Dairy Science Commons^™

Profile Of Antimicrobial Susceptibility From Cattles’S Milk Isolates Suffering From Mastitis In District Lahore, Muhammad Imran, Iqra Rehman, Abdul Qayyum Khan Sulehria, Yasser Mustafa Butt, Abdul Majid Khan, Azizah Ziauddin

Journal of Bioresource Management

Mastitis is highly prevalent infection in cattle causing cost-effective loss in dairy milk production. Escherichia coli is the most frequently isolated bacteria causing mastitis worldwide. The current study was performed to investigate the mastitis prevalence and effect of different antibiotics against pathogens causing it. In sum, 216 milk samples were collected randomly including 108 each from both cows and buffaloes. These samples were subjected to Surf Field Mastitis Test for prevalence of mastitis that found 18.50% and 23.14% in buffaloes and cows respectively. Mastitis was more prevalent both in buffaloes and cows at the age of 9-10 years ...

The Effects Of Trace Mineral Supplementation On Performance, Health, And Carcass Quality Of At-Risk Mineral Deficient Feedlot Cattle, Tevan J. Brady

All Graduate Theses and Dissertations

Morbidity in feedlot cattle due to disease is a common economical loss for feedlot producers. Utah is not typically considered a ‘feedlot state’, but there are several producers in the southern part of the state that specialize in receiving at-risk cattle. These cattle are at-risk because they are coming from areas known to be mineral deficient. Areas such as the western US, are known to be deficient in several trace minerals important in immune response. Therefore, it is critical that producers have an adequate plan to decrease the negative effects that this has on economic viability of their beef operations ...

Investigation Of Methionine And Lysine Derivatives As A Source Of Rumen-Protected Amino Acids For Lactating Dairy Cows, Mark Avila Fagundes

All Graduate Theses and Dissertations

Cows have a protein requirement for growth, maintenance, and lactation. In order to meet those protein requirements, dairy farmers can supplement or feed cattle with specific amino acids, the building blocks that make-up protein. However, in order for the amino acid product to be effective it must avoid degradation in the rumen and be delivered in the small intestine for absorption. Lysine and methionine have traditionally been recognized as the most limiting amino acids for lactating dairy cows. Therefore, nutrition companies have focused on finding ways to encapsulate or protect lysine and methionine from rumen microbes. The N-acetyl-L-methionine and N-acetyl-L-lysine ...

Jackrabbit Dairy & Food Review, Winter 2020-2021, Dairy And Food Science Department

The Jackrabbit Dairy & Food Review

This is the Winter 2020- 2021 Jackrabbit Dairy and Food Review.

It contains the following articles and information:
Message from the Department Head
New Personnel
Judging Activities
Dairy Club
Food Science Club
Student Accomplishments
Professional Activities of Faculty and Staff
Dairy Facilities
2020 Graduates
Where Are They Now?
Scholarships
Dairy and Food Science Students
Outreach
Research Highlights

Incorporation Of Summer Annual Mixtures Into Grazing Systems In Kentucky, Kelly Marie Mercier

Theses and Dissertations--Plant and Soil Sciences

Utilizing summer annual grass-legume forage mixtures has the potential to improve forage yield and nutritive characteristics, and/or animal performance during times when cool-season pasture growth is limited by high temperatures. Legumes can utilize atmospheric nitrogen, which can increase crude protein and forage digestibility in mixtures. As nitrogen application generally improves both the yield and nutritive characteristics of summer annual forages, but can have a negative effect on legume competitiveness, nitrogen fertilizer recommendations for legume-containing summer annual mixtures are not well established.

Two experiments were conducted to determine the feasibility of utilizing summer annual mixtures in Kentucky, USA. The first ...

Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez

Electronic Theses and Dissertations

This Master thesis evaluates the impact of general health care and eating habits (Study 1), vision care (Study 2), and mental health status (Study 3) on Latino dairy farmworkers lifestyle and work performance in South Dakota. We hypothesized that the health status of dairy workers has a negative impact on the workers’ job performance. In study 1, data from a total of 70 participants were collected, using a face-to-face survey in Spanish, which allowed us to assess various topics and details related to employees’ daily routine tasks, eating habits, and general health status. Furthermore, recommendations to improve general health care ...

Milk Phospholipids Extraction Using Switchable Solvents, Kaavya Rathnakumar

Electronic Theses and Dissertations

Phospholipids (PLs) found in milk are of great interest due to their health and nutritional benefits associated with their consumption. By-products generated during the manufacture of dairy products represent a source of PLs with potential for value-added opportunities. Currently, the extraction of PLs from by-products results in overall lowefficiencies, and it involves subsequent solvent separation and lipid recovery. This dissertation studied the extraction of milk PLs from by-product streams with a tertiary anime (N, N-dimethyl cyclohexylamine, CyNMe2) as a switchable hydrophilicity solvent. This type of solvents can be reversibly switched between a hydrophobic and hydrophilic form in the presence or ...

Gene Network And Pathway Analysis Of Transcriptional Signatures Characterizing Sole Ulcer And Digital Dermatitis In Dairy Cows, Roshin Anie Mohan

Electronic Theses and Dissertations

This study aimed to evaluate the transcriptomic profile of both corium tissue from lactating dairy cows experiencing sole ulcers and skin lesions from lactating dairy cows diagnosed with digital dermatitis (DD). Hoof biopsies were performed in multiparous Holstein dairy cows selected based on their condition as clinically healthy or diagnosed with sole ulcers (n=7/group). Similarly, skin biopsies were taken from the center of active (M2/M4.1) DD lesions or non-active (M0/M4/M1) from multiparous Holstein dairy cows (n = 7/group) to assess the impact of DD on the skin transcriptome via RNA-seq analysis. All RNA samples ...

Nad Metabolism In Male Reproductive Aging, Mirella L. Meyer-Ficca

Funded Research Records

No abstract provided.

Development Of A Grass Measurement Optimisation Tool To Efficiently Measure Herbage Mass On Grazed Pastures, D. J. Murphy, B. O'Brien, Michael D. Murphy

Dept. of Process, Energy & Transport Engineering

Accurate and efficient estimation of herbage mass is essential for optimising grass utilisation and increasing profit for pasture farming. There is no definitive sampling protocol for grass measurement on Irish pastures. This paper presents the Grass Measurement Optimisation Tool (GMOT), designed to generate measurement protocols that optimise for time and accuracy. The GMOT was designed in the form of a decision support tool that generates interactive paddock maps that guide the farmer on how to optimally measure their pastures in a random stratified manner based on GPS co-ordinates, resulting in accurate non-biased estimations of mean herbage mass. Rising plate meter ...

Memantine Treatment Reduces The Incidence Of Flaccid Paralysis In A Zika Virus Mouse Model Of Temporary Paralysis With Similarities To Guillain-Barré Syndrome, Venkatraman Siddharthan, Hong Wang, Alexandre Lr De Oliveria, Xin Dai, John D. Morrey

Animal, Dairy, and Veterinary Science Faculty Publications

Clinical evidence suggests that Zika virus contributes to Guillain-Barré syndrome that causes temporary paralysis. We utilized a recently described Zika virus mouse model of temporary flaccid paralysis to address the hypothesis that treatment with an N-methyl-D-aspartate receptor antagonist, memantine, can reduce the incidence of paralysis. Aged interferon alpha/beta-receptor knockout mice were used because of their sublethal susceptibility to Zika virus infection. Fifteen to twenty-five percent of mice infected with a Puerto Rico strain of Zika virus develop acute flaccid paralysis beginning at days 8–9 and peaked at days 10–12. Mice recover from paralysis within a week ...

Photovoltaic Systems On Dairy Farms: Financial And Renewable Multi-Objective Optimization (Farmoo) Analysis, Michael Breen, J. Upton, Michael D. Murphy

Dept. of Process, Energy & Transport Engineering

No abstract provided.

Association Between Single Nucleotide Polymorphisms In Sirt1 And Sirt2 Loci And Growth In Tibetan Sheep, Lin-Sheng Gui, Sayed Haidar Abbas Raza, Li Zhou, Matthew Garcia, Ayman Hassan Abd El-Aziz, Dawei Wei, Shengzhen Hou, Jianlei Jia, Zhiyou Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Silent information regulator 1 and 2 (SIRT1, 2) were NAD+-dependent histone or non-histone deacetylase, which emerged as key metabolic sensors in several tissues of mammals. In the present study, the search for polymorphisms within the ovine SIRT1 and SIRT2 loci as well as association analyses between SNPs and growth-related traits were performed in Tibetan sheep. To determine the expression pattern of SIRT1 and SIRT2 genes in Tibetan sheep, the quantitative real-time polymerase chain reaction (qPCR) analysis revealed that those two genes were widely expressed in diverse tissues. Expression of SIRT1 was less in abomasum of lamb, whereas it was ...

The Effects Of Different Organic Pastures On Dairy Heifer Growth And Development, Jacob A. Hadfield

All Graduate Theses and Dissertations

Raising dairy heifers in a certified organic setting can be difficult for producers. Conventionally, heifers are raised in a confined setting, and fed a total mixed ration (TMR) that is balanced daily to contain all the needed nutrients for developing heifers. Organic producers can use a TMR in their operations, but due to high organic feed costs, many choose to raise their heifers in pasture-based systems. While pasture-based systems may lower costs, heifers on pasture commonly have lower rates of gain, which can be financially burdensome to producers. Grass-legume pastures may help improve rates of gain in heifers on pasture-based ...

Characterization Of A Novel Stat 2 Knock Out Hamster Model Of Crimean Congo Hemorrhagic Fever Virus Pathogenesis, Charlene Ranadheera, Emelissa J. Valcourt, Bryce M. Warner, Guillaume Poliquin, Kyle Rosenke, Kathy Frost, Kevin Tierney, Greg Saturday, Jinxin Miao, Jonna B. Westover, Brian B. Gowen, Stephanie Booth, Heinz Feldmann, Zhongde Wang, David Safronetz

Animal, Dairy, and Veterinary Science Faculty Publications

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne pathogen causing a febrile illness in humans, which can progress to hemorrhagic manifestations, multi-organ failure, and death. Current mouse models of CCHFV infection reliably succumb to virus challenge but vary in their ability to reflect signs of disease similar to humans. In this study, we established a signal transducer and activator of transcription 2 (STAT2) knockout hamster model to expand the repertoire of animal models of CCHFV pathogenesis that can be used for therapeutic development. These hamsters demonstrated a systemic and lethal disease in response to infection. Hallmarks of human disease ...

The Use Of The Heter-Lp Algorithm To Reposition Antibiotics For Managing E. Coli Mastitis In Dairy Cattle, Somayeh Sharifi, Maryam Lotfi Shahreza, Abbas Pakdel, James M. Reecy, Nasser Ghadiri, Hadi Atashi, Mahmoud Motamedi, Esmaeil Ebrahimie

Animal Science Publications

Mastitis, a disease with high incidence worldwide, is the most prevalent and costly disease in the dairy industry. Environmental mastitis pathogens, such as Escherichia coli (E. coli), are major etiological agents of bovine mastitis in well-managed dairy farms. However, there is still a need to develop more efficient, safe, and economical treatments for mastitis. In the current research, Heter-LP, a new system biology-based method of drug repositioning, was applied to potentially identify novel therapeutic avenues for the treatment of E. coli mastitis. On-line data repositories relevant to known diseases, drugs, and gene targets along with other specialized biological information for ...

The Effect Of Dairy Cattle Housing Systems On The Concentrations And Emissions Of Gaseous Mixtures In Barns Determined By Fourier-Transform Infrared Spectroscopy, Dorota Witkowska, Mariusz Korczyński, Jacek A. Koziel, Janina Sowińska, Bartosz Chojnowski

Agricultural and Biosystems Engineering Publications

The aim of this study was to determine the concentrations and emissions of greenhouse and odorous gases in different types of dairy cattle housing systems with the use of Fourier-transform infrared (FTIR) spectroscopy. The study was performed in autumn and winter in four types of dairy cattle barns with different process and technical systems (free-stall, deep litter – FS-DL; free-stall, sub-floor manure storage – FS-SFM; free-stall, litter in stalls – FS-LS; tie-stall, litter in stalls – TS-LS) in northern Poland. Analyses of gaseous mixtures in barn air were conducted by infrared spectrometry with the multi-component Gasmet DX4030 analyzer. A total of 200 measurement spectra ...

Raman Spectroscopy Characterization Extracellular Vesicles From Bovine Placenta And Peripheral Blood Mononuclear Cells, Han Zhang, Ana Caroline Silva, Wei Zhang, Heloisa M. Rutigliano, Anhong Zhou

Animal, Dairy, and Veterinary Science Faculty Publications

Placenta-derived extracellular vesicles (EVs) are involved in communication between the placenta and maternal immune cells possibly leading to a modulation of maternal T-cell signaling components. The ability to identify EVs in maternal blood may lead to the development of diagnostic and treatment tools for pregnancy complications. The objective of this work was to differentiate EVs from bovine placenta (trophoblast) and peripheral blood mononuclear cells (PBMC) by a label-free, non-invasive Raman spectroscopy technique. Extracellular vesicles were isolated by ultracentrifugation. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) were applied to verify the presence and the size distribution of EVs. Raman ...

Comparison Of In Vitro Inactivation Of Sars Cov-2 With Hydrogen Peroxide And Povidone-Iodine Oral Antiseptic Rinses, Avinash S. Bidra, Jesse S. Pelletier, Jonna B. Westover, Samantha Frank, Seth M. Brown, Belachew Tessema

Animal, Dairy, and Veterinary Science Faculty Publications

Purpose

To evaluate the in vitro inactivation of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) with hydrogen peroxide (H2O2) and povidone‐iodine (PVP‐I) oral antiseptic rinses at clinically recommended concentrations and contact times.

Materials and Methods

SARS‐CoV‐2, USA‐WA1/2020 strain virus stock was prepared prior to testing by growing in Vero 76 cells. The culture media for prepared virus stock was minimum essential medium (MEM) with 2% fetal bovine serum (FBS) and 50 µg/mL gentamicin. Test compounds consisting of PVP‐I oral rinse solutions and H2O2 aqueous solutions were mixed directly with the ...

Rapid In-Vitro Inactivation Of Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-Cov-2) Using Povidone-Iodine Oral Antiseptic Rinse, Avinash S. Bidra, Jesse S. Pelletier, Jonna B. Westover, Samantha Frank, Seth M. Brown, Belachew Tessema

Animal, Dairy, and Veterinary Science Faculty Publications

Purpose

To investigate the optimal contact time and concentration for viricidal activity of oral preparation of povidone‐iodine (PVP‐I) against SARS‐CoV‐2 (‘corona virus’) to mitigate the risk and transmission of the virus in the dental practice.

Materials and Methods

The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) USA‐WA1/2020 strain, virus stock was tested against oral antiseptic solutions consisting of aqueous povidone‐iodine (PVP‐I) as the sole active ingredient. The PVP‐I was tested at diluted concentrations of 0.5%, 1%, and 1.5%. Test media without any virus was added to 2 ...