Plant Breeding and Genetics Commons^™

Preserving Rice Quality: Fine-Mapping And Introgressing A Fissure Resistance Locus, Haley Marie Sater

Graduate Theses and Dissertations

Rice (Oryza sativa L.) kernel fissuring is a major concern of both rice producers and millers. Fissures are small cracks in rice kernels that increase breakage of kernels when milled, and decrease the value of processed rice. This study employed molecular gene tagging methods to fine-map a fissure resistance (FR) locus found in ‘Cybonnet’, a semidwarf tropical japonica cultivar, as well as transfer this trait to rice genotypes of taller, non-semidwarf plant height that are better adapted to some rice production systems. Three QTLs for FR were previously reported; the FR locus with strongest effect resides near the semidwarf sd-1 …

Influence Of Fatty Acids And Their Derivatives On Aphid Resistance In Arabidopsis And Tomato, Jiamei Li

Graduate Theses and Dissertations

Fatty acid desaturases (FADs) are enzymes that act in the chloroplast or the endoplasmic reticulum (ER) to incorporate double bonds into the acyl chains of fatty acids, and recent evidence indicates that at least one of these enzymes, FAD7, also influences plant resistance to aphids. FAD7 is an enzyme in the chloroplast that is found throughout the plant kingdom and that desaturates 16- and 18-carbon fatty acids (FAs) with two double bonds (dienoic acids) to generate FAs with three double bonds (trienoic acids). In tomato (Solanum lycopersicum) and the model plant Arabidopsis thaliana, mutants with impaired FAD7 function are more …

Genetic Analysis Of Soybean Mosaic Virus (Smv) Resistance Genes In Soybean [Glycine Max (L.) Merr.], Mariola Klepadlo

Graduate Theses and Dissertations

Soybean mosaic virus (SMV) causes the most serious viral disease in soybean worldwide. Seven SMV strains, G1 - G7, and three independent multi-allelic loci for SMV resistance, Rsv1, Rsv3, and Rsv4, have been identified. In the initial study, 299 soybean germplasm lines were genotyped for Rsv4 region, inoculated with SMV-G1 and G7 strains, and classified into several resistance groups. The Glyma.02g121400 locus was sequenced from ten soybean accessions, and alignment of the sequences revealed three SNPs displaying 100% polymorphic consistency when a soybean genotype carrying the Rsv4 gene was present. A cross between V94-5152 × Lee 68 was made to …

Is A Mitochondrial Plasmid Really A Virus?, Mackenzie Strehle

UCARE: Research Products

In addition to containing a large and complex mitochondrial genome, the mitochondria of several species of plants have been shown to contain an independent, self-replicating DNA molecule in the form of a plasmid. Plants in the Brassica genus contain a linear plasmid that is approximately 11.6 kilobases in length. The plasmid is characterized by the presence of terminal inverted repeats and covalently bonded proteins at its termini (Handa 2008). The plasmid also contains six ORFs that encode DNA and RNA polymerases and a number of unknown proteins (Figure 1). Currently, both the function of this plasmid and the mechanisms by …

Transgenerational Plasticity Is Sex-Dependent And Persistent In Yellow Monkeyflower (Mimulus Guttatus), Kayla C. Akkerman, Arash Sattarin, John K. Kelly, Alison G. Scoville

Student Published Works

Transgenerational phenotypic plasticity, whereby environmental cues experienced by parents alter the phenotype of their progeny, has now been documented in diverse organisms. Transmission of environmentally determined responses is known to occur through both maternal and paternal gametes, but the underlying mechanisms have rarely been compared. In addition, the persistence of induction over multiple generations appears to vary widely, but has been characterized for relatively few systems. Yellow monkeyflower (Mimulus guttatus) is known to exhibit transgenerational induction of increased glandular trichome production in response to simulated insect damage. Here, we test for differences between maternal and paternal transmission of …

Bio-Piracy: Creating Proprietary Rights In Plant Genetic Resources, James O. Odek

Journal of Intellectual Property Law

No abstract provided.

Identifying Rare Fhb-Resistant Segregants In Intransigent Backcross And F2 Winter Wheat Populations, Anthony J. Clark, Daniela Sarti-Dvorjak, Gina Brown-Guedira, Yanhong Dong, Byung-Kee Baik, David A. Van Sanford

Plant and Soil Sciences Faculty Publications

Fusarium head blight (FHB), caused mainly by Fusarium graminearum Schwabe [telomorph: Gibberella zeae Schwein.(Petch)] in the US, is one of the most destructive diseases of wheat (Triticum aestivum L. and T. durum L.). Infected grain is usually contaminated with deoxynivalenol (DON), a serious mycotoxin. The challenge in FHB resistance breeding is combining resistance with superior agronomic and quality characteristics. Exotic QTL are widely used to improve FHB resistance. Success depends on the genetic background into which the QTL are introgressed, whether through backcrossing or forward crossing; QTL expression is impossible to predict. In this study four high-yielding soft red …

Next Generation Mapping Of Enological Traits In An F2 Interspecific Grapevine Hybrid Family, Shanshan Yang, Jonathan Fresnedo-Ramírez, Qi Sun, David C. Manns, Gavin L. Sacks, Anna Katherine Mansfield, James L. Luby, Jason Londo, Bruce I. Reisch, Lance Cadle-Davidson, Anne Y. Fennell

Agronomy, Horticulture and Plant Science Faculty Publications

In winegrapes (Vitis spp.), fruit quality traits such as berry color, total soluble solids content (SS), malic acid content (MA), and yeast assimilable nitrogen (YAN) affect fermentation or wine quality, and are important traits in selecting new hybrid winegrape cultivars. Given the high genetic diversity and heterozygosity of Vitis species and their tendency to exhibit inbreeding depression, linkage map construction and quantitative trait locus (QTL) mapping has relied on F₁ families with the use of simple sequence repeat (SSR) and other markers. This study presents the construction of a genetic map by single nucleotide polymorphisms identified through genotyping-by-sequencing …

Characterizing Genotype X Management Interactions On Soybean Seed Yield, David A. Marburger, Bryson J. Haverkamp, Randall G. Laurenz, John M. Orlowski, Eric W. Wilson, Shaun N. Casteel, Chad D. Lee, Seth L. Naeve, Emerson D. Nafziger, Kraig L. Roozeboom, William J. Ross, Kurt D. Thelen, Shawn P. Conley

Plant and Soil Sciences Faculty Publications

Increased soybean [Glycine max (L.) Merr.] commodity prices in recent years have generated interest in high-input systems to increase yield. The objective of this study was to evaluate the effects of current, high-yielding cultivars under high- and low-input systems on soybean yield and yield components. Research trials were conducted at 19 locations spanning nine states from 2012 to 2014. At each location, six high-yielding cultivars were grown under three input systems: (i) standard practice (SP, current recommended practices), (ii) high-input treatment consisting of a seed treatment fungicide, insecticide, nematistat, inoculant, and lipo-chitooligosaccharide (LCO); soil-applied N fertilizer; foliar LCO, fertilizer, …

Dedicated Industrial Oilseed Crops As Metabolic Engineering Platforms For Sustainable Industrial Feedstock Production, Li-Hua Zhu, Frans Krens, Mark A. Smith, Xueyuan Li, Weicong Qi, Elbertus N. Van Loo, Tim Iven, Ivo Feussner, Tara J. Nazarenus, Dongxin Huai, David C. Taylor, Xue-Rong Zhou, Allan G. Green, Jay Shockey, K. Thomas Klasson, Robert T. Mullen, Bangquan Huang, John M. Dyer, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant …

Ginkgo And Welwitschia Mitogenomes Reveal Extreme Contrasts In Gymnosperm Mitochondrial Evolution, Wenhu Guo, Felix Grewe, Weishu Fan, Gregory J. Young, Volker Knoop, Jeffrey D. Palmer, Jeffrey P. Mower

Center for Plant Science Innovation: Faculty Publications

Mitochondrial genomes (mitogenomes) of flowering plants are well known for their extreme diversity in size, structure, gene content, and rates of sequence evolution and recombination. In contrast, little is known about mitogenomic diversity and evolution within gymnosperms. Only a single complete genome sequence is available, from the cycad Cycas taitungensis, while limited information is available for the one draft sequence, from Norway spruce (Picea abies). To examine mitogenomic evolution in gymnosperms, we generated complete genome sequences for the ginkgo tree (Ginkgo biloba) and a gnetophyte (Welwitschia mirabilis). There is great disparity in size, …

Effect Of Cry1f Maize On The Behavior Of Susceptible And Resistant Spodoptera Frugiperda And Ostrinia Nubilalis, Ana María Vélez, Analiza P. Alves, Erin E. Blankenship, Blair Siegfried

Department of Entomology: Faculty Publications

Understanding the behavior of pests targeted with Bacillus thuringiensis Berliner (Bt) crops is important to define resistance management strategies. Particularly the study of larval movement between plants is important to determine the feasibility of refuge configurations. Exposure to Bt maize, Zea mays L. (Poaceae), has been suggested to increase larval movement in lepidopteran species but few studies have examined the potential for resistance to interact with behavioral responses to Bt toxins. Choice and no-choice experiments were conducted with Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) and Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) to determine whether Cry1F resistance influences neonate movement. Leaf discs …

Effect Of Green Tea On Interaction Of Lipid Oxidation Products With Sarcoplasmic And Myofibrillar Protein Homogenates Extracted From Bovine Top Round Muscle, Nahathai Stapornkul, Tatiana Prytkova, Lilian Were

Food Science Faculty Articles and Research

The interaction between lipid oxidation products and bovine sarcoplasmic (SP) and myofibrillar protein (MP) homogenates in the presence of green tea was investigated. To monitor the effect of green tea on lipid oxidation, aldehydes were measured while effect on protein was monitored via changes in myoglobin, thiols, and tryptophan fluorescence over nine days of refrigerated storage. The presence of SP and MP decreased free aldehydes in the buffers. The SP bound more aldehydes than MP. The tea compounds exhibited more favorable binding energies than aldehydes near histidine 64 close to the heme moiety of myoglobin. Addition of tea lowered tryptophan …

A Bacterial Effector Co-Opts Calmodulin To Target The Plant Microtubule Network, Ming Guo, Panya Kim, Guangyong Li, Christian Elowsky, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The bacterial pathogen Pseudomonas syringae depends on effector proteins secreted by its type III secretion system for the pathogenesis of plants. The majority of these effector proteins are known suppressors of immunity, but their plant targets remain elusive. Using Arabidopsis thaliana as a model host, we report that the HopE1 effector uses the host calcium sensor, calmodulin (CaM), as a co-factor to target the microtubule- associated protein 65 (MAP65), an important component of the microtubule network. HopE1 interacted with MAP65 in a CaMdependent manner, resulting in MAP65-GFP dissociation from microtubules. Transgenic Arabidopsis expressing HopE1 had reduced secretion of the immunity …

Screen Of Non-Annotated Small Secreted Proteins Of Pseudomonas Syringae Reveals A Virulence Factor That Inhibits Tomato Immune Proteases, Takayuki Shindo, Farnusch Kaschani, Fan Yang, Judit Kovács, Fang Tian, Jiorgos Kourelis, Tram Ngoc Hong, Tom Colby, Mohammed Shabab, Rohini Chawla, Selva Kumari, Muhammed Ilyas, Anja C. Hörger, James R. Alfano, Renier A. L. Van Der Hoorn

Center for Plant Science Innovation: Faculty Publications

Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. Here we identified 131 candidate small, secreted, non-annotated proteins from the PtoDC3000 genome, most of which are common to Pseudomonas species and potentially expressed during apoplastic colonization. We produced 43 of these proteins through a custom-made gateway-compatible expression system for extracellular bacterial proteins, and screened them for their ability to inhibit the secreted immune protease C14 of tomato using competitive activity-based protein profiling. This screen revealed C14-inhibiting protein-1 (Cip1), which contains …

Residues Responsible For The Selectivity Of Α-Conotoxins For Ac-Achbp Or Nachrs, Bo Lin, Shi-Hua Xiang, Mengsen Li

Center for Plant Science Innovation: Faculty Publications

Nicotinic acetylcholine receptors (nAChRs) are targets for developing new drugs to treat severe pain, nicotine addiction, Alzheimer disease, epilepsy, etc. α-Conotoxins are biologically and chemically diverse. With 12–19 residues and two disulfides, they can be specifically selected for different nAChRs. Acetylcholine-binding proteins from Aplysia californica (Ac-AChBP) are homologous to the ligand-binding domains of nAChRs and pharmacologically similar. X-ray structures of the α-conotoxin in complex with Ac-AChBP in addition to computer modeling have helped to determine the binding site of the important residues of α-conotoxin and its affinity for nAChR subtypes. Here, we present the various α-conotoxin residues that are selective …

Screen Of Non-Annotated Small Secreted Proteins Of Pseudomonas Syringae Reveals A Virulence Factor That Inhibits Tomato Immune Proteases, Takayuki Shindo, Farnusch Kaschani, Fan Yang, Judit Kovács, Fang Tian, Jiorgos Kourelis, Tram Ngoc Hong, Tom Colby, Mohammed Shabab, Rohini Chawla, Selva Kumari, Muhammed Ilyas, Anja C. Hörger, James R. Alfano, Renier A. L. Van Der Hoorn

Center for Plant Science Innovation: Faculty Publications

Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. Here we identified 131 candidate small, secreted, non-annotated proteins from the PtoDC3000 genome, most of which are common to Pseudomonas species and potentially expressed during apoplastic colonization. We produced 43 of these proteins through a custom-made gateway-compatible expression system for extracellular bacterial proteins, and screened them for their ability to inhibit the secreted immune protease C14 of tomato using competitive activity-based protein profiling. This screen revealed C14-inhibiting protein-1 (Cip1), which contains motifs of …

Limited Mitogenomic Degradation In Response To A Parasitic Lifestyle In Orobanchaceae, Weishu Fan, Andan Zhu, Melisa Kozaczek, Neethu Shah, Natalia Pabón-Mora, Favio González, Jeffrey P. Mower

Center for Plant Science Innovation: Faculty Publications

In parasitic plants, the reduction in plastid genome (plastome) size and content is driven predominantly by the loss of photosynthetic genes. The first completed mitochondrial genomes (mitogenomes) from parasitic mistletoes also exhibit significant degradation, but the generality of this observation for other parasitic plants is unclear. We sequenced the complete mitogenome and plastome of the hemiparasite Castilleja paramensis (Orobanchaceae) and compared them with additional holoparasitic, hemiparasitic and nonparasitic species from Orobanchaceae. Comparative mitogenomic analysis revealed minimal gene loss among the seven Orobanchaceae species, indicating the retention of typical mitochondrial function among Orobanchaceae species. Phylogenetic analysis demonstrated that the mobile cox1 …

Identification Of Homogentisate Dioxygenase As A Target For Vitamin E Biofortification In Oilseeds, Minviluz G. Stacey, Rebecca E. Cahoon, Hanh T. Nguyen, Yaya Cui, Shirley Sato, Cuong T. Nguyen, Nongnat Phoka, Kerry M. Clark, Yan Liang, Joe Forrester, Josef Batek, Phat Tien Do, David A. Sleper, Thomas E. Clemente, Edgar B. Cahoon, Gary Stacey

Center for Plant Science Innovation: Faculty Publications

Soybean (Glycine max) is a major plant source of protein and oil and produces important secondary metabolites beneficial for human health. As a tool for gene function discovery and improvement of this important crop, a mutant population was generated using fast neutron irradiation. Visual screening of mutagenized seeds identified a mutant line, designated MO12, which produced brown seeds as opposed to the yellow seeds produced by the unmodified Williams 82 parental cultivar. Using forward genetic methods combined with comparative genome hybridization analysis, we were able to establish that deletion of the GmHGO1 gene is the genetic basis of …

Evolutionary Explanation For Invasive Abilities Of Centaurea Stoebe (Spotted Knapweed) In Introduced Areas, Erin Koren

UReCA: The NCHC Journal of Undergraduate Research and Creative Activity

Since every invasion and every exotic species presents a new challenge with novel invasion strategies, the invasion of Centaurea stoebe (synonymously referred to as C. maculosa) into North America will be used as a case study to deeply examine invasion strategies. Centaurea stoebe is a short-lived (approximately 3 years) perennial member of the aster family (Broz et al., 2007). It occurs in two different cytotypes, or with two different chromosomal factors. In its home range, it exists in both diploid and tetraploid cytotypes, but only the tetraploid version is present in introduced ranges in North America (See Figure 1; Treier …

A Molecular Genetic And Physiological Comparison Of The Hybrid Necrosis Response In Wheat (Triticum Aestivum L.) To Biotic And Abiotic Stress Responses, Devi R. Kandel

Electronic Theses and Dissertations

Expression of hybrid necrosis in plants can lead to a significant reduction in productivity, or even lethality. Epistatic interactions between divergent loci that have evolved through evolution are proposed as being responsible for the genetic incompatability that is expressed as hybrid necrosis. Hybrid necrosis can also represent an obstacle to the transfer of desirable traits from wild, related species to domesticated bread wheat. In wheat, expression of dominant complementary genes Ne1 and Ne2, located to chromosomes 5B and 2B in a hybrid results the production of necrotic leaf tissue, stunted plant growth, and reduced grain yield, which are similar plant …

Evaluation And Identification Of Soybean Aphid Resistance Sources And Mapping Of Soybean Aphid Resistance Loci In Early Maturing Soybean Germplasm Accessions, Siddhi Jeewan Bhusal

Electronic Theses and Dissertations

Soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America particularly in the northern United States and three Canadian provinces. At least four biotypes of soybean aphid have been confirmed in the United States. Identification of soybean aphid resistance sources in early-maturing soybeans and genetic characterization of new sources of resistance will facilitate to expand the gene pool of soybean aphid resistance and thus will help to develop soybean aphid resistant cultivars. To identify new sources of resistance in early maturing soybeans, 330 soybean germplasm accessions from Maturity Group (MG) I, …

Snrna 3' End Processing By A Cpsf73- Containing Complex Essential For Development In Arabidopsis, Yunfeng Liu, Shengjun Li, Yuan Chen, Athen N. Kimberlin, Edgar B. Cahoon, Bin Yu

Center for Plant Science Innovation: Faculty Publications

Uridine-rich small nuclear RNAs (snRNAs) are the basal components of the spliceosome and play essential roles in splicing. The biogenesis of the majority of snRNAs involves 3’ end endonucleolytic cleavage of the nascent transcript from the elongating DNA-dependent RNA ploymerase II. However, the protein factors responsible for this process remain elusive in plants. Here, we show that DEFECTIVE in snRNA PROCESSING 1 (DSP1) is an essential protein for snRNA 3’ end maturation in Arabidopsis. A hypomorphic dsp1-1 mutation causes pleiotropic developmental defects, impairs the 3’ end processing of snRNAs, increases the levels of snRNA primary transcripts (pre-snRNAs), and alters …

Evolutionary Dynamics Of The Plastid Inverted Repeat: The Effects Of Expansion, Contraction, And Loss On Substitution Rates, Andan Zhu, Wenhu Guo, Weishu Fan, Jeffrey P. Mower

Center for Plant Science Innovation: Faculty Publications

• Rates of nucleotide substitution were previously shown to be several times slower in the plastid inverted repeat (IR) compared with single-copy (SC) regions, suggesting that the IR provides enhanced copy-correction activity.

• To examine the generality of this synonymous rate dependence on the IR, we compared plastomes from 69 pairs of closely related species representing 52 families of angiosperms, gymnosperms, and ferns.

• We explored the breadth of IR boundary shifts in land plants and demonstrate that synonymous substitution rates are, on average, 3.7 times slower in IR genes than in SC genes. In addition, genes moved from the SC into the …

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Efficiency Of The Nuclease I-Scei In Excising Selectable Marker Genes From The Plant Genome, Elliot E. Pruett, Soumen Nandy, Vibha Srivastava

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Gene stacking is a method used in biotechnology by which multiple genes can be placed at a single genomic site, thereby simplifying plant breeding. In this approach, DNA nucleases are used for excising selectable marker genes (SMG), which are the unneeded components of transgenic plants. The goal of this project is to evaluate the effectiveness of the nuclease I-SceI in excising DNA in plants. Specifically, this study tests heat-inducible I-SceI through the use of a heat-shock promoter (HS) in order to control SMG excision by heat application. The DNA plasmid containing a visual marker gene flanked by I-SceI target sites …

Evaluation Of Harvest Time/Temperature And Storage Temperature On Postharvest Incidence Of Red Drupelet Reversion Development And Firmness Of Blackberry (Rubus L. Subgenus Rubus Watson), Jack E. Mccoy, John R. Clarke, Alejandra A. Salgado, Andrew Jecmen

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Since 1964, the University of Arkansas blackberry breeding program has worked to improve fruit quality and shipping capabilities. A major limitation in blackberry fruit is postharvest handling potential for the shipping market. Maintaining fruit firmness in storage is crucial. Red drupelet reversion (or simply reversion) is also an important postharvest disorder in which drupelets change from black to red during storage. It is hypothesized that reversion is increased when fruit is picked at hot temperatures and exposed to a rapid change of temperature. These studies evaluated harvest time/temperature, as well as storage temperature, on berry firmness and the incidence of …

Toward The Elucidation Of Cytoplasmic Diversity In North American Grape Breeding Programs, Jonathan Fresnedo-Ramirez, Qi Sun, Chin-Feng Hwang, Craig A. Ledbetter, David W. Ramming, Anne Fennell, M. Andrew Walker, James J. Luby, Matthew D. Clark, Jason P. Londo, Lance Cadle-Davidson, Gan-Yuan Zhong

Agronomy, Horticulture and Plant Science Faculty Publications

Plants have an intriguing tripartite genetic system: Nuclear genome 9 Mitochondria 9 Plastids and their interactions may impact germplasm breeding. In grapevine, the study of cytoplasmic genomes has been limited, and their role with respect to grapevine germplasm diversity has yet to be elucidated. In the present study, the results of an analysis of the cytoplasmic diversity among 6073 individuals (comprising cultivars, interspecific hybrids and segregating progenies) are presented. Genotyping by sequencing (GBS) was used to elucidate plastid and mitochondrial DNA sequences, and results were analyzed using multivariate techniques. Single nucleotide polymorphism (SNP) effects were annotated in reference to plastid …

Field Evaluation Of Burley Lines Containing Alleles Minimizing Nicotine To Nornicotine Conversion, Cameron G. Shelton

Theses and Dissertations--Plant and Soil Sciences

CYP82e4, CYP82e5, and CYP82e10 mutant alleles that minimize the conversion of nicotine to nornicotine have been introgressed into numerous existing low converting (LC) burley varieties and parental lines developed by the Kentucky-Tennessee tobacco breeding program. A backcross breeding protocol was utilized, with the objective being the creation of "e3" varieties that differed from their LC counterparts only for nornicotine and nitroso-nornicotine content. Field studies were conducted in Kentucky and Tennessee during the 2013 growing season, with 17 prospective parental lines and 20 prospective commercial varieties grown and compared to their original counterparts. Most of the e3 lines were not morphologically …

Midwest Vegetable Trial Report For 2015, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

This is a compilation of 19 research trial reports from six land-grant universities in the midwestern United States. Crops include bok choy, cantaloupe, cucumber, pepper, pumpkin, sweet corn, squash, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties, including one investigating cantaloupe variety influence on cucumber beetle presence and incidence of bacterial wilt. Four reports addressed specific growing practices: the use of poly-coated urea to supply nitrogen to sweet corn, biochar use in a high tunnel bag culture system, use of cereal rye cover crop in butternut squash, and pollinizer …

Mini- And Heirloom Sweet Pepper Variety Performance In High Tunnels, 2015, Elizabeth Maynard, Israel S. Calsoyas

Purdue Fruit and Vegetable Research Reports

Trials on mini sweet peppers and other specialty sweet peppers in the Midwest have not recently been reported. We undertook this project to compare yield and fruit characteristics of hybrid and heirloom peppers grown in high tunnels using organic and conventional production methods.