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Coitus-Free Sexual Transmission Of Zika Virus In A Mouse Model, Chad S. Clancy, Arnaud J. Van Wettere, John D. Morrey, Justin G. Julander 2018 Utah State University

Coitus-Free Sexual Transmission Of Zika Virus In A Mouse Model, Chad S. Clancy, Arnaud J. Van Wettere, John D. Morrey, Justin G. Julander

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) is an arboviral infection that may be sexually transmitted. The present study aims to determine if accessory sex glands are a potential source of infectious virus and important in sexual transmission. Male interferon type I receptor knockout (Ifnar−/−) mice were challenged subcutaneously with a Puerto Rican ZIKV isolate. Reproductive tissues were harvested seven days after viral challenge and artificial insemination fluid derived from epididymis or homogenized accessory sex glands (seminal plasma) was obtained. Naïve interferon type I and II receptor knockout (AG129) females were pretreated with progesterone, and inoculated intravaginally with either epididymal flush or seminal plasma ...


James Roger Thomas, B. 1950 And Doris (Carson) Thomas (Sc 3273), Manuscripts & Folklife Archives 2018 Western Kentucky University

James Roger Thomas, B. 1950 And Doris (Carson) Thomas (Sc 3273), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 3273. Speech of Roger and Doris Thomas, Smiths Grove, Kentucky, given at a dairymen's event. They describe the history and operation of their dairy farm and their family and community activities. The remarks were made in conjunction with their forthcoming participation in a competition as representatives of the group.


Intramammary Infusion Of Casein Hydrolysate For Involution Of Single Mastitic Mammary Quarters Elevating Cow-Level Somatic Cell Count, Justine Britten, Kerry A. Rood, David J. Wilson 2018 Utah State University

Intramammary Infusion Of Casein Hydrolysate For Involution Of Single Mastitic Mammary Quarters Elevating Cow-Level Somatic Cell Count, Justine Britten, Kerry A. Rood, David J. Wilson

Animal, Dairy, and Veterinary Science Faculty Publications

Mastitis in a single quarter can cause high somatic cell counts (SCC), clinical mastitis, and death in dairy cows. Currently, management of these mastitic quarters presents a problem for the dairy industry. Casein hydrolysate (CH) is an intramammary (IMM) infusion treatment reported to induce mammary involution. The primary objectives of this study were to investigate whether IMM CH treatment of single high SCC quarters, followed by cessation of quarter milk production for the remainder of lactation, was effective in reducing cow–level SCC and whether that quarter resumed milk production following calving. Three treatment groups were used: CH, non-hydrolyzed casein ...


Early Events In Japanese Encephalitis Virus Infection: Viral Entry, Sang-Im Yun, Young-Min Lee 2018 Utah State University

Early Events In Japanese Encephalitis Virus Infection: Viral Entry, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), a mosquito-borne zoonotic flavivirus, is an enveloped positive-strand RNA virus that can cause a spectrum of clinical manifestations, ranging from mild febrile illness to severe neuroinvasive disease. Today, several killed and live vaccines are available in different parts of the globe for use in humans to prevent JEV-induced diseases, yet no antivirals are available to treat JEV-associated diseases. Despite the progress made in vaccine research and development, JEV is still a major public health problem in southern, eastern, and southeastern Asia, as well as northern Oceania, with the potential to become an emerging global pathogen. In ...


Functional Genomics And Immunologic Tools: The Impact Of Viral And Host Genetic Variations On The Outcome Of Zika Virus Infection, Sang-Im Yun, Byung-Hak Song, Jordan C. Frank, Justin G. Julander, Aaron L. Olsen, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Young-Min Lee 2018 Utah State University

Functional Genomics And Immunologic Tools: The Impact Of Viral And Host Genetic Variations On The Outcome Of Zika Virus Infection, Sang-Im Yun, Byung-Hak Song, Jordan C. Frank, Justin G. Julander, Aaron L. Olsen, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) causes no-to-mild symptoms or severe neurological disorders. To investigate the importance of viral and host genetic variations in determining ZIKV infection outcomes, we created three full-length infectious cDNA clones as bacterial artificial chromosomes for each of three spatiotemporally distinct and genetically divergent ZIKVs: MR-766 (Uganda, 1947), P6-740 (Malaysia, 1966), and PRVABC-59 (Puerto Rico, 2015). Using the three molecularly cloned ZIKVs, together with 13 ZIKV region-specific polyclonal antibodies covering nearly the entire viral protein-coding region, we made three conceptual advances: (i) We created a comprehensive genome-wide portrait of ZIKV gene products and their related species, with several previously ...


Does Stat5a Have An Effect On Bmal1 Levels In Mammary Epithelial Cells?, Clare E. Aduwari, Aridany Suarez-Trujillo, Karen I. Plaut, Theresa M. Casey 2018 Purdue University

Does Stat5a Have An Effect On Bmal1 Levels In Mammary Epithelial Cells?, Clare E. Aduwari, Aridany Suarez-Trujillo, Karen I. Plaut, Theresa M. Casey

The Summer Undergraduate Research Fellowship (SURF) Symposium

The mammary gland is a very important organ for reproduction in mammals because it produces milk which serves as the primary source of nutrients for newly-born offspring. Previous studies suggest that its development is regulated by circadian clocks, biochemical oscillators that generate circadian rhythms (the body’s internal clock). The circadian system plays a major role in homeostasis, coordinating the body’s internal physiology and synchronizing it with the external environment. Our lab showed that levels of the BMAL1 protein, a core clock component, increased in the mammary gland at the onset of lactation. Treatment of mammary epithelial cells (HC11 ...


Immunogenicity And Protection After Vaccination With A Modified Vaccinia Virus Ankara-Vectored Yellow Fever Vaccine In The Hamster Model, Justin G. Julander, Marco Testori, Cédric Cheminay, Ariane Volkmann 2018 Utah State University

Immunogenicity And Protection After Vaccination With A Modified Vaccinia Virus Ankara-Vectored Yellow Fever Vaccine In The Hamster Model, Justin G. Julander, Marco Testori, Cédric Cheminay, Ariane Volkmann

Animal, Dairy, and Veterinary Science Faculty Publications

The highly efficacious live-attenuated 17D yellow fever (YF) vaccine is occasionally associated with rare life-threatening adverse events. Modified vaccinia virus Ankara (MVA), a non-replicating poxvirus, has been used as a vaccine platform to safely deliver various antigens. A MVA-based YF vaccine (MVA-BN-YF) was tested with and without a non-mineral oil adjuvant in a hamster model of lethal YF disease and protective efficacy of this vaccine was compared with the 17D vaccine. The vaccine candidate MVA-BN-YF generated a protective response in hamsters infected with YFV that was comparable to protection by the live 17D vaccine. Similar levels of neutralizing antibody were ...


A Non-Recoverable Hybridoma Limits The Production Of Monoclonal Antibodies Against Bovine Trophoblast Non-Classical Nc3*00101 Protein, Parveen Parasar, Amanda Wilhelm, William C. Davis, Kenneth L. White, Christopher J. Davies 2018 Utah State University

A Non-Recoverable Hybridoma Limits The Production Of Monoclonal Antibodies Against Bovine Trophoblast Non-Classical Nc3*00101 Protein, Parveen Parasar, Amanda Wilhelm, William C. Davis, Kenneth L. White, Christopher J. Davies

Animal, Dairy, and Veterinary Science Faculty Publications

The non-classical MHC class I (MHC-Ib) proteins are important modulators of immune system during pregnancy favoring survival of the fetus. Contrary to ubiquitously expressed classical MHC-I proteins, MHC-Ib proteins are oligomorphic, and expressed in specific cell/tissue types thus minimizing maternal immune-mediated rejection of fetal-allograft and a successful pregnancy. A unique characteristic of MHC-Ib glycoproteins is expression of surface and soluble isoforms due to alternative splicing phenomenon. Bovine fetal trophoblast cells, during the third trimester of pregnancy, express non-classical bovine leukocyte antigen class Ib (BoLA-Ib) antigens. BoLA-NC3*00101, is a non-classical class I allele from cattle AH11 haplotype and is ...


Phenotypic Association Between Lactose And Other Milk Components In Western Us Dairy Herds And Japan, Takuji Asami 2018 Utah State University

Phenotypic Association Between Lactose And Other Milk Components In Western Us Dairy Herds And Japan, Takuji Asami

All Graduate Theses and Dissertations

Lactose in milk has relatively low variation regardless of season, breed, or country. The study of lactose concentration and correlation among other milk components is limited. Furthermore, dairy farmers have limited access to the lactose data and are not familiar with it. This study was conducted to: 1) investigate the phenotypic correlation between lactose and other milk components; and 2) determine the importance of lactose for dairy herds.

Monthly DHIA records from Utah (DHIA), Dairy Herd Performance Test (DHTP) records from Ibaraki, Japan, and California herd average data (CHAD) covering 27 states were used to analyze the relationships between milk ...


Why Forage Quality Matters, John Olthoff 2018 Dordt College

Why Forage Quality Matters, John Olthoff

Faculty Work Comprehensive List

No abstract provided.


Urine Volume And Nitrogen Excretion Are Altered By Feeding Birdsfoot Trefoil Compared With Alfalfa In Lactating Dairy Cows, Mohammad Ghelichkhan, Jong-Su Eun, Rachael G. Christensen, Rusty D. Stott, Jennifer W. MacAdam 2018 Utah State University

Urine Volume And Nitrogen Excretion Are Altered By Feeding Birdsfoot Trefoil Compared With Alfalfa In Lactating Dairy Cows, Mohammad Ghelichkhan, Jong-Su Eun, Rachael G. Christensen, Rusty D. Stott, Jennifer W. Macadam

Animal, Dairy, and Veterinary Science Faculty Publications

Legumes that contain condensed tannins may have lower ruminal protein degradation than alfalfa. The present study investigated the effects of feeding birdsfoot trefoil (Lotus corniculatus L.) hay on lactational performance and N utilization and excretion. Eight multiparous Holstein cows in midlactation (150 ± 22.3 d-in-milk) were randomly assigned to 2 treatments [alfalfa hay-based total mixed ration (AHT) or birdsfoot trefoil hay-based total mixed ration (BHT)] in a crossover design with 2 experimental periods. Each experimental period lasted 17 d (14 d of adaptation and 3 d of sampling and total collection). Hays comprised approximately 50% of DM in experimental diets ...


Consequences Of In Utero Exposure To Zika Virus In Offspring Of Ag129 Mice, Justin G. Julander, Venkatraman Siddharthan, Albert H. Park, Elizabeth S. Preston, Pranav Mathur, Michael Bertolio, Hong Wang, Katherine Zukor, Arnaud J. Van Wettere, Donal G. Sinex, John D. Morrey 2018 Utah State University

Consequences Of In Utero Exposure To Zika Virus In Offspring Of Ag129 Mice, Justin G. Julander, Venkatraman Siddharthan, Albert H. Park, Elizabeth S. Preston, Pranav Mathur, Michael Bertolio, Hong Wang, Katherine Zukor, Arnaud J. Van Wettere, Donal G. Sinex, John D. Morrey

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) can cause various diseases in offspring after congenital infection. The purpose of this study was to identify disease phenotypes in pups exposed to ZIKV in utero. Female interferon-α/β, -γ receptor knockout mice (AG129) were infected intraperitoneally with ZIKV 7.5 days’ post coitus (dpc). Viral RNA, antigen and infectious virus were detected in some, but not all, maternal and fetal tissues at various times during gestation. Fetuses of infected dams had significant intrauterine growth restriction (IUGR), which was more pronounced as females neared parturition. Pups born to infected dams were significantly smaller and had significantly shortened ...


Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender 2018 Fordham University

Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender

Student Theses 2015-Present

This paper aims to shed light on the dissonance caused by the superimposition of Dominant Human Systems on Natural Systems. I highlight the synthetic nature of Dominant Human Systems as egoic and linguistic phenomenon manufactured by a mere portion of the human population, which renders them inherently oppressive unto peoples and landscapes whose wisdom were barred from the design process. In pursuing a radical pragmatic approach to mending the simultaneous oppression and destruction of the human being and the earth, I highlight the necessity of minimizing entropic chaos caused by excess energy expenditure, an essential feature of systems that aim ...


What’S At Steak? The Political Discourse Of Emissions Intensity And Implications Of Animal Agriculture, Kalyn Simon 2018 The University of San Francisco

What’S At Steak? The Political Discourse Of Emissions Intensity And Implications Of Animal Agriculture, Kalyn Simon

Master's Theses

This thesis analyzes the international discourse of greenhouse gas emissions in relation to livestock production. Specific government ministries, research institutes, and multilateral organizations are framing a new strategy to mitigate emissions by using emissions intensity metrics. Emissions intensity is a ratio comparing emissions produced per unit of animal product as oppose to measuring absolute emissions. This research is acknowledged as a win-win scenario which allows for a reduction of greenhouse gas emissions while continuing production more efficiently. This thesis outlines the emergence of this metric in international discourse and the implications this shift has on the livestock sector globally. Ultimately ...


Characterization Of An N-Terminal Non-Core Domain Of Rag1 Gene Disrupted Syrian Hamster Model Generated By Crispr Cas9, Jinxin Miao, Baoling Ying, Rong Li, Ann E. Tollefson, Jacqueline F. Spencer, William S.M. Wold, Seok-Hwan Song, Il-Keun Kong, Karoly Toth, Yaohe Wang, Zhongde Wang 2018 Zhengzhou University

Characterization Of An N-Terminal Non-Core Domain Of Rag1 Gene Disrupted Syrian Hamster Model Generated By Crispr Cas9, Jinxin Miao, Baoling Ying, Rong Li, Ann E. Tollefson, Jacqueline F. Spencer, William S.M. Wold, Seok-Hwan Song, Il-Keun Kong, Karoly Toth, Yaohe Wang, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

The accumulating evidence demonstrates that Syrian hamsters have advantages as models for various diseases. To develop a Syrian hamster (Mesocricetus auratus) model of human immunodeficiency caused by RAG1 gene mutations, we employed the CRISPR/Cas9 system and introduced an 86-nucleotide frameshift deletion in the hamster RAG1 gene encoding part of the N-terminal non-core domain of RAG1. Histological and immunohistochemical analyses demonstrated that these hamsters (referred herein as RAG1-86nt hamsters) had atrophic spleen and thymus, and developed significantly less white pulp and were almost completely devoid of splenic lymphoid follicles. The RAG1-nt86 hamsters had barely detectable CD3+ and CD4+ T cells ...


Using Indirect Calorimetry To Investigate Feeding Value Of Byproducts For Lactating Dairy Cattle: Canola Meal And Dried Distillers Grains And Solubles, Mickayla A. Myers 2018 University of Nebraska - Lincoln

Using Indirect Calorimetry To Investigate Feeding Value Of Byproducts For Lactating Dairy Cattle: Canola Meal And Dried Distillers Grains And Solubles, Mickayla A. Myers

Theses and Dissertations in Animal Science

Byproducts have played a major role in dairy nutrition by providing a cheaper protein and fiber source, while also utilizing a product that would otherwise be waste from the human perspective. Use of byproducts in the dairy industry should allow for continued and overall increases in production and efficiency of the dairy industry. Two of the more popular byproducts in the dairy industry today are dried distillers grains and solubles and canola meal.

In the first experiment, 12 multiparous lactating Jersey cows were used evaluate the feeding value of dried distillers grains and solubles (DDGS) or canola meal. A replicated ...


Evaluation Of Single Vs. Pair Housing Holstein Calves On Specific Antibody Concentrations To Klh, Chaney K. Morgan 2018 University of Tennessee, Knoxville

Evaluation Of Single Vs. Pair Housing Holstein Calves On Specific Antibody Concentrations To Klh, Chaney K. Morgan

University of Tennessee Honors Thesis Projects

No abstract provided.


Bovine Mastitis Resistance: Novel Quantitative Trait Loci And The Role Of Bovine Mammary Epithelial Cells, Jacqueline P. Kurz 2018 Utah State University

Bovine Mastitis Resistance: Novel Quantitative Trait Loci And The Role Of Bovine Mammary Epithelial Cells, Jacqueline P. Kurz

All Graduate Theses and Dissertations

Bovine mastitis, or inflammation of the mammary gland, has substantial economic and animal welfare implications. A genetic basis for mastitis resistance traits is recognized and can be used to guide selective breeding programs. The discovery of regions of the genome associated with mastitis resistance, and knowledge of the underlying molecular mechanisms responsible, can facilitate development of efficient mastitis control and therapeutic strategies. The objectives of this dissertation research were to identify sites of genetic variation associated with mastitis resistance, and to define the contributions of the milk-secreting epithelial cells to mammary gland immune responses and mastitis resistance. Twenty seven regions ...


Effect Of Nightly Mixing Versus Separation Of Dams And Calves On Behavior, Production, And Calf Growth, Ashley Danielle Campeaux 2018 University of Tennessee, Knoxville

Effect Of Nightly Mixing Versus Separation Of Dams And Calves On Behavior, Production, And Calf Growth, Ashley Danielle Campeaux

University of Tennessee Honors Thesis Projects

No abstract provided.


In-Vitro Digestibility And Gas Production Of Wheat Middlings, Solvent Extracted Cottonseed Meal, Soyhulls, And Corn Gluten Feed And The Effects Of Monensin On In-Vitro Digestibility And Gas Production, Dustin W. DeVore 2018 Missouri State University

In-Vitro Digestibility And Gas Production Of Wheat Middlings, Solvent Extracted Cottonseed Meal, Soyhulls, And Corn Gluten Feed And The Effects Of Monensin On In-Vitro Digestibility And Gas Production, Dustin W. Devore

MSU Graduate Theses

This study was designed to study the in-vitro digestibility and in-vitro total gas production of corn gluten feed, wheat middlings, solvent extracted cottonseed meal, and soyhulls in multi parous steers. Evaluation of the effects of monensin on in-vitro digestibility and in-vitro total overall gas production of a commercially available complete feed was also studied Feedstuffs were ground through a Wiley mill to ensure uniform samples and were added to digestion jars in a completely randomized design. Rumen fluid was collected from a Bos taurus steer via a ruminal cannula. To ensure consistent digesta material the steer was fed a uniform ...


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