second (PPS) output signal from a global navigation satellite-based system (GNSS) receiver was investigated. A signal timing device was designed that used a digital signal processor (DSP) with serial inputs and input captures to generate time stamps for asynchronous serial data based on an 58593.75 Hz internal timer. All temporal measurements were made directly in hardware to eliminate software latency. The resolution of the system was 17.1 µs, which translated to less than one millimeter of horizontal position error at travel speeds typical of most agricultural operations.

The …

Using Gis To Delineate Headwater Stream Origins In The Appalachian Coal-Belt Region Of Kentucky, Jonathan A. Villines

Theses and Dissertations--Biosystems and Agricultural Engineering

Human activity such as surface mining can have substantial impacts on the natural environment. Performing a Cumulative Hydrologic Impact Assessment (CHIA) of such impacts on surface water systems requires knowing the location and extent of these impacted streams. The Jurisdictional Determination (JD) of a stream’s protected status under the Clean Water Act (CWA) involves locating and classifying streams according to their flow regime: ephemeral, intermittent, or perennial. Due to their often remote locations and small size, taking a field inventory of headwater streams for surface mining permit applications or permit reviews is challenging. A means of estimating headwater stream location …

Qualitative And Quantitative Analysis Of Lignocellulosic Biomass Using Infrared Techniques: A Mini-Review, Feng Xu, Jianming Yu, Tesfaye Tesso, Floyd Dowell, Donghai Wang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Current wet chemical methods for biomass composition analysis using two-step sulfuric acid hydrolysis are time-consuming, labor-intensive, and unable to provide structural information about biomass. Infrared techniques provide fast, low-cost analysis, are non-destructive, and have shown promising results. Chemometric analysis has allowed researchers to perform qualitative and quantitative study of biomass with both near-infrared and mid-infrared spectroscopy. This review summarizes the progress and applications of infrared techniques in biomass study, and compares the infrared and the wet chemical methods for composition analysis. In addition to reviewing recent studies of biomass structure and composition, we also discuss the progress and prospects for …

Evaluation Of Endogenous Allergens For The Safety Evaluation Of Genetically Engineered Food Crops: Review Of Potential Risks, Test Methods, Examples And Relevance, Richard E. Goodman, Rakhi Panda, Harsha Ariyarathna

Department of Food Science and Technology: Faculty Publications

The safety of food produced from genetically engineered (GE) crops is assessed for potential risks of food allergy on the basis of an international consensus guideline outlined by the Codex Alimentarius Commission (2003). The assessment focuses on evaluation of the potential allergenicity of the newly expressed protein(s) as the primary potential risk using a process that markedly limits risks to allergic consumers. However, Codex also recommended evaluating a second concern, potential increases in endogenous allergens of commonly allergenic food crops that might occur due to insertion of the gene. Unfortunately, potential risks and natural variation of endogenous allergens in non-GE …

Testing The Fuel Efficiency Of Tractors With Continuously Variable And Standard Geared Transmissions, Christopher N. Howard, Michael F. Kocher, Roger M. Hoy, Erin E. Blankenship

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A John Deere 8295R IVT tractor with a continuously variable transmission (CVT) and a John Deere 8295R PowerShift (PST) tractor with a standard geared transmission (GT) were tested for fuel consumption at three different travel speeds with six different load levels applied per speed. The JD 8295R PST tractor was tested both at full throttle (FT) and shifted up two gears and throttled back (SUTB) to achieve the same travel speed as at full throttle. For each travel speed with each transmission mode, fuel consumption was determined to be linearly related to drawbar power. Linear regression analyses were performed, and …

Use Of A Surrogate To Evaluate The Impact Of Tillage On The Transport Of Steroid Hormones From Manure-Amended Agricultural Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Mitiku Mamo, Shannon L. Bartelt-Hunt, Daniel D. Snow, David P. Shelton, David D. Tarkalson, Terry L. Mader, Simon J. Van Donk, Tian C. Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef feedlot manure distributed to row crop production areas is a potential surface water contaminant source of the steroid hormones commonly used in beef cattle production. This article reports on research conducted at the University of Nebraska Haskell Agricultural Laboratory near Concord, Nebraska, in July 2009. Manure, collected from beef feedlot pens, was stockpiled for ten months prior to application to a row crop field. Previous research identified that the detection frequency of steroid hormones in beef manure varies greatly. Thus, a surrogate (17α-ethynylestradiol, EE2) was applied at a rate of 75 g ha^-1 to ensure detectable concentrations in …

Maize Evapotranspiration, Yield Production Functions, Biomass, Grain Yield, Harvest Index, And Yield Response Factors Under Full And Limited Irrigation, Koffi Djaman, Suat Irmak, William R. Rathje, Derrel L. Martin, Dean E. Eisenhauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

South-central Nebraska is one of the most extensively irrigated areas in the U.S., with over 65,000 active irrigation wells, and maize is the major agronomical crop produced. Maize production in this region requires supplementary irrigation for maximum productivity. Effective on-farm implementation of full and limited irrigation practices for potential improvements of crop productivity requires knowledge of locally developed crop yield response to water functions. In this study, the effects of full and limited irrigation practices on maize (Zea mays L.) plant height, leaf area index (LAI), grain yield and biomass production, actual crop evapotranspiration (ET_a), yield production functions, …

Development And Evaluation Of Ordinary Least Squares Regression Models For Predicting Irrigated And Rainfed Maize And Soybean Yields, Vivek Sharma, Daran R. Rudnick, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Understanding the relationships between climatic variables and soil physical and chemical properties with crop yields on large scales is critical for evaluating crop productivity to make better assessments of local and regional food security, policy, land and water resource allocation, and management decisions. In this study, ordinary least squares(OLS) regression models were developed to predict irrigated and rainfed maize and soybean yields at the county level as a

function of explanatory variables [precipitation (P), actual crop evapotranspiration (ETa), organic matter content (OMC), cation exchange capacity (CEC), clay content (CC), and available soil water capacity (ASW)] of the dominant soil type …

Implementation Of A ”Rapid Design Challenge” In A Cross-Disciplinary Senior Capstone Course And Evaluation Of Device Performance, Abby M. Kelly, Austin Lammers, David D. Jones, Richard Stowell, Roger Hoy, Evan Curtis, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The senior capstone experience within the Department of Biological Systems Engineering at the University of Nebraska-Lincoln is a two-semester, two-course sequence intended to give senior students realistic design experience, working with real projects, real clients, faculty consultants, and teammates to produce a deliverable that meets the client’s needs. Students within this course sequence come from two different degree programs (agricultural engineering and biological systems engineering) and within each degree program from a variety of “emphasis areas”, e.g. biomedical engineering, environmental engineering, machine design. While the goal of this course is for students to experience a “real” design project, we felt …

Acetyl-Coa Carboxylases Are Checkpoints In Adipocyte Differentiation, Elizabeth Lyn Cordonier, Daniel Camara Teixeira, Zhonghi Han, Angela K. Pannier, Janos Zempleni

Department of Agricultural and Biological Systems Engineering: Faculty Publications

No abstract provided.

Network Analysis Of Endogenous Gene Expression Profiles After Polyethyleneimine-Mediated Dna Delivery, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background—DNA delivery systems, which transport exogenous DNA to cells, have applications that include gene therapy, tissue engineering and medical devices. Although the cationic nonviral DNA carrier polyethyleneimine (PEI) has been widely studied, the molecular factors and pathways underlying PEI-mediated DNA transfer remain largely unknown, preventing the design of more efficient delivery systems.

Methods—HEK 293 T cells were treated with polyplexes formed with PEI and pEGFPLuc encoding for green fluorescent protein (GFP). Transfected cells expressing GFP were flowseparated from treated, untransfected cells. Gene expression profiles were obtained using Affymetrix HG-U133 2.0 microarrays and differentially expressed genes were identified using R/ Bioconductor. …

Fate And Transport Of Antimicrobials And Antimicrobial Resistance Genes In Soil And Runoff Following Land Application Of Swine Manure Slurry, Stacy R. Joy, Shannon L. Bartelt-Hunt, Daniel D. Snow, John Gilley, Brian L. Woodbury, David B. Parker, David B. Marx, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Due to the use of antimicrobials in livestock production, residual antimicrobials and antimicrobial resistance genes (ARGs) could enter the environment following the land application of animal wastes and could further contaminate surface and groundwater. The objective of this study was to determine the effect of various manure land application methods on the fate and transport of antimicrobials and ARGs in soil and runoff following land application of swine manure slurry. Swine manure slurries were obtained from facilities housing pigs that were fed chlortetracyline, tylosin or bacitracin and were land applied via broadcast, incorporation, and injection methods. Three rainfall simulation tests …

Review Of Us And Eu Initiatives Toward Development, Demonstration, And Commercialization Of Lignocellulosic Biofuels, Venkatesh Balan, David Chiaramonti, Sandeep Kumar

Civil & Environmental Engineering Faculty Publications

Advanced biofuels produced from lignocellulosic biomass offer an exciting opportunity to produce renewable liquid transportation fuels, biochemicals, and electricity from locally available agriculture and forest residues. The growing interest in biofuels from lignocellulosic feedstock in the United States (US) and the European Union (EU) can provide a path forward toward replacing petroleum-based fuels with sustainable biofuels which have the potential to lower greenhouse gas (GHG) emissions. The selection of biomass conversion technologies along with feedstock development plays a crucial role in the commercialization of next-generation biofuels. There has been synergy and, even with similar basic process routes, diversity in the …

"Apps"—An Innovative Way To Share Extension Knowledge, Joseph S. Dvorak, Tanya C. Franke-Dvorak, Randy R. Price

Biosystems and Agricultural Engineering Faculty Publications

Extension professionals across the country are continuously seeking innovative ways to reach clientele and to disseminate timely, educational information. A new avenue to reach clientele includes the use of smartphone "apps." The "Machinery Sizing" app, which was developed to ease the estimation of tractor horsepower to implement sizing for Extension clientele anytime, anywhere, is explained as a key example for Extension professionals to utilize apps in disseminating information to clientele. There are many benefits to using apps, including information availability wherever Internet service is available on the smartphone, ease of computations of equations, and automatic updates being sent to users.

Design, Fabrication And Manufacturing Analysis Of A Rollover Protection System, Nikolas Paris

BioResource and Agricultural Engineering

This senior project discusses the design, fabrication and manufacturing analysis of a rollover protection system specifically designed for early body style Ford Broncos (1966-1977). The rollover protection system, or roll cage, is an integral safety feature on vehicles which commonly encounter extreme terrain conditions. Many vehicles which attempt to traverse terrain such as this are not always properly equipped with a rollover protection system. This is the case with the early body style Ford Bronco. This system will be designed to market the niche of early bronco parts.

Bioflocculation Of Wastewater Treatment Pond Suspended Solids, Louis Lefebvre

Master's Theses

Bioflocculation of Wastewater Treatment Pond Suspended Solids

Louis Lefebvre

Wastewater treatment lagoons and high rate algae ponds (HRAPs) can provide cost effective wastewater treatment, but they commonly have high effluent concentrations of total suspended solids (TSS). In this thesis algae pond effluent was treated in a beaker testing apparatus (mixed and aerated) with various mixtures of activated sludge and primary effluent simulating differing activated sludge aeration basin compositions then was allowed to settle to assess settleability. Conventionally, microalgal suspended solids are removed by chemical coagulation followed by separation methods that often have a high cost relative to the low cost …

Long-Term Trends In Air Temperature Distribution And Extremes, Growing Degree-Days, And Spring And Fall Frosts For Climate Impact Assessments On Agricultural Practices In Nebraska, Kari E. Skaggs, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Air temperature influences agricultural practices and production outcomes, making detailed quantifications of temperature changes necessary for potential positive and negative effects on agricultural management practices to be exploited or mitigated. Temperature trends of long-term data for five agricultural locations, ranging from the subhumid eastern to the semiarid western parts of Nebraska, were studied to determine local temperature changes and their potential effects on agricultural practices. The study quantified trends in annual and monthly average maximum and minimum air temperature (T_max and T_min), daily temperature range (DTR), total growing degree-days, extreme temperatures, growing-season dates and lengths, and …

Modernization Of House #3: Improving Greenhouse Cut Flower Production And Students' Experience By Modernizing House #3, Kristina Anton

Biomedical Engineering

This paper documents the modernization of the black cloth, lighting, shade, and support systems in one of Cal Poly's on-campus, student operated cut flower production greenhouses. Problems with the old system are examined along with design requirements for a new system. Alternate designs are considered along with the installation process of the chosen design.

Developing A Restorable Wetland Index For Rainwater Basin Wetlands In South-Central Nebraska: A Multi-Criteria Spatial Analysis, Zhenghong Tang, Xu Li, Nan Zhao, Ruopu Li, F. Edwin Harvey

Community and Regional Planning Program: Faculty Scholarly and Creative Activity

It is always challenging for decision makers to prioritize wetland conservation programs at the landscape scale. This study employed a GIS-based multi-criteria spatial decision support tool that identified locations with the highest restoration potential for wetland conservation programs in the Rainwater Basin in south-central Nebraska. Five indicators were considered to assess wetland restoration potential: (1) Vegetation characteristics; (2) Soil characteristics; (3) Water volume released from hydrological modification of agricultural irrigation pits; (4) Topographical depression status; and (5) Habitat condition. The results suggested 192 (1.6% of the total) hydric soil footprints as the highest prioritized locations for future wetland restoration programs. …

Initial Changes In Species Cover Following Savanna Restoration Treatments In Western Iowa, David A. Mckenzie, Thomas B. Bragg, David M. Sutherland

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

Study areas in the Iowa Loess Hills were used to evaluate short-term responses of understory species to three treatment methods designed to facilitate restoration of Quercus macrocarpa savanna. Treatments included burning alone, burning with thinning, and burning with clear-cutting. Plant abundance and diversity were compared before treatment and one year after treatment. Ninety-nine plant species were identified during the study, of which 40 were new following treatment, although most of these were forest associates. Increases in diversity of understory species were observed after treatment, particularly in plots with combined burning and thinning. The forb group was most consistent in response …