Veterinary Medicine Commons^™

Exposure Of Egyptian Rousette Bats (Rousettus Aegyptiacus) And A Little Free-Tailed Bat (Chaerephon Pumilus) To Alphaviruses In Uganda, Rebekah C. Kading, Erin M. Borland, Eric C. Mossel, Teddy Nakayiki, Betty Nalikka, Jeremy P. Ledermann, Mary B. Crabtree, Nicholas A. Panella, Luke Nyakarahuka, Amy T. Gilbert, Julian C. Kerbis-Peterhans, Jonathan S. Towner, Brian R. Amman, Tara K. Sealy, Barry R. Miller, Julius J. Lutwama, Robert M. Kityo, Ann M. Powers

USDA Wildlife Services: Staff Publications

The reservoir for zoonotic o’nyong-nyong virus (ONNV) has remained unknown since this virus was first recognized in Uganda in 1959. Building on existing evidence for mosquito bloodfeeding on various frugivorous bat species in Uganda, and seroprevalence for arboviruses among bats in Uganda, we sought to assess if serum samples collected from bats in Uganda demonstrated evidence of exposure to ONNV or the closely related zoonotic chikungunya virus (CHIKV). In total, 652 serum samples collected from six bat species were tested by plaque reduction neutralization test (PRNT) for neutralizing antibodies against ONNV and CHIKV. Forty out of 303 (13.2%) Egyptian rousettes …

Equine Kinesiology Tape Application To Equine Hock Joints: Impact On Lameness And Movement Evaluation, Kiara Jones

Honors College Theses

Discomfort in the hocks of horses can cause lameness, resulting in poor performance. Diagnosis by veterinarians typically includes evaluating movement or use of ultrasound or radiography to evaluate tendons, ligaments, or bones. Treatment methods and times vary depending on diagnosis, but often include joint injections, medications, and stall rest. Treatments can be costly both financially and regarding time off from practice or competition. Alternative practices such as using equine kinesiology tape to support joints may improve comfort and movement for lower cost. The objective of this project was to evaluate the ease of use and effects of equine kinesiology tape …

Seroprevalence And Titer Concentration Testing For Leptospirosis In Equine, Ashley Himmelsbaugh

Honors College Theses

The most common best management practice used to combat diseases in horses is vaccination, which can decrease the incidence or severity of illness. However, the duration of immunity will vary for each vaccination and must be considered for revaccination purposes. Titer concentration, which evaluates antibodies in the blood, may be a helpful way of determining how long an animal has immunity to a disease. Leptospirosis, a zoonotic, bacterial disease, can result in uveitis, potentially leading to blindness, and abortion in mares. One serovar, Leptospirosis pomona (L. pomona), is associated with most cases of clinical disease in horses in North America. …

Predicting Immunization Status At Arrival In Tennessee Stocker Calves, Claire E. Hunkler

Masters Theses

Bovine respiratory disease is a multifaceted disease with agent, host, and environmental factors. Stocker calves are at high risk of contracting the disease through many stressors like weaning, transportation, commingling, etc. The purpose of this project was to test if externally observed physical characteristics of calves on arrival at a stocker facility can be used to predict calfhood vaccination status determined based on antibody titer levels. Knowledge of highly correlated characteristics could allow stocker operators to reduce the occurrence of BRD through targeted management strategies, thus lowering morbidity, mortality, and treatment costs. Ear notches, blood, and visual characteristics were collected …

Divergent Serpentoviruses In Free-Ranging Invasive Pythons And Native Colubrids In Southern Florida, United States, Steven B. Tillis, Jillian M. Josimovich, Melissa A. Miller, Laura L. Hoon-Hanks, Arik M. Hartmann, Natalie M. Claunch, Marley E. Iredale, Tracey D. Logan, Amy A. Yackel Adams, Ian A. Bartoszek, John S. Humphrey, Bryan M. Kluever, Mark D. Stenglein, Robert N. Reed, Christina M. Romagosa, James F.X. Wellehan, Robert J. Ossiboff

USDA Wildlife Services: Staff Publications

Burmese python (Python bivittatus) is an invasive snake that has significantly affected ecosystems in southern Florida, United States. Aside from direct predation and competition, invasive species can also introduce nonnative pathogens that can adversely affect native species. The subfamily Serpentovirinae (order Nidovirales) is composed of positive-sense RNA viruses primarily found in reptiles. Some serpentoviruses, such as shingleback nidovirus, are associated with mortalities in wild populations, while others, including ball python nidovirus and green tree python nidovirus can be a major cause of disease and mortality in captive animals. To determine if serpentoviruses were present in invasive Burmese …

Appraisal Of Atp1b1 And Gstm3 Proteins As Freezability Factors In Buffalo Ejaculated Spermatozoa, Marvin Bryan Segundo Salinas, Thuchadaporn Chaikhun-Marcou, Phongsakorn Chuammitri, Anucha Sathanawongs

The Thai Journal of Veterinary Medicine

Sperm cryopreservation intensifies the use of assisted reproductive technologies in food animals. However, variations in freezability confront frozen-thawed semen applications. Hence, this groundwork aimed to identify the fundamental differences between high (HF) and low freezability (LF) ejaculated spermatozoa in swamp buffaloes, which were limitedly described for this agricultural species. Twelve (12) different samples of bubaline semen were collected, cryopreserved and categorized into freezability phenotypes using post-thaw sperm motility as the basis. Sperm kinematics and functional plasma membrane integrity were evaluated in HF and LF samples using computer-assisted sperm analysis (CASA) and hypo-osmotic swelling test, respectively. The relative concentrations of some …

Impaired Cytokines Gene Expression By Diazinon And Its Relation With Blood Chemistry And Oxidative Stress Biomarkers In Nile Tilapia (Oreochromis Niloticus), Mahmoud El Sebaei, Mohammad Shathele, Sabry Mohamed El-Bahr

The Thai Journal of Veterinary Medicine

Diazinon (DZN) is a promising broad-spectrum organophosphorus insecticide. There is no evidence to support the hypothesis that DZN alters cytokine gene expression and that these modifications have an immediate impact on the histopathology, blood chemistry and oxidative stress in Nile tilapia (Oreochromis niloticus). Therefore, 225 fish were divided into one control group and four treatment groups (45 fish/group; 3 replicates/group; 15 fish/ replicate). Fish in the control group were supplied with water free of DZN. Fish in the treatment groups (T1-T4) were intoxicated with DZN at 0.28 mg/L for 1, 2, 3 and 4 weeks, respectively. Activities of AST and …

Multiplex Sybr Green Real-Time Rt-Pcr Assays For The Improved Detection Of Viral Pathogens In Horses: Equine Infectious Anemia, West Nile And Japanese Encephalitis Viruses, Sunutcha Suntrarachun, Surasak Akesowan, Suchitra Khunsup

The Thai Journal of Veterinary Medicine

Equine infectious anemia virus (EIAV), West Nile virus (WNV) and Japanese encephalitis virus (JEV) are mosquito-borne viruses that are of great concern for the equine industry worldwide. These viruses produce similar clinical signs and veterinarians need to be able to differentiate between them quickly. In this study, multiplex known as duplex and triplex SYBR Green real-time RT-PCR assays were developed to detect these three viruses and to discriminate between them based on differences in the melting temperatures (Tm) of their amplification products. Three melting peaks, generated simultaneously at temperatures of 78.5±0.5°C, 83.5±0.5°C and 85.0±1.0°C, indicated the presence of EIAV, JEV …

Effect Of Lameness On Daily Milk Yield In Dairy Cow, Piyanat Prasomsri

The Thai Journal of Veterinary Medicine

Being aware of the impact of lameness on milk production is necessary to encourage farmers to pay more attention to this issue. Therefore, we need evidence that presents the production loss due to lameness. This can be applied to make the farmers realize the importance of lameness. This study aims to examine the impact of lameness on daily milk yield based on data obtained from cow level in a large dairy farm with 2 units in Thailand. The crossbred Holstein Friesian cows were evaluated for the degree of lameness by locomotion score (LS) monthly for 8 consecutive months. Cows were …

Unravelling The Productivity Rate Of Selective Media For Brucella Strains, Mustafa Sencer Karagul, Serkan Ikiz

The Thai Journal of Veterinary Medicine

The aim of this study is to reveal the productivity rate of selective media for Brucella strains. Brucella selective media namely Farrell’s medium, CITA, Modified Thayer-Martin, Jones-Morgan medium and one additional medium including erythritol and antibiotics were compared within the scope of productivity rate and colony growth in time. The comparison was carried out using 14 strains including Brucella abortus biovars, S19 vaccine strain, Brucella suis biovars and Brucella ovis. Erythritol addition showed a positive effect on colony growth in time. In addition to differences between the productivity rates of the media; there was also some variation in the colony …

Isolation Of Drug-Sensitive Eimeria Species From Wild Turkey Feces And Development Of A Model Bioshuttle Program For Eimeria Meleagrimitis For Domestic Turkeys, Maria Carolina Trujillo Peralta

Graduate Theses and Dissertations

The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted …

Species Data And Vector Modeling: Evaluating Datasets For Improved Models Of Ixodes Ricinus Tick Distribution In Europe Under A Changing Climate, Steven Jones

Electronic Theses and Dissertations

To increase capacity for monitoring and surveillance of tick-borne diseases, publicly available tick distribution and climate change datasets are required to create accurate predictive distribution models. It is difficult, however, to assess model accuracy and utility when using incomplete datasets.  The more recent development of comprehensive tick databases for Europe and availability of climate change scenarios from multiple IPCC Assessment Reports allows for improved modeling efforts. Multiple tick datasets were combined and three climate change projections were compared by predicting current and future distributions of Ixodes ricinus ticks in Europe using the MaxEnt species distribution model. Overall, much of Europe …

Evaluation Of Ocelot And Bobcat General And Reproductive Health In South Texas And Assessment Of Assisted Reproductive Techniques For Wild Felid Conservation, Ashley Reeves

Doctoral Dissertations

Recent studies of wild felid populations in southern Texas have shown loss of genetic variability and inbreeding depression over time, increasing the risk of reduced reproductive and immune fitness leading to an overall decrease in reproductive success, increase in disease susceptibility, and further population demise. Assessment of general health, pathogen exposure, and reproductive health can provide documentation of the possible effects of inbreeding and inform the development of conservation strategies. Assisted reproductive technologies have been used in many other species and can address behavioral or physical incompatibilities among breeding pairs, connect distant populations by transporting frozen gametes, preserve genetic diversity …

Comparison Of Water-Soluble Cbd And Cbd Oil As An Anti-Inflammatory In Canines With Osteoarthritis, Sadie Sims, Tara Joiner, Dr. Laura Ken Hoffman, Barbie Papajeski

Honors College Theses

Osteoarthritis is one of the most common forms of arthritis in canines. Osteoarthritis affects the entire joint, and typically results in pain, inflammation, decreased range of motion and development of bone spurs. The purpose of this study was to provide clinical research comparing Water-Soluble Cannabidiol (CBD) and Cannabidiol (CBD) oil as an anti-inflammatory in arthritic dogs by using gait analysis on the Tekscan Strideway System, Canine Brief Pain Inventory owner surveys, and analysis of blood test results. One clinical trial was conducted with thirteen privately owned dogs. Participants were split into three treatment groups by listing them by last names …

Pulsed Electromagnetic Field Therapy And Its Applications And Usage In The Equine Industry, Delaney R. Rostad

Masters Theses

Therapeutic modalities in the equine industry are constantly progressing due to evolving needs of horse owners who desire to make their horse perform at their best. Pulsed electromagnetic field (PEMF) therapy is a lesser understood modality when it comes to its use in horse. Despite its widespread use, there is little research pertaining to how or if PEMF therapy affects performance of the equine athlete and influences various equine medical conditions; however, PEMF therapy use in humans improves delayed onset muscle soreness, and increases stride rate and length which could possibly be applied to the equine athlete. The objective of …

Nexus Between Animal Welfare, Environment, And Sustainable Development: Resource Document, Wellbeing International

Nexus – UNEP – Animal Welfare, Environment, Sustainable Development

This Resource Document has been developed to explore the Nexus (links) between Animal Welfare, the Environment, and Sustainable Development. The document includes relevant citations and reports addressing the topics encompassed by the Nexus. It will be maintained as a “living document” (subject to revision) in the WellBeing International Studies Repository. The original document and subsequent revisions will be kept in the Repository to provide a record of the changes.

Polyphenolics, Glucosinolates And Isothiocyanates Profiling Of Aerial Parts Of Nasturtium Officinale (Watercress), Sotiris Kyriakou, Kyriaki Michailidou, Tom Amery, Kyle Stewart, Paul G. Winyard, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Watercress (Nasturtium officinale) is a rich source of secondary metabolites with disease-preventing and/or health-promoting properties. Herein, we have utilized extraction procedures to isolate fractions of polyphenols, glucosinolates and isothiocyanates to determine their identification, and quantification. In doing so, we have utilized reproducible analytical methodologies based on liquid chromatography with tandem mass spectrometry by either positive or negative ion mode. Due to the instability and volatility of isothiocyanates, we followed an ammonia derivatization protocol which converts them into respective ionizable thiourea derivatives. The analytes’ content distribution map was created on watercress flowers, leaves and stems. We have demonstrated that …

Cardiac Myosin-Specific Autoimmune T Cells Contribute To Immune-Checkpoint-Inhibitor-Associated Myocarditis, Johns Hopkins University School Of Medicine, Johns Hopkins University Bloomberg School Of Public Health, Johns Hopkins University Whiting School Of Engineering, Ninaad Lasrado, Jay Reddy, Daniela Ciháková

School of Veterinary and Biomedical Sciences: Faculty Publications

Immune checkpoint inhibitors (ICIs) are an effective therapy for various cancers; however, they can induce immune-related adverse events (irAEs) as a side effect. Myocarditis is an uncommon, but fatal, irAE caused after ICI treatments. Currently, the mechanism of ICI-associated myocarditis is unclear. Here, we show the development of myocarditis in A/J mice induced by anti-PD-1 monoclonal antibody (mAb) administration alone without tumor cell inoculation, immunization, or viral infection. Mice with myocarditis have increased cardiac infiltration, elevated cardiac troponin levels, and arrhythmia. Anti-PD-1 mAb treatment also causes irAEs in other organs. Autoimmune T cells recognizing cardiac myosin are activated and increased …

Dietary Supplementation With Black Raspberries Altered The Gut Microbiome Composition In A Mouse Model Of Colitis-Associated Colorectal Cancer, Although With Differing Effects For A Healthy Versus A Western Basal Diet, Abby D. Benninghoff

Browse all Datasets

Black raspberries (BRB) are rich in anthocyanins with purported anti-inflammatory properties. However, it is not known whether dietary supplementation would ameliorate Western-diet enhanced gut inflammation and colon tumorigenesis. We employed a mouse model of colitis-associated colorectal cancer (CAC) to determine the effects of dietary supplementation with 5 to 10% (w/w) whole, freeze-dried BRB in male C57BL/6J mice fed either a standard healthy diet (AIN93G) or the total Western diet (TWD). In a pilot study, BRB suppressed colitis and colon tumorigenesis while also shifting the composition of the fecal microbiome in favor of taxa with purported health benefits, including Bifidobacteria pseudolongum. …

A Novel Vaccine Candidate Against Rabbit Hemorrhagic Disease Virus 2 (Rhdv2) Confers Protection In Domestic Rabbits, Angela M. Bosco-Lauth, Bethany Cominsky, Stephanie Porter, J. Jeffrey Root, Amber Schueler, Gary Anderson, Sara Vanderwal, Andy Benson

USDA Wildlife Services: Staff Publications

OBJECTIVE To evaluate efficacy of a novel vaccine against rabbit hemorrhagic disease virus 2 (RHDV2) in domestic rabbits.

ANIMALS 40 New Zealand White rabbits obtained from a commercial breeder.

PROCEDURES Rabbits were vaccinated and held at the production facility for the duration of the vaccination phase and transferred to Colorado State University for challenge with RHDV2. Rabbits were challenged with oral suspensions containing infectious virus and monitored for clinical disease for up to 10 days. Rabbits that died or were euthanized following infection were necropsied, and livers were evaluated for viral RNA via RT-PCR.

RESULTS None of the vaccinated animals …