Oral Administration Of Iron And Toltrazuril In Combination Improves Survival Rate And Growth Rate And Reduces Anaemia In Piglets,
2022
Chulalongkorn University
Oral Administration Of Iron And Toltrazuril In Combination Improves Survival Rate And Growth Rate And Reduces Anaemia In Piglets, Pachara Pearodwong, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study was performed to determine the efficacy of an oral administration of iron and toltrazuril in combination on the growth rate, survival rate and the incidence of anaemia in suckling piglets. A total of 406 piglets were randomly allocated to one of three groups: i.) CONTROL (n = 161), the piglets were administered iron dextran 200 mg intramuscularly and toltrazuril (20 mg/kg) orally at 3 days of age; ii.) NEGATIVE (n = 84), the piglets were not treated with iron until 7 days of age, and toltrazuril was given at 5 days of age and iii.) TREATMENT (n …
Generation Of Tail Bearing Sperm-Like Cells From In Vitro Spermatogenesis Of Farming Goat Testis,
2022
Chulalongkorn University
Generation Of Tail Bearing Sperm-Like Cells From In Vitro Spermatogenesis Of Farming Goat Testis, Rakesh Bhaskar, Bijaylaxmi Sahoo, Sung Soo Han, Mukesh Kumar Gupta
The Thai Journal of Veterinary Medicine
In vitro spermatogenesis offers a therapeutic modality for the treatment of male infertility associated with pre-or post-meiotic barriers to the spermatogenesis process. The present study reports the production of tail-bearing sperm-like cells from in vitro spermatogenesis of farming goat testis in an air-liquid inter-phase culture. The testicular fragments (1-2 mm3) were placed on agarose scaffolds at air-liquid interphase and cultured in vitro for 60 days. Proliferating germ cell colonies were visible after 45-55 days of culture. Differentiation of cells to spermatogonia and spermatocytes was observed from the 40th day onwards. Tail bearing sperm-like elongated cells, with apical acrosome, were detected …
Odor Detection Of Cancer Cell Metabolites By Scent-Detection Dogs,
2022
Chulalongkorn University
Odor Detection Of Cancer Cell Metabolites By Scent-Detection Dogs, Geontae Park, Dongwook Kim, Seongsoo Jeong, Dongsun Park, Gonhyung Kim
The Thai Journal of Veterinary Medicine
Dogs have a superior olfactory system; thus, they have been trained and used to detect various nonbiological and biological scents. In addition, many studies have recently reported dogs’ ability to detect the odor of certain cancers or cancer cells. A previous study documented that a dog trained to detect a certain malignant cancer cell could also detect another, unfamiliar malignant cancer cell well, implying that these two cancer cells share a certain specific odor. Thus, given the hypothesis that malignant cancer cells of different origins may contain a common cancer-specific odor, the purpose of the present study was to evaluate …
Computed Tomographic Findings Of Primary Renal Tumors In Dogs And Cats,
2022
Chulalongkorn University
Computed Tomographic Findings Of Primary Renal Tumors In Dogs And Cats, Daji Noh, Jaejin Shim, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee
The Thai Journal of Veterinary Medicine
Primary renal tumor is uncommon in dogs and cats, with few previous computed tomography (CT) reports. Previous studies have reported that contrast-enhancing CT is helpful in differentiating the type of renal tumors. This study aimed to describe CT findings of renal tumors in dogs and cats and identify contrast-enhancing pattern according to 3 post-contrast phases. In this retrospective study, the following CT findings were recorded for each patient: (1) renal tumor involvement, (2) enhancement pattern, (3) vascular invasion, (4) presence of lymphadenopathy and organ metastasis, (5) presence of mineralization, and (6) attenuation values of renal tumors on pre- and post-contrast …
Ovarian Stimulation With A Low Dose Of Follicle Stimulating Hormone Improved Recovery Rates And Developmental Competence Of Goat Oocytes,
2022
Chulalongkorn University
Ovarian Stimulation With A Low Dose Of Follicle Stimulating Hormone Improved Recovery Rates And Developmental Competence Of Goat Oocytes, Pintira Thiangthientham, Nitira Anakkul, Junpen Suwimonteerabutr, Wirakan Kallayanathum, Sawita Santiviparat, Mongkol Techakumphu, Theerawat Tharasanit
The Thai Journal of Veterinary Medicine
The aim of this study was to optimise the ovarian responses using different FSH (follicle stimulating hormone) treatments in goats. Group 1 (control), the ovaries were stimulated with a total dose of 200 mg FSH per doe. The FSH injections were performed at 12-h intervals starting on day 9 after progesterone implantation. The FSH doses were decreased to 160 mg/doe and 130 mg/doe for Groups 2 and 3, respectively. The numbers of small, medium and large follicles were determined by real-time B mode ultrasonography. Ovum pick-up (OPU) was performed by laparoscopy under general anesthesia at 96, 42 and 36 h …
Comparison Of Biomechanical Properties Associated With The Diameter And Insertion Depth Of Bone Anchors In The Femoral Condyle Of Toy Breed Cadaver Dogs,
2022
Chulalongkorn University
Comparison Of Biomechanical Properties Associated With The Diameter And Insertion Depth Of Bone Anchors In The Femoral Condyle Of Toy Breed Cadaver Dogs, Yun-Hee An, Mu-Young Kim, Hun-Young Yoon
The Thai Journal of Veterinary Medicine
The objective of the present study was to compare the biomechanical properties of implanted bone anchors based on diameter and insertion depth to yield selection criteria for bone anchor size in toy breed dogs. Twenty toy breed dog cadavers weighing < 5 kg underwent placement of five types of veterinary bone anchors (short diameter, short length [SDSL]; short diameter, medium length [SDML]; medium diameter, medium length [MDML]; medium diameter, long length [MDLL]; and long diameter, medium length [LDML]) at a predetermined femoral attachment site. Anchor screw depth/femoral condyle width (FCW) and anchor screw diameter/femoral condyle length (FCL) were measured using radiography. The yield load, Young’s modulus and failure load were measured and the causes of failure for each construct were recorded. The anchor screw depth/FCW was < 50%, 50%–75%, ~50%, and 75%–100% in the SDSL, SDML, MDML, and MDLL groups, respectively. The yield load, Young’s modulus and failure load were higher in the SDML and MDLL groups than in the SDSL and MDML groups. The anchor screw diameter/FCL was 12%–15% and 24%–30% in the SDML and LDML groups, respectively. No differences in biomechanical parameters were found between the SDML and LDML groups. The cause of failure in all constructs was pull-out of the bone anchor, except for distal femur fracture in five LDML constructs. In conclusion, when implanting bone anchors in toy breed dogs, the insertion depth should be > 50% of the FCW, regardless of diameter. Moreover, distal femur fracture can occur if the bone anchor diameter/FCL ratio exceeds 24%.
Suspected Malignant Mesothelial Cells In Bronchoalveolar Lavage Fluid Of A Dog With Respiratory Distress,
2022
Chulalongkorn University
Suspected Malignant Mesothelial Cells In Bronchoalveolar Lavage Fluid Of A Dog With Respiratory Distress, Junseok Kim, Jimin Oh, Taesik Yun, Yoonhoi Koo, Dohee Lee, Yeon Chae, Byeong-Teck Kang, Mhan-Pyo Yang, Hakhyun Kim
The Thai Journal of Veterinary Medicine
An 11-year-old neutered female Labrador Retriever presented with a 1-week history of respiratory distress, lethargy and anorexia. On physical examination, the dog exhibited dyspnea with a rapid and shallow breathing pattern. Thoracic radiography showed diffuse nodular or miliary interstitial patterns, which raised suspicion of bacterial, fungal and parasitic infections and metastatic tumors. Bronchoalveolar lavage (BAL) was performed and the culture yielded no growth of infectious agents, including bacteria and fungi. A presumptive diagnosis of pulmonary mesothelioma was made based on malignant mesothelial cells on BAL fluid cytology. Therefore, this is a report identifying suspected pulmonary mesothelioma using BAL fluid in …
Application Of A Single-Tank Sequencing Batch Reactor For Long-Term Zebrafish Care,
2022
Chulalongkorn University
Application Of A Single-Tank Sequencing Batch Reactor For Long-Term Zebrafish Care, Aranya Ponpornpisit, Malinee Jongjaroenjai, Naowarat Suthamnatpong, Surachet Burut-Archanai
The Thai Journal of Veterinary Medicine
Sequencing batch reactors are efficient wastewater treatment systems, however, they are not adapted to treat aquaria. We aimed to compare the efficacies of a modified single-tank sequencing batch reactor (SSBR) and a regular overflow filtration system. Zebrafish were maintained in an acrylic aquarium connected to an SSBR or in a tank connected to a regular overflow filtration system for 27 weeks. Water change or aeration using air stone was not performed. The water quality in both groups was within the normal range until 26 weeks, after which the nitrite and nitrate levels increased. The average temperature was, 29.46 ± 0.71°C …
A Systematic Review: The Source Of Antimicrobial Resistance Pathogenic Bacteria On Slaughtered Meat, Cross-Contamination Or Self-Contamination?,
2022
Chulalongkorn University
A Systematic Review: The Source Of Antimicrobial Resistance Pathogenic Bacteria On Slaughtered Meat, Cross-Contamination Or Self-Contamination?, Xin Wu, Fanan Suksawat, Sunpetch Angkititrakul, Chunlian Song, Jun Chai, Yifang Zhang, Xianghua Shu, Huiyan Xing, Wengui Li
The Thai Journal of Veterinary Medicine
Slaughtered meat has been identified as one of the major vehicles for forming antimicrobial resistance (AMR). It is still believed that AMR pathogens introduced by overusing antimicrobials in the breeding industry are directly brought in from farm animals. This disregards however the fact that many gut pathogens do not settle on muscle parts; the presence of gut pathogens on muscle products does not directly correlate to farm feeding. To investigate whether the source of AMR bacteria on slaughtered meat was cross-contamination or self-contamination, many peer-reviewed publications were studied to assess if the observational studies focused on AMR pathogenic bacteria association …
Semen Production Capacity Among Boar Breeds And Effect Of Organic Antioxidant Supplementation On Sperm Characteristics During The Hot Season In Thailand,
2022
Chulalongkorn University
Semen Production Capacity Among Boar Breeds And Effect Of Organic Antioxidant Supplementation On Sperm Characteristics During The Hot Season In Thailand, Congbang Ngo, Suriya Sooksong, Nutthee Am-In, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study was carried out to investigate semen production capacity among boar breeds and measure the efficiency of a new organic antioxidant supplementation on semen production and qualities during the hot season in Thailand. A total of 711 ejaculates from 87 boars (i.e., 26 Duroc, 15 Landrace, 30 Yorkshire and 16 crossbred Landrace × Yorkshire) were included in the study. Semen samples were allocated into two treatments – without organic antioxidant (CONTROL; n = 397) and with organic antioxidant supplementation (TREATMENT; n = 314). The experiment was carried out for three months during the hot season in May (n …
Haplotype Variation Of Partial Sry Gene In Ongole Grade Bulls (Bos Indicus) Of Indonesia,
2022
Chulalongkorn University
Haplotype Variation Of Partial Sry Gene In Ongole Grade Bulls (Bos Indicus) Of Indonesia, Widya Pintaka Bayu Putra, Saiful Anwar, Slamet Diah Volkandari, Syahruddin Said
The Thai Journal of Veterinary Medicine
Ongole grade cattle (Bos indicus) are Indonesian beef cattle that adapt well in a tropical climate. This study was aimed to observe the genetic diversity of partial Sex-determining of the region Y chromosome (SRY) gene (625 bp) in Ongole grade bulls (Bos indicus) of Indonesia. A total twenty-three (23) DNA samples of animal studies were collected from frozen sperm (straws) produced by the National Artificial Insemination Centers (NAICs) of Lembang (13 straws) and Singosari (10 straws). Research showed that a total of four (4) insertion mutations were detected in the intronic region with the position of 1831th, 1844th, 1850th and …
A Study Of Standard Values Of Two-Dimensional, M-Mode And Doppler Echocardiography In Conscious Small Domestic Rabbits,
2022
Chulalongkorn University
A Study Of Standard Values Of Two-Dimensional, M-Mode And Doppler Echocardiography In Conscious Small Domestic Rabbits, Nattanicha Ngosurachet, Tipparat Tangtraitha, Preeyanit Kaewyara, Pattharat Asvaesoontorn, Thawat Lekdumrongsak, Sirilak Disatian Surachetpong
The Thai Journal of Veterinary Medicine
To determine normal cardiac values for two-dimensional, M-mode and Doppler echocardiography because standard echocardiographic values have not been adequately studied in small-breed rabbits. Data was collected from 40 healthy small-breed rabbits. The results of complete physical examination, thoracic radiography, electrocardiography and echocardiography were collected and analysed as descriptive statistics. The correlation between weight and age and echocardiographic parameters was evaluated. A t-test was performed to compare the parameters between males and females. Most parameters were normally distributed and could be reliably used as standard values of echocardiographic parameters. Weight and age correlated positively with several echocardiographic parameters. However, there was …
Environmental Enrichment And The Behavioral Effects On Captive Beluga Whales (Delphinapterus Leucas),
2022
Chulalongkorn University
Environmental Enrichment And The Behavioral Effects On Captive Beluga Whales (Delphinapterus Leucas), Tsung-Hsien Li, Evonne Lim, I-Chun Chen, Chuen-Fu Lin, Shih-Chu Chen, Ming-An Tsai
The Thai Journal of Veterinary Medicine
Environmental enrichment methods are meant to encourage animals to display their complete ethogram while under human care in zoos and aquariums. In this study, we sought to characterize the effectiveness of environmental enrichment (involving the inclusion of toys in the living environment) on captive beluga whales in the National Museum of Marine Biology and Aquarium. Our results indicate that belugas with toys spend less time floating and swimming repetitively than do belugas without toys. The presence of toys was also shown to enhance social interactions among captive belugas. Overall, our findings indicate that the provision of toys can enrich the …
Molecular Determination Of West Nile Virus, Equine Herpesvirus 1 And Herpesvirus 4 In Feral Horses In Turkey,
2022
Chulalongkorn University
Molecular Determination Of West Nile Virus, Equine Herpesvirus 1 And Herpesvirus 4 In Feral Horses In Turkey, Irmak Dik, Oguzhan Avci, Sibel Yavru, H. Pelin Aslim, H.Sercan Palanci
The Thai Journal of Veterinary Medicine
West Nile Virus (WNV), Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) are among the important pathogenic viral factors for both domestic and feral horses. This study was carried out to determine the molecular presence of WNV, EHV-1 and EHV-4 infections in feral horses in Konya. Blood samples were taken from 36 horses selected by a randomized sampling method from a total of 250 feral horses bred together. The leukocyte samples obtained were examined virologically for the presence nucleic acid of WNV using the Nested reverse transcriptase-polymerase chain reaction assay (nested RT-PCR) method and using molecular methods for the …
Pueraria Mirifica Can Modulate The Ovarian Activity Of Crossbred-Thai Native Does,
2022
Chulalongkorn University
Pueraria Mirifica Can Modulate The Ovarian Activity Of Crossbred-Thai Native Does, Phubet Satsook, Sukanya Rattanatabtimtong, Lak Piasai, Patcharapa Towiboon, Chalermchart Somgird, Anuchai Pinyopummin
The Thai Journal of Veterinary Medicine
To evaluate the effect of P. mirifica supplementation on ovarian activity in crossbred Thai native does, a study was carried out for three consecutive estrus cycle periods: 1) D0-D21(pre-treatment), 2) D21-D42 (treatment) and 3) D42-D63 (post-treatment); D0, D21 and D42 were the days of expected estrus. P. mirifica was orally fed at 1000 mg/kg BW/d for 21 days in supplemented groups. Twenty-one does were equally divided into three groups: 1) CON – control, no supplementation and 2) supplemented groups included 2.1) FEC – start feeding at early-cycle (D21-D42), and 2.2) FMC – start feeding at mid-cycle (D32-D53). Estrus behavior was …
Sex-Associated Differences In Patellotrochlear Morphology In Small-Breed Dogs,
2022
Chulalongkorn University
Sex-Associated Differences In Patellotrochlear Morphology In Small-Breed Dogs, Radka S. Garnoeva
The Thai Journal of Veterinary Medicine
The purpose of this study was to make a between-sex comparison of trochlear groove and patellar metrics in healthy dogs on tangential radiographs. A total of 38 stifle joints from 19 healthy dogs (9 males; 10 females) from three small breeds (Pomeranian, Pinscher, Chihuahua) were evaluated. The following morphometric parameters of patella and trochlear groove were measured: trochlear sulcus angle (SA), lateral and medial trochlear inclination angles (LTI; MTI), trochlear groove depth (TD), retropatellar angle of Wiberg (WA), lateral and medial patellar facet angles (LFA, MFA), lateral and medial facet lengths (LF, MF), horizontal and vertical patellar diameters. In the …
Microbial Diversity And Microbiological Quality Of Edible-Birdnest (Ebn) During Processing And Storage,
2022
Chulalongkorn University
Microbial Diversity And Microbiological Quality Of Edible-Birdnest (Ebn) During Processing And Storage, Shazreena Zuber, Zunita Zakaria, Jalila Abu, Saleha Abdul Aziz
The Thai Journal of Veterinary Medicine
The microbial quality of 45 EBN consisting of raw unclean and raw clean Edible-birdnest (EBN) from two different premises were analyzed for 8 weeks. Using 3M™ Petrifilm, Total Aerobic Count (TAC) of raw clean EBN and Total Coliform Count (TCC) for raw unclean, 3 days, 2 weeks and 4 weeks EBN in both premises exceeded the limit stated in by the Malaysian Standard (MS 2334:2011; SIRIM/DVS 3:2015). A total of 264 bacteria and 208 fungi species were isolated. Bacteria isolated was identified using bioMérieux’s API® kit and a series of biochemical tests. Fungi were identified using techniques described by the …
Cloning, Expression And Identification Of Truncated Spike Gene Of Ibv (Sf200) And Chicken Granulocyte- Macrophage Colony Stimulating Factor (Gm-Csf),
2022
Chulalongkorn University
Cloning, Expression And Identification Of Truncated Spike Gene Of Ibv (Sf200) And Chicken Granulocyte- Macrophage Colony Stimulating Factor (Gm-Csf), Tran Duc Hoan, Doan Thi Thao, Wang Bin
The Thai Journal of Veterinary Medicine
The aim of this study was to clone, express and identify the truncated S1 gene of nephrotropic infectious bronchitis virus (IBV) and granulocyte-monocyte colony stimulating factor (GM-CSF) of chicken. Firstly, two genes were amplified by polymerase chain reaction (PCR) and cloned into pMD18-T vector. The truncated S1 gene designated as Sf200 (containing five antigenic sites of S1 glycoprotein on amino acid residues (aa) 24–61, (aa) 291–398 and (aa) 497–543 and GM-CSF were then amplified from the respective recombinant pMD18-T plasmids and cloned into pET-32a (+) vector resulting pET-Sf200, pET-GM which were identified by restriction enzyme digestion and sequencing analysis. The …
Efficacy Of Immune Complex Infectious Bursal Disease Vaccines Against Very Virulent Infectious Bursal Disease Virus Challenge In Commercial Layer Chicken,
2022
Chulalongkorn University
Efficacy Of Immune Complex Infectious Bursal Disease Vaccines Against Very Virulent Infectious Bursal Disease Virus Challenge In Commercial Layer Chicken, Nataya Charoenvisal, Phornphisut Areeraksakul, Choew Kong Mah, Nida Sirikobkul, Nida Sirikobkul
The Thai Journal of Veterinary Medicine
The vaccination program for controlling Infectious bursal disease (IBD) was complicated due to high maternal antibody in day old chick. Therefore, our study was performed in young layer chickens with high maternal antibody as commonly seen in our country. The objective of this study was to determine the protective efficacy of immune complex IBD vaccines against very virulent infectious bursal disease virus (vvIBDV) challenge in commercial layer chickens. As immune complex vaccine A was recommended in layers by the company, the first aim of the study was to compare immune complex vaccine A (group 1) and B (group 3). The …
Acquired Indirect Unilateral Chronic Reducible Scrotal Hernia In An Arabian Stallion,
2022
Chulalongkorn University
Acquired Indirect Unilateral Chronic Reducible Scrotal Hernia In An Arabian Stallion, Mohamed A. Marzok, Adel I. Almubarak, Mahmoud Kandeel, Adel A. Badawy
The Thai Journal of Veterinary Medicine
This case report describes the presentation and surgical management of acquired indirect unilateral chronic reducible scrotal herniation in an 8-year-old Arabian stallion. The horse had a history of moderate unilateral swelling of the scrotum that had been noticed by the owner for 10 months. Upon palpation, the swelling was soft, reducible, painless and not accompanied by an acute abdomen. Ultrasonography, rectal and clinical examination were useful tools for the diagnosis of scrotal hernias in this case. A successful surgical herniorrhaphy was performed with the removal of the testicle at the affected side and the superficial inguinal ring was closed by …
