Meat Science Commons^™

Comparison Of Lateral Flow To Other Pregnancy Determination Methods In Order To Determine Accuracy Of Pregnancy Status In Beef Cattle Pre And Postpartum, Adalaide C. Kline, Julie Walker, Taylor N. Andrews, Saulo Menegatti Zoca, Kaitlin M. Epperson, Lacey K. Quail, Jaclyn N. Ketchum, Jerica J.J. Rich, Jim R. Rhoades, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Blood samples were collected from six different Bos taurus herds between day 27 and 285 of gestation (heifers n = 1,205 and cows n = 1,539). Blood samples to determine PAG clearance interval were collected weekly postpartum for up to 12 weeks (heifers n = 418 and cows n = 657). Serum was tested using the lateral flow test and were read by two technicians who were blind to pregnancy status. Level of agreement between the tests were determined by Pearson’s correlation coefficients and Kappa scores. The MIXED procedure of SAS was used to evaluate the effect of …

Use Of Sperm In Vitro Capacitation And Flow Cytometry To Estimate Bull Fertility, Saulo Menegatti Zoca, Thomas W. Geary, Abigail L. Zezeski, Karl C. Kerns, Joseph C. Dalton, Bo R. Harstine, Matthew D. Utt, Robert A. Cushman, Julie Walker, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Frozen-thawed semen from five bulls previously identified as high (48.1% and 47.7%, bulls A and B, respectively), intermediary (45.5%, bull C) or low (43.1% and 40.7%, bulls D and E, respectively) fertility, based on pregnancy per AI, were evaluated with several laboratory measures. Measures included total motility, sperm plasma membrane integrity (viability), acrosome integrity, reactive oxygen species (ROS), mitochondrial membrane energy potential (mito-potential), zinc signatures (signatures 1 to 4), and CD9 protein populations at pre-wash, post-wash, h 0 (diluted with non-capacitation media), and at 0, 3, 6, and 24 h after dilution with capacitation media and incubation at …

Relationship Of Dag1 And Serpina5 Sperm Proteins With Bull Fertility, Saulo Menegatti Zoca, Julie Walker, Jerica J. J. Rich, Kaitlin M. Epperson, Taylor N. Andrews, Adalaide C. Kline, Jessica Nora Drum, M. Sofia Ortega, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Semen from 22 dairy bulls was used to evaluate the presence, localization, and quantification of DAG1 and SERPINA5 on sperm. Sperm motility parameters and viability was also evaluated for semen from each bull. Semen from 19 out of the 22 dairy bulls was used for in vitro embryo production (two Low-SCR and one High-SCR were not available for in vitro embryo production). Bulls were classified based on their sire conception rates (SCR) values as High-SCR (SCR > 1.0) or Low-SCR fertility (SCR < -4.0). Low fertility bulls were subdivided based on their blastocyst rate (BL) as High-BL (Low-SCR/High-BL BL ≥ 31%) or Low-BL (Low-SCR/Low-BL BL ≤ 26%), and High-SCR bulls were not subdivided. The GLM procedure in SAS was used with bull as a fixed effect to determine if variance was greater between bulls compared to within bull. Correlations were determined among DAG1 and SERPINA5 concentrations, percentage of tail labeled for SERPINA5, SCR, sperm total motility, progressive motility, and viability, and in vitro embryo produced cleavage rate (CL) and BL. The GLIMMIX procedure of SAS was used to evaluate the relationship of bull field fertility (High- and Low-SCR), and field and in vitro fertility (High-SCR, Low-SCR/High-BL, Low-SCR/Low-BL) classifications with sperm total (TMOT) and progressive (PROG) motility, viability, CL, BL, DAG1 and SERPINA5 relative concentration, and proportion of sperm tail labeled for SERPINA5. Both SERPINA5 and DAG1 were localized on the sperm head; however, SERPINA5 was also localized on the sperm tail. There was greater variance in concentration among bulls compared to within bull for both DAG1 (P < 0.01; 69.4 vs 49.1, respectively) and SERPINA5 (P < 0.01; 325.8 vs 285.4, respectively). There was a positive correlation between concentration of DAG1 and SERPINA5 (P = 0.01; r = 0.54). Concentrations of SERPINA5 were also correlated with CL (P = 0.04; r = 0.48), and percentage of sperm tail labeled for SERPINA5 was correlated with viability (P = 0.05; r = 0.44) and tended to be correlated with CL (P = 0.10; r = 0.39). There was no relationship between SCR or BL rate classifications and DAG1 (P ≥ 0.66), SERPINA5 (P ≥ 0.54), or percentage of sperm tail labeled for SERPINA5 (P ≥ 0.48).

Proteomic Analysis Of Epididymal And Ejaculated Sperm And Respective Fluids, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie Walker, Robert A. Cushman, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Ejaculated and epididymal semen was collected from mature Angus bulls (n = 9), and then centrifuged to separate sperm and fluid. Fluids were collected and sperm pellets were resuspended in a high ionic solution and vortexed to remove loosely attached proteins. Sperm samples were centrifuged, and the supernatant was collected. Samples collected for protein analysis were snap frozen in liquid nitrogen and stored at -80 ºC. Protein analysis was performed by liquid chromatography with tandem mass spectrometry (LCMS/MS). Yearling Angus cross bulls (n = 40) were used for sperm cultures. Ejaculated (n = 20) and epididymal (n = …

Effect Of Estrous Synchronization With Natural Service Or Fixed-Timed Artificial Insemination Using Conventional Or Gender-Kkewed Semen In Beef Females On Calving Distribution And Post Weaning Calf Performance, Julie Walker, Jerica J.J. Rich, Warren Rusche, Matthew Diersen, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Within 10 herds, beef females (n = 1,620) were either: 1) not synchronized (NonSyn) and mated to bulls, 2) synchronized (7-d controlled internal drug release (CIDR)) and mated to bulls (SynNS) 3) synchronized (7-d CO-Synch plus CIDR) and artificially inseminated with conventional semen (SynAI), or 4) synchronized (7-d CO-Synch plus CIDR) and artificially inseminated with SEXED semen. Calving distributions (calves born from d 1 to 14, 1 to 21, 22 to 42, and 43 and greater) were determined by actual birthdates and calf gender was determined at birth. Over a two-year period, a subset of calves (n = …

Genetic Parameters, Heterosis, And Breed Effects For Body Condition Score And Mature Cow Weight In Beef Cattle, André Mauric F. Ribeiro, Leticia P. Sanglard, Warren M. Snelling, R. Mark Thallman, Larry A. Kuehn, Matthew L. Spangler

Faculty Papers and Publications in Animal Science

Understanding the genetic relationship between mature cow weight (MWT) and body condition score (BCS) is useful to implement selection programs focused on cow efficiency. The objectives of this study were to estimate genetic parameters, heterosis, and breed effects for MWT and BCS. In total, 25,035 and 24,522 overlapping records were available for MWT and BCS on 6,138 and 6,131 cows, respectively, from the Germplasm Evaluation program, a crossbred beef population at the U.S. Meat Animal Research Center. Pedigree was available for 48,013 individuals. Univariate animal models were used to estimate heritabilities for each trait by parity. …

Improving Heifer Development Programs Using Precision Technology And Ddgs, Anna Dagel, Hector Menendez Iii, Jameson R. Brennan

SDSU Beef Day 2022 Summary Publication

Study Description:

Heifers were stratified into two groups based on initial body weight and each group was randomly assigned to one of two treatments: a control group (n=30) and a precision group (n=30). Treatment groups are separated into different pastures to graze dormant native range from November to April. Groups are rotated monthly to reduce individual pasture influence. Heifers in the control group are supplemented as a group and bunk fed 5lb/head/day with dried distiller grain pellets (DDGS). Heifers within the precision group will be supplemented individually using the SmartFeed system (C-Lock Inc. Rapid City, SD) and offered 5lb/head/day DDGS …

Factors Influencing Price Of South Dakota Feeder Calves Fall 2021 And Winter 2022, Adele Harty, Olivia Amundson, Robin Salverson, Kiernan Brandt, Heather Gessner, Julie Walker, Hossein Moradi Rekabdarkolaee

SDSU Beef Day 2022 Summary Publication

Study Description:

Data was collected at Faith, Philip, Hub City (Aberdeen), Mitchell, and Ft. Pierre Livestock Auctions during the weeks of October 4, 11, 18, and November 1, 2021. Data was collected and analyzed on the approximately 116,000 head of calves that were marketed during that time frame. Data collected included: auction barn, date, seller id, sex, number of head per lot, average weight, hide color, uniformity information, vaccination data, creep feed, implants, branded programs, weaned, horns, mud score, price per hundredweight, price per head, and additional comments. Uniformity score and weighted average price per hundredweight was calculated for each …

Comparing The Impact Of Continuous And Virtually Fenced Rotational Grazing On Animal Behavior And Distribution, Logan Vandermark, Jameson Brennan, Krista Ehlert, Hector Menendez Iii

SDSU Beef Day 2022 Summary Publication

Study Description:

Six herds of yearling steers (n=127) will graze native summer pastures from May to August at the Cottonwood Field Station near Philip, South Dakota. Six pastures are divided into two groups: a continuous grazing (CG) treatment with a low, moderate, and high stocking density and a virtually fenced rotational (VFR) grazing system with the same stocking densities. VenceTM virtual fence collars were placed on all animals within the study and collected GPS data at 5-minute intervals. The CG collars are not actively managed. The VFR system is actively managed through the collars and rotated based on the amount …

Using Precision Technology To Measure Cattle Methane Emissions And Intake On Western South Dakota Rangelands, Lillian J. Mcfadden, Ken Olson, Krista Ehlert, Jameson Brennan, Amanda D. Blair, Hector Menendez Iii

SDSU Beef Day 2022 Summary Publication

Study Description:

Cattle DMI is an essential component of calculating cattle stocking rates, determining nutrient requirements, and evaluating feed efficiency. Cattle DMI and digestion of forages impact enteric greenhouse gas (GHG) emissions; a major public and environmental concern. Increased GHG levels indicate energy loss during the rumen fermentation process. Obtaining data for rangeland cattle DMI and GHG emissions is needed to understand and enhance individual animal performance and reduce negative environmental impacts. We will develop enteric emissions and DMI relationships by conducting three feeding trials using the GreenFeed and SmartFeed Pro (C-Lock Inc. Rapid City, SD). The GreenFeed will measure …

Effects Of Environmental Gradients On The Abundance And Diversity Of Helminths Across 7 Bison Herds In The Upper Midwest, Bradly Wehus-Tow, Jeff M. Martin, Jameson Brennan

SDSU Beef Day 2022 Summary Publication

Study Description:

Data were collected from Wind Cave National Park and 6 satellite bison herds across the upper Midwest ranging across South Dakota, Indiana, and Kansas with a MAP gradient ranging from 34 to 82 mm and a MAT gradient ranging from 9 to 13°C. Fecal samples were collected non-invasively from 50 freshly voided bison dung patties at each herd. Helminth eggs were recovered and identified using sugar floatation; Trichostrongyle-type eggs were hatched by coproculture to identify to species. Preliminary findings indicate that general abundance of trichostrongyle-type helminths increases by 2.3 EPG per 1°C and by 0.2 EPG per mm, …

Influence Of Beef Carcass Chilling Rate On Steak Case Life And Quality Traits, Trevor Dehaan, Lydia Hite, Erin Gubbels, Christina Elaine Bakker, Amanda D. Blair, Judson Grubbs, Keith Underwood

SDSU Beef Day 2022 Summary Publication

Study Description:

Twelve head of fed beef cattle were harvested at the SDSU Meat Laboratory over two days. Carcasses were allotted into two weight groups based on hot carcass weight (HCW): Heavyweight (HW) and Lightweight (LW). Data logging thermometers were placed in the left side of each carcass within the round, loin, rib, and chuck primals to track temperature decline. Carcass measurements including 12th rib fat thickness, ribeye area and marbling score were collected approximately 48 hours postmortem. Steaks from each primal were collected to measure Warner-Bratzler shear force (WBSF), objective and subjective color.

Serving Beef In Usda Child Nutrition Programs In South Dakota: Development Of An Faq, Anna Barr, Mikayla Hardy, Andrea Alma, Heather Hopwood, Joanna Studt

SDSU Beef Day 2022 Summary Publication

Study Description:

The project occurred in partnership with four organizations representing state, tribal, and federal affiliations. The authors collaborated to compile and answer questions. Questions that were not answered by the authors’ organizations were asked to the SD Animal Industry Board and USDA Food Safety and Inspection Service.

2022 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

The purpose of beef cattle and beef product research at the University of Nebraska-Lincoln is to provide reference information that represents the various populations (cows, calves, heifers, feeders, carcasses, retail products, etc.) of beef production.

Dedicated in memory of Dr. Terry Klopfenstein (March 10, 1939-April 30, 2021), Professor Emeritus, Animal Science Department, University of Nebraska-Lincoln.

Cow-Calf Nutrition and Management

Impact of Cow Size on Economic Profitability in the Cow-Calf and Feedlot Production Systems

Comparison of Partially Confined and Traditional Cow-Calf Systems

Concepts in Stress Physiology

Inflammatory Modulators Improve Daily Gain of Heat-Stressed Wethers

Zilpaterol Supplementation Improved Indicators of Well-Being, but …

Effects Of Harvest Season On Carcass Characteristics Of Lambs In The Intermountain West, J. R. Whaley, T. W. Murphy, C. L. Gifford, W. J. Means, J. P. Ritten, H. N. Mckibben, C. M. Page, W. C. Stewart

Roman L. Hruska U.S. Meat Animal Research Center

Objective: The objectives of this study were to survey characteristics including hot carcass weight (HCW), 12th rib fat thickness (RFT), body-wall thickness (BWT), longissimus muscle area (LMA), USDA yield grade (USDA YG), percentage closely trimmed retail cuts (RC), and calculated yield grade (Calc YG) of lamb carcasses in the Intermountain West to determine the effects of season of slaughter and interrelationships among carcass characteristics.

Materials and Methods: Lamb carcass characteristics were evaluated in 2 commercial Intermountain West processing plants over one year (n = 10,027). Carcasses were evaluated by season: spring (December–April, n = 2,322) and summer (May–August, n …

Effects Of Increasing Dietary Zinc Sulfate Fed To Gestating Ewes: Ii. Milk Somatic Cell Count, Microbial Populations, And Fatty Acid Composition, C. M. Page, R. M. Knuth, T. W. Murphy, D. C. Rule, B. Bisha, J. B. Taylor, W. C. Stewart

Roman L. Hruska U.S. Meat Animal Research Center

Objective: The objective of the research was to evaluate the effects of increasing dietary Zn sulfate concentration for primiparous gestating ewes on subsequent milk SCC, intramammary microbial identifications, and fatty acid composition.

Materials and Methods: Commercial white-face (WF; n = 27) and black-face (BF; n = 24) ewes (age ≈18 mo; BW = 87.48 ± 8.37 kg) were sorted into breed-type groups and within groups ranked by BW, and then, they were randomly divided into 3 dietary supplement treatment groups: CON (n = 13; 40 mg/kg Zn; ≈1 × NASEM recommendations), Zn500 (n = 21; 500 mg/kg Zn; …

Impact Of Disclosing Fat Content On Consumer Sensory Evaluation Of Ground Beef From A Similar Source, K. R. Lybarger, K. M. Harr, E. S. Beyer, K. J. Farmer, S. G. Davis, M. D. Chao, J. L. Vipham, M. D. Zumbaugh, T. G. O'Quinn

Kansas Agricultural Experiment Station Research Reports

Objective: The objective of this study was to determine the impact of providing consumers with information regarding the fat content of ground beef on the consumer’s eating experience.

Study Description: Ground beef chubs that were 80% lean/20% fat (n = 15/panel type) were obtained, and 0.25-lb patties were fabricated from the chubs. Chubs were assigned randomly to panels. Panelists received samples labeled as the following: 90% lean/10% fat (90/10), 80% lean/20% fat (80/20), 73% lean/27% fat (73/27), lean, extra lean, and one sample with no information given (NONE). Samples were evaluated by consumers (n = 105), who were provided information …

Native Beef Collagenase Mmp-9 May Contribute To Tenderness Improvement By Degrading Connective Tissues In Extended Aged Beef, L. A. Koulicoff, A. A. Welter, P. A. Hammond, C. K. Chun, T. G. O'Quinn, G. Magnin-Bissel, M. D. Chao

Kansas Agricultural Experiment Station Research Reports

Objective: Collagen is one of the main components in the connective tissue (CT) and contributes to background toughness in beef. It is known that in living animals, collagen can be degraded and remodeled by collagenase matrix metalloproteinases (MMP); however, it is unclear if collagenase MMP can impact CT texture during postmortem aging of beef. Therefore, this study aimed to understand how collagenase MMP activity may impact postmortem connective tissue degradation in beef in three different cuts and four different aging periods.

Study Description: Beef boneless striploin, top sirloin butt, and heel were acquired from 10 U.S. Department of Agriculture high …

Changes In The Perception Of Ground Beef Quality As A Result Of Price Per Pound Labeling, E. S. Beyer, K. M. Harr, K. J. Farmer, S. G. Davis, M. D. Chao, J. L. Vipham, M. D. Zumbaugh, T. G. O'Quinn

Kansas Agricultural Experiment Station Research Reports

Objective: The objective of this study was to determine the effect of perceived palatability on ground beef patties by providing consumers with differing price per pound labels.

Study Description: Ground beef chubs (n = 15) of 80% lean/20% fat composition were used for all samples. Patties were formed 11 days after processing into 0.25 lb patties using a commercial patty former.

Samples were cooked to 160°F and served to consumers to determine different quality attributes. Consumers were given the following prices for each sample: Ultra-High - $6.25/lb; High - $5.00/lb; Medium - $3.75/lb; Low - $2.50/lb; Ultra-Low - $1.25/lb or …

Changes In The Perception Of Ground Beef Quality As A Result Of Primal Labeling, L. A. Egger, K. M. Harr, E. S. Beyer, K. J. Farmer, S. G. Davis, M. D. Chao, J. L. Vipham, M. D. Zumbaugh, T. G. O'Quinn

Kansas Agricultural Experiment Station Research Reports

Objective: The objective of this study was to determine the effect of providing primal source information to consumers prior to consumption on palatability ratings of ground beef from the same source.

Study Description: Ground beef chubs that were 80% lean and 20% fat (n = 15) were used for testing. Samples were served to consumers as 0.25 lb patties that were cooked internally to 160°F. Consumers were asked to evaluate and assess different palatability traits and evaluated samples identified as ground chuck, ground round, ground sirloin, and store ground along with a sample that offered no information.

Bottom Line: Based …