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In Vitro Biomechanical Comparison Of Clamped Suture Knot Techniques As A Model Of Extra-Capsular Stabilization Of Canine Cruciate Ligament Repair, Jung-Jin Lee, Mu-Young Kim, Hun-Young Yoon 2021 Chulalongkorn University

In Vitro Biomechanical Comparison Of Clamped Suture Knot Techniques As A Model Of Extra-Capsular Stabilization Of Canine Cruciate Ligament Repair, Jung-Jin Lee, Mu-Young Kim, Hun-Young Yoon

The Thai Journal of Veterinary Medicine

Six clamped suture knot techniques were compared to identify the effects on the biomechanic properties used as a model of extra-capsular stabilization. Six clamped suture knot techniques included square knot formed by clamping the first throw with toothed mosquito forceps (SQ-TM), non-toothed mosquito forceps (SQ-NTM), or needle holder (SQ-NH) and surgeon's knot formed by clamping the first throw with toothed mosquito forceps (SG-TM), non-toothed mosquito forceps (SG-NTM), or needle holder (SG-NH). Monotonic loading test was performed on each suture-loop. Initial loop tension, ultimate load, load at 3 mm elongation, elongation at failure, stiffness, and failure mode were compared among techniques. …


Association Of Clinical Parameters And A Formula For Predicting The Total Antioxidant Power In Cats, Kanissarinn Sakundech, Worapol Aengwanich 2021 Chulalongkorn University

Association Of Clinical Parameters And A Formula For Predicting The Total Antioxidant Power In Cats, Kanissarinn Sakundech, Worapol Aengwanich

The Thai Journal of Veterinary Medicine

The objective of this study was to investigate external appearances, vital signs, hematological parameters, malondialdehyde and total antioxidant power to find correlations and to predict total antioxidant power in cats. Body condition scores, mucous membrane color scores, heart rate, respiratory rate, body temperature, hematological parameters, malondialdehyde and ferric reducing antioxidant capacity of 27 cats were measured. Correlation and multiple regression models were analyzed. The results revealed the following information: respiratory rate vs. heart rate, packed cell volume vs. hemoglobin, total red blood cell vs. packed cell volume, total red blood cell vs. hemoglobin, mean corpuscular hemoglobin vs. mean corpuscular volume, …


Evaluation Of Poly (Ethylene Glycol–Propylene Glycol) Copolymer Bone Hemostatic Agent Containing Gentamicin For Enhancing Local Antibacterial And Anti-Inflammatory Effects In An Infected Calvarial Defect Rat Model, Jeon-Mo Kim, Hun-Young Yoon 2021 Chulalongkorn University

Evaluation Of Poly (Ethylene Glycol–Propylene Glycol) Copolymer Bone Hemostatic Agent Containing Gentamicin For Enhancing Local Antibacterial And Anti-Inflammatory Effects In An Infected Calvarial Defect Rat Model, Jeon-Mo Kim, Hun-Young Yoon

The Thai Journal of Veterinary Medicine

Bone wax is the most commonly used hemostatic agent in surgery; however, it is non-absorbable and may act as a nidus of infection. Thus, new hemostatic agents that are absorbable and have antibiotic effects are needed. In this study, we assessed the hemostatic ability, bioabsorption, and local antibiotic and anti-inflammatory effects of a newly developed poly(ethylene glycol–propylene glycol) (PEG-PPG) copolymer containing gentamicin (PEG-PPGg) in a calvarial defect rat model. A total of 100 Sprague Dawley rats were used in this study. Twenty were used to determine time to hemostasis and bioabsorption of PEG-PPGg. The remaining rats were assigned to four …


Immunostimulatory Effect Of Disinfectant On Bees, Tatiana Romanishina, Svitlana Guralska, Tetiana Kot, Svitlana Furman, Oleh Pinsky, Diana Feshchenko, Zhanna Rybachuk, Olexiy Tkachenko, Olga Zastulka, Svitlana Tushak 2021 Chulalongkorn University

Immunostimulatory Effect Of Disinfectant On Bees, Tatiana Romanishina, Svitlana Guralska, Tetiana Kot, Svitlana Furman, Oleh Pinsky, Diana Feshchenko, Zhanna Rybachuk, Olexiy Tkachenko, Olga Zastulka, Svitlana Tushak

The Thai Journal of Veterinary Medicine

The article presents a modern interpretation of the immunostimulatory effect of the disinfectant “Biocontact Plus” on the bee organism. The minimum threshold concentrations of the disinfectant with bactericidal effect were determined in vitro. The stimulating effect of 0.15 % of the concentration of active substances of the disinfectant has been confirmed in vivo. The immunostimulatory effect of this concentration on the immune status of bee colonies is interpreted (the number of young and mature immunocytes). The immunomodulatory effect of 0.15 % disinfectant “Biocontact Plus” on bee haemolymph allows for the use of alternative therapies for bacterial diseases of bees in …


The Efficiency Of Herbal On Blood Clotting And Wound Healing Of Ear Notched Pigs, Kittiphong Tippaya, Ruttayaporn Ngasaman, Kridda Chukiatsiri 2021 Chulalongkorn University

The Efficiency Of Herbal On Blood Clotting And Wound Healing Of Ear Notched Pigs, Kittiphong Tippaya, Ruttayaporn Ngasaman, Kridda Chukiatsiri

The Thai Journal of Veterinary Medicine

This study aimed to determine the efficiency of Jatropha podagrica Hook. f., Chromolaena odorata (L.) and Centella asiatica (L.) on the reduction of blood clotting and promoting wound healing in notched piglets. Thirty-four notched piglets aged 2–3 days were selected for applying fresh exudes of J. podagrica Hook. f. to the right ear and povidone iodine to the left ear as a control. In the experiment of C. odorata (L.) and C. asiatica (L.), fifty notched piglets were divided into five groups and 10, 20, 30, 40 and 50% each of herbal gel was applied to the right ear and …


Uplc-Qtof/Ms-Based Metabolomic Analysis Of Plasma Reveals Altitude Effects On Yaks (Bos Grunniens), Xiangqiong Meng, Juan Zhou, Juan Zhou, Ruidong Wan, Hongxian Yu, Qing Wei 2021 Chulalongkorn University

Uplc-Qtof/Ms-Based Metabolomic Analysis Of Plasma Reveals Altitude Effects On Yaks (Bos Grunniens), Xiangqiong Meng, Juan Zhou, Juan Zhou, Ruidong Wan, Hongxian Yu, Qing Wei

The Thai Journal of Veterinary Medicine

The yak is an ideal animal model for the study of adaptation to hypoxia at high altitudes. Its blood metabolites are an important reference index for research regarding adaptation to high-altitude hypoxia and can reflect cellular activity, antioxidant capacity and the metabolic capacity of yaks, thus highlighting the adaptability of the yak to hypoxic environments. The aim of our study was to evaluate differences in the global metabolic profiles of plasma in high- and low-altitude yaks (average age: 3 years old; live weight (LW): 300 ± 50 kg) using untargeted metabolomics. 52 differential metabolites were identified; 13 metabolites such as …


Comparison Of The Postoperative Outcome Of The Three-Port Laparoscopic Ovariectomy And Conventional Open Ovariectomy Methods In Dogs, Damla Tuğçe Okur, Bülent Polat 2021 Chulalongkorn University

Comparison Of The Postoperative Outcome Of The Three-Port Laparoscopic Ovariectomy And Conventional Open Ovariectomy Methods In Dogs, Damla Tuğçe Okur, Bülent Polat

The Thai Journal of Veterinary Medicine

The aim of this study is to determine the effects of laparoscopic ovariectomy (LOVE) and conventional open ovariectomy (COVE) methods on early-stage surgical stress parameters [proinflammatory cytokines (TNF-α and IL-6), acute phase proteins (CRP, Hp), inducible nitric oxide synthase (INOS), mitogen activated protein kinase (MAPK14) and cortisol], pain scores and D-Dimer (DD) levels in 36 healthy bitches. Three-port laparoscopic ovariectomy was performed on the LOVE group while routine ovariectomy was performed on the COVE group. Surgical stress parameters and pain scores (University of Melbourne pain scale) were assessed during the pre- (0 hour) and postoperative periods (1st, 3rd, 6th hour …


The First Report Of Antimicrobial Residues In Cultured Tilapia Fillets In Three Provinces Of Lao Pdr, Seangphed Phommachanh, Bundit Tengjaroenkul, Peerapol Sukon, Lamyai Neeratanaphan, Kanit Chukanhom 2021 Chulalongkorn University

The First Report Of Antimicrobial Residues In Cultured Tilapia Fillets In Three Provinces Of Lao Pdr, Seangphed Phommachanh, Bundit Tengjaroenkul, Peerapol Sukon, Lamyai Neeratanaphan, Kanit Chukanhom

The Thai Journal of Veterinary Medicine

The objective of this study was to investigate antimicrobial residues in the fillets of Nile tilapia fish (n=180) from 3 provinces (Vientiane, Savannakhet Province and Champasak Province) in Lao PDR in the summer, rainy and winter seasons. Clean meat test (CMT) was used for screening the antimicrobial residue samples. Then, the antimicrobial residue groups were examined and identified by the european six-plate test (ESPT), and the concentration of each positive antimicrobial residue from the ESPT was further assessed by high-performance liquid chromatography (HPLC). The evaluation results of the three antimicrobial detection methods were 19.44% (35/180) for the CMT, 22.86% (8/35) …


Zinc Supplementation Improves Semen Quality In Boars, Supitcha Kaewma, Sanya Suphappornchai, Junpen Suwimonteerabutr, Nutthee Am-In, Mongkol Techakumphu 2021 Chulalongkorn University

Zinc Supplementation Improves Semen Quality In Boars, Supitcha Kaewma, Sanya Suphappornchai, Junpen Suwimonteerabutr, Nutthee Am-In, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

One of the causes of infertility in boars is nutritional deficiency. Interestingly, zinc has been used for improving semen quality in boars. Thus, the objective of this study was to investigate the effect of zinc as feed supplementation in order to improve semen quality in infertile boars. Three purebred Pietrain boars with a poor semen quality history were selected for the experiment. The semen samples were collected once a week for 4 weeks before the treatment period and the semen evaluated immediately. Fifteen grams (a total concentration of 150 ppm) of zinc were supplemented daily in the normal feed. The …


Dynamics And Evolution Of Genotype 1 Porcine Reproductive And Respiratory Syndrome Virus Following Its Introduction Into A Herd Concurrently Infected With Genotypes 1 And 2, Thitima Tripipat, Kepalee Saeng-chuto, Adthakorn Madapong, Christopher James Stott, Angkana Tantituvanont, Pavita Kaewprommal, Jittima Piriyapongsa, Dachrit Nilubol 2021 Chulalongkorn University

Dynamics And Evolution Of Genotype 1 Porcine Reproductive And Respiratory Syndrome Virus Following Its Introduction Into A Herd Concurrently Infected With Genotypes 1 And 2, Thitima Tripipat, Kepalee Saeng-Chuto, Adthakorn Madapong, Christopher James Stott, Angkana Tantituvanont, Pavita Kaewprommal, Jittima Piriyapongsa, Dachrit Nilubol

The Thai Journal of Veterinary Medicine

This study was conducted to investigate the dynamics and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) genotype 1 following its introduction into a herd concurrently infected with both PRRSV genotypes. The results demonstrated that 2 novel genotype 1 groups emerged, termed clusters II and III. The emergence of cluster II suggested that this cluster of genotype 1 virus potentially correlated with the outbreak. Then, cluster III was detected with the disappearance of cluster II. The evolution of the genotype 1 virus from cluster II to cluster III is likely to be associated with immune evasion induced by amino …


The Feasibility And Image Quality Of Using Soft Embalming Cadaver Dogs And Cats For Radiographic And Ultrasonographic Training, Chutimon Thanaboonnipat, Nan Choisunirachon, Chalika Wangdee, Srireepong Kiertkrittikhoon, Voraphan Na Songkhla, Sirakarnt Dhitavat, Chanyaratt Chaichareonchon, Lampetch Vimuktalop, Kiatpichet Komin 2021 Chulalongkorn University

The Feasibility And Image Quality Of Using Soft Embalming Cadaver Dogs And Cats For Radiographic And Ultrasonographic Training, Chutimon Thanaboonnipat, Nan Choisunirachon, Chalika Wangdee, Srireepong Kiertkrittikhoon, Voraphan Na Songkhla, Sirakarnt Dhitavat, Chanyaratt Chaichareonchon, Lampetch Vimuktalop, Kiatpichet Komin

The Thai Journal of Veterinary Medicine

Diagnostic imaging techniques, especially radiography and ultrasonography, have been applied as routine diagnostic tools in clinical practice of both human and veterinary medicine. Soft embalmed cadavers by Thiel’s method is a new method for preserving dead bodies that has been increasingly used in medical education especially in diagnostic imaging fields. The aims of this study were, first, to examine the feasibility of using canine and feline soft cadavers in radiographic and ultrasonographic study and training. Second, to evaluate the procedure score and image quality score of radiography and ultrasonography using the soft cadaver dogs and cats as the model. All …


Diagnostic Value Of C-Reactive Protein And Soluble Urokinase Plasminogen Activator Receptor In Canine Monocytic Ehrlichiosis, Songul Erdogan, Onur Basbug, Ugur Aydogdu, Serdar Pasa, Hasan Erdogan, Kerem Ural 2021 Chulalongkorn University

Diagnostic Value Of C-Reactive Protein And Soluble Urokinase Plasminogen Activator Receptor In Canine Monocytic Ehrlichiosis, Songul Erdogan, Onur Basbug, Ugur Aydogdu, Serdar Pasa, Hasan Erdogan, Kerem Ural

The Thai Journal of Veterinary Medicine

Canine monocytic ehrlichiosis is a vector-borne disease that can induce renal failure in dogs. In this study we aimed to describe the potential diagnostic efficiency of CRP and suPAR in dogs infected with E. canis and the association between suPAR and CRP with renal failure. For this purpose, the study consisted of a total of 17 dogs, 8 of infected with E. canis and 9 of healty. For evaluating renal failure, plasma urea-creatinine and UPC were determined and also measurements of plasma CRP and suPAR were analysed with ELISA test kits. Mean CRP, suPAR, urea, creatinine and UPC levels of …


Schirmer Tear Test 1 As A Method To Measure Tear Production Value In Normal Kintamani Dogs, Steven Dwi Purbantoro, Erika Kadek Yudha Ariawan, Endah Rahmawati, Zefanya Christiani, Sri Kayati Widyastuti 2021 Chulalongkorn University

Schirmer Tear Test 1 As A Method To Measure Tear Production Value In Normal Kintamani Dogs, Steven Dwi Purbantoro, Erika Kadek Yudha Ariawan, Endah Rahmawati, Zefanya Christiani, Sri Kayati Widyastuti

The Thai Journal of Veterinary Medicine

Keratoconjunctivitis sicca (KCS) is a dry eye condition due to an inadequacy of the eyes to produce tears. This condition can be diagnosed using the Schirmer Tear Test 1 (STT1). The Kintamani dog, a Chinese Chow Chow-derivate, is now popular globally to be kept by dog lovers. This study aims to determine the normal value of tear production in Kintamani dogs. Forty eyes from above one-year-old twenty Kintamani dogs were used in this study. Tear production value was measured using STT1 strip. The result showed that the mean of tear production in Kintamani dogs was 23.37±4.415 mm/min. Statistically, there was …


Systemic Aspergillosis Involving The Mediastinum Associated With Antifungal Therapy In A Dog, Nawat Sannamwong, Saikaew Sutayatram, Nardtiwa Chaivoravitsakul, Patharakrit Teewasutrakul, Sawang Kesdangsakonwut, Chollada Buranakarl 2021 Chulalongkorn University

Systemic Aspergillosis Involving The Mediastinum Associated With Antifungal Therapy In A Dog, Nawat Sannamwong, Saikaew Sutayatram, Nardtiwa Chaivoravitsakul, Patharakrit Teewasutrakul, Sawang Kesdangsakonwut, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

A four-year old, intact female Labrador Retriever dog was presented with a history and clinical signs of vomiting, anorexia and mild dyspnea. Further laboratory examination, by specific canine pancreatic lipase (cPL) test kit and abdominal ultrasound, indicated acute pancreatitis. Results from thoracic radiography and computerized tomography scan showed masses in the cranial and middle mediastinal areas. A cytology examination revealed pyogranulomatous inflammation with the presence of fungal hyphae whereas Aspergillus niger was isolated and identified. Systemic aspergillosis was diagnosed. Many antifungal drugs were introduced and the clinical outcomes were discussed with careful consideration.


Carp Edema Virus A Rising Threat To Global Carp Population, Xolani Phaya, Jing-Xiang Zhou, Zhuo Yu Wang, Hao Wang, Jie Liang 2021 Chulalongkorn University

Carp Edema Virus A Rising Threat To Global Carp Population, Xolani Phaya, Jing-Xiang Zhou, Zhuo Yu Wang, Hao Wang, Jie Liang

The Thai Journal of Veterinary Medicine

Carp edema virus (CEV), a poxvirus from the family Poxviridae, was first detected in 1974 in Japan and has since spread to most parts of the world. CEV is the etiological agent of koi sleepy disease (KSD), which has adverse health effects on both koi and common carp fish species. Equally, KSD leads to detrimental financial distress to carp farmers and further threatens both food security and ecology. The onset and severity of CEV/KSD is influenced by water temperature and handling stress. The prominent clinical sign of KSD in both koi and common carp is lethargy, which is manifested when …


Reversible Pulmonary Hypertension Secondary To Bronchopneumonia In A Kitten, Jidapa Tosuwan, Sirilak Disatian Surachetpong 2021 Chulalongkorn University

Reversible Pulmonary Hypertension Secondary To Bronchopneumonia In A Kitten, Jidapa Tosuwan, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

A five-month-old Siamese kitten was presented with acute respiratory distress. The kitten had respiratory acidosis. The result of the feline N-terminal prohormone of brain natriuretic peptide (NT-proBNP) test kit was abnormal. Thoracic radiography revealed patchy lung infiltration and cardiomegaly. Severe right heart enlargement and an intermediate probability of pulmonary hypertension were suggested from echocardiography. Pulmonary hypertension secondary to severe pneumonia was suspected. The kitten was treated with oxygen supplementation, antibiotics and pimobendan. The clinical signs, radiography and echocardiography returned to normal after 30 days of treatment.


Major Bacterial Pathogens Of Bovine Respiratory Disease And Lung Lesions In Calves From Selected Areas Of Ethiopia, Mirtneh Akalu, Behadra Murthy, Takele Abayeneh, Esayas Gelaye 2021 Chulalongkorn University

Major Bacterial Pathogens Of Bovine Respiratory Disease And Lung Lesions In Calves From Selected Areas Of Ethiopia, Mirtneh Akalu, Behadra Murthy, Takele Abayeneh, Esayas Gelaye

The Thai Journal of Veterinary Medicine

Bovine respiratory disease (BRD) is a potential threat and an economically detrimental disease of calf-rearing in Ethiopia. This study was designed to isolate the major bacterial pathogens associated with respiratory disease of calves and the lung lesions involved. A crosssectional study with a purposive sampling method was employed in 170 calves during the study period from April 2018 to October 2018. The Bacteriological and molecular assay revealed overall isolation of 156 (91.76%) bacterial pathogens. Gross inspection of pneumonic lungs revealed one or more lesions of hydatidosis (46.67%), emphysema (44.44%), congestion (35.56%), hemorrhage (20.0%) and atelectasis (15.56%). Bacterial pathogen identification showed …


Expression Profile Analysis Of Micrornas During The Oestrous Cycle Of Qira Black Sheep, Xi Chen, Hanying Chen, Song Jiang, Chaocheng Li, Xiancun Zeng 2021 Chulalongkorn University

Expression Profile Analysis Of Micrornas During The Oestrous Cycle Of Qira Black Sheep, Xi Chen, Hanying Chen, Song Jiang, Chaocheng Li, Xiancun Zeng

The Thai Journal of Veterinary Medicine

Ovarian follicular development is closely regulated by a multitude of genes and specific non-coding RNAs (ncRNAs), which are typically altered during the oestrous cycle. Identification of the microRNAs (miRNA) expression profiles in sheep ovaries during the oestrous cycle is a prerequisite to further understanding their functional status. This study presents a genome-wide analysis of miRNAs from three groups of Qira black sheep: oestrus (day 0), dioestrus (day 10) and pro-oestrus (day 14). A total of 545 conserved miRNAs and 206 novel miRNAs were identified. Furthermore, 229 differentially expressed miRNAs were screened in three comparisons and 16 miRNAs were found to …


Pharmacokinetics Of Tulathromycin In Pregnant Ewes (Ovis Aries) Challenged With Campylobacter Jejuni, Michael Yaeger, Jonathan P. Mochel, Zuowei Wu, Paul Plummer, Orhan Sahin, Joseph Smith, Melda Ocal, Ashenafi Beyi, Changyun Xu, Qijing Zhang, Ronald W. Griffith 2021 Iowa State University

Pharmacokinetics Of Tulathromycin In Pregnant Ewes (Ovis Aries) Challenged With Campylobacter Jejuni, Michael Yaeger, Jonathan P. Mochel, Zuowei Wu, Paul Plummer, Orhan Sahin, Joseph Smith, Melda Ocal, Ashenafi Beyi, Changyun Xu, Qijing Zhang, Ronald W. Griffith

Faculty Publications and Other Works -- Large Animal Clinical Sciences

The purpose of this study was to evaluate the pharmacokinetics of tulathromycin in the plasma and maternal and fetal tissues of pregnant ewes when administered within 24 hours of a single, IV Campylobacter jejuni (C. jejuni) challenge. Twelve, pregnant ewes between 72–92 days of gestation were challenged IV with C. jejuni IA3902 and then treated with 1.1 ml/45.36 kg of tulathromycin subcutaneously 18 hours post-challenge. Ewes were bled at predetermined time points and euthanized either at a predetermined time point or following the observation of vaginal bleeding or abortion. Following euthanasia, tissues were collected for bacterial …


Equine Influenza Virus And Vaccines, Fatai S. Oladunni, Saheed Oluwasina Oseni, Luis Martinez-Sobrido, Thomas M. Chambers 2021 Texas Biomedical Research Institute

Equine Influenza Virus And Vaccines, Fatai S. Oladunni, Saheed Oluwasina Oseni, Luis Martinez-Sobrido, Thomas M. Chambers

Veterinary Science Faculty Publications

Equine influenza virus (EIV) is a constantly evolving viral pathogen that is responsible for yearly outbreaks of respiratory disease in horses termed equine influenza (EI). There is currently no evidence of circulation of the original H7N7 strain of EIV worldwide; however, the EIV H3N8 strain, which was first isolated in the early 1960s, remains a major threat to most of the world’s horse populations. It can also infect dogs. The ability of EIV to constantly accumulate mutations in its antibody-binding sites enables it to evade host protective immunity, making it a successful viral pathogen. Clinical and virological protection against EIV …


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