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Full-Text Articles in Plant Breeding and Genetics

Oestrogenic Subterranean Clover Guide. Identification And Remediation, Kevin J. Foster, Megan H. Ryan, Daniel R. Kidd May 2022

Oestrogenic Subterranean Clover Guide. Identification And Remediation, Kevin J. Foster, Megan H. Ryan, Daniel R. Kidd

Bulletins 4000 -

Subterranean clover (subclover, Trifolium subterraneum L.) is an annual pasture legume that provides high quality feed for livestock. It is estimated to be sown over 30 million hectares across southern Australia, with cultivars developed for the low, medium and high rainfall zones. Unfortunately, some older cultivars contain high levels of phytoestrogen which can affect the reproductive system of sheep and cause other health issues. This became a significant issue for sheep producers from the 1940s as new land was cleared and sheep grazed subclover-dominant pastures. There was a collection of livestock issues observed and these were grouped together under the …


Enabling Breeding For Targeted Heat Requirement In Peach, Omer Atagul May 2022

Enabling Breeding For Targeted Heat Requirement In Peach, Omer Atagul

All Theses

Peach [Prunus persica (L.) Batsch] germplasm varies in heat requirement and bloom time. This variability can be utilized in breeding for targeted environment or overall climate resilience. Development of molecular tools to aid in targeted selection of dormancy related traits is needed to support breeding for these traits. This project furthers our understanding of heat requirement accumulation diversity and genetic control in peach.

Peach bloom date (BD) is determined by the dynamic relationship of temperature requirements, from the sequential fulfillment of chill requirement (CR) and heat requirement (HR). Knowledge of HR and its influence on BD would provide deeper …


Genetic Control Of Prickles And Plant Height In Blackberry, Carmen Johns May 2022

Genetic Control Of Prickles And Plant Height In Blackberry, Carmen Johns

Graduate Theses and Dissertations

The fresh-market blackberry (Rubus L. subgenus Rubus Watson) industry has been expanding for the past two decades. Blackberry market growth can be attributed to many factors including expanded production regions, improved production systems, and release of superior cultivars. Blackberry breeding is a time- and labor-intensive process which would benefit from implementation of molecular markers for highly heritable, important traits. Next generation sequencing, a high-quality reference genome, and software capable of analyzing this complex genome were applied in a genome-wide association study (GWAS) to reveal maker-trait associations related to prickles and plant height variation in fresh-market blackberry. In 2020 and 2021, …


Arkansas Fresh-Market Blackberries: Identifying Unique Attributes And Harvest Practices That Impact Marketability, Andrea Lea Myers May 2022

Arkansas Fresh-Market Blackberries: Identifying Unique Attributes And Harvest Practices That Impact Marketability, Andrea Lea Myers

Graduate Theses and Dissertations

Fresh-market blackberries (Rubus subgenus Rubus) are sold worldwide and have attributes that appeal to consumers. The delicate-nature of the fruit requires hand harvesting, and minimal handling of the fruit postharvest. Objectives of this research on Arkansas fresh-market blackberries was to 1) identify the unique attributes 2) determine the best handling practices to increase postharvest quality, and 3) evaluate the potential of soft robotic gripper for harvesting. For the first objective, blackberry genotypes were harvested from the University of Arkansas System (UA System) Division of Agriculture Fruit Research Station in Clarksville, AR. Nineteen genotypes were harvested in 2020, eight genotypes were …


Breeding Soybean [Glycine Max (L) Merr.] Under Reduced Irrigation, Francia Seconde Ravelombola May 2022

Breeding Soybean [Glycine Max (L) Merr.] Under Reduced Irrigation, Francia Seconde Ravelombola

Graduate Theses and Dissertations

Soybean [Glycine max (L) Merr.], a legume species native to East Asia in the Fabaceae family, ranks among the most important food crops in the world. It is widely grown and known for its high protein and oil concentration. Soybean is valuable because its seeds have multiple applications in food, feed, pharmaceutical, and industrial enterprises. Even though seed yield is the most important trait, breeders have recently given a significant attention to quality traits, such as high protein or modified oil concentration. Soybean seed protein inheritance has been extensively studied; however, genetics of high-protein ‘BARC-7’ soybean are still unknown.On the …


Managing Stress Tolerance On Warm-Season Putting Greens In The Transition Zone, Thomas Edward Walton May 2022

Managing Stress Tolerance On Warm-Season Putting Greens In The Transition Zone, Thomas Edward Walton

Graduate Theses and Dissertations

The use of warm-season putting greens in the transition zone has increased in recent years. Ultradwarf bermudagrass (Cynodon dactylon (L.) Pers x C. transvaalensis Burtt-Davy) is the most prevalent warm-season putting green selection in the transition zone, however, newly developed greens-type zoysiagrass (Zoysia spp.) cultivars represent another potential selection for golf courses. The two major limitations of warm-season grasses in the transition zone are a general lack of cold- and shade-tolerance. Protective covers are essential to protect ultradwarf bermudagrass putting greens in the winter months. Unfortunately, golf courses can still experience winterkill underneath protective covers. Two field trials were conducted …


Genetic Diversity In Helianthus Annuus: Selective Breeding And Induced Mutagenesis, Dillon Holton Apr 2022

Genetic Diversity In Helianthus Annuus: Selective Breeding And Induced Mutagenesis, Dillon Holton

Thinking Matters Symposium

H. annuus, more commonly known as the sunflower, is a single species that has many different variations. Traits have been selectively bred for hundreds of years in order to produce a beautiful flower as well as essential agricultural products. Recently interest in the sunflower as a food and fuel crop has led to selective breeding of seeds that produce high yields of sunflower oil. To further push the limits of this plant's genome, researchers have used mutagenesis to force mutations in hopes of developing novel modifications that could increase yield. With the entire genome mapped, the goal for many research …


Pengujian Karakter Hasil Dan Komponen Hasil Klon Ubi Jalar Berdaging Putih Berdasarkan Analisis Multivariat, Arif Affan Wicaksono, Debby Ustari, Saskia Pratiwi, Syariful Mubarok, Agung Karuniawan Apr 2022

Pengujian Karakter Hasil Dan Komponen Hasil Klon Ubi Jalar Berdaging Putih Berdasarkan Analisis Multivariat, Arif Affan Wicaksono, Debby Ustari, Saskia Pratiwi, Syariful Mubarok, Agung Karuniawan

Jurnal Kultivasi

Ubi jalar memiliki keragaman genetik yang luas sehingga potensi genetiknya dapat dikembangkan lebih lanjut. Ubi jalar berdaging putih memiliki kandungan pati paling tinggi dibandingkan dengan warna yang lain. Tujuan dari penelitian ini adalah untuk mengetahui variasi karakter hasil dan komponen hasil klon ubi jalar berdaging putih. Percobaan dilakukan di tiga lokasi sentra produksi ubi jalar yakni Kabupaten Sumedang, Kabupaten Garut, dan Kabupaten Majalengka, Jawa Barat mulai bulan Desember 2019 hingga Desember 2020. Percobaan dilakukan dengan menguji 11 klon ubi jalar berdaging putih, yang terdiri atas 8 klon hasil persilangan dan 3 varietas cek menggunakan Rancangan Acak Kelompok dengan tiga ulangan. …


Marker-Trait Association Mapping Of Sorghum Mosaic Virus Resistance In Sugarcane, Chenie Zamora Apr 2022

Marker-Trait Association Mapping Of Sorghum Mosaic Virus Resistance In Sugarcane, Chenie Zamora

LSU Master's Theses

Mosaic is a potentially devastating disease of sugarcane that had severe economic impact on Louisiana’s sugarcane industry in the 1920’s and has caused periodic problems ever since. The disease is vectored by migrating aphids that transmit Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus (SrMV) in a non-persistent manner. Mosaic in Louisiana is currently caused by strains of SrMV. Mosaic has been controlled primarily by developing resistant cultivars. Recently, mosaic was detected in some near-commercial clones in the cultivar selection program. This prompted extensive screening of breeding program parent populations that detected infiltration of susceptibility and revealed the need for …


Identification And Characterization Of Circular Rnas In Brassica Rapa In Response To Plasmodiophora Brassicae, Huishan Liu, Chinedu Charles Nwafor, Yinglan Piao, Xiaonan Li, Zongxiang Zhan, Zhongyun Piao Apr 2022

Identification And Characterization Of Circular Rnas In Brassica Rapa In Response To Plasmodiophora Brassicae, Huishan Liu, Chinedu Charles Nwafor, Yinglan Piao, Xiaonan Li, Zongxiang Zhan, Zhongyun Piao

Center for Plant Science Innovation: Faculty Publications

Plasmodiophora brassicae is a soil-borne pathogen that attacks the roots of cruciferous plants and causes clubroot disease. CircRNAs are noncoding RNAs, widely existing in plant and animal species. Although knowledge of circRNAs has been updated continuously and rapidly, information about circRNAs in the regulation of clubroot disease resistance is extremely limited in Brassica rapa. Here, Chinese cabbage (BJN 222) containing clubroot resistance genes (CRa) against P. brassicae Pb4 was susceptible to PbE. To investigate the mechanism of cicRNAs responsible for clubroot disease resistance in B. rapa, circRNA-seq was performed with roots of ‘BJN 222’ at 0, 8, and 23 days …


Potensi Hasil Dan Uji Keseragaman Famili F7 Padi Gogo Tahan Rebah Hasil Persilangan Padi Lokal Bangka X Varietas Unggul, Eries Dyah Mustikarini, Gigih Ibnu Prayoga, Ratna Santi, Widodo Wisnu Murti Apr 2022

Potensi Hasil Dan Uji Keseragaman Famili F7 Padi Gogo Tahan Rebah Hasil Persilangan Padi Lokal Bangka X Varietas Unggul, Eries Dyah Mustikarini, Gigih Ibnu Prayoga, Ratna Santi, Widodo Wisnu Murti

Jurnal Kultivasi

Perakitan tanaman padi gogo yang akan dilepaskan sebagai varietas harus memiliki keseragaman dan kestabilan yang tinggi. Penelitian ini bertujuan untuk mengetahui keseragaman famili F7 pada tanaman padi gogo tahan rebah dan mendapatkan galur harapan dengan daya hasil tertinggi pada tanaman padi gogo F7. Penelitian ini dilaksanakan pada bulan Desember 2020 sampai Mei 2021, di Kebun Penelitian dan Percobaan (KP2), Fakultas Pertanian Perikanan dan Biologi, Universitas Bangka Belitung. Penelitian ini menggunakan metode eksperimen dengan rancangan percobaan berupa rancangan acak kelompok yang diulang sebanyak 3 kali. Perlakuan terdiri dari 5 galur F7 padi gogo dan 5 varietas pembanding. Analisis data karakter kualitatif …


Potensi Hasil Dan Nutrisi Beberapa Genotipe Tanaman Sorgum Lokal Jawa Timur Sebagai Calon Tetua Persilangan, Maftuchah, Dian Puji Rahayu, Agus Zainudin, Sulistyawati, Hendry Sulistiyanto Apr 2022

Potensi Hasil Dan Nutrisi Beberapa Genotipe Tanaman Sorgum Lokal Jawa Timur Sebagai Calon Tetua Persilangan, Maftuchah, Dian Puji Rahayu, Agus Zainudin, Sulistyawati, Hendry Sulistiyanto

Jurnal Kultivasi

Tanaman sorgum (Sorghum bicolor (L.) Moench) merupakan tanaman sumber pangan, pakan ternak, dan bahan baku industri yang memiliki potensi untuk dikembangkan di Indonesia. Permasalahan utama dalam komoditas ini adalah masih terbatasnya varietas lokal yang sesuai dengan lingkungan di Indonesia sehingga perlu diupayakan pencarian genotipe lokal sorgum sebagai calon tetua persilangan. Tujuan kegiatan ini adalah untuk mendapatkan informasi potensi daya hasil dan kualitas produk berbagai genotipe sorgum lokal Jawa Timur sebagai calon tetua persilangan untuk menghasilkan varietas unggul. Penelitian ini dilaksanakan di lahan percobaan Desa Purutrejo, Kecamatan Purworejo, Kota Pasuruan-Jawa Timur. Bahan yang digunakan dalam penelitian ini yaitu 9 genotipe tanaman …


Perbandingan Daya Hasil Dan Toleransi Naungan Berbagai Genotipe Jagung Padjadjaran Pada Naungan Eukaliptus, Adilah Nurul Fitrah, Nono Carsono, Dedi Ruswandi Apr 2022

Perbandingan Daya Hasil Dan Toleransi Naungan Berbagai Genotipe Jagung Padjadjaran Pada Naungan Eukaliptus, Adilah Nurul Fitrah, Nono Carsono, Dedi Ruswandi

Jurnal Kultivasi

Kebutuhan jagung sebagai bahan pangan, pakan dan industri selalu meningkat, namun terdapat kendala dalam produksi jagung domestik, yaitu konversi lahan pertanian. Sistem agroforestri eukaliptus-jagung dapat menjadi alternatif yang digunakan untuk pengembangan jagung. Tujuan penelitian ini untuk mengidentifikasi daya hasil, mengestimasi nilai indeks toleransi, dan menyeleksi galur jagung Padjadjaran yang toleran terhadap naungan Eucalyptus sp. Penelitian ini dilaksanakan pada April – November 2019 di Sanggar Penelitian Latihan dan Pengembangan Pertanian (SPLPP) Fakultas Pertanian Universitas Padjadjaran, Bandung. Rancangan percobaan menggunakan rancangan petak terbagi (Split plot) dengan dua faktor (naungan dan genotipe) dan tiga ulangan. Petak utama adalah faktor naungan yang terdiri dari …


Genetic Characterization Of Resistance To Bacterial Panicle Blight And Sheath Blight In Rice Using Qtl Linkage Analysis And Qtl-Seq, John Christian E. Ontoy Apr 2022

Genetic Characterization Of Resistance To Bacterial Panicle Blight And Sheath Blight In Rice Using Qtl Linkage Analysis And Qtl-Seq, John Christian E. Ontoy

LSU Master's Theses

Bacterial panicle blight (BPB) and sheath blight (SB) are major diseases of rice causing significant yield reduction in conducive conditions. Few cultivars have shown reliable resistance to these diseases. In this study, a recombinant inbred line (RIL) population derived from the U.S. cultivars Jupiter (moderately resistant) and Trenasse (susceptible) was investigated to identify genomic regions associated with BPB and SB resistance. Phenotypes in BPB and SB, as well as days to heading (DH), of the RIL population were evaluated in the field. DH was found to be correlated to BPB and SB diseases. BPB was also positively correlated to SB. …


Molecular Evidence Of Cryptic Hybridization In The Japanese Nezasa Bamboos (Pleioblastus Section Nezasa), Ryan Long Apr 2022

Molecular Evidence Of Cryptic Hybridization In The Japanese Nezasa Bamboos (Pleioblastus Section Nezasa), Ryan Long

Theses

The genus Pleioblastus is a complex group of Southeast Asian temperate bamboos with 7-21 species, depending on taxonomic authority. This study tests the hypothesis that taxonomic complexity in this group is due in part to hybridization and subsequent backcrossing in natural populations, resulting in a reticulate evolutionary history and a contemporary species assemblage that includes cryptic hybrids. This hypothesis is supported by recent research on the temperate bamboos that revealed intergeneric hybrids involving Pleioblastus and other genera of temperate bamboos (Triplett and Clark, 2021). The objective of the current study was to use data from Amplified Fragment Length Polymorphism (AFLP) …


Spectral Signature Description Of Some Wheat Genotypes And Study Their Relationship With Yield Prediction Spectraly In The Semi Arid Zones, توصيف البصمة الطيفية لبعض طرز القمح ودراسة علاقتها بتنبؤ الإنتاجية طيفياً في المناطق شبه الجافة, Nasser Ibrahem, Husam Farag Mar 2022

Spectral Signature Description Of Some Wheat Genotypes And Study Their Relationship With Yield Prediction Spectraly In The Semi Arid Zones, توصيف البصمة الطيفية لبعض طرز القمح ودراسة علاقتها بتنبؤ الإنتاجية طيفياً في المناطق شبه الجافة, Nasser Ibrahem, Husam Farag

Arab Journal of Arid Environments المجلة العربية للبيئات الجافة

In this research, the spectral signature was determined within the wavelength range 350-2500 nm at every 1 nm during all growth stages of some wheat genotypes cultivated in the Arab Center for the Studies of Arid Zones and Dry lands (Sabboura, Damascus countryside, Syria) under the semi arid zones conditions in season 2018. This is to demonstrate the possibility of using them in preeding programs for productivity, as well as finding the relationship between the values of spectral reflectance during the different stages of growth with grain productivity according to mathematical equations based on the values of spectral indices recorded …


Effect Of Some Thermal Treatments In The Growth And Development Of Seedlings Carob (Ceratonia Siliqua L.), تأثير بعض المعاملات الحرارية في نمو وتطور بادرات الخرنوب Ceratonia Siliqua L., Samer Nasser, Talal Ameen, Hafez Mahfoud Mar 2022

Effect Of Some Thermal Treatments In The Growth And Development Of Seedlings Carob (Ceratonia Siliqua L.), تأثير بعض المعاملات الحرارية في نمو وتطور بادرات الخرنوب Ceratonia Siliqua L., Samer Nasser, Talal Ameen, Hafez Mahfoud

Arab Journal of Arid Environments المجلة العربية للبيئات الجافة

This research was conducted at the Scientific Agricultural Research Center (Lattakia/Syria), during the first half of 2015 In order to increase knowledge about the impact of several thermal treatments on some qualitative and quantitative indicators for the growth of shoot and root system of carob (Ceratonia siliqua L.) seedlings. three places were selected with different temperatures degree. The first (Lab, 25±0.5°C ) the second ( Room growth, 20.66±0.5°C ) the third (Open Field, 17.3±0.5°C ). And a private agricultural vessels (Minirhizotron) were used to follow the growth of seedlings roots. The results showed a superiority of Laboratory treatment at …


Estimating Effects Of Radiation Frost On Wheat Using A Field-Based Frost Control Treatment To Stop Freezing Damage, Brenton A. Leske, Ben Biddulph Mar 2022

Estimating Effects Of Radiation Frost On Wheat Using A Field-Based Frost Control Treatment To Stop Freezing Damage, Brenton A. Leske, Ben Biddulph

Grain and Other Field Crops Research Articles

Crop phenotyping experiments have long struggled to have a reliable control treatment that excludes frost and associated freezing damage to plants. Previous attempts used a barrier, such as a removable shelter or cloth to exclude frost. However, these methods were labour intensive and varied in their effectiveness. An automated diesel heater was used to protect field plots of wheat (Triticum aestivum L.) from frost damage. In 2018 and 2019 there were 22 and 33 radiation frost events from July to October at the field site. The heater maintained canopy air temperature above freezing (>0 °C) for the duration of …


Multi-Year Data Analysis And Genomic Selection To Improve The Efficiency Of A Rice Breeding Program, Tommaso Cerioli Mar 2022

Multi-Year Data Analysis And Genomic Selection To Improve The Efficiency Of A Rice Breeding Program, Tommaso Cerioli

LSU Doctoral Dissertations

Plant breeding dramatically improved crops performances during human history and will play a pivotal role in shaping the future of agriculture. However, this activity requires large amount of time and resources. The availability of multi-year datasets and abundant DNA information enables new analyses and breeding approaches that can increase the productivity and efficiency of a breeding program. In recent years, the LSU Rice Breeding Program has implemented marker assisted selection (MAS) and genomic selection (GS) to predict performance of rice genotypes before field testing. The goal of this project was to conduct analyses and test molecular approaches to increase the …


Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang Mar 2022

Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo’s responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and …


Non-Escaping Frost Tolerant Qtl Linked Genetic Loci At Reproductive Stage In Six Wheat Dh Populations, Jingjuan Zhang, Md Shahidul Islam, Yun Zhao, Masood Anwar, Zaid Alhabbar, Maoyun She, Rongchang Yang, Angela Juhasz, Guixiang Tang, Jiansheng Cheng, Darshan L. Sharma, Nathan Height, Ben Biddulph, Hang Liu, Yanjie Jiang, Shengnan Zhai, Xin Hu, Junkang Rong, Yingquan Zhang, Yebo Qin, Qier Liu, Zitong Yu, Yujuan Zhang, Sadegh Balotf, Mirza Dowla, Sonia Afrin, Nandita Roy, Md Resad Mallik, Md Atik Us Saieed, Shanjida Rahman, Nigarin Sultana, Sarah Al-Sheikh Ahmed, Chris Florides, Kefei Chen, Meigin Lu, Jorge E. Mayer, Wujun Ma Feb 2022

Non-Escaping Frost Tolerant Qtl Linked Genetic Loci At Reproductive Stage In Six Wheat Dh Populations, Jingjuan Zhang, Md Shahidul Islam, Yun Zhao, Masood Anwar, Zaid Alhabbar, Maoyun She, Rongchang Yang, Angela Juhasz, Guixiang Tang, Jiansheng Cheng, Darshan L. Sharma, Nathan Height, Ben Biddulph, Hang Liu, Yanjie Jiang, Shengnan Zhai, Xin Hu, Junkang Rong, Yingquan Zhang, Yebo Qin, Qier Liu, Zitong Yu, Yujuan Zhang, Sadegh Balotf, Mirza Dowla, Sonia Afrin, Nandita Roy, Md Resad Mallik, Md Atik Us Saieed, Shanjida Rahman, Nigarin Sultana, Sarah Al-Sheikh Ahmed, Chris Florides, Kefei Chen, Meigin Lu, Jorge E. Mayer, Wujun Ma

Grain and Other Field Crops Research Articles

Reproductive stage frost poses a major constraint for wheat production in countries such as Australia. However, little progress has been made in identifying key genes to overcome the constraint. In the present study, a severe frost event hit two large-scale field trials consisting of six doubled haploid (DH) wheat populations at reproductive stage (young microspore stage) in Western Australia, leading to the identification of 30 robust frost QTL on 17 chromosomes. The major 18 QTL with the phenotype variation over 9.5% were located on 13 chromosomes including 2A, 2B, 2D, 3A, 4A, 4B, 4D, 5A, 5D, 6D, 7A, 7B and …


Arkansas Cotton Variety Test 2021, F. Bourland, A. Beach, B. Milano, C. Kennedy, L. Martin, B. Robertson Feb 2022

Arkansas Cotton Variety Test 2021, F. Bourland, A. Beach, B. Milano, C. Kennedy, L. Martin, B. Robertson

Arkansas Agricultural Experiment Station Research Series

The primary goal of the Arkansas Cotton Variety Test is to provide unbiased data regarding the agronomic performance of cotton varieties and advanced breeding lines in the major cotton-growing areas of Arkansas. This information helps seed companies establish marketing strategies and assists producers in choosing varieties to plant. These annual evaluations will then facilitate the inclusion of new, improved genetic material in Arkansas cotton production. Adaptation of varieties is determined by evaluating the lines at five University of Arkansas System Division of Agriculture research sites (Manila, Keiser, Judd Hill, Marianna, and Rohwer). The 2021 tests at Rohwer were abandoned due …


Berry Anthocyanin, Acid, And Volatile Trait Analyses In A Grapevine-Interspecific F2 Population Using An Integrated Gbs And Rhampseq Genetic Map, Dilmini Alahakoon, Anne Fennell, Zachary Helget, Terry Bates, Avainash Karn, David Manns, Anna Katherine Mansfield, Bruce I. Reisch, Gavin Sacks, Qi Sun, Cheng Zou, Lance Cadle-Dvidson, Jason P. Londo Jan 2022

Berry Anthocyanin, Acid, And Volatile Trait Analyses In A Grapevine-Interspecific F2 Population Using An Integrated Gbs And Rhampseq Genetic Map, Dilmini Alahakoon, Anne Fennell, Zachary Helget, Terry Bates, Avainash Karn, David Manns, Anna Katherine Mansfield, Bruce I. Reisch, Gavin Sacks, Qi Sun, Cheng Zou, Lance Cadle-Dvidson, Jason P. Londo

Agronomy, Horticulture and Plant Science Faculty Publications

Increased map density and transferability of markers are essential for the genetic analysis of fruit quality and stress tolerance in interspecific grapevine populations. We used 1449 GBS and 2000 rhAmpSeq markers to develop a dense map for an interspecific F2 population (VRS-F2) that was derived by selfing a single F1 from a Vitis riparia x ‘Seyval blanc’ cross. The resultant map contained 2519 markers spanning 1131.3 cM and was highly collinear with the Vitis vinifera ‘PN40024’ genome. Quantitative trait loci (QTL) for berry skin color and flower type were used to validate the map. Four rhAmpSeq …


Fddm1 And Fddm2, Two Sgs3-Like Proteins, Function As A Complex To Affect Dna Methylation In Arabidopsis, Shengjun Li, Weilong Yang, Yunfeng Liu, Guangyong Li, Xiang Liu, Yaling Liu, James R. Alfano, Chi Zhang, Bin Yu Jan 2022

Fddm1 And Fddm2, Two Sgs3-Like Proteins, Function As A Complex To Affect Dna Methylation In Arabidopsis, Shengjun Li, Weilong Yang, Yunfeng Liu, Guangyong Li, Xiang Liu, Yaling Liu, James R. Alfano, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

DNA methylation is an important epigenetic modification required for the specific regulation of gene expression and the maintenance of genome stability in plants and animals. However, the mechanism of DNA demethylation remains largely unknown. Here, we show that two SGS3-like proteins, FACTOR OF DNA DEMETHYLATION 1 (FDDM1) and FDDM2, negatively affect the DNA methylation levels at ROS1-dependend DNA loci in Arabidopsis. FDDM1 binds dsRNAs with 50 overhangs through its XS (rice gene X and SGS3) domain and forms a heterodimer with FDDM2 through its XH (rice gene X Homology) domain. A lack of FDDM1 or FDDM2 increased DNA methylation levels …


Qteller: A Tool For Comparative Multi-Genomic Gene Expression Analysis, Margaret R. Woodhouse, Shatabdi Sen, David Schott, John L. Portwood Ii, Michael Freeling, Justin W. Walley, Carson M. Andorf, James Schnable Jan 2022

Qteller: A Tool For Comparative Multi-Genomic Gene Expression Analysis, Margaret R. Woodhouse, Shatabdi Sen, David Schott, John L. Portwood Ii, Michael Freeling, Justin W. Walley, Carson M. Andorf, James Schnable

Center for Plant Science Innovation: Faculty Publications

Motivation: Over the last decade, RNA-Seq whole-genome sequencing has become a widely used method for measuring and understanding transcriptome-level changes in gene expression. Since RNA-Seq is relatively inexpensive, it can be used on multiple genomes to evaluate gene expression across many different conditions, tissues and cell types. Although many tools exist to map and compare RNA-Seq at the genomics level, few web-based tools are dedicated to making data generated for individual genomic analysis accessible and reusable at a gene-level scale for comparative analysis between genes, across different genomes and meta-analyses. Results: To address this challenge, we revamped the comparative gene …


Proceedings Of The 2022 Passion Fruit Conference: Growing The U.S. Passion Fruit Industry A Strategic Conference For: Growers, Marketers, Researchers, And Stakeholders, Eric T. Stafne Jan 2022

Proceedings Of The 2022 Passion Fruit Conference: Growing The U.S. Passion Fruit Industry A Strategic Conference For: Growers, Marketers, Researchers, And Stakeholders, Eric T. Stafne

CREC Publications

We are pleased to deliver these proceedings of the passion fruit conference that was held in Homestead, Florida on June 14 and 15, 2022. It was a culmination of a lot of hard work by many people, most of them listed in the previous page. However, it would not have been a success without the attendees who came from various parts of the country to share their experiences growing passion fruit. What follows in these proceedings are the slides of the presentations with by notes taken by attendees along with notes taken of discussion regarding the five priority topic areas: …


Determinants Of Population Genetic Structure In Chamaecrista Fasciculata (Michx.) Greene (Fabaceae) In The Southeastern United States, Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace Jan 2022

Determinants Of Population Genetic Structure In Chamaecrista Fasciculata (Michx.) Greene (Fabaceae) In The Southeastern United States, Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace

Biological Sciences Faculty Publications

Chamaecrista fasciculata is a widely distributed, phenotypically variable species in the eastern U.S. Whereas studies have demonstrated genetic structure and local adaptation in northern areas of its distribution, there has been no comparison of genetic variability among populations at the southern extent where phenotypic variation is more complex. We characterized genetic variation at 14 microsatellite loci for populations in Mississippi and Alabama and compared this to variation in a phenotypic trait, leaf pubescence. Geographic distance, climatic variables, and elevation were evaluated as factors to explain the observed patterns of genetic diversity. A significant amount of variation (19%) resided among populations, …


The Challenges Of Growing Orchids From Seeds For Conservation: An Assessment Of Asymbiotic Techniques, Devani Jolman, Martín I. Batalla, Alexis Hungerford, Pryce Norwood, Noah Tait, Lisa E. Wallace Jan 2022

The Challenges Of Growing Orchids From Seeds For Conservation: An Assessment Of Asymbiotic Techniques, Devani Jolman, Martín I. Batalla, Alexis Hungerford, Pryce Norwood, Noah Tait, Lisa E. Wallace

Biological Sciences Faculty Publications

Lewis Knudson first successfully germinated orchid seeds asymbiotically on artificial medium in 1922. While many orchid species have since been grown asymbiotically, the tremendous variation in how species respond to artificial medium and growth conditions ex situ has also become apparent in the past century. In this study, we reviewed published journal articles on asymbiotic orchid seed germination to provide a summary of techniques used and to evaluate if these differ between terrestrial and epiphytic species, to identify areas where additional research is needed, and to evaluate whether asymbiotic germination could be used more often in ex situ conservation. We …


Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben Jan 2022

Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben

Electronic Theses and Dissertations

Grapevine is one of the most important fruit crops in the world, responsible for billions in global sales annually. The largest threat to grapevine and other crop production is global climate change resulting human activities. This brings unpredictable and drastic changes in ambient air temperatures to many climates in which grapes are grown. Lower temperatures and inclement weather are already responsible for millions in lost revenue due to tissue damage of established plants. Thus, protecting grapevine crops from weather-related damage is the biggest concern to growers aside from pathogen- and diseaserelated crop damage. The primary mechanism for winter survival in …


Characterizing Drought Adaptations, Phenotypic Plasticity, And Fixed Gene Expression Patterns Within Quercus, James K. Rauschendorfer Jan 2022

Characterizing Drought Adaptations, Phenotypic Plasticity, And Fixed Gene Expression Patterns Within Quercus, James K. Rauschendorfer

Dissertations, Master's Theses and Master's Reports

This dissertation was written on topics related to the genus Quercus with a primary focus on Quercus ellipsoidalis (northern pin oak) and Quercus rubra (northern red oak). Within this dissertation are chapters related to the setup of experimental common gardens within the Ford and Kellogg experimental forest, a literature review describing drought adaptations of Quercus sect. Lobatae (red oak group), identification of transcription factors within the Q. robur (English oak) and Q. rubra genomes, a study comparing leaf trait phenotypic plasticity of Q. ellipsoidalis and Q. rubra, and an RNA-seq experiment studying ecological speciation between Q. ellipsoidalis and Q. …