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Measuring The Phytochemical Richness Of Meat: Effects Of Grass/Grain Finishing Systems And Grapeseed Extract Supplementation On The Fatty Acid And Phytochemical Content Of Beef, Lucas Krusinski, Isabella C. F. Maciel, Stephan van Vliet, Muhammad Ahsin, Guanqi Lu, Jason E. Rowntree, Jenifer I. Fenton 2023 Michigan State University

Measuring The Phytochemical Richness Of Meat: Effects Of Grass/Grain Finishing Systems And Grapeseed Extract Supplementation On The Fatty Acid And Phytochemical Content Of Beef, Lucas Krusinski, Isabella C. F. Maciel, Stephan Van Vliet, Muhammad Ahsin, Guanqi Lu, Jason E. Rowntree, Jenifer I. Fenton

Nutrition, Dietetics, and Food Sciences Faculty Publications

Grass-finished beef (GFB) can provide beneficial bioactive compounds to healthy diets, including omega-3 polyunsaturated fatty acids (n-3 PUFAs), conjugated linoleic acid (CLA), and secondary bioactive compounds, such as phytochemicals. The objective of this study was to compare fatty acids (FAs), micronutrients, and phytochemicals of beef fed a biodiverse pasture (GRASS), a total mixed ration (GRAIN), or a total mixed ration with 5% grapeseed extract (GRAPE). This was a two-year study involving fifty-four Red Angus steers (n = 54). GFB contained higher levels of n-3 PUFAs, vitamin E, iron, zinc, stachydrine, hippuric acid, citric acid, and succinic …


Texas A&M Beef Cattle Short Course: Program Evaluation, Nicola Oosthuizen, Pedro Fontes, Graham C. Lamb, Jason J. Cleere 2023 Texas A&M University, College Station

Texas A&M Beef Cattle Short Course: Program Evaluation, Nicola Oosthuizen, Pedro Fontes, Graham C. Lamb, Jason J. Cleere

The Journal of Extension

Survey results (n = 3,748) collected over a period of 7 years from the Texas A&M Beef Cattle Short Course (BCSC) were analyzed to evaluate course demographics and the impact of the course on attendees. Results of this survey demonstrate that attendee demographics of the BCSC are representative of beef cattle producers in the United States and that the BCSC is effective at delivering information that positively impacts beef production in Texas. Extension professionals can make use of these findings to tailor future education programs to better serve the needs of beef cattle producers nation-wide.


Broken Tails In Holstein Dairy Cattle: A Cross-Sectional Study, Hannah E. Olsen, Karly N. Anderson, Katherine C. Creutzinger, Kurt D. Vogel 2023 University of Wisconsin–River Falls

Broken Tails In Holstein Dairy Cattle: A Cross-Sectional Study, Hannah E. Olsen, Karly N. Anderson, Katherine C. Creutzinger, Kurt D. Vogel

Faculty Papers and Publications in Animal Science

Dairy cows are regularly handled when moved to the milking parlor and during other routine procedures. Low-stress handling methods are important in avoiding negative welfare states for dairy cattle. Tail twisting is used by some handlers to prompt cattle movement. However, when used inappropriately with excessive force, tail twisting can lead to a broken tail. The aim of this cross-sectional study was to determine cow-level factors that may be associated with the prevalence of broken tails in dairy cattle. A subset of 229 Holstein dairy cows (68 primiparous and 161 multiparous) at a single dairy were assessed for broken tails …


Dna Methylation Networks Underlying Mammalian Traits, Amin Haghani 2023 et al.

Dna Methylation Networks Underlying Mammalian Traits, Amin Haghani

Faculty Papers and Publications in Animal Science

INTRODUCTION: Comparative epigenomics is an emerging field that combines epigenetic signatures with phylogenetic relationships to elucidate species characteristics such as maximum life span. For this study, we generated cytosine DNA methylation (DNAm) profiles (n = 15,456) from 348mammalian species using amethylation array platform that targets highly conserved cytosines.

RATIONALE: Nature has evolved mammalian species of greatly differing life spans. To resolve the relationship ofDNAmwith maximum life span and phylogeny, we performed a largescale cross-species unsupervised analysis. Comparative studies in many species enables the identification of epigenetic correlates of maximum life span and other traits.

RESULTS: We …


Non-Destructive Evaluation Of White Striping And Microbial Spoilage Of Broiler Breast Meat Using Structured-Illumination Reflectance Imaging, Ebenezer O. Olaniyi 2023 Mississippi State University

Non-Destructive Evaluation Of White Striping And Microbial Spoilage Of Broiler Breast Meat Using Structured-Illumination Reflectance Imaging, Ebenezer O. Olaniyi

Theses and Dissertations

Manual inspection is a prevailing practice for quality assessment of poultry meat, but it is labor-intensive, tedious, and subjective. This thesis aims to assess the efficacy of an emerging structured illumination reflectance imaging (SIRI) technique with machine learning approaches for assessing WS and microbial spoilage in broiler breast meat. Broiler breast meat samples were imaged by an in house-assembled SIRI platform under sinusoidal illumination. In first experiment, handcrafted texture features were extracted from direct component (DC, corresponding to conventional uniform illumination) and amplitude component (AC, unique to the use of sinusoidal illumination) images retrieved from raw SIRI pattern images build …


Combining Host And Rumen Metagenome Profiling For Selection In Sheep: Prediction Of Methane, Feed Efficiency, Production, And Health Traits, Melanie K. Hess, Larissa Zetouni, Andrew S. Hess, Juliana Budel, Ken G. Dodds, Hannah M. Henry, Rudiger Brauning, Alan F. McCulloch, Sharon M. Hickey, Patricia L. Johnson, Sara Elmes, Janine Wing, Brooke Bryson, Kevin Knowler, Dianne Hyndman, Hayley Baird, Kathryn M. McRae, Arjan Jonker, Peter H. Janssen, John C. McEwan, Suzanne J. Rowe 2023 University Invermay Agricultural Centre, University of Nebraska-Lincoln

Combining Host And Rumen Metagenome Profiling For Selection In Sheep: Prediction Of Methane, Feed Efficiency, Production, And Health Traits, Melanie K. Hess, Larissa Zetouni, Andrew S. Hess, Juliana Budel, Ken G. Dodds, Hannah M. Henry, Rudiger Brauning, Alan F. Mcculloch, Sharon M. Hickey, Patricia L. Johnson, Sara Elmes, Janine Wing, Brooke Bryson, Kevin Knowler, Dianne Hyndman, Hayley Baird, Kathryn M. Mcrae, Arjan Jonker, Peter H. Janssen, John C. Mcewan, Suzanne J. Rowe

Faculty Papers and Publications in Animal Science

Background Rumen microbes break down complex dietary carbohydrates into energy sources for the host and are increasingly shown to be a key aspect of animal performance. Host genotypes can be combined with microbial DNA sequencing to predict performance traits or traits related to environmental impact, such as enteric methane emissions. Metagenome profiles were generated from 3139 rumen samples, collected from 1200 dual purpose ewes, using restriction enzyme-reduced representation sequencing (RE-RRS). Phenotypes were available for methane (CH4) and carbon dioxide (CO2) emissions, the ratio of CH4 to CH4 plus CO2 (CH4Ratio), feed efficiency (residual feed intake: RFI), liveweight at the time …


Effects Of Feeding A Vitamin And Mineral Supplement To Cow-Calf Pairs Grazing Native Range, Jennifer L. Hurlbert, Friederike Baumgaertner, Kacie L. McCathy, Timothy Long, Cody Wieland, Kevin K. Sedivec, Carl R. Dahlen 2023 North Dakota State University

Effects Of Feeding A Vitamin And Mineral Supplement To Cow-Calf Pairs Grazing Native Range, Jennifer L. Hurlbert, Friederike Baumgaertner, Kacie L. Mccathy, Timothy Long, Cody Wieland, Kevin K. Sedivec, Carl R. Dahlen

Faculty Papers and Publications in Animal Science

Our objectives were to evaluate the impacts of providing vitamin and mineral (VTM) supplements to cow-calf pairs during the summer grazing period on cow and calf performance and liver concentrations of minerals. During a two-year period, 727 crossbred cows and their calves (initial cow BW = 601.7 ± 48.1 kg; calf BW = 87.8 ± 5.0 kg; n = 381 in year 1, n = 346 in year 2) from the Central Grasslands Research Extension Center (Streeter, N.D.) were blocked by parity (young [parity 1 to 3], and old [parity 4+]) and randomly assigned to pastures at the beginning …


Genotype By Environment Interaction And Heteroscedasticity Influence The Expression Of Parasite Resistance In Katahdin Sheep, Brian C. Arisman, Joan M. Burke, James L. M. Morgan, Ronald M. Lewis 2023 University of Nebraska-Lincoln

Genotype By Environment Interaction And Heteroscedasticity Influence The Expression Of Parasite Resistance In Katahdin Sheep, Brian C. Arisman, Joan M. Burke, James L. M. Morgan, Ronald M. Lewis

Faculty Papers and Publications in Animal Science

Increasingly, sheep producers are choosing breeds that express resistance to gastrointestinal parasites due to reduced efficacy of anthelminthic drugs. One such breed is Katahdin. Katahdins are raised in various climates and management systems in the United States, which can be combined into eco-management clusters to describe production environments more holistically. The objectives of this study were to determine if genotype by environment interaction (G × E) and heteroscedasticity existed across these eco-management clusters for traits indicative of parasite resistance. Body weights (BW), FAMACHA scores (FAM), and fecal egg counts (FEC) were collected at around 90 d in 3,527 Katahdin lambs …


Estimating Body Weight And Body Condition Score Of Mature Beef Cows Using Depth Images, Yijie Xiong, Isabella C.F.S. Condotta, J. A. Musgrave, Tami M. Brown-Bandl, J. Travis Mulliniks 2023 University of Nebraska - Lincoln

Estimating Body Weight And Body Condition Score Of Mature Beef Cows Using Depth Images, Yijie Xiong, Isabella C.F.S. Condotta, J. A. Musgrave, Tami M. Brown-Bandl, J. Travis Mulliniks

Faculty Papers and Publications in Animal Science

Obtaining accurate body weight (BW) is crucial for management decisions yet can be a challenge for cow–calf producers. Fast-evolving technologies such as depth sensing have been identified as low-cost sensors for agricultural applications but have not been widely validated for U.S. beef cattle. This study aimed to (1) estimate the body volume of mature beef cows from depth images, (2) quantify BW and metabolic weight (MBW) from image-projected body volume, and (3) classify body condition scores (BCS) from image-obtained measurements using a machine-learning-based approach. Fifty-eight crossbred cows with a mean BW of 410.0 ± 60.3 kg and were between 4 …


Mineral Composition Of Serially Slaughtered Holstein Steers Supplemented With Zilpaterol Hydrochloride, Andrea K. Watson, Trent J. McEvers, Lee-Anne J. Walter, Nathan D. May, Jacob A. Reed, J. C. MacDonald, Galen E. Erickson, N. Andy Cole, JohnJohn P. Hutcheson, Ty E. Lawrence 2023 University of Nebraska at Lincoln

Mineral Composition Of Serially Slaughtered Holstein Steers Supplemented With Zilpaterol Hydrochloride, Andrea K. Watson, Trent J. Mcevers, Lee-Anne J. Walter, Nathan D. May, Jacob A. Reed, J. C. Macdonald, Galen E. Erickson, N. Andy Cole, Johnjohn P. Hutcheson, Ty E. Lawrence

Faculty Papers and Publications in Animal Science

Calf-fed Holstein steers (n = 115; 449 ± 20 kg) were utilized in a serial harvest experiment. A baseline group of five steers was harvested after 226 d on feed (DOF), which was designated day 0. The remaining cattle were assigned randomly to 11 harvest groups, with slaughter every 28 d. Cattle were either not (CON) or were fed zilpaterol hydrochloride for 20 d followed by a 3 d withdrawal (ZH). There were five steers per treatment in each slaughter group ranging from days 28 to 308. Whole carcasses were divided into lean, …


Effect Of Vitamin C And Probiotics On Broilers Performance And Claudin-2 (Cldn-2) Expression Under Heat Stress [Research Note], Mohammad Chamani, Mohammad Chamani, Farhad Foroudi, Ali Asghar Sadeghi, Mehdi Aminafshar 2023 Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

Effect Of Vitamin C And Probiotics On Broilers Performance And Claudin-2 (Cldn-2) Expression Under Heat Stress [Research Note], Mohammad Chamani, Mohammad Chamani, Farhad Foroudi, Ali Asghar Sadeghi, Mehdi Aminafshar

The Philippine Agricultural Scientist

This study was conducted to investigate the effect of vitamin C and probiotic which consists of five species of beneficial bacteria and two species of fungi on performance, blood parameters, and intestinal CLDN-2 gene expression of broilers during heat stress (HS). A total of 400 broilers were randomly divided into five groups, each of which received a different treatment. The first group was considered a negative control and did not experience any HS (C-); the second group was considered a positive control (C+) and was exposed to HS; the third group received only vitamin C (250 …


Impact Of Cattle Grazing Corn Residue In Spring On Soil And Subsequent Soybean Yield, Morgan T. Grabau, J. C. MacDonald, Zach E. Carlson, M. E. Drewnoski 2023 University of Nebraska, Lincoln

Impact Of Cattle Grazing Corn Residue In Spring On Soil And Subsequent Soybean Yield, Morgan T. Grabau, J. C. Macdonald, Zach E. Carlson, M. E. Drewnoski

Faculty Papers and Publications in Animal Science

The effects of spring corn (Zea mays) residue grazing and stocking density on soil physical properties and subsequent soybean (Glycine max) yield were evaluated in an experiment with three treatments: no grazing (NG), normal stocking density (NSD), and high stocking density (HSD). The study was conducted over 2 years with four replicates per treatment each year. Steers (277 ± 4.4 kg) were stocked at 7.5 steers/ha for NSD with a target grazing of 45 days starting in mid- February, and 22.5 steers/ha for HSD with a target grazing of 15 days in mid-March, such that the …


Impact Of Shade In Beef Feedyards On Performance, Ear Temperature, And Heat Stress Measures, Thomas M. Winders, Brett A. Melton, Boyd M. Boyd, Casey N. Macken, J. C. MacDonald, Galen E. Erickson 2023 University of Nebraska-Lincoln

Impact Of Shade In Beef Feedyards On Performance, Ear Temperature, And Heat Stress Measures, Thomas M. Winders, Brett A. Melton, Boyd M. Boyd, Casey N. Macken, J. C. Macdonald, Galen E. Erickson

Faculty Papers and Publications in Animal Science

A 2-yr study (year 1: March to September 2017; year 2: February to August 2018) was conducted using crossbred steers (year 1: n = 1677; initial body weight [BW] = 372 kg, SD = 47; year 2: n = 1713; initial BW = 379 kg, SD = 10) in a commercial feedyard study in Eastern NE to determine the effects of shade on cattle performance, ear temperature, and cattle activity. Two treatments were evaluated using a randomized complete block design (n = 5 blocks based on arrival). Treatments were assigned randomly to pens and consisted of five pens without …


Prostaglandin F2Α Regulates Mitochondrial Dynamics And Mitophagy In The Bovine Corpus Luteum, Michele R. Plewes, Emilia Przygrodzka, Corrine F. Monaco, Alexandria P. Snider, Jessica Jessica Keane, Patrick D. Burns, Jennifer R. Wood, Andrea S. Cupp, John S. Davis 2023 University of Nebraska Medical Center, U.S Department of Veterans Affairs Nebraska Western Iowa Health Care System

Prostaglandin F2Α Regulates Mitochondrial Dynamics And Mitophagy In The Bovine Corpus Luteum, Michele R. Plewes, Emilia Przygrodzka, Corrine F. Monaco, Alexandria P. Snider, Jessica Jessica Keane, Patrick D. Burns, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Faculty Papers and Publications in Animal Science

Prostaglandins are arachidonic acid-derived lipid mediators involved in numerous physiological and pathological processes. PGF2α analogues are therapeutically used for regulating mammalian reproductive cycles and blood pressure, inducing term labor, and treating ocular disorders. PGF2α exerts effects via activation of calcium and PKC signaling, however, little is known about the cellular events imposed by PGF2α signaling. Here, we explored the early effects of PGF2α on mitochondrial dynamics and mitophagy in the bovine corpus luteum employing relevant and well characterized in vivo and in vitro approaches. We identified PKC/ERK and AMPK as critical protein kinases essential for activation of mitochondrial fission proteins, …


Utilizing NuTrack To Access The Activity Levels In Pigs With Varying Degrees Of Genetic Potential For Growth And Feed Intake, Dalton Obermier, Melanie Trenahile-Grannemann, Ty B. Schmidt, Tom Rathje, Benny Mote 2023 University of Nebraska-Lincoln

Utilizing NuTrack To Access The Activity Levels In Pigs With Varying Degrees Of Genetic Potential For Growth And Feed Intake, Dalton Obermier, Melanie Trenahile-Grannemann, Ty B. Schmidt, Tom Rathje, Benny Mote

Faculty Papers and Publications in Animal Science

Feed cost accounts for over two-thirds of the variable cost of production. In order to reduce feed costs without sacrificing production numbers, feed efficiency must be improved. Calorie expenditure has been difficult to quantify in the past but is understood to impact residual feed intake (RFI) greatly. The objective of this work was to utilize an advanced computer vision system to evaluate activity levels across sex and sire groups with different expected breeding value combinations for growth and feed intake. A total of 199 pigs from four different sire groups (DNA Genetics Line 600) High Feed Intake/High Growth (HIHG), Low …


Determining The Effectiveness Of Rosemary Essential Oil On The Shelf Life Of Ground Beef Under Different Lighting Conditions, Jordan Looper 2023 University of Arkansas, Fayetteville

Determining The Effectiveness Of Rosemary Essential Oil On The Shelf Life Of Ground Beef Under Different Lighting Conditions, Jordan Looper

Animal Science Undergraduate Honors Theses

The objective of this study was to determine the effectiveness of rosemary extract on the shelf life of ground beef patties under different retail display conditions. Ground beef patties were produced from an 85% lean and 15% fat blend purchased from a local grocery retailer, finely ground through a 0.953 cm plate and separated into 151.2 g patties (n=64). Patties were randomly assigned into a control group (no antioxidants added) or a 0.20% concentration rosemary extract group. Patties were individually packaged using polyvinyl chloride overwrap. Antioxidant and control patties were randomly assigned into one of two lighting intensity …


A New Method For Reducing Salmonella In Poultry Meat, Regis Yizerwe 2023 University of Nebraska - Lincoln

A New Method For Reducing Salmonella In Poultry Meat, Regis Yizerwe

Honors Theses

An important approach to decreasing the transmission of Salmonella into the food chain is to treat raw chicken after processing. This can be, and has been done, by using antibiotics and chemical disinfectants. Using antibiotics is not a viable option due to the issue of antimicrobial resistance. Although some chemical disinfectants have been used effectively, they are restricted due to the amounts that can be used safely, the acceptance of their use and resistance to implementation in various parts of the world. This study has revealed that using a naturally occurring saponin–Quillaja Saponaria– in small concentrations under certain conditions of …


Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. McCathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen 2023 Auburn University

Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Faculty Papers and Publications in Animal Science

Herein, we present a dataset based on the RNA-Seq analysis of liver tissue from bovine female fetuses at day 83 of gestation. The findings were reported in the main article, “Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes”[1] . These data were generated to investigate the effects of periconceptual maternal vitamin and mineral supplementation and rates of body weight gain on the transcript abundance of genes associated with fetal hepatic metabolism and function. To this end, crossbred Angus beef heifers ( n = 35) were randomly assigned to 1 of 4 treatments in a 2 × …


Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. McCathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen 2023 Auburn University

Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Faculty Papers and Publications in Animal Science

Herein, we present a dataset based on the RNA-Seq analysis of liver tissue from bovine female fetuses at day 83 of gestation. The findings were reported in the main article, “Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes”[1] . These data were generated to investigate the effects of periconceptual maternal vitamin and mineral supplementation and rates of body weight gain on the transcript abundance of genes associated with fetal hepatic metabolism and function. To this end, crossbred Angus beef heifers ( n = 35) were randomly assigned to 1 of 4 treatments in a 2 ×2 …


Evaluating Draft Epa Emissions Models For Laying Hen Facilities, Yijie Xiong, Guoming Li, Brett C. Ramirez, Robert T. Burns, Richard S. Gates 2023 University of Nebraska - Lincoln

Evaluating Draft Epa Emissions Models For Laying Hen Facilities, Yijie Xiong, Guoming Li, Brett C. Ramirez, Robert T. Burns, Richard S. Gates

Faculty Papers and Publications in Animal Science

In August 2021, the U.S. Environmental Protection Agency (EPA) released draft models to estimate daily NH3, H2S, PM10, PM2.5, and TSP emissions from egg-layer houses (high-rise and manure-belt) and manure storage using inputs of daily mean ambient temperature, relative humidity (RH), and hen inventory. These models were developed from refined datasets generated by the National Air Emissions Monitoring Study fieldwork completed in 2009. Notably, they do not include data for cage-free housing. Currently, 66% of U.S. laying hens are housed in cages; thus, these models, if adopted, will have a substantial impact …


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