Meat Science Commons^™

Microbial Community Structure From Southern High Plains Beef Cattle Feedyard Manure And Relationship With Nitrous Oxide Emissions, Heidi Waldrip, David Parker, Sierra Miller, Lisa M. Durso, Byeng R. Min, Daniel N. Miller, Kenneth Casey, Bryan Woodbury, Mindy J. Spiehs

Roman L. Hruska U.S. Meat Animal Research Center

Modern molecular techniques enable characterization of the microbial biome in livestock manure, from which there is particular concern over emission of greenhouse gases. This study evaluated how sampling depth, time, temperature, and artificial rainfall affected microbial community structure in feedyard manure, and relationships between the manure biome and known parameters related to nitrous oxide (N₂O) emissions. In three large incubation chambers, maintained at different temperatures that received two applications of artificial rainfall, we evaluated manure microbiome composition and abundance of N₂O-producing enzymes (nirK and nirS) using quantitative polymerase chain reaction (qPCR). These data were …

The Effects Of Administering Different Metaphylactic Antimicrobials On Growth Performance And Health Outcomes Of High-Risk, Newly Received Feedlot Steers, Carley M. Coppin, Taylor M. Smock, Cory L. Helmuth, Jeff L. Manahan, Nathan S. Long, Ashley A. Hoffman, Jeffery A. Carroll, Paul R. Broadway, Nicole C. Burdick Sanchez, James E. Wells, Samodha C. Fernando, Kristin E. Hales

Faculty Papers and Publications in Animal Science

Bovine respiratory disease (BRD) is the primary animal health concern facing feedlot producers. Many antimicrobial mitigation strategies are available, but few studies have compared feedlot performance during both the receiving and finishing periods following application of different antimicrobials used as metaphylaxis at arrival. The objective of this study was to compare antimicrobial metaphylaxis methods on clinical health and growth performance across both the receiving and finishing periods. A total of 238 multiple-sourced steers in two source blocks were used in a generalized complete block design. The four treatments included: 1) a negative control, 5 mL of sterile saline injected subcutaneously …

Evaluation Of The Effects Of Wood-Sourced Biochar As A Feedlot Pen Surface Amendment On Manure Nutrient Capture, Jessica L. Sperber, Galen E. Erickson, Andrea K. Watson

Faculty Papers and Publications in Animal Science

Feedstuffs utilized in U.S. feedlot finishing rations incorporate high concentrations of N and P, with less than 15% of fed N and P retained by the animal. The remaining N and P are excreted in the manure, where the opportunity for manure N loss via ammonia (NH3) volatilization from the feedlot pen surface is a risk to the environment and lowers the value of manure as a fertilizer. Two nutrient mass balance experiments were conducted during the winter and summer seasons to evaluate the effects of spreading unprocessed Eastern red cedar biochar onto the feedlot pen surface on manure nutrient …

Type 2 Polysaccharide Storage Myopathy In Quarter Horses Is A Novel Glycogen Storage Disease Causing Exertional Rhabdomyolysis, Stephanie J. Valberg, Zoë J. Williams, Carrie J. Finno, Abigail Schultz, Deborah Velez-Irizarry, Marisa L. Henry, Keri Gardner, Jessica Lynn Petersen

Faculty Papers and Publications in Animal Science

Background: Both type 1 (PSSM1) and type 2 polysaccharide storage myopathy (PSSM2) are characterised by aggregates of abnormal polysaccharide in skeletal muscle. Whereas the genetic basis for PSSM1 is known (R309H GYS1), the cause of PSSM2 in Quarter Horses (PSSM2-QH) is unknown and glycogen concentrations not defined.

Objectives: To characterise the histopathological and biochemical features of PSSM2-QH and determine if an associated monogenic variant exists in genes known to cause glycogenosis.

Study design: Retrospective case control.

Methods: Sixty-four PSSM2-QH, 30 PSSM1-QH and 185 control-QH were identified from a biopsy repository and clinical data, histopathology scores …

Primary Myoblasts From Intrauterine Growth-Restricted Fetal Sheep Exhibit Intrinsic Dysfunction Of Proliferation And Differentiation That Coincides With Enrichment Of Inflammatory Cytokine Signaling Pathways, Robert J. Posont, Micah S. Most, Caitlin Cadaret, Eileen Marks-Nelson, Kiristen A. Beede, Sean W. Limesand, Ty B. Schmidt, Jessica L. Petersen, Dustin T. Yates

Faculty Papers and Publications in Animal Science

Intrauterine growth restriction (IUGR) is linked to lifelong reductions in muscle mass due to intrinsic functional deficits in myoblasts, but the mechanisms underlying these deficits are not known. Our objective was to determine if the deficits were associated with changes in inflammatory and adrenergic regulation of IUGR myoblasts, as was previously observed in IUGR muscle. Primary myoblasts were isolated from IUGR fetal sheep produced by hyperthermia-induced placental insufficiency (PI-IUGR; n = 9) and their controls (n = 9) and from IUGR fetal sheep produced by maternofetal inflammation (MI-IUGR; n = 6) and their …

Vitamin And Mineral Supplementation And Rate Of Weight Gain During The First Trimester Of Gestation In Beef Heifers Alters The Fetal Liver Amino Acid, Carbohydrate, And Energy Profile At Day 83 Of Gestation, Matthew S. Crouse, Kacie L. Mccarthy, Ana Clara B. Menezes, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri Dorsam, Tammi L. Neville, Alison K. Ward, Pawel P. Borowicz, Lawrence P. Reynolds, Kevin K. Sedivec, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Faculty Papers and Publications in Animal Science

The objective of this study was to evaluate the effects of feeding heifers a vitamin and mineral supplement and targeting divergent rates of weight gain during early gestation on the fetal liver amino acid, carbohydrate, and energy profile at d 83 of gestation. Seventy-two crossbred Angus heifers were randomly assigned in a 2 × 2 factorial arrangement to one of four treatments comprising the main effects of vitamin and mineral supplementation (VTM or NOVTM) and feeding to achieve different rates of weight gain (low gain [LG] 0.28 kg/day vs. moderate gain [MG] 0.79 kg/day). Thirty-five gestating heifers with female fetuses …

Vitamin And Mineral Supplementation And Rate Of Gain In Beef Heifers Ii: Effects On Concentration Of Trace Minerals In Maternal Liver And Fetal Liver, Muscle, Allantoic, And Amniotic Fluids At Day 83 Of Gestation, Kacie L. Mccarthy, Ana Clara Ana, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, Alison K. Ward, Pawel P. Borowicz, Lawrence P. Reynolds, Kevin K. Sedivec, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen, Kacie L. Mccarthy

Faculty Papers and Publications in Animal Science

We evaluated the effects of vitamin and mineral supplementation (from pre-breeding to day 83 of gestation) and two rates of gain (from breeding to day 83 of gestation) on trace mineral concentrations in maternal and fetal liver, fetal muscle, and allantoic (ALF) and amniotic (AMF) fluids. Crossbred Angus heifers (n = 35; BW = 359.5 ± 7.1 kg) were randomly assigned to one of two vitamin and mineral supplementation treatments (VMSUP; supplemented (VTM) vs. unsupplemented (NoVTM)). The VMSUP factor was initiated 71 to 148 d before artificial insemination (AI), allowing time for the mineral status of heifers to be altered …

Yearling Bulls Have Reduced Sperm Concentration And Increased Seminal Plasma Interleukin-8 After A 28-Day Breeding Season, Alexandria P. Snider, Kacie L. Mccathy, Elane C. Wright-Johnson, Jeffrey D. Ondrak, William T. Oliver, Carl R. Dahlen, Robert A. Cushman, Matthew S. Crouse

Faculty Papers and Publications in Animal Science

We hypothesized that yearling bulls selected for a 28-d breeding season would have reduced sperm concentrations and morphology, and have increased seminal plasma concentrations of pro-inflammatory cytokine interleukin-8 (IL-8). Yearling bulls were selected based on a breeding soundness examination (BSE) at approximately 415 d of age and contained at least 750 million sperm in the ejaculate, with 12 bulls randomly selected for breeding (BREEDERS) and 12 bulls not selected for breeding (NON-BREEDERS). After a 28-d breeding period, all bulls underwent a BSE. Plasma and seminal plasma were collected at each time point for analysis. Data were analysed utilizing either the …

The Role Of Microbiota On Campylobacter Jejuni Colonization And Growth Performance In Broiler Chickens, Ayidh Almansour

Graduate Theses and Dissertations

The successful poultry production at modern era comes from the vertical integratedindustrialization, which has fundamentally changed how animals have been living for millions of years. Antimicrobial growth promoters have been used to sustain the efficient industrialized animal production, driving antimicrobial overuse and resistance. Because of the increasing pressure from consumer’s concerns and government regulations, it is urgent to develop antimicrobial free alternatives as growth promoters in poultry production, but few effective antimicrobial alternatives are currently available. Campylobacter jejuni is one of the worldwide prevalent foodborne bacterial pathogens mainly transmitted from poultry. However, few mechanisms are available on why C. jejuni …

Estimates Of Daily Oxygen Consumption, Carbon Dioxide And Methane Emissions, And Heat Production For Beef And Dairy Cattle Using Spot Gas Sampling, C. Lee, K. A. Beauchemin, J. Dijkstra, D. L. Morris, K. Nichols, Paul J. Kononoff, D. Vyas

Faculty Papers and Publications in Animal Science

A simulation study was conducted to examine accuracy of estimating daily O₂ consumption, CO₂ and CH₄ emissions, and heat production (HP) using a spot sampling technique and to determine optimal spot sampling frequency (FQ). Data were obtained from 3 experiments where daily O2 consumption, emissions of CO2 and CH4, and HP were measured using indirect calorimetry (respiration chamber or headbox system). Experiment 1 used 8 beef heifers (ad libitum feeding; gaseous exchanges measured every 30 min over 3 d in respiration chambers); Experiment 2 used 56 lactating Holstein-Friesian cows (restricted feeding; gaseous exchanges measured every 12 min …

Evaluating The Shelf Life And Sensory Properties Of Beef Steaks From Cattle Raised On Different Grass Feeding Systems In The Western United States, Toni L. Duarte, Bakytzhan Bolkenov, Sarah C. Klopatek, James W. Oltjen, D. Andy King, Steven D. Shackelford, Tommy L. Wheeler, Xiang Yang

Roman L. Hruska U.S. Meat Animal Research Center

Consumer interest in grass-fed beef has been steadily rising due to consumer perception of its potential benefits. This interest has led to a growing demand for niche market beef, particularly in the western United States. Therefore, the objective of this study was to assess the impact of feeding systems on the change in microbial counts, color, and lipid oxidation of steaks during retail display, and on their sensory attributes. The systems included: conventional grain-fed (CON), 20 months-grass-fed (20GF), 25-months-grass-fed (25GF) and 20-months-grass-fed + 45-day-grain-fed (45GR). The results indicate that steaks in the 20GF group displayed a darker lean and fat …

Vitamin And Mineral Supplementation And Rate Of Gain In Beef Heifers I: Effects On Dam Hormonal And Metabolic Status, Fetal Tissue And Organ Mass, And Concentration Of Glucose And Fructose In Fetal Fluids At D 83 Of Gestation, Ana Clara B. Menezes, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, Alison K. Ward, Pawel P. Borowicz, Lawrence P. Reynolds, Kevin K. Sedivec, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Faculty Papers and Publications in Animal Science

Thirty-five crossbred Angus heifers (initial BW = 359.5 ± 7.1 kg) were randomly assigned to a 2 × 2 factorial design to evaluate effects of vitamin and mineral supplementation [VMSUP; supplemented (VTM) vs. unsupplemented (NoVTM)] and different rates of gain [GAIN; low gain (LG), 0.28 kg/d, vs. moderate gain (MG), 0.79 kg/d] during the first 83 d of gestation on dam hormone and metabolic status, fetal tissue and organ mass, and concentration of glucose and fructose in fetal fluids. The VMSUP was initiated 71 to 148 d before artificial insemination (AI), allowing time for mineral status of heifers to be …

Breeding Sustainable Beef Cows: Reducing Weight And Increasing Productivity, Warren M. Snelling, R. Mark Thallman, Matthew L. Spangler, Larry A. Kuehn

Faculty Papers and Publications in Animal Science

Programs for sustainable beef production are established, but the specific role of beef cows in these systems is not well defined. This work characterized cows for two traits related to sustainability, cow weight (CW) and cumulative weight weaned (WtW). Cow weight indicates nutrient requirements and enteric methane emissions. Cumulative weight weaned reflects reproductive performance and avoidance of premature culling for characteristics related to animal health, welfare, and worker safety. Both traits were evaluated with random regression models with records from a crossbred population representing 18 breeds that conduct US national cattle evaluations. The genomic REML analyses included additive and dominance …

Beta-Adrenergic Agonists Alter Oxidative Phosphorylation In Primary Myoblasts (Short Communication), Renae Sieck, Leah K. Treffer, Anna M. Fuller, Martonio Ponte Viana, Oleh Khalimonchuk, Ty B. Schmidt, Dustin T. Yates, Jessica Lynn Petersen

Faculty Papers and Publications in Animal Science

Beta-adrenergic agonists (β-AAs) are widely used supplements in beef and pork production to improve feed efficiency and increase lean muscle mass, yet little is known about the molecular mechanism by which β-AAs achieve this outcome. Our objective was to identify the influence of ractopamine HCl and zilpaterol HCl on mitochondrial respiratory activity in muscle satellite cells isolated from crossbred beef steers (N = 5), crossbred barrows (N = 2), Yorkshire-cross gilts (N = 3), and commercial weather lambs (N = 5). Real-time measurements of oxygen con­sumption rates (OCRs) were recorded using extracellular …

Metaphylactic Antimicrobial Effects On Occurrences Of Antimicrobial Resistance In Salmonella Enterica, Escherichia Coli And Enterococcus Spp. Measured Longitudinally From Feedlot Arrival To Harvest In High-Risk Beef Cattle, Nathan S. Long, James E. Wells, Elaine D. Berry, Jerrad F. Legako, Dale R. Woerner, Guy H. Loneragan, Paul R. Broadway, Jeff A. Carroll, Nicole C. Burdick Sanchez, Samodha C. Fernando, Carley M. Bacon, Cory L. Helmuth, Taylor M. Smock, Jeff L. Manahan, Ashley A. Hoffman, Kristin E. Hales

Faculty Papers and Publications in Animal Science

Aims: Our objective was to determine how injectable antimicrobials affected populations of Salmonella enterica, Escherichia coli and Enterococcus spp. in feedlot cattle.

Methods and Results: Two arrival date blocks of high-risk crossbred beef cattle (n = 249; mean BW = 244 kg) were randomly assigned one of four antimicrobial treatments administered on day 0: sterile saline control (CON), tulathromycin (TUL), ceftiofur (CEF) or florfenicol (FLR). Faecal samples were collected on days 0, 28, 56, 112, 182 and study end (day 252 for block 1 and day 242 for block 2). Hide swabs and subiliac …

Lc-Qtof-Ms Evaluation Of Rabbit-Specific Peptide Markers For Meat Quantitation, Anna Stachniuk, Anna Kozub, Renata Czeczko, Magdalena Montowska, Emilia Fornal

Journal of Food and Drug Analysis

Ten rabbit-specific tryptic peptide markers and one marker peptide specific to both rabbit and hare were evaluated for mass signal linearity in binary meat mixtures using liquid chromatography-quadrupole time-of-flight-mass spectrometry. Seven meat mixtures containing chicken and varying percentages of rabbit (1%, 5%, 10%, 30%, 60%, 90%, and 100%) were analyzed. Additionally, the signal linearity of twelve peptide markers for chicken meat was examined. The best candidate peptides for the quantification of meat content were selected. Five of eleven peptides for rabbit meat and five of twelve peptides for chicken meat showed good linearity (R2 > 0.97). The limits of detection and …

Genes Involved In Feed Efficiency Identified In A Meta-Analysis Of Rumen Tissue From Two Populations Of Beef Steers, Amanda K. Lindholm-Perry, Allison M. Meyer, Rebecca J. Kern-Lunbery, Hannah C. Cunningham-Hollinger, Taran H. Funk, Brittney N. Keel

Roman L. Hruska U.S. Meat Animal Research Center

In cattle, the rumen is an important site for the absorption of feed by-products released by bacterial fermentation, and variation in ruminal function plays a role in cattle feed efficiency. Studies evaluating gene expression in the rumen tissue have been performed prior to this. However, validating the expression of genes identified in additional cattle populations has been challenging. The purpose of this study was to perform a meta-analysis of the ruminal transcriptome of two unrelated populations of animals to identify genes that are involved in feed efficiency across populations. RNAseq data from animals with high and low residual feed intake …

Prenatal Transportation Stress Did Not Impact Ovarian Follicle Count For Three Generations Of Female Brahman Offspring, Lacey K. Quail, Ronald D. Randel, Thomas H. Welsh Jr., Robert A. Cushman, Hannah K. Yake, Rui A. D'Orey Branco, Donald A. Neuendorff, Charles R. Long, George A. Perry

Roman L. Hruska U.S. Meat Animal Research Center

As prenatal transportation stress altered behavior and adrenal glucocorticoid secretion of calves, we hypothesized that prenatal transportation stress would decrease ovarian reserve size and negatively impact female offspring fertility. The impact of prenatal transportation stress on ovarian follicle numbers in female offspring for three generations was studied. Brahman cows were transported for 2 h on day 60 ± 5, 80 ± 5, 100 ± 5, 120 ± 5, and 140 ± 5 of gestation. Ovaries were collected from offspring of transported or non-transported dams at multiple ages. Primordial, primary, secondary, and antral follicles were histologically analyzed. Antral follicle numbers were …

Efficacy Of Antimicrobial Interventions Used In Meat Processing Plants Against Antimicrobial Tolerant Non–Antibiotic-Resistant And Antibiotic-Resistant Salmonella On Fresh Beef, Norasak Kalchayanand, Sapna Chitlapilly Dass, Yangjunna Zhang, Eric L. Oliver, Bing Wang, Tommy L. Wheeler

Roman L. Hruska U.S. Meat Animal Research Center

Salmonella is a common cause of foodborne illness in the United States, and several strains of Salmonella have been identified as resistant to antibiotics. It is not known whether strains that are antibiotic resistant (ABR) and that have some tolerance to antimicrobial compounds are also able to resist the inactivation effects of antimicrobial interventions used in fresh meat processing. Sixty-eight Salmonella isolates (non-ABR and ABR strains) were treated with half concentrations of lactic acid (LA), peracetic acid (PAA), and cetylpyridinium chloride (CPC), which are used in beef processing plants to screen for tolerant strains. Six strains each from non-ABR and …

Using Pooled Data For Genomic Prediction In A Bivariate Framework With Missing Data, Johnna L. Baller, Stephen D. Kachman, Larry A. Kuehn, Matthew L. Spangler

Faculty Papers and Publications in Animal Science

Pooling samples to derive group genotypes can enable the economically efficient use of commercial animals within genetic evaluations. To test a multivariate framework for genetic evaluations using pooled data, simulation was used to mimic a beef cattle population including two moderately heritable traits with varying genetic correlations, genotypes and pedigree data. There were 15 generations (n = 32,000; random selection and mating), and the last generation was subjected to genotyping through pooling. Missing records were induced in two ways: (a) sequential culling and (b) random missing records. Gaps in genotyping were also explored whereby genotyping occurred through generation 13 …