Biomedical Engineering and Bioengineering Commons^™

Bio-Payload Senior Design Report, Connor Bishop, Oliver Goodall, Marcus Kirk, Gabby Robles, Anish Shanmuganathan

Interdisciplinary Design Senior Theses

This project focuses on the design and development of an autonomous experimental platform capable of conducting cellular biology experiments in a well plate within a CubeSat environment. The system integrates microfluidics, robotics, and onboard sensors to remotely initiate experiments, monitor them, and collect data without human intervention. The objective is to create a platform for automated biological experimentation in microgravity, while reducing reliance on ground-based control and increasing mission efficiency and reproducibility. The team used Saccharomyces cerevisiae to assess the biocompatibility of the well plate and monitor changes in cell culture, including optical density and cell viability.

Evaluation Of An Affordable Prosthetic Liner In Resource Limited Settings, Samerial Brown

Posters - 2026

The lower limb prosthesis is an important assistive technology for persons with limb amputations and differences. The interface (prosthetic liner) between the rigid prosthetic socket and the residual limb is often made of silicone, urethane or thermoplastic elastomer gel. This material provides cushioning and support as well as suspension for the transtibial (below knee) prosthetic leg. Unfortunately the cost of liners range from $3-$600. This cost precludes its use in resource limited settings such as Sri Lanka. As a result patients are fit with a foam liner which has less cushioning and suspension capabilities than a gel type liner. This …

Electromyography, Prosthetic Properties, And Running Biomechanics In Recreationally Active Persons With Lower Extremity Amputation, Paige M. Agnew

Rehabilitation Sciences Theses & Dissertations

Lower extremity loss affects nearly 150,000 individuals in the United States yearly yielding extreme health care costs annually. Typically, prosthetic devices are prescribed for 5-8 years despite known changes to an individual’s gait as time progresses. Patients can expect to undergo approximately 8-12 weeks of gait retraining to improve their comfort and efficiency. Biomechanical changes occur when running with a specific running prosthetic including considerable biomechanical and neuromuscular interlimb asymmetries and alterations in loading patterns. The purpose of this dissertation was to investigate the biomechanical demands for lower extremity prosthesis users during running. The first study synthesized peer reviewed evidence …

Fabrication Of Flexible Laser-Induced Graphene-Based Non-Enzymatic Glucose Sensor For Diabetes Management, Reagan Aviha

Electrical & Computer Engineering Theses & Dissertations

This research focuses on developing a flexible non-enzymatic glucose sensor to address the issues of cost, stability, and limited sensitivity associated with enzymatic glucose sensors. Regular monitoring of glucose levels is required to manage the global health challenge of diabetes. The sensor is built on a cost-effective, durable, flexible, conductive, and high-surface-area substrate acting as a scaffold for modification with easily tunable and electroactive species to enhance glucose detection. The electroactive materials used are zinc oxide (ZnO), palladium (Pd) and platinum (Pt). Laser-induced graphene (LIG) is created by laser engraving. ZnO, Pd and Pt are electrochemically deposited and optimized based …

Mechanisms Of Synergy Between Nanosecond Pulsed Electric Field (Nspef) And Cold Atmospheric Pressure Plasma Discharge (Cap) In Pancreatic Cancer: The Role Of Ros-Mediated Oxidative Stress And Apoptosis, Zobia Minhas

Theses and Dissertations in Biomedical Sciences

Pancreatic ductal adenocarcinoma is a highly lethal malignancy characterized by poor prognosis and resistance to conventional therapies. Novel treatment strategies are needed to overcome this resistance. Non-thermal physical modalities, including nanosecond pulsed electric field (nsPEF) and cold atmospheric pressure plasma discharge (CAP), have emerged as potential anticancer approaches; however, the mechanisms underlying their combined effects remain unclear.

This study evaluated whether nsPEF enhances CAP-induced cytotoxicity and investigated the associated molecular mechanisms using the murine Pan02 pancreatic cancer cell line. Cell viability was assessed by WST-1 metabolic activity assays and electric cell–substrate impedance sensing (ECIS). Intracellular reactive oxygen species (ROS) were …

Evaluating Human Rod Photoreceptor Function Using Pixelwise Intensity-Based Optoretinography, Gao Yang, Mina Gaffney, Robert F. Cooper

Biomedical Engineering Faculty Research and Publications

Purpose: More than 50 inherited retinal diseases are known, with rod photoreceptor functional loss serving as an early indicator in nearly half of them. The “optoretinogram” is a relatively new assay that detects optical changes in cells in response to stimuli. This tool has excellent potential for providing insights into the earliest functional changes of rod photoreceptors, with the potential to assist in the early detection, monitoring, and treatment of retinal diseases.

Methods: In this work, we obtained intensity-based optoretinograms (iORGs) from rod photoreceptors using an adaptive optics scanning laser ophthalmoscope. We explore the necessity of both individual rod identification …

Severity And Duration-Dependent Aortic Stiffening In A Rabbit Coarctation Model Identifies Constitutive Material Parameters As Promising Regional Biomarkers For Hypertension Progression, Arash Ghorbannia, Jamasp Azarnoosh, John F. Ladisa Jr.

Biomedical Engineering Faculty Research and Publications

Coarctation of the aorta (CoA) alters hemodynamics and drives regional remodeling, yet the influence of severity and duration on constitutive properties is undefined. We quantified mechanical responses from CoA to identify constitutive parameters capturing stiffening across clinical severities and durations. Mild (≤ 12 mmHg), intermediate (13–20 mmHg), and severe (> 20 mmHg) CoA was created in rabbits for short, long, and prolonged durations (~ 1, 3, or 22 weeks, respectively). Stress–stretch curves from proximal and distal regions were fit with multiple constitutive models (Linear Elastic, Neo-Hookean, Yeoh, Ogden, Mooney–Rivlin, Holzapfel). Model performance was evaluated by normalized RMSE and R², with …

Development And Acceptability Of A Patient Decision Aid For People With Degenerative Cervical Myelopathy: An International Mixed-Methods Study, Andrew R. Gamble, David B. Anderson, Marnee J. Mckay, Benjamin M. Davies, Sophie Macpherson, James Van Gelder, Tammy Hoffmann, Kirsten Mccaffery, Samuel X. Stevens, Carlo Ammendolia, Rohil V. Chauhan, Carl M. Zipser, Timothy F. Boerger, Lindsay A. Tetreault, Michael G. Fehlings, Elanor Dustan, Carolyn Nugent, Helen Holmgren, Andreas K. Demetriades, Justin M. Lantz, Rana Dhillon, Chris G. Maher, Joshua R. Zadro

Biomedical Engineering Faculty Research and Publications

Objectives To develop and user-test a patient decision aid for people diagnosed with degenerative cervical myelopathy and who are considering surgery.

Design Mixed-methods study describing the development of a patient decision aid.

Setting A draft decision aid was developed by a multidisciplinary steering group (including study authors with degenerative cervical myelopathy, health professionals and researchers) informed by the best available evidence, authorship consensus and existing patient decision aids.

Participants Patient-participants and health professional-participants who manage people with degenerative cervical myelopathy were recruited through social media and the steering group’s research and practice network. Quantitative questionnaires were used to gather baseline …

Bioengineering The Future Of The Human Species: H+’S Ideological Iterations Of Trans-/Posthumanity As Exemplified By Futurist Literary Texts = (Bio)Technological Solutions For (And/Or) Reification Of Systemic Inequities?, Marjorie E. Bomhower

English Theses & Dissertations

While humanity has, throughout our species’ history, endeavored to dream and create astonishing, and ever-evolving, (bio)technologies with the utopian ideals of bettering our day-to day lives and achieving greater longevity, our endeavors have (thus far) not only produced remarkable, beneficial advances in longevity and quality of life, but have also yielded devastating and dire outcomes; often at the cost and victimization of marginalized communities. This dissertation focuses on select (bio)technologies – particularly, those promoted by H+ (the most current and popular technoutopianist non-profit, “educational,” organization) – with an investigation of current and nascent (bio)technological evolutions, futurist literary texts, and critical/digital …

Plant-Mediated Green Synthesis Of Zinc Ferrite Nanoparticles: Mechanisms, Properties, And Advanced Applications In Environmental And Biomedical Fields, Rihab Jabbar

Al-Bahir

Recently, plant-mediated green synthesis has emerged as a sustainable, eco-friendly, and cost-effective strategy for synthesizing zinc ferrite (ZnFe₂O₄) nanoparticles (NPs). This review critically examines the advancements in biosynthesizing ZnFe₂O₄ NPs using various plant extracts, including Aloe vera, Hibiscus rosa-sinensis, Aegle marmelos, Eucalyptus leaves, Tragacanth gum, Piper nigrum, and Moringa oleifera, among others. It emphasizes their dual role as reducing, capping, chelating, and stabilizing agents. The article extensively compares physicochemical properties of the green-synthesized NPs in terms of crystallite size, morphology, magnetic behavior, and band gap energy. Achieved through different synthesis routes such as sol-gel, hydrothermal, co-precipitation, and microwave-assisted methods. …

Recombinant Gelonin: Purification And Functional Validation For Electroporation-Assisted Delivery, Eleni Zivla, Giedrė Šilkūnienė, Andrei G. Pakhomov, Mantas Šilkūnas, Olga N. Pakhomova

Knowledge and Creativity Expo

Type I ribosome-inactivating proteins, such as gelonin, are enzymatically potent toxins but are effectively non-toxic under physiological conditions because they are unable to cross the plasma membrane[1]. Pulsed electric fields (PEF) overcome this delivery barrier by transiently permeabilizing the plasma membrane, allowing direct cytosolic entry. This enhances gelonin cytotoxicity by several thousand-fold, highlighting gelonin’s potential as an electrochemotherapy candidate[2]. Here, we present a recombinant gelonin (rGel) expression platform in E. coli with HPLC-based purification that enables reproducible protein production and structure-guided engineering.

The mature gelonin sequence (residues 47–297) was cloned into pET-32a(+) with an N-terminally fused Trx-6×His tag and TEV …

Application Of Microbial Fuel Cells For Leachate Bioremediation: The Effects Of Nutrient Nourishment, Umi Sholikah, Fidela Chosta, Tegar Palyus Fiqar, Eka Masrifatus Anifah, Riza Hudayarizka, Tadashi Hibino

Makara Journal of Science

Leachate contains a high organic content, which can cause environmental pollution. The organic content in leachate can be removed through bioremediation using microbial fuel cell (MFC) systems to remediate pollutants and generate bioelectricity. Organic matter is important to the MFC process as both a substrate and a nutrient source. This study investigated the effects of glucose and sodium phosphate buffer nourishment on BOD, COD, and TSS removal and bioelectricity generation. This study used leachate as a substrate and a graphite rod with a surface area of about 32.98 cm² as an electrode. The reactor uses plastic blocks with a …

Recent Advances In Graphene-Based Field-Effect Transistor Biosensors For Disease Biomarker Detection And Clinical Prospects, Deeksha Nagpal, Anup Singh, John Link, Abijeet Mehta, Ashok Kumar, Vinay Bhudhraja

Health & Biomedical Sciences Faculty Publications

Field-effect transistor (FET) biosensors using graphene have become one of the most promising biosensing platforms for the early diagnosis of diseases with features such as high sensitivity, label-free detection and application compatibility with point-of-care systems. Herein, we critically discuss recent advances in graphene FET (GFET) biosensor development toward clinically relevant biomarkers associated with representative diseases including cancer, neurodegenerative disease, infectious disease, and inflammatory conditions. Recent progress was reviewed to evaluate GFET architectures, surface functionalization methods, and detection quality. The biomarkers explored were clusterin in Alzheimer’s disease, thrombin in coagulopathy, estrogen receptor α (ER-α) in breast cancer, Carcinoembryonic antigen in lung …

Mechanical, Thermal And Fire Behaviour Of Hemp-Based Composites With Different Binders For Sustainable Building Applications, Anilcan Aygun, Fahri Birinci, Selim Aytac, Ali Kemal Ayan, Hasan Alp Sahin, Ibrahim Isildak

Journal of Sustainable Construction Materials and Technologies

This study investigates the physical, mechanical and thermal properties of hemp-based composites produced with traditional binders such as lime, cement, gypsum, clay, and water-based glue. Hemp fractions of different sizes (splinter, shiv, fiber and powder) were combined with these binders to manufacture cube and plate specimens, which were tested for physical, mechanical, thermal, and temperature resistance properties. The study aims to compare the mechanical, physical, thermal, and fire behaviour of these composites to assess their suitability for sustainable construction applications. The hemp-based composites were tested for unit weight, water absorption, compressive strength and thermal conductivity. Most specimens are lightweight (<1000 kg/m3), and exhibit good thermal insulation performance and high compressive deformability, making them suitable for seismic-prone regions. The glue-based composite had the lowest unit weight, measured as 330 kg/m3. Thermal conductivity ranged from 0.051 to 0.243 W/m· K, with several mixtures performing better than natural pumice. While cement-based composites showed the highest compressive strength, ranging from 5.5 to 24.8 kg/cm2, natural hydraulic lime is the lowest. Water absorption was high, and moisture release occurred slowly across all hemp fractions. Combustion tests indicated that hemp-based materials have moderate temperature resistance, with charring temperatures ranging from 238–265°C for raw hemp and 300–385°C when mixed with binders. Overall, hemp-based composites are lightweight, eco-friendly, and suitable for non-load-bearing construction and insulation applications. However, improvements in moisture resistance and long-term durability are necessary to enhance their practical applicability.

Sustainable Cow Dung Bricks: An Innovative Approach To Eco-Friendly Masonry With Enhanced Thermal And Mechanical Performance, Nagesh Sugandhi, Sachin Patil, Satish Reddy, Ashwini G, Santosh Kumar

Journal of Sustainable Construction Materials and Technologies

This study explores the development, characterization, and performance of sustainable cow dung bricks using locally sourced organic (cow dung) and mineral (clay, quartz sand, feldspar, lime) constituents for non-load-bearing masonry in India. Three blends with varying cow dung content (70%, 75%, 80%) were mixed, molded, air dried, and cured without kiln firing to reduce environmental impact. The bricks' mechanical properties—compressive strength, water absorption, bulk density, flexural strength, shore hardness, and modulus of elasticity—were systematically evaluated with complete statistical documentation (n = 6 replicates per formulation per test age). Microstructural features were investigated using Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray …

Sustainable Cement-Blast Furnace Slag Mortar, Richa Jain, Harshit Jain, Ashish Nim

Journal of Sustainable Construction Materials and Technologies

New building materials that required and produced less energy are need of today's infrastructure development. The ability of such building materials to promote sustainable development always puts its demand in active mode. With huge consumption of naturally occurring materials in building construction, the problem related to environment degradation is now common for world. Along with this, world is also facing the problem of reuse, recover and scientifically approved disposal of non-biodegradable wastes. Blast furnace slag (BFS) is one of those steel industry wastes that are abundantly available and their disposal arises as prime challenge for industries. This research work makes …

Polymer Microstructures For Advanced Biomanufacturing, Tongyao Wu

LSU Doctoral Dissertations

With the continued growth of the biopharmaceutical industry, the demand for scalable, robust, and resource-efficient platforms for large-scale mammalian cell culture is amplified. Recent developments in microfluidic technology, such as precise control of the microenvironment, showed the potential to improve the performance of cell culture systems. However, constrained by scalability and operational efficiency, applying such approaches to large-scale cell culture and biopharmaceutical production presents challenges. This dissertation addresses these challenges through three independent but conceptually related technological developments. First, a roll-to-roll (R2R) fabrication process was developed for the scalable production of hollow microcarriers (HMCs). HMCs provide three-dimensional microenvironments suitable for …

Bicuspid Valve Closure And Backflow Prevention: Role Of Leaflet Geometry, Badr Kauoi, A Bou Orm, P Navet, James W. Baish, Lance Munn

Faculty Journal Articles

Bicuspid valves with crescent-shaped leaflets are found in lymphatic vessels and veins, where their primary function is to prevent reflux and ensure unidirectional flow toward the heart. These valves are passive, and their functionality emerges spontaneously from a complex interplay between the properties of the valve leaflets and the flow patterns developing within the vessel sinus region surrounding the valve. The main function of the valves is to limit retrograde flow, or reflux, but the optimal valve structure has not been well characterized. Here we investigate numerically how the length of the leaflets affects the valve efficiency in preventing reflux. …

Development And Preliminary Pressure Control Validation Of Woundwatch: Npwt System With Remote Monitoring Capabilities, Swetha Senthil Nathan

The Cardinal Edge

Chronic wounds, including diabetic foot ulcers, pressure sores, and venous leg ulcers, affect over 40 million individuals worldwide and are often complicated by delayed healing due to limited access to continuous monitoring. Negative Pressure Wound Therapy (NPWT) is an established treatment that promotes wound healing through controlled sub-atmospheric pressure. However, conventional NPWT systems lack real-time physiological feedback and require frequent in-person follow-ups, limiting their effectiveness in remote or low-resource settings. This study presents WoundWatch, a portable, low-cost NPWT device that integrates real-time temperature, humidity, and pressure sensing with wireless data transmission via an IoT platform. The system includes a vacuum …

The Developing Role Of Ai In Modern Engineering Research, Rianna Pais

The Cardinal Edge

No abstract provided.