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Evaluation Of Casein Hydrolysate As An Alternative Dry-Off Treatment And Milk Quality Management Tool In Dairy Cows, Justine Elena Britten 2019 Utah State University

Evaluation Of Casein Hydrolysate As An Alternative Dry-Off Treatment And Milk Quality Management Tool In Dairy Cows, Justine Elena Britten

All Graduate Theses and Dissertations

Mastitis, an infection of the mammary gland, is the most common and expensive animal health problem for the dairy industry and affects every dairy farm to some degree. This disease complex is painful for dairy cows, increases the on-farm use of antibiotics, presents a threat to milk quality and is a waste of time, money and milk production. Each year, the dairy industry loses as much as a billion dollars to mastitis.

Many cows will experience mastitis at least once during a lactation cycle and some animals will develop recurring mastitis episodes in a single mammary quarter. These mastitic quarters ...


Ruminal Protection And Intestinal Availability Of Rumen-Protected Methionine And Lysine In Lactating Dairy Cows, Sara Menchu 2019 Utah State University

Ruminal Protection And Intestinal Availability Of Rumen-Protected Methionine And Lysine In Lactating Dairy Cows, Sara Menchu

All Graduate Theses and Dissertations

Rumen protected Methionine (MET) and Lysine (LYS) are critical for milk protein synthesis in dairy cows. N-acetyl-L-methionine (NALM) is a MET derivative that consists of L-Met protected with an acetyl group that is attached to the α-amino group.N-acetyl-L-lysine (NALL) is a LYS derivative that is similarly protected. The objectives of these studies were to quantify the gastrointestinal availability of NALM and NALL. Three experiments were run as 3 × 3 Latin square using 3 second lactation Holstein cows that have been fitted with cannulas in the rumen and duodenum. The cows were fed diets containing the supplements for two weeks ...


Observing The Effects Of Aspergillus Oryzae On Combating The Consequences Of Heat Stress In Lactating Dairy Cows, Abigail Lorraine Kesterson 2019 University of Tennessee, Knoxville

Observing The Effects Of Aspergillus Oryzae On Combating The Consequences Of Heat Stress In Lactating Dairy Cows, Abigail Lorraine Kesterson

Chancellor’s Honors Program Projects

No abstract provided.


The Role Of T-Cell Lymphoma Invasion And Metastasis 2 (Tiam2) In The Barrier Function Of Mammary Epithelial Cells Following Streptococcus Uberis Inoculation, Catherine E. Donnell 2019 The University of Tennessee Knoxville

The Role Of T-Cell Lymphoma Invasion And Metastasis 2 (Tiam2) In The Barrier Function Of Mammary Epithelial Cells Following Streptococcus Uberis Inoculation, Catherine E. Donnell

Chancellor’s Honors Program Projects

No abstract provided.


Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden 2019 University of Tennessee Health Science Center

Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden

Justin G. Julander

Currently, there are no licensed human vaccines or antivirals for treatment of or prevention from infection with encephalitic alphaviruses. Because epidemics are sporadic and unpredictable, and endemic disease is common but rarely diagnosed, it is difficult to identify all populations requiring vaccination; thus, an effective post-exposure treatment method is needed to interrupt ongoing outbreaks. To address this public health need, we have continued development of ML336 to deliver a molecule with prophylactic and therapeutic potential that could be relevant for use in natural epidemics or deliberate release scenario for Venezuelan equine encephalitis virus (VEEV). We report findings from in vitro ...


Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe 2019 University of Minnesota

Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe

Roger A. Coulombe, Jr.

The nearly-ubiquitous food and feed-borne mycotoxin aflatoxin B1 (AFB1) is carcinogenic and mutagenic, posing a food safety threat to humans and animals. One of the most susceptible animal species known and thus a good model for characterizing toxicological pathways, is the domesticated turkey (DT), a condition likely due, at least in part, to deficient hepatic AFB1-detoxifying alpha-class glutathione S-transferases (GSTAs). Conversely, wild turkeys (Eastern wild, EW) are relatively resistant to the hepatotoxic, hepatocarcinogenic and immunosuppressive effects of AFB1 owing to functional gene expression and presence of functional hepatic GSTAs. This study was designed to compare ...


Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang 2019 Utah State University

Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang

Zhongde Wang

Zika virus (ZIKV) infection can cause severe congenital diseases, such as microcephaly, ocular defects and arthrogryposis in fetuses, and Guillain–Barré syndrome in adults. Efficacious therapeutic treatments for infected patients, as well as prophylactic treatments to prevent new infections are needed for combating ZIKV infection. Here, we report that ZIKV-specific human polyclonal antibodies (SAB-155), elicited in transchromosomal bovine (TcB), provide significant protection from infection by ZIKV in STAT2 knockout (KO) golden Syrian hamsters both prophylactically and therapeutically. These antibodies also prevent testicular lesions in this hamster model. Our data indicate that antibody-mediated immunotherapy is effective in treating ZIKV infection. Because ...


Standardization And Extrapolation Of Icing Stability, Shelf Life, And Safety Under Heat Abuse, Jaden Castinado 2019 Kansas State University Libraries

Standardization And Extrapolation Of Icing Stability, Shelf Life, And Safety Under Heat Abuse, Jaden Castinado

Kansas State University Undergraduate Research Conference

Inquiries from Kansas citizens requested information on the stability, shelf life, and safety of icings on baked goods after prolonged exposure to heat without refrigeration, often common at county and state fairs. Methods include compiling a sample of icing recipes from Kansas citizens and entering into a database so that recipes could be sorted by factors such as sugar and/or dairy content. A central composite design will then be used to identify trials with variable amounts of sugar, dairy, and sugar with dairy. Current work is focused on creating a concise and navigable database with sufficient data to create ...


Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden 2019 University of Tennessee Health Science Center

Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden

Animal, Dairy, and Veterinary Science Faculty Publications

Currently, there are no licensed human vaccines or antivirals for treatment of or prevention from infection with encephalitic alphaviruses. Because epidemics are sporadic and unpredictable, and endemic disease is common but rarely diagnosed, it is difficult to identify all populations requiring vaccination; thus, an effective post-exposure treatment method is needed to interrupt ongoing outbreaks. To address this public health need, we have continued development of ML336 to deliver a molecule with prophylactic and therapeutic potential that could be relevant for use in natural epidemics or deliberate release scenario for Venezuelan equine encephalitis virus (VEEV). We report findings from in vitro ...


Fermentation Of Prebiotics In Whole Food Powders By Probiotic Bacteria Strains, Michaela Brubaker, Sumira Phatak 2019 Utah State University

Fermentation Of Prebiotics In Whole Food Powders By Probiotic Bacteria Strains, Michaela Brubaker, Sumira Phatak

Research on Capitol Hill

Colorectal cancer (CRC) is the second leading cause of cancer death in the United States. Modification of the gut microbiome offers potential for CRC prevention.


Generation Of H7n9-Specific Human Polyclonal Antibodies From A Transchromosomic Goat (Caprine) System, Hua Wu, Zhiqiang Fan, Michelle Brandsrud, Qinggang Meng, Molly Bobbitt, Misha Regouski, Rusty Stott, Alexis Sweat, Jackelyn Crabtree, Robert J. Hogan, Ralph A. Tripp, Zhongde Wang, Irina A. Polejaeva, Eddie J. Sullivan 2019 SAB Biotherapeutics

Generation Of H7n9-Specific Human Polyclonal Antibodies From A Transchromosomic Goat (Caprine) System, Hua Wu, Zhiqiang Fan, Michelle Brandsrud, Qinggang Meng, Molly Bobbitt, Misha Regouski, Rusty Stott, Alexis Sweat, Jackelyn Crabtree, Robert J. Hogan, Ralph A. Tripp, Zhongde Wang, Irina A. Polejaeva, Eddie J. Sullivan

Animal, Dairy, and Veterinary Science Faculty Publications

To address the unmet needs for human polyclonal antibodies both as therapeutics and diagnostic reagents, building upon our previously established transchromosomic (Tc) cattle platform, we report herein the development of a Tc goat system expressing human polyclonal antibodies in their sera. In the Tc goat system, a human artificial chromosome (HAC) comprising the entire human immunoglobulin (Ig) gene repertoire in the germline configuration was introduced into the genetic makeup of the domestic goat. We achieved this by transferring the HAC into goat fetal fibroblast cells followed by somatic cell nuclear transfer for Tc goat production. Gene and protein expression analyses ...


Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang 2019 Utah State University

Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection can cause severe congenital diseases, such as microcephaly, ocular defects and arthrogryposis in fetuses, and Guillain–Barré syndrome in adults. Efficacious therapeutic treatments for infected patients, as well as prophylactic treatments to prevent new infections are needed for combating ZIKV infection. Here, we report that ZIKV-specific human polyclonal antibodies (SAB-155), elicited in transchromosomal bovine (TcB), provide significant protection from infection by ZIKV in STAT2 knockout (KO) golden Syrian hamsters both prophylactically and therapeutically. These antibodies also prevent testicular lesions in this hamster model. Our data indicate that antibody-mediated immunotherapy is effective in treating ZIKV infection. Because ...


Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe 2019 University of Minnesota

Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe

Animal, Dairy, and Veterinary Science Faculty Publications

The nearly-ubiquitous food and feed-borne mycotoxin aflatoxin B1 (AFB1) is carcinogenic and mutagenic, posing a food safety threat to humans and animals. One of the most susceptible animal species known and thus a good model for characterizing toxicological pathways, is the domesticated turkey (DT), a condition likely due, at least in part, to deficient hepatic AFB1-detoxifying alpha-class glutathione S-transferases (GSTAs). Conversely, wild turkeys (Eastern wild, EW) are relatively resistant to the hepatotoxic, hepatocarcinogenic and immunosuppressive effects of AFB1 owing to functional gene expression and presence of functional hepatic GSTAs. This study was designed to compare ...


Jackrabbit Dairy And Food Review, Vikram V. Mistry 2019 South Dakota State University

Jackrabbit Dairy And Food Review, Vikram V. Mistry

The Jackrabbit Dairy & Food Review

This is the Winter 2017- 2018 Jackrabbit Dairy and Food Review. It contains the following articles and information: Message from the Department Head, 2017 Graduates, David A. Thompson Endowment, New Faculty and Visitors, information on the Dairy Club and Food Science Club, Judging Activities, Student Accomplishments, Professional Activities of Faculty and Staff, Distinguished Alumni, Dairy Facilities, a list of Scholarships and Dairy and Food Science Students, information on Departmental Outreach and Research Highlights.


Validation Of An Automated Behavior Monitoring Collar, And Evaluation Of Heat Stress On Lactating Dairy Cow Behavior With Access To A Free Choice Soaker, Lori Nicole Grinter 2019 University of Kentucky

Validation Of An Automated Behavior Monitoring Collar, And Evaluation Of Heat Stress On Lactating Dairy Cow Behavior With Access To A Free Choice Soaker, Lori Nicole Grinter

Theses and Dissertations--Animal and Food Sciences

Precision dairy technologies (PDT) are becoming more accessible and are therefore becoming more common on commercial dairy farms and in dairy research. Prior to any use of PDT, one should understand the precision, accuracy and bias of the device by a validations studies before interpreting the behavior measurements. Thus, the objective of the first section of my thesis is to validate ruminating, feeding and resting measurements of a behavior monitoring collar used in the second section. Precision dairy technology is used in heat stress studies to compare behavior of cows exposed to different heat stress treatments or abatement strategies. Heat ...


Automated Body Condition Scoring: Progression Across Lactation And Its Association With Disease And Reproduction In Dairy Cattle, Carissa Marie Truman 2019 University of Kentucky

Automated Body Condition Scoring: Progression Across Lactation And Its Association With Disease And Reproduction In Dairy Cattle, Carissa Marie Truman

Theses and Dissertations--Animal and Food Sciences

Body condition scoring is a technique used to noninvasively assess fat reserves. It provides an objective estimate to describe the current and past nutritional status of the dairy cow and has been associated with increased disease risk and breeding success. Traditionally body condition scores are taken manually by visual appraisal on a 1 to 5 scale, in one-quarter increments. However, recent studies have shown the potential of automating the body condition scoring of cows using images. The first objective was to estimate the likelihood of disease development and breeding success, using odds ratios, associated with body condition score scored automatically ...


Evaluation Of Effects Of Methods Used For Forage Analysis And Dietary Inclusion Of Buffer On Fiber Digestibility And Rumen Fermentation In Diets High In Distillers Grains, Lydia Kehinde Olagunju 2019 South Dakota State University

Evaluation Of Effects Of Methods Used For Forage Analysis And Dietary Inclusion Of Buffer On Fiber Digestibility And Rumen Fermentation In Diets High In Distillers Grains, Lydia Kehinde Olagunju

Electronic Theses and Dissertations

Forage quality is an important factor affecting intake and utilization of forage, thus making it imperative to evaluate the methods used to determine the nutritive values of forage in order to predict animal performance. Additionally, fibrous feeds for ruminants are less subject to competitive demand. The plant cell wall is the largest hindrance to complete digestion of feeds, particularly forages and by-products and to the utilization of the nutrients and energy they contain, necessitating effective strategies for increasing the rate and efficiency of utilization of forage fiber and the energy therein. It is critically important to increase fiber digestion for ...


Growth Performance, Metabolic And Rumen Profile, Nutrient Utilization, And Health Of Calves Fed Condensed Whey Solubles With Started Pellets, Michaela Joy Della 2019 South Dakota State University

Growth Performance, Metabolic And Rumen Profile, Nutrient Utilization, And Health Of Calves Fed Condensed Whey Solubles With Started Pellets, Michaela Joy Della

Electronic Theses and Dissertations

A study was conducted for this thesis to evaluate the effectiveness of a new product’s ability to improve calf health and growth performance. In addition to the results from the study, this thesis also includes a literature review of immune and rumen maturation in calves and a discussion of ways to protect and encourage those fundamental progressions. For the main research project 48 dairy calves were used in a randomized complete block design study to evaluate the growth performance, rumen fermentation and metabolic profiles, nutrient utilization, and health scores when calves were supplemented Condensed Whey Solubles (CWS) with their ...


Using An Implantable Microchip For Measuring Body Temperature In Dairy Calves, Megan Woodrum, Melissa Cantor, Joao H. C. Costa 2019 University of Kentucky

Using An Implantable Microchip For Measuring Body Temperature In Dairy Calves, Megan Woodrum, Melissa Cantor, Joao H. C. Costa

Posters-at-the-Capitol Presentations

Body temperature is frequently used as a method for determining if illness is present with fever detection. Taking rectal temperature or alternatively tympanic temperature can be time consuming and requires restraint of calves. Alternatives, such as implantable microchips that can be passively read using a radio frequency identification (RFID) scanner may allow for easier monitoring of body temperature.

This study aim was to validate an implantable microchip and to determine the best implant site.


Evaluation Of Flax Oil Supplementation On Performance Of Growing Dairy Heifer Calves And Lactating Cows, Chelsea Rose Schossow 2019 South Dakota State University

Evaluation Of Flax Oil Supplementation On Performance Of Growing Dairy Heifer Calves And Lactating Cows, Chelsea Rose Schossow

Electronic Theses and Dissertations

Utilization of flax oil is new to the livestock industry, and limited research has been done to see the benefits of feeding the highly unsaturated oil to both dairy calves and lactating cows. Two studies were conducted to evaluate the potential benefits of flax oil as a lipid supplement. The first study compared the supplementation of two plant based oils to growing dairy calves. Thirty-six female Holstein calves in individual hutches were used in a 12-wk randomized complete block design study. Treatments were: 1) control (CON) with no oil, 2) 80 g/d of flax oil (FLAX), and 3) and ...


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