Meat Science Commons^™

Evaluating The Microbial Quality And Use Of Antimicrobials In Raw Pet Foods, Leslie Pearl Cancio

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Raw meat-based diets (RMBDs) are emerging pet foods that pose food safety risks because of the potential presence of pathogens that could cause illness to humans. In this research, the microbial quality of select RMBD products sold by pet food companies online and the use of chemical antimicrobials to reduce the microbial load in chicken liver, a common RMBD ingredient, were evaluated.

Ground meat blends and livers from four animal species (beef, pork, chicken, turkey) were purchased from four online companies that delivers directly to consumers through parcel businesses. Products were procured at three different times during one year and …

Greater Numbers Of Antral Follicles In The Ovary Are Associated With Increased Concentrations Of Glucose In Uterine Luminal Fluid Of Beef Heifers, Alexandria P. Snider, Matthew S. Crouse, Shelby L. Rosasco, Kaitlin M. Epperson, Emmalee J. Northrop-Albrecht, Jerica J.J. Rich, Chadwick C. Chase Jr., Jeremy R. Miles, George A. Perry, Adam F. Summers, Robert A. Cushman

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Increased antral follicles are associated with greater fertility and a uterine environment that is more supportive of early embryonic development in beef heifers. Glucose is a primary energy source for embryos, and glucose concentrations are elevated in uterine luminal fluid (ULF) of pregnant heifers. We hypothesized that ULF glucose concentrations and endometrial transcript abundance for glucose transporters on d16 after insemination would be greater in heifers with increased numbers of antral follicles. Heifers classified with either increased or diminished antral follicle counts were artificially inseminated following the CO-Synch protocol (d0). On d16 after insemination, reproductive tracts of heifers were collected …

Variants Within Genes Edil3 And Adgrb3 Are Associated With Divergent Fecal Egg Counts In Katahdin Sheep At Weaning, Gabrielle M. Becker, Joan M. Burke, Ronald M. Lewis, James E. Miller, James L.M. Morgan, Benjamin D. Rosen, Curtis P. Van Tassell, David R. Notter, Brenda M. Murdoch

Department of Animal Science: Faculty Publications

Gastrointestinal nematodes (GIN) pose a severe threat to sheep production worldwide. Anthelmintic drug resistance coupled with growing concern regarding potential environmental effects of drug use have demonstrated the necessity of implementing other methods of GIN control. The aim of this study was to test for genetic variants associated with resistance or susceptibility to GIN in Katahdin sheep to improve the current understanding of the genetic mechanisms responsible for host response to GIN. Linear regression and casecontrol genome-wide association studies were conducted with high-density genotype data and cube-root transformed weaning fecal egg counts (tFEC) of 583 Katahdin sheep. The casecontrol GWAS …

Proteomic Analyses Identify Differences Between Bovine Epididymal And Ejaculated Spermatozoa That Contribute To Longevity, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie A. Walker, Robert A. Cushman, George A. Perry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Sperm are stored for extended periods of time in the epididymis, but upon ejaculation motility is increased and lifespan is decreased. The objective of this study was to identify differences in proteins between epididymis and ejaculated samples that are associated with longevity. Ejaculated semen was collected from mature Angus bulls (n = 9); bulls were slaughtered and epididymal semen was collected. Epididymal and ejaculated semen were centrifuged to separate sperm and fluid. Fluids were removed and sperm pellets were resuspended in a high ionic solution and vortexed to remove loosely attached proteins. Sperm samples were centrifuged, and the supernatant was …

Untangling The Placentome Gene Network Of Beef Heifers In Early Gestation, Wellison J.S. Diniz, Lawrence P. Reynolds, Alison K. Ward, Pawel P. Borowicz, Kevin K. Sedivec, Kacie L. Mccarthy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald R. Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

The cotyledon and caruncle tissues provide a functional bridge between the fetus and the dam. However, the relationship between these tissues and the transcriptomic profile that underlies the tissue functions remains elusive. Herein we investigate the expression profile of cotyledon and caruncle from nulliparous beef heifers carrying female fetuses at day 83 of pregnancy to identify changes occurring across tissues that contribute to placental function and their tissue-specific roles. We identified 2654 differentially expressed genes [padj ≤ 0.05, abs(log2FC) ≥ 1], including nutrient transporters and paternally imprinted genes. We found key regulators of tissue function and differentiation, including FOXO4, GATA2, …

Sdsu Beef Day 2022 Complete Proceedings, Department Of Animal Science, Sdsu Extension

SDSU Beef Day 2022 Summary Publication

This is the complete proceedings of the South Dakota State University, Department of Animal Science Beef Day 2022. The proceedings contain research presented on the following topics:
Feedlot
Herd Management
Meat Science and Nutrition

Impact Of Corn Silage Moisture And/Or Kernel Processing At Harvest On Finishing Steer Growth Performance, Efficiency Of Dietary Net Energy Utilization And Carcass Traits, Thomas G. Hamilton, Warren Rusche, Julie Walker, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Red Angus steers (n = 192; initial shrunk BW = 983 ± 62.3 lbs) were used in the 112 d finishing experiment at the Southeast Research Farm (SERF) of the South Dakota Agricultural Experiment Station in Beresford. Steers were from a single source and obtained from a local SD auction facility. Steers were received 2 weeks prior to trial initiation. Steers were offered a common diet containing 60% concentrate upon arrival. Steers were transitioned to a 90% concentrate diet over the course of 14 d. Steers were consuming the finishing diet (Table 1) at the initiation of the …

Evaluation Of Dietary Roughage Inclusion In A Single Or Two-Diet System For Backgrounding And Finishing, Thomas G. Hamilton, Warren Rusche, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Pre-conditioned crossbred beef steers (n = 46; initial shrunk [4%] BW = 621 ± 89.1 lbs) were used in a 210-d grow-finish experiment at the Ruminant Nutrition Center (RNC) in Brookings, SD. Steers were fed once daily, and bunks were managed according to a slick bunk management system. Cattle were fed in 25 × 25 ft concrete surface pens (n = 10 pens; 5 pens/treatment) with 25 linear ft of bunk space and heated, concrete, continuous flow waterers. Treatments included: 1) A single-diet program (targeted a 59 Mcal/cwt NEg diet fed for 210-d; 1D) or 2) two-diet program …

Dose Effects Of Encapsulated Butyric Acid And Zinc On Beef Feedlot Steer Growth Performance, Carcass Characteristics, And Dietary Net Energy Utilization, Forest L. Francis, Erin R. Gubbels, Thomas G. Hamilton, Warren Rusche, Doug Lafleur, Jerilyn E. Hergenreder, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Steers (n = 272; shrunk BW = 794 ± 163 pounds) were assigned to one of four dietary treatments in a 143.5 d feedlot finishing trial: 0 g BPZ/ kg diet dry matter (DM) (CON), 1 g BPZ/ kg diet DM (1BPZ), 2 g BPZ/ kg diet DM (2BPZ), or 3 g BPZ/ kg diet DM (3BPZ). Carcass data and liver health outcomes were collected, and feedlot growth performance data and efficiency of dietary net energy utilization were calculated on a carcass-adjusted basis.

Evaluation Of A Direct Fed Microbial And/Or An Enzymatically Hydrolyzed Yeast Product In Diets Containing Monensin Sodium On Feedlot Phase Growth Performance, Efficiency Of Dietary Net Energy Utilization, And Carcass Characteristics In Newly Weaned Beef Steers Fed In Confinement For 258 Days, Erin Gubbels, Forest Francis, Thomas G. Hamilton, Jason Griffin, Warren Rusche, Tom Rehberger, Elliot Block, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Single-sourced, newly weaned steers (n = 256; initial body weight (BW) = 542 ± 3.7 lb) were allotted to 32 pens (n = 8 pens/treatment with 8 steers/pen). Steers were blocked by location in a 2x2 factorial treatment arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Steers were vaccinated and poured at processing and individually weighed on d 1, 14, 42 (end of receiving phase; implanted), 77, 105 (end of growing phase), 133, 161 (implanted), 182, 230 (start ractopamine HCl) and 258. Growth performance and carcass measurements were …

Evaluation Of A Direct Fed Microbial An An Enzymatically Hydrolyzed Yeast Product Fed Alone Or In Combination To Beef Steers Administered Ractopamine Hydrochloride 28 Days Prior To Harvest During Summer Months In The Northern Plains, Erin Gubbels, Forest Francis, Thomas G. Hamilton, Jason Griffin, Warren Rusche, Tom Rehberger, Elliot Block, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Single-sourced, newly weaned steers (n=256; initial BW=542 ± 3.7lb; n=64 steers/treatment; 8 steers/pen) were blocked by location in a 2×2 factorial arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Temperature-humidity index (THI) was calculated as: THI=0.81×ambient temperature+[relative humidity×(ambient temperature-14.40)]+46.40. On d-1 and 2 and d-21 and 22 on RH, respiration rate (RR) and panting scores (PS) were determined before and after AM and PM feedings (0700h, 1100h, 1400h, 1700h). RR (n=3 steers/pen) was calculated from: 600/seconds required for 10 flank movements. PS utilized this scoring system: 0 (not …

Effects Of On-Arrival Application Of A Modified-Live Respiratory And Clostridia Vaccination On Health, Growth Performance, And Antibody Titers Of Newly-Weaned Calves, Erin Gubbels, Santana Hanson, Tommy Norman, Thiago Ribeiro, Thomas G. Hamilton, Forest Francis, Jason Griffin, Warren Rusche, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Single-sourced, newly weaned steers (n=70; initial body weight (BW)=560±12.9lb) were allotted to 10 pens (n=5 pens/treatment with 7 steers/pen). Steers were blocked by BW in a randomized complete block design of VAC [vaccinated for IBR, BVD 1 and 2, PI3, and BRSV (Bovi-Shield Gold 5, Zoetis, Parsippany, NJ) and clostridial (Ultrabec 7/Somubac, Zoetis) upon arrival] or NOVAC (not vaccinated for IBR, BVD 1 and 2, PI3, and BRSV or clostridial species upon arrival). Steers were individually weighed on d 0 (arrival), 1, 21, and 42 for growth performance measures. Whole blood samples (10 mL) were collected (n=3 steers/pen …

Principles Of Holistic Resource Management: Investing In Self-Education, Pete Bauman

SDSU Beef Day 2022 Summary Publication

Study Description:

First developed as a concept for approaching grazing resources management by Alan Savory in the 1970’s as a means of combating poor grazing practices, the concept of Holistic Management has evolved in the modern era to Holistic Resource Management, or in some cases “Wholistic” Resource Management, suggesting a more inclusive approach to overall planning and assessment beyond developing a sustainable grazing plan.

In simplified terms, a complete Holistic Resource Management plan for an agricultural businesses ultimately embraces a core concept that centers on the original ‘Triple Bottom Line’ accounting philosophy where the three “P’s” of profit, people, and …

Use Of Pregnancy Associated Glycoproteins To Determine Fetal Age Throughout Gestation And Clearance Rate In Postpartum Beef Cattle, Adalaide C. Kline, Julie Walker, Taylor N. Andrews, Saulo Menegatti Zoca, Kaitlin M. Epperson, Lacey K. Quail, Jerica J.J. Rich, Jim R. Rhoades, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Previously identified pregnant females from four different herds and postpartum females from one herd were utilized. Blood samples were collected (n = 1,753; study 1) between d 28 and 285 of gestation and (heifers n = 418 and cows n = 657; study 2) once a week for up to 12 weeks after calving. Serum was tested in duplicate using a commercially available blood pregnancy test, IDEXX Alertys Pregnancy Test. In study 1, procedures were modified to allow PAG concentrations to fall within the detectible range of the assay. Data were analyzed using the MIXED procedure of SAS …

Comparison Of Lateral Flow To Other Pregnancy Determination Methods In Order To Determine Accuracy Of Pregnancy Status In Beef Cattle Pre And Postpartum, Adalaide C. Kline, Julie Walker, Taylor N. Andrews, Saulo Menegatti Zoca, Kaitlin M. Epperson, Lacey K. Quail, Jaclyn N. Ketchum, Jerica J.J. Rich, Jim R. Rhoades, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Blood samples were collected from six different Bos taurus herds between day 27 and 285 of gestation (heifers n = 1,205 and cows n = 1,539). Blood samples to determine PAG clearance interval were collected weekly postpartum for up to 12 weeks (heifers n = 418 and cows n = 657). Serum was tested using the lateral flow test and were read by two technicians who were blind to pregnancy status. Level of agreement between the tests were determined by Pearson’s correlation coefficients and Kappa scores. The MIXED procedure of SAS was used to evaluate the effect of …

Use Of Sperm In Vitro Capacitation And Flow Cytometry To Estimate Bull Fertility, Saulo Menegatti Zoca, Thomas W. Geary, Abigail L. Zezeski, Karl C. Kerns, Joseph C. Dalton, Bo R. Harstine, Matthew D. Utt, Robert A. Cushman, Julie Walker, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Frozen-thawed semen from five bulls previously identified as high (48.1% and 47.7%, bulls A and B, respectively), intermediary (45.5%, bull C) or low (43.1% and 40.7%, bulls D and E, respectively) fertility, based on pregnancy per AI, were evaluated with several laboratory measures. Measures included total motility, sperm plasma membrane integrity (viability), acrosome integrity, reactive oxygen species (ROS), mitochondrial membrane energy potential (mito-potential), zinc signatures (signatures 1 to 4), and CD9 protein populations at pre-wash, post-wash, h 0 (diluted with non-capacitation media), and at 0, 3, 6, and 24 h after dilution with capacitation media and incubation at …

Relationship Of Dag1 And Serpina5 Sperm Proteins With Bull Fertility, Saulo Menegatti Zoca, Julie Walker, Jerica J. J. Rich, Kaitlin M. Epperson, Taylor N. Andrews, Adalaide C. Kline, Jessica Nora Drum, M. Sofia Ortega, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Semen from 22 dairy bulls was used to evaluate the presence, localization, and quantification of DAG1 and SERPINA5 on sperm. Sperm motility parameters and viability was also evaluated for semen from each bull. Semen from 19 out of the 22 dairy bulls was used for in vitro embryo production (two Low-SCR and one High-SCR were not available for in vitro embryo production). Bulls were classified based on their sire conception rates (SCR) values as High-SCR (SCR > 1.0) or Low-SCR fertility (SCR < -4.0). Low fertility bulls were subdivided based on their blastocyst rate (BL) as High-BL (Low-SCR/High-BL BL ≥ 31%) or Low-BL (Low-SCR/Low-BL BL ≤ 26%), and High-SCR bulls were not subdivided. The GLM procedure in SAS was used with bull as a fixed effect to determine if variance was greater between bulls compared to within bull. Correlations were determined among DAG1 and SERPINA5 concentrations, percentage of tail labeled for SERPINA5, SCR, sperm total motility, progressive motility, and viability, and in vitro embryo produced cleavage rate (CL) and BL. The GLIMMIX procedure of SAS was used to evaluate the relationship of bull field fertility (High- and Low-SCR), and field and in vitro fertility (High-SCR, Low-SCR/High-BL, Low-SCR/Low-BL) classifications with sperm total (TMOT) and progressive (PROG) motility, viability, CL, BL, DAG1 and SERPINA5 relative concentration, and proportion of sperm tail labeled for SERPINA5. Both SERPINA5 and DAG1 were localized on the sperm head; however, SERPINA5 was also localized on the sperm tail. There was greater variance in concentration among bulls compared to within bull for both DAG1 (P < 0.01; 69.4 vs 49.1, respectively) and SERPINA5 (P < 0.01; 325.8 vs 285.4, respectively). There was a positive correlation between concentration of DAG1 and SERPINA5 (P = 0.01; r = 0.54). Concentrations of SERPINA5 were also correlated with CL (P = 0.04; r = 0.48), and percentage of sperm tail labeled for SERPINA5 was correlated with viability (P = 0.05; r = 0.44) and tended to be correlated with CL (P = 0.10; r = 0.39). There was no relationship between SCR or BL rate classifications and DAG1 (P ≥ 0.66), SERPINA5 (P ≥ 0.54), or percentage of sperm tail labeled for SERPINA5 (P ≥ 0.48).

Proteomic Analysis Of Epididymal And Ejaculated Sperm And Respective Fluids, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie Walker, Robert A. Cushman, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Ejaculated and epididymal semen was collected from mature Angus bulls (n = 9), and then centrifuged to separate sperm and fluid. Fluids were collected and sperm pellets were resuspended in a high ionic solution and vortexed to remove loosely attached proteins. Sperm samples were centrifuged, and the supernatant was collected. Samples collected for protein analysis were snap frozen in liquid nitrogen and stored at -80 ºC. Protein analysis was performed by liquid chromatography with tandem mass spectrometry (LCMS/MS). Yearling Angus cross bulls (n = 40) were used for sperm cultures. Ejaculated (n = 20) and epididymal (n = …

Genetic Parameters, Heterosis, And Breed Effects For Body Condition Score And Mature Cow Weight In Beef Cattle, André Mauric F. Ribeiro, Leticia P. Sanglard, Warren M. Snelling, R. Mark Thallman, Larry A. Kuehn, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Understanding the genetic relationship between mature cow weight (MWT) and body condition score (BCS) is useful to implement selection programs focused on cow efficiency. The objectives of this study were to estimate genetic parameters, heterosis, and breed effects for MWT and BCS. In total, 25,035 and 24,522 overlapping records were available for MWT and BCS on 6,138 and 6,131 cows, respectively, from the Germplasm Evaluation program, a crossbred beef population at the U.S. Meat Animal Research Center. Pedigree was available for 48,013 individuals. Univariate animal models were used to estimate heritabilities for each trait by parity. …

Effect Of Estrous Synchronization With Natural Service Or Fixed-Timed Artificial Insemination Using Conventional Or Gender-Kkewed Semen In Beef Females On Calving Distribution And Post Weaning Calf Performance, Julie Walker, Jerica J.J. Rich, Warren Rusche, Matthew Diersen, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Within 10 herds, beef females (n = 1,620) were either: 1) not synchronized (NonSyn) and mated to bulls, 2) synchronized (7-d controlled internal drug release (CIDR)) and mated to bulls (SynNS) 3) synchronized (7-d CO-Synch plus CIDR) and artificially inseminated with conventional semen (SynAI), or 4) synchronized (7-d CO-Synch plus CIDR) and artificially inseminated with SEXED semen. Calving distributions (calves born from d 1 to 14, 1 to 21, 22 to 42, and 43 and greater) were determined by actual birthdates and calf gender was determined at birth. Over a two-year period, a subset of calves (n = …