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Location And Conformation Of Titin In Caenorhabditis Elegans Muscle, Gaberielle S. Prince 2022 University of South Alabama

Location And Conformation Of Titin In Caenorhabditis Elegans Muscle, Gaberielle S. Prince

Theses and Dissertations

Striated muscles consist of contractile myofibrils that generate force. The repeating units (sarcomeres) of striated myofibrils contain thin & thick filaments composed of actin and myosin. In vertebrates, titin connects thin & thick filaments during myofibril assembly and produces passive tension. Invertebrate homologs of vertebrate titin are similar in structure molecularly. However, invertebrate titin homologs are smaller than vertebrate titin, and likely function differently within invertebrate muscles. Here, I used Ce-TTN-1, a titin homolog in the invertebrate roundworm C elegans. To visualize Ce-TTN-1 in C. elegans, I used Nested CRISPR gene editing to generate translational fusions of a red fluorescent protein at ...


Visualizing Muscle Assembly And Function In C. Elegans, Michael B. Russell 2022 University of South Alabama

Visualizing Muscle Assembly And Function In C. Elegans, Michael B. Russell

Theses and Dissertations

Myofibrils produce the forces that shorten muscles during contraction and are composed of proteins arranged in repeating contractile units (sarcomeres). These are composed of various proteins that self-organize into precise, alternating groups. The process of myofibril assembly is robust, flexible, and medically relevant. Various protein isoforms have evolved to modify the assembly process and generate a variety of muscle types with distinct physiologies and dynamics. To study myofibril assembly, I used the nematode Caenorhabditis elegans (C. elegans) which is an ideal model organism for this because it is transparent and has a variety of different muscle types that are responsible ...


Weaving An Interdisciplinary Microbiome Career Using Threads From Different Ecosystems, Sarah Hosler 2022 University of Maine

Weaving An Interdisciplinary Microbiome Career Using Threads From Different Ecosystems, Sarah Hosler

Electronic Theses and Dissertations

Animals have trillions of microorganisms living in or on many body sites, these communities of microorganisms are called microbiomes. Microbiomes are typically host-specific, and a lot of information about the host can be determined from investigating them. Microbiome research has many real-world applications, and this thesis utilizes the One Health perspective, which acknowledges the connection of humans, animals, and environments, and emphasizes the need for collaborative, interdisciplinary research. The first interdisciplinary project is an investigation into the bacteria in wild and cultured Atlantic deep-sea scallop, Placopecten magellanicus larvae. Adults in hatcheries can be induced to spawn, but the last two ...


Genetic Protocols For Dna Extraction From White-Tailed Deer Cast Antlers To Confirm Individuality, Zach Carter, Brian C. Peterson, Casey W. Schoenebeck, Kimberly A. Carlson 2022 University of Nebraska at Kearney

Genetic Protocols For Dna Extraction From White-Tailed Deer Cast Antlers To Confirm Individuality, Zach Carter, Brian C. Peterson, Casey W. Schoenebeck, Kimberly A. Carlson

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

White-tailed deer (Odocoileus virginianus) are the most sought-after deer species in America. The antlers of mammals, such as deer, are one of the fastest regenerative tissues in the world and are grown and naturally cast every year. Research on cast antlers have been used for a variety of purposes including population comparisons and impacts of deer health due to climatic stressors. When investigating cast antlers, it is important to confirm individuality of match sets in addition to antlers of the same individual between years. Therefore, individuality must be confirmed genetically, and protocols must be developed and established to do so ...


Utah Brand Inspections For Horses, Karl Hoopes, Jacob Hadfield, Jessie Hadfield 2022 Utah State University

Utah Brand Inspections For Horses, Karl Hoopes, Jacob Hadfield, Jessie Hadfield

All Current Publications

Brand inspection laws and proof of ownership laws are often misunderstood by horse owners in Utah. Outlined in this paper are the current laws regarding requirements for proof of ownership while traveling and brand inspection requirements during the transfer of ownership.


Validation Of Pilot Protocol: Damage Scoring Of Puget Sound Mollusk Shells, Lauren Doffing 2022 University of Washington Tacoma

Validation Of Pilot Protocol: Damage Scoring Of Puget Sound Mollusk Shells, Lauren Doffing

Environmental Science Undergraduate Theses

The Marine Sediment Monitoring Team at the Washington State Department of Ecology observed damage to mollusk shells while studying the benthic communities of Puget Sound. A pilot protocol was written to allow researchers to quantify the damage. Two researchers independently followed the protocol, including reference photos, for a set of samples collected in 2019 from an urban bay, East Possession Sound. Two scores were given to each specimen: highest-level damage and extent of highest-level damage. An additional score was given to bivalve species: rust/stain. The sets of scores were compared to determine if the protocol yielded similar values between ...


Can Financialization Save Nature? The Case Of Endangered Species, Diane-Laure Arjalies, Delphine Gibassier 2022 Ivey Business School

Can Financialization Save Nature? The Case Of Endangered Species, Diane-Laure Arjalies, Delphine Gibassier

Business Publications

The current biodiversity loss is dramatic. Over the past 50 years, more than 68% of the mammals, birds, amphibians, reptiles, and fish on earth have disappeared, putting the planet's survival and its inhabitants – including human beings – at risk (WWF, 2020). Financialization, or the transformation of nature into financial assets, is increasingly proposed as a solution to the biodiversity crisis. Proponents of financialization believe that assigning a monetary value to nature will incentivize human beings to protect habitats and their species. This article offers a four-mechanism model of nature’s financialization, explaining why it is virtually impossible to financialize nature ...


Climate-Related Declines In Reproductive Output In A High-Elevation Population Of Ground Squirrels, Kayleigh Little 2022 University of San Francisco

Climate-Related Declines In Reproductive Output In A High-Elevation Population Of Ground Squirrels, Kayleigh Little

Undergraduate Honors Theses

Beginning in the 1900s, changes in climate have resulted in an increase in global temperatures. This warming has driven behavioral and demographic changes within a multitude of species worldwide. Historically, small mammal populations have responded to changes in temperature by adjusting their geographic ranges. As temperatures rise, high-elevation populations may no longer be able to adjust as they have before. In this study, we evaluated possible effects of climate change on a high-elevation population of Belding’s ground squirrels (Urocitellus beldingi) at Tioga Pass in Northern California. We used local climate data and long-term population data from 1994 to 2021 ...


The Spatial Ecology And Habitat Selection Of A Spotted Turtle (Clemmys Guttata) Population In Southwest Michigan, Michela Coury 2022 Grand Valley State University

The Spatial Ecology And Habitat Selection Of A Spotted Turtle (Clemmys Guttata) Population In Southwest Michigan, Michela Coury

Masters Theses

Turtles are arguably the most threatened group of vertebrates on the planet. Anthropogenic influences such as habitat loss and fragmentation, road mortality, subsidized predators, climate change, and illegal collection have contributed to their global decline. Understanding which isolated populations of turtles are viable despite these synergistic threats is crucial for making conservation and management decisions. In Michigan, the spotted turtle (Clemmys guttata) is a threatened species subjected to many negative anthropogenic influences that accelerate its rapid population decline. In order to properly sustain their populations, the goal of my research was to investigate relevant factors that help us understand the ...


Reconstructing The Ecological Relationships Of Late Cretaceous Antarctic Dinosaurs And How Functional Tooth Morphology Influenced These Relationships, Ian D. Broxson 2022 Eastern Washington University

Reconstructing The Ecological Relationships Of Late Cretaceous Antarctic Dinosaurs And How Functional Tooth Morphology Influenced These Relationships, Ian D. Broxson

2022 Symposium

The Sandwich Bluff Formation of the James Ross Basin of Antarctica has recently yielded a group of five late Cretaceous dinosaurs that lived contemporaneously with each other, a first for Antarctica. These five dinosaurs include fragmentary remains of two differently sized elasmarian ornithopods, a possible megaraptor, a hadrosaur, and a nodosaur. In this study we will construct a model of the ecological relationships of late Cretaceous Antarctica. Additionally, we will look at what specific factors allowed this group of four herbivores and a carnivore to coexist in a restricted locality and what niches were filled by each species. Methods to ...


A Comprehensive Bison Management And Research Plan For The Crane Trust, Joshua D. Wiese 2022 The Platte River Whooping Crane Maintenance Trust, Inc.

A Comprehensive Bison Management And Research Plan For The Crane Trust, Joshua D. Wiese

Other Publications in Wildlife Management

The Great Plains were once a vast grassland ecosystem, but, due to agricultural and human development, are one of the most endangered ecosystems in North America. What remains is generally fragmented, threatened by invasive species, and lacks the natural ecosystem processes that shaped these grasslands such as periodic wildfire and bison grazing. Since 1978, the Platte River Whooping Crane Maintenance Trust, Inc. (dba “Crane Trust”) has worked to maintain the function of grassland and riparian habitats to benefit endangered Whooping Cranes, Sandhill Cranes, and other migratory bird species. They protect ~8,100 acres, including the largest contiguous portion of lowland ...


Pandemics And Animal Welfare: A Quantitative Inquiry Into How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Kayla N. DeSmet 2022 University of Arkansas, Fayetteville

Pandemics And Animal Welfare: A Quantitative Inquiry Into How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Kayla N. Desmet

Animal Science Undergraduate Honors Theses

Since the emergence of the COVID-19 pandemic in late 2019, industries have been impacted globally. The companion animal care industry has not received much recognition in mainstream news, and though multiple grants have been allocated (Nolen, 2020), limited resources were available to individual facilities such as animal shelters, animal day cares, and boarding facilities. Little is known about the long-term effects of the pandemic on these facilities and how companion animal care may have changed. The purpose of this study was to explore the day-to-day impacts and long-term effects of COVID-19 on the companion animal industry in Northwest Arkansas from ...


Pandemics And Animal Welfare: How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Sabrina Cox 2022 University of Arkansas, Fayetteville

Pandemics And Animal Welfare: How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Sabrina Cox

Animal Science Undergraduate Honors Theses

The first cases of coronavirus reported in the United States came in January 2020 (World Health Organization, 2020). In the two years since then, countries around the world have been affected by COVID-19 and the subsequent social, economic, and mental health issues. Previous research shows that the animal healthcare field is already overrun with burnout, substance abuse, depression, and increased levels of stress (Gardner & Hini, 2006), and the pandemic has only compounded these issues (Mair et al., 2020). Observing a lack of meaningful reporting on the issues affecting veterinary staff led the researchers to investigate staff members’ practices, day- to-day ...


Effects Of Cottonseed Meal Containing Gossypol On Testis Physiology In Boars, Kristin Ryan 2022 University of Arkansas, Fayetteville

Effects Of Cottonseed Meal Containing Gossypol On Testis Physiology In Boars, Kristin Ryan

Animal Science Undergraduate Honors Theses

Feral hogs are an invasive species found in 35 U.S. states without an effective control method to limit their population growth. According to a 2019 survey of 467 landowners in Arkansas, feral hogs caused an estimated $12 million in damages (Cook, 2019). While there are current control methods such as hunting and trapping, 65% of the feral hog population must be eliminated in a specific area to prohibit population growth (Cook, 2019). It is theorized that gossypol, a phenolic compound known to negatively impact reproductive function in other species, could cause sterility in boars. In this preliminary research study ...


Impact Of A Cattle Crossing On Water Quality Along A Tributary Of The Muddy Fork Of The Illinois River, Northwest Arkansas, Nicholas Valentini 2022 University of Arkansas, Fayetteville

Impact Of A Cattle Crossing On Water Quality Along A Tributary Of The Muddy Fork Of The Illinois River, Northwest Arkansas, Nicholas Valentini

Animal Science Undergraduate Honors Theses

Cattle are known to have an effect on water quality in various bodies of water. Studying how cattle impact water quality along various streams and tributaries is important to understanding how certain water parameters may be affected at the individual farm level. It is known that unrestricted access to a cattle crossing has been shown to increase the occurrence of downstream pollutants such as E. coli, ammonium, total kjeldahl nitrogen, total suspended solids, total phosphorus, and turbidity. However, many studies focus on large-scale operations and neglect the individual farm level. In this study, samples were collected twice for each parameter ...


Methods For Assessing Cellular Phenotypes Related To Aging And Longevity In Turtles, Stephanie E. Bulls 2022 University of South Alabama

Methods For Assessing Cellular Phenotypes Related To Aging And Longevity In Turtles, Stephanie E. Bulls

Theses and Dissertations

There are many costs associated with increased body size and longevity in animals, including the accumulation of genotoxic and cytotoxic damage that comes with having more cells and living longer. Yet, some species have overcome these barriers and have evolved remarkably large body sizes and long lifespans. Organisms with these unique phenotypes have come under recent genomic scrutiny to discover mechanisms of healthy aging and tumor suppression but little physiological work to validate these mechanisms has been conducted outside of mammals. Here I propose that reptiles, especially testudines (turtles), would be an excellent system to investigate the topics of size ...


Microplastics Exposure In At-Risk Myotis Lucifugus Bats Of The Northeastern United States, Leah Crowley 2022 University of Tennessee, Knoxville

Microplastics Exposure In At-Risk Myotis Lucifugus Bats Of The Northeastern United States, Leah Crowley

Chancellor’s Honors Program Projects

No abstract provided.


Locked Nucleic Acid Aptamer And 10 Nm Gold Nanoparticles Increases The Sensitivity Of A Prion Protein Detection Assay, Haley Channell 2022 University of Tennessee, Knoxville

Locked Nucleic Acid Aptamer And 10 Nm Gold Nanoparticles Increases The Sensitivity Of A Prion Protein Detection Assay, Haley Channell

Chancellor’s Honors Program Projects

No abstract provided.


Fragile Foal Syndrome: Its Past, Present, And Future, Alena Martin, Alena M. Martin 2022 Murray State University

Fragile Foal Syndrome: Its Past, Present, And Future, Alena Martin, Alena M. Martin

Honors College Theses

Though Fragile Foal Syndrome (FFS) has been around for some time, the public interest in FFS is recent. The hype spread rapidly from a public media announcement that a stallion at a prominent North American stud farm was a carrier (Brooks, 2021). As with most genetic mutations, FFS likely arose due to the increased inbreeding that results from human selection (Orlando & Librado, 2019). Fragile Foal Syndrome affects the enzyme responsible for developing the connective tissues. Two recent case studies highlighted the devastating effect of FFS on every part of the equine body (Metzger, et al., 2020; Grillos, 2021). So far, no one knows for sure where the mutation originated, but the strongest theory is that the origin lies with the Thoroughbred and its progenitors (Brooks, 2021). Future advances in reproductive technologies and techniques could reduce ...


Environment-Driven Shifts In Inter-Individual Variation And Phenotypic Integration Within Subnetworks Of The Mussel Transcriptome And Proteome, Richelle L. Tanner, Lani U. Gleason, W. Wesley Dowd 2022 Chapman University

Environment-Driven Shifts In Inter-Individual Variation And Phenotypic Integration Within Subnetworks Of The Mussel Transcriptome And Proteome, Richelle L. Tanner, Lani U. Gleason, W. Wesley Dowd

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The environment can alter the magnitude of phenotypic variation among individuals, potentially influencing evolutionary trajectories. However, environmental influences on variation are complex and remain understudied. Populations in heterogeneous environments might exhibit more variation, the amount of variation could differ between benign and stressful conditions, and/or variation might manifest in different ways among stages of the gene-to-protein expression cascade or among physiological functions. Here, we explore these three issues by quantifying patterns of inter-individual variation in both transcript and protein expression levels among California mussels, Mytilus californianus Conrad. Mussels were exposed to five ecologically relevant treatments that varied in the ...


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