Cell and Developmental Biology Commons^™

Quantification Of Mouse Tracheal Epithelial Cell Differentiation With Cilia Defects, Olivia V. Allen

Honors Thesis

Primary Ciliary Dyskinesia (PCD) is an autosomal recessive disorder that affects 1 in every 7,500 live births. PCD results from motile cilia defects, impairing mucociliary clearance and causing chronic respiratory infections. Our lab studies include three PCD mouse lines — bgh (Spef2), nm1054 (Cfap221), and Cfap54^gt/gt (Cfap54). Prior transcriptomic data led us to hypothesize that PCD mutant mouse tracheal epithelial cells (MTECs) exhibit reduced differentiation into ciliated cells. MTECs from the trachea of the Wild Type (WT) and the PCD mutant mouse lines were cultured at an Air Liquid Interface (ALI) to start …

Optimizing Itpa R178c Assays Via Hplc, Jared J. Reisnouer, Wally D. Pines

2026 Symposium

Two of the major nucleotide bases in DNA and RNA (Adenine and Guanine) are derived from the purine Inosine Monophosphate (IMP). IMP may occasionally form the noncanonical nucleotide Inosine Triphosphate (ITP) within the cell and become incorporated into DNA during replication, leading to potentially lethal errors. To combat this, human cells produce the “housekeeping” enzyme Inosine Triphosphatase (ITPA) to revert ITP to IMP. A mutation of this protein that replaces the 178th amino acid Arginine with Cysteine (R178C) is associated with a fatal infantile encephalopathy. Previous assessments of enzyme-substrate binding and catalysis for ITPA variants have been run at a …

Transgenic Plant Cells For The Cost-Effective Production Of Pharmaceutical Proteins, Maria Gambaro

Create@State

Plant-based systems are becoming an important method for producing pharmaceutical proteins because they can be more cost-effective, and safer compared to traditional production methods which commonly use mammalian cells. This project aims to develop a transgenic plant cell system that can produce a pharmaceutical protein using molecular cloning techniques. A transgenic plant cell has the DNA of another plant integrated into its genome, altering its gene expression to produce different proteins. To begin, a plasmid carrying the gene of interest and the DNA components needed for expression will be designed. The plasmid will then be used to introduce the gene …

Exploration Of A Relationship Between The Activities Of Trail And 4’-Trifluoromethoxychalcone., Abigail C. Ernst

Undergraduate Theses

Cancer is the overgrowth of dysregulated or mutated cells that affects 1 in 3 Americans. Chalcones are natural products with many possible derivatives, such as 4’-trifluromethoxychalcone (4TF), developed in Dr. Krzysiak’s lab, which exhibit anticancer activity against cancer cell lines. Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) is a tumor surveillance cytokine that acts on two receptor types: death receptors and decoy receptors. One way cancer cells can become resistant to TRAIL is by upregulating decoy receptors and/or downregulating death receptors. In this study, MTS assays evaluated the potential relationship between TRAIL and 4TF that sensitizes A549 cells to TRAIL. The …

Maintaining And Axenizing Live Cyanobacterial Cultures For Bacterial Genome Sequencing, Yufei Wang, Louisa Acquah

SACAD: Scholarly Activities

Harmful cyanobacterial blooms are an

increasing concern in western Kansas, yet the

bloom-forming species in this region remain

poorly characterized. In this study, we

established and maintained axenic

cyanobacterial cultures to support future

genomic and ecological investigations. By

ensuring low contamination levels and stable

growth conditions, this work provides a

foundation for downstream analyses of bloom-

forming cyanobacteria. It contributes to a better

understanding of freshwater ecosystem health

in western Kansas.

Identification Of Covalent Catechin Adducts Of Enoyl-Acp Reductase (Fabi) From E. Coli Using Mass Spectrometry, Francisco G. Atwi, P. M. Joyner, Rustin Ghidari

Seaver College Research And Scholarly Achievement Symposium

Enoyl-ACP reductase (FabI), an enzyme which is inhibited by catechin, is essential for lipid biosynthesis in bacteria (E. coli). Another FabI inhibitor, triclosan, has strong antibacterial effects,1 but overuse can be detrimental to the environment. Flavonoids, a class of dietary compounds, are known to have potent inhibitory effects on FabI and other kinases.2,3,4 Adducts form as a result of the biochemical reaction between flavonoids and cysteine, a modification which can be verified by m/z shifts on the mass spectra.5 Samples of E. coli protein with overexpressed FabI are utilized: the wild type with ordinary primary structure, and mutant type lacking …

Associations Between The Human Breast Microbiome And Immune Genes In Breast Cancer, Luke Pontoriero, Joan Argwings-Kodhek

Seaver College Research And Scholarly Achievement Symposium

Breast cancer (BC) affects 1 in 8 women and roughly 50% of breast cancers cannot be explained by environmental or genetic factors. There is growing evidence of the role of the human breast microbiome in breast cancer, but the mechanisms guiding this role are not well understood. The co-evolution of the human microbiome with the immune system represents an interaction between host and microbiome that is understudied with regard to breast cancer pathogenesis. We hypothesized that immune genetic variation is associated with altered abundance patterns of bacterial taxa in BC. We conducted 30X whole genome sequencing on a sample of …

Structural Transformation Of A Hydrogel-Forming Cell Division Protein Zapb Of Multidrug Resistant Klebsiella Pneumoniae With Small Molecules, Ayesha Khan, M. Iqbal Choudhary, Sumaira Javaid, Humaira Zafar, Innokentiy Maslennikov, Atia-Tul Wahab

Pharmacy Faculty Articles and Research

Septal ring assembly protein ZapB (EMR18431.1) is involved in Z-ring formation, and nucleoid segregation during the bacterial cell division . It promotes cell division in the initial stages of the cell cycle through direct interaction with FtsZ, thus stimulating Z-ring assembly. The ZapB inhibition can make bacterial cell susceptible to primary antibiotics, and therefore considered as an important approach for adjuvent therapy against MDR bacterial infections. Hence in the current study, ZapB protein of multidrug resistant Klebsiella pneumoniae was cloned, and expressed in E. coli system. To understand its role, and to assess the mechanism of ZapB activation, the characteristic …

Impact Of One Nucleotide On Organ Enumeration And Phyllotaxy In Arabidopsis Thaliana, Hazel R. Frans, Tara Phelps-Durr

SACAD: Scholarly Activities

Arabidopsis thaliana is a model organism used by scientists to study plant genetics, development, and physiology. CRISPR-Cas9 is a biotechnology tool adapted from a bacterial defense mechanism to precisely edit DNA using a guide mRNA and a Cas9 protein. This project aims to create CRISPR-CAS9 mutations in the APETALA3 (AP3) gene of the model plant Arabidopsis thaliana. AP3 is a class B gene critical to the petal and stamen development of Arabidopsis flowers. The AP3 protein contains a MADS domain, which binds directly to DNA and may be responsible for the expression of the CaRG-box genes. AP3 works in conjunction …

Survivin And Caspases Coordinate Proteolytic Cleavage Of The Saga Chromatin Modifying Complex, Elinor Harrison, Alejandro Damian, Cj Talton

Medical Student Research Symposium

Title: Survivin and Caspases Coordinate Proteolytic Cleavage of the SAGA Chromatin Modifying Complex

Elinor Harrison¹, Alejandro Damian¹, CJ Talton¹, Jelly H Soffers¹, Abudu I Bello¹, Kara M Costanzo¹, Joe Bean¹, Ryan D Mohan¹

¹Wayne State University School of Medicine

Background: The Spt Ada Gcn5 Acetyltransferase (SAGA) chromatin modifying complex is a critical regulator of gene expression. Mutation or stoichiometric imbalance of SAGA subunits leads to a spectrum of diseases in model organisms and in humans, including neurodegeneration. SAGA contributes to gene activation through coordinated …

Verifying Mutations In Arabidopsis Thaliana Gene Apetala3, Michelle Wang, Tara Phelps-Durr, Hazel Frans

SACAD: Scholarly Activities

The purpose of this research is to verify the mutation in the Arabidopsis thaliana APETALA3 (AP3) gene. By sequencing DNA, we confirmed the presence of an extra guanine in the mutant AP3 gene. These findings support that further investigation is needed into how the AP3 gene affects flower development in Arabidopsis thaliana.

Cytokinesis And Dbf2, Katharine Gray

Miners Solving for Tomorrow Research Conference

The Mitotic Exit Network (MEN) is a signaling pathway that allows a dividing cell to exit cytokinesis. Dbf2 is a MEN protein kinase in yeast, which provides a simplified model for studying this pathway. By altering Dbf2’s activity through phosphorylation mutants, its effect on cytokinesis can be studied. Transformed cells with a mutant and degron Dbf2 allele are prompted to destroy the degron Dbf2 protein, leaving only mutated Dbf2 behind. This allows for clear analysis of the effect of the phosphorylation mutant on division. Fluorescence microscopy can be used to visualise this degradation, and the impact on actin ring formation …

The Effects Of Preventing Dephosphorylation Of Lqg1 On Cytokinesis, Jenna Mueller

Miners Solving for Tomorrow Research Conference

Iqg1 plays crucial roles in cytokinesis, including the assembly, contraction, and disassembly of the actomyosin ring, as well as the coordination of contraction and septation. Given that cytokinesis must occur post-mitosis, Iqg1 is regulated by the cell cycle. This study explores hypotheses regarding Iqg1 regulation: it’s binding to action is negatively influenced by the cyclin-dependent kinase (CDK) Cdc28 and positively influenced by dephosphorylation via Cdc14. Newly developed yeast degron tagged strains allow for rapid depletion of specific proteins. Iqg1 mutations that alter serine to threonine, which allows phosphorylation but not dephosphorylation, in the degron strain allow testing of mutant phenotypes. …

Targeting Rna Polymerase I In Ewing Sarcoma Treatment, Alexis Bruce

Theses

Ewing sarcoma is a rare and aggressive pediatric bone cancer with poor survival rates in patients, as low as 30% in advanced or recurrent cases. There are limited treatment options, making molecularly targeted therapy vital to explore. Through CRISPR scans of Ewing sarcoma cell lines, ribosome biogenesis was highlighted as a potential target. As RNA polymerase I is a key component of ribosome biogenesis, the cytotoxic ability of two RNA Polymerase I inhibitors, CX-5461 and BMH-21, were used on patient-derived Ewing sarcoma cells. The efficacy of the drugs on two cell lines was determined via measuring cell viability, migration potential, …

Cellular And Molecular Characterization Of Ube3a Function In Cic-Dux4 Sarcoma, Jesse D. Walker

Theses

CIC-DUX4 sarcoma (CDS) is a rare and extremely aggressive soft tissue sarcoma that primarily affects children and young adults. CDS is characterized by an oncogenic chromosomal translocation. Current treatments for CDS are similar to those in other sarcomas like surgery, chemotherapy, and radiation. Treatments, however show that CDS responds poorly compared to other sarcomas, due to relapses of localized disease at high rates and short duration response of treatment, proving the importance of understanding CIC-DUX4’s complex role on how it regulates overall gene expression in CDS cells from a molecular approach.

UBE3A is a ubiquitin protein ligase that plays a …

Effects Of Tubulin-Binding Medications On Microplastic Uptake And Cyotoskeletal Functionality Across Diverse Cell Types, Alejandra D. Favela Santos

Posters - 2026

• Microtubules are composed of of a- and b- tubulin heterodimers, vital for cell structure, shape, and intracellular transport • Microtubules are also useful as they separate chromosomes during cell division • Microplastics (< 5 mm) are considered a health threat and have been found throughout the whole body (1) o In several studies, microplastics have been correlated to higher rates cardiorespiratory issues, endocrine disruption, and cancer (1) • Tubulin-binding medications are widely used as chemotherapeutic agents as they are known for disrupting cell division and targeting tubulin (2,3) o In this study, these medications were used to determine whether microtubules play a role in microplastic uptake § Colchicine typically binds to tubulins and arrests polymerization and growth § Nocodazole disrupts polymerization and prevents spindle formation § Vinblastine inhibits microtubule assembly and disruptions miotic spindle formation.

• The purpose of this study was to determine if different concentrations of tubulin-binding medications affect microplastic uptake o C2C12, RAW 264.7, and MOVAS cell lines were used to evaluate whether these changes are cell type specific 

If microtubule dynamics are disrupted, microplastic uptake can be affect in a concentration-dependent and cell type- specific manner.

Signaling Response Of Epinephrine Treated Cards Toxin-Infected Thp-1 Macrophages, Trinity Martinez

Posters - 2026

Aspiration pneumonia is a common mild case of pneumonia, a disease where the lungs are inflamed and filled with fluid, compromising respiration and gas exchange. One common cause of mild pneumonia is bacterial infection via a pathogen known as Mycoplasma pneumoniae. M. pneumoniae causes a variety of respiratory illness- ranging from pharyngitis, tracheobronchitis, mild pneumonia- and in rare cases encephalitis. M. pneumoniae produces the CARDS toxin, which participates in ADP-ribosylation and vacuolization (Kannan et al., 2006)1. The CARDS toxin causes aspiration pneumonia by paralyzing the cilia that lines the respiratory tract. Epinephrine, or Adrenaline- is a hormone that is medically …

Effects Of Mycoplasma Pneumoniae Cards Toxin On Lung Fibroblast Function, Shuri Magdalene

Posters - 2026

Mycoplasma pneumoniae is a common respiratory pathogen whose virulence depends largely on the community-acquired respiratory distress syndrome (CARDS) toxin, a 591-amino-acid ADP-ribosylating protein¹. CARDS toxin induces immune responses²˒³, causes vacuolation in lung epithelial cells, and contributes to the inflammatory and pathological features of pneumonia. IMR-90 fibroblasts. IMR-90 cells are human fetal lung fibroblasts widely used to study responses to inflammatory signals, extracellular matrix production, and lung injury or repair. Rationale. Fibroblasts are key drivers of tissue remodeling and fibrosis during lung injury. Defining how CARDS toxin influences fibroblast programs in inflammation, cell-cycle control, and matrix remodeling²˒³ will clarify how M. …

Evaluating The Effects Of Akt Knockdown And Everolimus Treatment On Ewing Sarcoma, Arisha Arif, Alfie Barcenez, Nicole Vanegas-Riddick

Posters - 2026

The purpose of this study is to use the gene AKT1, due to the interest in AKT1’s role in cancer cell proliferation, and the drug Everolimus, to determine if combined targeted therapy works as a more efficient therapeutic approach. We hypothesize that the knockdown of AKT1 will increase the sensitivity of Ewing sarcoma cells to Everolimus, resulting in reduced proliferation and/or survival compared to drug treatment alone. This would suggest that AKT1 normally protects cells from drug-induced stress. Ewing Sarcoma has been connected to chromosomal translocations and most common in pediatric patients. It is most often treated with chemotherapy and …

Impact Of Ashwagandha Treatment On Cytokine Production And Macrophage Differentiation Into Thp-1 Cells, Betsi Diaz Chavarin, Aditi Kumar

Posters - 2026

Inflammation is a critical biological response that enables the immune system to react to infections, injuries, or stressful situations. However, chronic or excessive inflammation can lead to tissue damage and contribute to a wide range of diseases, including neurodegenerative disorders, metabolic syndromes, and immune dysregulation. 3,4 Interleukin-1 beta (IL-1β) is a key pro-inflammatory cytokine that plays a central role in initiating and amplifying the immune response. 1,2 It is primarily produced by activated monocytes and macrophages in response to stimuli such as pathogens or stress signals. In addition, IL-1β acts on the hypothalamus to induce fever, which creates an environment …