Biomedical Engineering and Bioengineering Commons^™

Targeting Glioblastoma: Mechanistic And Clinical Perspectives From Uofl Research, Swetha Senthil Nathan

The Cardinal Edge

Glioblastoma (GBM) remains one of the most aggressive and treatment-resistant brain tumors, with median survival lingering at 12–15 months despite surgery, radiation, and chemotherapy. This article explores cutting-edge research and clinical efforts at the University of Louisville aimed at improving GBM treatment outcomes. Dr. Joseph Chen investigates the tumor microenvironment, revealing how physical properties such as stiffness and porosity drive GBM cell proliferation, migration, and resistance to apoptosis. His lab’s findings suggest that porosity, rather than stiffness alone, may be a more accurate predictor of tumor invasion and progression-free survival. Additionally, his research highlights the role of hyaluronic acid and …

Ionic Liquid Pilocarpine Serves As Therapeutic Cosolvent And Permeation Enhancer For Glaucoma Medication, Ashish Trital, Lei Xu, Burhan Ates, Tzu Chen Wang, Vimalin Mani, Hu Yang

Chemical and Biochemical Engineering Faculty Research & Creative Works

The efficacy of hydrophobic ocular drugs is significantly hindered by their low bioavailability, for which poor water solubility is a major contributing factor. In this work, we synthesized pilocarpine-derived ionic liquid [Pilo-OEG] Cl (PO) and evaluated its cosolvent properties for the codelivery of the hydrophobic antiglaucoma drug brimonidine (BM) for glaucoma therapy. Pilocarpine was quaternized with 2-[2-(2-chloroethoxy) ethoxy] ethanol in a one-pot reaction to yield PO, and its structure was confirmed using ¹H NMR and FT-IR spectroscopy methods and further characterized for its rheological property and thermal stability. The HET-CAM assay and cell viability study showed that PO was …

Impact Of Glass Powder As A Sustainable Material On The Workability, Strength, And Durability Properties In High-Performance Basalt Fiber Reinforced Concrete: A Statistical Analysis, Ahmed Fathi Mohamed Salih

Journal of Sustainable Construction Materials and Technologies

The global construction sector is increasingly challenged to balance environmental sustainability with the growing demand for durable, high-performance materials. As cement production continues to be a major source of anthropogenic CO2 emissions, the incorporation of alternative, low-impact binders has become a key strategy for reducing the environmental footprint of concrete. In this context, glass powder (GP)—a finely ground industrial by-product rich in amorphous silica—has emerged as a promising partial replacement for Portland cement. Its pozzolanic reactivity contributes to improved hydration and matrix densification, while its use also supports circular economy principles by diverting waste glass from landfills. At the same …

Etv2 Mediated Differentiation Of Human Pluripotent Stem Cells Results In Functional Endothelial Cells For Engineering Advanced Vascularized Microphysiological Models, Shun Zhang, Zhengpeng Wan, Lei Wang, Caihong Wu, Junkai Zhang, Sarah Spitz, Xun Wang, Marie A. Floryan, Mark F. Coughlin, Francesca M. Pramotton, Liling Xu, Ron Weiss, Roger D. Kamm

Michigan Tech Publications

Patient-specific microphysiological models have become a valuable tool for broad applications, revolutionizing biomedical research. However, limitations persist, with functional vasculature being a significant challenge. With the discovery of ETV2's determinant role in specifying EC lineages during differentiation, researchers have adopted techniques involving ETV2 overexpression to produce h-iECs more efficiently and consistently. Here, we generated multiple h-iPSC lines with inducible ETV2 expression, and subsequently differentiated them into h-iECs, which were validated functionally and by key endothelial markers and RNA-seq analysis. These cells are capable of reproducibly self-organizing into stable microvascular networks (MVNs) in a microfluidic chip, forming lumenized and functional vessels …

Electrochemical Deactivation Of High-Strength, Catechol-Based Adhesives Incorporated With Anhydrous Proton And Electron Conducting Elements, Han Peng, Zhongtian Zhang, Vedika Khare, Abhilash Arjan Das, Fatemeh Razaviamri, Kan Wang, Bruce P Lee

Michigan Tech Publications

Catechol offers switchable adhesion in response to electrochemical redox reaction. However, electrochemistry requires water for effective proton transport, but water weakens adhesive performance. Here, we incorporate proton and electron conducting elements (sulfonic acid-containing monomer and multiwalled carbon nanotube, respectively) into a water-free catechol-based adhesive to create a high-strength adhesive that is also susceptible to electrochemical control. These additions increase the proton and electrical conductivity by over 100-fold. The adhesive also exhibits elevated lap shear adhesion strength (4.6 MPa) to metal substrates and outperforms a commercial epoxy glue. Under mild electrical stimulation (9 V), the adhesive strength decreases by over 90%. …

Translating Graduate Level Engineering Methods For K12 Students, Ahmad Bshennaty, Joey Marano, Hoda Hatoum

Michigan Tech Publications

Purpose

Advanced engineering tools such as particle image velocimetry (PIV) and computational fluid dynamics (CFD) are rarely introduced in K12 education despite their relevance in modeling engineering systems. This study evaluates whether K12 students can meaningfully engage with these traditional graduate-level methods when applied to cardiovascular flows and delivered through structured modules.

Methods

A 5-day full-time summer class was designed and enrolled 13 secondary students (grades 9–11) to introduce them to 3D printing, coding, PIV, medical image segmentation, and CFD modeling. Students participated in both experimental and computational activities and completed two evaluation forms assessing learning outcomes, instructional methods, challenges, …

Three-Dimensional Printing Of The Epineurium For Peripheral Nerve Repair: A Comprehensive Review Of Novel Scaffolds For Nerve Conduits, Alynah J. Adams, Iulianna C. Taritsa, Kaavian Shariati, Aaron Dadzie, Jose A. Foppiani, Maria Jose Escobar-Domingo, Daniela Lee, Angelica Hernandez-Alvarez, Kirsten Schuster, Helen Xun

School of Medicine Publications

Background: Nerve conduits are used to bridge peripheral nerve defects caused by trauma, iatrogenic injury, or oncologic disruption. Three-dimensional (3D) biomimetic scaffolds for peripheral nerve regeneration have advanced significantly in recent years, driven by improvements in printing technology and neuronal seeding techniques. We report on published designer conduits that can recreate the epineurium, a critical yet challenging-to-manufacture feature of nerve tissue.

Methods: A medical librarian conducted a literature search for our systematic review on EMBASE, Web of Science, and PUBMED, following PRISMA guidelines, for articles from January 2010 to January 2026 for the systematic review. Descriptive statistical analysis was performed …

Advancing Computational Analysis Of Fluorescence Imaging For Head And Neck Cancer Surgical Guidance, Yao Chen

Dartmouth College Ph.D Dissertations

The main goal of surgery for head and neck squamous cell carcinoma (HNSCC) is to remove the tumor completely while preserving as much healthy tissue as possible to enhance patient survival and reduce complications. However, achieving clear surgical margins is difficult due to the complex anatomy of the head and neck and the reliance on a surgeon’s visual and tactile assessments. Recently, near-infrared (NIR) fluorescence molecular imaging has shown potential for better tumor visualization during surgery, using targeted imaging agents like epidermal growth factor receptor (EGFR) probes. This has led to the development of fluorescence-guided surgery (FGS), which helps surgeons …

Fiber Optic Polarimetric Stress Sensor Improvements, Maxwell R. Richter, Max Randall, Mark C. Harrison

Engineering Faculty Articles and Research

The progression of diseases such as cancer has been correlated with tissue stiffness. For example, breast cancer tissue has been found to be stiffer than healthy mammary tissue. At present, stiffness measurement devices are not suitable for nondestructive, high-resolution measurements on small, delicate samples. We report a compact, portable, and sensitive fiber optic stress sensor that overcomes many of these limitations and is suitable for soft materials such as tissue. We demonstrate several improvements to a previous fiber stress sensor in physical hardware, software, and data analysis. We have designed and fabricated a custom enclosure to shield the sensitive setup …

Sensing Negative-Cone Rotational Diffusion Of Dipole-Like Emitters, Yuanxin Qiu, Kaizhi A. Nie, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Fluorescence anisotropy and single-molecule orientation-localization microscopy (SMOLM) are powerful techniques that quantify the rotational diffusion of dipole-like emitters, which is important for sensing molecular interactions and chemical environments at the nanoscale. Numerous theoretical and experimental studies have thoroughly characterized single-molecule rotations even when those rotations are much faster than the detector integration time. Here, we extend the theory of measuring rotational diffusion to situations where a single dipole rotates uniformly everywhere outside of an isotropic cone of a certain size, termed a negative cone. This scenario corresponds to negative fluorescence anisotropy 𝑟 and has been observed in emitters exhibiting strong …

Pid Control For Lower Limb Exoskeletons: A Framework Evaluation, Javlonbek Rakhmatillaev, Vytautas Bučinskas

Chemical Technology, Control and Management

This research provides a detailed guideline for implementing and evaluating Proportional Integral Derivative (PID) control frameworks in lower limb rehabilitation exoskeleton robotics. It examines the role of control systems within rehabilitation robotics, outlines the principles of PID control, describes exoskeleton architecture, explores applications of PID control, reviews optimization strategies, presents experimental validations, and considers future developments in the field. The proposed control framework incorporates aspects of mechanical design, actuator and sensor selection, and PID-based control algorithms, thereby promoting safe, accurate, and individualized rehabilitation support. Recommendations and effective guidance for future work are also presented.

Investigating Confounding Factors In Shear Wave Speed Measurements Of Fibrotic Liver Tissues: A Computer Simulation Study, Emily J. Miller, Yongmei Jin, Jingfeng Jiang

Michigan Tech Publications

Screening patients with liver fibrosis and identifying those at risk of developing advanced liver fibrosis is of clinical interest. Shear wave elastography (SWE), a promising non-invasive screening tool used to distinguish healthy tissue from diseased tissue, measures tissue shear wave speed (SWS) to describe tissue stiffness and identify liver fibrosis. However, considerable variations in the reported results have been found. We propose that the heterogeneity of the liver tissue background, such as the presence of fatty liver tissue and the preferred local orientation of the scarred fibrotic liver tissues embedded into the liver parenchyma, may contribute to the uncertainty in …

The Impact Of Human Myoblasts On Macrophage Polarization In A Coculture Model, Elisabeth Ann Stodola

Biomedical Engineering

Peripheral Artery Disease (PAD) is characterized by the buildup of atherosclerotic plaque in the lower extremities that occludes blood flow, and affects over 200 million people worldwide. Without effective treatment, PAD can lead to claudication, ischemia, ulcers, and gangrene. PAD causes more amputations in the United States than any other condition, and for severe cases that require revascularization, procedures benefit fewer than 50% of patients. This demonstrates a clear need for alternative therapies. Potential therapies for PAD target expansion of collateral blood vessels around the area of occlusion – a process called arteriogenesis to compensate for loss of blood flow …

Design And Validation Of A Wearable Respiratory Protection System For Wildland Firefighters, Oliver Pilon

Master's Theses

Wildland and Wildland–Urban Interface firefighters are routinely exposed to hazardous smoke with fine particulate matter, carbon monoxide, volatile organic compounds, and other toxic combustion products. Existing protective gear has significant limitations and shortcomings for these conditions. Respirators with tight-fitting facepieces make it difficult to breathe and speak. On the other hand, self-contained breathing apparatuses are too heavy, have a limited duration of use, and do not work well with long wildland fire scenes, while smaller protective equipment like N-95’s and gaiters provide very little protection. These constraints contribute to low adoption rates and prolonged occupational exposure.

This thesis presents the …

Plm-Effector: Unleashing The Potential Of Protein Language Models For Bacterial Secreted Protein Prediction, Dandan Zheng, Lihong Chen, Guansong Pang, Jian Yang

Research Collection School Of Computing and Information Systems

Bacterial secreted proteins, particularly effectors delivered by specialized secretion systems, are key mediators of virulence and host-pathogen interactions. However, accurate computational identification remains challenging, as many existing methods rely heavily on sequence similarity or handcrafted features, and often focus on a single secretion system. Recent studies have reported that some bacterial effectors may be associated with more than one secretion system, highlighting the complexity of secretion system annotation and motivating the development of system-aware computational prediction approaches. Here, we present PLM-Effector, a hybrid deep learning framework that integrates modern protein language models (PLMs) with multiple neural architectures via a two-layer …

Development Of An Automated Platform For Cell Biology Experiments, Dipendra Nepal

Shelby Hall Graduate Research Forum Posters

Cell biology experiments involve repetitive tasks such as pipetting, sample preparation, and imaging, which are typically performed manually. These manual processes can introduce variability, reduce efficiency, and increase the cost of biomedical research. To address these challenges, our lab is developing a modular robotic platform, iEye™, to automate key steps in cell biology workflows [1]. The system is designed as self-contained modules, allowing flexible development and integration of different functionalities. In this work, we focus on a sample preparation module that integrates a 6-degree-of-freedom robotic arm, a motorized stage, and a pneumatic pipetter. This module is being developed to automate …

Ai Driven Assessment For Clinical Scenarios, Sudhanshu Tarale, Bhushan Lohar, Ninad Pandit

Shelby Hall Graduate Research Forum Posters

Beta 1 captured granular data to validate the system’s ability to measure clinical thoroughness and deliver real-time feedback. Data collection formally approved by the USA IRB (Protocol 25 037; #22849172).

Tourniquet Tester, Tyler Offerman, Thuy Nguyen, Ryan Ly, Cody Hoeptner

Biomedical Engineering

The Tourniquet Tester is a device intended to be used as a standardized testing device for tourniquets. Due to the lack of a standard testing device for testing tourniquets that can produce accurate and reproducible results, it is difficult to test the efficacy of tourniquets currently being sold on the market. A standard testing device that can produce consistent and reproducible results would allow tourniquet manufacturing companies to properly test the efficacy of their product and develop proper guidelines and directions for users of the product. Medical professionals and military personnel who may use tourniquets in their line of work …

Babycheck, Ayla V. Campbell, Eleanor M. Tweedy

Biomedical Engineering

The BabyCheck project was created to design and test a device that can collect a small blood sample from the fetal scalp during labor. The goal is to reduce uncertainty when fetal heart rate tracings are unclear, especially in Category II cases where it can be difficult for clinicians to decide the safest next step. Current monitoring methods either rely on interpreting heart rate patterns or use more invasive procedures to gather biochemical information. BabyCheck was designed to provide a simpler and more controlled way to collect a small blood sample that could help guide these decisions.

The final prototype …

Jaundease Phototherapy Vest Test Plan, Sujay Panthulu, Jasdev Banwait, Gwynn Moss, Solomon M. Fernandez

Biomedical Engineering

Neonatal hyperbilirubinemia is a common condition affecting more than 60% of newborns, caused by an excess buildup of bilirubin in the blood due to the liver’s limited ability to process it efficiently [1]. The standard treatment involves hospital-based phototherapy, where infants are placed in incubators under blue light to break down bilirubin in the skin. Although effective, this approach requires infants to remain under the lights for 12–18 hours per day, often with eye protection, and separated from their parents—disrupting early bonding and potentially contributing to toxic stress that can impact neurological and emotional development [2]. While at-home phototherapy solutions …