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Articles 32671 - 32700 of 5154054
Full-Text Articles in Entire DC Network
From Skilled Labor Pipeline To College-Prep Track: A Study Of Admissions, Access, And Intent In A Massachusetts Vocational High School, Jennifer Ferland
From Skilled Labor Pipeline To College-Prep Track: A Study Of Admissions, Access, And Intent In A Massachusetts Vocational High School, Jennifer Ferland
Educational Studies Dissertations
This mixed methods study examined the effectiveness and equity of vocational high school admissions in Massachusetts, focusing on an oversubscribed regional vocational high school and its largest urban comprehensive feeder school. Although vocational schools were created to provide equitable, high‑quality career and technical education, and to prepare students for skilled employment, most vocational students now pursue postsecondary academic pathways, contributing to shortages in the trades. At the same time, traditional admissions criteria—focused on grades, attendance, and behavior—have historically limited access for English Learners, students with disabilities, low‑income students, and students of color. The purpose of this study was to investigate …
From Anxiety To Resilience: A Case Study Of Female-Identifying Middle School Students At A Math Summer Camp, Elizabeth Teixeira
From Anxiety To Resilience: A Case Study Of Female-Identifying Middle School Students At A Math Summer Camp, Elizabeth Teixeira
Educational Studies Dissertations
Mathematics anxiety, defined as a fear that hampers engagement in mathematical tasks (Richardson & Suinn, 1972), affects nearly 50% of students (Johnston-Wilder et al., 2014) and has a disproportionate impact on female students (Devine et al., 2018; Stoet et al., 2016). Math anxiety is often treated in the research literature as a relatively stable trait. This perspective may not fully capture how anxiety manifests in specific moments of mathematical engagement or account for the role of environmental conditions that can both precipitate and mitigate anxious responses. In response to these gaps, this study investigates how mathematics anxiety manifests in …
Elementary Residents And Culturally Sustaining Pedagogy In Mathematics, Quayisha F. Clarke
Elementary Residents And Culturally Sustaining Pedagogy In Mathematics, Quayisha F. Clarke
Educational Studies Dissertations
This qualitative interpretive embedded case study examines how seven preservice elementary mathematics residents in a Northeastern teacher residency program identify and enact culturally sustaining pedagogy (CSP) (Paris and Alim, 2017) in their math instruction, and how their intersectional identities shape these enactments. Drawing on CSP, intersectionality, and discretionary spaces (Ball, 2018), the study explores how residents interpret, reason through, and make moment-to-moment decisions that affirm students’ learning in mathematics.
Data were collected across two semi-structured interviews, three classroom observations, post-observation debriefs, lesson plans, coursework artifacts, and a researcher's reflexive journal. Analysis followed a hybrid inductive–deductive approach, using thematic and axial …
Sing Without Even Light - How The Arts Inform And Enhance Learning, Creativity, Imagination, And Wellbeing In Elementary Classrooms In Kajiado, Kenya: Discovering Creative Confidence, Lindsay L. Stewart
Educational Studies Dissertations
Creativity and imagination can be felt, described, seen, and measured. In this study, this is achieved through a reflective process and awareness of creative practice and its potential. The process can aid and increase understanding of self, cognitive and creative process, social-emotional interactions, agency, and action, and reveals an individual’s developmental level of creative confidence.
A gap exists in student and teacher voice regarding their creative awareness and confidence. This study reveals how 29 children ages 8-15 and 14 teachers from Kajiado, Kenya, describe creativity and imagination, supporting both learning and wellbeing.
The study applies an Arts-Based Research approach, primarily …
Built For This: An Autoethonographic Study Of A Journey Through Leadership Rooted In Reflection, Resilience, And Reinvention, Weslie Etienne
Built For This: An Autoethonographic Study Of A Journey Through Leadership Rooted In Reflection, Resilience, And Reinvention, Weslie Etienne
Educational Studies Dissertations
This qualitative autoethnographic study examined how leadership was experienced, enacted, and sustained by a Black woman navigating district-level leadership within predominantly White institutional contexts. Grounded in Critical Race Theory, Intersectionality, and Black Feminist Thought, this research centered lived experience as a site of knowledge production and employed longitudinal journaling and artifact analysis to capture leadership development as a temporal, relational, and embodied process. The study was guided by three research questions: (1) How did race, gender, and institutional structures shape leadership development and experience? (2) How were leadership identities constructed, negotiated, and sustained through self-authored narratives? and (3) How could …
Nurtured Meaning: A Hermeneutic Phenomenological Study Of The Tree Of Life In Contemporary Women's Art, Christina A. Defilipp
Nurtured Meaning: A Hermeneutic Phenomenological Study Of The Tree Of Life In Contemporary Women's Art, Christina A. Defilipp
Educational Studies Dissertations
The Tree of Life has historically flourished across cultures as an archetypal image of interconnection, regeneration, and continuity. In the Anthropocene, however, its symbolic vitality is reframed by the realities of environmental crisis. This study investigates how contemporary women artists across the globe sustain and reinterpret the Tree of Life’s meaning to express themes of ecological, spiritual, and cultural relevance. Employing a qualitative design grounded in hermeneutic phenomenology, the study analyzes publicly available artworks and published reflections, including interviews, catalog essays, and artist statements. A purposive sample of twenty women artists from diverse cultural traditions forms the dataset, each represented …
"Free To Fall": Milton's Satan And The Trial Of Discernment, Mae Legare
"Free To Fall": Milton's Satan And The Trial Of Discernment, Mae Legare
English Honors Theses
William Blake once wrote, "Milton was of the Devil's party without knowing it," reasoning a deep-seated alignment with evil that only becomes illuminated in Satan's vibrantly humanized character. By analyzing Milton's earlier political prose, Areopagitica and The Tenure of Kings and Magistrates, alongside Satan's monologues in Paradise Lost, the author and his fallen characters' voices begin to sound synonymous. They are enraged by similar injustices and failures of political institutions, and they employ very similar methods of argumentation and political rhetoric. Blake was correct, in a sense, that Milton was "of the Devil's party," but I argue that …
The “Fallen Woman” Reconsidered: Inner And Outer Redemption In Victorian Literature, Caroline Kramer
The “Fallen Woman” Reconsidered: Inner And Outer Redemption In Victorian Literature, Caroline Kramer
English Honors Theses
This undergraduate thesis critically examines three female protagonists situated within the “fallen woman” character archetype of Victorian literature through a lens of redemption: Ruth Hilton in Ruth by Elizabeth Gaskell (1853), Mercy Merrick in The New Magdalen by Wilkie Collins (1873), and Tess Durbeyfield in Tess of the d’Urbervilles by Thomas Hardy (1891). While nineteenth-century readers may have expected that these women needed to undergo a moral transformation based on circumstances of their past, this thesis argues that none of them fundamentally require “redemption” according to any of the word’s typical definitions; rather, the question posed regards the modes through …
The Brief (Edition #46, May 2026), William & Mary Law School
The Brief (Edition #46, May 2026), William & Mary Law School
The Brief
No abstract provided.
Evaluation Of Soybean (Glycine Max (L.) Merr.) Response Following Simulated Damage, Brittany Rhea
Evaluation Of Soybean (Glycine Max (L.) Merr.) Response Following Simulated Damage, Brittany Rhea
Theses and Dissertations
Deer damage to row crops has been an ongoing issue in the state of Mississippi, and hail damage can be catastrophic. The objective of this research was to evaluate soybean response following simulated deer and hail damage at specific growth stages and damage levels. Soybean yield response to deer damage indicates the singular feedings of 75 % results in a loss of up to 2688.32 kg ha-1 in most scenarios, and continual feeding at the 50 % level leads to a yield reduction of up to 2168.81 kg ha-1 in all scenarios. Hail damage at V3 and R1 growth stages …
Evaluating The Spatial Relationship Between Geomorphology And Subsurface Structures On The Us Atlantic Margin With Applications To Other Terrestrial Planets, Andrea Caitlyn Stiles
Evaluating The Spatial Relationship Between Geomorphology And Subsurface Structures On The Us Atlantic Margin With Applications To Other Terrestrial Planets, Andrea Caitlyn Stiles
Theses and Dissertations
High-resolution digital elevation models (DEMs) are widely available for terrestrial planetary bodies, but subsurface structural data remain limited. This research evaluated whether surface geomorphology could predict subsurface features, specifically faults and methane seeps, along the US Atlantic continental margin as an analog site. Faults were mapped with seismic interpretation and compared with quantitative geomorphic classifications derived from 100-m bathymetry including slope, aspect, rugosity, bathymetric position index, and geomorphon classes. Maximum-entropy modelling was used to predict fault occurrences from surface data. Results indicated that specific geomorphic landforms and slopes had the strongest control on fault presence, reflected in topographic expression of …
Oxidized Lipids Are Inflammatory Danger Signals. The Hydrolytic Enzyme Carboxylesterase 1 Regulates Their Metabolic Fate, Thereby Shaping Macrophage Phenotype., Oluwabori Adekanye
Oxidized Lipids Are Inflammatory Danger Signals. The Hydrolytic Enzyme Carboxylesterase 1 Regulates Their Metabolic Fate, Thereby Shaping Macrophage Phenotype., Oluwabori Adekanye
Theses and Dissertations
Macrophages originate from hematopoietic progenitors they are immune cells, part of innate immunity. They patrol tissues to identify damage-associated molecular patterns (DAMPs) and pathogen-associated molecular patterns (PAMPs) from microorganisms. Macrophage pattern recognition receptors (PRRs) detect these signals, and respond by secreting lipid mediators, cytokines, and chemokines, thereby initiating the inflammatory response. Carboxylesterases (CES) belong to the α/β-hydrolase fold family of proteins and catalyze the hydrolysis of ester-containing substrates into alcohols and carboxylic acids. CES1 is one of the six CES identified in the human genome. They are localized in the endoplasmic reticulum lumen and cytosol of cells, primarily expressed in …
Improving Durability And Structural Integrity Of Southern Pine And Sweetgum Through Fabrication, Treatment, And Mechanical Testing., Ershad Ahmmed
Improving Durability And Structural Integrity Of Southern Pine And Sweetgum Through Fabrication, Treatment, And Mechanical Testing., Ershad Ahmmed
Theses and Dissertations
This research integrates material modification, preservative performance, and engineered fabrication to enhance the long-term durability and structural reliability of southern pine and sweetgum for outdoor and structural applications. The first project investigates the effects of simulated checks and splits on the strength and stiffness of southern pine fence posts. By introducing controlled defects and conducting mechanical testing, the study quantifies reductions in load-carrying capacity and evaluates how defect severity influences structural performance. The results provide insight into service life prediction and grading considerations for in-ground applications. The second project evaluates water ingress behavior in creosote-treated sweetgum using wetting angle measurements …
Community Development Professionals’ Perceptions Of Agritourism In Mississippi, Emma Jane Berry
Community Development Professionals’ Perceptions Of Agritourism In Mississippi, Emma Jane Berry
Theses and Dissertations
Agritourism has emerged as a strategy for supporting rural economies, strengthening community identity, and increasing agricultural literacy. Although prior research has examined agritourism operators and visitors, limited attention has been given to the perceptions of community development professionals who influence local planning and program support. The purpose of this qualitative exploratory study was to examine community development professionals’ perceptions of agritourism’s role in community development, economic growth, and agricultural literacy in Mississippi. Guided by Social Exchange Theory, data were collected through two semi-structured virtual focus groups with 12 professionals representing Extension, tourism, economic development, and Main Street organizations. Data were …
Characterization Of Water Quality In The Mississippi Sound Using In-Situ And Satellite Observations, William Elliott Butler
Characterization Of Water Quality In The Mississippi Sound Using In-Situ And Satellite Observations, William Elliott Butler
Theses and Dissertations
The Mississippi Sound is a highly productive estuary facing increasing pressures from natural disasters, oil spills, freshwater inflows, and coastal urbanization, yet it remains insufficiently monitored. In this study, key water quality parameters were quantified at 25 stations across the Mississippi Sound from 2024-2025 and integrated with Sentinel-3 satellite imagery retrievals to generate monthly turbidity maps from 2016-2025. Peak turbidity (~10 FNU) and TSS (~20 mg/L), reduced Secchi depth (~100 cm) and conductivity (~20,000 μS/cm), were observed from late winter to early summer; these conditions varied in fall and early spring. Colored dissolved organic matter was moderate, while chlorophyll-a and …
Assessment Of Energy And Hydration Demands Of Farm Workers In Mississippi, Mckenzie Lynne Hargrove
Assessment Of Energy And Hydration Demands Of Farm Workers In Mississippi, Mckenzie Lynne Hargrove
Theses and Dissertations
The agricultural community in Mississippi is critical to the state’s economy and the health of its communities. Farm workers meet these needs by performing highly demanding work with long hours of labor, limited resources, and stressful environmental conditions. The purpose of this investigation was to examine and measure indicators of physiological and behavioral adaptations to the heat such as whole body sweat rate (WBSR), sweat sodium (Na+) content, urine specific gravity (USG), and fluid intake, as well as determine total daily energy expenditure (TEE) and water influx (rH2O) via the doubly labeled water technique (DLW) in farm workers during the …
Developing And Evaluating U.S. Army Bridging For Ship Simulation, Jacob C. Hodges
Developing And Evaluating U.S. Army Bridging For Ship Simulation, Jacob C. Hodges
Theses and Dissertations
This research investigates the intricacies of ship simulation and developmental processes for integrating wet gap crossing capabilities into a simulated environment. The US Army Mark III Bridge Erection Boat (BEB) and Improved Ribbon Bridge (IRB) were modeled in commercial off the shelf (COTS) and Government Co-Owned (GCO) navigation software suites to determine their performance capabilities and limitations. Each model was constructed using naval architecture concepts to accurately capture their hydrostatic and hydrodynamic effects before being validated against available performance data and expert elicitation from expert US Army watercraft operators. Models were configured and tested in simulated river flows with current …
Root-Cause-Analysis Of Crane System Variable Frequency Drive Failures Through Testing And Power Quality Analysis, Elizabeth Hayes
Root-Cause-Analysis Of Crane System Variable Frequency Drive Failures Through Testing And Power Quality Analysis, Elizabeth Hayes
Theses and Dissertations
This study investigates the root-cause-analysis of repeated failures of an AC-DC converter in a variable frequency drive system on a 325-Ton crane through electrical testing based on NETA and IEEE standards. The testing is accompanied with a power quality study based on the IEEE 519-2019, simulation using SKM power modeling software, and fault study from two captured faults. The converter failures presented no immediate solution despite experienced technicians, engineers, and other specialists. This study attempts to eliminate root-cause by examining the line-side equipment and in-feed power quality as contributions to the converter faults.
Parhsom: A Novel Parallel Hierarchical Self-Organizing Map Implementation, Rebekah E. Lane
Parhsom: A Novel Parallel Hierarchical Self-Organizing Map Implementation, Rebekah E. Lane
Theses and Dissertations
The digital age has completely transformed the way that information is processed and stored, which makes cybersecurity a crucial field of research. Cybersecurity contains many different domains, but this work focuses on Intrusion Detection Systems (IDSs). Within the literature, Hierarchical Self-Organizing Maps (HSOMs) have been used to create trustworthy, explainable, and AI-based IDSs. However, HSOMs are trained sequentially, which means that training HSOMs on large datasets is slow. This work presents a novel parallel HSOM architecture, called parHSOM. The purpose of this research is to investigate the effect that parallel computation has on the HSOM training time. parHSOM is tested …
Assessment Of Local Ecological Knowledge, Oyster Predation, And Habitat Function To Improve Management Of Nearshore Habitats, Cynthia Evelyn Marie Lupton
Assessment Of Local Ecological Knowledge, Oyster Predation, And Habitat Function To Improve Management Of Nearshore Habitats, Cynthia Evelyn Marie Lupton
Theses and Dissertations
Coastal ecosystems along the northern Gulf of Mexico are ecologically and economically vital yet increasingly threatened by environmental change, habitat degradation, and knowledge gaps that complicate restoration and management efforts. This dissertation integrates social and ecological approaches to improve understanding of predator dynamics, stakeholder perceptions, and habitat function related to nearshore habitats. Thereby, providing information that can inform adaptive management and restoration in the region through addressing three overarching objectives: 1) evaluate stakeholder perceptions of oyster (Crassostrea virginica) predation and management practices; 2) quantify environmental and habitat factors influencing oyster drill (Stramonita haemastoma complex) abundance; and, 3) assess the influence …
Reproductive Genetics Of Caenorhabditis Nematodes, Dharani U. Matharage
Reproductive Genetics Of Caenorhabditis Nematodes, Dharani U. Matharage
Theses and Dissertations
Variation in reproductive traits and genome evolution reflects the interaction between selection, environment, and life-history. In the genus Caenorhabditis, recombination rate and sperm size exhibit a striking variation between strains and species. Here, Iintegrate three projects investigating recombination rate variation and recombination plasticity in C. elegans and sperm size divergence across Caenorhabditis species. First, I quantify recombination rates in two genetically distinct strains, N2 and CB4856, with different levels of laboratory adaptation using fluorescent markers spanning across gene-rich and gene-poor regions of the chromosome IV. I identify significant differences in recombination rate between the two strains within both gene-rich and …
Using Seafloor Geomorphology, Classification Schema, And Spatial Analysis To Predict Geologic Structures, With Applications For Mars, Emma Morgan
Theses and Dissertations
This study evaluated whether seafloor geomorphology can be used to predict subsurface fault locations along the Cascadia continental margin and explored whether such relationships can be transferred to Mars. Geomorphological classification schemas such as geomorphons, bathymetric position index (BPI), slope, rugosity, aspect, and curvature were derived from seafloor bathymetry data and used as predictor variables in maximum entropy (MaxEnt) models developed separately for Pre-Pleistocene, Pleistocene, Quaternary, and active fault datasets. Model performance ranged from AUC = 0.62 to 0.67, showing moderate predictive skill, with younger fault systems exhibiting stronger geomorphic associations. A secondary analysis showed a weaker but detectable relationship …
Unbounded Derivations On Algebras Associated With Monothetic Groups: An Expository Thesis, Britton Phillips
Unbounded Derivations On Algebras Associated With Monothetic Groups: An Expository Thesis, Britton Phillips
Theses and Dissertations
This thesis is an expository exploration of the paper Unbounded Derivations in Algebras Associated with Monothetic Groups. Monothetic groups will be utilized to create a minimal system that gives rise to two C*-algebras, and once we have these algebras, unbounded derivations will be able to be defined on them. These derivations are able to be classified and decomposed into ”special” derivations, and these decompositions help simplify the comparisons between derivations on different algebras. In particular, we emphasize the classification, covariance properties, innerness and approximate innerness, and the lifting behavior of these derivations.
Development And Evaluation Of A Closed-Loop Controlled Ugv With Rtk-Gps Navigation For Plastic Contamination Removal From Cotton Fields, Asifur Rahman
Development And Evaluation Of A Closed-Loop Controlled Ugv With Rtk-Gps Navigation For Plastic Contamination Removal From Cotton Fields, Asifur Rahman
Theses and Dissertations
Plastic contamination in cotton reduces fiber quality, causing an estimated $600 to $750 losses annually for the U.S. cotton industry. This work developed an RTK-GNSS-based UGV navigation for a collaborative UAV-UGV plastic retrieval system. An Amiga rover was adopted and modified, and a closed-loop PD steering control based on cross-track and heading error was developed and deployed in it. The first set of tests was conducted on a 111 m A-B line on a flat lane based on ASABE/ISO 12188-2 guidelines, then repeated in a cotton field. The P50 and P95, and max cross-track errors were about 1, 5, and …
Machine Learning Approaches For Predicting Biochemical Oxygen Demand And Ammonium Nitrogen: A Decade-Long Weekly Field Study At A Full-Scale Water Resource Recovery Facility, Hoda Khoshvaght, Ratish Ramyad Permala, Amir Razmjou, Mehdi Khiadani
Machine Learning Approaches For Predicting Biochemical Oxygen Demand And Ammonium Nitrogen: A Decade-Long Weekly Field Study At A Full-Scale Water Resource Recovery Facility, Hoda Khoshvaght, Ratish Ramyad Permala, Amir Razmjou, Mehdi Khiadani
Research outputs 2022 to 2026
Unlike previous studies that rely on high-frequency (15-min or hourly) datasets, this study is among the first to use low-frequency (weekly) data to evaluate the performance of linear and nonlinear machine learning (ML) algorithms for predicting biochemical oxygen demand (BOD) and ammonium nitrogen (NH4+-N) in the primary and secondary treatment effluents from the Subiaco Water Resource Recovery Facility (WRRF) in Western Australia. Various feature selection methods, including filters, wrappers, and embedded methods, were employed to identify the most effective approach that achieves the highest model performance while enhancing computational efficiency. The results demonstrate that a reduced set of …
Impact Of Travel Time To Health Facilities On Perinatal Outcomes: A Systematic Review With Narrative Synthesis And Meta-Analysis, Sanam Roder-Dewan, Marwa Ramadan, Paul Ouma, Alexander Manu, Emelda A. Okiro, Natalie Strobel, Bibian N. Robert, Emily Odipo, Peter M. Macharia, Anthony Danso-Appiah, David C. Goodman, Queen Dube, Gagan Gupta, Hema Magge, Karen Edmond
Impact Of Travel Time To Health Facilities On Perinatal Outcomes: A Systematic Review With Narrative Synthesis And Meta-Analysis, Sanam Roder-Dewan, Marwa Ramadan, Paul Ouma, Alexander Manu, Emelda A. Okiro, Natalie Strobel, Bibian N. Robert, Emily Odipo, Peter M. Macharia, Anthony Danso-Appiah, David C. Goodman, Queen Dube, Gagan Gupta, Hema Magge, Karen Edmond
Research outputs 2022 to 2026
Background: Evidence-based global guidance on safe travel time for small or sick newborns who require transfer to health facilities after birth is lacking. A two-hour threshold is frequently cited in low- and middle-income countries (LMICs), while 30 minutes is commonly used in high-income countries (HICs). Although these thresholds are widely referenced, their empirical basis and consistency across levels of newborn care and journey types have not been systematically examined. This study synthesises the evidence linking travel time and perinatal outcomes. Methods: We conducted a systematic review with narrative synthesis and meta-analysis to assess the impact of travel time or distance …
Review Of Sustainable Development In The Baked Food Industry : Innovations From Raw Materials, Processing To Packaging, Wang Yujia, Ma Shunzhang, Feng Muyu, Hou Dianzhi, Zhou Sumei
Review Of Sustainable Development In The Baked Food Industry : Innovations From Raw Materials, Processing To Packaging, Wang Yujia, Ma Shunzhang, Feng Muyu, Hou Dianzhi, Zhou Sumei
Food and Machinery
From the orientation of the global baked food industry toward health and sustainable development in recent years, this paper systematically reviews the innovations and progress across three core dimensions: raw material substitution, process optimization, and packaging improvement. First, regarding raw materials, conventional wheat flour is replaced with ingredients such as whole-grain flours and plant-based food by-products. Natural functional additives and novel protein sources (e.g., plant proteins, insect proteins, and microbial proteins) are developed to enhance the nutritional and health value of products and realize the efficient utilization of resources. Second, in the core process, techniques such as dough mixing, fermentation, …
Research Progress On Strain Identification And Wall-Breaking Technologies Of Wall-Broken Ganoderma Lucidum Spore Powder And Its Industrial Prospects, Han Lingxian, Liang Zengenni, Ji Hainan, Zhao Chan, Zhang Mengling
Research Progress On Strain Identification And Wall-Breaking Technologies Of Wall-Broken Ganoderma Lucidum Spore Powder And Its Industrial Prospects, Han Lingxian, Liang Zengenni, Ji Hainan, Zhao Chan, Zhang Mengling
Food and Machinery
Ganoderma lucidum spore powder consists of reproductive cells released during the mature stage of the fungus and concentrates the core nutrients and bioactive components of Ganoderma lucidum. Recent advances in the field of wall-broken Ganoderma lucidum spore powder were systematically summarized, with a focus on four core directions: the permitted scope and identification techniques of Ganoderma strains for health foods, optimization of wall-breaking processes and quality control, safety evaluation, and functions and mechanisms. Combined with policy orientation and market demand, future development suggestions were proposed, including strengthening strain management at the source, establishing a full-chain quality system, deepening mechanism research, …
Analysis Of Composition Variation In Ginseng With Different Fruit Colors Using Desorption Electrospray Ionization Mass Spectrometry Imaging Technology, Han Lusheng, Wang Yudan, Li Genyue, Yang Kaili, Wang Enpeng
Analysis Of Composition Variation In Ginseng With Different Fruit Colors Using Desorption Electrospray Ionization Mass Spectrometry Imaging Technology, Han Lusheng, Wang Yudan, Li Genyue, Yang Kaili, Wang Enpeng
Food and Machinery
[Objective] To investigate the differences between red-fruit and yellow-fruit ginseng from the perspective of compositional tissue distribution using mass spectrometry imaging technology. [Methods] Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) is employed to visually characterize saponin components in fresh ginseng tissues with two distinct fruit colors. Additionally, the total saponin, total flavonoid, and total polysaccharide content in the samples are quantitatively determined. [Results] Under the condition of mass spectral errors below 15 mg/kg, a total of 14 saponin components, including malonylated ginsenosides such as mRb 1, are identified. Significant spatial distribution differences in saponins are observed across tissues within the …
Purification, Enzymatic Properties, And Stabilizer Screening Of Acid Protease From Trichoderma Reesei, Wu Yao, Yu Huashun, Chen Rui, Yang Zhaoshi, Li Ming, Gao Jie, Zhang Yan
Purification, Enzymatic Properties, And Stabilizer Screening Of Acid Protease From Trichoderma Reesei, Wu Yao, Yu Huashun, Chen Rui, Yang Zhaoshi, Li Ming, Gao Jie, Zhang Yan
Food and Machinery
[Objective] To purify and study the enzymatic properties of acid protease from the Trichoderma reesei strain, and to screen the stabilizer formula. [Methods] The acid protease is purified by ammonium sulfate precipitation, dialysis, and anion exchange column chromatography. Then, its molecular weight is determined by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and its enzymatic properties are studied. Finally, the stabilizer formula of acid protease is optimized, with an investigation into its effect on acid protease storage stability. [Results] For the purified acid protease, its specific enzyme activity is 4 849 U/mg, purification fold is 3.28 times, and relative molecular weight is …